Major Extractable Organic Compounds in the Biologically Degradable Fraction of Fresh,Composted and Anaerobically Digested Household Waste

The recirculation of plant nutrients from urban areas to agriculture and horticulture and a need to reduce waste disposal by landfilling and incineration are the main reasons for recycling the biologically degradable fraction of household waste. However, before using the recycled material in agricultural and horticultural production it is necessary to make sure that the material does not contain any unwanted contaminants. In general, there is a lack of knowledge about organic contaminants in the degradable fraction of household waste and its compost and anaerobic digestion products. As a first step in a study of organic contaminants in household waste, and as part of the characterization of the degradation processes during composting and anaerobic digestion, the major extractable organic compounds were identified in the biologically degradable fraction of fresh, composted and anaerobically digested household waste. The organic compounds were isolated by supercritical fluid extraction using carbon dioxide as extraction medium. Gas chromatography-mass spectrometry was used for qualitative analyses. Natural products, such as fatty acids, fatty acid esters, n-alkanes, aliphatic alcohols, monoterpenes and triterpenes constituted the major organic components in the waste samples. In addition bis-(2-ethylhexyl) phthalate, frequently used as a plasticizer in polymers, was found in the extracts. In the chromatograms of the extracts of the fresh and composted household waste, fatty acids and fatty acid esters dominated with respect to peak heights and the number of peaks identified. In the anaerobically digested household waste n-alkanes were the dominating compound class. The pattern of the n-alkanes, also found in the fresh waste, possibly indicates a petrogenic origin of these compounds, perhaps caused by contamination during the collection of the household waste.     

Utilizing Composted Beef Cattle Manure and Slaughterhouse Waste as Nitrogen and Phosphorus Fertilizers for Calcareous Soil

Effectively utilizing composts requires that their nitrogen (N) and phosphorus (P) contents be used as fertilizer, but how this is best accomplished is not fully understood. The authors' objective was to quantify N and P availability of a calcareous clay loam soil receiving composts derived from four contrasting beef cattle feedlot feedstocks applied at 50, 150, and 450 mg total P kg^?1 and supplemented twice with fertilizer N for a 42-week greenhouse plant bioassay. Three composted manures from beef cattle fed distinct diets and a composted mix of slaughterhouse and construction waste were applied. Inorganically fertilized and non-amended soils were included as controls. Canola (Brassica napus L.) and pea (Pisum sativum L.) were grown in pots containing 1.5 kg air-dried soil for six alternating 7-week cycles. Soils amended with composted manure from beef cattle fed typical finishing diets had the lowest apparent N recovery (31%) and the greatest soil nitrate after 42 weeks (25 mg N kg^?1). Phosphorus availability was greater with composted manure from beef cattle fed distillers' dried grains than composted manure from beef cattle fed typical finishing diets and a composted mixture of slaughterhouse and construction waste. Apparent P recovery (66%) was greatest from composted manure of beef cattle fed corn (Zea mays L.) distillers' dried grains applied at 50 mg total P kg^?1. Composted manure from beef cattle fed distillers' dried grains had greater P availability than conventional composted beef cattle feedlot manure. Overall, performance of the composted mixture of slaughterhouse and construction waste was similar to the composted beef cattle manures.     

Ryegrass Utilization of Nutrients Released from Composted Biosolids and Cow Manure

Use of composts as soil amendments to enhance crop growth requires a knowledge of rates and amounts of nutrients released. A greenhouse study was conducted using ryegrass (Lolium perenne L.) as a test plant to evaluate this release from composts. The experimental design consisted of four blocked replicates in a complete factorial with two types of compost (wastewater treatment plant biosolids and cow manure), four application rates (1, 2, 5 and 10 percent of weight of sand), and three fertilizer treatments (0, 100 mg N/kg mixture, and 100 mg P/kg mixture). Rye-grass top growth was harvested after 21 days. The regrowth was harvested three additional times. Roots were recovered after the fourth harvest. Total N uptake was significantly and positively affected by the total amount of N supplied by the compost or compost plus N fertilizer (r² values ranged from 0.992 to 0.999). Initial N uptake depended on the mineral N concentration in the compost and was higher from biosolids than from cow manure compost. Biosolids compost contained 10 times more mineral N and this N was primarily taken up in the first two harvests. Cow manure compost, however, provided N gradually over the entire 84 day test. In addition to N, both composts also supplied P, K, and other major and minor nutrients essential for plant growth.     

