长期有机与无机肥配施的黄壤稻田土壤细菌群落结构特征

  【目的】  比较长期施用不同肥料黄壤稻田土壤细菌群落结构的差异,剖析不同肥料维持土壤细菌群落多样性的作用及其机理,为农田施肥管理提供理论依据。  【方法】  以农业农村部贵州耕地保育与农业环境科学观测试验站为依托,采用高通量测序技术,分析连续24年不施肥(CK)、全量化肥(NPK)、1/4牛厩肥+3/4化肥(1/4M+3/4NP)、1/2牛厩肥+1/2化肥(1/2M+1/2NP)和全量牛厩肥(M)处理黄壤稻田土壤细菌群落组成及多样性,并揭示其主要环境影响因子。  【结果】  不同施肥处理土壤细菌α多样性指数分析结果显示,长期施用有机肥提高了土壤细菌多样性指数(Shannon)、优势度指数(Simpson)和均匀度指数(Pielou),对丰富度指数(Chao1)影响较小。与CK相比,长期施肥不同程度地提高了变形菌门(Proteobacteria)的相对丰度,化肥的作用更明显。与CK和NPK相比,施用有机肥处理降低了棒状杆菌门(Rokubacteria)和亚硝酸盐氧化菌门(Nitrospinae)相对丰度,提高了拟杆菌门(Bacteroidetes)相对丰度,其他菌门变化不明显(P<0.05)。土壤细菌群落结构主成分分析结果表明,施用有机肥1/4M+3/4NP和1/2M+1/2NP处理土壤环境较为相似,细菌群落组成相似度较高,而CK和NPK处理土壤环境相似,细菌群落组成相似度高。土壤细菌群落结构组成与土壤环境因子冗余分析显示,土壤理化性质对细菌群落结构的影响重要性由大到小依次为全氮、碱解氮、速效钾、pH、有效磷、全磷,其中全氮、碱解氮和速效钾是关键因素。  【结论】  长期施用有机肥能够提高黄壤稻田土壤肥力,改变细菌生长环境,进而改变细菌群落结构组成,提高细菌群落多样性,促进土壤生态系统稳定和健康。     

长期施肥红壤稻田有机碳演变规律及影响因素

利用1981年起设置的水稻土长期施肥定位试验,分析了CK(不施肥)、N(单施氮肥)、NPK(氮磷钾配施)、NPK2(2倍氮磷钾配施)和NPKM(有机无机肥配施)等施肥措施下土壤有机碳的演变规律及其与作物产量和土壤养分(全氮、碱解氮、全磷、速效磷、全钾和速效钾)的相关性.结果表明:试验30年后,各个处理的土壤有机碳含量均有上升,其中CK、N、NPK、NPK2和NPKM的土壤有机碳在试验30年分别比试验前增加18.95％、17.72％、23.36％、16.92％和32.68％.与CK处理相比,NPK、NPK2和NPKM处理的土壤有机碳平均提高了4.09％、4.03％和25.68％.土壤有机碳含量与水稻产量呈显著相关(P＜0.001),相关系数r为0.410,这说明土壤有机碳含量的增加可以促进水稻增产.土壤有机碳与土壤养分中的碱解氮、速效磷和全磷含量均表现出极显著相关(P＜0.001),相关系数r分别为0.452、0.559和0.487,但是与钾含量相关不显著.这表明:有机无机肥配施可以持续快速提高红壤性水稻土的有机碳含量,同时在有机无机肥配施过程中应适当增施钾肥,从而促进土壤肥力平衡和维持作物高产稳产,实现农业可持续性.     

化肥配施有机肥对花生根际细菌群落结构及共存网络的影响

为评估化肥配施有机肥对花生根际细菌群落的影响，田间试验条件下研究了不施肥(CK)、施化学氮磷钾肥(NPK)和化学氮磷钾肥+有机肥配施(NPKM)对花生根际细菌群落多样性、结构和共存网络的影响。结果表明：NPKM处理下花生根际土养分状况明显改善，特别是有效磷的含量，较CK和NPK处理分别提高了5.31倍～12.16倍和3.24倍～6.50倍；而NPK处理下花生根际土养分状况并没有明显提升；Source Tracker分析显示，NPKM处理下根际细菌群落中只有2.1%～5.5%的物种来源于有机肥自身，但其花生根际细菌群落多样性在生育期前期要明显高于CK和NPK处理，而CK和NPK处理下根际细菌群落多样性差异不大；限制性主成分分析(CAP)显示，施肥措施和生育期均对根际细菌群落结构产生了显著影响，NPKM处理下花生根际土中富集了属于根瘤菌目(Rhizobiales)、梭菌目(Clostridiales)和芽孢杆菌目(Bacillales)这一类根际促生菌，根际细菌共存网络结构更加复杂，且网络中重要连接点的比例要也明显高于CK和NPK处理网络。可见，化肥配施有机肥有助于构建一个健康稳定的根际细...     

