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Sten E. Jensen 《Integrated Pest Management Reviews》2000,5(2):131-146
The western flower thrips, Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is a serious pest on a wide range of crops throughout the world. F. occidentalis is difficult to control with insecticides because of its thigmokinetic behaviour and resistance to insecticides. Pesticide resistance can have a negative impact on integrated pest management programmes with chemical control as one of the components. Resistance to a number of different insecticides has been shown in many populations of F. occidentalis. This flower thrips has the potential of fast development of resistance owing to the short generation time, high fecundity, and a haplodiploid breeding system. The mechanisms conferring insecticide resistance in insects can be divided into four levels. First, an altered behaviour can aid the insect to avoid coming into contact with the insecticide. Second, a delayed penetration through the integument will reduce the effect of the insecticide at the target site. Third, inside the insect, detoxification enzymes may metabolise and thereby inactivate the insecticide. Fourth, the last level of resistance mechanisms is alterations at the target site for the insecticide. Knowledge of resistance mechanisms can give information and tools to be used in management of the resistance problem. Recently, studies have been carried out to investigate the underlying mechanisms conferring resistance in F. occidentalis. It appears that resistance in F. occidentalis is polyfactorial; different mechanisms can confer resistance in different populations and different mechanisms may coexist in the same population. Possible resistance mechanisms in F. occidentalis include: reduced penetration, detoxification by P450-monooxygenases, esterases and glutathione S-transferases, and alterations of acetylcholinesterase, the target site for organophosphate and carbamate insecticides. Target site resistance to pyrethroids (knockdown resistance) may also be a resistance mechanism in F. occidentalis. 相似文献
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《Pesticide biochemistry and physiology》2009,95(2-3):49-54
Pyrethroid and organophosphate resistance-associated mutations have been recently reported in the whitefly Bemisia tabaci (Gennadius), a major pest of protected and outdoor crops worldwide. Here, we developed simple PCR–agarose gel visualization based assays for reliably monitoring the L925I and T929V pyrethroid resistance mutations in the B. tabaci para-type voltage gated sodium channel and the iAChE F331W organophosphate resistance mutation in the acetylcholinesterase enzyme ace1.PCR-RFLP assays were developed for detecting the L925I and the F331W resistance mutations. A highly specific PASA was developed for detecting the T929V mutation. The molecular diagnostic tools were used to monitor the frequency of the resistance mutations in a large number of field caught Q biotype B. tabaci from Crete (Greece), where both organophosphates and pyrethroids are extensively used. The F331W mutation was fixed in all field individuals examined. The pyrethroid resistance mutations were detected in high frequencies: 0.38 and 0.54 for L925I and T929V, respectively. The simple diagnostics are accurate and robust, to be used alongside classical bioassays to prevent ineffective insecticide applications, and for early identification of the spreading of resistant Q biotype populations into new regions around the globe. 相似文献
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Jérôme Carletto Thibaud Martin Flavie Vanlerberghe‐Masutti Thierry Brévault 《Pest management science》2010,66(3):301-307
BACKGROUND: The polyphagous cotton‐melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR‐RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1–38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7–6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super‐kdr (M918L) in the voltage‐gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races. Copyright © 2009 Society of Chemical Industry 相似文献
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灰飞虱对杀虫剂抗药性的研究进展 总被引:5,自引:0,他引:5
灰飞虱对杀虫剂产生抗药性是其近年来暴发频繁的重要原因。本文综述了国内外关于灰飞虱抗药性的研究成果,包括灰飞虱抗药性的发展、交互抗性、抗性机理、抗性遗传及生物适合度等。田间灰飞虱种群对多种药剂产生了不同程度的抗药性,其中对新烟碱类药剂吡虫啉和昆虫生长调节剂噻嗪酮产生了高水平到极高水平抗性(抗药性倍数分别为44.6~108.8倍和超过200倍),对有机磷类药剂毒死蜱和乙酰甲胺磷(抗药性倍数分别为10~12.6倍和9~13倍)、氨基甲酸酯类药剂甲萘威和残杀威(抗药性倍数分别为29.8~45.3倍和40.1~131.5倍)和拟除虫菊酯类药剂高效氯氰菊酯和溴氰菊酯(抗药性倍数分别为7.8~108.8倍和12~21倍)产生了中等水平到高水平的抗药性,对氟虫腈、阿维菌素和噻虫嗪没有产生抗药性(抗性倍数5倍)。长期大面积使用化学药剂是灰飞虱产生抗药性的重要原因。因此,必须加强灰飞虱的抗性治理,以延缓其抗药性进一步发展。 相似文献
