不同施肥管理对红壤性水稻土有机碳、氮形态的影响

A long-term experiment beginning in 1981 in Jinxian County of Jiangxi Province, subtropical China, was conducted in a paddy field under a double rice cropping system with four different fertilization regimes, including 1) no fertilizer as control (CK), 2) balanced chemical N, P, and K fertilizers (NPK), 3) organic manure using milk vetch and pig manure in the early and late rice growing season, respectively (OM), and 4) balanced chemical fertilizers combined with organic manure (NPKM). Samples (0-17 cm) of the paddy field soil, which was derived from Quaternary red clay, were collected after the late rice harvest in November 2003 for determination of total organic carbon (TOC) and total nitrogen (TN) and fractions of organic C and N. Results showed that TOC and TN in the NPKM and OM treatments were significantly higher than those in other two treatments (CK and NPK). Application of organic manure with or without chemical fertilizers significantly increased the contents of all fractions of organic C and N, whereas chemical fertilizer application only increased the contents of occluded particulate organic C (oPOC) and amino acid N. In addition, application of organic manure significantly enhanced the proportions of free particulate organic carbon (fPOC) and oPOC in total C, and those of amino sugar N and amino acid N (P < 0.01) in total N. In contrast, chemical fertilizer application only increased the proportions of oPOC and amino acid N (P < 0.05). There were no significant differences in either contents or proportions of soil organic C and organic N fractions between the NPKM and OM treatments. These indicated that organic manure application with or without chemical fertilizers played the most significant role in enhancing soil organic C and N quantity and quality in the paddy field studied.     

除草剂和土壤改良剂对热带农业生态系统中微生物生物量动态的影响

The influences of herbicide alone and in combination with the soil amendments with contrasting resource qualities on dynamics of soil microbial biomass C (MBC), N (MBN), and P (MBP) were studied through two annual cycles in rice-wheat-summer fallow crop sequence in a tropical dryland agroecosystem. The experiment included application of herbicide (butachlor) alone or in combination with various soil amendments having equivalent amount of N in the forms of chemical fertilizer, wheat straw, Sesbania aculeata, and farm yard manure (FYM). Soil microbial biomass showed distinct temporal variations in both crop cycles, decreased from vegetative to grain-forming stage, and then increased to maximum at crop maturity stage. Soil MBC was the highest in herbicide + Sesbania aculeata treatment followed by herbicide + FYM, herbicide + wheat straw, herbicide + chemical fertilizer, and herbicide alone treatments in decreasing order during the rice-growing period. During wheat-growing period and summer fallow, soil MBC attained maximum for herbicide + wheat straw treatment whereas herbicide + FYM, herbicide + Sesbania, and herbicide + chemical fertilizer treatments showed similar levels. The overall trend of soil MBN was similar to those of soil MBC and MBP except that soil MBN was higher in herbicide + chemical fertilizer treatment over the herbicide + wheat straw treatment during rice-growing period. In spite of the addition of equivalent amount of N through exogenous soil amendments in combination with the herbicide, soil microbial biomass responded differentially to the treatments. The resource quality of the amendments had more pronounced impact on the dynamics of soil microbial biomass, which may have implications for long-term sustainability of rainfed agroecosystems in dry tropics.     

长期施肥对潮土耕层土壤和作物籽粒微量元素动态的影响

Micronutrient status in soils can be affected by long-term fertilization and intensive cropping.A 19-year experiment (1990-2008) was carried out to investigate the influence of different fertilization regimes on micronutrients in an Aquic Inceptisol and maize and wheat grains in Zhengzhou,China.The results showed that soil total Cu and Zn markedly declined after 19 years with application of N fertilizer alone.Soil total Fe and Mn were significantly increased mainly due to atmospheric deposition.Applications...     

