Competitive and allelopathic interference between soybean crop and annual wormwood (Artemisia annua L.) under field conditions

Annual wormwood interference on soybean crop growth and yield may result from competition and allelopathy, which are modulated by crop management. Allelochemicals released by annual wormwood (e.g. artemisinin) may affect the crop directly or indirectly through the effect on the nitrogen fixing symbiont, Bradyrhizobium japonicum. The objectives were (i) to quantify the crop response (i.e. biomass production, nodulation and yield) to weed interference and (ii) to determinate the relative change of competition and allelopathy interferences, when a sublethal dose of herbicide is applied. Two split plot field experiments with three replications were used. The experiment involved a factorial combination of five weed–crop density (soybean/annual wormwood, plants m^?2) levels: D1, pure soybean, 40/0 plants m^?2; D2, 40/2 plants m^?2; D3, 40/4 plants m^?2 and D4, 40/8 plants m^?2, and D5, pure annual wormwood, 0/8 plants m^?2, two activated carbon (allelopathy) levels: C?, with activated carbon (reduced allelopathy) and C+, non activated carbon applied (with allelopathy) and two herbicide levels: H?, untreated and H+, treated with a sub-lethal dose of glyphosate. Activated carbon to adsorb allelochemicals (with and without activated carbon) and glyphosate application (with and no herbicide) were assigned to sub-plots. Increasing weed density did not affect crop biomass at flowering, but changed nodule number and soybean yield with a different pattern depending on carbon and herbicide treatment. Relative crop yield decreased with increasing relative weed biomass. This decrease was particularly drastic when allelopathy was reduced by activated carbon and without herbicide application. The maximum yield losses of 33% in 2006 and 17% in 2007 were observed with the highest weed density (8 plants m^?2). In contrast, without carbon (high allelopathy level), soybean yield remained stable within the explored range of annual wormwood biomass, despite the fact that weed biomass at high densities (D4) was high enough to generate competition. The lack of response to increasing weed density could be related to the indirect effect of allelochemicals interacting with soil microorganisms (i.e. B. japonicum) that positively affected the nodulation (e.g. larger nodules in 2006 and increased nodules biomass due to higher number of roots in 2007 at high densities). With herbicide application, soybean yield of both carbon treatments remained stable when biomass of annual wormwood increased. This research provided strong evidence in support of the existence of positive effect of allelopathic and competitive interactions between annual wormwood and soybean crop under field conditions that may be overridden under herbicide application.     

Effects of ozone on sugar beet grown in open-top chambers

Sugar beet (Beta vulgaris cv. Patriot) plants were grown on field plots and in open-top chambers (OTCs) in two successive years. In the OTC treatments, plants were exposed to charcoal filtered air, unfiltered air or unfiltered air enriched with additional ozone (O₃). Ozone exposure continued for almost 5 months and the 8-h average concentration was raised from 34 to 39 nL L⁻¹ in the ambient air chambers to 62 nL L⁻¹ in the ozone enriched chambers. In both years, the AOT40 exposure index in the ozone enriched chambers exceeded 30 μL L⁻¹ h during the 5-month exposure period compared to 6.5 and 2.9 μL L⁻¹ h in ambient air in 2003 and 2004, respectively. Visible symptoms in the form of small white necrotic flecks appeared in both seasons in the ozone enriched chambers. When the data for both years were analysed statistically, a significant reduction of root yield of 6% and a slight reduction of sugar content were detected. These changes resulted in an overall reduced sugar yield ha⁻¹ of about 9%. Although the sensitivity of sugar beet to ozone is highly variety-dependent, in general this biennial crop appears less sensitive than annual crops such as wheat and potato. Ozone has limited effects on quality parameters in sugar beet, although an increase in α-amino-N content was observed, in agreement with the increased nitrogen content resulting from ozone exposure of wheat and potato.Enclosure within the OTCs increased aboveground biomass but decreased root yield (fresh biomass) and sugar content. These effects were most likely caused by a reduction of radiation by the chamber walls and annulus. The increased temperature in the chambers reduced yield quality by increasing mineral content.     

Long-term P and K fertilisation strategies and balances affect soil availability indices,crop yield depression risk and N use