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草地贪夜蛾Spodoptera frugiperda是一种迁飞性害虫,源自美洲,已入侵非洲和亚洲部分国家并暴发成灾,2019年初入侵我国,严重威胁着我国玉米、小麦等粮食作物。目前草地贪夜蛾的防治主要依赖于化学农药和种植转基因作物。因长期使用农药,草地贪夜蛾已经对氨基甲酸酯类、有机磷酸酯类和拟除虫菊酯类等多种杀虫剂产生了不同程度的抗性。草地贪夜蛾对不同种类杀虫剂表现的抗性机制不同,主要有代谢抗性和靶标抗性2种。本文主要从代谢抗性和靶标抗性2个方面分别综述了草地贪夜蛾对不同种类杀虫剂的抗性机制,分析草地贪夜蛾对不同杀虫剂的抗性作用方式,并对草地贪夜蛾抗药性管理和监测进行展望。 相似文献
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Jahangir Khajehali Thomas Van Leeuwen Maria Grispou Evangelia Morou Haoués Alout Mylène Weill Luc Tirry John Vontas Anastasia Tsagkarakou 《Pest management science》2010,66(2):220-228
BACKROUND: In Tetranychus urticae Koch, acetylcholinesterase insensitivity is often involved in organophosphate (OP) and carbamate (CARB) resistance. By combining toxicological, biochemical and molecular data from three reference laboratory and three OP selected strains (OP strains), the AChE1 mutations associated with resistance in T. urticae were characterised. RESULTS: The resistance ratios of the OP strains varied from 9 to 43 for pirimiphos‐methyl, from 78 to 586 for chlorpyrifos, from 8 to 333 for methomyl and from 137 to 4164 for dimethoate. The insecticide concentration needed to inhibit 50% of the AChE1 activity was, in the OP strains, at least 2.7, 55, 58 and 31 times higher for the OP pirimiphos‐methyl, chlorpyrifos oxon, paraoxon and omethoate respectively, and 87 times higher for the CARB carbaryl. By comparing the AChE1 sequence, four amino acid substitutions were detected in the OP strains: (1) F331W (Torpedo numbering) in all the three OP strains; (2) T280A found in the three OP strains but not in all clones; (3) G328A, found in two OP strains; (4) A201S found in only one OP strain. CONCLUSIONS: Four AChE1 mutations were found in resistant strains of T. urticae, and three of them, F331W, G328A and A201S, are possibly involved in resistance to OP and CARB insecticides. Among them, F331W is probably the most important and the most common in T. urticae. It can be easily detected by the diagnostic PCR‐RLFP assay developed in this study. Copyright © 2009 Society of Chemical Industry 相似文献
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Bioassays of Lacanobia subjuncta (Grote and Robinson) larvae established baseline LC50 values and identified the potential of reduced-risk, organophosphate replacement and naturally derived insecticides (eg chloronicotinyls, spinosyns, oxadiazines, insect growth regulators, microbial insecticides and particle films) to control this pest. The toxicities of these products were compared with those of organophosphate, carbamate, chlorinated cyclodiene and synthetic pyrethroid insecticides used in the management of lepidopteran pests in Washington apple orchards. Field trials were conducted comparing candidate insecticides to conventional alternatives. Several new insecticides (eg spinosad, methoxyfenozide, indoxacarb and an aluminosilicate particle film) proved to be effective for the management of L subjuncta. We summarize the goals and challenges of developing an integrated pest management program for new and resurgent pests as insecticide tools continue to change, and propose a hypothesis for the sudden increase in pest status of L subjuncta based on organophosphate tolerances. The role of novel insecticides with unique modes of action in resistance management and the encouragement of biological control are also discussed. 相似文献
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BACKGROUND: Developing scientifically valid, economically acceptable insecticide resistance management (IRM) programs is critical for sustainable insect management. The diamondback moth, Plutella xylostella (L.), has demonstrated an ability to develop resistance to many different classes of insecticides, including proteins produced by the bacterium Bacillus thuringiensis Berliner (Bt). Recently it has developed resistance to the novel compounds spinosad and indoxacarb. In greenhouse cage experiments, a laboratory‐selected population of P. xylostella resistant to spinosad, indoxacarb and Bt was used to compare population growth and resistance evolution if these three insecticides were rotated or used in a mosaic fashion. RESULTS: The average population density through nine generations was lowest in the treatment in which the insecticide was rotated every generation (R‐1) (x? = 20.7 ± 