Comparison of concentrations of copper in plasma and serum from farmed red deer (Cervus elaphus)

AIM: To determine the relationship between the concentrations of Cu in plasma and serum in red deer, and to compare this relationship with those previously reported in cattle and sheep.

METHODS: Paired serum and heparinised plasma samples from 114 red deer from 10 herds (n=6-20 per herd) were analysed for concentrations of Cu. Samples were collected either at slaughter (n=84; eight herds) or by jugular venepuncture (n=30; two herds). Thirty-nine of the samples taken at slaughter were from adult hinds from four herds, while other samples were taken from 10–14-month-old males, except for one herd (10 samples) where an equal number of 8–9-month-old males and females were sampled. The effect of age, gender and herd on the relationship between concentrations of Cu in plasma and serum was assessed using univariate ANOVA. The individual results for concentrations of Cu in serum were compared with those in plasma, using limits-of-agreement plotting.

RESULTS: The mean concentration of Cu in plasma was not significantly different from that of serum (0.048; 95% CI=-0.14 to 0.24 µmol/L). There was no effect of age, sex or herd on this relationship.

CONCLUSIONS: In deer, there was no significant difference between concentrations of Cu in plasma and serum regardless of age, sex or herd of origin.

CLINICAL RELEVANCE: In contrast to the situation in cattle and sheep, the concentration of Cu in serum can be used interchangeably with that in plasma for the estimation of concentration of Cu in blood of red deer.     

Preliminary observations on lungworms in farmed and feral red deer (Cervus elaphus) in Denmark

Deer farming is on its increase in Denmark. Based on experiences from other countries, lungworm infection is expected to cause severe problems in farmed deer. Therefore, in the present paper, which is the first one to deal with pathogens in Danish deer farms, the faecal larval excretion in red deer is examined.     

Dictyocaulus infection in farmed red deer (Cervus elaphus)

Post mortem examination of red deer calves on a deer farm situated on hill ground in north-east Scotland revealed infection by a lungworm morphologically similar to Dictyocaulus viviparus. Trials were conducted to monitor the natural development of D viviparus infection in red deer, to investigate the value of a commercial lungworm vaccine and to evaluate methods of treating clinical cases. The findings indicate that the syndrome may be less apparent in red deer than it is in cattle, protection might be gained by vaccination and that housing and medication provide useful therapy. The extent of clinical disease is likely to depend on the general health, bodily condition and nutritional status of the animals versus the weight of infection acquired from the pasture. However, various factors can affect both sides in this confrontation.     

Parapoxvirus infections in New Zealand farmed red deer (Cervus elaphus)

Outbreaks of infection due to a parapoxvirus were reported on eight New Zealand deer farms. Scabby lesions were seen variably on the muzzle, lips, face, ears and neck of red deer (Cervus elaphus) with morbidity rates reaching 100%. On three farms multifocal lesions were also present on the velvet. Deaths were reported on two properties where the lesions were extensive and secondary bacterial infections had occurred. On one of these farms multifactorial disease was suspected. Poxvirus particles were seen by negative contrast electron microscopy in scab material from all eight properties. Morphologically the deer virus resembled a parapoxvirus, but restriction endonuclease analysis showed its DNA fragment patterns were distinct from those of orf (contagious ecthyma) virus.     

Risk factors for dystocia in farmed red deer (Cervus elaphus).

OBJECTIVE: To analyse data from a study of deer herd health and productivity to identify potential risk factors for the occurrence of dystocia. DESIGN: A 2-year longitudinal observational study of 14 red deer farms was carried out in New Zealand. About 2700 hinds were monitored each year for individual characteristics including body condition score (BCS), and reproductive performance. Grazing and calving management practices were recorded. During calving, farmers recorded calving difficulties (dystocia) that needed human assistance or caused the death of the hind. Risk factors potentially affecting the occurrence of dystocia were identified and analysed by multivariable statistical techniques. RESULTS: The percentage of hinds with dystocia was 0.52% (95% CI 0.32 to 0.78). Hinds with a post-winter BCS over 3.5 were 2.7 times as likely to experience dystocia as hinds scoring 3.5 or less. Given the same BCS class, bodyweight gain in late pregnancy was associated with an increased risk of dystocia. Grazing steep paddocks in the last trimester of pregnancy decreased the risk of dystocia in hinds with a BCS over 3.5. CONCLUSIONS: The risk of dystocia on red deer farms may be reduced if key individual animal, management and environmental factors are optimal.     

