骨疏康对蛋鸡骨代谢的影响

为研究中药骨疏康对蛋鸡骨代谢的影响，选择90只58周龄的蛋鸡，随机分为3组。对照组饲喂基础日粮，骨疏康组在基础日粮中添加1％的骨疏康，依普拉芬组在基础日粮中添加25mg／kg的依普拉芬。试验为期90d。结果显示，与对照组比较，骨疏康组和依普拉芬组血清Ca、P、AKP、TRACP、BGP水平显著降低，肱骨、胫骨、股骨的骨指数、骨放射密度、皮质骨厚度、皮质骨面积、皮质骨面积比例以及血清中IGF-1水平显著升高，而骨疏康组与依普拉芬组之间无显著差异，表明骨疏康能显著抑制产蛋后期蛋鸡的骨吸收，促进骨形成，改善骨代谢。     

中药骨疏康对笼养蛋鸡生产性能的影响

54羽 2 0周龄伊莎蛋鸡分为 2组 ,A组为正常组 (2 7羽 ) ,B组为已发生骨质疏松组 (2 7羽 )。每组又随机分为 3组 ,每组 9只 ,分别喂骨疏康 1号、骨疏康 2号和对照组。试验期间 ,每天记录产蛋情况 ,定期测定骨矿含量、雌激素水平。结果表明 :骨疏康 1号能显著提高产蛋率 (P <0 0 5) ;骨疏康 2号在B组能显著提高产蛋率 ,在A组差异不显著 ,骨疏康 1号、 2号之间差异不显著。骨疏康 2号能显著提高蛋壳强度。中药对骨矿影响不显著。VitD3能显著提高产蛋率 ,改善蛋壳品质     

微生态制剂对蛋鸡产蛋性能和蛋品质的影响

本研究将3 000只海兰褐蛋鸡随机分成2组(对照组和试验组),对照组饲喂基础日粮,试验组在基础日粮中搅拌加入0.1%的微生态制剂,比较分析对照组与试验组之间平均耗料量、产蛋率、平均蛋重、不合格蛋率、蛋壳强度、蛋壳厚度、哈氏单位、蛋黄颜色等检测指标的差异性,以期掌握微生态制剂对蛋鸡产蛋性能和蛋品质的影响。试验结果显示,试验组的产蛋率、哈氏单位和蛋黄颜色均显著高于对照组,试验组的不合格蛋率显著低于对照组,试验组的平均日耗料量、平均蛋重、蛋壳强度和蛋壳厚度与对照组之间差异不显著。说明在蛋鸡基础日粮中添加微生态制剂对蛋鸡的日耗料量、蛋重、蛋壳强度和蛋壳厚度无显著影响,但能够显著提高蛋鸡的产蛋率,显著降低不合格蛋率,显著提高蛋鸡的产蛋性能和蛋品质。     

有机钙源对产蛋后期蛋鸡生产性能、蛋品质及骨钙含量的影响

文章旨在探讨不同有机钙水平日粮对产蛋后期蛋鸡生产性能、蛋品质及骨钙含量的影响。试验将65周龄、产蛋率一致的608只海兰褐壳蛋鸡随机分为4组，每组4个重复，每个重复38只。4组蛋鸡在为期8周的饲养试验期间以柠檬酸钙的形式饲喂钙水平为2.0%、2.5%、3.0%和3.5%的日粮。结果：与2%钙组相比，3.0%钙组蛋鸡的产蛋率显著提高4.51%（P＜0.05），破蛋率显著降低12.66%（P＜0.05）。3%钙组蛋鸡料蛋比较3.5%钙组显著降低3.65%（P＜0.05）。3.0%和3.5%钙组鸡蛋蛋黄颜色、蛋壳强度及储存7?d后哈夫单位显著高于2.0%钙组（P＜0.05），而2.0%钙组蛋壳厚度表现最低（P＜0.05）。3.0%和3.5%钙组胫骨强度较2.0%钙组分别显著提高7.03%和6.21%（P＜0.05），同时胫骨钙含量分别显著提高3.90%和4.30%（P＜0.05）。结论：在本试验条件下，产蛋后期蛋鸡日粮以柠檬酸钙的形式添加3.0%钙时可改善产蛋率和饲料效率，提高蛋壳厚度和蛋壳强度，进而降低破蛋率。 [关键词]钙|蛋鸡|生产性能|蛋品质|骨钙     

