Counter immunoelectroosmophoresis in the diagnosis of infectious bursal disease of poultry

Summary Infectious bursal disease virus antigen was detected in the bursa of Fabricius of infected birds by the use of the counter immunoelectroosmophoresis technique. Eight suspected outbreaks were confirmed in this way and 82 out of 89 bursal samples submitted for laboratory confirmation were positive. The test was also suitable for the detection of antibody to infectious bursal disease virus in sera of infected birds. Precipitin lines were visible within 30 min as compared with 18 to 24 h with the Ouchterlony agar gel precipitation test. Counter immunoelectroosmophoresis is recommended for rapid confirmation of infectious bursal disease of poultry.

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Resumen Mediante el empléo de la técnica de contra inmunoelectroosmoforesis, se detectó antigeno viral en la bolsa de Fabricio de aves infectadas con Gumboro. Se confirmaron ocho brotes sospechosos mediante la técnica, y 82 de 89 muestras tomadas de bolsas sospechosas y analizadas en el laboratorio fueron positivas. La prueba tambien fue útil para la detección de anticuerpos de Gumboro en el suero de animales infectados. Las lineas de precipitación fueron visibles a los 30 minutos en comparación a las 18 a 24 horas necesarias para leer la prueba de Ouchterlony. Se recomienda la contra inmunoelectroosmoforesis para el diagnóstico de la enfermedad de Gumboro en aves.

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Résumé L'antigène du virus de la bursite infectieuse aviaire a été détecté dans les bourses de Fabricius d'oiseaux infectés en utilisant la technique de contre immuno-électro-osmo-phorèse. Huit foyers suspects ont été confirmés dans cette manière et, sur 89 prèlevements de bourses soumis pour confirmation de diagnostic au laboratoire, 82 ont été trouvés positifs. Le test est indiqué pour la détection d'anticorps anti-virus de la bursite infectieuse aviaire dans les sérums d'oiseaux infectés. Des lignes de précipitation sont visibles dans les trente minutes alors qu'elles n'apparaissent qu'en 18–24 heures avec le test d'Ouchterlony en gélose. La contre immuno-électro-osmo-phorèse est recommandée pour une confirmation rapide de la bursite infectieuse aviaire.

     

A study of the epidemiology of infectious bursal disease in poultry in Queensland, 1976–1979

The epidemiology of infectious bursal disease (IBD) was studied by serology and sometimes by visual examination of the bursa of Fabricius in poultry flocks in Queensland during 1976–1979.
Ten flocks, each of approximately 30,000 meat breeding chickens, were surveyed. All chickens had maternally-derived antibody against IBD virus (IBDV) at hatching and active antibody was not detected while the chickens were brooded on rearing farms. When distributed to breeding farms, 7 of the flocks developed antibody when 11 to 25 weeks of age. The remaining 3 flocks were vaccinated by infection of 10% of the birds and within 4 weeks more than 80% of the chickens had developed precipitating antibody to IBDV.
Blood samples of 20 to 30 broiler chickens were collected at slaughter (7 to 9 weeks of age) from each of 312 broiler flocks raised on 37 contract farms. While the samples from 21 flocks were without detectable antibody to IBDV, all serum samples for 263 flocks contained antibody. The ratio of bursal weight to bodyweight was significantly lower in birds from 144 flocks having antibody to IBDV than in birds from 10 flocks that were without detectable antibody. In sequential studies, IBDV antibody became demonstrable in 27 of 30 flocks when the chickens were one to 6 weeks of age and was accompanied by bursal atrophy.
Serological investigation of 4 flocks of layer breeding chickens on a multi-age farm at approximately monthly intervals resulted in antibody to IBDV being detected at every examination.
Serological tests and bursal examinations were carried out weekly in 2 flocks each of 4000 layer chickens between one and 20 weeks of age. Serum antibody developed in one flock at 4 weeks of age and in the other at 17 weeks of age. In both flocks, bursal atrophy occurred concurrently with the development of antibody.     

鹌鹑法氏囊病的应对措施

近年来在生产中鹌鹑的法氏囊病时有发生。笔者于2008年3B初确诊了一起鹌鹑患传染性法氏囊病的病例,由于及时采取了防治措施病情得到有效控制,避免了更大的损失。     

鸡传染性法氏囊病的免疫接种对策

鸡传染性法氏囊(简称IBD)是一种高度接触性传染性且对生产危害极大的免疫抑制性疾病.3～6周龄的雏鸡最易感染,且发病迅速,病情较重.另外,由于法氏囊发炎,使其免疫功能受到破坏,因而雏鸡经接种MD、ND等疫苗后不能产生理想的免疫反应,甚至免疫失败.据统计,IBD发病率一般为80%～95%,高的可达100%,死亡率为20%～35%.有的高达50%,呈尖峰式死亡曲线.     

Effect of bursal cell number on the pathogenesis of infectious bursal disease in chickens

The pathogenesis of infectious bursal disease (IBD) in chickens neonatally chemically bursectomized (CB) by cyclophosphamide and subsequently inoculated with various numbers of bursal cells was examined. CB chickens inoculated with at least 62.5 X 10(6) bursal cells were as susceptible to IBD clinical manifestations (as determined by gross and microscopic evaluation of bursal tissues, virus recovery from spleen, and antibody titer) as intact chickens following inoculation with virus at 5 weeks of age. In contrast, CB chickens inoculated with 2.5 X 10(6) or fewer bursal cells were refractory to the IBD clinical manifestations compared with intact chickens or CB chickens inoculated with 62.5 X 10(6) or more bursal cells. Results from this study suggest that the availability of a large number of bursal cells is an essential factor in the development of IBD.     