氮磷钾对春茶光合生理及氨基酸组分的影响

【目的】当前为追求茶园高产盲目施肥、滥用化肥造成生态环境破坏的现象较为严重,因此如何合理施肥显得尤为重要,本文通过研究氮、磷、钾不同水平及其配比对春茶净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间CO2浓度(Ci)和水分利用效率(WUE)等光合生理指标和春茶新梢氮、磷、钾含量,氨基酸总量及其组分的影响,为茶园平衡施肥和精准施肥提供理论指导。【方法】采取单因素试验设计,设氮、磷、钾各3个水平及其配比和对照,共14个处理(N1、N2、N3、P1、P2、P3、K1、K2、K3、NP、NK、PK、NPK、CK),在茶树新梢长至1芽3叶时采样,用Li-6400便携式光合测定仪测定茶树的光合生理指标,用氨基酸自动分析仪测定春茶氨基酸总量及组分含量,分别采用凯氏定氮法、钒钼黄比色法和原子吸收分光光度法测定茶树新梢的全氮、全磷和全钾含量。分析不同施肥处理对茶树光合生理指标和春茶氨基酸总量及其组分的影响,并通过逐步回归分析建立Pn与茶树新梢氮、磷、钾含量,Tr、Gs和Ci的回归方程。【结果】NPK处理茶树Pn比CK提高9.70%;P2、P3和NPK处理的Tr显著增加,比CK分别提高10.60%、14.92%、14.25%;NPK处理的Gs显著增加,比CK高23.15%,同时Gs随不同施肥水平表现出与蒸腾速率较一致的趋势,二者的相关性极显著;Ci随氮、磷、钾施用量的增加而上升;WUE随氮、磷、钾施用量的增加而下降。NPK处理的茶树新梢的氮、磷含量较高,相同施肥处理的茶树新梢的磷含量低于氮、钾。逐步回归分析显示,茶树新梢磷含量,Ci和Gs对Pn的直接作用较大,直接通径系数分别为0.3688、-0.8139和0.4677,Pn=12.955+26.624P-0.087Ci+38.233Gs;NPK处理下春茶氨基酸总量及茶氨酸、天冬氨酸、苏氨酸、丝氨酸和胱氨酸等主要氨基酸组分的含量分别比对照提高了27.88%、26.60%、35.78%、35.06%、24.41%和24.41%,与对照差异显著。【结论】新梢磷含量和气孔导度对春茶净光合速率的提高有直接的促进作用,而胞间CO2浓度对净光合速率有一定的消减作用。氮磷钾配施可显著提升茶树新梢叶片的气孔导度并保证茶树新梢中较高的磷含量,从而提高了茶树的净光合速率。氮磷钾肥配施能显著提高春茶氨基酸总量及其组分含量,提高了春茶的品质,表明茶叶中氨基酸总量和茶氨酸等重要组分含量的提高是氮、磷、钾营养共同作用的结果。因此,氮磷钾配合施用可以提高春茶的产量和品质。     

Long-term fertilization regimes affect bacterial community structure and diversity of an agricultural soil in northern China

