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1.
Background : Since recently, the traditional rice–wheat rotation systems in Nepal are subject to drastic changes. Progressing urbanisation and shifting consumer preferences drive a replacement of wheat by high‐value vegetables during the cold dry season, particularly in the peri‐urban fringes, while emerging water shortages prevent permanent soil flooding during the monsoon season, leading to partial substitution of lowland rice by less water‐consuming upland crops. Associated changes in soil aeration status affect soil nutrient availability while particularly vegetables enhance the demand for the critically limiting micronutrients boron (B) and zinc (Zn). Aim : In both rice‐ (anaerobic) and maize‐based (aerobic) systems we assessed the differential response of traditional winter wheat in comparison to cauliflower and tomato to applied B and Zn fertilizers. Methods : Experiments were conducted (1) in a pot trial with two contrasting soil types (Acrisol vs. Fluvisol) and (2) in field validation trials at two contrasting sites (representing lowland vs. mid‐hills) in Nepal. Results : The on‐going shift from flooded rice to aerobic maize during the wet season negatively affected dry matter accumulation and grain yield of the dry season wheat, but not of cauliflower and tomato. While Zn application tended to increase wheat yields under field conditions, B application induced no significant effect, irrespective of the soil or production site. However, low to moderate applications of B (2.0–4.4 kg ha?1) and Zn (3.3–4.4 kg ha?1) nearly doubled biomass accumulation and nutrient uptake of vegetables and increased the economic yields of cauliflower and tomato between 8 and > 100%. These responses were generally more pronounced in the Fluvisol than the Acrisol. While overall yields of wheat and temperate vegetables were higher in the cool mid‐hills the relative yield responses to applied B were more pronounced in the lowland than the mid‐hill sites. On average, the partial factor productivities of applied fertilizer were low to moderate in wheat, with 1 and 8 € increase in net revenue per € of investment in B and Zn, respectively. In the vegetables, this partial factor productivity increased to about 4 € €?1 investment with Zn, and reached about 43 € €?1 investment in B, irrespective of the production site. Conclusions : While the application of Zn fertilizers can moderately improve the performance of traditional rice–wheat rotations, B and to a lesser extent Zn application become essential and highly profitable when shifting towards vegetable cropping. The demand for B and Zn fertilizers is foreseen to dramatically increase with progressing urbanisation and the associated shifts in production systems of Nepal. 相似文献
2.
Abdul Rashid Hari Ram Chun-Qin Zou Benjavan Rerkasem Aildson P. Duarte Simunji Simunji Atilla Yazici Shiwei Guo Muhammad Rizwan Rajinder S. Bal Zhaohui Wang Sudeep S. Malik Nattinee Phattarakul Rogerio Soares de Freitas Obed Lungu Vera L. N. P. Barros Ismail Cakmak 《植物养料与土壤学杂志》2019,182(5):791-804
Seeds enriched with zinc (Zn) are ususally associated with better germination, more vigorous seedlings and higher yields. However, agronomic benefits of high‐Zn seeds were not studied under diverse agro‐climatic field conditions. This study investigated effects of low‐Zn and high‐Zn seeds (biofortified by foliar Zn fertilization of maternal plants under field conditions) of wheat (Tritcum aestivum L.), rice (Oryza sativa L.), and common bean (Phaseolus vulgaris L.) on seedling density, grain yield and grain Zn concentration in 31 field locations over two years in six countries. Experimental treatments were: (1) low‐Zn seeds and no soil Zn fertilization (control treatment), (2) low‐Zn seeds + soil Zn fertilization, and (3) Zn‐biofortified seeds and no soil Zn fertilization. The wheat experiments were established in China, India, Pakistan, and Zambia, the rice experiments in China, India and Thailand, and the common bean experiment in Brazil. When compared to the control treatment, soil Zn fertilization increased wheat grain yield in all six locations in India, two locations in Pakistan and one location in China. Zinc‐biofortified seeds also increased wheat grain yield in all four locations in Pakistan and four locations in India compared to the control treatment. Across all countries over 2 years, Zn‐biofortified wheat seeds increased plant population by 26.8% and grain yield by 5.37%. In rice, soil Zn fertilization increased paddy yield in all four locations in India and one location in Thailand. Across all countries, paddy yield increase was 8.2% by soil Zn fertilization and 5.3% by Zn‐biofortified seeds when compared to the control treatment. In common bean, soil Zn application as well as Zn‐biofortified seed increased grain yield in one location in Brazil. Effects of soil Zn fertilization and high‐Zn seed on grain Zn density were generally low. This study, at 31 field locations in six countries over two years, revealed that the seeds biofortfied with Zn enhanced crop productivity at many locations with different soil and environmental conditions. As high‐Zn grains are a by‐product of Zn biofortification, use of Zn‐enriched grains as seed in the next cropping season can contribute to enhance crop productivity in a cost‐effective manner. 相似文献
3.
