毒死蜱有毒代谢物3,5,6-TCP在土壤中的吸附-解吸研究

应用OECD106批平衡方法,研究了毒死蜱的有毒代谢物3,5,6-TCP在6种典型土壤中的吸附-解吸行为。结果表明：Elovich方程、双常数方程和抛物线扩散方程能较好地拟合3,5,6-TCP在第四纪红土、黑土、黄壤和褐土中的吸附动力学过程,而对紫色土和潮沙土的拟合度较低（拟合相关系数小于0.85）;应用Freundlich方程和线性方程拟合第四纪红土、黑土、黄壤和褐土的经验常数nfads均小于1（非线性吸附）,而紫色土和潮沙土的nfads值则接近于1（线性吸附）;3,5,6-TCP在6种土壤中解吸的滞后系数H值均大于1,即解吸速率大于吸附速率。6种土壤对3,5,6-TCP的吸附常数Kfads从1.37-6.74μg1-n·fmLn·fg^-1,吸附系数Kd值从0.50-1.30mL·g^-1,其中第四纪红土和黑土对其吸持力较强（Kd〉1）,因而更应注意环境安全;其他4种土壤的Kd值则均小于1,淋溶风险较大。     

Nitrogen availability to grain sorghum from organic and inorganic sources on sandy and clayey soil surfaces in a greenhouse pot study

In a greenhouse pot study, we examined the availability of N to grain sorghum from organic and inorganic N sources. The treatments were¹⁵N-labeled clover residues, wheat residues, and fertilizer placed on a sandy clay loam and loamy sand soil surface for an 8-week period. Soil aggregates formed under each soil texture were measured after 8 weeks for each treatment. Significantly greater ¹⁵N was taken up and recovered by grain sorghum in sandy clay loam pots compared with loamy sand pots. Greater ¹⁵N recovery was consistently observed with the inorganic source than the organic sources regardless of soil texture or time. Microbial biomass C and N were significantly greater for sandy clay loam soil compared with the loamy sand. Microbial biomass ¹⁵N was also significantly greater in the sandy clay loam treatment compared to the loamy sand. The fertilizer treatment initially had the greatest pool of microbial biomass ¹⁵N but decreased with time. The crop residue treatments generally had less microbial biomass ¹⁵N with time. The crop residues and soil texture had a significant effect on the water-stable aggregates formed after 8 weeks of treatments. Significantly greater water-stable aggregates were formed in the sandy clay loam than the loamy sand. Approximately 20% greater water-stable aggregates were formed under the crop residue treatments compared to the fertilizer only treatment. Soil texture seemed to be one of the most important factors affecting the availability of N from organic or inorganic N sources in these soils.Contribution from the MissouriAgricultural Experiment Station, Journal Series No.12131     

Maize growth responses to deep tillage, straw mulching and farmyard manure in coarse textured soils of N.W. India

Abstract. The effects of deep tillage, straw mulching and farmyard manure on maize growth in loamy sand and sandy loam soils were studied in experiments lasting three years. Treatments included all combinations of conventional tillage (10 cm deep) and deep tillage (35–40 cm deep), two farmyard manure rates (0 and 15 t/ha) and two mulch rates (0 and 6 t/ha), replicated three times in a randomixed block design.
Deep tillage decreased soil strength and caused deeper and denser rooting. Mulching decreased maximum soil temperature and kept the surface layers wetter resulting in better root growth. Farmyard manure also improved root growth, and the crop then extracted soil water more efficiently. All three treatments increased grain yield in the loamy sand, but in the sandy loam only tillage and farmyard manure increased yields significantly. Deep tillage and straw mulch effects varied with soil type and amount of rainfall in the growing season. In the loamy sand the mean responses to deep tillage and mulching were largest in a dry year. A tillage-mulch interaction was significant in the loamy sand.     

