丰容对圈养东黑白疣猴行为的影响

笔者对红山森林动物园灵长2馆和3馆现有的1只成年雄性东黑白疣猴、4只成年雌性东黑白疣猴、3只亚成年东黑白疣猴(2015年繁殖的3只亚成年疣猴均为雌性,2016年繁殖的2只亚成年疣猴为1雌1雄未做观察)进行行为观察。为了提高动物福利,为野生动物提供优良的生活环境,最大限度地利用笼舍,笔者对疣猴的笼舍进行了丰容。本文对此次丰容进行了介绍、探讨和总结,希望对相关圈养灵长类动物的丰容提供帮助。     

圈养环境下黑帽悬猴行为时间分配和活动节律

2017年4月在南京红山森林动物园,采用瞬时扫描取样法观察5只黑帽悬猴(Cebus apella)的行为,分析不同性别、年龄组的日行为时间分配和活动节律。结果表明,圈养黑帽悬猴的主要行为活动为休息(52. 06±1. 03)%、移动(23. 22±0. 58)%、取食(14. 65±0. 69)%、理毛(4. 98±0. 35)%等。雄猴和雌猴的休息、玩耍、乞食和刻板行为所占时间比例差异显著。相比于成年黑帽悬猴,幼猴玩耍的时间更长(χ~2=13. 643,P <0. 05),乞食行为所占时间更多(χ~2=4. 057,P <0. 05)。雄猴取食时间先于雌猴和幼猴,在上午和下午投食后均优先取食。周末游客人数比平日显著增加,雌猴和幼猴乞食行为也显著增多,其波动节律具有一致性。针对圈养黑帽悬猴行为管理提出了一些丰容措施和游客管理建议。     

成年黄山短尾猴春季昼间行为时间分配

2007年3～5月,采用焦点动物取样法对黄山野生猴谷YA1群黄山短尾猴成年个体行为时间分配进行系统观察.数据分析显示:黄山短尾猴成年个体日活动行为主要为休息(59.72%)、理毛(17.50%)和觅食(14.82%),其他行为如漫游(5.86%)、玩耍(0.04%)和其他(1.64%)时间花费较少,觅食高峰与投喂时间基本一致.老年组个体比成年组个体花费较多的时间用于休息(Z=-5.811,P＜0.01)和理毛(Z=-4.913,P＜0.01),较少时间漫游(Z=-3.989,P＜0.01).雄性个体花费较多时间休息(Z=-3.577,P＜0.01)而雌性个体花费大量时间进行理毛(Z=-3.134,P＜0.01).哺乳期雌性漫游行为(Z=-2.9541,P＜0.01)低于非哺乳期雌性个体,其他行为(Z=-2.9541,P＜0.01)明显高于非哺乳期雌性,而觅食行为(Z=-0.1773,P＞0.05)无显著差异性.结果表明人工投喂是导致其行为时间分配的主要原因.     

人工繁育黔金丝猴幼子行为发育研究

黔金丝猴是中国Ⅰ级保护野生动物,为保护这一濒危物种,2013年4月～2014年4月,在北京动物园对2013年4月出生的1只雄性黔金丝猴的行为发育进行了研究.利用行为取样法和连续记录法记录1周岁内婴猴首次出现的行为和婴猴和父亲玩耍的时间以及与母亲接触的时间.结果表明：2日龄有抓网动作且力度不小,脖子能挺立支撑头部,睁大眼睛转头看东西;11日龄能吸吮伸到嘴边的橙子,14日龄能离开母亲自己爬网,64日龄能真正吞咽食物,3月龄时婴猴的行为已基本完善.3月龄后逐渐出现玩耍、梳理、攻击、警戒等行为.雄婴关系与年龄存在正相关,6月龄后基本稳定.幼子和公猴玩耍时间表明2月龄时开始与父亲有少量接触,以后逐渐增加,到6月龄时增加到13.09％,之后一直到周岁,婴儿被父亲完全接纳.和母猴相处时间为头1个月婴猴主要呆在母亲怀里,到3月龄后与母亲相处时间一直稳定维持在20％ ～ 30％的水平上.     

