The influence of lime and nitrogen fertilisers on spore counts of Pithomyces chartarum in pasture

AIMS: To determine whether the application of lime or nitrogen to pasture affected the spore counts of Pithomyces chartarum.

MATERIALS AND METHODS: The lime application studies were undertaken on a spring-calving, pasture-based, commercial dairy farm near Te Awamutu, New Zealand. On 6 November 2012, five randomly selected paddocks were split into three equal sections. In two of the sections, lime was applied at either 1.5 or 2.5?t/ha, and the central section was left as an untreated control. Each section was sampled for spore counting weekly from 16 January to 15 May 2013.

Starting in January 2013, five other randomly selected paddocks were monitored for spore counts. On 20 March 2013 the average spore counts in three paddocks were >100,000 spores/g of pasture. These paddocks were then divided into three equal sections and lime was applied as described above. Spore counting in each section continued weekly until 15 May 2013.

The nitrogen application study was carried out on three commercial dairy farms near Te Awamutu, New Zealand. Two randomly selected paddocks on each farm were divided into three equal sections and, on 20 December 2012, nitrogen in the form of urea was applied at either 50 or 80?kg urea/ha to two of the sections; the central section remained as an untreated control. Each section was sampled for spore counting weekly from 16 January to 15 May 2013.

RESULTS: Following pre-summer lime application, treatment at 1.5 or 2.5?t/ha did not affect spore counts over time compared with the control section (p>0.26). Similarly following autumn lime application, treatment at 1.5 or 2.5?t/ha did not affect spore counts over time compared with the control section (p>0.11). Following nitrogen application median spore counts remained <20,000 spores/g pasture throughout the trial period and there was no effect of treatment on spore counts over time (p>0.49).

CONCLUSION: This study found that application of lime before the risk period for facial eczema, in November, application of lime after a spore count rise, in March, or urea application in December did not affect changes in number of spores produced by P. chartarum.

CLINICAL RELEVANCE: This study does not support previous suggestions that fertilising pasture with lime or urea could alter the spore counts of P. chartarum. Fertiliser use does not provide an alternative to, or support, conventional methods of facial eczema control such as zinc prophylaxis or treatment of pasture with fungicides.     

Remembrances of Jacques Jenny, DrMedVet 1917–1971

Editor's note : The holding of the 19th Annual Meeting of the American College of Veterinary Surgeons in Philadelphia in conjunction with the Centennial Celebration of the School of Veterinary Medicine of the University of Pennsylvania provides an opportunity for reflection. Dr. Jacques Jenny, Professor of Orthopedic Surgery at the School, was Chairman of the Organizing Committee that gave rise to the American College of Veterinary Surgeons on December 16,1965, and was elected as first President of the College. The solid foundation and high ideals that the Organizing Committee established are in large part responsible for the success of the College. Dr. Jenny died on November 20, 1971. Those of us fortunate to have known him personally realize the immense gap that his premature death left. For those that did not know him, I hope that this article permits some appreciation of the contributions of Dr. Jenny the comparative orthopedic surgeon, as well as of the very special human qualities of Jacques Jenny the man. The first author, Dr. Mark Allam, was Professor of Surgery and Dean of the School of Veterinary Medicine during most of Dr. Jenny's career at the University of Pennsylvania, and was the first Chairman of the Board of Regents of the American College of Veterinary Surgeons. The second author, Dr. David Nunamaker, is the first holder of the Jacques Jenny Professorship in Veterinary Orthopedics at the University of Pennsylvania.     

Obituary

Extract

It is with deep regret that we learn of the death at Palmerston North, in his eighty-first year, of William Maurice Webster. ‘Wally’ as he was affectionately known, really had no desire to enter the veterinary profession, but his father (himself a veterinarian and farmer from Wiltshire County in England) was a man of firm views concerning the career his son should follow. It was thus that in 1922, after 4 years study at the Royal Dick Veterinary College and Edinburgh University, the young Webster completed concurrently the necessary qualifications for both Membership of the Royal College of Veterinary Surgeons and the Bachelor of Science degree, the latter with a major in medical physiology.     

