安西县玉米茎基腐病的研究

甘肃省安西县玉米茎基腐病病原鉴定为小麦根腐平脐蠕孢Bipolaris sorokiniana(Sacc.) Shoemaker.玉米品种Sc704比中单二号感病:玉米小麦间作田病株率61.7％单播玉米3.4％。     

定向筛选提高棉花抗枯萎病的病理学机制

以病圃定向筛选抗枯萎病大幅度提高的棉花材料和原感病品种为对象,研究接种枯萎病菌Fusarium oxysporum f.sp.vasinfectum后抗、感材料的病理学变化。结果表明,二者在宏观病理学方面存在差异,感病品种接种7天开始显症,25天严重发病,整株维管束变色;抗病材料生长健康或只有轻微症状,维管束变色部位仅局限在子叶节以下。在细胞和组织病理学方面二者也存在差异,侵入前期,抗病材料被侵入细胞中有细胞壁加厚现象;侵入中期,病菌可通过表皮层侵入感病品种的薄壁组织,但只能到达抗病材料的皮层组织中;侵入后期,抗病材料中出现大量寄主细胞的降解物质或分泌物将菌丝包围,阻止病菌进一步扩展。抗病材料的抗侵入和抗扩展能力均较感病品种大幅度增强。     

五味子茎基腐病发生初报

通过对辽宁省多个地区的调查,发现此病害发生普遍,尤其是二年生的五味子茎基腐病发生严重。五味子茎基腐病在5月上旬开始出现,6月初为发生盛期。本文对五味子茎基腐病进行了症状描述,并对不同地点采集的病样分别进行木质部和韧皮部病原物的分离、纯化。对分离得到的菌株按照柯赫氏法则进行致病性测定并对病原菌进行鉴定,结果表明,此病害可由木贼镰刀菌(Fusarium equiseti)、茄腐镰刀菌(F.solani)、尖孢镰刀菌(F.oxysporum)和半裸镰刀菌(F.semitectum)4种镰刀菌属真菌引起。针对此病害的发生特点,提出了相应的防治措施。     

玉米内生菌L10的分离、鉴定及拮抗活性

为获得对玉米茎腐病主要病原菌禾谷镰孢Fusarium graminearum有明显拮抗作用的玉米内生菌,采用平板对峙法从成熟健康玉米茎秆中筛选禾谷镰孢拮抗菌株,并分析其抗菌谱;通过形态特征、生理生化特性及16S rDNA序列分析进行菌种鉴定;利用盆栽生防试验检测其对玉米茎腐病的防治效果。结果表明,共分离获得了164株玉米内生细菌菌株,其中L10菌株对禾谷镰孢具有较好的抑制效果,抑菌圈半径达1.68 cm;该菌对玉米大斑病菌Setosphaeria turcica、层出镰孢F. proliferatum、禾谷镰孢F. graminearum、拟轮枝镰孢F. verticilliodes、玉米弯孢叶斑病菌Curvularia lunata、玉米小斑病菌Bipolaris maydis、立枯丝核菌Rhizoctonia solani、茄链格孢Alternaria solani共8种植物病原菌均有拮抗作用,尤其对禾谷镰孢抑制效果最佳;结合形态特征、生理生化性质及16S rDNA序列分析,将L10菌株鉴定为多粘类芽胞杆菌Paenibacillus polymyxa。L10菌株脂肽类物质对禾谷镰孢菌具有较好的抑制活性,且盆栽生防试验结果显示该菌株对玉米茎腐病具有一定的防治效果。表明菌株L10对玉米镰孢茎腐病的防治具有一定潜力。     

玉米穗、茎腐病镰孢菌相互关系的血清学研究

运用血清学方法研究了沈阳、河北、北京、吉林4个地区玉米穗、茎腐病镰孢菌在病原学上的相互关系。结果发现:各地区玉米穗、茎腐病串珠镰孢菌(Fusarium moniliforme)具有高度的同源性,亲缘关系近。各地区玉米穗、茎腐病禾谷镰孢菌(F.graminearum)的亲缘关系因地域不同而有差异。沈阳穗、茎腐病禾谷镰孢菌之间差异明显,存在生理分化现象;吉林玉米穗、茎腐病病原物存在较高的相似性,但也有一定的差异;而河北玉米穗、茎腐病禾谷镰孢菌具有高度的同源性。     