Emin Bülent Erenolu 《植物养料与土壤学杂志》2019,182(3):496-501
The present study aimed to test the contribution of the iron (Fe) deficiency‐induced uptake system to zinc (Zn) and copper (Cu) uptake by using bread wheat (Triticum aestivum cv. Bezostaja). For this purpose, two different uptake experiments, long‐term and short‐term, were set up in a nutrient solution culture under controlled growth chamber conditions. For the long‐term experiment, wheat cv. plants were grown with different concentrations of Fe or Zn. Results show that there was an uptake system induced under Fe‐limiting conditions which also contributed to Zn and Cu uptake. However, the Zn deficiency‐induced uptake mechanism affected neither Fe nor Cu uptake by wheat. Short‐term uptake experiments indicate that Fe deficiency‐induced Zn2+ uptake was more enhanced than the absorption of Zn‐phytosiderophore (PS) complexes. In addition, the Fe‐deficient plants absorbed more Zn in comparison to those plants supplied with sufficient Fe. Similar tendencies in Zn uptake under Fe deficiency in both short‐ and long‐term experiments suggest that there may be a specific Fe uptake system induced under Fe‐limiting conditions for non‐chelated metals in bread wheat. Moreover, this system also contributes to the transport of inorganic forms of some other metals, such as Zn and Cu. Although evidence is still needed involving the use of molecular biological techniques, it is hypothesized that IRT‐like proteins are responsible for this uptake system. Moreover, the release of Fe deficiency‐induced phytosiderophores and uptake of Fe(III)‐phytosiderophore complexes may not be the only mechanisms involved in the adaptation of wheat to Fe‐limiting conditions. 相似文献
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《植物养料与土壤学杂志》2017,180(2):231-240
Selenium (Se) is an essential micronutrient for humans, animals, and certain lower plants, but at higher concentrations Se becomes toxic to organisms. The boundary between the Se beneficial effect and its toxicity is narrow and depends on its chemical form, applied concentration, and other environmentally regulating factors. Due to the potential risk of toxicity in higher concentration, the aim of this study was to estimate the impact of increased concentrations of different forms of Se on the response of the wheat–soil–earthworm system. Soil, earthworms, and wheat grains were exposed to the Se in form of selenite and selenate in concentrations of 0.01, 0.1, and 1 mg kg−1. As an indicator of oxidative stress in wheat, lipid peroxidation levels (LPO) and total H2O2 content were determined, while antioxidative response was determined by catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR) activities. The biomarker responses in earthworms were determined by acetylcholinesterase (AChE), carboxylesterase (CES), and antioxidative enzymes (CAT and glutathione S‐transferase) activities. Selenite and selenate increased Se content in the wheat and earthworms, while selenate application was more efficient, indicating higher bioaccumulation of this Se form. Both Se forms did not cause significant changes in the LPO level and H2O2 content, while GPX activities were elevated in all treatments, suggesting that oxidative stress was not induced in wheat. In earthworms, Se significantly reduced activities of AChE and CAT at some concentrations, while CES activity was increased at all concentrations applied. This study showed significant impact of Se on measured biochemical responses in wheat and earthworms, indicating the disruption of homeostasis. Obtained results can serve as basis for further studies on Se effects and will help in including different aspects necessary for understanding of Se impact on different components of soil ecosystems. 相似文献
5.
