Changes in ascorbic acid content during growth and short-term storage of potato tubers (Solanum tuberosum L.)

Changes in ascorbic acid levels were followed in apical and basal portions of Russet Burbank and Kennebec tubers during growth and development. Influence of nitrogen fertilization during growth and short-term storage on ascorbic acid level of the tuber was also studied. Changes in ascorbic acid content during growth showed two distinct phases, the first phase being characterized by an increase in ascorbic acid content with growth and development of the tuber, followed by a second phase which showed a decrease in ascorbic acid content with increasing maturity. The shift from the first phase to the second phase occurred earlier for Kennebec than for Russet Burbank. Increased nitrogen fertilization resulted in a delay of this shift from the first to the second phase. The apical portion of the tuber consistently showed higher ascorbic acid content than the basal portion. Kennebec tubers were higher in vitamin C content than tubers of Russet Burbank cultivar. High nitrogen fertilization resulted in less ascorbic acid content during growth. Storage of tubers for four weeks at 5.5°C also resulted in a marked decrease in ascorbic acid content of tubers. Nitrogen fertilizer rate during growth had no influence on the decrease in ascorbic acid content of tubers in storage.     

Ultrastructural changes in tubers of potato (Solanum tuberosum L.) affected by rust spot

Summary The medullary tissues of potato tubers affected by rust spot were embedded in resin, sectioned and stained for optical and electron microscopy. Under the optical microscope, the damaged zones were seen to have thickened and suberized cell walls, with cells partially or totally lacking starch. These features were confirmed by electron microscopy which revealed in relief the cell wall deformation and the characteristic presence, in the vacuole, of osmiophilic granules with a spongy appearance attached to the tonoplast, probable catabolic products of a disturbed metabolism. Separation and degeneration of the plasmalemma and the death of the cells were observed in tissues in the final stages of damage.     

The nitrogen response of potato (Solanum tuberosum L.) in the field: nitrogen uptake and yield, harvest index and nitrogen concentration

Summary The response of potato to different rates of nitrogen supply ranging from 0–40 g m⁻² N was studied in five field experiments near Wageningen. NL (52⁰ North). In total two late potato cultivars and two sites were used during successive seasons. The results are summarized in a set of regression equations separately for total crop and tubers. The relation between nitrogen taken up (g m⁻²) in the total crop and total dry matter production (g m⁻²) could be described with the exponential equation: 1942–1900 * 0.93^X (r²=0.953, n=62). Nitrogen concentrations in the dry matter increased linearly with nitrogen uptake. Harvest indices for dry matter and nitrogen tended to decline with increase in N uptake. Cultivars differed only in the effect on N on tuber dry matter concentration. The relation between nitrogen uptake and nitrogen supply could be fitted with quadratic regression models. but coefficients were influenced by site and season.     

A Kunitz-type proteinase inhibitor with differential solubility during the development of potato (Solanum tuberosum L.) tubers

Summary Two potato (Solanum tuberosum L., cv. Irish Cobbler) Kunitz-type proteinase inhibitors (PKPI) were previously described to be present in the soluble fraction of proteins from tubers in the early stages of development. One of them became insoluble in mature tubers, being extractable from this material in presence of urea. Amino acid sequencing showed that the soluble and insoluble PKPI were identical to each other. Also, immunolocalization using the protein A-gold method showed that both proteins were present inside the vacuole in free (intravacuolar space) and aggregated forms. The density of PKPI in the vacuolar protein aggregates increased from developing to mature tubers. showing that the soluble-insoluble state of this protein is related to the aggregation levels. Purified PKPI precipitated in vitro. mainly in presence of high calcium concentrations and low pH, but this precipitated form was not as stable as aggregates found in vivo. Based on the results obtained, a model of PKPI insolubilization in vivo is discussed.     

Susceptibility of potato (Solanum tuberosum L.) foliage and tubers to the US8 genotype ofPhytophthora infestans

Late blight is an important disease of potato(Solanum tuberosum L.) worldwide, and therefore, many potato-breeding programs have prioritized the development of late blight-resistant potato cultivars. Although the emphasis has been to enhance foliar resistance, it is also necessary to evaluate tuber late blight resistance in new breeding lines and new sources of late blight resistance. We report here on the assessment of foliar and tuber resistance and the correlation between these aspects of resistance in a sample of Michigan State University potato breeding lines. Two MSU breeding lines had significantly less infected foliage than the susceptible check cultivars. Tuber susceptibility was significantly different (P < 0.05) only between the most susceptible and the least susceptible breeding lines/cultivars. Foliar and tuber susceptibility to potato late blight were not correlated as low tuber susceptibility was associated both with extremely low (e.g., MSG274-3) and high (e.g., MSE202-3Rus) foliar susceptibility.     