The last century has seen a large increase of fertiliser use, along with a subsequent rise of crop productivity. However, in many places its intensive use has become a burden to the environment, and legislation has been introduced to restrict nutrient applications. In combination with changing production scenarios as a result of climate change, this means an improved understanding is needed of how low nutrient availability and climatic stress factors affect yields and yield stability.We examined the long-term effects mineral and organic fertilisation on a nutrient-depleted field, and observed large annual variations: depending on the year, average spring barley yields under unfertilised management (U) were between 17–75% lower than the reference N_½P_½K_½ (60–10–60 kg ha⁻¹). Yields increased up to 174% under N₁P₁K₁ (120–20–120 kg ha⁻¹), while animal manure applications at an N availability level corresponding to N₁ were between 79 and 137%. No temporal yield trends could be observed, but long-term changes of Olsen-P and exchangeable K were related to the nutrient balances (inputs-offtake) (r² = 0.60 and 0.59, respectively, P < 0.001).Multiple linear regression analysis was used to examine the effects of the treatments in combination with annual weather variations. The results could be split into two outcomes, 1) a general relation between yields and temperatures for the periods of early spring (P < 0.01, multiple R² = 0.31) and summer (P < 0.001, multiple R² = 0.45), and 2) an interaction between temperature and nutrient applications during crop establishment, leading to a diverse response of relative yields (P < 0.001, multiple R² = 0.64), i.e. relative yield losses under the unfertilised treatment (U) were greater in years with lower spring temperatures, and, conversely, the increased nutrient availability in the fully mineral and organically fertilised treatments could partially alleviate the negative effects.After 13 years of repeated fertilisation, inputs were suspended for a single year and only N was applied to evaluate the residual effects. Yields were significantly affected by the different fertilisation histories (P < 0.001). Likewise, apparent nitrogen recovery tended to improve with previous inputs, but the observations were highly variable.Overall, the analyses agree with the notion that brief periods of stress at a critical stage may significantly affect yields, and confirmed that management of sufficient nutrient availability is critical for maintaining high and stable yields.     

Unequal salt distribution in the root zone increases growth and yield of cotton

Soil salinity is often heterogeneous, yet plant response to unequal salt distribution (USD) in the root zone is seldom studied in cotton (Gossypium hirsutum L.). Our objective was to evaluate the effects of USD on growth and yield, as well as its potential application for increasing cotton production. To achieve this objective, greenhouse and field experiments were conducted. In the first experiment, potted cotton plants were grown in a split-root system in the greenhouse. Each root half was irrigated with either the same or two concentrations of NaCl. Plant biomass, leaf chlorophyll (Chl), photosynthesis (Pn) and transpiration (Tr), Na⁺ and K⁺ accumulation, as well as biological and economic yields were determined. In the second experiment, plants were grown in furrow-beds in saline fields with those grown on flat beds as controls. Root-zone salinity, yield and yield components and earliness (the percentage of the first two harvests to total harvests) were monitored. When the entire root system was exposed to the same concentration of NaCl, shoot dry weight, leaf area, plant biomass, leaf Chl, Pn and Tr were markedly reduced relative to the NaCl-free control at 2 weeks after salinity stress (WAS). Significant reductions in biological (23.6–73.8%) and economic yields (38.1–79.7%) were noticed at harvest. However, when only half of the root system was exposed to low-salinity, the inhibition effect of salinity on growth and yield was significantly reduced. Plant biomass and seed cotton yield were increased by 13 and 23.9% with 50/150 mM/mM NaCl, 40 and 44.5% with 100/300 mM/mM NaCl, and 85.7 and 127.8% with 100/500 mM/mM NaCl relative to their respective equal salt distribution (ESD) controls (100/100, 200/200, and 300/300). Unequal salt distribution also decreased concentrations of Na⁺ and increased leaf K⁺ and Chl content, K⁺/Na⁺ ratio, Pn and Tr, compared with ESD. Furrow-bed seeding induced unequal distribution of salts in the surface soil during the field experiment. Under furrow planting, soil salinity was much higher, but soil osmotic potential was much lower on the ridged part than the furrows. Yield and earliness were increased 20.8 and 5.1% by furrow seeding relative to flat seeding. These enhancements were mainly attributed to unequal distribution of salts in the root zone. Thus, specific cultural practices that induce unequal salt distribution such as furrow-bed seeding can be used to improve cotton production in saline fields.     

Effects of reduced nitrogen input on productivity and N2O emissions in a sugarcane/soybean intercropping system

