Oestrogenic mycotoxin zearalenone and the pasture fungus Fusarium culorum — a reply

Madam:—I refer to R. T. Gallagher's short communication on the “Oestrogenic Mycotoxin Zearalenone and the pasture fungus Fusarium culmorum” (N.Z. vet. J. 33: 37–8).     

MAF takes over TB scheme — A reply

Madam:— I refer to the letter by Joe Tyndel “MAF takes over Tb scheme” (N.Z. vet. J. 34: 138). While not disagreeing with the sentiment of Dr Tyndel's letter which is a welcome first public correspondence about MAF moves towards commercialisation, I do wish to correct a major inaccuracy contained in the letter.     

Urticaria in the horse after anaesthesia

Extract

Sir:- I read with interest the case report, “An unusual reaction in a horse during anesthesia” by I.L. Anderson, N.Z. vet. J. 31:85 (1983), describing the development of generalized urticaria in a horse after induction of anesthesia with guaiphenesin and thiopentone.     

Canine parvovirus

Extract

Sir:- The paper Canine parvovirus in New Zealand: epidemiological features and diagnostic methods by G.W. Horner [N.Z. vet. J. 31 (1983): 164-4] suggests “it is probable that many veterinary clinics were heavily contaminated with CPV” quoting an Australian reference in support of his contention.     

Editorial Note

In the article “Experimental infection of the possum (Trichosurus vulpecula) with Leptospira interrogans serovar balcanica. I. A comparison of laboratory techniques for the detection of leptospiraemia and leptospiruria” by S. C. Hathaway (N.Z.vet.J.29: 121-5) Tables II and III did not relate to the text. All reprints of this article will contain the correct Tables.     

Myxomatosis – A reply

Madam:— In the September issue (N.Z. vet. J. (1985) 33:154), D. and A.K. Dodd, and Messrs Jackson, Mulvaney, Bishop and Jopp write in support of the introduction of myxomatosis into New Zealand, seeing it “as the only means of protecting the fragile environment of the South Island High Country from complete destruction by the rabbit.”     

Unusual condition in sheep pelts

Extract

On February 26, 1956, the Supervising Meat Inspector at Wellington Abattoir telephoned the Chief Diagnostic Officer at Wallaceville to say that there had been a number of lambs reaching the abattoir during the week affected by a “burning” of the skin.     

EFFECTS OF A PREVENTIVE AND SUPPRESSIVE CONTROL SCHEME ON THE DEVELOPMENT OF THIABENDAZOLE-RESISTANCE IN OSTERTAGIA SPP

SUMMARY Three years after the start of an experiment to assess the merits of thiabendazole (TBZ) treatment of trichostrongylid parasites in weaner sheep, field isolates of Ostertagia spp and Trichostrongylus spp were made from weaner sheep treated under one of three treatment schemes. Treatment frequencies were “nil”, “planned” (5 or 6/year) and “regular” (every 3 weeks). In addition an isolate was taken from a group of “tracer” sheep drenched with TBZ every 10 days. Resistance to TBZ was assessed using an in vitro egg hatch assay, pre- and post-treatment faecal egg counts and a controlled anthelmintic efficiency test. Pre- and post-treatment egg counts revealed the presence of TBZ-resistance in field isolates of mixed species. Egg hatch assays indicated a level of resistance for Ostertagia spp which was proportional to the frequency of TBZ treatment. The “planned”, “regular” and “tracer” strains of Ostertagia spp had resistance ratios for eggs of 4, 13 and 15 respectively when compared to the “nil” strain. In the anthelmintic efficiency assay treatment with 44 mg kg-1 and 88 mg kg-1 of TBZ removed 82 and 96% respectively of the total Ostertagia burden (adults and larvae) from the “nil” strain and 30 and 75% respectively from the “planned” strain. The same dose rates against the “regular” and “tracer” strains and additional rates of 132 or 176 mg kg-1 against the “tracer” strain failed to reduce the Ostertagia burden significantly. Intestinal Trichostrongylus spp from all isolates were fully susceptible to TBZ at 44 mg kg-1. Levamisole at 7.0 mg kg-1 was highly effective (99% reduction) against the “tracer” strain of Ostertagia.     

