Interannual Herbaceous Biomass Response to Increasing Honey Mesquite Cover on Two Soils

This study quantified herbaceous biomass responses to increases in honey mesquite (Prosopis glandulosa Torr.) cover on two soils from 1995 to 2001 in north central Texas. Vegetation was sampled randomly with levels of mesquite ranging from 0% to 100%. With no mesquite covering the silt loam soils of bottomland sites, peak herbaceous biomass averaged (±SE) 3 300 ± 210 kg · ha⁻¹ vs. 2 560 ± 190 kg · ha⁻¹ on clay loam soils of upland sites (P = 0.001). A linear decline of 14 ± 2.5 kg · ha⁻¹ in herbaceous biomass occurred for each percent increase in mesquite cover (P = 0.001). The slope of this decline was similar between soils (P = 0.135). Herbaceous biomass with increasing mesquite cover varied between years (P = 0.001) as did the slope of decline (P = 0.001). Warm-season herbaceous biomass decreased linearly with increasing mesquite cover averaging a 73 ± 15% reduction at 100% mesquite cover (P = 0.001) compared to 0% mesquite cover. Cool-season herbaceous biomass was similar between soils with no mesquite, 1 070 ± 144 kg · ha⁻¹ for silt loam vs. 930 ± 140 kg · ha⁻¹ for clay loam soils, but averaged 340 ± 174 kg · ha⁻¹ more on silt loam than on clay loam soils at 100% mesquite cover (P = 0.004). Multiple regression analysis indicated that each centimeter of precipitation received from the previous October through the current September produced herbaceous biomass of 51 kg · ha⁻¹ on silt loam and 41 kg · ha⁻¹ on clay loam soils. Herbaceous biomass decreased proportionally with increasing mesquite cover up to 29 kg · ha⁻¹ at 100% mesquite cover for each centimeter of precipitation received from January through September. Increasing mesquite cover reduces livestock forage productivity and intensifies drought effects by increasing annual herbaceous biomass variability. From a forage production perspective there is little advantage to having mesquite present.     

Spatial Redistribution of Nitrogen by Cattle in Semiarid Rangeland

Nitrogen (N) availability can strongly influence forage quality and the capacity for semiarid rangelands to respond to increasing atmospheric CO₂. Although many pathways of nitrogen input and loss from rangelands have been carefully quantified, cattle-mediated N losses are often poorly understood. We used measurements of cattle N consumption rate, weight gains, and spatial distribution in shortgrass rangeland of northeastern Colorado to evaluate the influence of cattle on rangeland N balance. Specifically, we estimated annual rates of N loss via cattle weight gains and spatial redistribution of N into pasture corners and areas near water tanks, and used previous studies to calculate ammonia volatilization from urine patches. Using measurements of plant biomass and N content inside and outside grazing cages over 13 yr, we estimate that cattle stocked at 0.65 animal unit months (AUM) · ha^?1 consumed 3.34 kg N · ha^?1 · yr^?1. Using an independent animal-based method, we estimate that cattle consumed 3.58 kg N · ha^?1 · yr^?1 for the same stocking rate and years. A global positioning system tracking study revealed that cattle spent an average of 27% of their time in pasture corners or adjacent to water tanks, even though these areas represented only 2.5% of pasture area. Based on these measurements, we estimate that cattle stocked at 0.65 AUM · ha^?1 during the summer can remove 0.60 kg N · ha^?1 in cattle biomass gain and spatially redistribute 0.73 kg N · ha^?1 to areas near corners and water tanks. An additional 0.17 kg N · ha^?1 can be lost as NH₃ volatilization from urine patches. Cumulatively, these cattle-mediated pathways (1.50 kg N · ha^?1) may explain the imbalance between current estimates of atmospheric inputs and trace gas losses. While NO_x emission remains the largest pathway of N loss, spatial N redistribution by cattle and N removed in cattle biomass are the second and third largest losses, respectively. Management of cattle-mediated N fluxes should be recognized as one means to influence long-term sustainability of semiarid rangelands.     