Predicting Nitrogen and Carbon Mineralization of Composted Manure and Sewage Sludge in Soil

The capability of organic wastes to release available N in soil varies largely, depending on their source and form of production, or rather on their composition and biodegradability. Our purpose was to predict mineralization rates of different materials using their analyses joined with a simulation model, and to evaluate the influence of soil type and application rate of the organic materials on N and C transformations in soil. Four organic materials, sewage sludge (SS), sewage sludge compost (SSC), cattle manure compost (CMC), hen and cattle manure compost (HCMC), were applied to two soils at rates of 2 and/or 4%. The soils were incubated aerobically for 168 days at 30°C, during which CO₂ evolution rates and mineral-N concentrations were measured periodically. Hot water extractable C and N of all organic amendments correlated well with short term C and N mineralization, except HCMC that immobilized N although its soluble N content was large. NCSOIL, a computer model that simulates C and N cycling in soil with organic amendments, predicted well C and N mineralization of SS, SSC and CMC when considered as three-pool materials that decomposed at specific rates of 0.4, 0.024 and 10^?4 d^?1, using hot water soluble C and N as the labile pool. N immobilization by HCMC could be simulated only if the distribution of N between the labile and resistant pools was derived by optimization of NCSOIL, while hot water soluble C was labile. Laboratory methods to determine an intermediate pool or components that contribute to immobilization are required for improving the predictions of C and N mineralization from organic amendments.     

Soil-Applied Nitrogen and Composted Manure Effects on Soybean Hay Quality and Grain Yield

ABSTRACT

Grain yield in many soybean experiments fails to respond to fertilizer nitrogen (N). A few positive responses have been reported when soybean were grown in the southern U.S., when N was applied near flowering and when biosolids were added. In a previous study, low N concentrations of soybean forage in north Texas on a high pH calcareous soil were reported and thus, we suspected a N nutrition problem. Consequently, we initiated this study to determine whether selected preplant N sources broadcast and incorporated into a Houston Black clay (fine, smectitic, thermic Udic Haplusterts) might increase forage N concentration, forage yield, or soybean grain yield. In 2003, N was applied as ammonium nitrate (NH₄NO₃, AN) up to 112 kg N ha^{? 1} and dairy manure compost (DMC) was applied at rates of 4.9, 9.9, 15.0, and 19.9 Mg ha^{? 1}. The DMC contained 5.9, 2.6, and 6.7 g kg^{? 1} of total N, P, and K, respectively; thus DMC added 29 to 116 kg N ha^{? 1}. In 2004, AN was applied at rates of 112 and 224 kg N ha^{? 1} and DMC was applied at 28 and 57 Mg ha^{? 1}; thus, DMC added 168 to 335 kg N ha^{? 1}. In another 2004 test, biosolids, a biosolids/municipal yard waste compost mixture (BYWC), and AN were compared. The biosolids contained 31, 18, and 2.9 g kg^{? 1} total N, P, and K, respectively. The BYWC mixture contained 8.8, 6.1, and 3.4 g kg^{? 1} of total N, P, and K, respectively. Biosolids were applied at 10 Mg ha^{? 1} (310 kg N ha^{? 1}), BYWC was applied at 58 Mg ha^{? 1} (510 kg N ha^{? 1}), and AN up to 224 kg N ha^{? 1}. None of the soil treatments increased soybean grain yield or forage yield although AN slightly increased forage N concentration in 2003.     

牛粪中磷释放运移特征

采用取样分析和室内土柱模拟的方法,研究牛粪中磷在水、0.5mol/LNaHCO3和土体中的释放运移特点。研究结果表明,牛粪经H2O和NaHCO3分别提取后,H2O提取液中总磷(Pt)占全磷(TP)质量分数的31.54%,NaHCO3提取液中Pt占TP质量分数的64.74%;不同牛粪施用量处理土柱淋溶液中水溶性总磷(TDP)、水溶性无机磷(DRP)和水溶性有机磷(DOP)质量浓度均随着淋溶次数的增加呈先上升后降低的趋势;同一淋溶次数,随着牛粪施用量的增加,淋溶液中TDP、DRP和DOP质量浓度增加;在前6次淋溶液中,同一处理、同一次淋溶液中DOP质量浓度明显高于DRP质量浓度。土壤大量施用牛粪(30t/hm2以上),增加0-30cm土体中土壤全磷(TP)和0-60cm土体中土壤速效磷(Olsen-P)的质量分数。     