Background, Aims, and Scope  Knowledge about shifts of microbial community structure and diversity following different agricultural management practices could improve our understanding of soil processes and thus help us to develop sound management strategies. A long-term fertilization experiment was established in 1989 at Fengqiu (35°00′N, 114°24′E) in northern China. The soil (sandy loam) is classified as aquic inceptisols and has received continuous fertilization treatments since then. The fertilization treatments included control (CK, no fertilizer), chemical fertilizers nitrogen (N) and potassium (K) (NK), phosphorous (P) and K (PK), NP, NPK, organic manure (OM), and half chemical fertilizers NPK plus half organic manure (1/2NPKOM). The objective of this study was to examine if the microbial community structure and diversity were affected by the long-term fertilization regimes. Materials and Methods  Soil samples were collected from the long-term experimental plots with seven treatments and four replications in April 2006. Microbial DNAs were extracted from the soil samples and the 16S rRNA genes were PCR amplified. The PCR products were analyzed by DGGE, cloning and sequencing. The bacterial community structures and diversity were assessed using the DGGE profiles and the clone libraries constructed from the excised DGGE bands. Results  The bacterial community structure of the OM and PK treatments were significantly different from those of all other treatments. The bacterial community structures of the four Ncontaining treatments (NK, NP, NPK and 1/2NPKOM), as well as CK, were more similar to each other. The changes in bacterial community structures of the OM and PK treatments showed higher richness and diversity. Phylogenetic analyses indicated that Proteobacteria (30.5%) was the dominant taxonomic group of the soil, followed by Acidobacteria (15.3%), Gemmatimonadetes (12.7%), etc. Discussion  Irrespective of the two fertilization treatments of OM and PK, the cluster analysis showed that bacterial communities of the remaining five treatments of CK, NK, NP, NPK and 1/2NPKOM seemed to be more similar to each other, which indicated the relatively weak effects of the four N-containing treatments on soil bacterial communities. N fertilizer may be considered as a key factor to counteract the effects of other fertilizers on microbial communities. Conclusions  Our results show that long-term fertilization regimes can affect bacterial community structure and diversity of the agricultural soil. The OM and PK treatments showed a trend towards distinct community structures, higher richness and diversity when compared to the other treatments. Contrasting to the positive effects of OM and PK treatments on the bacterial communities, N fertilizer could be considered as a key factor in the soil to counteract the effects of other fertilizers on soil microbial communities. Recommendations and Perspectives  Because of the extremely high abundance and diversity of microorganisms in soil and the high heterogeneity of the soil, it is necessary to further examine the effects of fertilization regimes on microbial community and diversity in different type soils for comprehensively understanding their effects through the appropriate combination of molecular approaches. ESS-Submission Editor: Chengrong Chen, PhD (c.chen@griffith.edu.au)     

东北黑土nirS型反硝化细菌群落和网络结构对长期施用化肥的响应

【目的】探明长期施用氮磷钾化肥对东北黑土农田土壤nirS型反硝化细菌群落和网络结构的影响,为更加合理的肥料配施提供理论依据。【方法】基于农业农村部黑龙江耕地保育与农业环境科学观测实验站平台,选取不施肥(NoF)、氮肥(N)、磷肥(P)、钾肥(K)、氮钾肥(NK)、氮磷肥(NP)、磷钾肥(PK)、氮磷钾肥(NPK)8个施肥处理,借助荧光定量PCR、Illumina MiSeq高通量测序和分子生态网络技术,分析东北黑土nirS型反硝化细菌丰度、群落及网络结构,及影响反硝化细菌群落变异的主要环境因子。【结果】1)长期施用氮肥均在显著增加nirS基因拷贝数的同时,降低了nirS型反硝化细菌的群落多样性,而磷、钾肥对其影响并不显著。2)Proteobacteria是所有处理中的优势反硝化细菌门,相对丰度为16.96%~27.34%,且氮肥的施用促进了隶属于该菌门中Bradyrhizobium的生长。3)PCoA分析结果显示,8个施肥处理nirS型反硝化细菌群落主要分成施氮肥和不施氮肥两组,说明长期氮肥的施用显著改变了东北黑土反硝化细菌的群落结构。结合Mantel test的结果可知,土壤pH是影响nirS型反硝化细菌群落改变的主要因素。4)分别构建施氮肥和不施氮肥的反硝化细菌分子生态网络,发现施氮肥和不施氮肥网络结构存在很大差异,长期施用氮肥明显简化了nirS型反硝化细菌的网络结构,同时使其网络结构稳定性降低,易受外界环境扰动。【结论】尽管施用化学氮肥有利于土壤养分的增加,但其土壤nirS型反硝化细菌群落及网络结构发生了较大改变,而磷、钾肥的施用对反硝化细菌群落无显著影响。本试验结果为进一步研究东北黑土区农田土壤反硝化微生物对不同施肥管理的响应机制提供了重要科学依据。     