Age and Body Condition of Goats Influence Consumption of Juniper and Monoterpene-Treated Feed

Redberry juniper (Juniperus pinchotii Sudworth) is an invasive, evergreen tree that is rapidly expanding throughout western and central Texas. Goats will consume some juniper on rangelands; however, intake is limited. The objective of our research was to determine how the age and body condition of goats influence their consumption of juniper and an artificial feed containing 4 monoterpenes. Two separate experiments were conducted. Experiment 1 examined the intake of redberry juniper foliage and used 39 goats either young (2 yr) or mature (> 6 yr). One-half of each age group was fed appropriate basal rations to reach either a high (HBC) or low body condition (LBC). Goats in LBC ate more (P < 0.01, 8.6 g · kg⁻¹ body weight [BW] ± 0.7 SE) juniper than those in HBC (2.3 g · kg⁻¹ BW ± 0.3 SE), and young animals consumed more (P < 0.05, 7.2 g · kg⁻¹ BW ± 0.7 SE) juniper than mature goats (3.9 g · kg⁻¹ BW ± 0.5 SE) across body condition treatments. In experiment 2, 36 goats, either young (2 yr) or mature (> 6 yr) and in either HBC or LBC, were offered a synthetic ration treated with 20.8 g · kg⁻¹ of 4 monoterpenes found in redberry juniper. Goats in LBC ate more (P < 0.01, 25.3 g · kg⁻¹ BW ± 1.0 SE) of the terpene-treated feed than those in HBC (17.5 g · kg⁻¹ BW ± 0.7 SE), and young animals ate more (P < 0.05, 22.5 g · kg⁻¹ BW ± 0.8 SE) than mature goats (20.3 g · kg⁻¹ BW ± 0.8 SE) across body condition treatments. Total intake as a proportion of body weight was also affected by body condition. Age and body condition are important factors that influence intake of chemically defended plants. A better understanding of how these attributes affect diet selection will aid livestock producers in improving grazing management.     

Growth of Chickasaw Plum in Oklahoma

Management of rangelands for wildlife and livestock entails understanding growth of clonal shrubs such as Chickasaw plum (Prunus angustifolia Marsh.). We studied growth of this species in one county in north-central (Payne) and two counties in northwestern Oklahoma (Ellis, Harper) during 2006 and 2007. We estimated age of stems and roots by growth rings and area of stands with the use of a handheld GPS unit. Based on zero-intercept regression models, stands grew at similar rates (overlapping 95% confidence intervals [CIs]) among counties with a pooled estimate of 31.0 m² · yr⁻¹ (95% CI = 26.5–35.6 m² · yr⁻¹; n = 95). This rate showed considerable variability within and among study sites (r = 0.52). Stem diameter increased (zero-intercept models) more rapidly in north-central Oklahoma (5.27 mm · yr⁻¹; 95% CI = 5.01–5.53 mm · yr⁻¹; r = 0.90; n = 53) than in northwestern Oklahoma (3.68 mm · yr⁻¹; 95% CI = 3.55–3.81 mm · yr⁻¹; r = 0.91; n = 102); data were pooled because of similar rates in Ellis and Harper counties. Stem height was a power function of stem age (y = 0.97x^0.28; r = 0.56), indicating rate of growth in height (m · yr⁻¹) declined with age according to dy/dx = 0.27x^−0.72. Knowledge of the area expansion rate of Chickasaw plum clones aids in management planning to increase or decrease canopy coverage by this shrub.     

Method of Supplementation May Affect Cattle Grazing Patterns

Supplement placement can be used to manipulate livestock grazing patterns. The objective of this case study was to compare the effect of low-moisture blocks (LMB) and range cake (barley-based cylindrical cubes, 2 cm in diameter, and 2 to 8 cm long) supplementation on cattle grazing patterns in Montana foothill rangeland. One group of nonlactating cows (n = 79) was fed cake 3 times per week (1.8 kg · cow⁻¹ · feeding⁻¹), and the other group (n = 81) had continuous access to LMB in separate pastures using a crossover design. Movement patterns of cows were recorded with global positioning system collars during four periods (2 wk · period⁻¹) during autumn. Range cake was fed on accessible areas, and LMB were placed in higher and steeper terrain. Intake of LMB averaged (mean ± SE) 318 ± 50 g · d⁻¹. Cows fed LMB (8.07° ± 0.20°) were observed on steeper slopes (P = 0.08) than cows fed range cake (6.96° ± 0.19°). Forage utilization decreased as slope increased to a greater degree when range cake was fed than when LMB was fed (P = 0.001). Cows spent more time (P = 0.05) within 100 m of LMB (274 ± 23 min · d⁻¹) than at range cake feeding sites (67 ± 24 min · d⁻¹). Strategic placement of LMB on high, steep terrain appears to be a more practical and effective approach than traditional hand-feeding range cake on intermediate terrain to improve uniformity of cattle grazing on rugged rangeland.     