日粮中添加复合益生菌对蛋鸡生产性能及蛋品质的影响

为探究日粮中添加复合益生菌对蛋鸡生产性能及蛋品质的影响，本试验选用100羽42周龄蛋鸡，随机将它们分为4组，每组4个重复，每个重复5羽。对照组蛋鸡饲喂基础日粮，试验1组蛋鸡饲喂基础日粮+100mg/kg复合益生菌；试验2组蛋鸡饲喂基础日粮+150mg/kg复合益生菌；试验3组蛋鸡饲喂基础日粮+300 mg/kg复合益生菌。结果显示，与对照组蛋鸡相比，试验组蛋鸡产蛋率显著提高，料蛋比显著降低，此外，试验组蛋鸡所产鸡蛋的蛋壳重量、蛋壳强度、蛋壳厚度、蛋白高度及哈夫单位显著升高。试验表明，日粮中添加适量复合益生菌可提高蛋鸡生产性能，改善蛋品质。     

有机源锌和锰对蛋鸡生产性能、蛋壳和骨质量的影响

文章旨在研究日粮添加有机源锌和锰替代无机锌和无机锰对蛋鸡生产性能、蛋壳和骨骼质量的影响。试验选择31周龄产蛋性能接近的罗曼粉壳蛋鸡616只,随机分为7组,每组4个重复,每个重复22只。基础日粮锌和锰含量分别为50和30 mg/kg,对照组饲喂基础日粮,分别添加氧化锌和硫酸锰(锌40 mg/kg,锰60 mg/kg)。处理组分别在基础日粮中添加20 mg/kg有机锌,30 mg/kg有机锰、20 mg/kg有机锌+30 mg/kg有机锰、40 mg/kg有机锌、60 mg/kg有机锰、40 mg/kg有机锌+60 mg/kg有机锰,上述含量均以相应元素计。试验共进行7周。结果显示:日粮用有机锌或锰替代50%或100%无机锌、锰对蛋鸡生产性能相关参数均无显著影响(P> 0.05)。对照组相比,有机锌或锰替代无机锌或锰的比例对32、46、60和74周龄蛋鸡蛋壳相对重量、蛋壳密度和蛋壳厚度均无显著影响(P> 0.05)。与对照组相比,30 mg/kg有机锰、20 mg/kg有机锌+30 mg/kg有机锰组的蛋壳强度显著提高(P <0.05)。40 mg/kg有机锌+60 mg/kg有机锰组较无机锌、锰组显著提高了60、74周龄及全期蛋壳强度(P <0.05)。日粮添加有机锌或锰对胫骨物理参数及胫骨和趾骨灰分含量均无显著影响。结论 :日粮添加有机锌、锰替代无机锌锰对蛋鸡产蛋性能和骨质量无影响,但可以降低蛋鸡由于年龄升高对蛋壳强度的负面影响。     

有机微量矿物质对蛋鸡生产性能和蛋品质的影响

有机微量矿物质对蛋鸡的生产性能和蛋品质有重要作用，本试验选用42～46周龄蛋鸡160羽，随机分为4组，每组4个重复，每个重复10羽，对照组饲喂不添加有机微量矿物质的基础日粮，试验1、2、3组分别饲喂含有低、中、高浓度有机微量矿物质（锌、锰、铜）的日粮。结果显示，与对照组蛋鸡相比，试验组蛋鸡的日采食量、产蛋率、日产蛋量、平均蛋重以及料蛋比均没有显著变化，但蛋破损率显著降低，且与有机微量矿物质的添加量成正相关，此外，试验组的蛋壳重量、蛋壳强度以及蛋壳厚度显著提高（P <0.05）。试验表明，在蛋鸡日粮中添加有机微量矿物质可以降低蛋破损率，改善蛋品质，从而提高经济收益。     