长期施用化肥和有机肥对太湖地区水稻土有机碳特征的影响

A long-term experiment set up in 1980 compared the effects of applying manures and chemical fertilizers on a paddy soil in the Taihu Lake region,China.Of the fourteen randomly distributed treatments consisting of different combinations of organic manure,inorganic nitrogen (N),phosphorus (P),and potassium (K),and rice straw,eight were selected for the present study in 2007.Application of organic manure plus straw significantly increased soil organic carbon (SOC) content of the topsoil (0-10 cm) compared to that of chemical fertilizers alone.The content of SOC was relatively stable in the 10-30 cm layer in the chemical fertilizer treatments and in the 20-40 cm layer in the manure treatments.The stable carbon isotope ratio (δ 13 C) ranged from 24‰ to 28‰ and increased gradually with depth.The content of SOC was significantly (P < 0.05) negatively correlated with δ 13 C.In the 0-20 cm layer,the δ 13 C value significantly decreased in the treatments of manure alone (M),manure and chemical N and P fertilizers (MNP),manure and chemical N,P,and K fertilizers (MNPK),manure,rice straw,and chemical N fertilizer (MRN),and chemical N fertilizer and rice straw (CNR),as compared with the no-fertilizer control.In the 30-50 cm layer,however,the ratio significantly increased in all the treatments except Treatment CNR.Mineralization of organic C peaked in the first 2-4 d of incubation and gradually leveled off thereafter over the first 3 weeks,being faster in the manure treatments than the chemical fertilizer treatments.The average rate of mineralization varied from 55.36 to 75.46 mL CO 2 kg-1 d-1 and that of stable mineralization from 10 to 20 mL CO 2 kg-1 d-1.In eight weeks of incubation,cumulative mineralization was always higher in the manure treatments than the chemical fertilizer treatments,being the highest in Treatment MRN.Combined humus in the soil was mainly (over 50%) composed of tightly combined fraction.The loosely combined humus and its ratio of humic acid (HA) to fulvic acid (FA) significantly increased with long-term application of organic manure and chemical fertilizers.It could be concluded that the cycle of organic C in the paddy soil ecosystem studied was stable over the long-term application of fertilizers and continued cultivation.     

北美中部大平原半干旱农业生态系统中覆盖作物和灌溉对土壤微生物群落和酶活性的影响

Cover crops can have beneficial effects on soil microbiology by increasing carbon (C) supply, but these beneficial effects can be modulated by precipitation conditions. The objective of this study was to compare a fallow-winter wheat (Triticum aestivum L.) rotation to several cover crop-winter wheat rotations under rainfed and irrigated conditions in the semiarid US High Plains. Experiments were carried out at two sites, Sidney in Nebraska and Akron in Colorado, USA, with three times of soil sampling in 2012--2013 at cover crop termination, wheat planting, and wheat maturity. The experiments included four single-species cover crops, a 10-species mixture, and a fallow treatment. The variables measured were soil C and nitrogen (N), soil community structure by fatty acid methyl ester (FAME) profiles, and soil β-glucosidase, β-glucosaminidase, and phosphodiesterase activities. The fallow treatment, devoid of living plants, reduced the concentrations of most FAMEs at cover crop termination. The total FAME concentration was correlated with cover crop biomass (R = 0.62 at Sidney and 0.44 at Akron). By the time of wheat planting, there was a beneficial effect of irrigation, which caused an increase in mycorrhizal and protozoan markers. At wheat maturity, the cover crop and irrigation effects on soil FAMEs had subsided, but irrigation had a positive effect on the β-glucosidase and phosphodiesterase activities at Akron, which was the drier of the two sites. Cover crops and irrigation were slow to impact soil C concentration. Our results show that cover crops had a short-lived effect on soil microbial communities in semiarid wheat-based rotations and irrigation could enhance soil enzyme activity. In the semiarid environment, longer time spans may have been needed to see beneficial effects of cover crops on soil microbial community structure, soil enzyme activities, and soil C sequestration.     

长期施肥对土壤有机碳和无机碳的影响

利用18年长期定位试验,研究了在不同施肥条件下,土壤有机碳和无机碳在0~50 cm土层分布特征。结果表明,施肥对土壤有机碳的影响随着土层深度的增加而下降,0~7.5 cm土层的土壤有机碳比7.5~15 cm、15~30 cm、30~50 cm分别增加了4.6%、22.0%、63.1%,而无机碳含量随着土层深度的增加而增加,与有机碳的变化规律正好相反。不同种类的肥料对土壤有机碳的影响也不相同,化肥、有机肥长期配合施用和长期施用有机肥可以在0~30 cm土层增加土壤有机碳含量,降低土壤中的无机碳含量,而长期单施化肥对土壤的有机碳和无机碳含量无明显差异。     

