Effects of suni-bug (Eurygaster spp.) damage on semolina properties and spaghetti quality characteristics of durum wheats (Triticum durum L.)

Effects of suni-bug (Eurygaster spp.) damage on semolina properties and spaghetti quality characteristics of durum wheats (Triticum durum L.) were investigated. The semolinas obtained from sound (control), medium damage (around 20%) and high damage (around 40%) samples of five durum wheat cultivars (cvs. Diyarbakir, Firat, Ege, Svevo and Zenith) were processed into spaghetti. As the bug damage level increased, Glutograph stretch values of all cultivars decreased significantly probably due to deteriorative effects of bug damage on gluten quality. Glutograph relaxation values and gluten spread values of the damaged samples were considerably higher compared to those of sound samples in all cultivars, due to proteolytic degradation. The breaking force of the uncooked spaghetti samples decreased significantly with increasing bug damage level indicating that they were susceptible to breakage and not suitable for handling, packaging and shipment. Panel tests indicated significant deterioration in sensory properties (stickiness, firmness and bulkiness) generally at the medium damage level.     

Determination of wheat bug (Eurygaster spp.) damage in durum wheat (Triticum durum L.) by electrophoresis and rapid visco analyser

The degradation effects of wheat bug protease(s) on glutenin proteins of durum wheat cultivars were investigated by electrophoresis and modified rapid visco analyser (RVA) test. Glutenin patterns of the bug damaged durum wheats changed substantially due to bug protease(s). Although high molecular weight glutenin subunits (HMW-GS) of three cultivars (Ege, Svevo, and Zenith) disappeared after 60 min of incubation, the HMW-GS of other two cultivars (Diyarbakir and Firat) were still visible even after the longest incubation period at medium damage level. It shows that there was an intercultivar variation in susceptibility to hydrolysis by bug proteolytic enzymes. Low molecular weight glutenin subunits of all cultivars decreased substantially after 30 min of incubation. The RVA curves indicated a clear reduction in viscosity in semolina samples with both medium and high damage levels as compared to their respective undamaged (control) samples. There were significant correlations (p < 0.001) between bug damage level and viscosities at 3 min (r = −0.765), at 4.5 min (r = −0.549) and at 10 min (r = −0.835), breakdown value (r = −0.534) and decay rate (r = 0.600). Consequently, hydrolysis rate of wheat bug protease(s) can be determined by modified RVA technique without much more chemicals, procedures and expensive equipments.     

六个强筋小麦品种不同出粉点面粉和面片的色泽及其褐变特点

为研究不同出粉点面粉面条色泽品质的差异,测定了6个强筋小麦品种通过布勒实验磨制粉获得的不同出粉点面粉的品质特性与面片色泽及褐变情况。结果表明,3道心磨和3道皮磨的面粉和面片色泽的L^*值均随出粉点后移呈下降趋势,而a^*值和b^*值则呈上升趋势;在面片放置过程中,3道心磨和3道皮磨面粉所加工面片色泽亮度的下降值、偏红度的上升值和偏黄度的上升值均随出粉点后移呈加速趋势。面片色泽与蛋白质含量、灰分含量和面粉L^*值关系密切。鲜面片放置24 h后色泽亮度的下降值与面粉粗蛋白质含量和灰分含量均呈极显著正相关,相关系数分别为0.444和0.758,而与面粉L^*值呈极显著负相关,相关系数为-0.681;色泽偏红度的上升值与面粉灰分和破损淀粉含量均呈极显著正相关,相关系数分别为0.834和0.760;色泽偏黄度的上升值与所有指标的相关均不显著。3心粉的色泽差,其所加工的面片色泽也较差,且色泽不稳定,在面片放置过程中色泽的亮度、偏红度和偏黄度保持性最差,褐变程度严重;1心粉的色泽好,其所加工的面色泽好,并在面片放置过程中,色泽的亮度和偏红度保持性较好,而色泽的偏黄度不如1皮粉和2皮粉的稳定。     

Assessment of antioxidant activity and rheological properties of wheat and buckwheat milling fractions

