Iodine biofortification in tomato

Iodine is an essential element in the human diet, and iodine deficiency is a significant health problem. No attempts to increase iodine content in plant‐derived food (biofortification) have so far been particularly effective. We studied iodine uptake in tomato (Solanum lycopersicum L.) to evaluate whether it is possible to increase the iodine concentration in its fruits. Iodine translocation and storage inside tomato tissues were studied using radioactive iodine. Potassium iodide was also supplied at different concentrations to tomato plants to evaluate the resulting iodide concentration both in the vegetative tissues and the fruits. The results indicate that iodine was taken up better when supplied to the roots using hydroponically grown plants. However, a considerable amount of iodine was also stored after leaf treatment, suggesting that iodine transport through phloem also occurred. We found that tomato plants can tolerate high levels of iodine, stored both in the vegetative tissues and fruits at concentrations that are more than sufficient for the human diet. We conclude that tomato is an excellent crop for iodine‐biofortification programs.     

Influence of Foliar and Ground Fertilization on Yield,Fruit Quality,and Soil,Leaf, and Fruit Mineral Nutrients in Apple

ABSTRACT

The effectiveness of nitrogen (N)+ zinc (Zn) soil and foliar fertilizer applications on growth, yield, and quality of apple (Malus domestic Borkh ‘Golden Delicious’) fruit was studied in the Zanjan province, Iran. There were eight treatments 1) control (no fertilizer), 2) soil applied N, 3) soil applied Zn, 4) soil applied N+Zn, 5) foliar applied N, 6) foliar applied Zn, 7) foliar applied N+Zn and 8) combined soil and foliar applied N+Zn. The N source was urea [CO(NH₂)₂, 46% N] applied at 276 N tree^{? 1} yr^?1 and the Zn source was zinc sulfate (ZnSO₄,7H₂0, 23% Zn) applied at 110 g Zn tree^{? 1} yr^{? 1}. The soil treatments of N and Zn, were applied every two weeks during June through August (total of 6 times/year) in a 1 m radius around the tree trunk (drip line of trees). The foliar solutions of N (10 g l^{? 1} urea) and Zn [8 g l^{? 1} zinc sulfate (ZnSO₄)] were sprayed at the rate of 10 L tree^{? 1} every two weeks at the same times as described for soil applications. The highest yield (49 kg tree^{? 1}), and the heaviest fruits (202 g) were obtained in the soil and foliar combination of N+Zn treatment. The lowest yield (35 kg tree^{? 1}), and the smallest fruits (175 g) were recorded in the control. Nitrogen, and to a lesser extent Zn, foliar application resulted in decreasing fruit quality (caused russeting, and lower soluble solid), but increasing N leaf and fruit concentrations (2.4% DW and 563 mg kg^{? 1}, respectively). There were significant differences among yield and leaf mineral nutrient concentration in different treatments. But there was no significant difference between fruit mineral nutrient concentration (except N). Ratio of N/calcium (Ca), potassium (K)/Ca, and [magnesium (Mg)+K]/Ca in fruits were found suitable for fruit quality prediction. Combining the zinc sulfate with urea in the foliar applications increased the concentration of Zn from 0.7 to 1.5 mg per kg of apple tissue. Leaf N concentration varied during growth season. Foliar applied nutrient can be more efficient than soil applied, but a combination of soil and foliar applications is recommended for apple tree nutrient management.     

Zinc fertilization approaches for agronomic biofortification and estimated human bioavailability of zinc in maize grain

Maize (Zea mays L.) is generally low in bioavailable zinc (Zn); however, agronomic biofortification can cure human Zn deficiency. In the present experiment, Zn was applied in pots as ZnSO₄ · 7H₂O to maize cultivar DK-6142 as foliar spray (0.5% w/v Zn sprayed 25 days after sowing and 0.25% w/v at tasseling), surface broadcasting (16 kg Zn ha^?1), subsurface banding (16 kg Zn ha^?1 at the depth of 15 cm), surface broadcasting + foliar and subsurface banding + foliar in comparison to an unfertilized control. As compared to control, all treatments significantly (P ≤ 0.05) increased growth, yield and nutritional attributes in maize. Grain Zn and protein concentrations were correlated and ranged from 22.3 to 41.9 mg kg^?1 and 9 to 12 %, respectively. Zinc fertilization also significantly reduced grain phytate and increased grain Zn concentration. Zinc fertilization, especially broadcasting and subsurface banding combined with foliar spray decreased grain [phytate]:[Zn] ratio to 28 and 21 and increased Zn bioavailability by trivariate model of Zn absorption to 2.04 to 2.40, respectively. Conclusively, broadcasting and subsurface banding combined with foliar spray is suitable for optimal maize yield and agronomic Zn biofortification of maize grain. This would also be helpful to optimize Zn and protein concentration in maize grain.     