日粮桑黄多糖对海兰褐蛋鸡生产性能、血清代谢指标和抗氧化能力的影响

试验旨在探讨桑黄多糖（PL-N）对海兰褐蛋鸡生产性能、血清代谢指标和抗氧化能力的影响。选取500只280日龄海兰褐蛋鸡，进行1周适应性饲养，随机分成4组，每组5个重复，每个重复25只，对照组（CK组）饲喂基础日粮，试验组分别在基础日粮中添加100、200、400 mg/kg PL-N，试验期30 d，测定生产性能、血清代谢和抗氧化能力。结果表明：与CK组相比，日粮中补充PL-N对蛋鸡的产蛋率、平均蛋重和料蛋比无显著影响，但降低了蛋鸡的破蛋率、增加了蛋壳厚度（P<0.05），蛋壳厚度、哈氏单位也随着日粮中PL-N含量的增加提高（P<0.05）；血清葡萄糖、甘油三酯、低密度脂蛋白胆固醇、总胆固醇含量随日粮中PL-N含量的增加呈线性降低（P<0.05），而高密度脂蛋白胆固醇含量增加，PL-N对血清总胆固醇、总蛋白、白蛋白、球蛋白的影响不显著，添加PL-N均可以增加蛋鸡血清中总抗氧化酶、过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性，丙二醛含量呈剂量依赖性降低（P<0.05）。可见，日粮中添加PL-N可改善海兰褐蛋鸡的蛋品质和机体的抗氧化能力，其可作为蛋鸡的潜在抗氧...     

日粮添加刺五加多糖对蛋鸡产蛋性能、蛋品质及血清生化指标的影响

文章旨在评估刺五加多糖对蛋鸡产蛋性能、蛋品质和血清生化指标的影响,试验将1200只360日龄的同批次海兰褐蛋鸡随机分成4组,每组5个重复,每个重复60只,对照组饲喂基础日粮,低量添加组饲喂基础日粮+400 mg/kg刺五加多糖,中量添加组饲喂基础日粮+800 mg/kg刺五加多糖,高量添加组饲喂基础日粮+1600 mg/kg刺五加多糖。结果表明,与对照组相比,中量添加组产蛋率、哈夫单位和蛋壳厚度显著提高（P <0.05）,料蛋比和血清甘油三脂显著降低（P <0.05）;与对照组相比,高量添加组哈夫单位显著提高（P <0.05）,甘油三酯显著降低（P <0.05）。可见饲料添加适量的刺五加多糖可以显著提高蛋鸡的产蛋率,降低料蛋比,提高鸡蛋的哈夫单位和蛋壳厚度,降低血清甘油三酯含量,从而提高蛋鸡的生产性能、鸡蛋品质,改善脂肪代谢。在本试验条件下,在蛋鸡饲料中添加800 mg/kg刺五加多糖较为适宜。     

混合型啤酒酵母粉对产蛋后期蛋鸡生产性能、 蛋品质和抗氧化的影响

试验旨在研究日粮中添加混合型啤酒酵母粉对产蛋后期蛋鸡生产性能、蛋品质和抗氧化的影响。试验选择60周龄海蓝褐蛋鸡6 000只,随机分成2个处理,每个处理5个重复,每个重复600只。对照组饲喂基础日粮,试验组在基础日粮中添加1.5 g/kg的混合型啤酒酵母粉。预试期3周,试验期8周。研究结果表明:①与对照组相比,日粮中添加混合型啤酒酵母粉显著提高了8 wk和5～8 wk蛋鸡的产蛋率和产蛋量(P<0.05),有提高4 wk蛋鸡产蛋量的趋势(P=0.083),显著降低了4、8、5～8 wk料蛋比和8 wk蛋鸡采食量(P<0.05),显著提高了5～8 wk和1～8 wk蛋鸡的合格蛋率(P<0.05)。②与对照组相比,日粮中添加混合型啤酒酵母粉显著提高了蛋鸡蛋白高度(P<0.05),有提高蛋壳强度的趋势(P=0.083),对蛋壳厚度、蛋黄颜色、哈氏单位、蛋黄比例、蛋清比例、蛋壳比例没有显著影响(P>0.05)。③与对照组相比,日粮中添加混合型啤酒酵母粉显著降低了蛋鸡肝脏和蛋黄MDA含量(P<0.05),显著提高了蛋黄SOD活性(P<0.05),对肝脏SOD活性没有显著影响(P>0.05)。综上所述,日粮中添加混合型啤酒酵母粉能够改善蛋鸡的产蛋性能、饲料效率、蛋品质和抗氧化性能。     