Despite numerous studies on phytosiderophores (PS) there is still an open question whether nickel (Ni) deficiency induces release of PS from graminaceous plant roots. Seedlings of two wheat cultivars (Triticum aestivum L. cvs. Rushan and Kavir) and a triticale cultivar (X. triticosecale) were grown in Ni‐free nutrient solution (Ni‐deficient, Ni–) and with 10 µM NiSO4 (Ni‐sufficient, Ni+, control). Root exudates were collected weekly for 4 weeks and the amount of PS in the root exudates was measured. The response to Ni deficiency on the release of PS differed between species. Roots of Rushan and triticale exuded higher PS in response to Ni‐deficient conditions. Nickel deficiency significantly enhanced shoot Fe and Zn concentrations in wheat, while it decreased shoot Fe and Zn concentrations in triticale. In Kavir, PS exudation was decreased by Ni deficiency at weeks 3 and 4 and the reduced release of PS from roots of Kavir was accompanied by lower concentrations of Fe and Zn in plant roots but higher Fe and Zn concentrations in shoot tissue. The PS release by Kavir was triggered by a Ni‐induced Zn deficiency particularly in the shoots. According to the results, it is suggested that in the studies concerning the phytosiderophore release under Ni deficiency, special attention should be given to different responses among and within cereals and to the plant Zn or Fe nutritional status. 相似文献
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Partha Talukdar Sue E. Hartley Anthony J. Travis Adam H. Price Gareth J. Norton 《植物养料与土壤学杂志》2019,182(2):265-276
Silicon in rice (Oryza sativa L.) has been demonstrated to be involved in resistance to lodging, drought, and salinity, and also enhances resistance to pests and diseases. The aim of this study was to determine the range of silicon concentration in a set of rice (Oryza sativa L.) accessions, and to determine if the natural variation of shoot silicon is linked to the previously identified silicon transporters (Lsi genes). Silicon concentration was determined in 50 field‐grown accessions, representing all sub‐populations of rice, with all accessions being genotyped with 700K SNPs. SNPs within 10 kb of the Lsi genes were examined to determine if any were significantly linked with the phenotypic variation. An XRF method of silicon determination compared favourably with digestion and colorimetric analysis. There were significant genotypic differences in shoot silicon ranging from 16.5 to 42.4 mg g?1 of plant dry weight, but there was no significant difference between the rice sub‐populations. Plants with different alleles for SNPs representing Lsi2 and Lsi3 were significantly different for shoot silicon concentration. Shoot silicon correlated negatively with grain arsenic in the tropical and temperate japonica sub‐population, suggesting that accessions with high shoot silicon have reduced grain arsenic. This study indicates that alleles for Lsi genes are excellent candidate genes for further study to explain the natural variation of shoot silicon in rice. 相似文献
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Minh Luan Nguyen Johann Glaes Stijn Spaepen Bernard Bodson Patrick du Jardin Pierre Delaplace 《植物养料与土壤学杂志》2019,182(3):325-334
Bacillus velezensis strains, belonging to plant growth‐promoting rhizobacteria (PGPR), are increasingly used as microbial biostimulant. However, their field application to winter wheat under temperate climate remains poorly documented. Therefore, three B. velezensis strains IT45, FZB24 and FZB42 were tested for their efficacy under these conditions. Two biological interaction systems were firstly developed under gnotobiotic and greenhouse conditions combined with sterile or non‐sterile soil, respectively, and finally assayed in the field during two years coupled with different N fertilization rates. Under gnotobiotic conditions, all three strains significantly increased root growth of 14 d‐old spring and winter wheat seedlings. In the greenhouse using non‐sterile soil, only FZB24 significantly increased root biomass of spring wheat (+31%). The three strains were able to improve nutrient uptake of the spring wheat grown in the greenhouse, particularly for the micronutrients Fe, Mn, Zn, and Cu, but the observed increases in nutrient uptake were dependent on the organs and the elements. The root biomass increases in inoculated plants coincided with lowered nutrient concentrations of P and K. In 2014, under field conditions and absence of any N fertilizer supply, FZB24 significantly increased grain yields by 983 kg ha?1, or 14.9%, in relation to non‐inoculated controls. The three strains in the 2015 field trial failed to confirm the previous positive results, likely due to the low temperatures occurring during and after inoculations. The Zeleny sedimentation value, indicative of flour quality, was unaffected by the inoculants. The results are discussed in the perspective of bacterial application to wheat under temperate agricultural practices. 相似文献
9.