Bovine tuberculosis and milk production in infected dairy herds in Ireland

This study describes the relationship between bovine tuberculosis (TB) and milk yield in TB-infected dairy herds in Ireland. The study had two objectives: to determine whether cows detected as TB reactors (and thus subject to immediate slaughter) were likely to be the higher milk-producing cows, and to determine whether subclinical TB infection was associated with reduced milk production at or around the time of disclosure (detection). All Irish dairy herds restricted from trading between the 1^st June 2004 and the 31^st May 2005 as a result of two or more TB reactors by the Single Intradermal Comparative Tuberculin Test (SICTT) were considered for study. The data consisted of 419 herds. Data were collected on all TB reactors and a random sample of 5 non-reactor cows in these herds: a data set of 4340 cows (2342 TB reactors and 1998 non-reactors). Previous milk data for the cows were taken into consideration and thus all lactations on a cow were analysed together with the years of lactations. There was an inherent hierarchical structure in the data, with lactations nested within cows and cows within herds and thus a linear mixed model with two random effects was used to describe the data. The results of this study showed that for all lactations and years under investigation, milk yield was significantly lower for TB reactor cows, with differences ranging from 120 kg (2003, lactation 3) to 573 kg (2001, lactation 1), when compared to the non-reactor cows.     

From Osler to Olafson. The evolution of veterinary pathology in North America

Most branches of biological science in North America developed first in the United States, and later were taught and practiced in Canada. An exception was veterinary pathology, which as a discipline taught in veterinary colleges and as a field of research, developed first in Canada, and from there crossed the border to the United States. Pathology was first taught at the Montreal Veterinary College, founded in 1866 by Duncan McEachran, a graduate of the Edinburgh Veterinary College. From the outset, he formed a close association with the medical faculty of McGill University, permitting his students to attend the same classes in the basic subjects with the medical students. Eventually, the Montreal Veterinary College became formally affiliated with McGill University, as the Faculty of Comparative Medicine and Veterinary Science. The McGill veterinary faculty was forced to close for economic reasons in 1903, but it left an enduring legacy, particularly in the field of veterinary pathology. The legacy, a novel concept in the 1870's, was that pathology was the cornerstone of a veterinary education; the place where anatomy, physiology, chemistry and botany met with the clinical subjects, and gave the latter meaning. This tradition was formed at the Montreal Veterinary College by the world renowned physician William Osler, North America's leading medical teacher, whom McEachran had invited to teach at the College in 1876 in addition to his duties in the faculty of medicine. Osler had studied with Virchow in Berlin and applied his methods of autopsy technique and of scientific inquiry to his teaching of both human and veterinary pathology at McGill. Osler also undertook investigations into various diseases of domestic animals, at the request of McEachran, who doubled as Chief Veterinary Inspector for the Dominion Department of Agriculture. Osler left McGill University in 1884. Only after that year did other North American veterinary schools adopt pathology as a discipline of instruction. However, by 1884, Osler had already left his indelible imprint on the students (both medical and veterinary) he had taught in Montreal, one of whom took over the teaching of pathology in the veterinary college. Another, who followed Osler's example and also studied in Berlin with Virchow, wrote the first book in the English language on veterinary post mortem technique in 1889.(ABSTRACT TRUNCATED AT 400 WORDS)     

Effects of 12 and 17 cmH2O positive end-expiratory pressure applied after alveolar recruitment maneuver on pulmonary gas exchange and compliance in isoflurane-anesthetized horses

Objective

To compare static compliance (C_st) and alveolar–arterial oxygen tension difference [P(a–a)O₂] between positive end-expiratory pressures (PEEP) of 7, 12 and 17 cmH₂O applied after an alveolar recruitment maneuver (RM) in isoflurane-anesthetized horses.