女贞子、五味子与寡糖配伍对肉鸡生产性能和免疫功能的影响

研究了中草药女贞子、五味子以及女贞子、五味子与葡萄糖甘露寡糖(MOS)配伍对肉鸡生产性能及免疫功能的影响。选择180只肉鸡公雏随机分为6组，空白组饲喂基础饲粮，抗生素组在基础饲粮中添加5mg／kg黄霉素，试验组分别在基础饲粮中添加1％女贞子、五味子、女贞子 MOS、五味子 MOS。试验从1日龄开始，试验期56d。结果表明：五味子 MOS在肉鸡生长中、后期能显著提高肉鸡的体增重；女贞子能提高肉鸡的ND抗体效价；各试验组均显著提高了鸡脾淋巴细胞在ConA诱导下的增殖反应。说明中草药添加剂能够提高肉鸡的体增重、增强肉鸡的免疫性能。     

Effects of dietary supplementation with ethoxyquin, magnesium oxide, methionine hydroxy analog, and B vitamins on tansy ragwort (Senecio jacobaea) toxicosis in beef cattle

Dried tansy ragwort containing pyrrolizidine alkaloids was fed as 2.5% of a complete (control) diet to Hereford steers, with and without (basal) a mixture of additives. The additives provided a dietary supplement equivalent to 0.1% ethoxyquin, 1% methionine hydroxy analog, 2% MgO, 2.7 mg of vitamin B6/kg of diet, 50 micrograms of vitamin B12/kg of diet, 0.45 g of folic acid/kg of diet, and 0.2 g of cobalt/kg of diet. The additives did not alter tansy ragwort toxicity substantially, as assessed by liver histologic changes, sulfobromophthalein clearance rate, and serum gamma-glutamyl transpeptidase activity. After 281 days, 1 of 4 steers fed the basal diet was alive, whereas 3 of 4 steers in the basal plus additives group were alive, suggesting some protective activity. The chronic lethal dose of tansy ragwort in steers was 3.6% of initial body weight.     

Effects of Source of Inorganic Phosphorus and Phytase Supplementation on Performance, Physiological Parameters and Bone Mineralization in Broilers

The objective of this study was to evaluate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1, 150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phosphorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2, 120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemented with 0, 250, 500, or 1,000 FTU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conversion as a result of feeding the different sources of inorganic phosphorus. The digestibility of phosphorus was significantly lower (P = 0.01) for chicks fed diets supplemented with tricalcium phosphate than for chicks fed the other two diets.  However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feed conversion showed a linear improvement (P = 0.03) with increasing levels of phytase inclusion (days 0 to 33).  Phytase supplementation resulted in linear increases in the digestibility of dry matter (P = 0.02), crude protein (P = 0.04) and energy (P < 0.01).  Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium (P = 0.05) and bone breaking strength (P = 0.04) than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets supplemented with dicalcium phosphate, tricalcium phosphate or defluorinated phosphate was similar and therefore production costs could be lowered by choosing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formulated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase supplementation.  However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be sufficient justification for the routine inclusion of phytase in diets fed to broilers.     