A seven-year (2009–2015) continuous field experiment was established at the South China Agricultural University in order to identify the effects of sugarcane/soybean intercropping and reduced N rate on ecosystem productivity, yield stability, soil fertility, and N₂O emissions. The randomized block experiment was designed with four cropping patterns (sugarcane monocropping (MS), soybean monocropping (MB), sugarcane/soybean (1:1) intercropping (SB1), and sugarcane/soybean (1:2) intercropping (SB2)) and two rates of N fertilization (300 kg hm⁻² (N1, reduced rate) and 525 kg hm⁻² (N2, conventional rate)). The results showed that the land equivalent ratio (LER) of all intercropping systems was greater than 1 (between 1.10 and 1.84), and the SB2-N1 optimally improved the land utilization rate among all treatments. The cropping patterns and N applied rates had no significant effect on sugarcane yield. The soybean yield was influenced by different cropping patterns because of different planting densities (4, 8 and 16 rows of soybean were plant under SB1, SB2, and MB, respectively) and was adopted in this experiment. In addition, under the SB2 cropping pattern, the soybean yield at the reduced N application rate was higher than that at the conventional N application rate. Wricke’s ecovalence (W_i²), the sustainable yield index (SYI) and the coefficient of variation (CV) were used to evaluate yield stability. Different treatments had no significant effects on sugarcane yield stability, as demonstrated by three indicators (W_i², SYI and CV), which indicated that intercropping with soybean and reduced N rate had no effect on sugarcane yield. For soybeans, the value of W_i² demonstrated that the stability of the intercropping system was higher than its counterpart monocropping system, as SYI and CV values indicated that SB2 had higher stability than SB1. During seven years of experiments, there was no significant difference in the soil fertility between MS and SB patterns. The soybean monocropping had a higher available K, pH and lower available P content than sugarcane inter- and mono-cropping. Different cropping patterns had a slight impact on N₂O emissions and the greenhouse gas intensity (GHGI) value. Higher N input promoted N₂O emissions and increased GHGI values. In conclusion, the present study observed that a 40% reduced nitrogen input combined with intercropping soybeans could maintain sugarcane yield and soil sustainable utilization, and that higher N fertilizer additions induced negative impacts on greenhouse gases emissions. Sugarcane intercropping with soybeans can reduce chemical fertilizer input and simultaneously maintain crop productivity; thus, it can be considered to be a reasonable practice for field management.     

Ultraviolet-B radiation and nitrogen effects on growth and yield of maize under Mediterranean field conditions

The effects of an increase in UV-B radiation on growth and yield of maize (Zea mays L.) were investigated at four levels of applied nitrogen (0, 100, 200 and 300 kg ha⁻¹ of N) under Mediterranean field conditions. The experiment simulated a 20% stratospheric ozone depletion over Portugal. Enhanced UV-B and N deficiency decreased yield and total biomass production by 22–49%. High UV-B dose reduced yield, total biomass and growth of N-fertilized maize plants but did not affect N-stressed plants to the same extent. The response of grain yield to N was smaller with enhanced UV-B radiation. The underlying mechanisms for these results are discussed.     

Radiation use efficiency,chemical composition,and methane yield of biogas crops under rainfed and irrigated conditions

For biomethane production, the cup plant (Silphium perfoliatum L.) is considered a promising alternative substrate to silage maize (Zea mays L.) due to its high biomass potential and associated ecological and environmental benefits. It has also been suggested to grow cup plant on less productive soils because of its presumed drought tolerance, but robust information on the impact of water shortage on biomass growth and substrate quality of cup plant is rare. Therefore, this study assesses the effects of soil water availability on the chemical composition and specific methane yield (SMY) of cup plant. Furthermore above-ground dry matter yield (DMY) was analysed as a function of intercepted photosynthetic active radiation (PAR) and radiation use efficiency (RUE). Data were collected in a two-year field experiment under rainfed and irrigated conditions with cup plant, maize, and lucerne-grass (Medicago sativa L., Festuca pratensis Huds., Phleum pratense L.). The cup plant revealed a slight decrease of −6% in the SMY in response to water shortage (less than 50% of plant available water capacity). The average SMY of cup plant [306 l (kg volatile solids (VS))⁻¹] was lower than that of maize [362 l (kg VS)⁻¹] and lucerne-grass [334 l (kg VS)⁻¹]. The mean drought-related reduction of the methane hectare yield (MHY) was significantly greater for cup plant (−40%) than for maize (−17%) and lucerne-grass (−13%). The DMY reduction in rainfed cup plant was mainly attributed to a more severe decrease in RUE (−29%) than for maize (−16%) and lucerne-grass (−12%). Under water stress, the mean cup plant RUE (1.3 g MJ⁻¹) was significantly lower than that of maize (2.9 g MJ⁻¹) and lucerne-grass (1.4 g MJ⁻¹). Compared to RUE, the reduced PAR interception was less meaningful for DMY in rainfed crops. Hence, the cup plant is not suitable for growing on drought prone lands due to its high water demand required to produce reasonably high MHYs.     