Infiltration,Runoff, and Sediment Yield in Response to Western Juniper Encroachment in Southeast Oregon

Infiltration was measured in a western juniper (Juniperus occidentalis Hook.) watershed to characterize the hydrologic processes associated with landscape position. Infiltration rate, runoff, and sediment content were measured with the use of a small-plot rainfall simulator. Study sites were located in each of the four primary aspects (north, south, east, and west). Research sites were located in two ecological sites—South Slopes 12–16 PZ and North Slopes 12–16 PZ. Within aspect, plots were located in three juniper cover levels: high (> 22%), moderate (13%–16%), and low (<3%) juniper canopy cover. During rainfall simulation, water was applied at a 10.2-cm · h⁻¹ rate, levels comparable to an infrequent, short-duration, high-intensity precipitation event. Runoff was measured at 5-min intervals for 60 min. Comparing canopy cover levels, steady-state infiltration rates on control plots (9.0 cm · h⁻¹) was 68% greater than high juniper cover sites (2.87 cm · h⁻¹) and 34% greater than moderate juniper cover sites (5.97 cm · h⁻¹) on south-facing slopes. On north-facing slopes, no differences in infiltration rates were observed between juniper cover levels, demonstrating differential hydrologic responses associated with ecological site. Generally, all water applied to control plots infiltrated. Highest infiltration rates were positively associated with increased surface litter and shrub cover. In addition, depth of water within the soil profile was lowest in high juniper cover plots. This suggests that less water is available to sustain understory and intercanopy plant growth in areas with high juniper cover. Accelerated runoff and erosion in juniper dominated sites (high level) across east-, west-, and south- facing slopes can lead to extensive degradation to the hydrology of those sites. These data suggest that sustained hydrologic processes are achieved with reduced western juniper canopy cover.     

Rough Agave Flowers as a Potential Feed Resource for Growing Goats

The objective of this study was to evaluate the effect of different levels of rough agave (Agave scabra Ortega) flowers on dry matter intake (DMI), average daily gain (ADG), volatile fatty acid (VFA) production in the rumen, and particular serum metabolites and minerals of native × dairy growing goats (Capra hircus L.). Forty female goats with an initial weight of 11.1 ±  kg (mean ± SD) were used in a completely randomized design experiment that lasted for 84 d. Goats were fed a completely mixed ration (30% roughage, 70% ground corn [Zea mays L.] and soybean [Glycine max {L.} Merr] meal). Treatments consisted of offering goats (4 pens · group⁻¹, 2 goats · pen⁻¹) air-dry rough agave flowers, which replaced alfalfa (Medicago sativa L.) hay at 0% (control; T0), 25% (T25), 50% (T50), 75% (T75), and 100% (T100) of the of the roughage portion of the diet. Values of nutritional parameters for rough agave flowers were in vitro organic matter digestibility, 493 g · kg⁻¹; crude protein, 115 g · kg⁻¹; and metabolizable energy, 6.29 MJ · kg⁻¹ DMI. There were differences (P < 0.05) in ADG (range, 108–155 g · d⁻¹) between diets. Goats fed T0 had higher (P < 0.05) gains than goats fed T50 and T100. DMI was not affected by dietary treatments (range, 3.4% to 3.6% of body weight). Feed conversion ratio (FCR, defined as DMI/ADG) increased (P < 0.05) 27% with total substitution of alfalfa by rough agave flowers, in comparison with T0. Lower (P < 0.05) values of total VFA were obtained with T100, in comparison with all other dietary treatments. These results demonstrated that totally replacing alfalfa with rough agave flowers in diets did not affect DMI but decreased AGD and compromised FCR. Thus, rough agave flowers have the potential to partially replace alfalfa in diets for growing goats.     

