Experiences with the silicone T‐tube in man and dog

Summary

Silicone T‐tubes are particularly useful for long‐term stenting of the laryngotracheal airway. Silicone is useful when non‐autogenous non‐reactive soft material is wanted for use in the human and animal body. Two case reports illustrate the use of the silicone T‐tube.     

Evaluation of newly developed ‘six recombinant secretary proteins based ‘cocktail ELISA’ and ‘whole cell lysate’ based ‘indigenous ELISA’ and tissue microscopy’ with ‘Gold standard’ histo-pathology for the diagnosis of Johne’s disease in slaughtered goats and buffaloes

Three screening tests {(newly developed, six recombinant secretory proteins based 'cocktail ELISA', in-house robust 'indigenous ELISA' based on semi-purified protoplasmic antigens and tissue microscopy were evaluated with 'Gold standard', histo-pathology for the diagnosis of Johne's disease in goats and buffaloes. Serum and tissues {mesenteric lymph nodes and intestines) were driven from farmer's goats (n = 77) and buffaloes (n = 40) slaughtered for harvesting meat and farm goats (n = 77), died and necropsied. Twenty seven (35%) goats and 23 (57.5%) buffaloes were positive in all the four tests. Of 134 tissues screened by histo-pathology, 79.8% MLN and 76.8%, intestines, were positive for MAP infection. In tissue microscopy, 55.2 and 52.3%, goats and buffaloes were positive, respectively. Of 117 sera screened by i_ELISA, 58.4 and 70.0%, goats and buffaloes were positive, respectively. Whereas, c_ELISA detected 55.8 and 62.5%, goats and buffaloes, positives, respectively. Twelve tissues (70.5%) of goats necropsied were positive, both in tissue microscopy and histo-pathology. Most significant gross findings were serous atrophy of the fat and mild to moderate, diffuse thickening of terminal ileum, especially at ileo-caecal junction with or without transverse / longitudinal corrugations. In histo-pathology grade III and IV lesions were significantly low as compared to grade I and II. Of the four tests used for screening 268 samples, histo-pathology was most sensitive (78.3%), followed by i_ELISA (62.3%), c_ELISA (58.9%) and tissue microscopy (58.9%). Between two ELISA tests, c_ELISA using six recombinants secretory proteins, had higher specificity as compared to i_ELISA.     

Dietary Supplements of Vitamins E and C and β-Carotene Reduce Oxidative Stress in Cats with Renal Insufficiency

Oxidative stress may contribute to the progression of chronic renal failure. In this study, cats with spontaneous renal insufficiency were fed a dry cat food supplemented with the antioxidants vitamins E and C, and β-carotene for 4 weeks. When compared with healthy cats, cats with renal insufficiency had a tendency to oxidative stress. The antioxidant supplements significantly reduced DNA damage in cats with renal insufficiency as evidenced by reduced serum 8-OHdG and comet assay parameters. Therefore, supplements of vitamins E and C and β-carotene as antioxidants may be beneficial to cats with renal disease. Partial data of this study were presented at the Experimental Biology meeting on 17–21 April, 2004. Washington DC, USA     

Transcatheter closure of a small atrial septal defect with an Amplatzer™ patent foramen ovale occluder in a working dog with cyanosis and exercise intolerance at high altitude

A 6.5-year-old male Border Collie presented for transcatheter closure of an atrial septal defect due to exercise intolerance and cyanosis while working and training at altitude. A small, left-to-right shunting secundum atrial septal defect was confirmed with no evidence of significant right-sided volume overload. Pulmonary hypertension with subsequent right-to-left interatrial shunting occurring during exercise at high altitude was suspected and prompted the closure of the defect due to the dog's continued athletic requirements. The anatomy of the defect prompted use of a patent foramen ovale occluder rather than an atrial septal defect occluder, which was deployed using a combination of fluoroscopic and transesophageal echocardiographic guidance. The owner did not report continued exercise intolerance or cyanosis and the dog's lifestyle and residence at altitude was unchanged.     