3.20) compared with the treatment in which the insecticide was rotated every third generation (R‐3) (x? = 41.4 ± 17.6) or where the insecticides were applied as a mosaic (M) (x? = 41.8 ± 6.53). After nine generations, the survival of resistant individuals increased for each insecticide (7.2–73.5%) compared with the population without selection (CK) (0.73–3.1%). Survival on spinosad was significantly lower (23.7%) in the single‐generation rotation than for the other two treatments, both of which exceeded 72%. The calculated survival on all three insecticides treated simultaneously, according to the survival on each insecticide, was 0.26, 0.81 and 1.6% for R‐1, R‐3 and M treatments respectively. CONCLUSION: Results of both population density and resistance development indicated that insecticide rotation every generation was better for IRM than if the insecticide was rotated every third generation or if the three insecticides were applied as a mosaic. Copyright © 2010 Society of Chemical Industry 相似文献
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Toshio Shono 《Pesticide biochemistry and physiology》2004,80(2):106-112
Indoxacarb (DPX-MP062) is a recently introduced oxadiazine insecticide with activity against a wide range of pests, including house flies. It is metabolically decarbomethoxylated to DCJW. Selection of field collected house flies with indoxacarb produced a New York indoxacarb-resistant (NYINDR) strain with >118-fold resistance after three generations. Resistance in NYINDR could be partially overcome with the P450 inhibitor piperonyl butoxide (PBO), but the synergists diethyl maleate and S,S,S-tributyl phosphorothioate did not alter expression of the resistance, suggesting P450 monooxygenases, but not esterases or glutathione S-transferases are involved in the indoxacarb resistance. Conversely, the NYINDR strain showed only 3.2-fold resistance to DCJW, and this resistance could be suppressed with PBO. Only limited levels of cross-resistance were detected to pyrethroid, organophosphate, carbamate or chlorinated hydrocarbon insecticides in NYINDR. Indoxacarb resistance in the NYINDR strain was inherited primarily as a completely recessive trait. Analysis of the phenotypes vs. mortality data revealed that the major factor for indoxacarb resistance is located on autosome 4 with a minor factor on autosome 3. It appears these genes have not previously been associated with insecticide resistance. 相似文献
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Background
Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure.Results
A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates.Conclusion
Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献12.
Insecticide sprays were applied to Myzus persicae (Sulzer) populations carrying various combinations of three insecticide resistance mechanisms (esterase-based metabolic resistance and two target site mechanisms, known as MACE and kdr), supported on host plants growing in field simulator cages. The study showed that MACE confers extreme resistance to pirimicarb and triazamate (carbamate insecticides) but not to deltamethrin + heptenophos (16 + 1) (Decisquick) or dimethoate (an organophosphorus insecticide). Resistance to dimethoate depends solely on levels of esterase-based resistance, while resistance to Decisquick depends on kdr and esterase. None of the four insecticides is effective against aphids carrying MACE combined with extreme esterase-based resistance. This knowledge, in association with current monitoring of the mechanisms, will play an important role in making decisions on insecticide use against M persicae in the UK. © 1999 Society of Chemical Industry 相似文献
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At the core of an integrated pest management program for Pennsylvanian apple orchards is an integrated mite management program that is based on a natural enemy, the coccinellid Stethorus punctum punctum (LeConte). The program relies upon the principles of ecological selectivity (e.g. chemical selection, timing, dose and method of application) for the organophosphate and carbamate insecticides. During the last 20 years the tufted apple bud moth (TABM), Platynota idaeusalis (Walker), a direct pest of apple, has developed resistance to these two chemical classes. In an effort to address this growing resistance problem, an intensive research program was initiated in 1986 on how to manage insecticide resistance in TABM while preserving the integrity of