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[目的]为研究赤霉素GA4+7对白桦生长的调控机制。[方法]试验利用1个白桦无性系的分株材料,连续2年分别叶面喷施浓度为0.2 g·L~(-1)(T1)、0.4 g·L~(-1)(T2)的GA4+7溶液,对实验苗木的生长情况进行跟踪调查,并对相应顶端生长组织进行转录组测序和差异基因分析。[结果]对2个年份的苗高、地径等性状分析发现,不同处理间苗高与地径的差异均达到显著或极显著水平,GA4+7对高生长有明显的促进作用,2年后的T2白桦苗高较CK提高了19.65%。于6月28日取GA4+7连续处理2年的白桦顶芽开展RNA-seq分析,结果显示:0.4 g·L~(-1)GA4+7(T2)处理与对照(CK)间的差异基因数量最多,即上调表达的基因有181个,下调表达的基因有55个,这些差异基因在叶绿体类囊体膜、红光、远红光及蓝光的细胞响应等方面富集明显。Pathway富集分析显示:氧化磷酸化途径与光合途径中的8条基因呈上调表达,认为施加0.4 g·L~(-1)GA4+7处理后,上述基因的上调表达加快电子传递进程、催化ATP的合成,从而促进白桦光合作用增强,提高苗期生长量。[结论]对白桦外源施加赤霉素GA4+7后,可上调氧化磷酸化途径和光合途径相关基因的表达,进而促进苗木的高和地径生长。研究结果可为白桦生长相关途径基因的克隆提供参考。 相似文献
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为了解外源激素和养分溶液浸种对君迁子(Diospyros lotus)种子发芽的影响,采用L9(34)正交试验设计,开展不同浓度赤霉素(GA3)、吲哚丁酸(IBA)和磷酸二氢钾(KH2PO4)溶液的浸种试验.结果显示,君迁子种子发芽持续38天;各处理的发芽率、发芽势、平均发芽时间和发芽指数均值分别为11.11%~82.78%、5.00%~52.78%、14.70~30.95天和0.22~4.40粒/天,处理间各指标均差异显著或极显著;除T1处理外,其他处理的发芽率均极显著高于CK;处理2~9的各指标均表现较好.GA3和IBA的交互作用是影响各发芽指标的主导因子.极差分析结果显示,GA3、IBA和KH2PO4分别为0.15 g/L、0.45 g/L和0.50%,分别浸种2、2和6 h,可提高君迁子种子的发芽性状.外源激素与养分溶液浸种处理可促进君迁子种子发芽,可应用于生产实践. 相似文献
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油桐花芽分化期内源激素含量的变化 总被引:3,自引:0,他引:3
为给油桐花芽分化及花期调控的研究提供理论依据.在观察油桐花芽形态分化的显微结构的基础上,测定并分析了该时期油桐叶片中内源激素ZR(玉米索核苷)、IAA(生长素)、GA(赤霉素)、ABA(脱落酸)含量的变化情况.结果表明:当花芽生理分化期,ZR(玉米素核苷)、IAA(生长素)、GA(赤霉素)、ABA(脱落酸)的含量迅速下降,到花芽形态分化开始前降到较低水平;花芽形态分化开始后,ZR(玉米素核苷)、IAA(生长素),GA(赤霉素),ABA(脱落酸)的含量呈现出前期上升、中期波动、末期上升的变化规律;花芽生理分化期,ZR/GA、ZR/IAA、ABA/GA和ABA/IAA之值迅速上升,而当花芽形态分化期,各比值在较高水平上的波动大.后期下降.因此,内源激素间的平衡与综合作用促进了花芽的分化. 相似文献
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试验采用室内培养法,研究了不同浓度的硝酸钾(KNO3)、高锰酸钾(KMnO4)、赤霉素(GA3)、聚乙二醇(PEG)对菘蓝种子萌发及幼苗生长的影响,以探讨促进其种子萌发及幼苗生长的有效途径,为菘蓝的规模化栽培及生产提供理论依据。结果表明:与对照相比,2g/L KNO3处理的幼苗根长提高61.35%,10g/L KMnO4处理的幼苗保水力提高81.58%,3种浓度GA3处理幼苗根系活力分别提高17.88%、21.85%、13.82%,0.2g/L GA3处理使种子发芽势和发芽率分别提高38.90%、79.18%,幼苗鲜重和苗长分别提高84.38%、24.24%;3种浓度PEG处理的幼苗根系活力分别提高14.08%、19.32%、13.57%,鲜重分别提高75.00%、106.25%、87.50%,200g/L PEG、300g/L PEG处理的种子发芽率分别提高68.15%、46.93%。综上所述,以GA3溶液处理效果最好,其中,以0.2g/L GA3处理效果最佳,能显著提高种子发芽率及发芽势,促进幼苗生长。 相似文献
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Research on enhancing coning in commercially important conifers(gymnosperms) is reviewed. The prospects for enhancing flowering,for the purposes of breeding and seed supply, appear good, particularlyfor members of the Pinaceae. The most successful techniquesinvolve the application of the gibberellin A4 and A7 mixturecombined with cultural treatments such as drought, root pruningor girdling. There is scope for these techniques to be usedin outdoor seed orchards but more particularly with container-grownplants in polythene houses. Less research has been publishedon enhancing flowering in broadleaves (angiosperms) though therehas been considerable attention to factors affecting blossomand fruit development. Techniques that encourage rapid vegetativegrowth so that plants quickly reach a certain minimum size maybe the most promising to promote flowering of broadleaves. Avenuesfor further research are considered. 相似文献
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Seedlings of three families of jack pine (Pinus banksiana Lamb.) were subjected to 16 combinations of photoperiod, growth environment (outdoors, greenhouse and biotron) and gibberellin (GA(4/7)) treatment. After 14 months, which included two dormancy induction periods, ovulate flowering was observed. There was a strong positive correlation between flowering and seedling height; female flower production was stimulated by both a declining photoperiod during bud development and GA(4/7) treatment; and there was an interaction between GA(4/7) treatment and family, such that the difference in flowering intensity between a late-flowering and an early-flowering family was eliminated by GA(4/7) treatment. The results suggest that the genetic control over the time of onset of flowering, and GA(4/7)-induction of flowering depend on a common mechanism. 相似文献