In vitro and in vivo gene therapy with CMV vector-mediated presumed dog ��-nerve growth factor in pyridoxine-induced neuropathy dogs

Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog β-nerve growth factor (pdβ-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdβ-NGF protein reacted with a human β-NGF antibody and showed bioactivity in PC12 cells. The pdβ-NGF was shown to have similar bioactivity to the dog β-NGF. The recombinant pdβ-NGF plasmid was administrated into the intrathecal space in the gene therapy group. Twenty-four hours after the vector inoculation, the gene therapy group and the positive control group were intoxicated with excess pyridoxine for seven days. Each morning throughout the test period, the dogs'' body weight was taken and postural reaction assessments were made. Electrophysiological recordings were performed twice, once before the experiment and once after the test period. After the experimental period, histological analysis was performed. Dogs in the gene therapy group had no weight change and were normal in postural reaction assessments. Electrophysiological recordings were also normal for the gene therapy group. Histological analysis showed that neither the axons nor the myelin of the dorsal funiculus of L₄ were severely damaged in the gene therapy group. In addition, the dorsal root ganglia of L₄ and the peripheral nerves (sciatic nerve) did not experience severe degenerative changes in the gene therapy group. This study is the first to show the protective effect of NGF gene therapy in a dog model.     

Studies on ‘zinc deficiency syndrome’ in Black Bengal goats (Capra hircus) fed with fodder (Andropogon gayanus) grown on soil treated with an excess of calcium and phosphorus fertilizer

Overliming and excessive application of superphosphate caused a zinc deficiency in the soil and so reduced the uptake of zinc by fodder plants. Bucks reared on such fodder had significantly (p<0.01) less zinc in their hair compared with controls and suffered from conditioned zinc deficiency syndrome with a significant (p<0.01) loss of body weight, stunted growth, alopecia, lethargy, abnormal (kyphotic) gait, anorexia, digestive and respiratory problems. Oral supplementation with zinc sulphate very rapidly improved these conditions to near normality. Histological examination of samples of skin and testis from the zinc-deficient bucks revealed formation of excessive keratin, retention of nuclei in the stratum corneum and reduction in the width of the stratum granulosum in the skin, while samples of testis indicated degenerative changes, including atrophy of the seminiferous tubules, hyperplasia of the germinal epithelium and thickening of the walls of blood ve ssels.     

Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and γδ T cells in resistant Canaria Hair Breed sheep

Canaria Hair Breed (CHB) sheep are more resistant than Canaria sheep (CS) to experimental Haemonchus contortus infection. Protective responses appear effective against the adult stage of the parasite, not as commonly reported in other breeds against the larval stages. In this study we have quantified several abomasal immune cells and correlated these with parasitological variables for each breed. A significant negative correlation between CD4+ T cell numbers and worm burden or length at 28 dpi was seen only in CS sheep. Significant negative correlations for both abomasal eosinophils and γδ/WC1+ T cells, and fecundity of the adult worms were observed only in the resistant CHB sheep breed. Tissue eosinophils and γδ/WC1+ T cells were positively correlated in CHB sheep. We suggest that the two sheep breeds have disparate immune responses following infection with the parasite and that γδ+ T cells in association with eosinophils may play a hitherto unrecognised role in modulating fecundity in H. contortus adult female parasites.     

Molecular detection and phylogenetic analysis of ‘Candidatus Bartonella merieuxii’ in dogs and its effect on hematologic parameters

Emerging Bartonella spp. infection can result in clinical symptoms such as endocarditis in humans and animals. This study analyzed the genetic phylogeny of the Bartonella spp. circulating in Iranian dogs. Also, this is first study on the relationship of Bartonella spp. and haematological factors from dogs in Fars. Ninety-eight blood samples were collected from the dogs of Fars province, Iran. Two different PCRs targeting rpoB gene and ITS sequence of Bartonella spp., followed by sequencing were performed. In addition, CBC and the differential count of WBC were determined. The “prevalence” of Bartonella spp. was 12.2 % (95 % CI: 5.72–18.68 %) in this population and the sequences matched with a newly proposed species; ‘Candidatus Bartonella merieuxii’. A significant increase in WBC due to neutrophilia and decreased RBC, Hct, and Hb concentrations were detected in Bartonella spp. infected dogs. The close contact between humans and dogs, and the zoonosis potential of Candidatus Bartonella merieuxii, emphasize on the need for more studies on ‘Candidatus Bartonella merieuxii’.     

Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in broilers and workers at ‘pluck shops’ in Trinidad

Tropical Animal Health and Production - Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a cause of zoonotic infections in many countries. People with occupational...     