棉花组织结构与黄萎病抗性的关系

 黄萎病严重危害棉花生产。本试验以温室种植31 个不同抗性的棉花品种为材料,通过对供试品种的组织结构研究,使用根系扫描法和石蜡切片法,了解棉花对黄萎病的抗性机制。结果如下:棉花根系的吸收根根长密度与相对病指呈显著正相关,相关系数为0. 923;抗病品种与耐病品种、感病品种的总长度、总投影面积、总表面积和吸收根根长密度差异显著,但耐病品种与感病品种的这4 个指标差异不显著。抗病材料根、茎的组织中薄层细胞密度大于感病材料,耐病品种根、茎的薄层细胞密度介于两者之间;抗病品种的根和茎的导管数最多,导管直径最小,其次是耐病品种,感病品种的根和茎中导管数最少,但是导管直径最大。棉花根系的吸收根根长密度,根和茎的薄层细胞密度,导管数可以作为对黄萎病抗性的检测指标。     

我国南方稻区水稻骨干亲本纹枯病抗性鉴定与分析

运用改进的纹枯病菌接种法,对当前我国南方稻区17个骨干亲本纹枯病抗性进行了田间鉴定,测定和分析了各材料的纹枯病病级(R_D)、相对病斑高度(H_R)及株高(H_P)。通过试验,将17个骨干亲本分为抗病、中等抗病、中等感病和感病4种类型;筛选出了3个抗病亲本(明恢63、蜀恢527、广恢128)和3个中抗亲本(绵恢725、珍汕97B和协青早B);供试亲本的平均RD为4.78,平均HR为0.52;通过分析,发现纹枯病病级(R_D)和相对病斑高度(H_R)呈极显著正相关(y=8.373x+0.435,y=RD;x=H_R)、株高和病级相关不显著。     

青枯菌(Pseudomonas solanacearum)在番茄抗、感病品种根部的吸附、侵入和繁殖

 采用不同的方法接种和利用扫描、透射电镜观察,研究不同致病力的青枯菌对番茄抗病及感病品种根部的吸附、侵入与繁殖。发现番茄抗病品种与感病品种的植株体内在菌体数量上有明显差异,而与青枯菌对番茄根部的吸附关系不显著。电镜观察发现青枯菌强致病力菌株菌体能以游离的形式存在于番茄感病品种根部的细胞间隙中,并能降解植株细胞壁、破坏原生质膜;青枯菌强致病力菌株菌体在抗病品种根内和青枯菌强致病力菌株在抗病及感病品种根内均被番茄植株细胞壁吸附,并且被细胞壁周围的浓密物质所包围。     

东方百合对茎腐病的抗性分析

为探明东方百合茎腐病的发病机制及抗病机理,以湖南株洲地区的东方百合为材料,分别采用升汞消毒、刺伤浸苗接种、超声波甲醇浸提、混液平板等方法对其病原菌进行了分离、抗病性鉴定、皂苷含量及活性测定。结果显示：尖孢镰刀菌Fusarium oxysporum为湖南株洲地区东方百合茎腐病病原菌;30份自主培育的东方百合材料通过聚类分析可划分为3类,包含抗病材料3份、中抗材料16份、感病材料11份,其中y、ZN-12和ZN-27的抗病指数都在65.00以上;抗病材料的总皂苷含量明显高于感病材料,且皂苷含量与抗性水平之间极显著相关;总皂苷含量为1.194 mg/mL的提取液对茎腐病病原菌菌丝生长的抑制率为29.39%。表明y、ZN-12和ZN-27具有较好的开发潜力;总皂苷含量与东方百合抗茎腐病水平密切相关,可作为大规模筛选抗病材料的一个重要指标。     