Predictors of Extreme Negative Feelings Toward Coyote in Newfoundland

Human–coyote interactions have occurred since the arrival of the species to the island of Newfoundland in 1985. A mail survey (N = 786) of Newfoundland residents was conducted in 2008. The survey explored negative feelings toward coyotes. A four stage hierarchical multiple regression model examined how the dependent variable, “feelings,” was influenced by four independent blocks of variables: “existence beliefs,” “impact beliefs,” “fear,” and “experience and demographic characteristics.” Together the predictors explained 50% of the variability, with existence beliefs accounting for most of the variation (ΔR² = . 45), followed by impact beliefs (ΔR² = .024) and fear (ΔR² = .018). The experience-demographic block of variables accounted for minimal influence (ΔR² = .003) and was not statistically significant. The remaining variability might be explained by emotions. When exploring human–wildlife interactions it is important to understand the role of affect in the formation of attitudes as feelings influence the tolerance and ultimately the willingness to coexist with wildlife.     

GnRH and prostaglandin‐based synchronization protocols as alternatives to progestogen‐based treatments in sheep

The study investigated, for cycling sheep, synchronizing protocols simultaneously to the standard “P” protocol using progestogens priming with intravaginal devices and gonadotropin. In November 2014, 90 adult Menz ewes were assigned to either the “P” protocol, “PGF” treatment where oestrus and ovulation were synchronized using two injections of prostaglandin 11 days apart or a “GnRH” treatment where the ewes had their oestrus and ovulation synchronized with GnRH (day 0)–prostaglandin (day 6)–GnRH (day 9) sequence. The ewes were naturally mated at the induced oestrus and the following 36 days. Plasma progesterone revealed that 92% of the ewes were ovulating before synchronization and all, except one, ovulated in response to the applied treatments. All “P” ewes exhibited oestrus during the 96‐hr period after the end of the treatments in comparison with only 79.3% and 73.3% for “PGF” and “GnRH” ewes, respectively (p < .05). Onset and duration of oestrus were affected by the hormonal treatment (p < .05); “GnRH” ewes showed oestrus earliest and had the shortest oestrous duration. Lambing rate from mating at the induced oestrus was lower for “P” than for “PGF” ewes (55.6% and 79.3%, respectively; p < .05). The same trait was also lower for “P” than for “PGF” and “GnRH” ewes (70.4%, 89.7% and 86.7%, respectively; p < .05) following the 36‐day mating period. Prostaglandin and GnRH analogue‐based protocols are promising alternatives for both controlled natural mating and fixed insemination of Menz sheep after the rainy season when most animals are spontaneously cycling.     

Cardiac Troponin I in Doberman Pinschers with Cardiomyopathy

Background: Cardiac troponin I (cTnI) is useful for detection of cardiac myocyte damage, but its efficacy in detecting various stages of dilated cardiomyopathy (DCM) in Doberman Pinschers is unclear. Objectives: To evaluate the diagnostic value of cTnI in various stages of DCM in Dobermans. Animals: Six hundred and fifty‐three cTnI measurements of 336 Doberman Pinschers. Methods: Using a longitudinal study design, staging of the disease was based upon 24‐hour‐ambulatory‐ECG (Holter) and echocardiography. A total of 447 cTnI measurements were performed in 264 healthy Dobermans, and 206 cTnI measurements in 75 Dobermans with cardiomyopathy. Eighty‐eight cTnI samples were from dogs with >100 ventricular premature contractions (VPCs)/24 hour, but without echocardiographic changes (“VPC group”). Additional 19 samples originated from dogs with only echocardiographic changes (“ECHO group”), and 56 samples from dogs with both VPCs and echocardiographic changes (“VPC plus ECHO group”). Twenty samples were from dogs with clinical signs (“clinical group”). The group “incipient” included 23 dogs, that were considered to be normal according to Holter and echocardiography at the time of the exam, but that developed DCM within 1.5 years. Results: cTnI values of dogs in all disease groups, including the “incipient” (0.30 ± 0.20) and “VPC group” (0.36 ± 0.34), were significantly (P= .04, P < .001) higher than the control group (0.07 ± 0.16). A cut‐off value of >0.22 ng/mL had a sensitivity of 79.5% and a specificity of 84.4% to detect all forms of cardiomyopathy. Conclusions and Clinical Importance: cTnI measurement is a valuable diagnostic test that can detect cardiomyopathy in dogs that are otherwise clinically normal.     

