The ecology of Sphyraena barracuda (Osteichthyes: Perciformes) in the Kosi system with notes on the Sphyraenidae of other Natal estuaries

The ecology of Sphyraena barracuda was studied in the Kosi estuary system of Natal. Fry enter from the sea at a length of 20 mm and live in shallow marginal weed-beds until a length of 80 mm when they move to fringing Phragmites reed-beds. After reaching 300 mm they move to open water areas. No specimens longer than 500 mm were recorded and none were mature. Growth is rapid, they attain 180 mm in the first six months and after two years measure 400-500 mm. They leave the estuary after about two years. Their significance in the estuarine fish community is due to their predation, at all sizes, on a variety of estuarine-dependent juvenile fishes (e.g. Rhabdosargus spp., Mugilidae) and adults of estuarine species (e.g. Ambassis spp.). S. barracuda was recorded at salinities between 4°/oo and 35°/oo, only in very clear water and at temperatures from 14,4 °C to 37 °C. Sphyraena bleekeri and S. qenie, which are also clear-water species, were found at Kosi in salinities from 0,5 to 35%o. They are also piscivorous on a range of small teleosts. Sphyraena iello is the most widespread barracuda in Natal estuaries, but only juveniles were recorded. This species is tolerant of a wide range of turbidities and salinities which enables it to live under most estuarine conditions. The distribution, ecology and interspecific interactions among Sphyraenidae in Natal estuaries are discussed in relation to their feeding biology, habitat preferenda and physical tolerances.     

Larval development of Sesarma catenate Ortmann (Brachyura,Grapsidae, Sesarminae) reared in the laboratory

Sesarma catenata larvae were raised in the laboratory and the development stages described in detail. Larval development comprises four zoea and one megalopa stage. Morphological features of the first zoea and megalopa stages of Sesarma catenata are compared with those of other Sesarminae species described in the literature. The genera Sesarma, Metasesarma, and Bresedium appear to be closely related taxa within the subfamily. The diagnostic value of some features of larval morphology is briefly discussed.     

Species identification,distribution and abundance of Gerreidae (Teleostei) Bleeker,1859 in the estuaries of Natal

Comparison of published meristic and taxonomic data with measurements taken from specimens collected from Natal estuaries have provided information for an identification key to the Gerres of southern Africa. Body markings were different on each species of fry (< 40 mm S.L.) as well as immatures and adults (> 40 mm S.L.). A comparison of otoliths from the different species showed that they can be used to Identify specimens from a standard length of 20 mm. Turbidity may be a factor limiting the distribution of the genus in estuaries. The known distribution of Gerres species in the estuaries of southern Africa is given. The species of Gerres which is dominant in a particular locality depends on the salinity.     

The ecology of two degraded estuaries on the north coast of Natal,South Africa

The biota of the Tongati and Mdloti estuaries were studied in relation to the environment and human interference. Results are compared with those obtained from a relatively undamaged estuary. The Tongati receives treated sewage effluent, is rarely closed from the sea, has low salinities and low oxygen tensions, but is rich in phosphorus and nitrogen. Energy values of benthic floe from Tongati were high and large quantities of water hyacinth (Eichomia crassipes) occurred. The zooplankton and zoobenthos were impoverished and dominated by freshwater species. The roots of Eichomia provided a habitat for many invertebrates, chiefly insect larvae and the crab Varuna iitterata. The fish fauna was poor and most species were confined to the lagoon near the mouth. Iliophagous species, mainly Mugilidae, were dominant. The Mdloti, frequently closed from the sea but often artificially opened, exhibited typical estuarine salinity patterns, was well oxygenated but relatively poor in phosphorus and nitrogen. Primary production and energy values of benthic floe were low. Zooplankton and zoobenthos were impoverished. The fish fauna, similar to that of Tongati, was dominated by Mugilidae.

The food chain from benthic floe to iliophagous fish remains viable in these degraded estuaries.     

Temperature and salinity tolerance of adult hermit crabs,Diogenes brevirostris,Stimpson (Crustacea: Decapoda: Anomura)

Diogenes brevirostris was found to be tolerant to low salinities (0,5 - 4,0 %) at a wide range of temperatures (12 to 27°C). Tolerance to temperature and salinity were inversely related. At low salinities (0,5 and 1,0 %) the rate of mortality increased with temperature throughout the range, whereas at higher salinities (1,5 and 4,0 0/00) temperature had no effect on mortality. These results are discussed in relation to the distribution of D. brevirostris in estuaries. It appears that temperature and salinity tolerance are not solely responsible for limiting distribution of these crabs to estuary mouths.     