The Effects of Fresh and Composted Chicken Manures on Some Soil Characteristics

The effects of fresh and composted chicken manures on some soil characteristics were investigated. Fresh and composted chicken manures were mixed with the soil at the rates of 0%, 1%, 2%, 3%, and 4%, and then were incubated for 90 days. At the end of this incubation period, some soil physical properties closely related to soil aggregation (such as hydraulic conductivity, water stable aggregates, aeration porosity, and available water) and some soil chemical characteristics (such as organic carbon, total nitrogen, pH, and electrical conductivity, EC) were determined. As doses of both manures in the soil mixtures increase, the amounts of water stable aggregates, hydraulic conductivity, and aeration porosity increased, whereas the available water content decreased. Increasing doses of both manures increased the amount of the soil organic carbon and total nitrogen. Application of both manures to the soil increased the EC values, but it was limited in the composted chicken manure.     

Use of Composted and Fresh Spent Tea Grinds As a Potential Greenhouse Substrate Component

In the United States, the market for freshly brewed, ready-to-drink tea has grown exponentially during the last 20 years. Along with generating more product, tea brewers have also produced more waste. Spent tea grinds (STG) are the finely ground waste product of the tea brewing process. STG's high water-holding capacity and organic nature make it a potential replacement for common substrate components such as pine bark (PB) and peat moss (PM). In an experiment, Lantana camara L. 'New Gold' and Nephrolepis exaltata L. 'Bostoniensis', were grown in various substrates for 10 weeks on a raised bench in a greenhouse. Four substrates, which were 100% STG, 1:1 mixture of cocomposted PB:STG, Fafard® 3B, and a composted 1:1 mixture of PB:STG, were evaluated in this experiment. Substrates either contained a preplant incorporated, complete fertilizer ^(+F), or contained no exogenous fertilizer ^(?F). Lantanas grown in tree bark compost (TBC)^+F and a 1:1 mixture of PB:STG^+F had the greatest growth index at harvest, while STG^+F was not different than TBC^+F. Lantanas grown in TBC^+F, Fafard® 3B^+F, and a 1:1 mixture of PB:STG^+F had the highest shoot dry weights at harvest. No differences existed in growth index or shoot dry weight of boston fern. Substrate leachate pH was at the high end, or slightly above, a recommended range for the duration of the experiment for substrates containing TBC, but plant growth was not reduced.     

Cocomposting of Cattle Manure and Hydrocarbon Contaminated Flare Pit Soils

The potential of using composting technology to remediate clayey soils with high levels of crude oil contamination was evaluated. An open air windrow comprised of flare pit soil, manure and wood chips was constructed at Olds College, Composting Technology Centre. Aeration and mixing were carried out by a skid steer loader and composting parameters were monitored for ten months. Temperature profile of this windrow gave cyclic patterns of high and low temperature recordings corresponded to the turning events. Most of the microbial metabolic activity occurred within the mesophilic temperature range and the hydrocarbon degrading microorganism populations remained high throughout the trial. Complete removal of BTEX compounds was achieved within six months and extractable carbons from C₅ to C₁₀ were reduced by 98.7% compared to the initial contaminated soil. Vegetative growth on the composted soil was also evaluated. Barley and timothy plants grown in the composted soil were compared to the control off-lease soil, contaminated soil, and other treatments of varying salinity and organic matter levels. Plant germination, survival, and biomass production was significantly better in the composted soil than in the contaminated soil. Furthermore, barley plants grown in the composted soil were more resilient than those grown in the control off-lease soil.     

Nitrification and Denitrification after Direct Injection of Liquid Cattle Manure

Abstract

Soil cores were collected in and around an injection slit in each of two field plots on a coarse sandy soil. The plots received either raw or anaerobically digested liquid cattle manure at a rate of 240 kg NH₄ ⁺-N ha^?1. During the three week period of the experiment, concentrations of dissolved organic carbon and NH₄ ⁺ and the moisture content of cores from the injection slit were consistently above the background level in the soil. Denitrification activity was only registered in soil cores sampled in the injection slit. A dramatic increase occurred between Day 14 and Day 21, when the denitrification rate reached 3.5 kg N ha^?1day^?1 in cores from the plot treated with raw manure, while the rate was 20-fold lower in the plot treated with digested manure. Nitrate accumulated between Day 7 and Day 21, suggesting a coupling between nitrification and denitrification.     