Systemic Concentrations of Endogenous and Exogenous FSH in Anoestrous Ewes Superstimulated with Folltropin®-V

The synchronization of follicular waves with medroxyprogesterone acetate (MAP) and oestradiol‐17β (E₂‐17β) prior to ovarian superstimilation in anoestrous ewes reduces the variability in superovulatory responses by an unknown mechanism. Follicle stimulating hormone (FSH) is a primary promoter of antral follicular development, but the relevance of circulating FSH concentrations to the superovulation performance in ewes has not been examined. Eighteen anoestrous Rideau Arcott ewes (May–June) were superovulated with Folltropin^®‐V (porcine FSH), with (n = 8; treated ewes) or without (n = 10; control ewes) a single i.m. dose of 350 μg of E₂‐17β, given on the sixth day of a 14‐day treatment with MAP‐releasing intravaginal sponges (60 mg). The superovulatory treatment, begun 6 days after E₂‐17β injection, consisted of six i.m. applications of Folltropin^®‐V given twice daily (at 08:00 and 16:00 h), followed by an i.m. injection of GnRH (50 μg). Blood samples collected every 8 h throughout the 3‐day treatment, were analysed by radioimmunoassays for concentrations of ovine and porcine FSH, using species‐specific standards and primary antibodies. Serum concentrations of oFSH were greater (p < 0.05) in the controls compared to treated ewes at 40, 64 and 72 h and the variability in mean oFSH concentrations was greater (p < 0.05) in control ewes at 40, 48, 64 and 72 h after the 1^st Folltropin^®‐V injection. There were no differences (p > 0.05) between the two groups in serum concentrations of pFSH. Significant correlations were recorded between the number of corpora lutea (CL) and oFSH concentrations at 8 h (r = 0.72, p < 0.05), 16 h (r=0.63, p < 0.05) and 64 h (r = 0.84, p < 0.01) after the 1^st Folltropin^®‐V injection. The total number of recovered embryos was positively correlated to oFSH concentrations at 56 h (r = 0.69, p < 0.05). We concluded that changes in endogenous FSH concentrations during ovarian superstimulation with pFSH might contribute to the variability in superovulatory responses in ewes.     

Effect of milk feed source,frequency of feeding and age at turnout on calf performance,live-weight at mating and 1st lactation milk production

Female calves (n = 108) were assigned to 6 cold milk feeding treatments in two experiments for a 70-day period. Live-weight (LW) was measured weekly, with an additional LW taken at day 410 and post-calving for animals in experiment 1. In Experiment 1, the effect of feeding frequency and age of turnout to pasture on calf performance and 1^st lactation milk yields were evaluated. The whole milk (WM) feeding treatments applied were (i) once daily feeding (OD), (ii) twice daily feeding (TD), (iii) OD feeding, outdoors at 38 days (ODO). In Experiment 2, the effects of feeding milk replacer (MR) as opposed to WM and age of turnout to pasture on calf performance were evaluated. The treatments applied were (i) OD feeding with WM (OD), (ii) OD feeding with milk replacer (MR) (ODMR), (iii) OD feeding with MR, outdoors at 38 days (ODMRO). Experiment 1: There were no differences (P > 0.05) in LW or average daily gain between TD and OD calves at day 80 or 410. ODO calves had lower LW at day 80 as compared to OD or TD (P < 0.001). Calf LW at day 80 was 86, 89 and 85 kg and at day 410 was 304, 309 and 316 kg for OD, TD and ODO, respectively. Milk feeding frequency or time of calf turnout had no effect on LW post calving, milk composition or 1st lactation milk yields. Experiment 2: Total LW at day 80 was higher (P < 0.05) for ODMR compared to OD or ODMRO calves. Calf LW was 87, 95, and 88 kg for OD, ODMR and ODMRO, respectively. However, LW at day 410 did not differ between treatments.This study showed that while some differences were observed in calf LW at day 80, these differences had no effect on LW at day 410 or 1^st lactation milk yield. It can be concluded that calves can be successfully reared when fed OD with WM or MR, indoors and when turned out to pasture at 38 days of age.     

Obituary - Dudley Arthur Gill

Extract

D. A. Gill died in Sydney on May 1, 1973, at the age of 72. He received his training at the Royal Veterinary College, London, where he qualified in 1922. He then did a Diploma of Veterinary State Medicine at Edinburgh. He joined the New Zealand Department of Agriculture as a Veterinary Officer and from 1928 he was second in charge at the Wallaceville Veterinary Laboratory. While he was at Wallaceville he did some first-class research on the problems of listeriosis and enterotoxaemia. The house that he built at Wallaceville he later sold to his friend Dr I. J. Cunningham.     