Tree-crop interactions in maize-eucalypt woodlot systems in southern Rwanda

We studied the interaction between Eucalyptus saligna woodlots and maize crop in southern Rwanda. Three sites were selected and in each, a eucalypt woodlot with mature trees and a suitable adjoining crop field of 12.75 m × 30 m was selected. This was split into two plots of 6 m × 12 m and further subdivided into nine sub-plots running parallel to the tree-crop interface. Maize was grown in both 6 m × 12 m plots and one of these received fertiliser. Soil moisture, nutrients and solar radiation were significantly reduced near the woodlots, diminishing grain yield by 80% in the 10.5 m crop-field strip next to the woodlot. This reduction however affects only 10.5% of the maize crop field, leaving 89.5% unaffected. Spreading the loss to a hectare crop field, leads to an actual yield loss of 0.21 t ha⁻¹, equivalent to 8.4%. Expressing yield loss in tree-crop systems usually presented as a percentage of yield recorded near the trees to that obtained in open areas may be misleading. Actual yields should be reported with corresponding crop field areas affected. Variation in grain yield coincided with those for soil moisture, soil N and K; all increasing from the woodlot-maize interface up to 10.5 m and remaining similar to the values in open areas thereafter. Solar radiation continued to increase with distance up to 18 m from the woodlot-maize interface. Harvest index in unfertilised maize exceeded that in the fertilised treatment reflecting the crop’s strategy to allocate resources to grain production under unfavourable conditions. Fertilisation increased maize yield from 1.3–2.6 t ha⁻¹ but the trend in the woodlot effects on maize remained unaltered.     

The effects of ethylene, 1-MCP and AVG on sprouting in sweetpotato roots

Exogenous ethylene is commonly used as a commercial sprouting inhibitor of potato tubers. The role of ethylene in the control of sprouting of sweetpotato roots, however, is not known. The aim of this study was to investigate the role of ethylene in control of sprouting in sweetpotato roots by observing the effect of an ethylene synthesis inhibitor, aminoethoxyvinylglycine (AVG), and the ethylene antagonist, 1-methylcyclopropene (1-MCP), in the presence and absence of exogenous ethylene on root sprouting and associated sugar accumulation. Continuous exposure to 10 μl L⁻¹ ethylene, 24 h exposure to 625 nl L⁻¹ 1-MCP or dipping in 100 μl L⁻¹ AVG all inhibited sprout growth in sweetpotato roots of two varieties over 4 weeks of storage at 25 °C. The observations that both ethylene on its own and 1-MCP, which inhibits ethylene action, inhibit sprout growth indicate that while continuous exposure to exogenous ethylene leads to sprout growth inhibition, ethylene is also required for sprouting. In potato tubers ethylene is required to break dormancy, while continuous exposure inhibits sprout growth.Monosaccharide concentrations in ethylene, 1-MCP or AVG treated roots were lower than in untreated roots, and for ethylene treated roots this was associated with higher respiration rates. This is consistent with the activation of some additional process by ethylene which uses energy through sugar metabolism. 1-MCP and AVG both inhibited this increase in respiration rate and counteracted the decrease in monosaccharide concentrations. 1-MCP presumably counteracts the ethylene stimulation of this process, while the effect of AVG is attributed to its possible inhibitory effects on protein synthesis.     

The new nitrification inhibitor 3,4-dimethylpyrazole succinic (DMPSA) as an alternative to DMPP for reducing N2O emissions from wheat crops under humid Mediterranean conditions

Nowadays agricultural practices are based in the use of N fertilizers which can lead to environmental N losses. These losses can occur as nitrous oxide (N₂O) emissions as result of the microbial processes of nitrification and denitrification. N₂O together with carbon dioxide (CO₂) and methane (CH₄) are the strongest greenhouse gases (GHG) associated with agricultural soils. Nitrification inhibitors (NI) have been developed with the aim of decreasing fertilizer-induced N losses and increasing N efficiency. One of the most popular NI is the 3,4-dimethylpyrazol phosphate (DMPP) which have proven to be an advisable strategy to mitigate GHG emissions while maintaining crops yield. A new NI, 3,4-dimethylpyrazole succinic (DMPSA), has been developed. The objective of this study was to compare the impact of the new nitrification inhibitor DMPSA on greenhouse gases emissions, wheat yield and grain protein with respect to DMPP. For this purpose a field-experiment was carried out for two years. Fertilizer dose, with and without NIs, was 180 kg N ha⁻¹ applied as ammonium sulphate (AS) split in two applications of 60 kg N ha⁻¹ and 120 kg N ha⁻¹, respectively. A single application of 180 kg N ha⁻¹ of AS with NIs was also made. An unfertilized treatment was also included. The new nitrification inhibitor DMPSA reduces N₂O emissions up to levels of the unfertilized control treatment maintaining the yield and its components. The DMPSA shows the same behavior as DMPP in relation to N₂O fluxes, as well as wheat yield and quality. In spite of applying a double dose of N at stem elongation than at tillering, N₂O losses from that period are lower than at tillering as a consequence of the influence of soil water content and temperature reducing the N₂O/N₂ ratio by denitrification. NI efficiency in reducing N₂O losses is determined by the magnitude of the losses from the AS treatment.     

