Short term response of soil microinvertebrates to application of entomopathogenic nematode-infected insects in two tillage systems

Entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae occur naturally in the soil and are produced commercially for the management of soil-dwelling pests. EPN infected cadavers also represent a potential resource for other soil organisms. We examined the short-term (24 h) response in abundance, diversity and community composition of localized soil microinvertebrates to the presence of EPN-infected insect cadavers in no-till and conventional-till maize. We hypothesized that the response of soil microinvertebrates to the EPN-infected cadavers would vary by soil management practices and EPN species. We expected to observe greater numbers and diversity of arthropods in no-till compared with conventional-till soil, and in the vicinity of steinernematid-infected insect cadavers compare to what would be found in the vicinity of heterorhabditid-infected cadavers. 45,606 invertebrates were collected and identified to 134 morphotaxa. Tillage regime accounted for the majority of the variation observed (84.6%), whereas nematode treatment accounted for 7.5%. Taxonomic richness of invertebrates was greater in treatments with Steinernema carpocapsae-infected cadavers than with Heterorhabditis bacteriophora-infected cadavers. Some invertebrates increased in abundance where EPN were applied whereas others decreased, regardless of tillage practice. Applications of Galleria cadavers infected with steinernematids elicited positive responses from two mite taxa, Galumnidae and Scheloribates spp., while negative responses were elicited from three mite (Histiostomatidae, Scheloribates spp., Eupodes spp.), taxa and Entomobryidae (Collembola) in response to applications of Heterorhabditis-infected cadavers.     

Reducing the dimensionality of soil microinvertebrate community datasets using Indicator Species Analysis: Implications for ecosystem monitoring and soil management

Soil microinvertebrates are closely associated with soil decomposition and nutrient cycles and may be particularly responsive indicators for soil management practices. However, identification of appropriate bioindicator species for many systems has been severely limited by a lack of information on species taxonomy, distribution, and functional role. We evaluated Indicator Species Analysis (ISA) as an objective method for assessing the indicator potential of different taxa without regard to their ecological role or expected management response. Restricting ordination and site classification to significant indicator morphotaxa reduced the dimensionality of the community data matrix by 69% while only slightly decreasing the efficiency of unsupervised classification (from 87.2 to 84.4%); the percentage of total variability explained by first two PCA axes increased following ISA. When these same indicator morphotaxa were used to classify an independent set of samples, the percentage of total variability explained by the first two PCA axes increased from 55.3 to 65.2%; cluster analysis of the test dataset correctly classified 47 out of 50 plots by cover type (94% accuracy). However, restriction of analysis to indicator morphotaxa alone reduced detection of differences between sampling dates relative to the complete dataset. Although care needs to be taken to ensure that the dataset used for indicator selection is fully representative of underlying temporal and spatial variability, ISA appears to overcome many of the limitations associated with parametric and multivariate approaches for identifying indicator morphotaxa and has the potential to greatly reduce the taxonomic expertise and labor costs associated with sorting and identification of soil microarthropods.     

Effect of seasonality and agricultural practices on occurrence of entomopathogenic nematodes and soil characteristics in La Rioja (Northern Spain)

The effects of seasonality and land management on entomopathogenic nematodes (EPNs) distribution and on soil characteristics were studied in natural areas and agricultural fields under organic and conventional management in La Rioja (Northern Spain). The population density of the native EPNs as well as air temperature, rainfall, soil fertility, soil moisture, and soil content of heavy metals were seasonally recorded over 2 consecutive years. The highest occurrence of EPNs was observed in autumn, followed by spring and summer. Agricultural management influenced the amount of soil organic matter, nutrients and heavy metals, as well as soil volumetric moisture and temperature, leading to statistical differences mainly between natural and agricultural sites, but also in some cases between different types of agricultural management. The usefulness of soil organic matter, C, N, P and K content, as well as the C/N, C/P and N/P ratios as indicators for differentiating conventional and organic crop management as well as the role of agricultural practices such as tillage and fertilizing is discussed.     