Distribution and Tidal Rhythmicity of a Littoral Amphipod

The larval stages of the red mangrove crab Sesarma meinerti de Man were reared in the laboratory. Larval development consists of five zoeal stages and one megalopa. Zoeal development lasts an average of 25 days at 25°C. The external morphology of larvae is described in detail and their relationship with larvae of congeneric species is briefly discussed.     

Energy Metabolism of the Cattle Egret

Ages were estimated for the tropical sparid, Acanthopagrus berda, caught in northern KwaZulu-Natal estuaries. Whole otoliths were used in the age determination. Age estimates were validated by marginal increment analysis and oxytetracycline labelling, which indicated that opaque deposition occurs primarily from September to November each year. The reproducibility of age estimates was described by a coefficient of variation of 10%. The von Bertalanffy growth curve was found to best describe the growth of A. berda. The parameters of the von Bertalanffy growth curve indicated that A. berda in northern KwaZulu-Natal is a slowgrowing species, capable of reaching at least 16 years of age. Longevity of the species, coupled with sex change, late maturation and estuarine dependency, give cause for concern for the continued sustainable utilization of this species.     

The fish community of the berg river estuary and an assessment of the likely effects of reduced freshwater inflows

Data concerning the species composition, abundance and distribution of fishes inhabiting the Berg River estuary are presented and used to assess the value of the estuary to fish, and the likely eflects of reduced freshwater inflows. A total of 31 species was recorded, eight of which were classified as estuarine residents, 11 as marine estuarine-opportunists, six as marine stragglers and six were freshwater species. Liza richard- sonii, an opportunist, was the most abundant (54% N) and widely distributed species encountered during the winter and summer seine-net surveys. Three other species (all residents) contributed more than 10% of the total catch; Atherina breviceps and Caffrogobius nudiceps occurring most abundantly in the lower estuary, and Gilchristella aestuaria in the middle estuary. The low number of species compared with estuaries in other regions of the South African coast reflect a well-established marine biogeographical trend. The higher proportions ol resident species, entirely dependent species and partially dependent species suggest, however, that west coast estuaries may be more important to the fish that inhabit them than are estuaries in other regions. It is concluded that the fish community of the estuary is already suffering the effects of habitat degradation and that further reductions in freshwater inllows are not desirable.     

Description and ecology of larvae of Glossogobius callidus and Redigobius dewaali (Gobiidae) from temperate South African estuaries

This paper describes the morphology and ecology of the larvae and early juveniles of two common gobiids in warm temperate South African estuaries. The early developmental stages of Glossogobius callidus and Redigobius dewaali were collected during plankton surveys in seven permanently open and five intermittently open estuaries along southeastern South Africa. Larval G. callidus have a characteristic ventral row of pigment that extends from the cleithral symphysis along the ventral midline of the tail to the end of the caudal peduncle; notochord flexion takes place at the yolk-sac stage, between 3 and 4 mm BL. Larval R. dewaali are characterized by having two very large stellate melanophores on the ventral surface of the trunk and tail, and internal pigment between the snout and hindgut; notochord flexion takes place between 4 and 6 mm. Body lengths at settlement for G. callidus and R. dewaali are 13 mm and 16 mm, respectively. G. callidus and R. dewaali dominate the larval gobiid catch in warm temperate estuaries. Estuary type and freshwater input played a defining role in the occurrence of larvae of these, often sympatric, species in temperate estuaries. Larval G. callidus occurred in both permanently open and intermittently open estuaries, and were most abundant in mesohaline regions over the spring/summer period. By contrast, larval R. dewaali occurred exclusively in permanently open estuaries and were more prevalent in the fresh and oligohaline regions in autumn. Larvae of both species were more prevalent in freshwater-rich estuaries. Densities of G. callidus and R. dewaali peaked at 101 and 3829 larvae per 100 m³respectively. The usefulness of using the early stages of these gobiids as indicators of estuarine health based on their specific and often prolific estuarine occurrence is discussed.     