Response of soil exchangeable and crop potassium concentrations to variable fertilizer and cropping regimes in long-term field experiments on different soil types

Annual potassium (K) balances have been calculated over a 40‐year period for five field experiments located on varying parent materials (from loamy sand to clay) in south and central Sweden. Each experiment consisted of a number of K fertilizer regimes and was divided into two crop rotations, mixed arable/livestock (I) and arable only (II). Annual calculations were based on data for K inputs through manure and fertilizer, and outputs in crop removal. Plots receiving no K fertilizer showed negative K balances which ranged from 30 to 65 kg ha^?1 year^?1 in rotation I, compared with 10–26 kg ha^?1 year^?1 for rotation II. On sandy loam and clay soils, the K yield of nil K plots (rotation I) increased significantly with time during the experimental period indicating increasing release of K from soil minerals, uptake from deeper soil horizons and/or depletion of exchangeable soil K (K_ex). Significant depletion of K_ex in the topsoil was only found in the loamy sand indicating a K supply from internal sources in the sandy loam and clay soils. On silty clay and clay soils, a grass/clover ley K concentration of ～2% (dry weight) was maintained during the 40‐year study period on the nil K plots, but on the sandy loam, loam and loamy sand, herbage concentrations were generally less than 2% K.     

Simulating nitrate retention in soils and the effect of catch crop use and rooting pattern under the climatic conditions of Northern Europe

This model analysis of catch crop effects on nitrate retention covered three soil texture classes (sand, loamy sand, sandy loam) and three precipitation regimes in a temperate climate representative of northern Europe (annual precipitation 709–1026 mm) for a period of 43 years. Simulations were made with two catch crops (ryegrass and Brassica) with different rooting depths, and soil N effects in the next spring were analysed to 0.25, 0.75 and 2.0 m depth to represent the catch crop effect on following crops with different rooting depths. Nitrate retained without a catch crop was generally located in deeper soil layers. In the low precipitation regime the overall fraction of nitrate retained in the 0–2.0 m soil profile was 0.23 for the sandy soil, 0.69 for the loamy sand and 0.81 for the sandy loam. Ryegrass reduced leaching losses much less efficiently than Brassica, which depleted nitrate in the 0–0.75 m soil layer more completely, but also in the deeper soil layer, which the ryegrass could not reach. A positive N effect (N_eff, spring mineral N availability after catch crop compared with bare soil) was found in the 0–0.25 m layer (that is shallow rooting depth of a subsequent main crop) in all three soil texture classes, with on average 10 kg N/ha for ryegrass and 34 kg N/ha for Brassica. Considering the whole soil profile (0–2.0 m deep rooting of next crop), a positive N_eff was found in the sand whereas generally a negative N_eff was found in the loamy sand and especially the sandy loam. The simulations showed that for shallow‐rooted crops, catch crop N_eff values were always positive, whereas N_eff for deeper‐rooted crops depended strongly on soil type and annual variations in precipitations. These results are crucial both for farmers crop rotation planning and for design of appropriate catch crop strategies with the aim of protecting the aquatic environment.     

A study of some tillage practices for sustainable crop production in India

Soil tilth has been defined in terms of a ‘Physical Index’ based on the product of the ratings of eight physical properties — soil depth, bulk density, available water storage capacity, cumulative infiltration or apparent hydraulic conductivity, aggregation or organic matter, non-capillary pore space, water table depth and slope. The Physical Index and a tillage guide were used to identify the tillage requirements of different soils varying in texture from loamy sand to clay in the semi-arid tropics. The physical index was 0.389 for a loamy sand, 0.518 for a black clay loam and 0.540 for a red sandy loam soil and the cumulative rating indices in summer and winter seasons were 45 and 44 for loamy sand, 52 and 51 for red sandy loam and 54 and 52 for black clay loam soils, respectively. The compaction of the loamy sand by eight passes of a 490 kg tractor-driven roller (0.75 m diameter and 1.00 m length) increased the physical index to 0.658 and chiselling of the red sandy loam and black clay loam increased the physical indices to 0.686 and 0.729, respectively. The grain yields of rainfed pearl millet and guar and irrigated pearl millet, wheat and barley increased significantly over the control (no compaction) yields by compaction.

The chiselling of the soils varying in texture from loamy sand to clay at 50 to 120-cm intervals up to 30–40 cm depth, depending upon the row spacing of seedlines and depth of the high mechanical impedance layer, increased the grain yields of rainfed and irrigated maize on alluvial loamy sand, rainfed maize on alluvial sandy loam and red sandy loam, rainfed sorghum on red sandy loam and black clay loam, irrigated sorghum on black clay loam and rainfed black gram on red sandy loam, pod yield of rainfed groundnut, tuber yield of irrigated tapioca and fresh fruit yield of rainfed tomato on red sandy loam and sugarcane yield on black clay soil, significantly over the yields of no-chiselling systems of tillage such as disc harrow and country plough.     