Comparative immune response and pathogenicity of the H9N2 avian influenza virus after administration of Immulant®, based on Echinacea and Nigella sativa,in stressed chickens

Avian influenza vaccines are commonly used in the poultry industry, and some medicinal plants can increase the efficacy of such vaccines. The objective of this study was to evaluate the effect of Immulant^® (IMU) (a commercial product based on Echinacea and Nigella sativa) on stress induced by dexamethasone (DEX) in chickens vaccinated (VAC) against the H9N2 avian influenza virus (AIV-H9N2). Seven experimental groups were included: the negative control, VAC, DEX, VAC + DEX, VAC + DEX + IMU, VAC + IMU and IMU groups. The vaccinated chickens (at 10 days of age) were injected daily with DEX for three days pre-vaccination and for three days pre-challenge and orally administered 1% IMU for 6 weeks post-vaccination (PV). The chickens were then challenged intranasally with AIV-H9N2 at 28 days PV. Serum, blood, tracheal and cloacal swabs and tissue samples were collected in the 1^st and 4^th weeks PV and at different time points post-challenge. The results showed significant changes (P ≤ 0.05) in oxidative stress and antioxidant biomarkers (malondialdehyde, nitric oxide and reduced glutathione), haematological and immunological parameters, final live weights, relative organ weights and histopathological lesions between the VAC+DEX group and the VAC group. Moreover, IMU significantly increased protection rates post-challenge, HI antibody titers and heterophil phagocytic activity and decreased DEX-induced stress and virus shedding titers. In conclusion, oral administration of 1% IMU for six weeks can enhance the immune response after AI-H9N2 vaccination and reduce the pathogenicity of infection in stressed chickens.     

All-purpose veterinary education: a personal perspective

The Recognition Lecture is an annual honor awarded by the Association of American Veterinary Medical Colleges (AAVMC) to an individual whose leadership and vision have made significant contributions to academic veterinary medicine and the veterinary profession. In 2011, this prestigious honor was awarded to Dr. Peter Eyre, Dean Emeritus of the Virginia-Maryland Regional College of Veterinary Medicine (VMRCVM). Dr. Eyre is a fierce advocate for veterinary medical education, with a clear vision of its value in ensuring that veterinarians are well positioned to meet societal needs. Dr. Eyre possesses an international perspective regarding the challenges and problems facing veterinary medical education and has a keen eye for getting to the heart of these challenges. He is known to ask hard questions and propose difficult choices. Dr. Eyre received his undergraduate veterinary degree (BVMS), bachelor of science degree, and PhD from the University of Edinburgh. He was Lecturer in Pharmacology at the Royal (Dick) School of Veterinary Studies for seven years before joining the faculty of the University of Guelph's Ontario Veterinary College, where he was Chair of the Department of Biomedical Sciences and Associate Director of the Canadian Centre for Toxicology. Dr. Eyre was appointed Dean of the VMRCVM in 1985, where he established the Center for Government and Corporate Veterinary Medicine in 1989. After retiring in 2003, he was named Interim Dean of the University of Calgary's new veterinary school. Among his many awards are the Norden Distinguished Teacher Award and the Sigma Psi Excellence in Research Award. In 2008 the American Veterinary Medical Association (AVMA) honored him with the President's Award, and in 2010 the University of Edinburgh awarded him the honorary degree of Doctor of Veterinary Medicine and Surgery. The Peter Eyre Student Leadership Award at the VMRCVM and the Peter Eyre Prize in Pharmacology at the University of Guelph are both named in his honor. He is a past president of the AAVMC, a fellow and former board member of the American Academy of Veterinary Pharmacology and Therapeutics, and a former member of the AVMA Legislative Advisory Committee. In the following article, Dr. Eyre offers his insights on the current debate about the future of veterinary medical education.     