Effects of Source of Inorganic Phosphorus and Phytase Supplementation on Performance, Physiological Parameters and Bone Mineralization in Broilers

The objective of this study was to eval- uate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1,150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phos- phorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2,120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemen- ted with 0,250,500 ,or 1,000 P-＇rU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conver- sion as a result of feeding the different sources of in- organic phosphorus. The digestibility of phosphorus was significantly lower （P =0.01 ） for chicks fed di- ets supplemented with tricalcium phosphate than for chicks fed the other two diets. However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feedconversion showed a linear improvement （P = 0.03 ） with increasing levels of phytase inclusion （ days 0 to 33 ）. Phytase supplementation resulted in linear increa- ses in the digestibility of dry matter （P = 0.02 ）, crude protein （ P --- 0.04 ） and energy （ P 〈 0.01 ）. Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium （ P = 0.05 ） and bone breaking strength （P = 0.04 ） than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets sup- plemented with dicalcium phosphate, tricalcium phos- phate or defluorinated phosphate was similar and therefore production costs could be lowered by choo- sing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formu- lated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase sup- plementation. However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be suf- ficient justification for the routine inclusion of phytase in diets fed to broilers.     

中草药与寡糖配伍对肉鸡抗氧化功能的影响

试验研究了中草药女贞子、五味子以及女贞子、五味子与葡萄糖甘露寡糖(MOS)配伍对肉鸡抗氧化指标的影响。选择180只1日龄肉鸡公雏随机分为6组,对照组饲喂基础饲粮,抗生素组在基础饲粮中添加5mg/kg黄霉素,试验组分别在基础饲粮中添加1%女贞子、1%五味子、1%女贞子+0.5%MOS、1%五味子+0.5%MOS。试验从1日龄开始,试验期56d。结果显示:女贞子、五味子可提高肉鸡心脏超氧化物歧化酶(SOD)活性(P<0.05),以及血清和组织谷胱甘肽还原酶(GR)活性(P<0.05),降低血清和心脏丙二醛(MDA)含量(P<0.05)。上述试验结果表明中草药添加剂能够提高肉鸡抗氧化功能。     

微生物植酸酶对猪钙、磷利用率及骨骼发育的影响

本试验旨在研究从断奶到肥育结束,在饲粮中添加微生物植酸酶对猪钙、磷代谢及骨骼发育的影响。试验采用完全随机设计,将72头35日龄断奶的仔猪分为4组,每组3个重复,每个重复6头。试验在相同钙水平基础上设4个组,分别为:1)玉米-豆粕基础日粮(对照组);2)基础日粮 植酸酶-50%磷酸氢钙(处理1);3)基础日粮 植酸酶-75%磷酸氢钙(处理2);4)基础日粮 植酸酶-100%磷酸氢钙(处理3)。试验期按体重分3个阶段:8~20 kg、20~50 kg、50~90 kg,3个阶段植酸酶的添加量分别为750、500和250 U/kg。试验结果表明:(1)在8~20 kg和20~50 kg阶段,添加植酸酶的3个处理,钙、磷消化率显著高于对照组(P<0.05);血钙和血磷浓度,处理1和处理2与对照组无显著差异(P>0.05),处理3显著低于对照组(P<0.05)。在50~90 kg阶段,添加250 U/kg植酸酶代替100%磷酸氢钙,血磷、血钙浓度显著低于对照组(P<0.05);(2)在试验的3个阶段添加植酸酶同时降低饲粮无机磷水平,对血清碱性磷酸酶活性均无显著影响(P>0.05);(3)添加植酸酶使胃中植酸磷消化率显著提高(P<0.0 1),粪中磷排出量显著降低(8~2 0 kg、2 0~5 0 kg阶段,P<0.0 1;5 0~9 0 kg阶段,P<0.0 5);(4)添加植酸酶代替50%或75%磷酸氢钙,猪的掌骨灰分与采食正常磷日粮的对照组无显著差异,但代替100%磷酸氢钙组,猪的掌骨灰分和蹠骨强度则显著低于对照组(P<0.05);添加植酸酶代替50%磷酸氢钙组,猪的蹠骨强度显著高于对照组(P<0.05),代替75%磷酸氢钙组与对照组差异不显著(P>0.05)。总之,在断奶和生长阶段,在猪玉米-豆粕日粮中添加植酸酶可代替部分磷酸氢钙,促进了钙、磷消化利用,促进了骨骼生长,也促进了植酸磷的利用,降低了粪磷排出。     

Relative bioavailability of phosphorus in inorganic phosphorus sources fed to growing pigs