This study examined the antioxidant properties of the ethanolic extracts of wheat milling fractions (wheat flour type 500 and type 850, and bran) and their polyphenol and tocopherol content, and rheological characteristics of wheat dough supplemented with buckwheat flours (light and wholegrain). The results obtained in this study were correlated with our previously published data on wheat flour type 400, wholegrain wheat flour and buckwheat flours.Buckwheat flours exhibited significantly higher (P < 0.05) antiradical activity on hydroxyl (OH), superoxide anion (O₂⁻) and (1,1-diphenyl-2-picrylhydrazyl) DPPH radicals, antioxidant activity and reducing power than all investigated wheat milling fractions when their corresponding IC₅₀ values were compared.The rheological parameters of wheat dough supplemented with light and wholegrain buckwheat flour (0-50%) were obtained by using Mixolab. Results indicated changes in protein and starch properties of dough.The obtained results indicate the benefit of using buckwheat flours in wheat-based food products, i.e. their contribution in functional and tailor-made-food production.     

Water-extractable Arabinoxylan from Pearled Flours of Wheat,Barley, Rye and Triticale. Evidence for the Presence of Ferulic Acid Dimers and their Involvement in Gel Formation

Cereal grains from wheat, barley, triticale and rye were pearled and milled. Part of the flours were treated in boiling aqueous ethanol and both the ethanol-treated and untreated flours were extracted with water. Yields of arabinoxylan in the extracts were higher for the untreated flours. Chemical, physico-chemical and structural studies showed differences between the arabinoxylans in extracts of the different cereals although arabinoxylans extracted from untreated and the corresponding ethanol treated flours were similar in neutral sugar content and patterns of substitution. Analyses showed that low amounts of ferulic acid dimers (8-O-4′, 8-5′, and 5-5′ forms) were present in all the extracted arabinoxylans. Oxidative gelation studies, using peroxidase/H₂O₂, revealed significant differences in the capacity of extracted arabinoxylans to form gels via intermolecular cross-linking. The values of elastic modulus (G′) of gels from the arabinoxylan extract of ethanol-treated flours varied from 2 to 40 Pa and were higher than those obtained from the untreated flours (0·2–6 Pa).     

The influence of milling on the nutritive value of flour from cereal grains. 2. Wheat

Wheat was milled into flours having extraction rates between 100 and 66%. The nutritive value of the various fractions was studied by chemical analyses and in balance trials with rats. The concentration of essential nutrients decreased when the extraction rate was lowered. The lysine content (g/16 g N) e.g. was 2.52 in whole wheat, but only 2.18 in the 66% extraction flour; however, only a slight reduction in biological value was found. The content of minerals was reduced to 30% of that in whole wheat, and the apparent zinc absorption and retention expressed in absolute values, were significantly higher from the flours of high extraction than from the more refined flours, in spite of a much higher phytate content in whole wheat and lightly milled flours. It could be concluded that milling of wheat into highly refined flours not only preclude considerable amounts of nutrients from human consumption, but the remaining flours have a much poorer nutritive value than flour made from whole wheat.     

不同小麦粉混配品质性状的变化

为了研究小麦粉混配过程中品质性状的变化，分析了陕西省13个推广小麦品种及其23种混配粉的蛋白质品质和面团流变学特性。结果表明，强筋小麦不同批次制粉品质变化较大，中筋和弱筋小麦品质变化相对较小，不同方式配粉面团流变学特性变化规律不同。强筋 中筋和强筋 弱筋两种方式配粉稳定时间测定值比理论值减少，延伸性有增有减。同时，强筋 中筋=强筋的配粉延伸性以减小为主，而强筋 中筋=中筋的配粉延伸性增加，2个中筋粉或中筋与弱筋粉混配延伸性明显增加。混配粉面筋含量、沉淀值、稳定时间和延伸性测定值与由基础品种估算的理论值无显著差异，而且呈极显著正相关，相关系数分别为0．9818^**、0．9776^**、0．8594^**和0．7189^**。文中还提出了根据配粉需要估算基础品种混配比例的方法。     