规模化养牛场粪便处理生命周期评价

以某规模化养牛场为例,应用生命周期评价方法,对畜禽粪便两种不同处理方式进行生命周期污染物排放清单分析,在此基础上进行了生命周期环境影响评价。结果表明,畜禽粪便处理过程中主要的环境影响类型是全球变暖,其次是环境酸化和富营养化。其中好氧堆肥工艺的环境酸化和富营养化潜力大于厌氧发酵处理工艺,厌氧发酵工艺全球变暖潜力大于好氧堆肥。综合比较,厌氧发酵的环境影响优于好氧堆肥,其环境影响综合指数分别为0.011 2、0.024 3,该养殖场宜采用厌氧发酵工艺处理畜禽粪便。     

Changes in Soil pH and Extractable Phosphorus Following Application of Turkey Manure Incinerator Ash and Triple Superphosphate

During the incineration of turkey manure, a wide variety and concentration of nutrients are conserved in the turkey manure ash (TMA). In particular, the high concentration of citrate-soluble phosphorus (P) (43 g kg^?1) may make it a suitable P source for crop production. The ash is alkaline with a pH of 12.2. We conducted a soil incubation study using a low-P soil with a pH of 6.2 to evaluate the effects of TMA on soil pH and extractable P. Two TMA rates, based on citrate-soluble P (10.9 and 21.9 mg kg^?1), were compared with equivalent rates of triple superphosphate (TSP). In addition, a 0-P control was included. At the rates tested, TMA slightly increased soil pH, but this increase would be of minor agronomic importance. At equivalent P rates, changes in water-soluble P (WSP) concentrations with TMA and TSP were similar. Changes in iron-oxide-strip–extractable P (FeO P) and Bray 1 P concentrations were greater with TMA than with TSP. In contrast, changes in Olsen P concentrations were greater with TSP than with TMA for up to 32 days. For TMA, the ability of the tests to extract P was ranked, from highest to lowest, as Bray 1 P > FeO P > Olsen P > WSP, and for TSP they were ranked Bray 1 P ≥ Olsen P ≥ FeO P > WSP. However, the Bray 1 P and FeO P tests tended to overestimate plant-available P because they measured more citrate-soluble P than that added with TMA. We conclude that TMA can be used as a P source for crop production and that the Olsen test may provide a better estimate of plant-available P in TMA-amended soil.     

Surface Soil Salinity and Soluble Salts after 15 Applications of Composted or Stockpiled Manure with Straw or Wood-Chips

The influence of 15 annual applications of composted (CM) or stockpiled (SM) beef feedlot manure with straw (ST) or wood-chip (WD) bedding on electrical conductivity (EC), soluble cations and anions (Na, K, Ca, Mg, SO₄-S, Cl), sodium adsorption ratio (SAR), potassium adsorption ratio (PAR), and pH of a clay loam soil (0–15 cm) in southern Alberta was examined in an irrigated barley silage cropping system. Manure type (CM versus SM) had a significant effect on certain soil salinity parameters. Calcium, Mg, Na, K, and SO₄-S were significantly (p ≤ 0.05) greater for SM- than CM-amended soils for certain bedding materials and rates. Electrical conductivity, concentration of soluble cations and anions (Na, K, SO₄-S, Cl), SAR, PAR, and pH in the surface soil were greater for ST than WD bedding. Two exceptions were Ca and Mg, where soil concentrations were generally greater for WD than ST. Salinity parameters were greater with increased application rate, and greater for amended than unamended soils. Overall, bedding had considerably more significant effects on soil salinity parameters compared to manure type. Wood-chip bedding may be a management tool for feedlots to lower EC, soluble cations and anions, and pH of surface soils.     