The relative bioavailability of P in 5 sources of inorganic P was determined using growing pigs. The 5 sources of inorganic P were dicalcium phosphate (DCP), monocalcium phosphate (MCP) containing 50% MCP (MCP50), MCP containing 70% MCP (MCP70), MCP containing 100% MCP (MCP100), and monosodium phosphate (MSP). A total of 11 diets were formulated. The basal diet was formulated to contain 0.10% P, and 10 additional diets were formulated by adding 0.07 or 0.14% P from each of the 5 P sources to the basal diet. Growing pigs (n = 44; initial BW: 16.8 ± 4.3 kg) were individually housed and randomly allotted to the 11 experimental diets. Feed was provided on an ad libitum basis throughout the 28-d experimental period. At the conclusion of the experiment, all pigs were killed, and 4 bones (i.e., the third and fourth metacarpals on both front feet) were harvested. Bone-breaking strength, bone ash, and Ca and P concentrations were determined. The concentration of bone ash increased (P < 0.05) as MCP50, MCP70, MCP100, or MSP were added to the basal diet, and the concentration of bone P also increased (P < 0.05) as MCP70, MCP100, or MSP were added to the basal diet. The relative bioavailability of P in each of the feed phosphates was determined using slope ratio methodologies based on breaking strength, and expressed relative to MSP. The slope of the regression line for diets containing MSP or MCP100 was steeper (P < 0.05) than the slope for pigs fed the diet containing DCP, but not different (P > 0.05) from that of pigs fed diets supplemented with MCP50 or MCP70. In conclusion, P in MSP and MCP100 is more bioavailable than P in DCP, but there were no differences within MCP sources.     

酵母培养物对肉鸡机体抗氧化和胆固醇代谢的影响

210只1日龄艾维茵肉仔鸡,随机分成7组,分别为对照组、0.1%、0.3%、0.5%、0.7%、0.9%酵母培养物组和抗生素组（5 mg/kg）,每组设3个重复,每个重复10只鸡,试验期28 d,研究不同添加水平的酵母培养物和抗生素对肉鸡机体抗氧化和胆固醇代谢的影响。结果表明,0.5%的酵母培养物组显著降低了肉鸡肠道的氨浓度,与对照组和抗生素组相比,分别降低15.49%和18.83%（P<0.05）;0.5%酵母培养物组提高肉鸡的超氧化物歧化酶（SOD）水平,与对照组和抗生素组相比提高了33.52%和28.26%（P>0.05）;0.5%酵母培养物组的肌肉总胆固醇含量最低,较对照组和抗生素组分别降低26.47%和20.89%,因此,酵母培养物有提高肉鸡机体抗氧化功能和降低血清、肌肉总胆固醇含量的趋势。     

Evaluation of biochemical characters of broiler chickens during dietary aflatoxin (50 and 100 ppb) and clinoptilolite exposure

Aflatoxin (AF) and clinoptilolite (CLI, a natural zeolite) were added to broiler food and some biochemical values and enzyme activities were evaluated. The experimental design consisted of six dietary treatments. (1) CONTROL: basal diet; (2) CLI: basal diet plus 15 g clinoptilolite kg(-1) diet; (3) 50 ppb AF: basal diet plus 50 microg total aflatoxin (AF; the composition given below) kg(-1) diet; (4) 50 ppb AF+CLI: basal diet plus 50 microg AF plus 15 g CLI kg(-1) diet; (5) 100 ppb AF: basal diet plus 100 microg AF kg(-1) diet; (6) 100 ppb AF+CLI: basal diet plus 100 microg AF plus 15 g CLI kg(-1) diet. A commercially available CLI was provided from the west region of Turkey and its chemical formula is "KNa(2)Ca(2)(Si(29)AL(7))O.7(2).32H(2)O". For this a total of 576 1-day-old Ross broiler chicks were housed in six treatment groups from days 1 to 42. AF treatment significantly increased the serum Na levels and the aspartate-amino-transferase (ASAT) and alanine-amino-transferase (ALAT) enzyme activities, while total protein, albumin, total cholesterol uric acid, and K levels were not significantly different between groups. These results suggest that these low AF levels in food did not change the serum biochemistry but significantly affected the enzyme activities in broilers.