长期施肥对土壤中硒的累积及其生物有效性的影响

Continuous applications of organic and inorganic fertilizers can affect soil and food quality with respect to selenium(Se) concentrations.A long-term(over 20 years) experimental field study,started in 1989,was conducted to investigate the changes in soil Se fractions and its uptake by crops,as affected by different fertilizer practices,in the North China Plain with an annual crop rotation of winter wheat and summer maize.The long-term experiment was arranged in a complete randomized block design consisting of 4replications with 7 fertilizer treatments:1) organic compost(OC),2) half organic compost plus half N-P-K chemical fertilizers(OC+ NPK),3) N-P-K fertilizers(NPK),4) N-P fertilizers(NP),5) P-K fertilizers(PK),6) N-K fertilizers(NK),and 7) an un-amended control.Soil samples from the surface(20 cm) were collected in 1989,1994,1999,2004 and 2009 to characterize Se and other soil properties.In 2009,the average soil Se concentrations in the treatments(149 ± 8 μg kg~(-1)) were higher than those in the soil samples collected in 1989 at the beginning of the experiment(112 ± 4 μg kg~(-1)),and decreased in the order of OC OC + NPK NPK≈NP PK ≈NK control.Sequential extraction showed the oxidizable fraction(50.06%± 3.94%) was the dominant form of Se in the soil,followed by the residual fraction(24.12%± 2.89%),exchangeable fraction(15.09%± 4.34%) and Fe-Mn oxides fraction(10.73%± 4.04%).With an increase of soil K,the exchangeable Se concentrations in the soil increased.The Se concentrations in the soil tillage layer(0-20 cm) were mainly related to soil organic carbon(SOC),although different contributions came from atmospheric deposition,irrigation and fertilizers.With the accumulation of SOC,the uptakes of soil Se by two crops were inhibited.For the OC and OC + NPK treatments,Se concentrations in wheat grains were lower than the critical standard of Se in stable food(100 μg kg~(-1)).Additionally,Se concentrations in grains were also decreased by the deficiencies of major soil nutrients,especially P.     

长期日光温室试验条件下不同施肥处理对土壤团聚体稳定性和铁铝氧化物含量的影响

Soil in greenhouses is likely to suffer a gradual decline in aggregate stability. Determination of the effects of different fertiliser practices on soil aggregate stability is important for taking advantage of solar greenhouses. Soil aggregate stability and iron(Fe) and aluminium(Al) oxide contents were investigated in a 26-year long-term fertilisation experiment in greenhouse in Shenyang, China,under eight fertiliser treatments: manure(M), fertiliser N(FN), fertiliser N with manure(MN), fertiliser P(FP), fertiliser P with manure(MP), fertiliser NP(FNP), fertiliser NP with manure(MNP), and control without any fertiliser(CK). A wet sieving method was used to determine aggregate size distribution and water-stable aggregates(WSA), mean weight diameter and geometric mean diameter as the indices of soil aggregate stability. Different fertiliser treatments had a statistically significant influence on aggregate stability and Fe and Al oxide contents. Long-term application of inorganic fertilisers had no obvious effects on the mass proportion of aggregates. By contrast, manure application significantly increased the mass proportion of macroaggregates at the expense of microaggregates. All treatments, with the exception of FNP, significantly increased the stability of macroaggregates but decreased that of microaggregates when compared with CK. Aggregation under MP and MN was better than that under M and MNP; however,no significant differences were found among inorganic fertiliser treatments(i.e., FN, FP, and FNP). A positive relation was found between pyrophosphate-extractable Fe and WSA(r = 0.269), but no significant relations were observed between other Fe and Al oxides and aggregate stability.     