Nutrient Uptake and Leaching from Soil Amended with Cattle Manure and Nitrapyrin

We investigated whether nitrification inhibitor nitrapyrin can reduce nutrient leaching and increase nutrient uptake by corn (Zea mays L.) in cattle manure amended soil. Amendments included non-amended check (CK), urea (Urea), REG (manure from cattle fed barley grain), and DDGS (manure from cattle fed 60% dried distillers grains with solubles), co-applied with or without nitrapyrin and leached or unleached with water. Nitrapyrin reduced (P < 0.01) leaching of nitrate by 56, 32, and 24% from DDGS, REG, and Urea treatments, respectively, and also reduced (P < 0.05) leaching of phosphate (58%), potassium (39%), calcium (39%), and magnesium (39%) from DDGS treatment. While nitrapyrin reduces the rate of ammonium conversion to nitrate, higher magnesium and phosphate levels in DDGS-amended soil favor struvite formation and reduce their leaching. Corn biomass and nutrient uptake were higher (P < 0.01) in DDGS and Urea than CK and REG treatments, but remained unaffected by nitrapyrin. The benefits of nitrapyrin should be further investigated under field conditions.

Abbreviations: DCD, dicyandiamide; DDGS, dried distillers grains with solubles; NI, nitrification inhibitor; TP, total P; TN, total N.     

沼液堆肥化与牛粪堆肥化的发酵特性及腐熟进程

为评价沼液作为堆肥含氮添加剂的应用效果,开发沼液的处理应用技术,以牛粪树叶堆肥为对照,将沼液和树叶混合堆制发酵,探讨其发酵特性与腐熟进程。研究结果表明,环境温度一直在10℃以下,沼液堆肥化和牛粪堆肥化均能经历35d以上的堆温超过50℃的高温发酵;而沼液堆肥化超过50℃的高温期持续时间比牛粪堆肥化少8d;经60d的发酵沼液堆肥化的半纤维素含量从发酵初期的12.14%下降到6.53%,纤维素含量由20.5%下降到9.8%;而牛粪堆肥化的半纤维素含量从12.8%下降到9.56%,纤维素含量由21.5%下降到15.9%。可见沼液堆肥化的分解更彻底。从C/N、温度、可溶性糖含量、含水量、种子发芽指数综合评价两种堆肥的腐熟度,沼液堆肥化进入腐熟状态约经30d,而牛粪堆肥化进入腐熟约需45d。     

Lime and Cattle Manure in Soil Fertility and Soybean Grain Yield Cultivated in Tropical Soil

Most tropical soils have high acidity and low natural fertility. The appropriate application of lime and cattle manure corrects acidity, improves physical and biological properties, increases soil fertility, and reduces the use of chemical and/or synthetic fertilizers by crops, such as soybean, the main agricultural export product of Brazil. This study aimed to assess the effects of the combination of the application of dolomite limestone (0, 5, and 10 Mg ha^?1) and cattle manure (0, 40, and 80 Mg ha^?1) on grain yield and the chemical properties of an Oxisol (Red Latosol) cultivated with soybean for two consecutive years. The maximum grain yield was obtained with the application of 10 Mg ha^?1 of lime and 80 Mg ha^?1 of cattle manure. Liming significantly increased pH index, the concentrations of calcium (Ca²⁺) and exchangeable magnesium (Mg²⁺), and cation exchange capacity (CEC) of soil and reduced potential acidity (H⁺ + Al³⁺), while the application of cattle manure increased pH level; the concentrations of potassium (K⁺), Ca²⁺, and exchangeable Mg²⁺; and CEC of the soil. During the 2 years of assessment, the greatest grain yields were obtained with saturation of K⁺, Ca²⁺, and Mg²⁺ in CEC at the 4.4, 40.4, and 17.5 levels, respectively. The results indicated that the ratios of soil exchangeable Ca/Mg, Ca/K, K/Mg, and K/(Ca+Mg) can be modified to increase the yield of soybean grains.     