the integrated mite management program. One aspect of this research program is the investigation of biochemical and genetic approaches to resistance, including an analysis of detoxification mechanisms, effects of host plant allelochemistry on resistance and detoxification enzyme activities, reversion, gene flow and the isolation and characterization of a gene for glutathione transferase from TABM. Management approaches that have been developed and successfully implemented include pheromone mating disruption, parasitoids, ground-cover management including insecticides, Bacillus thuringiensis Berl. products and insect growth regulators. Resistance management options for TABM are presented. ©1997 SCI 相似文献
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Nyoni BN Gorman K Mzilahowa T Williamson MS Navajas M Field LM Bass C 《Pest management science》2011,67(8):891-897
BACKGROUND: The tomato red spider mite, Tetranychus evansi (Baker and Pritchard), is a serious pest of solanaceous crops in many African countries. In this study an investigation has been conducted to establish whether mutation of the para‐type sodium channel underlies pyrethroid resistance in T. evansi strains collected in Southern Malawi. RESULTS: Two T. evansi strains from Malawi showed tolerance to the organophosphate chlorpyrifos and resistance (20–40‐fold) to the pyrethroid bifenthrin, but were susceptible to two contemporary acaricides (abamectin and fenpyroximate) in insecticide bioassays. Cloning of a 3.1 kb fragment (domains IIS5 to IVS5) of the T. evansi para gene from pyrethroid‐resistant and pyrethroid‐susceptible strains revealed a single non‐synonymous mutation in the resistant strains that results in an amino acid substitution (M918T) within the domain II region of the channel. Although novel to mites, this mutation confers high levels of resistance to pyrethroids in several insect species where it has always been associated with another mutation (L1014F). This is the first report of the M918T mutation in the absence of L1014F in any arthropod species. Diagnostic tools were developed that allow sensitive detection of this mutation in individual mites. CONCLUSION: This is the first study of pyrethroid resistance in T. evansi and provides contemporary information for resistance management of this pest in Southern Malawi. Copyright © 2011 Society of Chemical Industry 相似文献
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P. C. Mercer 《国际虫害防治杂志》2013,59(2):190-192
Abstract Organochlorine, organophosphate (o.p.) and carbamate insecticides have been extensively used in Australia to combat Lucilia cuprina Wied. the main initiator of fly-strike of sheep occurring wherever sheep are run on the continent. The improved mules operation, mid-season crutching and insecticides are important in the management of Merino sheep. Insecticides are particularly valuable against body strike, especially in young sheep, in years with intermittent rain during the warmer months. Resistance to larvicides developed in the blowfly over a period of 10y from 1957. The time between the introduction of various insecticides and development of resistance is considered and a comparison made between the emergence of resistance problems to the newer synthetic insecticides in the housefly in Denmark, and in the blowfly in Australia. To provide a complete history of insecticides against the blowfly reference is made to arsenicals, to which there was no suspicion of resistance until low order cross resistance was diagnosed in o.p.-carbamate resistant strains. Some seven years after their introduction DDT and γ BHC were replaced by cyclodiene insecticides in 1954/1955. Reasons are advanced to explain the non-emergence of resistance to DDT and BHC in that period. Resistance to dieldrin and aldrin developed in late 1957 after which diazinon was introduced. The resistance has a typical BHC/dieldrin resistance spectrum and is due to a semi-dominant gene which has persisted in the field in the absence of pressure from cyclodiene insecticides. Non-specific resistance to o.p. insecticides developed in two steps. Low order resistance, diagnosed in 1965, was supplemented by an additional resistance mechanism in 1966, three alleles on two chromosomes are involved. The carbamate, butacarb, was effective against o.p. resistant strains of the blowfly when introduced in the 1966/67 season. In 1967, resistance to butacarb was diagnosed and rapidly became widespread. For the past three fly seasons larvae have been used to monitor resistance levels to o.p. and carbamate insecticides; resistance factors to both these classes of insecticide are significantly higher in larvae than adult females. Resistance levels to o.p. insecticides have stabilised. By contrast resistance levels to butacarb have doubled. A combined o p.