Pre-orbital gland opening in farmed red deer (Cervus elaphus) during stressful handling

Stressful manipulation by humans has been previously shown to result in opening the pre-orbital gland of the newborn red deer. We hypothesized that exposure of red deer to stressful handling would result in opening the pre-orbital gland. Further, we tested the potential factors associated with pre-orbital opening, including season, sex, age and struggling behavior. Pre-orbital gland status was observed in 76 red deer (48 males, 28 females) during 281 handling events with the animal isolated and fixed in a mechanical restraint cradle (crush) within 3 consecutive years. The deer age ranged from 1 to 5 yr in males, and from 1 to 11 yr in females at the beginning of observations. The proportion of handling events with an open gland was considerably greater during than before handling (27.76 vs. 0%, respectively, P < 0.001). The deer that struggled or stiffened revealed 2-fold greater incidence of pre-orbital opening (P < 0.01), regardless of sex. Thus, pre-orbital opening seemed to accompany the behaviors that have been found to be related to acute stress. The probability that the animal opens its pre-orbitals during handling sharply increased at the beginning of the rut (mating season, P < 0.01). Deer that threatened the handler (10% out of 215 observations) opened their pre-orbitals 30 times more often than nonthreatening ones. These effects indicate that other motivations are likely involved in the opening of pre-orbitals, in addition to the perception of a stressful event. Incidence of pre-orbital opening decreased with age in both sexes (P < 0.05) across 3 consecutive yr of observations, which suggests habituation to repeated handling. Sex of the animal did not influence the probability of pre-orbital opening. We found significant variability in pre-orbital opening across the individuals (P < 0.001). In conclusion, we confirmed an association between opening of the pre-orbital gland and stressful handling in sub-adult and adult red deer, although repeated human handling did not elicit such incidence of pre-orbital opening as found in newborn red deer calves. Our results support multifactorial origins of pre-orbital opening and prompt the necessity of further research to distinguish between different motivations that might have been involved in opening of pre-orbitals.     

Osteochondrosis, skeletal abnormalities and enzootic ataxia associated with copper deficiency in a farmed red deer (Cervus elaphus) herd

A red deer herd of 150 mixed-age hinds, 48 stags and 102 weaners was identified as severely copper deficient during an observational study of 15 deer farms in the lower North Island of New Zealand during 1992 and 1993. Severe lameness was observed in nine weaners in 1992 (8.8% prevalence) and 15 in 1993 (12% prevalence). Typical abnormalities included swollen hocks and carpal joints and outward rotation of hind legs with hocks touching. At postmortem examination, there were epiphyseal fractures of the femoral head, severe degenerative arthropathy of the coxo-femoral joints and erosions of cartilage in many other limb joints. Osteochondrosis was confirmed histologically. Concurrently, three adult hinds and one adult stag developed into-ordination typical of enzootic ataxia which was confirmed histologically. Blood and liver copper concentrations in untreated affected weaner deer ranged from undetectable to 16.0 micromol/l (mean 7.6 micromol/l) and 25 to 53 micromol/kg (mean 39 micromol/kg), respectively. Mean blood copper concentrations in unaffected weaners in March 1992 and 1993 were 5.3 micromol/l and 4.4 micromol/l, respectively. The mean blood copper concentration in seven hinds in September 1992, prior to onset of clinical signs of enzootic ataxia, was 1.5 micromol/l (range 1.0-2.4 micromol/l). At other times of the year, mean blood copper concentrations ranged up to 12.5 micromol/l in adults and 8.9 micromol/l in weaners before treatment began in 1993. Pasture analyses showed copper contents of 6-11 ppm in 11 samples collected during 1992 and 1993. Sulphur ranged from 0.18 to 0.37%, molybdenum from 0.51 to 3.56 ppm and iron from 130 to 2886 ppm. These measurements supported a diagnosis of secondary copper deficiency. Supplementation with oral oxidised copper wire particles was undertaken from December 1992, resulting in an improvement in blood copper concentrations in some classes of deer. No further clinical abnormalities have been observed.     

Potential wildlife sources of Yersinia pseudotuberculosis for farmed red deer (Cervus elaphus)

During 1982 and 1983 15 serotype I,6 serotype II, 1 serotype III and 3 untyped strains of Yersinia pseudotuberculosis were isolated from 675 apparently normal small mammals and birds from the Invermay farm and nearby rubbish tip with the following prevalence rates: feral cats 27.8%, Norway rats 8.6%, mice 5.5%, hares 3.8%, rabbits 1.9%, ducks 5.3%, sparrows 2.3%, seagulls 2.3% and starlings 1.7%. For rabbits a significantly higher prevalence of infection was found in the autumn/winter period (4.8%) than the spring/summer period (0%).

Insufficient numbers of other mammals were obtained to demonstrate any seasonal difference in prevalence. All bird isolations were obtained between March and July (8/158) compared with none from August to October (0/144). It appears that a number of free-living species of small mammal and birds may be reservoir hosts for Y. pseudotuberculosis and potential sources of infection for red deer on the Invermay farm.     