Net Carbon Fluxes Over Burned and Unburned Native Tallgrass Prairie

Prescribed burning of aboveground biomass in tallgrass prairie is common and may influence dynamics and magnitudes of carbon (C) movement between the surface and atmosphere. Carbon dioxide (CO₂) fluxes were measured for 2 yr using conditional sampling systems on two adjacent watersheds in an ungrazed tallgrass prairie near Manhattan, Kansas. One watershed was burned annually (BA) and the other biennially (BB). Leaf and soil CO₂ fluxes were measured in the source area. Net ecosystem exchange (NEE) of CO₂ reached a maximum daily gain of 26.4 g CO₂·m^?2·d^?1 (flux toward surface is positive) in July 1998 (year when both sites were burned and precipitation was above normal); gains were similar between sites in 1998. The maximum daily NEE loss of CO₂ was ?21.8 g CO₂·m^?2·d^?1 from BA in September 1997 (year when only BA was burned and precipitation was below normal). When data were integrated over the two years, both sites were net sources of atmospheric CO₂; NEE was ?389 g C·m^?2·2 yr^?1 on BA and ?195 g C·m^?2·2 yr^?1 on BB. Burning increased canopy size and photosynthesis, but the greater photosynthesis was offset by corresponding increases in respiration (from canopy and soil). Carbon losses from fire represented 6–10% of annual CO₂ emissions (bulk came from soil and canopy respiration). Data suggest that annual burning promotes C loss compared to less-frequently burned tallgrass prairie where prairie is not grazed by ungulates. Greater precipitation in 1998 caused large increases in biomass and a more positive growing season NEE, indicating that C sequestration appears more likely when precipitation is high. Because C inputs (photosynthesis) and losses (canopy and soil respiration) were large, small measurement or modeling errors could confound attempts to determine if the ecosystems are long-term CO₂ sources or sinks.     

The effect of maropitant on intraoperative isoflurane requirements and postoperative nausea and vomiting in dogs: a randomized clinical trial

ObjectiveTo establish if preoperative maropitant significantly reduced intraoperative isoflurane requirements and reduced clinical signs associated with postoperative nausea and vomiting (PONV) in dogs.Study designRandomized clinical trial.AnimalsTwenty-four healthy, client-owned dogs undergoing routine ovariohysterectomy.MethodsPremedication involved acepromazine (0.03 mg kg⁻¹) combined with methadone (0.3 mg kg⁻¹) intramuscularly 45 minutes before anaesthetic induction with intravenous (IV) propofol, dosed to effect. Meloxicam (0.2 mg kg⁻¹) was administered intravenously. Dogs were randomly assigned to administration of saline (group S; 0.1 mL kg⁻¹, n = 12) or maropitant (group M; 1 mg kg⁻¹, n = 12) subcutaneously at time of premedication. Methadone (0.1 mg kg⁻¹ IV) was repeated 4 hours later. Anaesthesia was maintained with isoflurane in oxygen, dosed to effect by an observer unaware of group allocation. The dogs were assessed hourly, starting 1 hour postoperatively, using the short form of the Glasgow Composite Pain Score (GCPS), and for ptyalism and signs attributable to PONV [score from 0 (none) to 3 (severe)] by blinded observers. Owners completed a questionnaire at the postoperative recheck.ResultsOverall mean ± standard deviation end-tidal isoflurane percentage was lower in group M (1.19 ± 0.26%) than group S (1.44 ± 0.23%) (p = 0.022), but was not significantly different between groups at specific noxious events (skin incision, ovarian pedicle clamp application, cervical clamp application, wound closure). Cardiorespiratory variables and postoperative GCPS were not significantly different between groups. Overall, 50% of dogs displayed signs attributable to PONV, with no difference in PONV scores between groups (p = 0.198). No difference in anaesthetic recovery was noted by owners between groups.ConclusionsMaropitant reduced overall intraoperative isoflurane requirements but did not affect the incidence of PONV.Clinical relevanceMaropitant provided no significant benefits to dogs undergoing ovariohysterectomy with this anaesthetic and analgesic protocol, although clinically significant reductions in isoflurane requirements were noted.     

Evaluation of Low-Stress Herding and Supplement Placement for Managing Cattle Grazing in Riparian and Upland Areas