长期施用化肥对塿土微生物多样性的影响

【目的】土壤微生物在土壤有机质分解、营养循环、植物生长等方面都发挥着重要作用,研究长期施用化肥对其产生的影响可为农田合理施用化肥、培肥土壤和高产高效可持续性农业生产提供理论依据。【方法】以陕西杨凌"国家黄土肥力与肥料效益监测基地"的长期定位试验为基础,利用BIOLOG分析并结合常规分析研究了6种长期不同化肥施用方式不施肥(CK)、单施氮肥(N)、氮钾配施(NK)、磷钾配施(PK)、氮磷配施(NP)和氮磷钾配施(NPK)对土土壤微生物量和微生物功能多样性的影响。【结果】与不施肥CK相比,长期单施氮肥(N)的SMBC、SMBN没有显著变化,但明显降低了土壤微生物商和土壤微生物对氮素的利用;NP和NPK配施能够显著增加土SMBC和SMBN含量,明显提高了土壤微生物商,使土壤微生物种群结构发生了明显变化但土壤微生物对氮素的利用没有显著提高;长期偏施肥处理(NK、PK)的SMBC、SMBN和微生物商虽轻微增加,但土壤微生物种群结构没有显著改变。BIOLOG分析结果显示施磷处理(PK、NP和NPK)对微生物代谢活性的促进作用较大且在培养初期代谢活性较不施磷处理(CK、N和NK)增加显著。长期单施氮肥(N)对于提高土壤微生物多样性没有显著作用而其他化肥施用处理可以提高土土壤微生物群落的碳源利用能力、物种的丰富度和优势度,其中NP和NPK处理配施效果最好。化肥施用对土土壤微生物群落的均匀度没有显著影响。主成分分析的结果表明不同处理的土壤微生物对碳源利用表现出显著差异,氮磷养分的差异是产生分异的主要原因。【结论】土区小麦玉米轮作下,平衡施肥(NP或NPK配施)对于改善农田土壤微生物特性具有良好作用。     

设施栽培番茄的氮磷钾肥料效应研究

采用"3414"肥料试验设计开展了设施栽培番茄的氮、磷、钾肥料效应研究。结果表明,在高肥力土壤上适量施用氮、磷、钾肥均可增加番茄产量,但过量施用会降低其产量;钾肥的增产作用大于磷肥,氮肥的作用较小;氮、钾肥和磷、钾肥配施可增加番茄产量,而氮、磷肥配施降低产量。氮、磷(P_2O_5)和钾(K_2O)最佳经济施肥量分别为119.0、50.4和375.6 kg/hm~2,施肥比例为1∶0.42∶3.16。不同施氮量对番茄硝酸盐和亚硝酸盐含量的影响不规律,增施磷、钾肥番茄硝酸盐含量呈先增加后减少趋势;氮、磷肥和磷、钾肥配施可降低番茄硝酸盐和亚硝酸盐含量。氮、钾肥和磷、钾肥配施提高了番茄可溶性糖含量,氮、磷肥和磷、钾肥配施降低了番茄总酸含量,氮、钾肥配施则有增加番茄总酸含量的趋势,氮、钾肥和磷、钾肥配施均可提高番茄Vc含量。氮、磷、钾肥料合理配施对番茄产量和品质的提高具有重要作用。     

Animal-based organic nutrition can substitute inorganic fertigation in soilless-grown grape tomato

Purpose: In recent years, interest in plant nutrition research has arisen with a strong focus on organic forms. The aim of this study was to determine the effect of different organic fertilizers on growth, yield, fruit quality and polyphenol content in soilless grown grape tomatoes under greenhouse conditions.

Materials and methods: Tomato plants were subjected to three organic nutrient solutions, which consisted of different mixtures of several OMRI (Organic Materials Review Institute) certified nitrogen fertilizers of industrially processed residues: Treatment I: solid and soluble liquid fertilizers of animal raw materials, natural potassium sulphate-non-synthetic, and calcium chloride; Treatment II: solid and soluble liquid fertilizers of animal raw materials, by-product of marine raw material (soluble liquid), natural potassium sulphate-non-synthetic, and calcium chloride; and Treatment III: solid fertilizers of animal raw materials, natural potassium sulphate-non-synthetic, and calcium chloride to 100% [0-30 days after transplanting (DAT)], 125% [31-80 DAT], and 150% [>81 DAT]. The Steiner solution (SS) was used as a control (Treatment IV).