Koji Yamane Ranee C. Mabesa-Telosa Ryosuke Tajima Nino P. M. C. Banayo Yoichiro Kato 《植物养料与土壤学杂志》2019,182(1):28-30
Simple tools for diagnosing crop nutritional status are in great demand by agricultural extension staff, particularly in low‐fertility environments. We developed the first practical method for in situ diagnosis of phosphorus (P) nutrition in field‐grown cereal crops by using a handheld colorimeter. The concentration of P in extracts from fresh leaves of lowland rice grown under various P availabilities was closely correlated with that of oven‐dried leaves measured with the standard molybdenum‐blue method in the laboratory, over a range of values from 0.319 to 1.420 mg P g ?1 DW (r = 0.885**, n = 14). It takes only 3–5 min to estimate the P concentration of plant samples using the new technique. Although not as accurate as the laboratory method, the new method can easily detect P deficiency of rice in the field (and possibly other cereal crops) without requiring costly, off‐site equipment. Thus, extension staff and agronomists can easily adopt this technique. 相似文献
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It was hypothesized that the application of eucalyptus biochar enhances nutrient use efficiencies of simultaneously supplied fertilizer, as well as provides additional nutrients (i.e., Ca, P, and K), to support crop performance and residual effects on subsequent crops in a degraded sandy soil. To test this hypothesis, we conducted an on‐farm field experiment in the Khon Kaen province of Northeastern Thailand to assess the effects of different application rates of eucalyptus biochar in combination with mineral fertilizers to upland rice and a succeeding crop of sugarcane on a sandy soil. The field experiment consisted of three treatments: (1) no biochar; (2) 3.1 Mg ha?1 biochar (10.4 kg N ha?1, 3.1 kg P ha?1, 11.0 kg K ha?1, and 17.7 kg Ca ha?1); (3) 6.2 Mg ha?1 biochar (20.8 kg N ha?1, 6.2 kg P ha?1, 22.0 kg K ha?1, and 35.4 kg Ca ha?1). All treatments received the same recommended fertilizer rate (32 kg N ha?1, 14 kg P ha?1, and 16 kg K ha?1 for upland rice; 119 kg N ha?1, 21 kg P ha?1, and 39 kg K ha?1 for sugarcane). At crop harvests, yield and nutrient contents and nitrogen (N) use efficiency were determined, and soil chemical properties and pH0 monitored. The eucalyptus biochar material increased soil Ca availability (117 ± 28 and 116 ± 7 mg kg?1 with 3.1 and 6.2 Mg ha?1 biochar application, respectively) compared to 71 ± 13 mg kg?1 without biochar application, thus promoting Ca uptake and total plant biomass in upland rice. Moreover, the higher rate of eucalyptus biochar improved CEC, organic matter, available P, and exchangeable K at succeeding sugarcane harvest. Additionally, 6.2 Mg ha?1 biochar significantly increased sugarcane yield (41%) and N uptake (70%), thus enhancing N use efficiency (118%) by higher P (96%) and K (128%) uptake, although the sugar content was not increased. Hence, the application rate of 6.2 Mg ha?1 eucalyptus biochar could become a potential practice to enhance not only the nutrient status of crops and soils, but also crop productivity within an upland rice–sugarcane rotation system established on tropical low fertility sandy soils. 相似文献
12.