Management practices are often needed to ensure that riparian areas are not heavily grazed by livestock. A study was conducted in Montana during midsummer to evaluate the efficacy of low-stress herding and supplement placement to manage cattle grazing in riparian areas. Three treatments were evaluated in three pastures over a 3-yr period in a Latin-square design (n = 9). Each year, naïve 2-yr-old cows with calves were randomly assigned to the three treatments: 1) free-roaming control, 2) herding from perennial streams to upland target areas, and 3) herding to upland sites with low-moisture block supplements. Stubble heights along the focal stream were higher (P = 0.07) in pastures when cattle were herded (mean ± SE, 23 ± 2 cm) than in controls (15 ± 3 cm). Global positioning system telemetry data showed that herding reduced the time cows spent near (< 100 m) perennial streams (P = 0.01) and increased the use of higher elevations (P = 0.07) compared with controls. Evening visual observations provided some evidence that free-roaming cows (44% ± 19%) were in riparian areas more frequently (P = 0.11) than herded cows (23% ± 6%). Fecal abundance along the focal stream was less (P = 0.07) with herding (61.9 ±  kg · ha⁻¹) than in controls (113.2 ±  kg · ha⁻¹). Forage utilization within 600 m of supplement sites was greater (P = 0.06) when cows were herded to low-moisture blocks (18% ± 6%) compared with controls and herding alone (8% ± 2%). Moving cattle to uplands at midday using low-stress herding is an effective tool to reduce use of riparian areas. Herding cattle to low-moisture blocks can increase grazing of nearby upland forage but may not provide additional reduction in cattle use of riparian areas compared with herding alone.     

Effects of Grazing Intensity,Precipitation, and Temperature on Forage Production

Questions have been raised about whether herbaceous productivity declines linearly with grazing or whether low levels of grazing can increase productivity. This paper reports the response of forage production to cattle grazing on prairie dominated by Kentucky bluegrass (Poa pratensis L.) in south-central North Dakota through the growing season at 5 grazing intensities: no grazing, light grazing (1.3 ±  animal unit months [AUM] · ha^-1), moderate grazing (2.7 ±  AUM · ha^-1), heavy grazing (4.4 ±  AUM · ha^-1), and extreme grazing (6.9 ±  AUM · ha^-1; mean ± SD). Annual herbage production data were collected on silty and overflow range sites from 1989 to 2005. Precipitation and sod temperature were used as covariates in the analysis. On silty range sites, the light treatment produced the most herbage (3 410 kg · ha^-1), and production was reduced as the grazing intensity increased. Average total production for the season was 545 kg · ha^-1 less on the ungrazed treatment and 909 kg · ha^-1 less on the extreme treatment than on the light treatment. On overflow range sites, there were no significant differences between the light (4 131 kg · ha^-1), moderate (4 360 kg · ha^-1), and heavy treatments (4 362 kg · ha^-1; P &spigt; 0.05). Total production on overflow range sites interacted with precipitation, and production on the grazed treatments was greater than on the ungrazed treatment when precipitation (from the end of the growing season in the previous year to the end of the grazing season in the current year) was greater than 267.0, 248.4, 262.4, or 531.5 mm on the light, moderate, heavy, and extreme treatments, respectively. However, production on the extreme treatment was less than on the ungrazed treatment if precipitation was less than 315.2 mm. We conclude that low to moderate levels of grazing can increase production over no grazing, but that the level of grazing that maximizes production depends upon the growing conditions of the current year.     

Grazing Systems for Yearling Cattle on Tallgrass Prairie

This 9-yr study tested steer gains, residual aboveground biomass (AGB) in mid-July and early October, and economic returns and risk for tallgrass prairie grazed annually under season-long stocking (SLS) at 1.62 ha · steer⁻¹ until early October or intensive early stocking (IES) at 0.81 ha · steer⁻¹ until mid-July compared to a composite grazing system. The three-pasture, three-herd “IES+ System” is a 3-yr fixed sequence of SLS, IES, and IES (0.81 ha · steer⁻¹) plus late-season grazing (LSG; 1.62 ha · steer⁻¹) until early October (IES/LSG). All grazing treatments began in late April. Average gains per steer for SLS and SLS in the IES+ System did not differ, but were significantly less than gains for steers that grazed the entire season under IES/LSG. Gains per steer in mid-July under IES alone or in combination with LSG were similar to the same repeated grazing treatments, but were significantly less than those for steers grazed season-long. Gains per hectare under SLS did not differ, but were significantly less than those for IES treatments and the IES+ System. Gain per hectare in July was similar for IES repeated annually and IES/LSG, but there was greater gain per hectare for IES-treated pastures rotated within the system. Residual grass and total aboveground biomass (AGB) in mid-July did not vary among years and was generally greater on SLS than IES. In early October, grass AGB was similar for all treatments except IES/LSG, which had less residual AGB. When pasture rent was charged per head, the IES+ System increased the 20-yr mean return per hectare by $5.98 compared to repeated use of IES, and $8.52 compared to using only SLS. Measures of economic risk were generally intermediate for the IES+ system compared to IES, which consistently had the highest risk, and SLS.     