Texturized Pinto Bean Protein Fortification in Straight Dough Bread Formulation

Pinto beans were milled and then air-classified to obtain a raw high protein fraction (RHPF) followed by extrusion to texturize the protein fraction. The texturized high protein fraction (THPF) was then milled to obtain flour, and combined with wheat flour at 5, 10, and 15 % levels to make bread. The air-classification process produced flour with high concentration of lipids and phytic acid in the protein-rich fraction. However, extrusion significantly reduced hexane extractable lipid and phytic acid. However, the reduction observed may simply indicate a reduction in recovery due to bind with other components. Total protein and lysine contents in composite flours increased significantly as THPF levels increased in composite flour. Bread made with 5 % THPF had 48 % more lysine than the 100 % wheat flour (control). The THPF helped to maintain dough strength by reducing mixing tolerance index (MTI), maintaining dough stability and increasing departure time on Farinograph. Bread loaf volume was significantly reduced above 5 % THPF addition. THPF increased water absorption causing an increase in bread weights by up to 6 %. Overall, loaf quality deteriorated at 10 and 15 % THPF levels while bread with 5 % THPF was not significantly different from the control. These results support the addition of 5 % THPF as a means to enhance lysine content of white pan bread.     

Improved functional properties for soy–wheat doughs due to modification of the size distribution of polymeric proteins

Physical modification of soy flour was shown to greatly improve the dough and baking qualities of soy–wheat (1:1) composite doughs, compared to raw soy flour, giving better stability and R_max, although extensibility was still below that of the wheat dough.Reasons for improvements caused by the physical-modification process were sought by determining the relative size distribution of proteins in the soy–wheat composite doughs by size-exclusion high-performance liquid chromatography (SE-HPLC). Results were expressed as the proportion of ‘unextractable polymeric protein’ (%UPP)—the proportion of the protein that is over 100,000 Da and only extractable after sonication. Protein extracts from the soy–wheat dough were sampled at different stages of dough mixing and fermentation, and their molecular-size distributions evaluated.Unextractable soy proteins were lower in raw soy flour (only 8% UPP) than in two physically-modified soy flours (19 and 34% UPP, respectively). Unextractable polymeric protein was much greater for wheat flour (57% UPP). After mixing a 1:1 soy–wheat composite dough, the %UPP was 36 and 22 (for the two types) when made from physically modified soy flours, compared to 8 for a composite dough using raw soy flour, and 43 for a wheat-only dough. The higher proportion of UPP for the wheat-modified soy doughs was taken as a reason for this composite dough providing better dough and baking qualities. Prolonged fermentation time caused a decrease in UPP percentages for all composite doughs and for the wheat-only dough.     

Effect of Barley and Barley Components on Rheological Properties of Wheat Dough

The effects of addition of whole barley and barley components (starch, β-glucans and arabinoxylans) on rheological properties of dough prepared from wheat flours with variable gluten quality (cv. Glenlea, extra-strong; cv. Katepwa, very strong; cv. AC Karma, strong; and cv. AC Reed, weak) were investigated in these studies using Mixograph and dynamic rheological measurements. Whole barley meal, starch and non-starch polysaccharides from hulless barley with variable starch characteristics (normal, high amylose, waxy, and zero amylose waxy) were tested. Upon addition of either β-glucans or arabinoxylans, significant increases in peak dough resistance, mixing stability, and work input were recorded in all flours. The addition of starch to various wheat flours reduced the strength of the respective flour-water doughs. The improvement of dough strength upon addition of waxy or zero amylose waxy barley meal was associated with the high content of total and soluble β-glucans present in barley samples. The addition of arabinoxylans or β-glucans increased the G′ of wheat doughs; arabinoxylans had a greater effect than β-glucans. Starch substantially decreased the elastic modulus of dough prepared from cv. Glenlea but waxy and high amylose starches increased the G′ of dough prepared from cv. AC Karma. A combination of the high amounts of non-starch polysaccharides and unusual starch characteristics in barley seems to balance the negative effects associated with gluten dilution brought about by addition of barley into wheat flour.     