A survey of the fish fauna of Transkei estuaries Part Four: The Mntafufu and Mzamba River estuaries

The Mntafufu and Mzamba Rivers have small catchment areas (178 and 505 km², respectively) in the forested coastal belt of Transkei and have relatively small estuaries (5 and 2 km long, respectively). Although the annual rainfall is in excess of 1000 mm in this area, the mean annual runoff was less than 70 Mm³ in both cases. Serious floods were not observed during the survey period, while turbidity and sediment loads were usually very low. Salinities were usually high (x? = 30,0-38,0‰) in the bottom water and temperatures in the upper reaches were usually higher than in the lower reaches. The species composition, and seasonal and spatial abundance of the fish fauna of the two estuaries were determined by means of gill nets. A total of 1043 fish (385,8 kg) representing 42 species were caught in the Mntafufu estuary. The orangemouth glassnose Thryssa vitrirostris and V. cunnesius were the most abundant fish in the estuary. Numerically 42% of the catch (46% gravimetrically) belonged to the family Mugilidae (11 species) of which Mugil cephalus, Valamugil buchanani and V. cunnesius were the most important. A total of 389 fish (241,1 kg) were caught in the Mzamba estuary. Twenty-five species were obtained with Hilsa kelee dominating numerically. Seven species of mullet contributed 38,3% of the numbers or 54,2% of the fish biomass. The diamond mullet (Liza alata) and the flathead mullet (Mugil cephalus) were the most abundant in terms of biomass. The highest catches in the two estuaries were taken in the lower reaches. Mean number and biomass of fish taken per net was 20,5 fish and 7,2 kg, and 16,2 fish and 10,1 kg in the Mntafufu and Mzamba estuaries, respectively.     

Marine connectivity and fish length frequencies of selected species in two adjacent temporarily open/closed estuaries in the Eastern Cape Province,South Africa

The effects of timing, frequency and duration of mouth opening and marine overwash events on fish assemblages in two, adjacent temporarily open/closed estuaries were investigated. Sampling was conducted biannually over two years during summer and winter, using selective sampling gear to target estuarine-associated marine species. During this period the East Kleinemonde Estuary opened more frequently than the West Kleinemonde Estuary and it also experienced a greater number of bar overwash events when larval recruitment can occur. The timing of mouth opening and the frequency of marine overwash events did not influence species richness of marine-spawning species but had a pronounced effect on abundances (catch-per-unit-effort) of species. The species composition of both estuaries was similar, but the catch-per-unit-effort in the East Kleinemonde Estuary was more than three times greater than that of the West Kleinemonde Estuary. The East Kleinemonde also supported a greater proportion of immature individuals of Rhabdosargus holubi and Monodactylus falciformis, and the length frequency distributions of both these species differed significantly between the two estuaries. The larger fish sizes in the West Kleinemonde Estuary were attributed to the prolonged mouth closure that resulted in the trapping of larger juveniles and mature fish within the estuary.     

Effects of Salinity on Yersinia ruckeri Infection of Rainbow Trout and Brown Trout

Abstract

Juvenile rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta acclimated to freshwater or salinities of 9.0‰ or less were exposed to Yersinia ruckeri, the bacterial pathogen that causes enteric redmouth disease (ERM). Both species of fish were kept in the same recirculating systems after bacterial exposure. Rainbow trout mortality was significantly (P < 0.05) different in each salinity: 96.5% in freshwater, 89.5% in water of 1.1‰ salinity, 81.3% in 3.0‰ salinity, and 75.0% in 9.0‰ salinity (model SE = 1.0). All deaths occurred between 3 and 12 d after exposure to Y. ruckeri. Only 2.3% of brown trout in all salinities died, and differences among treatments were not significant. For both fish species, Y. ruckeri was isolated from liver, spleen, and trunk kidney of fish dying during this experiment, and lesions of rainbow trout were consistent with ERM. Yersinia ruckeri was not isolated from brown trout surviving for 21 d after bacterial exposure but was isolated from 3 of 24 surviving rainbow trout; a polymerase chain reaction assay detected the DNA of Y. ruckeri in 3 additional rainbow trout survivors. Neither the lesions of fish with ERM nor the percentage of surviving fish subclinically infected with Y. ruckeri was affected by salinity. Bacterial growth in vitro was not affected by low (≤9.0‰) salinity; however, bacterial adhesion to polystyrene was significantly reduced as salinity increased. Although mortality caused by Y. ruckeri was significantly lower for rainbow trout in water with slightly increased salinity, none of the salinities tested was effective in preventing serious losses caused by this pathogen in recirculating systems.     