Leafy Spurge Suppression by Flea Beetles in The Little Missouri Drainage Basin,USA

The Ecological Area-wide Management Leafy Spurge, or TEAM Leafy Spurge, began collecting and redistributing flea beetles (Aphthona spp.) to research/demonstration sites and landowners throughout the Little Missouri River drainage basin to control leafy spurge in 1998. A study to evaluate the change over time of leafy spurge (Euphorbia esula L.) phytosociological characteristics following release of flea beetles was initiated in 2002 on leafy spurge–infested pasture and rangeland in the Little Missouri River drainage of Montana, North Dakota, South Dakota, and Wyoming. A total of 292 flea beetle release sites were analyzed in June and July 2002 and 2003 for leafy spurge stem density, foliar cover, flea beetle density, and vegetation composition. Leafy spurge stem density suppression was evident at 91% of the study sites. On two-thirds of the study sites stem density was reduced from greater than 100 stems·m⁻¹ to less than 25 stems·m⁻¹. Leafy spurge foliar cover was less than 5% on approximately two-thirds of the flea beetle release sites and less than 25% on over 90% of the release sites. Area of observed leafy spurge suppression ranged from 0 m² to 30000 m². Approximately 40% of the release sites had leafy spurge suppression ranging from 1000 m² to 5000 m², and 14% of the release sites had greater than 10000 m² of leafy spurge control. Plant community composition following leafy spurge suppression was characteristic of native plant communities that had not been burned or grazed. Flea beetles effectively reduced leafy spurge stem density and cover in 4–5 yr across a variety of locations and corresponding environmental conditions, both within the Little Missouri River drainage and in selected nearby locations.     

The Effect of Dairy Compost on Summer Annual Dicots Grown as Alternative Silages

Kenaf (Hibiscus cannibinus) var. “India”, lablab (Lablab purpureus) variety “Tecomate,” combine and iron-clay cowpeas (Vigna unguiculata), and sunflowers (Helianthus annuus) S682 and S573 were planted at the Stephenville Research and Extension Center in the spring seasons of 1998 and 1999. Dairy compost was incorporated into half the plots prior to seeding both years at 11.2 t DM ha⁻¹. Average days to maturity and number of harvests per season varied from 66 to 84 d and 1 to 3 harvests, respectively. Average yields were highest for kenaf (13,762 kg DM ha⁻¹ yr⁻¹) followed by lablab (7,925 kg DM ha⁻¹ yr⁻¹). Acid detergent fiber and lignin DM concentrations were lowest in kenaf (average 26.9 and 3.57%, respectively) and cowpeas (average 25.9 and 4.39%, respectively). Crude protein and phosphorus (P) concentrations were high in the iron-clay cowpea (22.3 and 0.269, respectively), combine cowpea (18.3 and 0.276%, respectively), lablab (16.8 and 0.268%, respectively), and kenaf (14.9 and 0.204%, respectively) and relatively low in the sunflower (9.7 and 0.174%, respectively). Compost increased forage yields 21.6% and forage P concentration 18.8% following the second year’s application. Compost was applied to supply 130 kg P ha⁻¹ yr⁻¹; on average, forages removed only 34.2 kg P ha⁻¹ yr⁻¹. As a result, soil P was 2.4 times higher in plots with compost (26.9 ppm) by the end of the 2-yr trial. Kenaf had the highest P uptake, averaging 56.6 kg P ha⁻¹ over 2 yr, accounting for 43% of the P applied.     

Crested Wheatgrass Control and Native Plant Establishment in Utah

Effective control methods need to be developed to reduce crested wheatgrass (Agropyron cristatum [L.] Gaertner) monocultures and promote the establishment of native species. This research was designed to determine effective ways to reduce crested wheatgrass and establish native species while minimizing weed invasion. We mechanically (single- or double-pass disking) and chemically (1.1 L · ha^?1 or 3.2 L · ha^?1 glyphosate–Roundup Original Max) treated two crested wheatgrass sites in northern Utah followed by seeding native species in 2005 and 2006. The study was conducted at each site as a randomized block split plot design with five blocks. Following wheatgrass-reduction treatments, plots were divided into 0.2-ha subplots that were either unseeded or seeded with native plant species using a Truax Rough Rider rangeland drill. Double-pass disking in 2005 best initially controlled wheatgrass and decreased cover from 14% to 6% at Lookout Pass and from 14% to 4% at Skull Valley in 2006. However, crested wheatgrass recovered to similar cover percentages as untreated plots 2–3 yr after wheatgrass-reduction treatments. At the Skull Valley site, cheatgrass cover decreased by 14% on herbicide-treated plots compared to an increase of 33% on mechanical-treated plots. Cheatgrass cover was also similar on undisturbed and treated plots 2 yr and 3 yr after wheatgrass-reduction treatments, indicating that wheatgrass recovery minimized any increases in weed dominance as a result of disturbance. Native grasses had high emergence after seeding, but lack of survival was associated with short periods of soil moisture availability in spring 2007. Effective wheatgrass control may require secondary treatments to reduce the seed bank and open stands to dominance by seeded native species. Manipulation of crested wheatgrass stands to restore native species carries the risk of weed invasion if secondary treatments effectively control the wheatgrass and native species have limited survival due to drought.     

