首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 796 毫秒
1.
本文将30头杂交山羊分成3组,第1组(n=6)为对照组,第2组(n=12)静注大肠杆菌内毒素复制休克模型,第3组(n=12)在第2组基础上提前10min静注山莨菪碱性注射液,检测12h内不同时间血清丙二醛(MDA)含量,总SOD,Mn-SOD,CuZn-SOD,CAT和血液GSH-Px活性,结果表明,第2组血清MDA含量明显增加(P〈0.01),血清T-SOD,Mn-SOD,CuZn-SOD,GS  相似文献   

2.
山莨菪碱对内毒素诱导山羊弥漫性血管内凝血(DIC)的影响   总被引:3,自引:0,他引:3  
30头杂交山羊随机分成3组,第Ⅰ组(n=6)作为对照组;第Ⅱ组(n=12)静注大肠杆菌内毒素(1800EU/kg)两次,间隔24小时;第Ⅲ组(n=12)在第Ⅱ组处理基础上提前10分钟静注山莨菪碱(2.5mg/kg),于处理后第1、3、5、7、9、12小时分别检测血小板数、血浆纤维蛋白原含量、血浆凝血酶原时间(PT)、血浆凝血酶时间(TT)、副凝试验3P和EGT指标。结果表明:第Ⅱ组迅速发生DIC,表现血小板减少、纤维蛋白原含量下降、PT和TT延长、3P和EGT阳性(P<0.01);而第Ⅲ组血小板数在13小时明显高于第Ⅱ组,纤维蛋白原含量于第5小时后高于第Ⅱ组(P<0.01,P<0.05),PT和TT亦比第Ⅱ组显著缩短(P<0.01),3P和EGT的阳性检出率低于第Ⅱ组,结果提示应用山莨菪碱能明显抑制血小板减少,阻止DIC的发生  相似文献   

3.
山莨菪碱对内毒素诱导山羊肺脂质过氧化损伤的影响   总被引:5,自引:0,他引:5  
本文探讨内毒素诱导肺脂质过氧化损伤机制及山莨菪碱(654-2)对其保护作用。将36头杂交山羊随机分为3组。第1组作为对照,第Ⅱ组和第Ⅲ组静注大肠杆菌内毒素两次,间隔24h,第Ⅲ组于第二次注射内毒素前10min静注654-2,各组于5h,12h剖杀6头,检测肺组织抗氧化系统功能。结果表明:第Ⅱ,Ⅲ组肺组织MDA含量明显增加(P〈0.01),Ⅲ组肺组织MDA含量明显增加(P〈0.01),Ⅲ组显著低于Ⅱ  相似文献   

4.
本文通过给山羊静注大肠杆菌内毒素诱导内毒素休克,探讨内毒素休克时血液流变性的变化规律,并观察山莨菪碱(654-2)对其影响。结果表明,山羊内毒素休克时,低切率全血比粘度(LBV)和低切率全血还原比粘度(LBRV)明显升高(P<0.05),血浆比粘度(PV)和红细胞聚集指数(AI)均显著增高(P<0.01,P<0.05),红细胞变形能力(RCD)显著下降(P<0.05,P<0.01),当静注内毒素前10min给予654-2(2.5mg/kg)山羊的LBV和LBRV在1~5h显著高于对照组,5h前PV和AI值比对照组有明显升高,第7h后与对照组间无明显差异(P>0.05),且显著低于休克组(P<0.05),其RCD亦趋于正常。提示山羊内毒素休克时血液流变参数明显改变,血液粘度增加,红细胞聚集加剧,而应用654-2具有显著改变血液状态,缓解微循环障碍发生。  相似文献   

5.
内毒素休克山羊NO代谢的变化及山莨菪碱对其影响   总被引:3,自引:0,他引:3  
为了探讨一氧化氮(NO)在内毒素休克病理发生中的作用,将36头杂交山羊随机分成3组,每组12只,第Ⅰ组作为对照,第Ⅱ、Ⅲ组颈静脉注射大肠杆菌内毒素2次,间隔24h,第Ⅲ组于第2次注射内毒素前10min静脉注射山莨菪碱(654-2),检测血浆、肝、肾、肺组织中的NO水平和组织中一氧化氮合酶(NOS)活性。结果表明,第Ⅱ、Ⅲ组山羊第2次注射内毒素后,血浆NO水平明显高于第Ⅰ组(P<0.01),其中第Ⅱ组呈持续性升高,7h后第Ⅲ组显著低于第Ⅱ组(P<0.01);第Ⅱ、Ⅲ组肝、肾、肺组织中的NO含量和NOS活性均明显高于第Ⅰ组(P<0.01),12h后第Ⅲ组组织中的NO含量和NOS活性显著低于第Ⅱ组(P<0.01)。提示NO过量为内毒素休克时病理发生中关键的中介机制,预先给予654-2则能缓解休克时NO引起的毒性作用。  相似文献   