中国黄土高原区轮耕对土壤团聚体、有机碳氮含量的影响

In rain-fed semi-arid agroecosystems, continuous conventional tillage can cause serious problems in soil quality and crop production, whereas rotational tillage (no-tillage and subsoiling) could decrease soil bulk density, and increase soil aggregates and organic carbon in the 0-40 cm soil layer. A 3-year field study was conducted to determine the effect of tillage practices on soil organic carbon (SOC), total nitrogen (TN), water-stable aggregate size distribution and aggregate C and N sequestration from 0 to 40 cm soil in semi-arid areas of southern Ningxia. Three tillage treatments were tested: no-tillage in year 1, subsoiling in year 2, and no-tillage in year 3 (NT-ST-NT); subsoiling in year 1, no-tillage in year 2, and subsoiling in year 3 (ST-NT-ST); and conventional tillage over years 1-3 (CT). Mean values of soil bulk density in 0-40 cm under NT-ST-NT and ST-NT-ST were significantly decreased by 3.3% and 6.5%, respectively, compared with CT, while soil total porosity was greatly improved. Rotational tillage increased SOC, TN, and water-stable aggregates in the 0-40 cm soil, with the greatest effect under ST-NT-ST. In 0-20 and 20-40 cm soils, the tillage effect was confined to the 2-0.25 mm size fraction of soil aggregates, and rotational tillage treatments obtained significantly higher SOC and TN contents than conventional tillage. No significant differences were detected in SOC and TN contents in the >2 mm and <0.25 mm aggregates among all treatments. In conclusion, rotational tillage practices could significantly increase SOC and TN levels, due to a fundamental change in soil structure, and maintain agroecosystem sustainability in the Loess Plateau area of China.     

长期施肥和秸秆还田对设施蔬菜土壤有机碳的影响

为了明确施肥和秸秆还田等措施对华北地区设施蔬菜土壤有机碳的影响,该研究以寿光设施蔬菜生产为对象,进行了连续11 a的长期定位试验,设置了对照（CK）、有机肥+秸秆还田（MS）、有机肥+优化氮肥（N1M）、有机肥+常规氮肥（N2M）、有机肥+优化氮肥+秸秆还田（N1MS）、有机肥+常规氮肥+秸秆还田（N2MS）6个处理,通过测定分析土壤有机碳含量、储量、有机物料碳转化效率、固碳速率等,探讨不同施肥和秸秆还田措施下土壤有机碳的变化规律。结果表明,施加有机肥+优化氮肥条件下,N1MS处理可以提高＞30～60 cm土壤有机碳含量,比N1M处理显著提高了40.5%（P＜0.05）,而施加有机肥+常规氮肥条件下,施加秸秆的增碳效果不明显;N2M显著增加表层（0～30 cm）土壤有机碳含量（P＜0.05）,比N1M处理提升47.0%;在有机肥+秸秆还田条件下,0～30 cm土层土壤有机碳随着施氮量的增加而增加,增加量大小顺序为N2MS（106.5%）＞N1MS（64.2%）＞MS（39.9%）;N2MS的土壤固碳速率在0～30和0～90 cm土层分别为1.85和3.74 t/(hm2?a)。因此,只考虑表层（0～30 cm）土壤固碳是远远不够的,应重视设施蔬菜等集约农业生产方式的深层土壤固碳。施用氮肥有利于提高有机物料转换为土壤有机碳的效率,且随着氮肥量的增加,有机物料的碳转化效率明显提高。设施蔬菜生产中,由于有机肥投入水平较高,秸秆还田转化为土壤有机碳的效率反而降低,秸秆还田和氮肥之间并未显现出对土壤有机碳的正交互作用,应进一步优化肥料和秸秆投入方式和水平,在维持蔬菜高产的前提下提高资源利用效率。     

添加牛粪对长期不同施肥潮土有机碳矿化的影响及激发效应

为了探讨长期不同施肥潮土有机碳矿化对添加牛粪的响应特征及添加牛粪对长期不同施肥潮土有机碳矿化的激发效应,以始建于1986年的长期定位试验为平台,通过室内恒温培养的方法研究添加等氮量牛粪后长期不同施肥(不施肥,CK;常量有机肥,SMA;常量化肥,SMF;常量有机无机配施,1/2(SMA+SMF))潮土有机碳矿化、土壤有机碳及活性碳库组分(微生物量碳、可溶性有机碳、颗粒有机碳和易氧化有机碳)含量的变化特征。结果表明:无论添加牛粪与否,长期不同施肥潮土有机碳矿化过程均符合一级动力学方程,而牛粪的添加显著增加了长期不施肥、长期单施化肥和长期有机无机配施土壤的有机碳矿化速率常数,增长幅度分别为21.74%、35.00%和45.00%;添加牛粪提高了长期不同施肥潮土有机碳、微生物量碳、颗粒有机碳和易氧化有机碳含量,却显著降低了可溶性有机碳含量;牛粪对长期不施肥、长期施用常量有机肥、常量化肥和常量有机无机配施潮土有机碳矿化的正激发效应分别达到了48.56%、3.60%、48.43%和3.92%,且对长期不施肥及长期施用常量化肥潮土的激发效应显著高于对长期施用常量有机肥及长期有机无机配施土壤;冗余分析显示添加牛粪对长期不同施肥土壤有机碳矿化的激发效应与土壤活性组分碳氮比呈正相关,与土壤养分含量呈负相关。该研究不仅为合理施用有机肥和实现农田生态系统的可持续发展提供理论依据,还有利于实现农业资源再利用及其效益最大化。     