Fire and Nitrogen Effects on Purple Threeawn (Aristida purpurea) Abundance in Northern Mixed-Grass Prairie Old Fields

Purple threeawn (Aristida purpurea Nutt. varieties) is a native grass capable of increasing on rangelands, forming near monocultures, and creating a stable state. Productive rangelands throughout the Great Plains and Intermountain West have experienced increases in purple threeawn abundance, reducing overall forage quality. Our objectives were to 1) reveal the effects of prescribed fire and nitrogen amendments on purple threeawn abundance and 2) assess nontarget plant response posttreatment. Season of fire (no fire, summer fire, fall fire) and nitrogen addition (0 kg N · ha^?1, 46 kg N · ha^?1, and 80 kg N · ha^?1) were factorially arranged in a completely randomized design and applied to two similar sites in southeastern Montana. We evaluated fire and nitrogen effects on purple threeawn basal cover, relative composition, and current-year biomass one growing season postfire at two sites treated during different years. Spring weather following fire treatments was very different between years and subsequently impacted community response. Initial purple threeawn biomass at both sites was 1 214 ± 46 kg · ha^?1 SEc. When postfire growing conditions were wet, current-year biomass of purple threeawn was reduced 90% and 73% with summer and fall fire, respectively. Under dry postfire growing conditions, purple threeawn current-year biomass was reduced 73% and 58% with summer and fall fire, respectively. Nitrogen additions had no effect on purple threeawn current-year biomass at either site. Current-year biomass of C₃ perennial grass doubled with nitrogen additions and was not impacted by fire during a wet spring. Nitrogen additions and fire had no effect on C₃ perennial grass current-year biomass following a dry spring. Prescribed fire appears to be a highly effective tool for reducing purple threeawn abundance on semiarid rangelands, with limited detrimental impacts to nontarget species.     

Pharmacokinetic,metabolism and withdrawal time of orphenadrine in camels (Camelus dromedarius) after intravenous administration

The pharmacokinetics of orphenadrine (ORPH) following a single intravenous (i.v.) dose was investigated in six camels (Camelus dormedarius). Orphenadrine was extracted from the plasma using a simple sensitive liquid–liquid extraction method and determined by gas chromatography/mass spectrometry (GC/MS). Following i.v. administration plasma concentrations of ORPH decline bi-exponentially with distribution half-life (t_1/2α) of 0.50 ± 0.07 h, elimination half-life (t_1/2β) of 3.57 ± 0.55 h, area under the time concentration curve (AUC) of 1.03 ± 0.10 g/h l⁻¹. The volume of distribution at steady state (Vd_ss) 1.92 ± 0.22 l kg⁻¹, volume of the central compartment of the two compartment pharmacokinetic model (V_c) 0.87 ± 0.09 l kg⁻¹, and total body clearance (Cl_T) of 0.60 ± 0.09 l/h kg⁻¹. Three orphenadrine metabolites were identified in urine samples of camels. The first metabolite N-desmethyl-orphenadrine resulted from N-dealkylation of ORPH with molecular ion m/z 255. The second N,N-didesmethyl-orphenadrine, resulted from N-didesmethylation with molecular ion m/z 241. The third metabolite, hydroxyl-orphenadrine, resulted from the hydroxylation of ORPH with molecular ion m/z 285. ORPH and its metabolites in camel were extensively eliminated in conjugated form. ORPH remains detectable in camel urine for three days after i.v. administration of a single dose of 350 mg orphenadrine aspartate.     