Dietary Mannanoligosaccharide Supplementation Improves Growth Performance,Intestinal Integrity,Serum Immunity,and Antioxidant Capacity of Partridge Shank Chickens

Herein, we assessed the impact of dietary addition of konjac mannanoligosaccharide (MOS) on the growth, intestinal morphology, serum immune status, and oxidative status in Partridge Shank chickens. For the experiment, one-day-old chicks (n=192) were randomized into six replicates (n=8/replicate) and fed four different diets: a basal diet containing 0 (Control group), 0.5, 1, or 1.5 g MOS per kg of diet (g/kg) for 50 d. Relative to the control, the group fed 0.5 g/kg MOS decreased feed consumption from 22^nd to 50^th d and 1^st to 50^th d (P<0.05). By adding MOS, the height of the intestinal villus and the villus height to crypt depth ratio were increased (P<0.05); 1.5 g/kg MOS was the best dosage for these parameters. Jejunal and ileal goblet cell density increased following MOS supplementation at 21 d (P<0.01) and 50 d in the jejunum (P<0.05), respectively. Moreover, adding MOS to the diet increased the contents of IgA and IgM at 21 d (P<0.05) and total antioxidant capacity (P<0.05) at 50 d in the serum but decreased malondialdehyde content (P<0.01) at 21 d in the group fed 0.5 and 1.5 g/kg MOS. The findings suggested that MOS supplementation could affect feed consumption, intestinal health, serous immunity, and antioxidant capacity of Partridge Shank chickens.     

Listeria monocytogenes septicemia in an immunocompromised dog

An 11‐year‐old, male castrated, Boston Terrier was presented to the North Carolina State University College of Veterinary Medicine Small Animal Emergency Service with a 2‐day history of progressive ataxia, left‐sided head tilt, and anorexia. The dog had previously been diagnosed with chronic lymphoid leukemia and suspected immune‐mediated destruction of his bone marrow precursor cells, possibly due to therapy with immunosuppressive dosages of prednisone and azathioprine. During the physical examination, abnormal findings included an increased body temperature and horizontal nystagmus. Diagnostic investigations included a computed tomography (CT) scan, which confirmed bilateral otitis media, and a blood culture, which was positive for Listeria monocytogenes serotype 4b (epidemic clone 1). Upon treatment with ampicillin/sulbactam, enrofloxacin, and minocycline, the dog became normothermic and the neurologic signs improved. L monocytogenes serotype 4b (epidemic clone 1) has been associated with outbreaks of human listeriosis originating from food contamination. Although rare case reports of Listeria spp. infection in dogs exist, an actual infection with the epidemic clone 1 strain has never before been reported in a dog. It should be included in the differential diagnoses in immunocompromised dogs with clinical signs of septicemia.     

The Effects of Verapamil Hydrochloride on Electrocardiographic (ECG) Parameters of Domestic Donkey (Equus asinus)

Verapamil hydrochloride, a calcium channel blocker, has been used in domestic animals. Its effects on electrical activities of the heart of donkeys were studied in this preliminary research work. This experiment was carried out on 10 healthy donkeys ranging in age from 4 to 7 years and mean body weight of 155 ± 18.6 kg. The electrocardiogram (ECG) was recorded on a base apex lead before (for 3 days), during, and after verapamil injection (10, 20, 30, 40, 50, and 60 minutes). Verapamil hydrochloride (0.3 mg/kg body weight) was given via catheterizaion as an intravenous bolus over 2 minutes. Heart rate, rhythm, ECG morphology, amplitude, durations, and intervals were measured and taken into consideration and values were compared. Results showed that heart rate was significantly increased at the time of injection from 55 ± 10.7 before injection to 77.61 ± 12.6 beats per minute after injection (P < .05). Duration of P and T waves; P-R interval, and also P-R segment were increased (P < .05). However, duration of QRS complex, P-P and R-R intervals, and S-T segment were significantly decreased after verapamil injection (P < .05). Sinus arrest (2 cases), bradycardia (1 case), and wandering pacemaker (1 case) were recorded before treatment and verapamil injection did not affect these arrhythmias, except in one case in which sinus arrest disappeared. However, after injection of verapamil, four types of arrhythmias (wandering pacemaker [WPM], sinus tachycardia, 1^st degree AVB, and 2^nd degree AVB) were recognized. These arrhythmias (with the exception of tachycardia) can be related to the increase of vagal tone activity that might be attributed to the negative effect of verapamil on SA and atrioventricular block [AV] nodes.     