Relative contribution of wheat flour constituents to Solvent Retention Capacity profiles of European wheats

Solvent Retention Capacity (SRC) tests have originally been designed to predict flour functionality of North American wheat flours. A SRC profile consists of its Water Retention Capacity (WRC), Sodium Carbonate SRC (SCSRC), Sucrose SRC (SuSRC) and Lactic Acid SRC (LASRC) values. As the value of such tests when considering European wheat flours is rather unclear, we studied the chemical composition and SRC profiles of nineteen commercial European wheat flours. Their WRC, SCSRC, SuSRC and LASRC ranged from 56 to 66%, 74 to 88%, 90 to 102% and 106 to 147%, respectively. Both WRC and SCSRC were mostly higher than those of North American soft wheat flours, but in the range of those of North American hard wheat flours. For the entire set of European flours, strong linear relations were observed between the flour damaged starch levels and WRC values, as well as with SCSRC values. Modifying specific flour constituent properties and levels illustrated the contribution of proteins, damaged starch and arabinoxylan (AX) to the SRC values. Flour proteins, especially glutenins, mainly contributed to LASRC, while damaged starch largely impacted flour SCSRC. Water-extractable AX only contributed to the SuSRC values, whereas the solvent-accessible AX generally contributed to all SRC values, and particularly to the SuSRC values.     

Feature of air classification product in wheat milling: Physicochemical,rheological properties of filter flour

Wheat filter flours are by-products obtained from air-classification of wheat flour. Physicochemical and rheological properties of wheat filter flours were investigated in the present study. Average values of crude protein, gluten, lipid and damaged starch content of filter flours were higher than those of standard flours for the same batch. The positive correlation of particles with size <20 μm and damaged starch was found. Moreover, the filter flours had higher water absorption, stability time except head milling filter flour samples. Short peak time and low peak viscosity were also observed. Different composition of wheat filter flours may be an important factor influencing its properties. This study is very useful for exploring the utilization of wheat filter flours in the food industry.     

Effect of the addition of extruded wheat flours on dough rheology and bread quality

Extruded wheat flours, due to their increased water absorption capacity, constitute an opportunity to increase bread output in bakery production. However extrusion may modify dough and bread characteristics. The aim of this study was to investigate the effect of the substitution of 5% of the wheat flour by extruded wheat flour (produced with different time-temperature extrusion treatments) on dough mixing, handling and fermentation behaviour and bread volume, shape, texture and colour. The RVA curves indicate that extrusion intensity increases with increasing temperature or water content. Water absorption capacity rises with increasing treatment intensity, but dough stability tends to decrease. Adding extruded flours decreases dough extensibility but increases tenacity and gas production. Differences in dough structure were observed on photomicrography, though there were no clear differences in bread quality. These results indicate that it is possible to obtain adequate dough and bread characteristics using dough with 5% extruded wheat flour.     

Storage changes in the quality of sound and sprouted flour

Sound and sprouted flours (24 and 48 hr) from bread wheat (WL-1562), durum wheat (PBW-34) and triticale (TL-1210) were stored at room temperature (34.8°C) and relative humidity (66.7%) for 0, 45, 90 and 135 days to assess the changes in physico-chemical and baking properties. Protein, gluten, sedimentation value, starch and crude fat decreased during storage in all the samples; however, the decrease was more in sprouted flours. Free amino acids, proteolytic activity, diastatic activity and damaged starch decreased with increase in storage period. Total sugars and free fatty acids increased more rapidly in the flours of sprouted wheats during 135 days of storage. Loaf volume of breads decreased during storage in both sound and sprouted flour but the mean percent decrease in loaf volume was more in stored sound flours. Aging of sprouted flour for 45 days improved the cookie and cake making properties but further storage was of no value for these baked products.Chapati making properties of stored sound and sprouted flour were inferior to that of fresh counterparts.     