长期施肥对华北平原一种潮土有机氮组成的影响

In order to illustrate the change of nitrogen （N） supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the soil-climatic conditions of the North China Plain, organic N forms were quantified in the topsoil with different manure and chemical fertilizer treatments in a 15-year fertilizer experiment in a Chinese calcareous alluvial soil. Soil total N （TN） and various organic N forms were significantly influenced by long-term application of chemical fertilizer and manure. TN, total hydrolysable N, acid-lnsoluble N, amino acid N and ammonium N in the soil increased significantly （P 〈 0.05） with increasing manure and fertilizer N rates, but were not influenced by increasing P rates. Also, application of manure or N fertilizer or P fertilizer did not significantly influence either the quantity of amino sugar N or its proportion of TN. Application of manure significantly increased （P 〈 0.05） hydrolysable unknown N, but adding N or P did not. In addition, application of manure or N fertilizer or P fertilizer did not significantly influence the proportions of different soil organic N forms.     

长期定位施肥对潮土有机氮组分和有机碳的影响

利用河南封丘潮土的13年长期施肥试验,采用Bremner法研究了潮土耕层有机氮组成的变化,分析了长期施肥对土壤有机氮组份和有机碳含量的影响。与不施肥和单施化肥相比,施有机肥或有机肥与化肥配施显著提高了土壤全氮、酸解有机氮、酸解铵态氮、氨基酸态氮、非酸解有机氮和有机碳的含量。有机氮主要由酸解铵态氮和氨基酸态氮组成,其次为酸解未知态氮和非酸解有机氮,氨基糖态氮含量最小。施有机肥尤其利于氨基酸态氮和非酸解有机氮的形成。施肥处理的酸解有机氮占全氮的比例减小,主要是由氨基酸态氮、酸解铵态氮占全氮的比例减小所致。与1989年试验开始时的土壤初始值相比,施有机肥能提高土壤全氮含量和有机质含量。在供应等氮磷钾的情况下,有机无机配施增加了土壤供氮能力、有机质含量和作物产量,是维持土壤肥力和保护环境最优的施肥方式。     

过量施氮对旱地土壤碳、氮及供氮能力的影响

【目的】过量施氮会影响土壤有机碳、氮的组成与数量,进而改变土壤供氮能力,但关于西北旱地长期过量施用氮肥后土壤有机碳、氮及土壤供氮能力变化的研究尚缺乏。本文在长期定位试验的基础上,通过分析不同氮肥水平特别是过量施氮条件下土壤硝态氮,有机碳、氮和微生物量碳、氮的变化,探讨长期过量施氮对土壤有机碳、氮及供氮能力的影响。【方法】长期定位试验位于陕西杨凌西北农林科技大学农作一站。在施磷(P2O5)100kg/hm2的基础上,设5个氮水平,施氮量分别为N 0、80、160、240、320 kg/hm2。重复4次,小区面积40 m2,完全随机区组排列。种植冬小麦品种为小堰22。本文选取其中3处理,以不施氮为对照(N0)、施氮量N 160 kg/hm2为正常施氮(N160),施氮量N 320 kg/hm2为过量施氮(N320),分别于2012年6月小麦收获后和10月下季小麦播前采集土壤样品,进行测定分析。【结果】过量施氮导致下季小麦播前0—300 cm各土层硝态氮含量显著增加,平均由对照的2.8 mg/kg增加到15.5 mg/kg;同时,0—60 cm和0—300 cm土层的硝态氮累积量分别由对照的47.2和108.9 kg/hm2增加到76.5和727.7 kg/hm2。过量施氮也增加了夏闲期间0—300 cm土层土壤有机氮矿化量,由对照的72.4 kg/hm2增加到130.7 kg/hm2。但过量施氮未显著增加土壤的有机碳含量,却显著增加了土壤有机氮含量,过量施氮0—20、20—40 cm土层土壤有机碳分别为9.24和5.39 g/kg,有机氮分别为1.05和0.71 g/kg,较对照增加52.2%和54.3%。同样,过量施氮未显著影响0—20、20—40 cm土层土壤微生物量碳含量,其平均含量分别为253和205 mg/kg,却显著提高了0—20、20—40 cm土层土壤微生物量氮含量,由对照的24.1和7.5 mg/kg提高到43.6和16.1 mg/kg。【结论】过量施氮可以显著增加旱地土壤剖面中的硝态氮累积量、夏闲期氮素矿化量、小麦播前土壤氮素供应量和土壤微生物量氮含量,但对土壤有机碳和微生物量碳没有显著性影响,同时过量施氮增加了土壤硝态氮淋溶风险,故在有机质含量低的黄土高原南部旱地冬小麦种植中不宜施用高量氮肥,以减少土壤氮素残留和农业投入,达到保护环境和培肥土壤的目的。     