Effects of irrigation and nitrogen fertilization on the greenhouse gas emissions of a cropping system on a sandy soil in northeast Germany

Irrigation induces processes that may either decrease or increase greenhouse gas emissions from cropping systems. To estimate the net effect of irrigation on the greenhouse gas emissions, it is necessary to consider changes in the crop yields, the content of soil organic carbon and nitrous oxide emissions, as well as in emissions from the use and production of machinery and auxiliary materials. In this study the net greenhouse gas emissions of a cropping system on a sandy soil in northeast Germany were calculated based on a long-term field experiment coupled with two-year N₂O flux measurements on selected plots. The cropping system comprised a rotation of potato, winter wheat, winter oil seed rape, winter rye and cocksfoot each under three nitrogen (N) fertilization intensities with and without irrigation. Total greenhouse gas emissions ranged from 452 to 3503 kg CO_2-eq ha⁻¹ and 0.09 to 1.81 kg CO_2-eq kg⁻¹ yield. Application of an adequate amount of N fertilizer led to a decrease in greenhouse gas emissions compared to zero N fertilization whereas excessive N fertilization did not result in a further decrease. Under N fertilization there were no significant differences between irrigation and non-irrigation. Increases in greenhouse gas emissions from the operation, production and maintenance of irrigation equipment were mainly offset by increases in crop yield and soil organic carbon contents. Thus, on a sandy soil under climatic conditions of north-east Germany it is possible to produce higher yields under irrigation without an increase in the yield-related greenhouse gas emissions.     

Effects of planting soybean in summer fallow on wheat grain yield,total N and Zn in grain and available N and Zn in soil on the Loess Plateau of China

Dryland wheat is the major contributor to wheat production in the world, where water deficiency and poor soil fertility are key factors limiting wheat grain yields and nutrient concentrations. A field experiment was carried out from June 2008 to June 2011 at Shilipu (latitude 35.12°N, longitude 107.45°E and altitude 1200 m above sea level) on the Loess Plateau (a typical dryland) in China, to investigate the effects of rotation with soybean (Glycine max) green manure (GM) on grain yield, total N and total Zn concentrations in subsequent wheat (Triticum aestivum L.), and on nitrate-N and available Zn in the soil. The benefits of crop rotation with soybean GM on wheat grain yields became more evident with time. In the second and third years, the grain yields of wheat rotated with soybean GM reached 4871 and 5089 kg ha⁻¹ at the 108 kg N ha⁻¹ rate. These yields were 21% and 12% higher than the highest yields of wheat under a fallow-winter wheat (FW) rotation. Rotation with soybean GM reduced the amount of N fertilizer required to obtain wheat grain yields and biomass levels similar to wheat grown in the FW rotation by 20–33%. In the first 2 years, average grain N concentrations over all N rates increased by 6% and 12%, and those of Zn increased by 26% and 14% under the soybean GM-winter wheat (SW) rotation, compared with the FW rotation. The increased grain N and Zn concentrations were found to be related to the increased concentrations of nitrate-N and available Zn in the soil, particularly at the sowing of winter wheat. However, grain N and Zn concentrations were not improved by rotation with soybean GM in the third year. This was attributed to the dilution effect caused by the more grain yield increase than its nutrient export. In conclusion, planting soybean for GM in fallow fields reduced the need for N fertilizer to enhance wheat yields in this dryland region. Change in wheat grain N and Zn concentrations was related to soil nutrient concentrations, and to the balance between increased grain yield and its nutrient export.     

Assessment of the benefits of frost-sensitive companion plants in winter rapeseed

The intercropping of rapeseed with frost-sensitive companion plants (CP) has recently been proposed as a way to mitigate the negative environmental impact of rapeseed crops. Using mixed-effect linear models, we compared the yield and weed amounts of rapeseed intercropped with different CP species with that of rapeseed as a sole crop in an unique dataset of 79 field experiments covering a wide range of climate, soil and practices conditions in the northwestern part of France, from 2009 to 2015. Bayesian model averaging procedure was used to determine the relative contributions of sites characteristics to the effects of intercropping.Before winter, field pea and faba bean had accumulated the largest amounts of dry mass, with more than 100 g m⁻². Rapeseed biomass was reduced by 56% by non-legume CPs and by only 18% by legume CPs, the largest decrease being caused by pea. Non-legumes decreased the nitrogen nutrition index of rapeseed by 7%, whereas pea and faba bean increased this index by 6% and 3%, respectively. Intercropping with non-legume and legume CPs reduced weed amounts by 52% and 38% respectively, with no difference between CP species. Non-legume CPs decreased rapeseed yield at harvest by 0.58 t ha⁻¹, whereas faba bean and faba bean + lentil increased yield by 0.16 and 0.12 t ha⁻¹ respectively, when fertilized at the recommended rate. Intercropping with faba bean, lentil or a mixture of both made it possible to reduce nitrogen applications by 30–40 kg ha⁻¹ with no significant decrease in rapeseed yield. Faba bean and faba bean + lentil mixtures had the best overall performance. This work suggests that intercropping rapeseed is promising, particularly in soils with low nitrogen content with an early sowing date in the late summer.     