The effect of temperature and salinity on oxygen consumption in the brachyuran crab Cyclograpsus punctatus (Crustacea: Decapoda: Grapsidae)

The aquatic oxygen consumption of the estuarine brachyuran crab, Cyclograpsus punctatus, was investigated after a 24-hour acclimation period at different temperature (12.5, 20, 30°C) and salinity (9, 17.5, 35, and 44°) combinations . Salinity had no significant effect on oxygen consumption at 12.5 and 20°C in both large and small crabs. At 30°C and 44°, however, the rate of oxygen consumption declined in large crabs (from 0.233 to 0.176 mg O₂ g wwt/h) and increased in small crabs (0.300 to 0.469 mg O₂ g wwt/h). There was also a significant increase in the oxygen consumption (0.300 to 0.536 mg O₂ g wwt/h) of small crabs at the high temperature/low salinity combination. Temperature had a significant effect on the rate of oxygen consumption at all salinities, with Q₁₀ values ranging from 1.16 to 2.85. In all cases the mass-specific oxygen consumption rate of small crabs was higher than that of large crabs. The results suggest that the confinement of this species to the lower reaches of estuaries may, in part, be due to physiological constraints on juveniles and small individuals.     

Turbidity-induced changes in feeding strategies of fish in estuaries

The aim of this study was to determine the effect of turbidity on the feeding strategies of fish in estuaries. Three species representing different feeding guilds were selected for the investigation. These were Elops machnata (representative piscivore), Pomadasys commersonnii (a macrobenthivore) and Atherina breviceps (a planktivore). The stomach contents of these fish were examined from a clear and a turbid estuary and some experimental work was carried out on A breviceps to test the hypothesis that turbidity affects feeding behaviour. Turbidity was found to have no effect on size selection of prey, but feeding rate, particularly of visual predators, was reduced at higher turbidity levels. This was caused by a decrease in the reactive distance of the fish. It would appear that in order to optimize the aquisition of food under different turbidity conditions fishes have the ability to change their feeding strategies. Visual predators are more affected by turbidity than are macrobenthic feeders.     

Thermal and salinity effects on locomotor performance of Thoropa taophora tadpoles (Anura,Cycloramphidae)

It is well known that environmental temperature influences several biological functions of ectotherms, notably in amphibians. The high permeability of anuran skin, associated with the effect of elevated environmental temperature, potentiates the dehydration process and this combination may restrict locomotor performance. Thoropa taophora is an endemic species from the Atlantic Rainforest whose tadpoles are semiterrestrial and predominantly diurnal, and are found in rocky seashores where they are exposed to sea spray and high temperatures. In this study we investigated how temperature and salinity conditions affect the locomotor performance in Thoropa taophora tadpoles. We also assessed how different osmotic concentrations affect the activity of the metabolic pathways that support muscle function. We measured the sprint speed of tadpoles of various sizes at different temperatures and salinities in the field. We also measured the activity of the enzymes pyruvate kinase (PK), lactate dehydrogenase (LDH) and citrate synthase (CS) in different temperatures and osmotic concentrations, and calculated the thermal sensitivity and the activity constants for each osmolality. Our results showed that, in general, sprint speed decreased with increasing temperature and salinity. However, whereas the effect of increased salinity was similar in smaller and larger tadpoles, increased temperature had a higher negative impact on sprint speed of larger tadpoles, thus indicating low thermal sensitivity of small tadpoles. PK and LDH thermal sensitivities and LDH constant of activity decreased as the osmolality increased. In conclusion, the locomotor capacity of tadpoles was decreased by temperature and salinity, which may be related to a decrease in anaerobic metabolism both in terms of sensitivity and total energy turnover through enzymatic activity. We discuss the ecological consequences, including the potential impacts on predator escape behavior promoted by changes in metabolism and locomotor performance in an early stage of development of this species.     

Disentangling the identities and distribution patterns of the introduced beachfleas Orchestia gammarellus and Platorchestia platensis (Crustacea: Amphipoda: Talitridae) in South Africa

Two introduced beachfleas, Orchestia gammarellus and Platorchestia platensis, have been recorded from South Africa, but have been misidentified by some earlier researchers and, prior to this study, each was also known from just a few, scattered observations, such that their true distributions remained obscure. We illustrate both species to clarify the features that identify and distinguish them and determine their true distributions by re-examining historical samples and by collecting new material from estuaries and lagoons along the west and south coasts of South Africa. Adult males of O. gammarellus have slender antennae and characteristic expanded, flattened, oar-like distal segments on pereopod 7. Adult males of P. platensis have the peduncle of antenna 2 strongly swollen and have bulbous, but not flattened, distal segments on pereopod 7. Both species occur under wrack and other debris along the banks of estuaries and sheltered lagoons. Orchestia gammarellus is found only along the south-west coast, in the Berg River Estuary, Langebaan Lagoon and the Diep River Estuary. Previous records from Knysna were misidentifications of P. platensis. Platorchestia platensis is found at one west coast site, Langebaan Lagoon, but is widely distributed from Cape Point eastwards to at least Algoa Bay. Both species share the same habitats as several other native beachfleas and competitive interactions between native and introduced species merit investigation.     