α-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standardbred trotters

Heat shock protein (HSP) expression is an adaptive mechanism against the disruption of cell homeostasis during exercise. Several antioxidant supplementation strategies have been used to enhance tissue protection. In this study, we examined the effects of a redox modulator, α-lipoic acid (LA) on HSP responses in six standardbred trotters following intense aerobic exercise. DL–LA supplementation (25 mg kg⁻¹ d⁻¹) for five weeks increased the resting levels of HSP90 (1.02 ± 0.155 in control and 1.26 ± 0.090 after supplementation in arbitrary units) and the recovery levels of inducible HSP70 (0.89 ± 0.056 in control and 1.05 ± 0.089 after supplementation in arbitrary units) in skeletal muscle. Furthermore, LA increased skeletal muscle citrate synthase activity at rest and lowered the blood lactate concentration during exercise without any changes in the heart rate. LA had no effect on concentrations of HSP60, HSP25 or GRP75 in skeletal muscle. LA decreased the exercise-induced increases in plasma aspartate aminotransferase and creatine kinase concentrations during recovery. Our results suggest that LA supplementation may enhance tissue protection and increase oxidative capacity of the muscle in horse.     

Pharmacokinetics of mequindox and its metabolites in rats after intravenous and oral administration

Pharmacokinetics of mequindox (MEQ) and its metabolites were determined in rats after intravenous (i.v.) and oral (p.o.) administration of MEQ at a single dose of 10 mg kg⁻¹ bodyweight. After both administrations, MEQ and five of its metabolites were quantified, except M4, whereas M1 and M2 were the predominant ones. The areas under the concentration–time curves (h ng mL⁻¹) of MEQ, M1, M2, M3, M5 and M10 after i.v. administration were 7559 ± 495, 6354 ± 2761, 5586 ± 2337, 1034 ± 160, 2370 ± 791 and 1813 ± 622, respectively, whereas after p.o. administration, remained as 2809 ± 40, 4361 ± 3544, 4351 ± 1046, 1444 ± 814, 3864 ± 305 and 1213 ± 569, respectively. The elimination half-lives (h) of these compounds after i.v. administration were 3.48 ± 0.80, 4.20 ± 0.76, 6.25 ± 2.41, 4.77 ± 1.54, 4.69 ± 1.62 and 16.89 ± 5.15, respectively, and were 3.21 ± 0.40, 3.66 ± 1.06, 4.20 ± 1.03, 8.91 ± 5.99, 4.20 ± 2.02 and 20.84 ± 10.85 after p.o. administration, respectively. After p.o. administration, the bioavailability of MEQ was 37.16%. The results showed that MEQ was extensively metabolized in rats and rapidly absorbed after p.o. administration.     

Animal and Vegetation Response to Modified Intensive–Early Stocking on Shortgrass Rangeland

A comparison of animal gains and vegetation trends was made from 2002–2008 between a continuous season-long stocking (SLS) system and a modified intensive–early stocking system (IES) with late-season grazing (IES 1.6× + 1; 1.6 times the number of animals of the SLS system from May 1 to July 15, and 1 times the number of animals of SLS from July 15 to October 1) on shortgrass native rangeland of western Kansas. The continuous season-long stocked system placed animals at a density of 1.37 ha · steer^?1 from May through October, or 2.63 animal unit months (AUM) · ha^?1, whereas the intensive–early stocked system with late-season grazing (3.33 AUM · ha^?1) stocked pastures at 0.85 ha · steer^?1 from May through the middle of July, and then stocked pastures at 1.37 ha · steer^?1 for the remainder of the grazing season by removing the heaviest animals mid-July each yr. Average daily gains (0.78 vs. 0.70 kg · d^?1, P = 0.039) and total animal gain (58 vs. 52 kg, P = 0.042) were different between the continuous season-long stocked and the intensive–early stocked animals during the first half of the grazing season. No difference was found between average daily gain (0.61 vs. 0.62 kg · d^?1, P = 0.726) and total animal gain (48 vs. 49 kg, P = 0.711) for the continuous season-long stocked and intensive–early stocked with late-season grazing animals during the last half of the season. Total individual animal gain (106 vs. 101 kg, P = 0.154) and average daily gain (0.70 vs. 0.66 kg · d^?1, P = 0.152) was not different between the continuous season-long stocked and the intensive–early stocked system animals that were on pasture the entire grazing season. Total beef gain on a land-area basis (96 vs. 77 kg · ha^?1, P = 0.008) was greater for the modified intensive–early stocked system with late-season grazing with greater animal densities. Changes in residual biomass and most key vegetation components at the end of the grazing season were not different between the two systems.