冬季笼养和散养幼龄和成年母兔的维持行为分析

2011年1月到2月,在哈尔滨香坊实验农场,对舍内笼养和舍外散养母兔(Rabbit)越冬期日行为及活动时间分配进行观察研究,结果表明,笼养母兔主要以趴卧为主,散养母兔主要以运动为主;笼养母兔成年和幼年的取食、修饰和趴卧行为差异性显著,其他行为无明显的差异性,散养母兔成年和幼年各行为均无明显的差异性;笼养和散养的母兔行为相关性不同。     

大自然的瑰宝--秦岭金丝猴

金丝猴的社会是一夫多妻式的母系社会。每一个猴群都是由若干个社会单元(我们许多学者称之为“家庭单元”)与一个全雄单元组成,社会单元内有一只成年雄猴以及他的妻室们——多个成年雌猴、亚成年雌     

恒河猴血液细胞、生化和凝血功能正常参考指标的测定

测定分析人工饲养及在非麻醉(清醒)状态下108只健康成年恒河猴的血常规、血液生化及血小板和凝血功能正常值,并分析各项检测数据的性别差异。血常规测定值17项,其中,雌猴较雄猴极显著高(P<0·01)的项目为红细胞分布宽度(RDW)。血液生化测定值17项,其中,雌猴比雄猴显著低(P<0·05)的项目有丙氨酸转氨酶(ALT),显著高(P<0·05)的项目有碱性磷酸酶(ALP)。血小板和凝血功能测定值9项,其中,雌猴比雄猴明显高(P<0·05)的项目为活化部分凝血活酶时间(APTT),极显著高(P<0.01)的项目为凝血酶时间(TT)。建立了人工饲养条件下健康成年恒河猴的血常规、血液生化、血小板和凝血功能正常参考值指标,丰富和完善了恒河猴动物实验和疾病诊断中血液分析的基础知识。     

峨眉山藏猕猴与游客的人猴关系

为了探讨峨眉山藏猕猴与游客接触相互行为与猴和游客年龄/性别组间的关系,以及猴群对游客的攻击行为,2010年4月13～18日和9月24日～10月5日共16 d以峨眉山一线天生态猴区藏猕猴与游客为研究对象进行观察。结果表明：青年猴与未成年、20～30岁、30～50岁和50岁以上各年龄阶段的女性游客接触行为比例最高,均占一半以上,分别为62.5%、57.82%、56.12%、52%,且藏猕猴与不同性别、年龄的游客接触相互行为变化差异极显著（P〈0.01）。藏猕猴主动接触游客的行为比例（74.35%）明显高于人主动投食而引起的接触相互行为比例（25.65%）,藏猕猴攻击游人的行为比例（10.02%）远远低于接触相互行为比例（89.98%）,而且峨眉山藏猕猴与不同性别、年龄的游客攻击行为变化差异极显著,其中未成年猴和成年雄猴攻击性较强。     

笼养灰鹤繁殖期行为时间分配的研究

2006年5月1日～7月18日,对哈尔滨北方森林动物园1对笼养灰鹤繁殖期行为进行观察,研究表明：笼养条件下,灰鹤静立（雄31．08％,雌35．81％）、理羽（雄19．81％,雌12．45％）、采食（雄13．23％,雌15．09％）行为比例较高,而繁殖行为占总行为时间比例雄鹤为2．49％、雌鹤为10．30％。灰鹤各行为日节律不明显;静立、理羽、休息和繁殖行为的发生受天气状况影响显著,尤其是静立和理羽差异极显著。灰鹤雌雄行为差异性在中期较显著,就整个繁殖期看,受天气状况的影响行为差异不显著。     

桑天牛寄生性天敌花绒寄甲成虫的日活动节律

花绒寄甲(Dastarcus helophoroides Fairmaire)是桑天牛等林木蛀干害虫重要的天敌昆虫,明确其雌、雄成虫的日活动行为节律,可为人工饲养和生物防治应用等提供理论依据。采用人工气候箱(温度22℃、RH 60%±10%)单头隔离饲养花绒寄甲成虫,对其移动、取食、饮水、木块处静息、木块外静息5种活动行为的日节律进行观察。各种活动行为的日时间分配:以木块处静息最多(63.39%),饮水行为最少(1.45%),各行为活动的日时间分配存在极显著差异。雌、雄成虫各活动行为的高峰时间:移动行为雌、雄成虫均出现在21:30;取食行为雌成虫在0:00,雄成虫在8:30和19:30;饮水行为雄成虫在6:00—6:30,雌成虫在8:30;木块处静息行为雌成虫在6:30,雄成虫在19:30;木块外静息行为雌成虫在10:00,雄成虫在10:00和13:30—14:30。雌、雄成虫各种活动行为在全天的时间分配:雌、雄成虫的移动、木块外静息及雌成虫的取食行为存在极显著差异,雌、雄成虫饮水和木块处静息及雄成虫取食行为均无显著性差异。雌、雄成虫各种活动行为在白天和夜晚时段的分配:雌雄成虫移动、雄成虫木块外静息行为存在极显著性差异,雌成虫木块外静息行为存在显著性差异,其他行为均无显著性差异。在上午、中午、下午和夜晚4个时间段的分配:雌、雄成虫的移动行为均存在极显著性差异,雌成虫取食及木块外静息行为存在显著性差异,其他行为均无显著性差异;4个时间段雌、雄成虫之间仅夜晚在木块处静息存在显著性差异。以上结果显示,花绒寄甲成虫活动行为受到明显的时间影响,白天大多数时间处于静息状态,夜晚20:00至次日5:00处于活跃期;各种活动行为日节律在雌、雄成虫之间的差异不显著。     