《植物养料与土壤学杂志》2017,180(6):739-747
Crop productivity in future may be limited due to water scarcity. However, foliar spray of plant growth promoters may boost crop production even in adverse environments. In the present study, foliar application of one natural (moringa leaf extract, 3% MLE) and four synthetic (Polydol, Multisol, Classic, and Asahi Star) were applied at tillering, jointing, booting, and heading growth stages of wheat (Triticum aestivum L.) during severe, moderate, and light drought and well‐watered condition. No spray and water spray were taken as controls. Results showed significant reduction in growth parameters such as total dry matter production, mean crop growth rate, net assimilation rate, leaf area index, and duration due to drought employed at various phenophases of wheat. However, improvement in these parameters was observed after foliar application of growth promoters, whereas interactive effects between factors were found non‐significant. The activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were more accelerated under drought treatments from exogenously supplied growth promoters. Foliar application of promoters significantly alleviated drought‐induced reduction of yield and related traits. Grain weight (15%) and grain yield (27%) were improved due to exogenously applied MLE under moderate drought stress treatments relative to controls. Furthermore, 16% higher grain yield and 17% saving of irrigation water over fully irrigated and without promoter treatment (farmers' practice) was recorded from foliar‐applied MLE under skipped irrigation at jointing. In conclusion, foliar‐applied MLE may ameliorate drought‐induced deleterious effects by enhancing antioxidant activities under drought stress. 相似文献
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《植物养料与土壤学杂志》2017,180(4):474-481
Potassium (K) deficiency reduces photosynthesis and biomass production of crop plants and also renders them vulnerable to drought stress, whereas elevated carbon dioxide (CO2) has a positive effect on photosynthesis and yield and ameliorates the adverse effects of drought stress. This study aimed to characterize the physiological responses of wheat (Triticum aestivum L.) stressed with K deficiency under elevated CO2 and drought conditions. Increased biomass production caused by elevated CO2 as a consequence of increased photosynthesis and water use efficiency was absent in young K‐deficient wheat plants. Shoot K concentration was negatively affected by elevated CO2 particularly under K‐deficient conditions, whereas K content per plant was greatest in plants supplied with adequate K and adequate water. Specific leaf weight was increased as a consequence of carbohydrate accumulation in the source leaves of K‐deficient plants particularly under elevated CO2 and drought stress. Potassium deficiency clearly impeded the impact of elevated CO2 in both well watered as well as drought‐stressed plants. Adequate K fertilization is a prerequisite for efficient harvesting of atmospheric CO2 through increased photosynthesis, decreased transpiration, and increased biomass production under changing atmospheric CO2 and soil moisture conditions. 相似文献
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《植物养料与土壤学杂志》2017,180(3):316-325
Increased application of nitrogen fertilizers has significantly raised grain yield and protein concentration in wheat. However, only 30–50% of applied fertilizer nitrogen are usually utilized by the plant. In this study, four soft red winter wheat genotypes (Triticum aestivum L., IL07‐4415, MD05W10208‐11‐8, OH06‐150‐57 and Sisson) were grown under three different nitrogen regimes (high, medium, and low) in a greenhouse, and grain yield, grain protein concentration, nitrogen use efficiency (NUE) and their associated traits were evaluated. Among the four genotypes, a high‐yielding cultivar, Sisson, exhibited superior performance in terms of grain weight plant−1 and NUE for yield (NUEY) at low nitrogen due to maintained grain number spike−1 and harvest index. Significant yield losses due to nitrogen limitation were attributable to reduced spike number plant−1 and grain number spike−1 in the other genotypes. Interestingly, a linear relationship between NUEY and NUE for grain protein (NUEP) was detected at high (R 2 = 0.67) and low (R 2 = 0.42) nitrogen; both of these traits were positively correlated with grain number spike−1, 1000‐seed weight, and harvest index under nitrogen‐limited conditions (R 2 = 0.35–0.48). These results suggest that simultaneous improvement of NUEY and NUEP could be achieved through the selection of the three yield components (grain number spike−1, 1000‐seed weight, and harvest index) at low nitrogen. 相似文献
16.