Response of Mature Phosphorus-Deficient Apple Trees to Phosphorus Fertilization and Liming

ABSTRACT

The aim of the study was to examine response of mature phosphorus (P) deficient apple (Malus domestica Borkh.) trees to phosphorus fertilization and liming. The experiment was carried out during 2003–2005 in a commercial orchard in Central Poland on ‘Jonagold’ apple trees/M.26 planted in 1996 on a coarse-textured soil with low both pH (4.6) and organic matter (1.2%). Calcium-lactate soluble phosphorus concentration in the soil was within an optimal range despite appearance of leaf phosphorus deficiency symptoms. Soil and foliar applications of phosphorus, and soil liming were applied. Soil phosphorus fertilization was made in the first year of the experimental at a rate of 100 kg P per ha as triple superphosphate. Foliar sprays of a soluble compound containing organic phosphorus were performed 5 times per season at 2-week intervals, starting 4 weeks after full bloom. Soil liming was applied in the fall 2002 at a rate of 1100 kg Ca ha^?1 as hydrated lime. Additional combination as soil phosphorus fertilization plus liming was also applied. Plots unsupplied with phosphorus and lime served as a control. The results showed that liming and liming plus soil P application increased soil pH, and phosphatase activity in the soil, and improved phosphorus nutrition, tree vigor, yield, fruit color, and firmness after storage; effect of these treatments was not found only in the first year of the study. In all years foliar phosphorus sprays improved phosphorus nutrition of apple trees, and fruit color and firmness after storage. In 2 out of 3 years foliar phosphorus application increased yield. The vegetative and reproductive responses of ‘Jonagold’ apple trees did not depend on soil phosphorus fertilization. It was concluded that maintaining an optimal pH of soils for apple trees limits the incidence of orchard phosphorus deficiency and that foliar phosphorus sprays should be applied in phosphorus-deficient apple orchards to improve yield, and fruit appearance and storability.     

Effect of foliar fertilization of potassium on yield,quality, and nutrient uptake of groundnut

Abstract

Groundnut (Arachis hypogae L.) is the most important oilseed crop of India and it is abundantly grown under rainfed conditions in vertisols of Western India. The objective of this work was to study the effect of potassium (K) basal and foliar fertilization on yield, nutrient concentration in tissue and quality parameters of groundnut. Two varieties, GAUG‐1 (bunch type) and GAUG‐10 (spreading type) were grown during Kharif (rainfed) and Rabi (irrigated) seasons at Junagadh, Gujarat. The experiment compared two foliarapplied K fertilizers (KCl and K₂SO₄) at two different doses (0.5 and 1.0%) with basal KCl application (0 and 50 kg K₂O ha^?1). Field soil was highly calcareous (pH 8.2, NH₄OAc extractable K 188 kg ha^?1 with 40% lime reserve) Vertic ustochrept. The results showed a significant response in pod yield with foliar and soil‐applied potassium as compared to the control treatment. Pod yields were significantly higher when basal and foliar applications were combined. The best results were achieved with foliar application of 1% KCl together with a basal fertilization with 50 kg K₂O ha^?1. Response to foliar‐applied K was higher in rainfed kharif crop than in irrigated rabi crop. Groundnut variety GAUG‐10 out yielded GAUG‐1. Foliar K application increased plant tissue concentration of K. Foliar fertilization with KCl and K₂SO₄ did not cause leaf burn. Potassium application improved the crop harvest index and grain quality parameters of boldness, protein and oil contents. Response to K in quality parameters of protein and oil contents of seed was more consistent with foliar applied K₂SO₄ . The results confirmed that the practice of foliar K nutrition when used as a supplement and not a substitute for standard soil fertilization, is beneficial for groundnut crop in Western India.     