6.
采用 1,6二苯基1,3,5己三烯(1,6diphenyl1,3,5hexatriene, P H, Sigm a Chem ical Co)分子荧光偏振法研究了内毒素诱导山羊红细胞膜的流动性( P),膜脂区微粘度(η)及脂双层分子排列有序性系数( A)的变化及6542 的影响。结果表明, E T 处理组(Ⅱ组) E C M 的 P、和 A参数除在1h 高于对照组(Ⅰ组)和654 Z2处理(Ⅲ组)且差异不显著( P> 0.05)外,在 3,5,7,9,12h 均高于Ⅰ组和Ⅲ组( P<0.05), P< 0.01);Ⅲ组 P、和 A 参数在1 h低于Ⅰ组且差异不显著( P> 0.05),在3 h显著低于Ⅰ组( P< 0.01),在5 和7 h 高于Ⅰ组( P< 0.05, P< 0.01),在9 h,12 h 虽高于Ⅰ组但无显著差异( P> 0.05)。结果显示, E T 可使 E C M 荧光偏振度( P)、微粘度(η)和脂双层分子排列有序性( A)增加。膜流动性降低,而6542 能有效地改善 E T诱导的这些损伤变化,支持了6542 具有维护膜结构和功能的生理作用的理论。  相似文献   

7.
将41头产后40-100天卵巢静止奶牛分为四个处理组:OIUPMSG(n=12)、1000IUPMSG(n=12)、1250IUPMSG(n=12)及1500IUPMSG(n=5),处理后一周内的排卵率分别为0.0%、83.33%、91.67%及40.0%(P<0.01),从处理到妊娠间隔时间分别为89.1±36.7天、29.8±2且.7天、51.9±25.2天及60.2±29.6天(P<0.01);29头试验母牛又按产后40-60天及产后61-100天分为两组,处理后一周内的排卵率分别为92.9%及66.7%(P>0.05),从处理到妊娠间隔时间分别为50.3±27.0天及38.5±27.0天(P>0.05)。结果表明,PMSG处理卵巢静止母牛具有明显效果,其中以1000IU为最优,处理时间的早晚对处理效果无明显影响。  相似文献   

8.
山莨菪碱对山羊内毒素休克时肝脏自由基损伤的保护机理   总被引:15,自引:2,他引:13  
将36头杂交山羊随机分成3组:1组作为对照;Ⅱ组静注大肠力内毒素1800EU/kg,注射2次,间隔24h,Ⅲ组在同Ⅱ组处理基础上,于第2次注前10min静注山莨菪碱(654-2)注射液2.5mg/kg,第2次注射内毒素后5h和12h各组均剖杀6头山羊,检测肝脏抗氧化系统功能。结果表明:Ⅱ组肝组织丙二醛(MDA)含量明显高于Ⅰ组,谷胱甘肽过氧化物酶和谷胱甘肽不麦(GR)活性及总超氧化物岐化酶(T-S  相似文献   

9.
磷对奶牛红细胞膜脂质的影响   总被引:5,自引:0,他引:5  
对15例临床上有明显低磷血症的奶牛血清磷、红细胞膜脂质成分进行了测定。结果表明,低磷血症奶牛血清磷、红细胞膜磷脂明显降低;红细胞膜胆固醇及膜胆固醇/磷脂摩尔比值(nch/npl)明显升高,与健康对照组牛相比差异极显著(P<0.01);直线相关分析表明,血清磷与红细胞膜磷脂之间呈极显著正相关(r=0.917,y=10.852x+3.196,P<0.01);与红细胞膜胆固醇以及nch/npl之间分别呈显著负相关(r=-0.940,y=2.850x-1.072,P<0.01;r=-0.920,y=1.968x-1.401,P<0.01)。此外,红细胞膜微粘度与膜磷脂之间呈显著负相关(r=-0.954,y=19.122x-4.384,P<0.01),而与膜胆固醇以及nch/npl之间呈显著正相关(r=0.988,y=0.927x+0.964,P<0.01;r=0.978,y=-0.293x+1.113,P<0.01)。结果表明,磷是红细胞膜脂质成分改变的先决因素,而后者又是红细胞膜流动性及膜结构、功能发生变化并导致膜损伤的一个重要因素。  相似文献   

10.
24头太湖猪新生仔猪(二花×大白)分为四组,在出生后隔离并分别人工哺喂葡萄糖盐水(GS组,n=4),牛初乳(BC组,n=5),牛乳(BM组,n=6)48h和自然哺乳(PC组,n=9)。前3组在48h后回圈哺乳。初乳对新生仔猪血液蛋白电泳形态的影响,新生仔猪血清蛋白质浓度初生时平均为31.8±1.86mg/ml,PC组在出生后迅速增加,24h达81.78±8.6mg/ml此后略有下降。48h为56.67±11.36mg/ml,第7天为87.67±3.89mg/ml。BC和BM组与PC组有相似的变化;而GS组在48h内一直下降,48h为6.25±2.28mg/ml,自然哺乳后,第7天上升为47.75±2.68mg/ml。但BM、GS组在24h、48h和第7天血清总蛋白浓度显著低于PC组(P<0.01)。血清SDS—PAGE电泳显示,清蛋白(SA)PC、BC、BM三组48h内持续增加,GS组24h后显著下降,并且GS组在高分子量区(150kd)出现非抗体蛋白和多肽的聚合,其他三组则未见到。实验中未观察到牛乳酪蛋白向仔猪血液中的转移。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

13.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

14.
15.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

16.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

17.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

18.
19.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

20.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号