Personality and behavioral changes in Asian elephants (Elephas maximus) following the death of herd members

Elephants are highly social beings with complex individual personalities. We know that elephants have a general interest in death, investigating carcasses, not just limited to kin; however, research does not explore in depth whether individuals change their behavior or personality following traumatic events, such as the death of a conspecific. Within a captive herd of Asian elephants (Elephas maximus) housed at Chester Zoo, UK, we measured social behavior and proximity and personality using the Ten-Item Personality Inventory, and found age-related and relationship-related changes in both behavior and personality following the deaths of herd members. Overall, the herd spent less time socializing and engaging in affiliative behaviors following the death of the adult female when compared to baseline data, yet spent more time engaging in these behaviors after the death of two calves. The death of the central female had a dramatic impact on her infant calf, resulting in increasingly withdrawn behavior, yet had the opposite effect on her adult daughter, who subsequently established a more integrated role within the herd. Emotional Stability fell in the motherless calf but rose in an adult female, who had lost her adult daughter, but had a new calf to care for. We suggest that the greater impact on the behavior and personality of surviving herd members following the deaths of calves, compared to an adult member, attests to the significance of the unifying role played by calves within an elephant herd.     

Small intestinal volvulus in a free-ranging female dugong (Dugong dugon)

An adult female dugong (Dugong dugon) was found dead and floating in Moreton Bay, Queensland, Australia. This animal was found to have a 360° mesenteric volvulus with infarction of the associated segment of small intestine, and fibrinous peritonitis. Mortality was attributed to the volvulus and its sequelae. The cause was not apparent on gross or histological examination.     

肉用型牛新品种——辽育白牛的培育

辽育白牛是以夏洛来牛为父本,以辽宁本地黄牛为母本级进杂交后,形成含夏洛来牛93.75%、本地牛6.25%血统,适应当地气候和饲养条件的肉牛新群体。初生重平均：公犊43.6kg,母犊40.3 kg。成年体重：种公牛平均1 084 kg,母牛平均为497 kg,其BPI（肉用指数）分别为7.3和3.9 kg/cm,达到肉用型牛BPI标准。目前育种基础群共有辽育白母牛4 200余头,核心群共有1 100余头,制冻精种公牛9头。辽育白牛共8个家系。外貌整齐,遗传性能稳定,具有生长快、产肉多、耐粗饲、繁殖力良好等优点。     

Selection of foot-and-mouth disease viruses by passage in bovine and swine kidney cell cultures

Two uncloned populations of foot-and-mouth disease virus, one pathogenic for adult mice and the other nonpathogenic, were passaged in cultures of primary bovine kidney (BK) cells and a line of pig kidney (MVPK) cells. Within 10 passages in MVPK cells, the nonpathogenic virus became pathogenic for adult mice, but similar passages of this virus in BK cells did not affect its pathogenicity. In contrast, passage of the pathogenic virus in MVPK cells resulted in a decrease in pathogenicity, but again passage in BK cells had no effect on this characteristics. Neither of the viruses changed in pathogenicity for infant mice during the four passages that were tested. The nonpathogenic virus passaged in BK cells was more infectious for BK than for MVPK cells, but after passage in MVPK cells, this virus was about equally infectious for the two types of cells. Infectivity of the pathogenic virus was relatively unchanged by passage in either type of cell. The parent nonpathogenic virus and the 10th BK cell passage of this virus were much more resistant to adsorption with homogenized mouse kidney than were the MVPK cell passages of nonpathogenic virus. The parent and passaged pathogenic viruses were readily adsorbed. The results demonstrated that passage of the two viruses in MVPK cells had a pronounced selective effect.     

Sarcocystosis in a Barasingha deer (Cervus duvauceli branderi).

An adult female hard-ground swamp deer (Barasingha), Cervus duvauceli branderi, was found dead of unknown cause in Kanha National Park. A necropsy of this animal failed to reveal any significant gross lesions. However, histopathologic examination revealed mature sarcocysts of Sarcocystis in the cardiac muscles. The morphology of the sarcocysts was similar to that of different species of Sarcocystis from wild and domestic animals. Final identification of Sarcocystis in this case could not be made. This is the first recorded case of Sarcocystis infection in hard-ground Barasingha deer.     