跌落冲击下果实动态本构模型的构建与表征

基于果实非损伤条件下的跌落冲击动力学特征,提出表征其非线性黏弹性的本构模型与模型参数识别方法。该模型包括一个非线性弹簧元件、一个线性增强黏性元件。以苹果为研究对象,进行多个高度下的跌落冲击试验,获得其发生损伤的临界跌落高度;基于临界跌落高度下的冲击试验结果,建立其跌落冲击方程,进行跌落冲击响应的数值分析与模型参数识别。结果表明,理论结果与试验值很吻合,采用本模型结合试验结果能较准确地确定类似特性果实的动态流变特征参数。研究结果为果实动态流变特性分析以及模型表征提供了一种新方法,也为进一步表征果实的黏弹塑性损伤特征提供基础。     

Potassium fertilization of cotton on two high testing soils under two tillage systems

Potassium (K) fertilization of cotton (Gossypium hirsutum L.) has been a major research focus the last few years throughout the cotton belt. The objective of this field research, conducted from 1991 through 1994 on two high Mehlich I extractable K (EK) soils, was to evaluate broadcast and foliar applied K for conventional‐ (CT) and no‐tillage (NT) production. Main plot broadcast K rates were 0, 28, 56, and 112 kg K ha^‐1. Foliar sub‐plot treatments were a non‐foliar check, KNO₃, and Ca(NO₃)₂. The KNO₃ was applied four times per year at 4.1 kg K ha^‐1 application^‐1. Calcium nitrate was applied at 1.6 kg N ha^‐1 to equal the N applied in the KNO₃. Extractable K increased annually with broadcast K for both tillage systems and soils and was higher for NT than CT. Lint yields from CT of both soils were increased two of the eight site‐years while yields from NT were increased five of eight site‐years by broadcast K. Three of the NT site‐year yields plus four‐year mean yields of both soils were increased by applying 56 kg K ha^‐1, a rate higher than currently recommended for high EK soils. Yield responses to foliar fertilization were from added N rather than the K. Petiole K levels were sufficient so that extra K applied foliarly was not recommended for either soil or tillage system.     

Relationship of fertilizers on soil ph and fruit disorders of apples and pears

Abstract

In nutrition experiments of fruit‐growing areas of central Washington state, ‘Delicious’ and ‘Golden Delicious’ apple (Malus domestica) orchards and ‘d'Anjou’ pear (Pyrus communis L.) orchards with the lowest soil pH values had a high incidence of fruit disorders and the lowest concentration of leaf calcium (Ca). In contrast, orchards with the highest soil pH values had the highest concentration of soil Ca (meq 100 g^‐1) and the lowest incidence offrait disorders (bitter pit for apples and alfalfa greening, black end and cork spot for pears). Apple orchards with the highest soil pH values also had ‘Delicious’ and ‘Golden Delicious’ apples containing the highest concentration of Ca, but Ca concentrations in ‘d'Anjou’ pears were not significantly different among the different soil pH groups.     

Zinc–nitrogen interaction effect on wheat biofortification and nutrient use efficiency

Background : The enhancement of zinc (Zn) concentration in cereal crops without compromising yield is a global challenge with crucial health and food security implications. Aims : To achieve Zn biofortification in wheat through the appropriate management of fertilization with both Zn and nitrogen (N), due to the synergistic effect between them, using natural organic sources of Zn. Methods : We carried out a field experiment using a rainfed winter wheat (Triticum aestivum L.) crop fertilized with four Zn sources (Zn‐sulphate, Zn‐lignosulphonate, Zn‐amino acids and Zn‐gluconate) and three N application rates under semi‐arid conditions. Results : The strategy of increasing the N rate by 50% with respect to the recommended N rate (i.e., 120 kg N ha^?1) did not improve either wheat yield or grain Zn‐N concentration. The combined effect of applying natural organic Zn complexes and the recommended N rate tended to increase grain Zn concentrations (by an average of 14%), although this increase was significantly higher when Zn‐sulphate was applied (63%) due to its higher recommended Zn application rate. Natural organic Zn fertilizers achieved the highest grain yields, probably due to the enhancement of N uptake. The natural organic Zn fertilizers resulted in higher Zn utilization efficiency compared with the Zn‐sulphate fertilizer. Conclusions : In calcareous Zn‐deficient soils, our results suggest that Zn–N co‐fertilization involving Zn‐sulphate combined with the recommended N application rate would be advisable for obtaining grain Zn biofortification, while the highest yields can be obtained with the application of natural organic Zn fertilizers.     