Results: Yield did not differ between organic and conventional treatments, ranging from 3.04 to 3.35 kg m^-2 while fresh weight in organic treatments was 3.14 compared to 3.2 kg m^-2 in plants fed with the SS. No significant differences in plant height or fruit quality were found. The application of organic fertilizers positively affected the total hydrolysable and condensed polyphenols of tomato fruits compared to the control. Twelve phenolic compounds were identified, highlighting 3-Caffeoylquinic acid, salvianolic acid and 5,6-Dihydroxy-7,8,3’,4’-tetramethoxyflavone (Treatment I) and Medioresinol (Lignan) (Treatment II).

Conclusions: The results indicated that organic fertilization through animal-based fertilizer application is a feasible alternative for grape tomato production under greenhouse conditions.     

长期施氮、磷、钾化肥对玉米产量及土壤肥力的影响

以1990年建立的国家褐潮土土壤肥力与肥料效益长期监测基地(北京昌平站)的长期肥料定位试验为研究平台,研究了不同施肥制度对玉米产量和土壤肥力的影响。结果表明,长期均衡地施NPK肥或NPK与有机肥配施,可以显著提高玉米产量和土壤有机质、全氮、全磷、速效氮、速效磷、速效钾等肥力指标,并能提高土壤微量元素的含量;而不均衡施肥(N、NK、NP、PK)导致相应的营养元素的耗竭。相关分析表明,在褐潮土上增施磷肥和有机肥对提高玉米产量具有重要的作用。     

土区长期施肥对小麦-玉米轮作体系钾素平衡与钾库容量的影响

本文研究了土区小麦-玉米轮作体系长期氮磷钾化肥不同配合施用方式及氮磷钾化肥与秸秆或有机肥配合施用对钾素平衡以及土壤钾库的影响。试验包括9个处理,分别为不施肥（CK）、单施氮（N）、氮钾（NK）、磷钾（PK）、氮磷（NP）、氮磷钾（NPK）、氮磷钾配合一季秸秆还田（SNPK）、氮磷钾配合低量有机肥（M1NPK）和高量有机肥（M2NPK）。结果表明,除NK、PK和M2NPK处理外,其它处理小麦和玉米钾的携出量均大于钾的投入量,导致土壤钾素处于亏缺状态,20年累计亏缺量为6174333 kg/hm2。与试验前相比,长期施肥种植没有显著影响土壤全钾含量; 长期施用钾肥显著提高土壤速效钾含量,但长期不施钾肥处理的土壤速效钾含量也未显著降低; 无论施钾与否土壤非交换性钾（Mactotal K）以及非交换性钾中更容易被HNO3溶解提取的钾（Step K）均明显低于试验前水平。表明土壤非交换性钾可以作为该土壤钾素消耗的指标。考虑到施钾肥的经济投入和现有资源高效利用（如秸秆、有机肥）,从长远的角度出发,维持土壤钾素肥力以及土地可持续生产力,土区小麦-玉米轮作体系采用秸秆全部还田或施有机肥是必要的。     

Effect of long‐term fertilization and manuring on potassium release properties in a Typic Ustochrept

A long‐term fertilizer experiment, over 27 years, studied the effect of mineral fertilizers and organic manures on potassium (K) balances and K release properties in maize‐wheat‐cowpea (fodder) cropping system on a Typic Ustochrept. The treatments consisted of control, 100% nitrogen (100% N), 100% nitrogen and phosphorus (100% NP), 50% nitrogen, phosphorus, and potassium (50% NPK), 100% nitrogen, phosphorus, and potassium (100% NPK), 150% nitrogen, phosphorus, and potassium (150% NPK), and 100% NPK+farmyard manure (100% NPK+FYM). Nutrients N, P, and K in 100% NPK treatment were applied at N: 120 kg ha^—1, P: 26 kg ha^—1, and K: 33 kg ha^—1 each to maize and wheat crops and N: 20 kg ha^—1, P: 17 kg ha^—1, and K: 17 kg ha^—1 to cowpea (fodder). In all the fertilizer and manure treatments removal of K in the crop exceeded K additions and the total soil K balance was negative. The neutral 1 N ammonium acetate‐extractable K in the surface soil (0—15 cm) ranged from 0.19 to 0.39 cmol kg^—1 in various treatments after 27 crop cycles. The highest and lowest values were obtained in 100% NPK+FYM and 100% NP treatments, respectively. Non‐exchangeable K was also depleted more in the treatments without K fertilization (control, 100% N, and 100% NP). Parabolic diffusion equation could describe the reaction rates in CaCl₂ solutions. Release rate constants (b) of non‐exchangeable K for different depth of soil profile showed the variations among the treatments indicating that long‐term cropping with different rates of fertilizers and manures influenced the rate of K release from non‐exchangeable fraction of soil. The b values were lowest in 100% NP and highest in 100% NPK+FYM treatment in the surface soil. In the sub‐surface soil layers (15—30 and 30—45 cm) also the higher release rates were obtained in the treatments supplied with K than without K fertilization indicating that the sub‐soils were also stressed for K in these treatments.     