Diagnosis of Canine Brucellosis: Comparison between Serological and Microbiological Tests and a PCR Based on Primers to 16S-23S rDNA Interspacer

A pair of primers directed to 16S-23S rDNA interspacer (ITS) was designed directed to Brucella genetic sequences in order to develop a polymerase chain reaction (PCR) putatively capable of amplifying DNA from any Brucella species. Nucleic acid extracts from whole-blood from naive dogs were spiked with decreasing amounts of Brucella canis RM6/66 DNA and the resulting solutions were tested by PCR. In addition, the ability of PCR to amplify Brucella spp. genetic sequences from naturally infected dogs was evaluated using 210 whole-blood samples of dogs from 19 kennels. The whole-blood samples collected were subjected to blood culture and PCR. Serodiagnosis was performed using the rapid slide agglutination test with and without 2-mercaptoethanol. The DNA from whole blood was extracted using proteinase-K, sodium dodecyl sulphate and cetyl trimethyl ammonium bromide followed by phenol–chloroform purification. The PCR was capable of detecting as little as 3.8 fg of Brucella DNA mixed with 450 ng of host DNA. Theoretically, 3.8 fg of Brucella DNA represents the total genomic mass of fewer than two bacterial cells. The PCR diagnostic sensitivity and specificity were 100%. From the results observed in the present study, we conclude that PCR could be used as confirmatory test for diagnosis of B. canis infection.     

Ecology of southern African estuaries: Part XIII. The Palmiet River estuary in the south-western Cape

The Palmiet estuary is only 1,67 km in length, but has a relatively large catchment of 539 km². Rain falls largely during winter when it scours the estuary and widens the mouth, but by late summer the mouth narrows and, in some years, may close. The estuary never closes for more than a few months, and salinities are normal. The entire estuary has clear bottom water and high bottom salinities, but the surface waters are usually fresh and darkly stained with humic acid. This permanent stratification allows many marine species to penetrate the estuary.

Owing to the scouring in winter, the sediments are coarse, >98% consisting of sand and gravel, with a low organic content. In situ primary production is low, contributing only about one fifth of the organic material in the system, the rest being imported from the river and the sea. Species richness is low, only 28 species of invertebrates being recorded, but many of these occur in enormous numbers. Callianassa kraussi plays a crucial role, trapping fine particles around its burrows and locally enhancing the organic content of the sediments. Its faeces are an important vehicle for the concentration and turnover of organic matter.

Four of the 19 species of fish recorded breed in the estuary, the rest being represented largely by juveniles or small adults. Only three species occurred in the estuary year-round, the other species being absent during the period that the estuary is in flood.

Floods and a relatively high flow of river water are critical to keeping the estuary open. If, as proposed, a dam is built on the river, steps will have to be taken to ensure that the mouth is kept open. A related problem will be the reduction of organic material entering the estuary from the river and the sea.     

The life-cycle and seasonal abundance of Echinoparyphium montgomeriana n. sp. (Trematoda: Echinostomatidae) in Natal,South Africa

A new species of Echinoparyphium, E. montgomeriana, is described from Durban, South Africa. Certain unusual aspects of its morphology are discussed. The adult fluke is a parasite of the alimentary tract of birds. Its larval stages are common parasites of freshwater snails belonging to the family Planor-bidae including Buiinus africanus,the intermediate host ofSchistosoma haematobium and S. mattheei in the area. These intra-molluscan stages are shown to have a seasonal transmission cycle.     

Inshore small-mesh trawling survey of the Cape south coast. Part 2. Occurrence of estuarine-associated fishes

Marine inshore trawl catches of 12 species of estuarineassociated fishes are reported together with their depth and length distributions. Underwater observations of four additional species, made during 122 SCUBA dives over rocky substrates, are also recorded. Species include Rhabdosargus holubi, R. globiceps, Pomadasys commersonii, Argyrosomus hololepidotus, Pomatomus saltatrix, Galeichthys sp. and Sarpa salpa. The data are related to published accounts of the occurrence of juveniles of estuarine-associated and inshore species in the Cape south coast region. This revealed three ecologically distinct groups of inshore fishes: typically marine species that only use marine inshore nursery grounds; an intermediate group that uses mainly marine inshore nurseries plus estuarine nurseries to a limited extent; and a specialized group that is dependent on estuarine nurseries. Possible reasons for this dependence on estuaries are suggested.