Toxoplasma gondii in an African crested porcupine (Hystrix cristata).

An adult female crested porcupine (Hystrix cristata) was evaluated for acute onset of neurologic signs including head tilt, circling, and ataxia. She was found dead in her holding area 2 days after initially exhibiting clinical signs. Necropsy was unremarkable. Histopathology of brain tissue revealed the presence of protozoal cysts associated with inflammation as the underlying cause of clinical signs and death. Immunohistochemical staining of brain tissue for Toxoplasma gondii was strongly positive. PCR on fresh brain confirmed T. gondii as the causative organism. An adult male in the same enclosure has demonstrated similar neurologic signs over the past 3 years and has failed to respond to various medical treatments. Clinical disease associated with T. gondii has not been previously reported in this porcupine species or any other Old World porcupines, although there are several reports of clinical toxoplasmosis involving New World porcupine species.     

环境丰容对单独圈养黑猩猩行为影响的研究

对广州动物园科研中心的2只单独圈养的黑猩猩进行环境丰容,采取焦点观察法和连续记录法,记录2只黑猩猩丰容前后的各行为持续时间。结果表明,环境丰容对2只单独圈养黑猩猩的行为产生影响,雌性黑猩猩积极行为显著增加,刻板行为和一般行为显著降低,而雄性黑猩猩的探究和进食行为明显提高。     

Characterization of a factor from bovine intestine that protects against Cryptosporidium parvum infection

Cryptosporidium parvum is a protozoan parasite that causes intestinal infection in a variety of mammals. We have previously described a factor in adult rat or adult bovine intestinal mucosa that protects against C. parvum infection when fed to susceptible infant rats. This factor is absent in intestinal mucosa from bovine calves. In the present study we describe the further characterization of the active component of bovine intestinal mucosa. The ability to protect infant rats against C. parvum infection was found to be associated with the extrinsic membrane protein fraction of the intestinal mucosa. Extrinsic membrane preparations from adult cows, adult rats, and calves were separated by SDS-PAGE. A band with apparent molecular mass of 54 kDa was seen in preparations from adult rat and cow, but not calf. Protein was transferred to PVDF membrane and from this the band was excised and subjected to N-terminal sequence analysis using a gas-phase protein sequenator. A 15-amino acid consensus sequence was generated with homology to leucine aminopeptidase (LAP). Purified LAP was purchased from a commercial source and tested for ability to protect infant rats against C. parvum infection. Rats fed LAP from 7 to 11 days of age and challenged with C. parvum at 9 days were significantly less infected than controls upon necropsy at 15 days of age. These data suggest that a protein with N-terminal sequence homology to LAP may reduce susceptibility of infant rats to C. parvum infection.     

Dispharynx nasuta (Nematoda:Acuariidae) infection causing proventricular lesions and death in three captive rosellas (Psittaciformes:Psittacidae)

Two adult male Crimson rosellas (Platycercus elegans) and an adult female eastern rosella (Platycercus eximius) were found dead in their cages in a private aviary in March 2009, April 2010, and February 2010, respectively, without premonitory signs. Their postmortem examination showed pectoral muscle atrophy and a distended proventriculus. The proventricular mucosal surface was covered with a gelatinous, whitish material, where a large number of nematode parasites identified as Dispharynx nasuta were present. A histologic examination revealed a hyperplastic mucosa, spirurid nematodes in the mucosal layer and inflammatory mononuclear cells in the lamina propria. This is the first report of a D. nasuta infection associated with proliferative proventriculitis and subsequent death in psittacine birds.     

Acute toxoplasmosis in three wild arctic foxes (Alopex lagopus) from Svalbard; one with co-infections of Salmonella Enteritidis PT1 and Yersinia pseudotuberculosis serotype 2b

Acute disseminated toxoplasmosis was diagnosed in three wild arctic foxes (Alopex lagopus) that were found dead in the same locality on Svalbard (Norway). The animals included one adult female and two 4-months-old pups. The adult fox was severely jaundiced. Necropsy revealed multifocal, acute, necrotizing hepatitis, acute interstitial pneumonia, and scattered foci of brain gliosis, often associated with Toxoplasma tachyzoites. One pup also had Toxoplasma-associated meningitis. In addition, the latter animal was infected with Yersinia pseudotuberculosis serotype 2b and Salmonella Enteritidis phage type 1 (PT1), which may have contributed to the severity of the Toxoplasma infection in this animal. The diagnosis of toxoplasmosis was confirmed by positive immunohistochemistry and detection of anti-Toxoplasma gondii antibodies in serum of all foxes. The animals were negative for Neospora caninum, canine distemper virus, canine adenovirus, and rabies virus on immunolabelling of tissue sections and smears.