The effect of selenium biofortification in alfalfa (Medicago sativa)

Abstract

Biofortification of agricultural productions is important to enhance the quality of the products. The aims of this research were to investigate the possibility of selenium biofortification of alfalfa in the calcareous and non-calcareous soils by applying fertilizers differently (control, soil application, inoculant of seleno-bacteria, foliar application, combined soil, and foliar applications of selenate). Morpho-physiological properties and nutritional responses, such as shoot dry weight, phosphorus, potassium, selenium, zinc, and iron concentration in the plant’s tissue were measured. In all parameters, the use of selenium sources in the calcareous soils had a significant effect compared with the control treatment. Among the methods and sources of selenium, foliar application of sodium selenate gave the best response. By the usage of this treatment, Se concentration was increased up to 112.5% and 182%, respectively, compared with the control treatment in non-calcareous and calcareous soil. According to the results, inoculant of selenium bacteria and sodium selenate are recommended as a natural way to selenium biofortification in alfalfa and to improve the quality of the produced forage. In addition, attention to the biological potential of the soil and the use of native soil bacteria is also recommended.     

Zinc and amino acids on wheat-soybean intercropping under no-till management

Abstract

Foliar fertilization with micronutrients and amino acids (AAs) has been used to increase the grain yield and quality of different crops. The aim of the present study was to evaluate the effects of Zn and AAs foliar application on physiological parameters, nutritional status, yield components and grain yield of wheat-soybean intercropping under a no-till management. We used a randomized block experimental design consisting of eight treatments and four replicates. The treatments were five Zn rates (0, 1, 2, 4 and 8?kg ha^?1) and 2?L ha^?1 of AAs and three additional treatments: a control (without the Zn or AA application), 2?kg ha^?1 Zn and 2?kg ha^?1 Zn + 1?L AA. The treatments were applied by spraying during the final elongation stage and at the beginning of pre-earing for the wheat and in growth stage V6 for the soybean for two crop years in a Typic Oxisol (860?g kg^?1 clay). Zinc foliar fertilization increased the wheat grain Zn concentrations. The Zn rates and AA foliar fertilization in soil with did not affect the physiological parameters, nutrient status or yield components. The AA application at the different concentrations tested changed the soybean grain yield and the leaf N concentration. The results suggest that Zn and amino acids application increases the grains Zn concentration in the wheat, being an important strategy to agronomic biofortification.     

A critical assessment of the suitability of phosphite as a source of phosphorus

Marketing of phosphite‐containing preparations for foliar application, together with recent reports of positive yield responses, has revived the question as to whether phosphite (HPO ) is a suitable P source for plants. Two experiments using zucchini (Cucurbita pepo L. convar. giromontina) have been conducted to evaluate the P‐nutritional effect of phosphite either provided via the substrate or as a foliar spray. Plants grown in a P‐deficient substrate were severely damaged when phosphite was applied as foliar fertiliser and more drastically when provided via the substrate. Growth of P‐deficient plants receiving phosphite as a foliar spray was impaired in a dose‐dependent manner after foliar P application (concentrations 0.0, 0.9, 2.7, and 4.5 g P L^–1), while foliar provision of phosphate improved plant growth and yield. In the youngest leaves of phosphite‐treated plants, which had developed after foliar spray, phosphite accumulated to considerable extent, reaching a similar concentration as phosphate at tissue level. These results confirm that P‐deficient plants are very sensitive to phosphite, which represents a nutritionally ineffective form of P. It should thus not be considered as a form of P suitable for fertiliser manufacture.     

Variability in Response to Zinc Application in 10 High-Yielding Wheat Varieties

A pot culture experiment was conducted to study the effect of zinc (Zn) on biofortification of 10 wheat (Triticum aestivum L.) varieties in the Zn-deficient soil of Lucknow. Treatments consisted of 0 and 20 mg Zn kg^?1 as a basal dose and 20 mg Zn kg^?1 basal dose with two foliar sprays of zinc sulfate (ZnSO₄) 0.5%. Foliar sprays of Zn were applied twice at the preflowering stage and 7 days after flowering. Results from the present study revealed that poor growth of plants grown in soil without Zn applications (0 mg Zn kg^?1) were improved by applications of Zn (20 mg Zn kg^?1) more when Zn was applied with two foliar sprays. Application of Zn (20 mg Zn kg^?1) with two foliar sprays also proved beneficial for maximizing Zn concentrations of grains and other plant parts. Wheat varieties NW 1076, K 3827, NW 2036, and UP 262 appeared highly responsive to the treatments.     