Manipulating the N release from N-rich crop residues by using organic wastes on soils with different textures

The potential to manipulate the N release from vegetable crop residues (cauliflower, leek) by using organic wastes was tested under field conditions on three soil textures during 2 years (silt loam, sandy loam and loamy sand). During the first year, incorporation of green waste compost and sawdust did not significantly increase microbial biomass N and did not lead to a significant N immobilization of crop residue‐N. During the second year, straw did increase microbial biomass N and showed a good N immobilization potential in all textures. The largest increase in microbial biomass N and the greatest N immobilization occurred in the loamy sand soil. The texture effect was probably because of better incorporation of the crop residues and immobilizer wastes in the loamy sand soil compared with the other textures. During spring, there was no consistent remineralization of immobilized N after the addition of malting sludge or vinasses in either year. This could be a result of the limited amount of N immobilized and available for remineralization in the first year or an unsuitable composition of the remineralizer wastes.     

Crop yield and SOC responses to biochar application were dependent on soil texture and crop type in southern Quebec,Canada

Changes to soil nutrient availability and increases for crop yield and soil organic C (SOC) concentration on biochar‐amended soil under temperate climate conditions have only been reported in a few publications. The objective of this work was to determine if biochar application rates up to 20 Mg ha^?1 affect nutrient availability in soil, SOC stocks and yield of corn (Zea mays L.), soybean (Glycine max L.), and switchgrass (Panicum virgatum L.) on two coarse‐textured soils (loamy sand, sandy clay loam) in S Quebec, Canada. Data were collected from field experiments for a 3‐y period following application of pine wood biochar at rates of 0, 10, and 20 Mg ha^?1. For corn plots, at harvest 3 y after biochar application, 20 Mg biochar ha^?1 resulted in 41.2% lower soil NH on the loamy sand; the same effect was not present on the sandy clay loam soil. On the loamy sand, 20 Mg biochar ha^?1 increased corn yields by 14.2% compared to the control 3 y after application; the same effect was not present on the sandy clay loam soil. Biochar did not alter yield or nutrient availability in soil on soybean or switchgrass plots on either soil type. After 3 y, SOC concentration was 83 and 258% greater after 10 and 20 Mg ha^?1 biochar applications, respectively, than the control in sandy clay loam soil under switchgrass production. The same effect was not present on the sandy clay loam soil. A 67% higher SOC concentration was noted with biochar application at 20 Mg ha^?1 to sandy clay loam soil under corn.     

Comparison of sulphur uptake by oilseed rape and the soil sulphur status of two adjacent fields with different soil series

Abstract. At Woburn Experimental Farm (SE central England) sulphur (S) deficiency symptoms occurred in a winter oilseed rape crop grown in Lansome field on soil of the Cottenham series, but not on the Blithe series soil in the adjacent Mill Dam Close field. At maturity, the two crops produced a similar amount of dry matter but seed yield and harvest index were significantly less in Lansome. Total S uptake of the crop in Lansome was less than half of that in Mill Dam Close, which was similar to the amount normally found in a S sufficient crop. Both soils are of light texture (loamy sand and sandy clay loam for the Cottenham and Blithe series, respectively), and there was little difference in the extractable S concentration in the topsoils between the two fields. However, more extractable S was found in the subsoil of Blithe series, because it contains more free Fe and Al oxides and has a lower pH. These results show that any evaluation of the soil S supply needs to take into account the pool of available S in the subsoil.     

Straw incorporation and soil organic matter in macro-aggregates and particle size separates