Soil Total Nitrogen and Natural 15Nitrogen in Response to Long-Term Fertilizer Management of a Maize–Wheat Cropping System in Northern China

The Fengqiu long-term field experiment was established to examine effects of organic manure and mineral fertilizers on soil total nitrogen (N) and natural ¹⁵N abundance. Fertilizer regimes include organic manure (OM), one-half N from organic manure plus one-half N from mineral N fertilizer (1/2OMN), mineral fertilizers [N–phosphorus (P)–potassium (K), NP, NK, PK], and a control. Organic manure (OM and 1/2OMN) significantly increased soil total N and δ¹⁵N, which was expected as a great amount of the N applied remained in soils. Mineral NPK fertilizer and mineral NP fertilizer significantly increased total N and slightly increaed δ¹⁵N. Phosphorus-deficient fertilization (NK) and N-deficient fertilization (PK) had no effect on soil total N. Significantly greater δ¹⁵N was observed in the NK treatment as compared to the control, suggesting that considerable N was lost by ammonia (NH₃) voltalization and denitrification in this P-deficiency fertilization regime.     

不同肥料/改良剂对冷泥田水稻生长、养分吸收及土壤性质的影响

【目的】西南地区冷泥田的水稻生长不良可归因于多种障碍因子,包括土壤物理、化学和生物因子。本文针对冷泥田影响水稻生长的土壤化学障碍因子,研究了不同肥料或改良剂对冷泥田水稻生长、养分吸收和土壤性质的影响,旨在为这类土壤的培肥、改良利用和水稻高产提供科学依据和实用技术。【方法】试验地点选在四川省东南地区冷泥田集中的泸县,试验历时2年。试验设无肥对照(CK)、全量化肥(NPK)、NPK+泥炭(NPK+Pe)、NPK+商品有机肥(NPK+COF)、NPK+鸡粪(NPK+CM)、NPK+硅肥(NPK+Si)、NPK+石灰(NPK+L)、NPK+硅钙肥(NPK+Si Ca),NPK+锌肥(NPK+Zn)9个处理。在水稻收获前采集代表性植株样,调查水稻农艺性状,分析水稻对氮、磷和钾的吸收量;水稻收获后采集土壤样品,测定不同处理的土壤p H、全氮、有效磷、速效钾、有机质、还原性物质总量、活性还原物质以及亚铁和亚锰含量。【结果】结果表明,在所有处理中,NPK+L和NPK+Si Ca处理可改善水稻的产量构成因子,稻谷产量比NPK处理显著提高15.40%和10.64%;而其他处理的增产效果则均不显著。NPK+L和NPK+Si Ca处理的土壤p H明显高于其他处理,土壤还原性物质总量和活性还原物质(含Fe2+和Mn2+)含量显著降低;NPK+L和NPK+Si Ca处理可增加土壤养分的有效性,促进水稻对氮、磷、钾养分的吸收,改善土壤的化学性质。同时,土壤分析结果表明,该试验土壤的有效钙和有效硅均缺乏,而NPK+L和NPK+Si Ca处理增加了土壤的有效钙和有效硅,对提高水稻产量有重要贡献。有机肥对土壤相关性质的影响与石灰和硅钙肥相反,特别是鸡粪。施用锌肥对水稻有一定的增产效果,但差异不显著。水稻收获后土壤中氮、磷、钾养分残留量与施肥量和水稻吸收携出量密切相关。【结论】对长期淹水地势低洼的酸性冷泥田来说,石灰和硅钙肥是比较理想的土壤改良剂,能同时起到改善土壤性质和提高作物产量的效果;而有机肥,特别是未腐熟的有机肥或有机物料,则不宜在冷泥田中施用或大量施用。     