Mineral nutrition during establishment of golden delicious ‘smoothee’ apples on dwarfing rootstocks and interstems

This study was conducted to determine the influence of 4 interstems (EM.27 EMLA, Mark, M.9 EMLA, and EM.26 EMLA) and 8 rootstocks (EM.27 EMLA, Mark, M.9 EMLA, EM.26 EMLA, M.7A, MM. 106 EMLA, MM. 111 EMLA, and seedling) with and without interstems on foliar element concentrations [nitrogen (N,) phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), manganese (Mn), iron (Fe), zinc (Zn), boron (B)] of the Golden Delicious ‘Smoothee’ (Malus domestica, Borkh). The trees were planted in 1990 and the experiment was conducted until 1996. Soil pH was low (pH=5.9) before planting but liming raised the pH to 6.5 by the 4th year after planting. Soil P was adequate, K and Mg were high, and Ca was low based on local recommendations for apples. The year by year variation in foliar element concentrations was much higher than rootstock and interstem effects. Differences among interstems and rootstocks were important as foliar element concentrations approached those of deficiency or toxicity. In this study, K decreased to deficiency concentrations by the end of the experiment except for seedling rootstocks, which slightly increased. Foliar Ca was deficient for all interstems and rootstocks at the start of the experiment, but increased extensively for M.9 EMLA and EM.26 EMLA rootstocks across years. Foliar Mn increased to nearly toxic concentrations (300 μg g^‐1) in EM.27 EMLA and Mark rootstocks, whereas the other rootstocks did not. No deficiency or toxicity symptoms were noted for any elements during this study. These results indicate that a single range of foliar nutrient concentrations can be used as an aid for determining fertilization rates for the apple rootstocks and interstems used in this study. However, individual rootstocks vary in the rate at which they approach toxicity and deficiency concentrations, which needs to be known to prevent mineral nutritional related problems in commercial apple orchards.     

BORON UPTAKE AND DISTRIBUTION IN FIELD GROWN CITRUS TREES

In low fertility tropical soils, boron (B) deficiency impairs fruit production. However, little information is available on the efficiency of nutrient application and use by trees. Therefore, this work verified the effects of soil and foliar applications of boron in a commercial citrus orchard. An experiment was conducted with fertigated 4-year-old ‘Valencia’ sweet orange trees on ‘Swingle’ citrumelo rootstock. Boron (isotopically-enriched ¹⁰B) was supplied to trees once or twice in the growing season, either dripped in the soil or sprayed on the leaves. Trees were sampled at different periods and separated into different parts for total B contents and ¹⁰B/¹¹B isotope ratios analyses. Soil B applied via fertigation was more efficient than foliar application for the organs grown after the B fertilization. Recovery of labeled B by fruits was 21% for fertigation and 7% for foliar application. Residual effects of nutrient application in the grove were observed in the year after labeled fertilizer application, which greater proportions derived from the soil supply.     

Nitrogen fertilization effects on foliar nutrient dynamics and autumnal resorption in maidenhair tree (Gingko biloba L.)

We quantified seasonal foliar nutrient dynamics and autumnal N and P resorption of individuals of the deciduous gymnosperm Gingko biloba (maidenhair tree) growing in compacted, urban soils over two years following a single fertilization with 150 kg/ha/yr of N (and no P). Mean foliar nutrient concentrations were more similar to those reported for angiosperm trees than for other gymnosperms. During the autumn following fertilization, 46% and 48 of foliar N and P were resorbed, respectively; during the second year both increased significantly, to 68% of foliar N and 74% of foliar P. Absolute N resorption was also greater during the second year, whereas absolute P resorption did not differ significantly between years. During the second year, the level of N resorption rates was similar to that reported for Larix laricina (larch or tamarack), a needle‐leafed, deciduous gymnosperm, and greater than those reported for broad‐leafed deciduous angiosperms. The level of 1992 P resorption was similar to those reported for a wide range of gymnosperms and greater than those reported for most angiosperms. N fertilization allowed Gingko trees to maintain typical foliar N dynamics even during a persistent drought during which foliar P dynamics were significantly altered.     