Two field experiments in which straw has been removed or incorporated for 17 yr (loamy sand) and 10 yr (sandy clay loam) were sampled to examine the effect of straw on the C and N contents in whole soil samples, macro-aggregate fractions and primary particle-size separates. The particle size composition of the aggregate fractions was determined. Aggregates were isolated by dry sieving. Straw incorporation increased the number of 1–20 mm aggregates in the loamy sand but no effect was noted in the sandy clay loam. Straw had no effect on the particle size composition of the various aggregate fractions. After correction for loose sand that accumulated in the aggregate fractions during dry sieving, macro-aggregates appeared to be enriched in clay and silt compared with whole soil samples. Because of the possible detachment of sand particles from the exterior surface of aggregates during sieving operations, it was inferred that the particle size composition of macro-aggregates is similar to that of the bulk soil. The organic matter contents of the aggregate fractions were closely correlated with their clay + silt contents. Differences in the organic matter content of clay isolated from whole soil samples and aggregate fractions were generally small. This was also true for the silt-size separates. In both soils, straw incorporation increased the organic matter content of nearly all clay and silt separates; for silt this was generally twice that observed for clay. The amounts of soil C, derived from straw, left in the loamy sand and sandy clay loam at the time of sampling were 4.4 and 4.5 t ha^?1, corresponding to 12 and 21% of the straw C added. The C/N ratios of the straw-derived soil organic matter were 11 and 12 for the loamy sand and sandy clay loam, respectively.     

Cadmium soil sorption at low concentrations: II. Reversibility,effect of changes in solute composition,and effect of soil aging

Water, Air, & Soil Pollution - Desorption of Cd from two Danish soils (loamy sand, sandy loam) previously exposed to low concentrations of Cd was examined in terms of reversibility, effect of...     

Studies of 65Zn movement in soil columns under laboratory conditions

Zinc movement was studied in relation to moisture regime, element source, and method of application in soil columns constructed from samples of surface layers of Begu loamy sand and Gurgaon sandy loam. Maximum movement was only 10 mm laterally and 40 mm downward. The amount of zinc and the distance moved were greatest under the wettest regime. Movement was also greater in loamy sand than sandy loam columns, with Zn-EDTA rather than sulphate as the source, and when the amount of zinc was greater for a given volume of soil material.The effect of urea, monocalcium phosphate, calcium carbonate, and farmyard manure on the movement of zinc was determined at one moisture level in columns constructed from samples of surface layers of Begu loamy sand and Thana loam. The movement of zinc within the soil columns was reduced by all four treatments.     

Nitrate leaching from organic arable crop rotations: effects of location, manure and catch crop

Abstract. Nitrate leaching from crop rotations supporting organic grain production was investigated from 1997 to 2000 in a field experiment at three locations in Denmark on different soil types. Three experimental factors were included in the experiment in a factorial design: (1) proportion of N₂-fixing crops in the rotation (crop rotation), (2) catch crop (with and without), and (3) manure (with and without). Three, four-course rotations were compared, two at each location. The nitrate leaching was measured using ceramic suction cells. Leaching losses from the crop rotation with grass–clover green manure and without catch crops were 104, 54 and 35 kg N ha⁻¹ yr⁻¹ on the coarse sand, the loamy sand, and the sandy loam, respectively. There was no effect of manure application or time of ploughing-in the grass–clover green manure crop on the accumulated nitrate leaching from the entire rotation. Catch crops reduced nitrate leaching significantly, by 30–38%, on the sandy soils. At all locations catch crops reduced the annual averaged nitrate concentration to meet drinking water quality standards in the crop rotation with green manure. On the coarse sand there was a time lag between the onset of drainage and the start of N-uptake by the catch crop.     

The relationship between N mineralization or microbial biomass N with micromorphological properties in beech forest soils with different texture and pH

To test relationships between net N-mineralization, organic matter and soil organisms, we combined micromorphology with laboratory incubation experiments over a soil gradient. Microbial biomass N generally increased with pH, and from sandy to loamy soil, but net N-mineralization showed the opposite, and was highest in acid, sandy soil. Twenty-two micromorphological characteristics were analyzed with principal component analysis. PC1 had high eigenvalue (0.70), and clearly separated fungi from earthworms, microarthropods and bacteria. PC2 was less important (0.15). Organic layer and sand content clearly correlated with the fungi-end of PC1, but pH and C-content of the Ah with the opposite. Microbial N also correlated with the earthworm–bacteria end, but net N-mineralization did not. Efficiency of N-mineralization per unit microbe even correlated with the fungi end of PC1, in both organic layer and mineral topsoil. The results support the hypothesis that high (or low) litter turnover and biological activity can be counteracted by high (or low) microbial N-demand.     