青枯菌(Pseudomonas solanacearum)在番茄抗、感病品种根部的吸附、侵入和繁殖

采用不同的方法接种和利用扫描、透射电镜观察,研究不同致病力的青枯菌对番茄抗病及感病品种根部的吸附、侵入与繁殖。发现番茄抗病品种与感病品种的植株体内在菌体数量上有明显差异,而与青枯菌对番茄根部的吸附关系不显著。电镜观察发现青枯菌强致病力菌株菌体能以游离的形式存在于番茄感病品种根部的细胞间隙中,并能降解植株细胞壁、破坏原生质膜;青枯菌强致病力菌株菌体在抗病品种根内和青枯菌强致病力菌株在抗病及感病品种根内均被番茄植株细胞壁吸附,并且被细胞壁周围的浓密物质所包围。     

Microscopical studies of resistance to powdery mildew disease in the hop cultivar Wye Target

Microscopical studies of fungal development and host responses during infection of the hop cultivars Northern Brewer (susceptible) and Wye Target (resistant) with a Zenith isolate of Sphaerotheca humuli are described. Resistance to powdery mildew disease in cv. Wye Target is principally determined by the R₂ major gene. Fungal growth was typically restricted in cv. Wye Target following formation of a single haustorial initial or haustorium which failed to develop the characteristic lobes found in susceptible cells. The hypersensitive reaction of penetrated epidermal cells was associated with death of haustorial initials but the associated appressorium remained alive during the first 2 days after inoculation. In leaves expressing resistance, histochemical staining revealed deposition of lignin-like material and callose in penetrated cells and to a lesser extent in underlying palisade mesophyll cells. Transmission electron microscopy and enzymic digestion clearly demonstrated extensive paramural deposition of β-1.3 glucans (callose) in reacting mesophyll cells. Plant cell death, lignification and widespread callose deposition were rarely observed at infection sites in cv. Northern Brewer but collars of callose were deposited around the necks of all haustoria formed.     

Fine structure of phloematic trypanosomatid–coconut tree interaction

Phytomonas wilt or Hartrot is a fatal disease of palm (Arecaceae) species including Cocos nucifera (coconut) and is caused by a phloematic trypanosomatid, a promastigote parasite that inhabits phloem sieve elements of disease palms. In the present work, we described the morphology of the interaction between a phloematic trypanosomatid (Phytomonas staheli) and C. nucifera. Two varieties and one ecotype of the adult coconut palm from northeast and southeast Brazil were analyzed, totaling 34,000 plants. Coconut palm losses due to Hartrot varied according to the variety or ecotype and geographic area. Occurrence of Hartrot was insignificant in Rio de Janeiro state (southeast), but in Bahia state (northeast) losses were substantial when appropriate cultural practices were not applied. Symptomatic and healthy palm tissues were analyzed by light and electron microscopy. Laboratory diagnoses revealed the twisted promastigote form of phloematic trypanosomatids in the extracts of shoot apex, leaves, stems and inflorescence in diseased plants, but not in the healthy ones. No parasites were found in the roots. Although the general anatomy of healthy and diseased palms was similar, callose deposition in the sieve plates was revealed by histochemistry and immunocytochemistry in the diseased tissue. Plugging by the P-protein and plastid alterations was also observed. Our observations strongly suggest that parasite traffic between sieve elements took place, although their cell bodies were larger than the sieve pores. Phloematic trypanosomatid proliferation in the sieve tube elements might interrupt the transport of phloem or/and consume plant nutrients. In addition, an association between the percentage of sieve elements colonized by pathogen in palm tissues and disease severity was established.     

Basal tomato defences to Botrytis cinerea include abscisic acid-dependent callose formation

In Arabidopsis, abscisic acid (ABA) application can induce resistance by priming for callose deposition; this resistance is impaired in ABA-deficient and -insensitive mutants. In tomato, ABA-deficiency causes resistance to Botrytis cinerea. Here, we show that callose deposition after B. cinerea inoculation is weaker in the ABA-deficient sitiens tomato mutant compared to the wild type (WT). Inhibition of callose synthesis did not affect resistance in sitiens, but caused additional susceptibility in WT. These findings indicate that callose deposition is not part of sitiens defence responses that are effective in blocking B. cinerea and suggest that callose deposition only contributes to WT basal resistance. Furthermore, also in tomato callose formation is at least in part ABA-dependent. However, it seems that in contrast to Arabidopsis, basal ABA levels in tomato are sufficiently high to prime for callose deposition.     