Nutrient status of apple leaves not affected by three years of irrigation using partial rootzone drying

Partial rootzone drying (PRD) is a water‐saving irrigation technology that may affect apple (Malus domestica Borkh cv. Golden Delicious/Malling7)‐tree nutrition if applied for an extended period. The objective of this study was to test the hypothesis that long‐term application of PRD causes seasonal changes in macro‐ and micronutrients of apple leaves. The irrigation treatments were: (1) commercial irrigation as control (CI) and (2) PRD. After 3 years of evaluation, PRD irrigation had saved about 3240 m³ of water per hectare. Leaf xylem water potential was slightly lower in the PRD treatment than in CI. The seasonal concentration of macro‐ and micronutrients was comparable between treatments, although significant differences were found at times. The macronutrient concentrations were within the normal range in PRD apple leaves. All micronutrient concentrations were slightly above the normal range except for Zn, which was slightly below the normal range. No physiological disorders associated with plant nutrition were observed on leaves or fruits. Therefore, data suggest that PRD did not alter apple‐tree nutrition during the 3‐year trial. Thus, PRD may be feasible for apple production in Central Mexico. However, further studies need to be conducted in those regions where groundwater is the main water source for irrigation and rain is negligible, particularly during the growing season.     

Influence of mid‐summer boron sprays on boron content and quality indices of ‘delicious’ Apple 1

Boron (B) is required for optimal yield and quality of apple fruit (Malus domestica Borkh.) but may impair fruit quality if present in excessive amounts. A field experiment was conducted to examine the effects of a single mid‐July foliar B spray (0, 11.3, 22.6 g B/tree) on the B content and postharvest quality indices of 220‐gram ‘Starking Delicious’ apples. Fruit B concentration was positively related to B application rate and ranged from 9 to 55 mg/kg dry mass (1.3 to 7.7 mg/kg fresh mass). The relative B increases were greater in the core and inner cortex than in the outer cortex and skin, suggesting that some of the applied B entered the fruit through the tree vascular system. Increasing fruit B concentrations caused minor changes in fruit external color indices L and b and internal color index b but had no effect on firmness, soluble solids concentration, titratable acidity, starch index, external color index a, or internal color indices L and a. None of the effects were of horticultural significance. Most fruit quality indices were influenced by postharvest sampling time and reflected typical postharvest ripening patterns. The results suggest that ‘Delicious’ apple quality is relatively insensitive to high fruit B concentrations.     

Boron Fertilization and Evaluation of Four Soil Extractants: Russet Burbank Potato

Yield‐response correlations with old and improved soil extraction methods for boron (B) are needed. Russet Burbank potato (Solanum tuberosum L.) was grown with two, four, and six B treatments applied in 2004, 2005, and 2006, respectively. Zero and 1.1, 2.2, or 3.4 kg B ha^?1 soil and 0.22 or 0.28 kg B ha^?1 foliar treatments were applied. Boron fertilization did not significantly increase tuber yield or quality despite initially low hot‐water‐extractable B (0.34–0.50 mg kg^?1), although postseason B for unfertilized treatments increased (0.51–0.57 mg kg^?1). Soil‐applied B generally reflected B application relative to the untreated control and the low foliar rates in all three years for the four soil extractions utilized [hot water, pressurized hot water, diethylenetriaminepentaacetic acid (DTPA)–sorbitol, and Mehlich III]. Boron content of potato petiole did reflect application of B in 2 years, but tuber and peel tissues did not consistently reflect application of B.     

Foliar application of molybdenum in common bean. II. Nitrogenase and nitrate reductase activities in a soil of low fertility

Foliar application of molybdenum (Mo) at 40 g ha^‐1 25 days after plant emergence greatly enhanced nitrogenase and nitrate reductase activities of common bean (Phaseolus vulgaris L.), resulting in an increase in total nitrogen (N) accumulation in shoots. Application of 20 kg N ha^‐1 as ammonium sulfate [(NH₄)₂SO₄] at sowing decreased nodulation and nitrogenase activity. Rhizobium inoculation did not affect nitrogenase activity which demonstrated that Mo application increased the efficiency of native Rhizobia strains. Nitrogen amendment, either at planting (20 kg N ha^‐1) or as a side dressing (30 kg N ha^‐1) 25 days after plant emergence, did not affect the foliar nitrate reductase activity. Molybdenum foliar spray as ammonium molybdate [(NH₄)₆Mo₇O₂₄2H₂O] and N applied as a side dressing increased equally the total amount of N in the pods. A 10% increase in the seed N concentration was obtained with foliar application of Mo, while N applied as a side dressing had no effect on seed N concentration. An average increase of 41% in N export to the seeds was obtained by either Mo or N as side dressing. Nitrogen applied at sowing or Rhizobia inoculation had no effect on the characteristics evaluated 74 days after plant emergence. Plants that received either Mo as foliar spray or as side dressed N had similar yields. This demonstrated that, in certain soils, N fertilization may be replaced by a small amount of Mo as a foliar application.