The persistence and degradation of chlorothalonil and chlorpyrifos in a cranberry bog

The effect of a spray-tank adjuvant on the persistence, distribution, and degradation of two pesticides, chlorothalonil and chlorpyrifos, was studied in a commercial cranberry bog. Pesticides were applied according to label instructions to cranberry plants in paired plot studies. Dislodgeable foliar and whole fruit residues of both pesticides and several degradation products were assessed over a growing season. Residues were also assessed in soil samples collected at fruit harvest. Adjuvant increased both fruit and foliar residues but did not significantly alter the dissipation rate or metabolism of either pesticide. The dissipation of dislodgeable foliar chlorothalonil and chlorpyrifos residues followed first-order kinetics, with estimated half-lives of 12.7 and 3.5 d, respectively. All residue levels on harvested fruit were well below the current U.S. EPA tolerances for fresh cranberries. Chlorothalonil (58%) was the major residue in fruit at harvest (76 d post-chlorothalonil application), with 4-hydroxy-2,5,6-trichloroisophthalonitrile and 1,3-dicarbamoyl-2,4,5,6-tetrachlorobenzene accounting for 26% and 6% of the total residues, respectively. Degradation products accounted for 88% of the total chlorothalonil residues in soil at fruit harvest. The products 1,3-dicarbamoyl-2,4,5,6-tetrachlorobenzene, 1-carbamoyl-3-cyano-4-hydroxy-2,5,6-trichlorobenzene, 2,5,6-trichloro-4-methylthioisophthalonitrile, and 2,4,5-trichloroisophthalonitrile have not been previously identified in cranberry bog environments. Chlorpyrifos was detected in fruit at harvest (62 d post-chlorpyrifos application), but no metabolites were found. Chlorpyrifos-oxon and 3,5,6-trichloro-2-pyridinol, however, were detected in earlier fruit samples and in foliage and soil samples.     

Evidence of peptides in low-molecular-weight fractions of soil organic matter

Summary Organic matter was extracted from three soils, a Berwick sandy loam, a Franklin loamy sand, and a Cumberland silty loam. The extracts were separated into high (>8000 daltons) and low-molecular-weight (<8000 daltons) fractions using gel filtration. Reverse-phase high performance liquid chromatography at 214 nm was used to separate the peptides into low-molecular-weight fractions. Peptide samples were collected with an integrated fraction collector and hydolyzed with an immobilized protease column reactor. High performance liquid chromatography with fluorescence detection was used to determine the amino-acid contents of the collected samples. The results indicated that peptide intermediates are present in soil size fractions. Greater quantities of several amino acids were released from the peptide hydrolyzates of the Berwick sandy loam and Franklin loamy sand, compared with the Cumberland silty loam, an uncultivated soil. These findings indicate that organic intermediates (e.g., peptides) are more prevalent in biologically active soils than in relatively inert soils.     

DMPP硝化抑制效果受其浓度、施用方式及土壤基质势的影响

The efficacy of nitrification inhibitors depends on soil properties and environmental conditions. The nitrification inhibitor 3.4-dimethylpyrazole phosphate （DMPP） was investigated in a sandy loam and a loamy soil to study its effectiveness as influenced by inhibitor concentration, application form, and soil matric potential. DMPP was applied with concentrations up to 34.6 mg DMPP kg^-1 soil as solution or as ammonium-sulfate/ammonium-nitrate granules formulated with DMPP. DMPP inhibited the oxidation of ammonium in both soils, but this effect was more pronounced in the sandy loam than in the loamy soil. When applied as solution, increasing DMPP concentrations up to 7 mg DMPP kg^-1 soil had no influence on the inhibition. The effectiveness of DMPP formulated as fertilizer granules was superior to the liquid application of DMPP and NH4^＋, particularly in the loamy soil. Without DMPP, a decline in soil matric potential down to -600 kPa decreased nitrification in both soils, but this effect was more pronounced in the sandy loam than in the loamy soil. DMPP was most effective in the sandy loam particularly under conditions of higher soil moisture, i.e., under conditions favorable for nitrate leaching.     