西湖平原区连续13年定位施肥对麦、稻产量及土壤肥力的影响

以浙江省水网平原水稻主产区土壤为对象,通过定位试验,研究了连续13年的不同施肥处理对麦稻产量、土壤养分状况和物理性状的影响。结果表明,化肥配施有机肥可显著提高麦、稻产量; 不同施肥处理的长期定位试验土壤有机质含量和全氮均呈上升趋势,增幅依次为: 栏肥+NPK秸秆+NPKNPK秸秆栏肥CK处理; 土壤碱解氮和速效磷也呈增加趋势,以栏肥+NPK处理的增幅最为明显。土壤物理性状的分析表明,长期施肥均能明显增加土壤水稳性团粒含量和土壤孔隙度。经土壤养分平衡分析,栏肥+NPK、秸秆+NPK和NPK处理的氮和磷呈现盈余,秸秆和CK处理氮和磷亏缺; 栏肥+NPK和秸秆+NPK处理钾基本平衡,NPK、秸秆、栏肥和CK处理钾严重亏缺。长期定位试验进一步证明有机肥与氮、磷、钾化肥长期配合施用可实现当地农作物持续稳产,农田施肥管理要注意适当减少氮、磷投入,增加钾肥施用量,保持农田土壤养分平衡。     

长期施肥对棕壤铁形态及其有效性的影响

【目的】利用沈阳农业大学棕壤肥料长期定位试验,研究不同施肥处理对耕层土壤酸碱度（pH）、氧化还原电位（Eh）和有机质的影响,探讨不同施肥条件下土壤游离态氧化铁、无定形氧化铁、亚铁总量和有效铁含量的变化以及与pH、Eh和有机质的关系。 【方法】本文选取试验处理为CK（不施肥）、N（氮肥）、NP（氮磷肥配施）、NPK（氮磷钾肥配施）、M（有机肥）、MN（有机肥与氮肥配施）、MNP（有机肥与氮磷肥配施）、MNPK（有机肥与氮磷钾肥配施）。在2014年大豆收获期,采集了不同施肥处理0-20 cm耕层土壤样品,分析了土壤pH、Eh、有机质含量以及游离态氧化铁、无定形氧化铁、亚铁和有效铁含量。 【结果】与1979年原始土壤相比,所有处理土壤pH显著降低了0.6~1.4个单位。不施肥处理土壤有机质下降了11.5%,化肥处理有机质略有下降,而有机肥处理有机质含量显著增加。所有处理有效铁含量显著增加,化肥有机肥配施增加幅度更大;与2014年不施肥处理相比,施用氮肥处理土壤pH最低,而氮肥配施磷、钾和有机肥中的一种或几种pH又有所上升,其中配施有机肥效果最明显;施用化肥处理土壤Eh增加,而施用有机肥处理Eh则下降。施用化肥土壤游离态氧化铁和有效铁含量增加,亚铁含量下降,而施用有机肥土壤游离态氧化铁降低,亚铁总量及有效铁含量增加。 【结论】经过长期耕作和施肥,土壤pH显著下降,有效铁含量显著增加。长期不施肥土壤有机质显著下降。施用氮肥土壤酸化趋势明显,磷、钾和有机肥配施能够缓解氮肥引起的酸化现象。施用有机肥显著降低土壤氧化还原电位,有利于氧化态铁向还原态铁转化,更有利于增加土壤有效性铁的含量。     

Long-term fertilization and residue return affect soil stoichiometry characteristics and labile soil organic matter fractions