Effect of abscisic acid applications on cold tolerance in chickpea (Cicer arietinum L.)

A series of field experiments were undertaken at three locations in Khyber PukhtunKhwa (KPK) Province, Pakistan to assess the effects of low temperatures and phytohormone applications on chickpea (Cicer arietinum L.) growth and yield. These trials showed that ABA application (10⁻⁴ M) to 40-day-old plants (before the first seasonal frost) offset low temperature-induced growth and yield depression at harvest (200-day-old plants) by up to 17%. These yield improvements were mainly due to an increase in the number of seeds pod⁻¹. Growth room experiments were carried out under controlled environmental conditions to establish how foliar application of 10⁻⁴ M ABA to 40-day-old plants might improve seed production at harvest. The foliar application of 10⁻⁴ M ABA had no detectable effect on endogenous shoot or root ABA levels four-days after spraying or on biomass when plants were maintained in warm conditions. When exposed to night temperatures of −2 °C, however, the endogenous ABA levels increased dramatically in both control and ABA-treated plants, but this rise was more rapid after ABA application (p < 0.01); after 14 days, these plants had gained significantly more biomass than the unsprayed controls (p < 0.05). No evidence was found to suggest ABA affected the osmotic or water balance of plants, but parallel experiments have shown ABA reduced low temperature-induced cell damage. Analysis of the proteome of the shoot tissues of ABA treated and untreated plants by 2-Dimensional Gel Electrophoresis identified several proteins that are induced by low temperatures and/or by ABA application in chickpea and which may be involved in conferring cold tolerance. Attempts were made to establish the identity of these proteins using mass spectrometry but in all cases the results were ambiguous; a more complete protein data base for legumes is required before the function of these proteins can be inferred.     

Growth and yield of winter wheat in the first 3 years of a monoculture under varying N fertilization in NW Germany

Different preceding crops interact with almost all husbandry and have a major effect on crop yields. In order to quantify the yield response of winter wheat, a field trial with different preceding crop combinations (oilseed rape (OSR)–OSR–OSR–wheat–wheat–wheat), two sowing dates (mid/end of September, mid/end of October) and 16 mineral nitrogen (N) treatments (80–320 kg N ha⁻¹) during 1993/1994–1998/1999, was carried out at Hohenschulen Experimental Station near Kiel in NW Germany. Single plant biomass, tiller numbers m⁻², biomass m⁻², grain yield and yield components at harvest were investigated. During the growing season, the incidence of root rot (Gaeumannomyces graminis) was observed. Additionally, a bioassay with Lemna minor was used to identify the presence of allelochemicals in the soil after different preceding crops.Averaged over all years and all other treatments, wheat following OSR achieved nearly 9.5 t ha⁻¹, whereas the second wheat crop following wheat yielded about 0.9 t ha⁻¹ and the third wheat crop following 2 years of wheat about 1.9 t ha⁻¹ less compared with wheat after OSR. A delay of the sowing date only marginally decreased grain yield by 0.2 t ha⁻¹. Nitrogen fertilization increased grain yield after all preceding crop combinations, but at different levels. Wheat grown after OSR reached its maximum yield of 9.7 t ha⁻¹ with 210 kg N ha⁻¹. The third wheat crop required a N amount of 270 kg N ha⁻¹ to achieve its yield maximum of 8.0 t ha⁻¹.Yield losses were mainly caused by a lower ear density and a reduced thousand grain weight. About 4 weeks after plant establishment, single wheat plants following OSR accumulated more biomass compared to plants grown after wheat. Plants from the third wheat crop were smallest. This range of the preceding crop combinations was similar at all sampling dates throughout the growing season.Root rot occurred only at a low level and was excluded to cause the yield losses. The Lemna bioassay suggested the presence of allelochemicals, which might have been one reason for the poor single plant development in autumn.An increased N fertilization compensated for the lower number of ears m⁻² and partly reduced the yield losses due to the unfavorable preceding crop combination. However, it was not possible to completely compensate for the detrimental influences of an unfavorable preceding crop on the grain yield of the subsequent wheat crop.     