Effect of sprout inhibitors on potato tubers (Solanum tuberosum L.) stored at ambient or reduced temperatures

Summary Single and combined applications of three sprout inhibitors, maleic hydrazide (MH), isopropyl 3-chlorophenylcarbamate (CIPC) and isopropyl phenylcarbamate (IPC) mixed with CIPC in a commercial formulation coded AMK (Tixit-C, Sandoz, S.A.E., Barcelona, Spain; 2.0% IPC +0.7% CIPC) were tested on potatoes stored from March to June under ambient conditions (20–30°C, 30–80% RH), and in a store with passive evaporative cooling (16–30°C, 75–95% RH). All treatments were more effective in reducing physiological losses, sprouting, and sprout growth in the cool than in the ambient store. AMK, CIPC and MH+AMK caused most sprout suppression. All treatments increased tuber rotting but there were no significant differences in numbers rotted by MH, MH+CIPC, CIPC (single treatment) and the untreated control. Nevertheless, the treatments can usefully extend storage life of potatoes under non-refrigerated conditions.     

The effect of storage temperature on reducing sugar concentration and the activities of three amylolytic enzymes in tubers of the cultivated potato,Solanum tuberosum L.

Summary Reducing sugar content, and activities of three starch hydrolysing enzymes, alpha-amylase, beta-amylase and debranching enzyme were measured over several months in tubers of five cultivars stored at 4°C or 10°C. Cultivars differed in their sensitivity to storage temperature. Reducing sugar content of tubers and the activities of three starch hydrolysing enzymes increased sharply during the first weeks of storage at 4°C. At 10°C, reducing sugar content, and the activity of the three enzymes remained constant or increased only slightly. Deceased.     

High temperature storage of potato (Solanum tuberosum L.) for processing — a feasibility study

Tubers of three potato cultivars were stored at room temperature (20–39°C, 30–75% RH), under an insulated storage run on passive evaporative cooling (16–30°C, 70–90% RH) and a refrigerated storage (2–4°C, 90–95% RH) for 14 weeks and studied periodically for storage losses, reducing sugar content and dry matter percent of tubers, in order to explore the possibility of storing potatoes for processing at higher temperatures. Physiological losses in tubers remained less than 10% until 12 weeks of storage under evaporatively cooled storage. Reducing sugar contents increased by only 52.4–242.1% in tubers stored in evaporatively cooled storage as compared to 90.5–484.2% increase in tubers stored in refrigerated storage until 14 weeks. Potatoes stored in evaporatively cooled store were more suitable for processing into chips and french fries due to lower physiological losses and lower reducing sugar content of tubers.     

Possible mechanisms of size hierarchy among tubers on one stem of a potato (Solanum tuberosum L.) plant

Summary The tubers on one potato stem vary greatly in size and their size distribution may be described by their number and their average size and its variation. These components are mutually and closely related. The variation is partly caused by stolon characteristics, including their date of initiation, position and size. Tuber size is therefore partly determined before tuberization. Tuber-specific factors, such as the position and activity of the tuber initial also play a role, but the date of initiation of an individual tuber is not crucial. Later on, during tuber bulking, the activity of enzymes involved in starch metabolism, the levels of hormones, the mineral composition and the turgor potential are associated with the tuber growth rate. The growth characteristics of individual tubers may also vary since they are exposed to different conditions because of differences in position or growing period.     

Varietal and environmental effect on the glycoalkaloid content of potato (Solanum tuberosum L.)

The tubers of 14 potato varieties were analysed for glycoalkaloids. The level of glycoalkaloids in tubers of all the varieties analysed are within the safe limits for human consumption. The peels of tuber contained about 60–70% of the total glycoalkaloids present in the whole tuber. The levels of glycoalkaloids in leaves and tubers were correlated (r=0.865). There was a significant increase in the content of glycoalkaloids in peels of tubers exposed to sunlight. Glycoalkaloid contents increased at the rate of 1.9mg/100g fresh weight per day in peels of Kufri Jyoti tubers exposed to diffused sunlight.     