Soil microarthropods are only weakly impacted after 13 years of repeated drought treatment in wet and dry heathland soils

Studies of biological responses in the terrestrial environment to rapid changes in climate have mostly been concerned with aboveground biota, whereas less is known of belowground organisms. The present study focuses on mites and springtails of heathland ecosystems and how the microarthropod community has responded to simulated climate change in a long-term field experiment. Increased temperature and repeated drought was applied for 13 years to field plots located in Wales, The Netherlands and Denmark representing sites of contrasting climatic conditions with respect to precipitation and temperature. This approach provided an opportunity to study biological responses on a local (within sites) and regional scale. Warming treatments increasing night time temperature (0.3–1 °C higher than ambient at 5 cm soil depth) had no detectable effects on the microarthropod communities. Increased intensity and frequency of drought had only weak persistent effects on springtail species composition, but practically no effect on major mite groups (Oribatida, Prostigmata or Mesostigmata) suggesting that ecosystem functions of microarthropods may only be transiently impacted by repeated spring or summer drought.     

Diversity surfaces and species wave fronts in a soil microarthropod assemblage: Adding the dimension of time

As a general rule, animal species of intermediate size within a given taxonomic group are most abundant in nature. It is not known if these patterns occur in small-bodied taxa, such as soil microarthropods, or how these patterns change through time. Here I show that Oribatida (Acari), the most abundant and diverse arthropod fauna of coniferous forest soils, exhibit this pattern. However, the pattern is more complex than reported for other arthropods. I analyzed the total species surface comprising 6613 individuals and 54 species by forest stand. The underlying pattern consists of 15-year stands and 30-year stands forming two distinct and separated maxima. These results suggest that assemblage patterns form early in the development of ecological communities, and that these patterns appear within the soil assemblage as waves propagating in species–abundance–body size space during forest development. These results also support the assertion that undescribed species will likely be of intermediate size within a group. This analysis contributes to investigations of biodiversity and body size relationships by adding the temporal dimension. Potential applications are in disturbance and indicator studies or other work where changes in assemblage structure are used as measures of disturbance or as response variables in manipulative studies.     

Nematode faunal response to long-term application of nitrogen fertilizer and organic manure in Northeast China

Nematode faunal response to the long-term (20-year) application of nitrogen fertilizer and organic manure was monitored in a Hapli-Udic Cambosol of Northeast China, where no fertilizer (C), nitrogen fertilizer (N), organic manure (M) and nitrogen fertilizer plus organic manure (N + M) treatments were compared. The obtained results showed that total nematode abundance responded positively to the M and N + M treatments. The numbers of Acrobeloides increased in the M and N + M treatments at maize jointing and booting stages, and those of Aphelenchoides were higher in the N + M treatment than in the N treatment at maize ripening stage. Early in the growing season, the numbers of bacterivores of cp-1 (cp, colonizer-persister) and cp-2 guilds were higher in the M and N + M treatments than in the N treatment, while those of bacterivores and fungivores of cp-4 guilds were higher in the C treatment than in the N, M and N + M treatments. Except at maize seedling stage, the numbers of fungivores of cp-2 and herbivores of cp-3 guilds were higher in the N + M treatment than in the C treatment. Ratios of the weighted abundance of representatives of specific functional guilds were used as indicators of food web structure, enrichment and decomposition channels. Values of the enrichment index (EI) at maize seedling and jointing stages were higher in the M and N + M treatments than in the C and N treatments, while an opposite trend was observed in the channel index (CI). Higher EI and lower CI suggest an enriched soil food web dominated with bacterial decomposition channels in the M and N + M treatments.     

Residue incorporation and N fertilization affect the response of soil nematodes to the elevated CO2 in a Chinese wheat field

The interplay between the carbon and other nutrient cycles is the key to understand the responses of soil ecosystems to climatic change. Using the free-air CO₂ enrichment (FACE) techniques, we carried out a multifactorial experiment in a Chinese rice-wheat rotation system, to investigate the response of soil nematodes to elevated CO₂ under different application rates of N fertilizer (225.0 kg N ha⁻¹ (HN) and 112.5 kg N ha⁻¹(LN), respectively) and residue incorporation (0 kg C ha⁻¹ (ZR), 1000 kg C ha⁻¹ (MR) and 2000 kg C ha⁻¹ (HR), respectively). This study was conducted during the wheat growing season of 2007 after expose to the elevated CO₂ for three years. The results in our study indicated that seasonality is an important factor in determining changes in the nematode abundance and diversity. The residue addition effects were more obvious than the elevated CO₂, which significantly influenced the abundance of total nematodes and plant-parasites, and some ecological indices. The interactions between residue addition and CO₂ significantly influenced nematode dominance and structure indices. High level of N fertilization was found to decrease the nematode diversity, generic richness and maturity indices at wheat jointing stage. There are significant interactions between N fertilization and elevated CO₂ for abundance of total nematodes and different trophic groups.     