小麦族6种根茎型牧草叶表皮气孔特性与抗旱性的研究

本文对小麦族 6种根茎型牧草叶的气孔特性与抗旱性的关系进行了研究 ,结果表明 :6种禾草间气孔特性存在着基因型的本质差异 ;赖草属中 ,赖草叶对水分的蒸腾、气体交换的调控功能强于羊草和大赖草 ,其抗旱性强 ;在偃麦草属中 ,中间偃麦草叶的水分的蒸腾、气体交换的调控功能弱于其他 2种 ;在同种不同品种中 ,巴顿硬叶偃麦草叶的抗旱性强于茹莎娜硬叶偃麦草 ;6种禾草中 ,赖草属植物抗旱性强于偃麦草属植物。     

Panama Disease: Cell Wall Reinforcement in Banana Roots in Response to Elicitors from Fusarium oxysporum f. sp. cubense Race Four

ABSTRACT The biochemical basis of tolerance in banana to Fusarium wilt, caused by the pathogen Fusarium oxysporum f. sp. cubense race four, was investigated. Tissue culture banana plants from tolerant cv. Goldfinger and susceptible cv. Williams were maintained in a hydroponic system and inoculated with conidial suspensions to evaluate the degree of tolerance to susceptibility between the two clones and to investigate the effectiveness of this technique as a potential tool for early screening for resistance in breeding programs. Similarly, defense responses were induced by treatment of the plants with an elicitor preparation from the mycelial cell walls of the pathogen. Differences in the induction of lignin and callose deposition, phenolics, and the enzymes involved in cell wall strengthening; phenylalanine ammonia lyase, cinnamyl alcohol dehydrogenase, peroxidase, and polyphenol oxidase were determined. Root tissue of the tolerant cv. Goldfinger responded to the fungal elicitor through the strong deposition of lignin, preceded by the induction or activation of the enzyme activities involved in the synthesis and polymerization thereof, whereas only slight increases were observed for the susceptible cv. Williams. No increase in callose content was observed for either clone. These results indicate an important role for cell wall strengthening due to the deposition of lignin as an inducible defense mechanism of banana roots against F. oxysporum f. sp. cubense race four.     

水稻叶鞘3种氧化还原酶活性在褐飞虱胁迫中的变化

为阐明水稻抗褐飞虱的机制,分析了褐飞虱为害对感、抗虫水稻品种叶鞘中3种保护酶活性的影响。采用苗期群体接虫方法对水稻品种进行抗性鉴定,在此基础上测定了褐飞虱为害前后水稻叶鞘中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性。受褐飞虱为害后,抗虫和感虫品种叶鞘中SOD活性均上升,表明无论是抗性植株还是感性植株在清除超氧阴离子自由基方面都具有较强的能力;POD活性在抗虫品种中下降,而在感虫品种中增加,表明抗性品种较感性品种具有更强的酶促抗氧化性能,从而对褐飞虱胁迫具有更强的适应能力。CAT活性在抗虫品种中增加,而在感虫品种中大幅下降,这与其对褐飞虱抗性弱密切相关。     

Priming as a Mechanism in Induced Systemic Resistance of Plants

Induced systemic resistance is a plant defence state that is associated with an enhanced ability – the so-called priming – to resist pathogen attack by stronger activation of cellular defence responses. So far, however, priming has not been widely appreciated when studying induced plant disease resistance. During the past several years, it has been demonstrated that pre-treatment of cultured parsley cells with inducers of systemic resistance, salicylic acid or a benzothiadiazole, leads to the direct activation of a set of defence-related genes and also primes the cells for stronger elicitation of another set of defence genes including those encoding phenylalanine ammonia-lyase. From these results, it was concluded that the resistance inducers have at least a dual role in plant defence-gene activation. When elucidating whether priming plays a role in induced systemic resistance of Arabidopsis, pre-treating plants with benzothiadiazole was found to augment the subsequent activation of phenylalanine ammonia-lyase genes by Pseudomonas infection, wounding and osmotic stress and also to enhance wound/osmotic stress-induced callose production. The augmentation of phenylalanine ammonia-lyase gene activation or/and callose deposition was not seen in the Arabidopsis non-expresser of pathogenesis-related genes1 mutant which is compromised in induced resistance, while it was present, without benzothiadiazole pre-treatment, in the constitutive expresser of pr genes1 and 5 mutants in which induced resistance is constitutive. Together these studies point to priming as an important cellular mechanism in induced systemic resistance of plants which requires the intact non-expresser of pathogenesis-related genes1 gene.     