Modification of protein structure and dough rheological properties of wheat flour through superheated steam treatment

Native (NF, 13.5% w.b) and moistened (MF, 27% w.b) wheat flours were treated with superheated steam (SS) at 170 °C for 1, 2 and 4 min, and their protein structure as well as dough rheological properties were analyzed. Confocal laser scanning microscopy (CLSM) and SDS-PAGE patterns indicated the formation of protein aggregates with reduced SDS extractability after treatment. Farinograph and dynamic rheometry measurements showed that the strength as well as elastic and viscous moduli of the dough made from SS-treated flours progressively increased with SS treatment time. And both the improvements were more pronounced for superheated steam-treated moistened flours (SS-MF) than for superheated steam-treated native flours (SS-NF). Size-exclusion high performance liquid chromatography (SE-HPLC) analysis demonstrated that dough rheological parameters have positive correlations with SDS unextractable polymeric proteins (UPP) contents. SS treatment on flours led to a transition of protein secondary structures to more ordered form (α-helix and β-sheet). Additionally, free sulfhydryl (SH) contents decreased after treatment, which implied that disulfide bonds accounted for protein extractability loss and dough rheological properties improvement. Elevated moisture level promoted the modification of both protein structure and dough behaviors of flours during SS treatment.     

Study of the mechanism of improvement due to waxy wheat flour addition on the quality of frozen dough bread

Waxy wheat flour (WWF) was substituted for 10% regular wheat flour (RWF) in frozen doughs and the physicochemical properties of starch and protein isolated from the frozen doughs stored for different time intervals (0, 1, 2, 4 and 8 weeks) were determined to establish the underlying reasons leading to the effects observed in WWF addition on frozen dough quality. Using Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimeter (DSC) and X-ray Diffraction (XRD) among others, the gluten content, water molecular state, glutenin macropolymer content, damaged starch content, starch swelling power, gelatinization properties, starch crystallinity and bread specific volume were measured. Compared to RWF dough at the same frozen storage condition, 10% WWF addition decreased dry gluten and glutenin macropolymer contents and T₂₃ proton density of frozen dough, but increased the wet gluten content, T₂₁ and T₂₂ proton density. 10% WWF addition also decreased damaged starch content, but increased starch swelling power, gelatinization temperature and enthalpy, crystallinity of starch and bread specific volume of frozen dough. Results in the present study showed that the improvement observed due to WWF addition in frozen dough bread quality might be attributed to its inhibition of redistribution of water molecules bound to proteins, increase in damaged starch content and decrease in starch swelling power.     

Mixing behaviour of a zero-developed dough compared to a flour–water mixture

The Z-blade mixing behaviour of zero-developed (ZD) doughs from the flours of two wheat cultivars of different gluten strength was compared to that of conventionally mixed dough made from the same flours. In farinograph experiments, use of ZD dough led to shorter development time (with less energy requirement), less stability time, and consequently earlier breakdown compared to conventional mixing of the corresponding flour–water mixture. Mixing of ZD doughs led to an almost similar decrease of glutenin macro-polymer (GMP) wet weight as that of doughs prepared from flour–water mixtures. However, comparison of wet weight of re-assembled GMP revealed that until time-to-peak (TTP) mixing, there was no difference in GMP recovery with respect to the starting material used in the z-blade mixing experiments. Beyond TTP, recovery of GMP in doughs prepared from both starting materials was reduced. The results of large-strain deformation rheology showed strong visco-elastic behaviour as characterised by the highest values of fracture properties (except ε_H), followed by a decline in those properties upon further mixing for doughs mixed from both flour–water mixture and ZD dough from both types of wheat cultivars. It was concluded that at mixing regimes before TTP, there was no difference between ZD doughs and flour–water mixtures in the mixer. When ZD dough is used as a starting material for dough preparation instead of flour, extra care should be taken not to over-mix the developing dough.     