Absence of a vitamin B12 weight gain response in two trials with growing red deer (Cervus elaphus)

Abstract

Extract

Madam:- Cobalt deficiency has long been recognised in New Zealand as a disease in sheep and cattle which may occur when they graze pastures of low cobalt content <0.08 mg/kg DM and >0.04 mg/kg DM respectively. (2 Clark, R.G. and Millar, K.R. 1983. “Cobalt”. In The Mineral Requirements of Grazing Ruminants, Edited by: Grace, N.D. 27–37. NZ Society of Animal Production. Occasional publication No. 9 [Google Scholar]) In recent years the farming of red deer (Cervus elaphus) on soil types known to be marginally, moderately or severely deficient(1 Andrews, E.D. 1971. Cobalt deficiency in sheep and cattle, New Zealand Department of Agriculture. Bulletin 180 [Google Scholar]) has raised the question of how susceptible these species are to cobalt deficiency. To the authors' knowledge there is no published information on the susceptibility of red deer to cobalt deficiency.     

Experimental tuberculosis in red deer (Cervus elaphus)

This study was designed to investigate experimental Mycobacterium bovis infection of red deer (Cervus elaphus). Three intravenously inoculated deer (dose 10µg–1000µg) developed miliary tuberculosis of the lungs and all died within 28 days of being infected. No clinical illnesses were observed in four subcutaneously (dose 1 uµg–100uµg) and three intratracheally (dose lµg–100µg) inoculated deer. At the conclusion of the experiment six weeks post inoculation, these seven animals reacted to 2 mg/ml of bovine purified protein derivative. The principal lesions in the intravenously inoculated deer were in the lungs which had multiple foci of necrosis containing very large numbers of acid fast bacilli. A gradation of changes was seen in the subcutaneously inoculated deer. The animal receiving the 1µg dose only had lesions at the injection site and the draining prescapular lymph node. Deer receiving higher doses also had histopathological changes in the lungs and liver. Microscopic changes in the intratracheally infected animals were restricted to the thoracic cavity. The ability of the deer to control infection was related to the route of inoculation.     

Experimental tuberculosis in red deer (Cervus elaphus)

This study was designed to investigate experimental Mycobacterium bovis infection of red deer (Cervus elaphus). Three intravenously inoculated deer (dose 10 microg-1000 microg) developed miliary tuberculosis of the lungs and all died within 28 days of being infected. No clinical illnesses were observed in four subcutaneously (dose 1 microg-1000 microg) and three intratracheally (dose 10 microg-100 microg) inoculated deer. At the conclusion of the experiment six weeks post inoculation, these seven animals reacted to 2 mg/ml of bovine purified protein derivative. The principal lesions in the intravenously inoculated deer were in the lungs which had multiple foci of necrosis containing very large numbers of acid fast bacilli. A gradation of changes was seen in the subcutaneously inoculated deer. The animal receiving the 1 microg dose only had lesions at the injection site and the draining prescapular lymph node. Deer receiving higher doses also had histopathological changes in the lungs and liver. Microscopic changes in the intratracheally infected animals were restricted to the thoracic cavity. The ability of the deer to controlled infection was related to the route of inoculation.     

Tuberculosis in imported red deer (Cervus elaphus)

An outbreak of tuberculosis due to Mycobacterium bovis in farmed red deer imported from an eastern European country is described. Twenty-six of the 106 deer examined at autopsy were found to be infected and 19 had visible lesions of tuberculosis. Single comparative intradermal tuberculin tests on 51 deer showed that the test had a specificity of 61.3 per cent and a sensitivity of 80 per cent relative to subsequent biological and cultural tests on tissues taken at autopsy. Three hundred and seventy eight culled fallow and sika deer which had been running in a park in contact with some of the infected animals were found to be free of tuberculosis.     

Blood constituents of farmed red deer (Cervus elaphus): I. Haematological values

Approximately 100 farmed male and female red deer aged three months and over were blood-sampled and haematological parameters were measured. The deer were sampled by jugular venepuncture without tranquilization. Mean values were haemoglobin (16.0 g/dl), packed cell volume (44.6%), mean corpuscular haemoglobin concentration (35.8 g/dl), total plasma protein (66.0 g/l). fibrinogen (4.8 g/l) and white blood-cell numbers (5.80 x 10(9)/l). Differential white blood-cell counts were neutrophils (53.9%), eosinophils (4.8%), basophils (3.0%), lymphocytes (37.2%) and monocytes (1.1%). There were no significant differences in any parameter measured between sexes or between age groups three to eight months, nine to eighteen months, or older.     

Blood constituents of farmed red deer (Cervus elaphus): I. Haematological values

Approximately 100 farmed male and female red deer aged three months and over were blood-sampled and haematological parameters were measured. The deer were sampled by jugular venepuncture without tranquillization. Mean values were haemoglobin (16.0g/dl), packed cell volume (44.6%), mean corpuscular haemoglobin concentration (35.8g/dl), total plasma protein (66.0g/l). fibrinogen (4.8g/l) and white blood-cell numbers (5.80 x 10⁹/l). Differential white blood-cell counts were neutrophils (53.9%), eosinophils (4.8%), basophils (3.0%), lymphocytes (37.2%) and monocytes (1.1%). There were no significant differences in any parameter measured between sexes or between age groups three to eight months, nine to eighteen months, or older.