象耳豆根结线虫对抗病番茄品种VFNT的致病性研究

 象耳豆根结线虫是近年在我国发现的一种扩散迅速、寄主广泛、致病性强的根结线虫。为探究象耳豆根结线虫的强致病性原因,本研究用象耳豆根结线虫和南方根结线虫分别接种抗线虫番茄品种VFNT和感病番茄品种Rutgers,30 d后象耳豆根结线虫对VFNT和Rutgers的累计侵入率分别为14.1%和19.2%、虫瘿率为62.5%和81.6%,而此时南方根结线虫累计侵入率为0%和18.8%、虫瘿率为0%和68.5%,象耳豆根结线虫的侵染力和致病力显著强于南方根结线虫;通过侵染抗病番茄品种VFNT的病理切片以及根尖侵染早期胼胝质、活性氧染色发现象耳豆根结线虫的侵染不会激活Mi-1基因诱导的特异性抗性,在侵染早期南方根结线虫引起的胼胝质沉积量在72 h最大,是象耳豆根结线虫的2.0倍;体外H₂O₂应激测试发现,H₂O₂浓度为10～80 μmol·L^-1象耳豆根结线虫对H₂O₂的耐受性显著强于南方根结线虫。象耳豆根结线虫具有强致病性的原因可能是:具有更强的侵染力和活性氧耐受力;侵染不会激活Mi-1基因诱导的特异性抗性且侵染引起的胼胝质的积累量较低。     

Cytological Characterization of Elicitin-Induced Protection in Tobacco Plants Infected by Phytophthora parasitica or Phytoplasma

ABSTRACT Elicitins, small proteins secreted by Phytophthora and Pythium spp., display the ability to induce plant resistance toward pathogens. Ultrastructural investigations of cryptogein-treated tobacco plants evidenced host defense responses such as (i) formation of a calcium pectate gel in intercellular spaces of parenchymas, (ii) impregnation of pectin by phenolic compounds in intercellular spaces of phloem bundles, and (iii) accumulation of phloem proteins (P proteins) in the lumen of leaf sieve elements. These cytological modifications lead to the enhancement of physical barriers that prevent pathogen ingress and restrict host tissue colonization when cryptogein-treated tobacco plants were challenged with the pathogen Phytophthora parasitica. Wall appositions also were observed at most sites of penetration of hyphae. Moreover, growing hyphae exhibited severe morphological damages, suggesting a modified toxic environment. The same induction of P proteins in mature sieve tubes of tobacco leaves was obtained with oligandrin treatment, another elicitin. Cryptogein or oligandrin treatment prevented symptom expression in phytoplasma-infected tobacco plants in contrast with nontreated tobacco plants. Moreover, P protein plugs and occlusion of pore sites by callose were evidenced in sieve elements of treated plants. Both these phloem modifications might prevent the in planta movement of phloem-restricted microorganisms.     

大麦品种对白粉病抗病性鉴定及抗源筛选

通过田间初筛和病圃复筛,对6515份国内外大麦品种进行了白粉病抗性鉴定。重点材料结合苗期、分蘖期鉴定和异地鉴定,筛选出免疫或高抗材料52份,慢白粉病抗性材料59份,并发现具有Mla、Mla6单基因和Mla Mlat、Mla Mlh Mlp多基因组合的国外已知抗病基因品种,在浙江舟山和福建莆田表现抗病。统计分析表明,二棱大麦抗病性较多棱大麦强;抗病品种发病较感病品种迟;抗性材料大多来自国外,英、德、瑞典、叙利亚、澳大利亚、墨西哥等国品种,筛出率较高。