Effects of drying potatoes (Solanum tuberosum L.) after harvest on the incidence of disease after storage

Summary Drying potatoes for 2 weeks after harvest decreased the severity of skin spot, silver scurf, black dot and black scurf after 20–26 weeks' storage at 5°C. Diseases were decreased more after early than late harvest.     

Influence of bud position on the formation of tubers on multiple node cuttings of potato (Solanum tuberosum L.)

Summary Stem cuttings with variable number and position of leaves and variable number and age of buds were used to study the preference of tuberization at certain buds when several potential tuber sites were present. These cuttings were exposed to different orientations of the cuttings themselves and/or their light source. Inverting the cuttings did not affect overall tuberization, but had significant effects on individual buds, probably by the interaction between the original basipetal patterns in the cuttings and gravity effects. A smaller angle to the horizontal and lighting from below increased the overall expression of the tuberization stimulus. Leaf and bud position or age affected expression in a complicated manner, but the distance between screened bud and light-exposed leaf was crucial; buds most distant from illuminated leaves showed the strongest induction. The basic pattern of tuberization was basipetal.     

Effects of temperature and photoperiod on early growth and final number of tubers in potato (Solanum tuberosum L.)

Summary A phytotron experiment with all combinations of two photoperiod (12 or 18 h) and four temperature (18/12, 22/16, 26/20 or 30/24 °C; 12h/12h) treatments was carried out to analyse quantitative effects on early tuber growth, dry-matter partitioning and number of tubers of two cultivars. Higher temperatures and longer photoperiod delayed the onset of expolinear tuber growth and the onset of linear tuber bulking. Higher temperatures also gave lower absolute tuber growth rates. Photoperiod did not affect absolute tuber growth rate at lower temperatures. Higher temperature and longer photoperiod gave lower relative rates of partitioning of dry matter to the tubers. Differences between treatments in numbers of tubers initiated were inconsistent. This quantitative analysis of the effects of temperature and photoperiod on characteristics of tuber growth should prove useful in potato modelling.     

Effect of soil water tension and harvest time on levels of infestation byPhoma foveata Foister in soil and on tubers and stems of potato (Solanum tuberosum L.) in northern Sweden

Summary The incidence ofP. foveata in soil and tuber samples from field experiments often increased significantly as the harvest season progressed and temperatures fell. Mean levels of tuber and soil infestation were significantly higher in irrigated rows compared with those artificially sheltered from precipitation, both at normal and late harvest dates. Soil infestation was most frequently detected within 10 cm of the parent tuber. No pycnidia were visible on the haulm, and very few latently infected stem segments and leaves were detected using various methods. In three out of four years, the incidence ofP. foveata in tubers after wounding was reduced by washing them gently in tap water immediately after harvest.     

Suppression of marker genes in sequentially transformed potato (Solanum tuberosum L.)

Transgenic potato lines were developed from the advanced selection, ND860-2, by sequential transformation usingAgrobacterium tumefaciens and two different constructs. Five doubly-transformed lines were utilized to study gene expression of the first construct. Three doubly-transformed lines had less NPT II antigen than the singly-transformed counterpart from which each was derived. GUS activity was reduced in all doubly-transformed lines compared to their singly-transformed original genotypes. Therefore, NPT II and GUS genes in the first construct were suppressed to varying degrees by a second construct. Further investigations into this important subject will be necessary to enable multiple transformations with genes of agronomic interest.     

Plant regeneration from cell suspension culture of potato (Solanum tuberosum L.)

In the present study, for callus production leaf and stem segments of potato cultivar White Desiree were cultured on MS medium supplemented with 2,4-D, NAA and Kinetin (callus production medium). Calli then were transferred in the same liquid medium for cell suspension production. In the next step cell suspensions were transferred back to the callus production medium. Finally, calli derived from cell suspension were cultured on 6 different shoot initiation media (S1-S6). However, on S6 medium with combination of GA3 and BAP more than 80% of the calli produced shoot buds and shoots. Fully grown shoots then were rooted and produced whole plants. Chromosome and morphological analysis showed no somaclonal variation among regenerated plants.