长期施肥棕壤团聚体分布及其碳氮含量变化

【目的】探究玉米-玉米-大豆轮作体系不同施肥处理对土壤团聚体分布及其有机碳、全氮的影响,以期深入了解施肥对土壤培肥、改善土壤结构的机制。【方法】选取不施肥（CK）,化肥（NPK）,低量有机肥（M1）,低量有机肥与化肥配施（M1NPK）,高量有机肥（M2）,高量有机肥与化肥配施（M2NPK）6个处理。采集棕壤37年长期定位试验微区不同施肥处理的0-20 cm和20-40 cm土样,分析其水稳性团聚体（ 1 mm、1~0.5 mm、0.5~0.25 mm、0.25~0.053 mm及 0.053 mm）分布及其有机碳、全氮分配特征。【结果】棕壤长期施肥对团聚体分布及其碳氮的影响0-20 cm大于20-40 cm,随土层深度的增加,有机碳（SOC）、全氮（TN）含量减少。各处理团聚体及碳、氮在团聚体中的分配主要在黏粉粒中（40%以上）。与CK相比,NPK处理显著提高了黏粉粒的含量,降低大团聚体与微团聚体含量,显著增加黏粉粒储碳比例;M1、M2处理显著增加 1 mm团聚体数量及其SOC含量,显著增加 0.25 mm各粒级团聚体的储碳比例,且M2处理显著高于M1处理;M1NPK、M2NPK处理也显著增加 1 mm团聚体数量及其SOC含量,M1NPK与M2NPK处理在NPK处理的基础上依次增加0.5~0.25 mm（M1NPK）、1~0.5 mm及 1 mm团聚体的储碳比例,M2NPK处理 0.25 mm团聚体储碳比例最高,土壤团聚体全氮的变化趋势与有机碳类似。【结论】棕壤连续有机无机配合施用可显著增加土壤大团聚体数量、SOC、TN含量及其储碳、氮比例,是提高土壤质量、改善土壤结构的有效施肥措施。     

Effects of different continuous fertilizer managements on soil total nitrogen stocks in China: A meta-analysis

Soil total nitrogen is critical for crop productivity and related to agricultural managements. However, the effects of different fertilizer applications on soil total nitrogen storage are not well understood. To quantify soil total nitrogen storage under different fertilizer management practices and explore the effects of climate, soil texture, experimental duration, and cropping system on soil total nitrogen storage in China, we conducted a meta-analysis of 67 fertilizer management strategies from experiments conducted over a period of at least three years. This meta-analysis included 854 observations of changes in soil total nitrogen stock(TNS) under no fertilizer application(control, CK), chemical fertilization with nitrogen, phosphorus, and potassium(CF), CF plus straw retention(CFS), and CF plus manure addition(CFM) relative to initial soil TNS. The CFM and CFS treatments increased soil TNS, and the CFM treatments increased soil C/N ratio the most. The longer the experimental duration, the greater the increase in soil TNS in the CF, CFS, and CFM treatments.Soil texture and crop type significantly affected the changes in soil TNS. The experimental duration, initial soil TNS, soil C/N ratio, and cropping system had significant linear correlations with the change in soil TNS. Temperature and precipitation were not correlated with soil TNS. Results of random forest modeling indicated that the most important factor affecting changes in soil TNS was experimental duration(positive correlation), followed by initial soil TNS(negative correlation). The CFM treatments had the largest increase in soil TNS under various conditions. We recommend promoting CFM to improve soil fertility in farmlands globally.     