Differential response of sex‐linked albino (s al ) and silver (S) hens to high and low light intensity

1. Sex‐linked albino (s ^al ) and S (Silver) female chicks were hatched from heterozygous sires from a brown egg‐type line. They were raised in a windowless house and at 17 weeks of age were caged individually in 4 rooms with white fluorescent tubes: two rooms had a “low” light intensity (less than 10 lux) and two had a “high” intensity (average near 400 lux). In total, 157 females completed the experiment.

2. Body weight, sexual maturity, egg weight, the number of abnormal and cracked eggs, efficiency of food utilisation and mortality were not affected either by lighting treatment or by genotype.

3. The genotype X treatment interaction was highly significant for egg number, laying percentage and clutch length to 362 d. The S females were not affected by light intensity. Albino hens of “high” light intensity were superior to the other 3 groups, laying 12 per cent more eggs than non‐albino hens; but their egg production was moderately depressed by “dim” light relative to others.     

Towards an GMO Discipline: Ethical Remarks

The present technological transformation calls for a new kind of vet: he has to accomplish his own institutional tasks bearing in mind the definition of a “good practice” code and also performing educational and informative tasks in zootechny in order to ensure the animal well-being.

Therefore, his contribution can be relevant in developing and interpreting laws related to the protection of animal welfare and further considerations on the ethical and deontological thought, concerning the technological use of animals.

The vet's role is also fundamental in the following areas: further study of bioethical issues related to zootechny and the professional growth of whoever is engaged, in different ways, in productive activities and research on animals.

     

Haemoplasma infection is not a common cause of canine immune-mediated haemolytic anaemia in the UK

Objective : The aim of this study was to investigate whether the two canine haemoplasma species, Mycoplasma haemocanis and “Candidatus Mycoplasma haematoparvum,” are commonly associated with immune-mediated haemolytic anaemia (IMHA) in UK dogs. Methods : Three groups of dogs were recruited to the study: anaemic dogs with primary IMHA (n=37); anaemic dogs not meeting the inclusion criteria for primary IMHA (n=77) and non-anaemic dogs (n=113). DNA was extracted from 100 μl of blood and subjected to real-time quantitative polymerase chain reaction (qPCR) assays for both species of Mycoplasma. Each assay incorporated co-amplification of canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous internal control. Results : Canine GAPDH was successfully amplified by qPCR from all 227 canine blood samples but none contained M. haemocanis or “Candidatus M. haematoparvum” DNA. Clinical Significance : Haemoplasma infection is uncommon in dogs in the UK and no evidence was found that these organisms act as triggers for IMHA.     

A blind test of the serological response of dogs to infection with Taenia ovis

Fifty six dogs of mixed age and sex were acquired from farms in the Otago/Southland region, and maintained at the Hydatid Research Unit, Taieri, where 43 were each fed two Taenia ovis cysts. All were bled fortnightly for six or 12 weeks. Coded sera were sent to Wallaceville Animal Research Centre for testing using ELISA, with antigen from T. ovis scoleces. Dog treatments were identified after all tests were complete. A discriminant level was derived from the mean absorbance value plus three standard deviations of 56 sera taken at time zero and 78 sera from serially bled uninfected dogs. None of these 134 sera registered as a false positive using this discriminant level. The data showed no significant deviation from normality, and the expected frequency of the occurrence of false positives is therefore less than 0.14%. Four weeks after infection 63% of dogs proved to be infected were serologically positive, rising to 78% after 6 weeks. When worms were removed by anthelmintic treatment, ELISA absorbance levels decreased. Four weeks after removal 70% of previously infected dogs remained positive, decreasing to 30% after 6 weeks.