Tillage,cover crops,and nitrogen fertilization effects on soil nitrogen and cotton and sorghum yields

Sustainable soil and crop management practices that reduce soil erosion and nitrogen (N) leaching, conserve soil organic matter, and optimize cotton and sorghum yields still remain a challenge. We examined the influence of three tillage practices (no-till, strip till and chisel till), four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secaele cereale L.)], vetch/rye biculture and winter weeds or no cover crop}, and three N fertilization rates (0, 60–65 and 120–130 kg N ha⁻¹) on soil inorganic N content at the 0–30 cm depth and yields and N uptake of cotton (Gossypium hirsutum L.) and sorghum [Sorghum bicolor (L.) Moench]. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic, Plinthic Paleudults) from 1999 to 2002 in Georgia, USA. Nitrogen supplied by cover crops was greater with vetch and vetch/rye biculture than with rye and weeds. Soil inorganic N at the 0–10 and 10–30 cm depths increased with increasing N rate and were greater with vetch than with rye and weeds in April 2000 and 2002. Inorganic N at 0–10 cm was also greater with vetch than with rye in no-till, greater with vetch/rye than with rye and weeds in strip till, and greater with vetch than with rye and weeds in chisel till. In 2000, cotton lint yield and N uptake were greater in no-till with rye or 60 kg N ha⁻¹ than in other treatments, but biomass (stems + leaves) yield and N uptake were greater with vetch and vetch/rye than with rye or weeds, and greater with 60 and 120 than with 0 kg N ha⁻¹. In 2001, sorghum grain yield, biomass yield, and N uptake were greater in strip till and chisel till than in no-till, and greater in vetch and vetch/rye with or without N than in rye and weeds with 0 or 65 kg N ha⁻¹. In 2002, cotton lint yield and N uptake were greater in chisel till, rye and weeds with 0 or 60 kg N ha⁻¹ than in other treatments, but biomass N uptake was greater in vetch/rye with 60 kg N ha⁻¹ than in rye and weeds with 0 or 60 kg N ha⁻¹. Increased N supplied by hairy vetch or 120–130 kg N ha⁻¹ increased soil N availability, sorghum grain yield, cotton and sorghum biomass yields, and N uptake but decreased cotton lint yield and lint N uptake compared with rye, weeds or 0 kg N ha⁻¹. Cotton and sorghum yields and N uptake can be optimized and potentials for soil erosion and N leaching can be reduced by using conservation tillage, such as no-till or strip till, with vetch/rye biculture cover crop and 60–65 kg N ha⁻¹. The results can be applied in regions where cover crops can be grown in the winter to reduce soil erosion and N leaching and where tillage intensity and N fertilization rates can be minimized to reduce the costs of energy requirement for tillage and N fertilization while optimizing crop production.     

The effect of organic and conventional management on the yield and quality of wheat grown in a long-term field trial

The performance of winter wheat was evaluated under organic (ORG) and conventional (CON) management systems in the Nafferton Factorial Systems Comparison (NFSC) long-term field trial. The present study separates out the crop protection and fertility management components of organic and conventional production systems using two levels each of crop protection (CP) and fertility management (FM). The experimental design provided the four combinations of crop protection and fertility (CON-CP CON-FM, CON-CP ORG-FM, ORG-CP CON-FM and ORG-CP ORG-FM) to evaluate their effects on yield, quality (protein content and hectolitre weight) and disease levels during the period 2004–2008. The conventional management system (CON-CP CON-FM) out-yielded the organic management system (ORG-CP ORG-FM) in all years by an average of 3.1 t ha⁻¹, i.e. 7.9 t ha⁻¹ vs. 4.8 t ha⁻¹. Fertility management was the key factor identified limiting both yield and grain protein content in the ORG management system. The CON-FM produced on average a 3% higher protein content than ORG-FM in all years (12.5% vs. 9.7%). However the ORG-CP system produced higher protein levels than CON-CP although it was only in 2008 that this was statistically significant. In contrast to protein content it was ORG-FM which produced a higher hectolitre weight than the CON-FM system (71.6 kg hl⁻¹ vs. 71.0 kg hl⁻¹). The clear and significant differences in yield and protein content between the ORG-FM and CON-FM systems suggest a limited supply of available N in the organic fertility management system which is also supported by the significant interaction effect of the preceding crop on protein content. The pRDA showed that although fertilisation had the greatest effect on yield, quality and disease there was also a considerable effect of crop protection and the environment.     

Use of soil and vegetation spectroradiometry to investigate crop water use efficiency of a drip irrigated tomato

An agronomic research was conducted in Tuscany (Central Italy) to evaluate the effects of an advanced irrigation system on the water use efficiency (WUE) of a tomato crop and to investigate the ability of soil and vegetation spectroradiometry to detect and map WUE. Irrigation was applied following an innovative approach based on CropSense system. Soil water content was monitored at four soil depths (10, 20, 30 and 50 cm) by a probe. Rainfall during the crop cycle reached 162 mm and irrigation water applied with a drip system amounted to 207 mm, distributed with 16 irrigation events. Tomato yield varied from 7.10 to 14.4 kg m⁻², with a WUE ranging from 19.1 to 38.9 kg m⁻³. The irrigation system allowed a high yield levels and a low depth of water applied, as compared to seasonal ET crop estimated with Hargraves’ formula and with the literature data on irrigated tomato. Measurements were carried out on geo-referenced points to gather information on crop (crop yield, eighteen Vegetation indices, leaf area index) and on soil (spectroradiometric and traditional analysis). Eight VIs, out of nineteen ones analyzed, showed a significant relationship with georeferenced yield data; PVI maps seemed able to return the best response, before harvesting, to improve the knowledge of the area of cultivation and irrigation system. CropSense irrigation system reduced seasonal irrigation volumes. Some vegetation indexes were significantly correlated to tomato yield and well identify, a posteriori, crop area with low WUE; spectroradiometry can be a valuable tool to improve irrigated tomato field management.     