-carbamate resistance generally applies in field samples. Larvae from o.p.-carbamate resistant strains form artificial strikes earlier than susceptible larvae, particularly on sheep treated with butacarb. It is concluded that registered o.p. insecticides, but not butacarb, will still give considerable protection against fly-strike, providing the maximum levels of o.p. resistance, reached in laboratory selection programmes, are not exceeded in the field. Investigation into o.p. resistance in the species suggests that the resistance mechanisms place their carriers at a disadvantage in the absence of selection pressure. Measures to minimise the amount of insecticide used against the blowfly are therefore strongly advocated—these include: the improved mules operation, mid season crutching and good animal husbandry. 相似文献
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Chung IH Kang S Kim YR Kim JH Jung JW Lee S Lee SH Hwang SY 《Pest management science》2011,67(12):1541-1548
BACKGROUND: Rapid and accurate detection of mutations related to insecticide resistance is essential for development of resistance management strategies to support sustainable agriculture. The M918V, L925I and T929V mutations of the voltage‐gated sodium channel gene (vgsc) and the F392W mutation of the acetylcholinesterase I gene (ace1) are reportedly associated with resistance to pyrethroids and organophosphates, respectively, in Bemisia tabaci. In order to detect known base substitutions in the ace1 and vgsc genes, a low‐density microarray with an allele‐specific probe was developed. RESULTS: Specific regions of the ace1 and vgsc gene mutations were amplified by multiplex asymmetrical PCR using Cy3‐labelled primers, and then the PCR products were hybridised on the microarray. After analysing the probe signal data, the microarray containing 12 allele‐specific probes produced a unique pattern of probe signals for field DNA samples of B. tabaci. To determine the optimal cut‐off value of each probe, receiver operating characteristic (ROC) curve analysis was conducted using SPSS. Among 60 individual samples, microarray data for 57 samples were consistent with direct sequencing data. CONCLUSION: Although many molecular detection methods have been employed to monitor insecticide resistance, the present microarray provides rapid and accurate identification of target mutations in B. tabaci for resistance management. Copyright © 2011 Society of Chemical Industry 相似文献
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Biochemical evidence that an S431F mutation in acetylcholinesterase-1 of Aphis gossypii mediates resistance to pirimicarb and omethoate 总被引:1,自引:0,他引:1
Molecular changes in acetylcholinesterase (AChE) leading to target-site resistance to carbamate and organophosphate insecticides have recently been identified. Of particular interest is the S431F mutation in ace2 and its orthologue ace1 of Myzus persicae Sulzer and Aphis gossypii Glover, respectively. This mutation has been correlated with resistance to pirimicarb, but biochemical evidence has not yet been provided. Here, we describe for the first time that recombinantly expressed AChE1 from A gossypii carrying the S431F mutation is insensitive to pirimicarb and omethoate, but sensitive to demeton-S-methyl and hypersensitive to carbofuran. Furthermore, site-directed mutagenesis of the serine residue at position 431 in ace1 from a pirimicarb-susceptible clone of A gossypii conferred insensitivity to pirimicarb. We conclude that AChE1 of A gossypii is the target of toxicological relevance of carbamates and organophosphates. 相似文献
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为了解我国不同地区棉蚜Aphis gossypii对吡虫啉和氟啶虫胺腈的抗性现状,对代表性棉区棉蚜田间种群进行抗药性监测,同时通过构建具有R81T及V62I单突变和R81T-V62I共同突变的棉蚜烟碱型乙酰胆碱受体(nicotinic acetylcholine receptor,nAChR)蛋白模型,与吡虫啉和氟啶虫胺腈进行分子对接,分析这些突变在吡虫啉和氟啶虫胺腈抗性中的作用,并分析吡虫啉和氟啶虫胺腈之间是否存在交互抗性。结果显示,不同地区棉蚜对吡虫啉产生了高水平抗性,抗性倍数为174.70~56 409.18,对氟啶虫胺腈产生了低至中等水平抗性,抗性倍数为7.35~44.63,说明不同地区的棉蚜对氟啶虫胺腈的敏感度高于吡虫啉,且吡虫啉抗性和氟啶虫胺腈抗性间不存在相关性。R81T、V62I单突变和R81T-V62I共同突变导致吡虫啉与棉蚜nAChR的亲和力降低,对氟啶虫胺腈与棉蚜n AChR的结合无明显影响。R81T及V62I单突变和R81T-V62I共同突变导致棉蚜对吡虫啉产生靶标抗性,但是对氟啶虫胺腈的抗性无明显影响,这些突变不会导致吡虫啉与氟啶虫胺腈产生靶标突变的交互抗性。 相似文献