江苏某奶牛场荷斯坦牛和娟姗牛泌乳性能的比较分析

[目的]研究旨在比较荷斯坦牛和娟姗牛泌乳性能的差异.[方法]收集了江苏某奶牛场2020年1—12月间共896条DHI记录,用多因素方差法分析不同测定季节、胎次、泌乳阶段和产犊季节荷斯坦牛和娟姗牛泌乳性能的差异.[结果]结果表明,不同测定季节、胎次、泌乳阶段和产犊季节对荷斯坦牛和娟姗牛泌乳性能有极显著影响.不同测定季节、...     

Wm C. Barry,M.R.C.V.S.

Extract

The death occurred in Wellington on August 19, 1971, of William C. Barry, a respected member of the veterinary profession. Mr Barry, who was in his 87th year, was born in County Cork and graduated from the Dublin Veterinary College in 1907 with distinction in a number of subjects. He became a Member of the Royal College of Veterinary Surgeons from that date, his membership thus encompassing a period of 64 years.     

Effect of maternal cottonseed feed on the immune and antioxidant status of Santa Ines lambs

Cottonseed has been used as a nutritional alternative in animal production. However, consequences of this nutrient in the progeny is not well characterized. Thus, this work evaluated the effect on the immune and antioxidant status of the progeny of feeding Santa Ines ewe with or without cottonseed. Twenty-four Santa Ines ewes were distributed in two feeding regimes: cottonseed (CS) concentrate (n = 12) and soybean (SB) concentrate (n = 12). After birth, lambs remained with their mothers and blood samples were collected at 1^st, 3^rd, 7^th, 15^th, 30^th and 60^th day of life of 24 lambs born from mothers fed with (CS, n = 12) or without (SB, n = 12) cottonseed. Serum total protein, albumin, alpha beta globulin, gamma globulin, immunoglobulin G and M, activity of glutathione peroxidase (GPx), catalase (CAT), oxygen radical absorbance capacity (ORAC) and variables related to iron metabolism were affected only by sampling times (P < 0.05). The concentration of serum total protein, alpha beta globulin, gamma globulin and immunoglobulin G and M, GPx activity and ORAC values decreased as lamb age increased. Serum albumin concentration and CAT activity, in turn, increased as lamb age increased. In this work, maternal feeding with cottonseed did not affect the serum protein profile and antioxidant status of progeny during the lactation period, indicating no transfer of gossypol effects by milk secretions. Thus, the alternative in ruminants feeding with cottonseed can be used without maternal-descendant effects to immunity and oxidative stress in lambs.     

Antioxidant defence of colostrum and milk in consecutive lactations in sows

Background

Parturition is supposed to be related to oxidative stress, not only for the mother, but also for the newborn. Moreover, it is not clear whether consecutive pregnancies, parturitions, and lactations are similar to each other in regards to intensity of metabolic processes or differ from each other. The aim of the study was to compare dynamic changes of antioxidative parameters in colostrum and milk of sows taken during 72 h postpartum from animals in consecutive lactations. Activities of glutathione peroxidase (GSH-Px), glutathione transferase (GSH-Tr), and superoxide dismutase (SOD), and amount of vitamin A and C were measured. Healthy pregnant animals were divided into 4 groups according to the assessed lactation: A -1^st lactation (n = 10), B - 2^nd and 3^rd lactation (n = 7), C - 4^th and 5^th lactation (n = 11), D - 6^th - 8^th lactation (n = 8). The colostrum was sampled immediately after parturition and after 6, 12, 18 and 36 h while the milk was assessed at 72 h after parturition. Spectrophotometric methods were used for measurements.