Six weeks after infection the sensitivity of the test was 78%, and the specificity 63%. However, if dogs with positive ELISA absorbance levels, but which did not purge worms, were regarded as having had worms, the respective figures would be 82% and 100%. The latter figures are similar to our previously published laboratory results. The test is of comparable efficiency to arecoline purgation for surveillance, and has the additional advantage of detecting infection in the majority of those dogs that have been infected for three weeks or more but fail to pass worms on purgation, and a substantial proportion of those infected dogs that were treated by their owners prior to presenting them for purgation in order to avoid detection of infection.     

Drug sales and the veterinarian

Extract

Sir,—I was interested to read the letter by R. Jackson, (N.Z. vet. J., 9: 81) and would like to support his views. However, he did not offer a solution to the problem.     

Tail biting in pigs

Extract

Sir:- Referring to a letter (N.Z. vet. J. (1983) 31: 105–6) by C. Larsen on tail biting, I would like to add some theories about the causes of tail biting under the conditions of intensive pig-rearing     

Improved visualization of the lumbar spine nerve roots in dogs using water excitation (ProSet) as opposed to short tau inversion recovery: A retrospective study of two fat suppression MRI sequences

Magnetic resonance imaging fat suppression techniques are commonly used for diagnosis of canine spinal disease, however, studies comparing different techniques are currently lacking. This retrospective, methods comparison study aimed to evaluate water excitation and STIR MRI pulse sequences for visualization of canine lumbar spinal nerve roots. For inclusion, all dogs had to have dorsal planar MRI studies of the lumbar spine using both sequences. Visual grading analysis was used for scoring the following five criteria: degree of fat suppression; nerve root visualization; subjective tissue contrast; presence of noise; and overall better image quality. Scores were independently recorded by three board‐certified veterinary radiologists on two separate occasions, 3‐6 weeks apart. A total of 90 dogs were sampled. A two‐tailed t‐test showed that there were significant differences in all scored parameters (P < 0.00001), with the exception of noise (P = 0.47343), and that the water excitation sequence scored higher in all cases excluding noise. A Gwets AC kappa for intraobserver and interobserver reliability showed “almost perfect” agreement for the nerve roots in both tests (intra: k = 0.88; inter: k = 0.90). Intraobserver agreement was “substantial” for the degree of fat suppression (k = 0.68), subjective tissue contrast (k = 0.75), and overall better image quality (k = 0.76) and it was “fair” for the noise (k = 0.46). Interobserver agreement was “moderate” for the degree of fat suppression (k = 0.53), subjective tissue contrast (k = 0.63), and overall better image quality (k = 0.66) and “slight” for noise (k = 0.25). These findings supported using the water excitation pulse sequence for fat‐suppressed MRI of canine lumbar spinal nerve roots.     

A replyto “logomach”

Madam:— The letter extracted from the Veterinary Record (no reference given) which appears on viii “News and Views” under “Comment” in the October issue of the organ of the NZVA re pleonasm, under the heading “Verbal Tics” indicates that the writer, “Logomach” has become infected. “... the entire body”, “... an almost exact analogy”, “... quite useless” are early signs of his unfortunate condition.     

Response of three class‐IV major histocompatability complex haplotypes to Eimeria acervulina in meat‐type chickens

1. The importance of MHC genes and background genes in controlling disease resistance, including resistance to avian coccidiosis, has not been clarified in meat‐type chickens.

2. The role of class IV MHC genes in resistance to Eimeria acervulina was assessed in F2 progeny of a cross between 2 meat‐type lines, selected divergently for immune response to Escherichia coli.

3. Disease susceptibility was assessed by lesion score, body weight, packed cell volume and carotene absorption.

4. Chickens with the “K” class IV MHC haplotype had lower lesion scores than chickens with “F” and “A” haplotypes.

5. Plasma carotene concentrations were higher in chickens with “K” haplotype and lower in chickens with “F” and “A” haplotypes whereas body weight and packed cell volume were less sensitive measures of Eimeria infection.

6. Eimeria acervulina resistance appears to be associated with MHC class IV genes; information about MHC haplotypes may be useful in selecting for increased resistance of meat‐type chickens to coccidiosis.