Wheat flour non-starch polysaccharides and their effect on dough rheological properties

Wheat (Triticum aestivum L.) flour is able to form dough with unique rheological properties that allow bread making. It is well known that wheat protein content affects dough rheological properties, but there is not enough evidence about the role of other minor flour constituents. One such minor constituent is non-starch flour polysaccharides, which are mainly pentosans formed by a xylopyranosyl linear chain branched with arabinofuranosyl residues. Their spatial distribution and branching pattern can affect their relationship with gluten forming proteins and thus influence their functional properties, the dough rheological properties, and thereby the flour baking quality. In this study the content and structural characteristics of non-starch polysaccharides were investigated, as well as their influence on some dough physicochemical parameters. Five different wheat flours samples milled from Uruguayan wheat varieties with diverse rheological and breadmaking properties were used in this study. Water soluble flour polysaccharides were extracted and the amount of pentosans was determined by the orcinol-HCl method. The pentoses composition was determined before and after acidic hydrolysis of the water soluble polysaccharide fractions by GC. No free pentoses were detected in any of the assayed flour samples, so the pentoses composition found in the hydrolyzed samples was attributed to the non-starch water soluble polysaccharides. Water unextractable non-starch polysaccharides were determined by difference between the total and the soluble non-starch polysaccharides flour content.An improved method for the quantification of water extractable and non-extractable non-starch polysaccharides, using baker's yeast, was developed. Using this method, total and soluble non-starch polysaccharides content could reliably be determined both in whole flour and in pentosans enriched fractions. Free monosaccharide content was in the range from 0.03% to 0.06% (w/w), while the arabinose/xylose (Ara/Xyl) ratios varied from 0.8 to 1.4 in soluble non-starch polysaccharides and from 0.7 to 0.9 in total non-starch polysaccharides. The different Ara/Xyl ratios found for water extractable and unextractable arabinoxylans clearly indicates different substitution degrees in the polymers. Analysis of the dough rheological parameters in relation to the water soluble and non-soluble non-starch polysaccharides and the Ara/Xyl ratios from different wheat varieties was performed. A clear relation between some of these parameters could be inferred, since a direct relationship between total unextractable (AXi) content and resistance of dough to extension (P), as well an inverse relationship between the same parameter and dough extensibility (L) were observed. These results suggest that the flour non-starch polysaccharide content, as well the Ara/Xyl ratios may be used as additional parameters to estimate some of the wheat flours dough properties.     

Comparison of quality of refined and whole wheat tortillas

The use of whole wheat flour instead of refined flour significantly improves the nutritional profile of flour tortillas. However, whole and refined flours differ in properties and in how they process, thus needing process modifications to get the desired product quality. To understand these differences, refined and whole wheat flours, dough and tortillas were evaluated and compared for physical and rheological properties. Overall, whole wheat flour required more water (59% of flour weight) than refined wheat (53%) to make machineable dough. Refined flour doughs were more extensible and softer than whole wheat flour doughs, thus easier to process. Whole wheat flour tortillas were larger, thinner and less opaque than refined flour tortillas. In general, refined wheat tortillas were more shelf-stable than whole wheat tortillas. Smaller particle size and less fiber in the refined wheat flour mainly contributed to the observed differences. Among the whole wheat samples, tortillas from strong flours had excellent shelf-stability, which must be considered when whole wheat tortillas are processed. This will minimize the need to add vital wheat gluten or other dough strengtheners.     

Scanning Electron Microscopic Study of Dough and Chapati from Gluten-reconstituted Good and Poor Quality Flour

Hard and soft wheat flours, which were used in the study, resulted in good and poor quality chapatis respectively. Gluten was isolated and interchanged among the two whole wheat flours and studied by scanning electron microscopy for its influence on structural characteristics of dough and its relation to chapati-making quality. Microscopic observations clearly indicated that larger gluten strands covered starch granules in hard wheat flour dough, while gluten was short and starch granules exposed in dough prepared from soft wheat flour. Greater film forming ability of gluten in hard wheat flour dough manifested in long and bulky starch strands interwoven with protein matrix in its chapati crumb. Higher moisture retention and starch gelatinization as a consequence of greater film forming ability of gluten in hard wheat flour resulted in pliable and soft textured chapati.