牛粪沼渣中多重耐药细菌的环境风险

为研究牛粪厌氧发酵沼渣中多重耐药细菌的环境污染风险,选用畜禽养殖中常用的5种抗生素(红霉素、氨苄青霉素、金霉素、链霉素、环丙沙星)对牛粪沼渣中可培养抗生素抗性细菌进行筛选。结果表明：多重耐药细菌的比例高达76.5%,其中,抗5种和4种抗生素的细菌分别有11,21株。所有多重耐药细菌均对氨苄青霉素具有抗性,抗红霉素、金霉素和链霉素的细菌分别占总多重耐药细菌数的92.0%,89.3%,61.3%。通过细菌16S rRNA测序鉴定,32株具有4种以上抗性的细菌分别属于福氏志贺氏菌、摩根氏菌和假中间苍白杆菌,均为重要的临床致病菌。使用全基因组测序对7株典型多重耐药细菌携带的抗性基因进行分析,共检测出28种抗生素抗性基因,对应9种抗生素抗性类型。通过分析抗性基因及插入序列所在位点信息发现,多重耐药细菌普遍携带含有抗性基因和插入序列共存的质粒,表明抗性基因具有高度的可移动性和较强的传播风险。综上所述,牛粪沼渣中含有大量的多重耐药细菌,可视作抗生素抗性基因的储存库,应重视沼渣农业资源化利用过程中的环境风险监测与评估。     

牛粪对杨树人工林土壤生物学性状及其生长的影响

[目的]探讨施用牛粪对杨树人工林土壤生物学性状与生长的作用效果,为土壤培肥制度的建立及杨树人工林的合理施肥提供理论依据。[方法]通过3a大田试验,研究CK(对照,不施肥)、CF(单施化肥)、CM(牛粪)和CM+CF(牛粪和化肥各提供50%的氮)等处理对杨树人工林土壤理化特征、微生物数量、微生物量碳氮、活性有机碳、碳库管理指数(CPMI)及生长的影响。[结果]与CF处理相比,CM+CF处理显著提高了土壤中细菌数、真菌数、微生物总量和微生物量碳、氮含量,其中微生物量碳、氮含量分别较CF处理显著提高42.20%和43.75%;CM+CF处理还能明显提高土壤中碱解氮、速效磷、速效钾和交换性Mg含量,并显著降低土壤pH值。同时,CM处理使土壤总有机碳含量达最高值,而CM+CF处理使土壤的低活性、中活性、高活性有机碳含量和CPMI最高,其中CPMI分别较CK,CF和CM处理显著高出52.76,47.16,25.89。此外,CM+CF处理使杨树人工林的材积平均生长率达到45.36%,并显著高于其他处理。与CM+CF处理相比,CM处理对土壤和材积的影响效果较小。[结论]牛粪与化肥配施有助于改善杨树人工林土壤的生物学性状,有利于促进林木生长。     

Accumulations of Cadmium,Zinc, and Copper by Rice Plants Grown on Soils Amended with Composted Pig Manure

A pot experiment, in which composted pig manure was applied to soils at rates of 0%, 0.5%, 1.5%, 3.0%, and 5.0% (W/W) to simulate additions of different amounts of cadmium (Cd), copper (Cu), and zinc (Zn) to soil, was conducted to assess accumulation of metals by rice (Oryza sativa L.) plants from soils treated with manure. Results indicated that Cd concentrations in rice grains were more than the limit of 0.2 mg kg^?1 when 0.14 mg kg^?1 or more Cd was loaded to Ferralsols by manure application, but it was not more than the limit in Calcaric Cambisols. Zinc contents in polished rice grains did not exceed the permissible limit of 50 mg kg^?1 in two soils. Copper concentrations in rice grain were slightly more than the limit of 10 mg kg^?1 in Ferralsols but not in Calcaric Cambisols. Results suggested greater risk of heavy‐metal contamination from manure to paddy rice in Ferralsols than in Calcaric Cambisols.     

Yield Response of Corn to Single and Combined Application of Cattle Manure and Urea

A 2-year field experiment was conducted to evaluate the single- and combined-application effects of cattle manure and urea on corn (Zea mays L.) production. A randomized complete block design was conducted with five nitrogen (N) rates (36, 72, 108, 144, and 180 kg N ha^?1) as urea, cattle manure, or both. The stover yield and aboveground biomass increased with urea application up to 144 kg N ha^?1 but remained unchanged at greater N rates. At all N rates, combined application of manure and urea resulted in greater grain yields than single applications. Crop response to applied N was greater in the combined N application system than in the single-application treatments. The greatest grain yield was found in plots that had received a combination of 18 ton manure ha^?1 plus 160 kg urea ha^?1. Manure application along with urea enhanced crop yield response to urea and reduced its application rate.