有机无机配施比例对华北褐土冬小麦产量与氮肥利用率的影响

以保麦9号为试材,在氮磷钾用量一致的基础上,研究了有机无机氮配施比例对土壤有机质和养分含量、冬小麦产量和氮素利用率的影响。结果表明:施用无机氮和有机氮均能提高土壤有机质和养分含量,与对照相比,单施无机氮耕层土壤有机质增加1.7%,有机无机氮配施增加7.8%~11.2%,单施有机氮增加23.6%;土壤有效磷和速效钾变化趋势与有机质基本一致。施用无机氮和有机氮均可提高冬小麦的产量、蛋白质含量和氮肥利用率,以35%M和50%M处理最佳,小麦产量比对照增加2 292和2 419 kg/hm2,比单施无机氮增加656和783kg/hm2。相对于单施无机氮处理较高的小麦氮肥利用率(38.6%),35%M和50%M处理的氮素损失少,氮肥利用率高达47.9%和53.0%,高于单施化肥处理。说明在等氮量水平下,以35%和50%有机氮替代无机氮不但提高土壤有机质、养分含量、作物产量和氮肥利用率,还可减少氮素损失,可获得较高的经济和环境效益。     

长期施肥对土壤微生物生物量碳、氮及矿质态氮含量动态变化的影响

利用位于陕西杨凌的17年长期定位试验研究了长期不施肥(CK)、单施化肥(F)、化肥配施有机肥(F+M)和化肥加秸秆还田(F+S)处理对小麦-玉米轮作体系中作物不同生长时期土壤微生物生物量碳、氮(SMBC、SMBN)和矿质态氮含量的影响。结果表明,0—10 cm土层土壤SMBC、SMBN和矿质态氮含量的变化范围分别为264.8~752.2、37.51~14.8和3.83~8.5 mg/kg。不同处理相比,F+M处理中各采样时期(小麦苗期、拔节期、灌浆期及玉米播种期、大喇叭口期、灌浆期和收获后)土壤SMBC和SMBN含量均为最高,分别为不施肥对照的1.382~.65和1.892~.50倍;F+S处理矿质态氮含量最高,SMBC和SMBN也高于F和CK处理,大部分采样时期的差异达显著水平(P0.05);与CK相比,长期单施化肥也使各时期SMBC和SMBN含量提高。在小麦拔节期到灌浆期的旺盛生长阶段各施肥处理土壤SMBN含量均下降,而矿质态氮含量变化不大,处于较低水平;在玉米大喇叭口期到灌浆期的旺盛生长阶段,F+M、F+S和F处理土壤矿质态氮含量显著下降,而SMBN含量均有所升高。表明在土壤矿质态氮含量较高时,作物首先利用矿质态氮,而在土壤矿质态氮含量处于较低水平时,微生物固持的氮素可能会释放出来供作物吸收利用。     

Effect of water and nitrogen management on aggregate size and carbon enrichment of soil in rice‐wheat cropping system

A study was carried out on a silty clay loam soil (Typic Haplustept) to evaluate the effect of farmyard manure (FYM) vis‐à‐vis fertilizer and irrigation application on the soil organic C content and soil structure. The fertilizer treatments comprised of eight different combinations of N and FYM and three water regimes. The results indicated that the application of FYM and increasing N rate increased soil organic carbon (SOC) content. Addition of FYM also increased the percentage of large sized water stable aggregates (> 5 mm) and reduced the percentage of smaller size aggregates. This was reflected in an increase in the mean weight diameter (MWD) and improved soil structure. The organic carbon content in macroaggregates (> 1 mm) was greater compared to microaggregates, and it declined with decrease in size of microaggregates. This difference in organic C content between macro‐ and microaggregates was more with higher N dose and FYM treated plots. The effect of residual FYM on MWD and organic C content of the soil after wheat harvest was not significant. The effect was less in deeper layers compared to surface layers of the soil. MWD was significantly correlated with the SOC content for the top two layers.