Crop nitrogen (N) uptake depends on the root absorption area and the soil N availability which are closely related to the soil water status. With the increasing water shortages in the North China Plain, supplemental irrigation (SI) to winter wheat is a promising technique. To clarify the relationships between water and nitrogen use, four SI regimes in Tritcum aestivum L. cv. Jimai 22 were set up: no‐irrigation after emergence (T1), SI at jointing and anthesis (T2), SI at sowing, jointing and anthesis (T3), and SI at pre‐wintering, jointing and anthesis (T4). The results indicate that T2 had higher root length density (RLD) and root surface area density (RAD) in the 0–20, 60–80, and 80–100 cm soil layers, as well as higher post‐anthesis N uptake from soil by 23–26% in 2012–2013 and 162–177% in 2013–2014, compared to T3 and T4. The grain yield under T2 was lower than T3 but was not significantly different from T4, whereas its water use efficiency (WUE) was higher relative to both T3 and T4. There were no significant differences among T2, T3, and T4 in N use efficiency (NUE). The N uptake after jointing and WUE were positively correlated with the RLD and RAD in the 0–20 cm soil layer. The NUE was positively correlated with the RLD and RAD in the 20–40 cm soil layer. These results indicate that timely SI at jointing and anthesis was dependent on a suitable water supply at sowing, which increased the soil water content in the upper soil layer after jointing and improved the absorption area of the roots in both the deep and surface soil layers; this further improved the post‐anthesis N uptake from the soil and the WUE. This approach can be a valuable way to maintain high grain yields and NUE in winter wheat while using less irrigation and achieving higher WUE in the North China Plain. 相似文献
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A field experiment was conducted to study the effects of boron (B), molybdenum (Mo), zinc (Zn) and their interactions on seed yield and yield formation of rapeseed (Brassica napus L. var. Huashuang 4). Application of B fertilizer to a sandy soil increased the seed yield by 46.1% compared to the control and also created a considerably higher seed yield than the two treatments solely applying Mo and Zn fertilizers, which suggested that B was a main constraint for the seed yield of Huashuang 4 in this experiment. The effect of B fertilizer on the seed yield was attributed to an increase in the number of seeds per silique and siliques per plant. The combined application of B with Mo or Zn resulted in higher seed yield than the application of B, Mo or Zn alone, and the seed yield of the B+Mo+Zn treatment was the highest in all treatments, 68.1% above the control. Dry matter accumulation of seed followed a typical S-shaped curve and it was higher in plants supplied with B than in plants without B. A small but significant increase in the seed oil content and an improvement in the oil quality were also observed in all treatments compared with the control. These results suggested that optimal micronutrient application could provide both yield and quality advantages for rapeseed in poor soil. 相似文献
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Arthrobacter ureafaciens K10, with multiple functions, such as feather degradation, phosphate solubilization, and IAA formation, and Streptomyces sp. CP3, with pitaya cladode‐degrading ability, were co‐inoculated in compost composed of pitaya‐pruning waste (PPW), spent mushroom waste (SMW), and duck feathers (DF) in order to produce a compost called Sapf‐M. Another compost, called Sapf, was manufactured via the same composting procedure but without inoculation. The two compost products were slightly acidic and had a bulk density less than 0.25 g cm?3, a water‐holding capacity (WHC) greater than 85%, a C : N ratio of nearly 10, and a germination index higher than 85%. Higher IAA concentration, EC, phosphate‐solubilizing bacterial numbers, actinobacterial numbers, and water‐soluble nutrients, such as ammonium, magnesium, copper, manganese, and iron, were found in Sapf‐M compared to Sapf. Five different culture media were prepared: peat mixed with Sapf at a 2 : 1 volume ratio (P‐Sapf), peat mixed with Sapf‐M at a 2 : 1 volume ratio (P‐Sapf‐M), Sapf, Sapf‐M, and peat. Each of these was analyzed for its ability to produce cherry tomatoes without fertilization. Higher WHC and water‐soluble ammonium were found in P‐Sapf‐M than in P‐Sapf. Cherry tomato cultured in P‐Sapf‐M showed the highest fruit number and yielded 1.04 kg per plant. Fruit firmness, splitting rate, BER incidence, TSS/acidity, and nutrition were all analyzed. After eradication, the mineral concentrations of roots and culture media were also determined. The results of this study show that the bio‐inoculated Sapf‐M had longer‐lasting fertility and greater plant growth‐promoting abilities than the Sapf when used as the growing medium for cherry tomato production without further fertilization. 相似文献
20.
Research often needs to determine iron (Fe) concentrations in plant tissue samples. Current established methods depend on equipment and often require skilled laboratory staff, large sample sizes, and are relatively slow and expensive. We propose an efficient and fast method for measuring Fe concentrations of small rice samples via a microplate reader using sodium dithionite (SDT) as reducing agent and dipyridyl (DPD) as coloring agent. The results show that the method yields results comparable to ICP‐OES measurements which were used as standard method. Different concentrations of the chemicals used were tested for extraction, reducing power, and coloring efficiency, to optimize the method for the range of concentrations to be expected in rice under toxic Fe conditions. Best results were obtained using 500 mM SDT and 10 mM DPD, a sample size of 0.01g dry weight, and Fast Prep as extraction method. A linear calibration curve was obtained for 0 to 100 mg kg?1 iron within the measured samples. The method proposed here was successfully applied to measure total Fe concentration in oven‐dried, milled plant samples. Applicability of the method for tissues other than rice and suboptimal extraction methods are discussed. 相似文献