Ecological stoichiometry provides the possibility for linking microbial dynamics with soil carbon (C), nitrogen (N), and phosphorus (P) metabolisms in response to agricultural nutrient management. To determine the roles of fertilization and residue return with respect to ecological stoichiometry, we collected soil samples from a 30-year field experiment on residue return (maize straw) at rates of 0, 2.5, and 5.0 Mg ha^-1 in combination with 8 fertilization treatments:no fertilizer (F0), N fertilizer, P fertilizer, potassium (K) fertilizer, N and P (NP) fertilizers, N and K (NK) fertilizers, P and K (PK) fertilizers, and N, P, and K (NPK) fertilizers. We measured soil organic C (SOC), total N and P, microbial biomass C, N, and P, water-soluble organic C and N, KMnO₄-oxidizable C (KMnO₄-C), and carbon management index (CMI). Compared with the control (F0 treatment without residue return), fertilization and residue return significantly increased the KMnO₄-C content and CMI. Furthermore, compared with the control, residue return significantly increased the SOC content. Moreover, the NPK treatment with residue return at 5.0 Mg ha^-1 significantly enhanced the C:N, C:P, and N:P ratios in the soil, whereas it significantly decreased the C:N and C:P ratios in soil microbial biomass. Therefore, NPK fertilizer application combined with residue return at 5.0 Mg ha^-1 could enhance the SOC content through the stoichiometric plasticity of microorganisms. Residue return and fertilization increased the soil C pools by directly modifying the microbial stoichiometry of the biomass that was C limited.     

Abundance and community structure of ammonia-oxidizing archaea and bacteria in an acid paddy soil

Nitrification is essential to the nitrogen cycle in paddy soils. However, it is still not clear which group of ammonia-oxidizing microorganisms plays more important roles in nitrification in the paddy soils. The changes in the abundance and composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were investigated by real-time PCR, terminal restriction fragment length polymorphism, and clone library approaches in an acid red paddy soil subjected to long-term fertilization treatments, including treatment without fertilizers (CT); chemical fertilizer nitrogen (N); N and potassium (NK); N and phosphorus (NP); N, P, and K (NPK); and NPK plus recycled crop residues (NPK+C). The AOA population size in NPK+C was higher than those in CT, while minor changes in AOB population sizes were detected among the treatments. There were also some changes in AOA community composition responding to different fertilization treatments. Still few differences were detected in AOB community composition among the treatments. Phylogenetic analysis showed that the AOA sequences fell into two main clusters: cluster A and cluster soil/sediment. The AOB composition in this paddy soil was dominated by Nitrosospira cluster 12. These results suggested that the AOA were more sensitive than AOB to different fertilization treatments in the acid red paddy soil.     

Volatile losses of sulphur from oven drying lucerne (Medicago sativa L.): Effects of sulphur,nitrogen, phosphorus and potassium supply

Lucerne was grown in nutrient solutions at adequate and “deficient”; levels of sulphur, nitrogen, phosphorus or potassium. Volatile losses of sulphur were then measured when shoots and roots were oven dried at 80°C for 48 hours.

Small but significant losses of sulphur occurred during oven drying. Losses from shoots ranged from 4.2 to 13.9 μgS/g dry weight, which represented from 0.38 to 0.66% of the total sulphur content of the shoot. Losses from roots ranged from 12.0 to 47.8 μgS/g dry weight, representing 0.82 to 1.77% of the total sulphur content of the root. Decreases in supply of nitrogen, phosphorus, potassium or sulphur generally decreased the amount of volatile sulphur lost by oven drying shoots. Losses from roots generally decreased when supply of sulphur decreased, but increased when nitrogen and potassium supply decreased, and were generally unaffected by phosphorus supply.

The organic sulphur concentration in the tissue was linearly correlated with absolute losses of sulphur (r = 0.799** for shoots; r = 0.822** for roots), the amount of sulphur lost per unit dry weight (r = 0.469* for shoots; r = 0.381* for roots) and the percentage of the total sulphur released as volatile sulphur (r = 0.937** for shoots; r = 0.970** for roots). By contrast, the total sulphur concentration in the tissue was linearly correlated only with the amount of sulphur lost per unit dry weight ( r = 0.704** for shoots; r = 0.723** for roots).     

长期定位施肥对潮土磷素形态和有效性的影响

研究长期定位施肥对潮土各形态磷含量变化及磷有效性的影响,为潮土合理施用磷肥提供理论依据.长期定位施肥试验开始于1990年,设CK(不施肥)、N2(单施尿素)、N2P(不施钾肥)、N2K(不施磷肥)、N1PK(低量氮肥和磷钾肥)、N2PK(平衡施肥)、N3PK(中高量施肥)、N4PK(高量氮肥和磷钾肥)、N2PKM(化肥...