Appraisal of the ‘penumbra effect’ using lingual pulse oximetry in anaesthetized dogs and cats

ObjectiveFactors described as contributors to the ‘penumbra effect’ in relation to pulse oximetry include optical shunting, circulatory anastomoses and probe parallelity. This study aimed to clarify the main underlying mechanism involved.Study designProspective clinical trial.AnimalsA total of 30 dogs and 15 cats (client-owned).MethodsIn anaesthetized dogs and cats, a pulse oximeter probe was placed on the tongue to measure haemoglobin oxygen saturation (SpO₂) and perfusion index. In 15 dogs, the probe was positioned at the root (baseline) of the tongue, then at 0.5 and 1 cm rostral to it, to investigate the effect of circulatory anastomoses on SpO₂ values. In cats (which do not have lingual arteriovenous anastomoses), the probe was positioned at the root and apex of the tongue. To assess the effect of probe parallelity on SpO₂ values in dogs, two lines were drawn parallel to the planes of the light-emitting diode and the detector surfaces and the intersection angle calculated using ImageMeter Pro, Google Play. In a further 15 dogs, the probe was placed at the tongue edge (0% optical shunt), with 50% optical shunt, then with the 50% optical shunt shielded. Data were analysed using Friedman’s test, Student t test and Pearson’s correlation coefficient (p < 0.05).ResultsIn dogs, SpO₂ values were significantly higher at 1.0 cm than at baseline (p < 0.0001). In cats, there were no significant differences in SpO₂ values at each location. There was no significant difference in SpO₂ between 0% and 50% optical shunt in dogs. SpO₂ had a moderate negative correlation with tongue thickness and negligible correlation with intersection angle.Conclusions and Clinical relevanceCirculatory anastomoses are probably responsible for observed changes in SpO₂ as the probe is placed towards an extremity, rather than optical shunting or probe parallelity.     

Efficacy of ‘indigenous vaccine’ using native ‘Indian bison type’ genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne’s disease in an organized goat herd

Therapeutic efficacy of a new ‘Indigenous vaccine’ prepared from native highly pathogenic ‘Indian Bison Type’ genotype of Mycobacterium avium subspecies paratuberculosis (MAP) of goat origin has been evaluated with respect to control of clinical Johne’s disease in naturally infected Mehsana breed of goat in North Gujarat. Fifty goats from Sheep and Goats Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, were randomly divided into 2 groups viz.,‘Vaccinated’(n?=?35) and ‘Control’(n?=?15). After vaccination, goats were monitored for physical condition, morbidity, mortality, body weights, shedding of MAP in feces, internal condition, gross lesions and humoral immune responses up to 120 days (at each interval of 30 days). At the end of 120 days trial, there was marked overall improvement in physical condition and body weights of vaccinated goats as compared to ‘Control’ goats. Vaccinated goats gained significantly (P?<?0.05) higher body weights, hardly exhibited any lesions characteristic of JD, had significantly higher (P?<?0.01) antibody titers and shedding of MAP was significantly (P?<?0.01) reduced. Few of the vaccinated goats were positive for MAP DNA in faecal PCR and blood PCR before vaccination. However, all were found as negative at 120 days post vaccination (DPV). Overall vaccine exhibited effective in restriction of MAP infection and significant improvement in production parameters and reduction in mortality and morbidity due to JD. The trial in the herd will be continued.     

Spread of an enteric ‘marker’ organism during evisceration of New York dressed poultry in a simulated kitchen environment

1. Five volunteers, with experience of eviscerating poultry or game birds in the home, each eviscerated three New York dressed chicken carcases that had been artificially inoculated in the colon with a readily identifiable ‘marker’ strain of Escherichia coli.

2. In all cases, evisceration resulted in breakage of the intestines at one or more sites, often with leakage of gut contents, or extrusion of faeces from the cloaca because of pressure on the colon during the manipulations involved.

3. The marker organism was detected in the vent region and abdominal cavity of each carcase and sometimes on the breast and back, which appeared to reflect the degree of handling during evisceration, because the hands of each individual became heavily contaminated.

With some individuals, the marker also spread beyond the immediate preparation area and was detected on exposure plates.

5. Results support the view that evisceration of poultry in a domestic environment could lead to cross‐contamination of other foods with any food‐borne human pathogens present.