Influence of sulfur on apparent N-use efficiency,yield and quality of oilseed rape (Brassica napus L.) grown on a calcareous soil

In the Lorraine region, major soils used for winter oilseed rape are calcareous. Across two pot and two field experiments, we studied the influence of sulfur applied at different levels on apparent N-use efficiency (ANU), yield, glucosinolate (GLS) and oil content of seeds. The soil received a constant dose of 200 kg N ha⁻¹ as ammonium nitrate, urea or cow slurry and three levels of S: 0, 30 and 75 kg ha⁻¹ as ammonium thiosulfate (ATS), MgSO₄ or ATS plus MgSO₄. Apparently, oilseed rape is a N-inefficient crop as revealed by low ANU values which varied within 36 and 53% from field experiment versus 25 and 61% under controlled conditions. In both cases, S additions improved N-use efficiency only at the highest dose of 75 kg S ha⁻¹, which is not attained by ATS with 35 kg S ha⁻¹ (10% v/v). S fertilization increased the GLS contents that were found to be negatively correlated with plant N/S uptake ratios observed at maturity. The most important increase in GLS content by 52% was noted with cow slurry in the pot experiment. But, as a whole, the GLS levels remain below the European norm of 18 μmol g seed⁻¹. Moreover, the oil content (% DM) of seeds decreased (but the total production increased) when the soil was fertilized with N and with or without S. The results showed that N and S nutrition during the growth were tightly linked. Their interactions, as reflected by plant uptake, are synergistic at optimum rates and antagonistic at excessive levels of one of the both. Collectively, the results indicate that S fertilization is required to improve N-use efficiency and thereby maintaining a sufficient oil level and fatty acid quality.     

Performance and application of the APSIM Nwheat model in the Netherlands

APSIM Nwheat is a crop system simulation model, consisting of modules that incorporate aspects of soil water, nitrogen (N), crop residues, and crop growth and development. The model was applied to simulate above- and below-ground growth, grain yield, water and N uptake, and soil water and soil N of wheat crops in the Netherlands. Model outputs were compared with detailed measurements of field experiments from three locations with two different soil types. The experiments covered two seasons and a range of N-fertiliser applications. The overall APSIM Nwheat model simulations of soil mineral N, N uptake, shoot growth, phenology, kernels m⁻², specific grain weight and grain N were acceptable. Grain yields (dry weight) and grain protein concentrations were well simulated with a root mean square deviation (RMSD) of 0.8 t ha⁻¹ and 1.6 protein%, respectively. Additionally, the model simulations were compared with grain yields from a long-term winter wheat experiment with different N applications, two additional N experiments and regional grain yield records. The model reproduced the general effects of N treatments on yields. Simulations showed a good consistency with the higher yields of the long-term experiment, but overpredicted the lower yields. Simulations and earlier regional yields differed, but they showed uniformity for the last decade.In a simulation experiment, the APSIM Nwheat model was used with historical weather data to study the relationship between rate and timing of N fertiliser and grain yield, grain protein and soil residual N. A median grain yield of 4.5 t ha⁻¹ was achieved without applying fertiliser, utilising mineral soil N from previous seasons, from mineralisation and N deposition. Application of N fertiliser in February to increase soil mineral N to 140 kg N ha⁻¹ improved the median yield to 7.8 t ha⁻¹ but had little effect on grain protein concentration with a range of 8–10%. Nitrogen applications at tillering and the beginning of stem elongation further increased grain yield and in particular grain protein, but did not affect soil residual N, except in a year with low rainfall during stem elongation. A late N application at flag leaf stage increased grain protein content by several per cent. This increase had only a small effect on grain yield and did not increase soil residual N with up to 40 kg N ha⁻¹ applied, except when N uptake was limited by low rainfall in the period after the flag leaf stage. The economic and environmental optima in winter wheat were identified with up to 140 kg N ha⁻¹ in February, 90 kg N ha⁻¹ between tillering and beginning of stem elongation and 40 kg N ha⁻¹ at flag leaf stage resulting in a median of 8.5 t ha⁻¹ grain yield, 14.0% grain protein and 13 kg N ha⁻¹ soil residual N after the harvest. The maximum simulated yield with maximum N input from two locations in the Netherlands was 9.9 t ha⁻¹.