谨防非典型传染性法氏囊病

传染性法氏囊病(IBD)是雏鸡和育成鸡的一种急性、接触性、免疫抑制性传染病。我国刘福致等于1979年在北京郊区一些鸡场发现该病并分离到病毒。1981～1989年全国患IBD的病鸡达2000万只,死亡240万只,死亡率11.8%。病鸡主要表现为精神不振、厌食、间歇性腹泻、震颤和高度虚脱,剖检以脱水、肌肉出血、肾小管尿酸盐沉积和法氏囊肿大、出血为特征,呈典型的IBD症状。近几年来,随着IBD弱毒疫苗的广泛使用,特别是使用了中等毒力的疫苗,基本上控制了典型IBD的发生,但是非典型IBD却常发不断,给养鸡业,特别是肉鸡饲养业带来严重的经济损失。1非…     

对某鸡场传染性法氏囊病的诊治体会

传染性法氏囊病是养鸡场常发的一种急性传染病，其发病率高、病程短、死亡率高，雏鸡感染后还可导致免疫抑制，并可诱发多种疫病或使多种疫苗免疫失败。最常见的是导致鸡新城疫、鸡传染性支气管炎的免疫失败，给养鸡业带来巨大的经济损失。我县某养鸡场今年年初暴发该病，现将其诊断及治疗过程报告如下。     

鸡传染性法氏囊病的诊治报告

自2005年春季以来,法库县频繁发生鸡传染性法氏囊病,其发病日龄逐渐拓宽,最早7日龄,最迟120日龄。临床表现及病理变化逐渐趋向非典型性,尖峰式零星死亡,现将诊治情况报告如下。     

Studies on a vaccine against infectious bursal disease

An infectious bursal disease vaccine, registered for use in breeder flocks, was studied for efficacy on the day-old offspring of vaccinated hens and for virulence in susceptible day-old and 6-week-old chickens. When given to susceptible day-old chicks and 6-week-old cockerels, the vaccine was found to induce atrophy and pathology of the bursa of Fabricius similar to that observed in field infections. Chicks vaccinated at day-old had markedly lowered titres in the haemagglutination inhibition test to Newcastle disease virus, when this was given 2 weeks later, but the response of the 6-week-old cockerel was similar to that of control birds. Maternal antibody induced by the vaccine protected chicks against infection at day-old.     

鸡传染性法氏囊病的诊断

鸡传染性法氏囊炎病是一种主要危害鸡的体液免疫中枢器官——法氏囊的病毒性传染病。牡丹江市某大型养鸡场2006年10月,购自某种鸡场海     

鸡传染性法氏囊病病毒的快速检测

随着 IBDV变异株的出现 ,发病鸡常不产生法氏囊的肉眼病变 ,由于传染性和非传染性因素亦可引起淋巴细胞减少和法氏囊坏死 ,所以 ,采取组织病理学方法诊断 IBDV感染并不完全可靠。由于主动性抗体应答需要一定的时间及大多数雏鸡都带有母源抗体 ,因此血清学早期诊断也不完全可靠。该试验采用本所研究的 IBD快速试纸与经典的琼扩试验 ,在对 IBDV的检测效果上进行了详细的比较 ,介绍如下。1　材料与方法1 .1　 IBD快速检测试纸　由河南省农业科学院生物技术研究所制备提供。1 .2　试验鸡　 1日龄试验鸡 30 0只由河南农业大学试验鸡场提…     

传染性法氏囊病病毒抗原表位分析--单克隆抗体的制备与鉴定

将传染性法氏囊病病毒(IBDV)分离毒株ZB和TA3的细胞培养物采用不连续蔗糖密度梯度离心的方法浓缩纯化病毒,免疫BALB/c小鼠,运用淋巴细胞杂交瘤技术将免疫鼠脾细胞与SP2/0骨髓瘤细胞融合,建立了6株分泌抗IBDV单克隆抗体(McAb)的杂交瘤细胞系1C1、1E6、2B2、2G8、3A2、3E2。间接ELISA测定,杂交瘤细胞培养上清液的抗体效价为102,诱生腹水的抗体效价为106～107。相加ELISA分析,这6株单克隆抗体对应不同的病毒抗原表位。中和试验结果表明,2G8对病毒有较强的中和能力,腹水的中和效价为105。用2B1和3E2配对建立的夹心ELISA可特异地检测IB DV。     

Infectious bursal disease serology in New Zealand poultry flocks

Extract

Madam:– Infectious bursal disease (IBD) is recognised as a serious economically significant disease of poultry. N.H. Christensen in his letter to your Journal (1) has summarised the ways in which IBD causes such economic loss.     

Antigenic diversity of infectious bursal disease viruses

Statistically significant antigenic differences were detected among serotype I infectious bursal disease viruses (IBDV) using the virus-neutralization test. Eight serotype I commercial vaccine strains, five serotype I field strains, and two serotype II field strains were tested. Hyperimmune guinea pig antisera against heterologous and homologous IBDV strains were used in cross-neutralization tests. Relatedness values were calculated from geometric mean antibody titers based on a minimum of three tests. Six subtypes were distinguished among the 13 serotype I strains tested.