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Almqvist C 《Tree physiology》2003,23(6):413-418
Effects of varying the time of stem injection of gibberellin A4/7 (GA4/7) on male and female flowering in potted grafts of Pinus sylvestris L. in a greenhouse were monitored in two consecutive years. Flowering results for each application time were correlated with phenological characteristics and abiotic factors in an attempt to find reproducible indicators of the most effective time of GA4/7 treatment. The GA4/7 treatment had a significant effect on female flowering, but no effect on male flowering. In both years, the largest effect on female flowering was obtained with the latest application. Percent shoot and needle elongation (relative to final length) were poor indicators of the optimal time of GA4/7 application. Three heat sum accumulation models gave equally accurate predictions of the optimal time for GA4/7 application; therefore, the simplest model, i.e., the sum of degree-days, is recommended. The timing window for stimulation of female flowering with GA4/7 had a lower limit of about 500 degree-days (threshold +5 degrees C), and the optimal time of GA4/7 application was after about 700 degree-days. 相似文献
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Ornamental plants play an increasingly important role in urban habitats in Turkey. Scale insects are a serious pest group that attack ornamental plants. In this study, a total of 67 species of Coccoidea belonging to 7 families, which are harmful to ornamental plants, are given in Tables 1, 2, and 3. These tables are based on the authors data and on bibliographic sources. The most numerous families are Diaspididae (31), Coccidae (23), Pseudococcidae (7), Eriococcidae (3), and the Margarodidae, Kermesidae and Astereolecaniidae (1). Twenty-one of these species are harmful to flowering fruit and deciduous trees, 5 species are harmful to conifers, while 15 species are important orchard pests. Their hosts and distributions are also taken into consideration. 相似文献
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林木种子园生殖系统研究进展 总被引:5,自引:0,他引:5
本文对种子园生殖系统中亲本开花物候、配子产量、球果和种子败育、授粉机制、球花分布对种子园遗传效率的影响及其研究进展作了综述。论述了开花物候、球花产量与配子贡献以及种子园生殖系统遗传效率的关系。不同亲本开花物候及授粉机制的差异限制了亲本之间的基因交流 ,雌雄球花产量、球果败育及其雌雄球花空间分布的差异都会引起亲本配子贡献的变化 ,并最终影响种子园生殖系统的遗传效率。为提高种子园种子的遗传品质和遗传多样性 ,更系统深入地研究种子园生殖系统是必要的 相似文献
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林木成花分子基础研究进展 总被引:2,自引:1,他引:1
多数林木的幼年期长、始花期晚,为了探索林木早花的分子机制,克服经济林木育种周期长的难题,为进一步的林木童期调控和分子育种研究奠定基础,综述了高等植物的成花假说、成花诱导分子机理、林木成花相关基因的克隆及相关生理信号的研究进展. 相似文献
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赤霉素及多效唑对3种草本花卉花期与株高的影响 总被引:3,自引:0,他引:3
采用不同质量浓度的赤霉素与多效唑对3种草本花卉金盏花、雏菊及三色堇的全株进行了喷施处理,分析了其对3种花卉开花起始时间、花期持续时间及株高的影响,主要研究结论是:(1)合适质量浓度的赤霉素喷施具有促使金盏花、雏菊及三色堇提前开花、缩短花期与增加株高的作用,而合适质量浓度的多效唑喷施正好相反,其具有推迟开花、延长花期与减小株高的作用;(2)在一定的范围内赤霉素的质量浓度与3种花卉花期的长短呈显著或极显著的负相关(p<0.05或p<0.01),而多效唑的质量浓度与3种花卉的花期呈正相关,其中三色堇的花期天数与多效唑质量浓度间的相关程度达极显著水平(p<0.01);(3)100 m g/L的赤霉素喷施可使金盏花、雏菊及三色堇分别提前18.0 d、11.0 d和12.4 d开花,并使其花期持续时间分别缩短3.7%、11.0%和18.3%,结实期株高分别增加86.6%、37.0%和101.6%;(4)100 m g/L的多效唑喷施可使金盏花、雏菊及三色堇分别推迟19.7 d、12.0 d和18.4 d开花,并使其花期持续时间分别延长11.0%、38.4%和31.1%,结实期株高分别减小39.4%、53.3%和41.6%. 相似文献