Decomposability of organic matter in particle size fractions from field soils with straw incorporation

Soils from two field experiments on straw disposal were fractionated according to particle size using ultrasonic dispersion and gravity-sedimentation in water. Samples of whole soils, clay. silt and sand-size fractions were held for 49 days at 20°C and the CO₂ evolution measured on 14 dates by gas chromatography.Recovery of soil solids. C and N was 99, 98 and 93%, respectively. Most of the soil C and N was in the clay (<2μm). (loamy sand, 50% C and 56% N; sandy loam. 65% C and 68% N), the silt (2–20 μm) having smaller proportions (loamy sand, 41% C and 38% N; sandy loam. 29% C and 27% N). The sand fraction (20–6000 μm) accounted for 4–7% of the organic matter, and 1–2% of the C was water soluble. Straw incorporation generally increased the C and N content of whole soils and size fractions.The decomposition rate constants were higher for the sandy loam than for the loamy sand soil. For both soils, the decomposability of the organic matter decreased in the order: sand > clay ⩾ whole soil > silt. Straw incorporation increased the decomposition rate of whole soil and sand organic matter. whereas the effect of straw on clay and silt respiration was small.Between 58 and 73% of the respiration was from clay, 21–25% from silt and 6–19% from the sand size fraction.     

Spatial variability of Southeastern U.S. Coastal Plain soil physical properties: Implications for site-specific management

Our objectives were to describe the field-scale horizontal and vertical spatial variability of soil physical properties and their relations to soil map units in typical southeastern USA coastal plain soils, and to identify the soil properties, or clusters of properties, that defined most of the variability within the field. The study was conducted on a 12-ha field in Kinston, NC. A 1:2400 scale soil survey had delineated three soil map units in the field: Norfolk loamy sand, Goldsboro loamy sand, and Lynchburg sandy loam. These are representative of millions of hectares of farmland in the Coastal Plain of the southeastern USA. Sixty soil cores were taken to ∼ 1-m depth, sectioned into five depth increments, and analyzed for: soil texture as percentage sand, silt, and clay; soil water content (SWC) at − 33 and − 1500 kPa; plant available water (PAW); saturated hydraulic conductivity (Ksat); bulk density (BD); and total porosity. A penetrometer was used to measure cone index (CI) at each sample location. Variography, two mixed-model analyses, and principal components analysis were conducted. Results indicated that soil physical properties could be divided into two categories. The first category described the majority of the within-field variability and included particle size distribution (soil texture), SWC, PAW, and CI. These characteristics showed horizontal spatial structure that was captured by soil map units and especially by the division between sandy loams and finer loam soils. The second class of variables included BD, total porosity, and Ksat. These properties were not spatially correlated in the field and were unrelated to soil map unit. These findings support the hypothesis that coastal plain soil map units that delineate boundaries between sandy loams versus finer loam soils may be useful for developing management zones for site-specific crop management.     

Performance assessment and validation of a paramagnetic particle-based enzyme-linked immunosorbent assay for chlorpyrifos in agricultural runoff waters

A commercial magnetic particle-based enzyme-linked immunosorbent assay (ELISA) kit for the insecticide chlorpyrifos [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate] was evaluated for its specificity, precision, and accuracy, its susceptibility to matrix interferences in agricultural and environmental surface waters, and its comparability to a gas chromatographic/flame photometric (GC/FPD) method for the determination of organophosphorus pesticides in natural waters. Repeatability, reproducibility, and accuracy studies show that the kit satisfies current U.S. Environmental Protection Agency criteria for the assessment of analytical methods. Observable matrix effects were found to be present in all of the environmental test waters, with the slopes of calibration curves generated in each of the test matrices deviating from that of the control matrix by as much as 16%. Specificity studies indicate that the chlorpyrifos polyclonal antibody adequately differentiates the target compound from other structurally similar organophosphorus pesticides, with the exception of its methyl analogue. Cross-reactivity with chlorpyrifos-methyl was approximately 37%, while reactivity with diazinon, pyridaphenthion, diclofenthion, bromiphos-ethyl, bromiphos-methyl, pirimiphos-ethyl, and chlorpyrifos oxon ranged from 1.6 to 10.7%. Cross-reactivity with pirimiphos-methyl, 3,5,6-trichloro-2-pyridinol, diethyl phosphate, and diethyl thiophosphate was negligible (<1%). Validation of the paramagnetic particle ELISA format was accomplished using water samples from two monitoring studies that were collected, split, and analyzed directly by ELISA and by GC/FPD. Results of the two analytical methods were then compared using standard t tests, regression analysis, and differences against mean measurement (bias) plots. While the agreement between the two methods was determined to be satisfactory, ELISA exhibits consistent positive bias in environmental matrices. Several preanalysis mitigation steps were suggested that may help moderate bias, but additional study is recommended to explicate the exact factors responsible for its consistent overestimation of results.