Results

The activity of antioxidative enzymes and the concentration of vitamin A increased with time postpartum. The concentration of vitamin C was the highest between the 18th and 36th h postpartum.

Conclusions

Dynamic changes in the values of antioxidant parameters measured during the study showed that sows milk provides the highest concentration of antioxidants in the 2^nd and 3^rd and 4^th and 5^th lactation giving the best defence against reactive oxygen species to newborns and mammary glands.     

Postoperative analgesic effects of either a constant rate infusion of fentanyl,lidocaine, ketamine,dexmedetomidine, or the combination lidocaine‐ketamine‐dexmedetomidine after ovariohysterectomy in dogs

ObjectiveTo evaluate the postoperative analgesic effects of a constant rate infusion (CRI) of either fentanyl (FENT), lidocaine (LIDO), ketamine (KET), dexmedetomidine (DEX), or the combination lidocaine-ketamine-dexmedetomidine (LKD) in dogs.Study designRandomized, prospective, blinded, clinical study.AnimalsFifty-four dogs.MethodsAnesthesia was induced with propofol and maintained with isoflurane. Treatments were intravenous (IV) administration of a bolus at start of anesthesia, followed by an IV CRI until the end of anesthesia, then a CRI at a decreased dose for a further 4 hours: CONTROL/BUT (butorphanol 0.4 mg kg⁻¹, infusion rate of saline 0.9% 2 mLkg⁻¹ hour⁻¹); FENT (5 μg kg⁻¹, 10 μg kg⁻¹hour⁻¹, then 2.5 μg kg⁻¹ hour⁻¹); KET (1 mgkg⁻¹, 40 μg kg⁻¹ minute⁻¹, then 10 μg kg⁻¹minute⁻¹); LIDO (2 mg kg⁻¹, 100 μg kg⁻¹ minute⁻¹, then 25 μg kg⁻¹ minute⁻¹); DEX (1 μgkg⁻¹, 3 μg kg⁻¹ hour⁻¹, then 1 μg kg⁻¹ hour⁻¹); or a combination of LKD at the aforementioned doses. Postoperative analgesia was evaluated using the Glasgow composite pain scale, University of Melbourne pain scale, and numerical rating scale. Rescue analgesia was morphine and carprofen. Data were analyzed using Friedman or Kruskal–Wallis test with appropriate post-hoc testing (p < 0.05).ResultsAnimals requiring rescue analgesia included CONTROL/BUT (n = 8), KET (n = 3), DEX (n = 2), and LIDO (n = 2); significantly higher in CONTROL/BUT than other groups. No dogs in LKD and FENT groups received rescue analgesia. CONTROL/BUT pain scores were significantly higher at 1 hour than FENT, DEX and LKD, but not than KET or LIDO. Fentanyl and LKD sedation scores were higher than CONTROL/BUT at 1 hour.Conclusions and clinical relevanceLKD and FENT resulted in adequate postoperative analgesia. LIDO, CONTROL/BUT, KET and DEX may not be effective for treatment of postoperative pain in dogs undergoing ovariohysterectomy.     

Pain-related aggression in dogs: 12 clinical cases

The aim of this retrospective study was to describe the main features of pain-related aggression in dogs. Twelve dogs presented for aggressive problems at the Veterinary Hospital of the Autonomous University of Barcelona, Spain, were included, and a questionnaire was used to gather information on the context of the aggression, body posture during the attack, impulsiveness, and aggressive behavior before the onset of the pain-eliciting condition. The most common cause of pain was hip dysplasia (66.7%), but no relationship was found between the cause of pain and the characteristics of the aggressive behavior. Dogs were classified as having been aggressive before or after the onset of painful condition. Dogs that had not been aggressive before the onset of the pain-eliciting condition were more impulsive (df = 1, χ² = 5.3, P = 0.0209), showed aggression as a result of manipulation context more frequently (df = 1, χ² = 6, P = 0.0143), and adopted a defensive body posture more frequently (df = 1, χ² = 3.733, P = 0.0533) than dogs that had been aggressive before the onset of pain. These results suggest that previous expression of aggressive behavior has a major effect on the pattern of pain-related aggression in dogs.