Soil properties and habitats determine the response of bacterial communities to agricultural wastewater irrigation

Increasing temperatures and variability of precipitation events due to climate change will lead in the future to higher irrigation demands in agroecosystems.However,the use of secondary treated wasterwater(TWW)could have consequences for the receiving soil environment and its resident microbial communities.The objective of this study was to characterize the importance of soil properties and habitats to the response of soil bacteria and archaea to irrigation with TWW.Two agricultural soils with contrasting textures(loamy sand or silt loam)and,for each,three variants differing in soil organic carbon and nitrogen,as generated by long-term fertilization,were analyzed.For each of these six soils,prokaryotic communities from two habitats,i.e.,root-free bulk soil and the rhizosphere of developing cucumber plants in the greenhouse,were characterized.Communities were analyzed by the quantity and diversity of their polymerase chain reaction(PCR)-amplified 16S rRNA genes.To account for TWW-associated nutrient effects,potable water(PW)served as a control.Amplicon sequence analysis showed that prokaryotic communities mainly consisted of bacteria(99.8%).Upon irrigation,regardless of the water quality,prokaryotic diversity declined,p H increased,and no bacterial growth was detected in bulk soil.In contrast,the growth of cucumbers was stimulated by TWW,indicating that plants were the main beneficiaries.Moreover,strong responses were seen in the rhizosphere,suggesting an indirect effect of TWW by altered rhizodepositions.The main bacterial responders to TWW were Proteobacteria,Bacteroidetes,Actinobacteria,and Planctomycetes.Changes in bacterial communities due to TWW were less pronounced in all variants of the silt loam,indicating the importance of clay and soil organic carbon for buffering effects of TWW on soil bacterial communities.Hence,soil organic carbon and soil texture are important parameters that need to be considered when applying TWW in agriculture.     

Palatability of microfungi to soil arthropods in relation to the functioning of arbuscular mycorrhizae

We investigated the feeding preferences of six species of mites and collembolans for three fungi commonly associated with roots of Acer saccharum (Glomus macrocarpum, Alternaria alternata and Trichoderma harzianum), from a maple-forest soil in southern Ontario, Canada. Experiments were also conducted in vitro to determine animal feeding responses to (1) increasing quantities of hyphal biomass, (2) the presence of root vs. litter fungal substrates, and (3) hyphae of different widths of Glomus macrocarpum. The results indicate that arthropods prefer to graze in the litter region rather than in the deeper soil layers. Under ideal moisture/temperature conditions, animals are forced to the lower regions by interspecific interactions. They prefer to graze on hyphae of conidial fungi rather than on those of arbuscular mycorrhizal fungi. When arbuscular mycorrhizal fungal hyphae are grazed, there is a clear preference for the narrower hyphae, which are those further away from the root. The thicker hyphal segments, commonly found connecting absorptive hyphal fans to roots, were less preferred. These data are not consistent with the hypothesis that microarthropods are detrimental to arbuscular mycorrhizal associations, and suggest that Glomalean fungi may have evolved mechanisms to deter grazing by microarthropods.     

Effect of phosphogypsum application and bacteria co-inoculation on biochemical properties and nutrient availability to maize plants in a saline soil

Phosphogypsum (PG), which contains Ca, P and S and has an acidic effect, may be applied to manage soil constraints such as alkalinity and salinity. For increasing nutrients bioavailability, biofertilizers are commonly applied. Therefore, the aim of this study was to assess PG effect either alone or in combination with the mixed co-inoculation of plant growth promoting rhizobacteria on a saline soil. In a greenhouse pot experiment with maize (Zea mays L.), the inoculated and non-inoculated saline soils were treated with PG at 10 g kg^?1 (PG10), 30 g kg^?1 (PG30), and 50 g kg^?1 (PG50). The soil pH, electrical conductivity (EC_e), and macro-(NPK) and micronutrients (Fe, Mn, Zn, and Cu) availability to mays were examined. Applying PG reduced soil pH and co-inoculation induced significant decreases in soil EC_e. Applying PG increased significantly soil available P. Applying PG combined with co-inoculation effectively increased the soil available K. The soil available micronutrients decreased significantly with PG. However, the inoculated maize treated with PG showed significant higher dry weight (82.1–127.4%) and nutrients uptake than the control. It could be concluded that PG along with co-inoculation may be an important approach for alleviating negative effects of salinity on plant growth.     

Soil carbon sequestration and crop yield in response to application of chemical fertilizer combined with cattle manure to an artificially eroded Phaeozem

Organic manure application is a feasible approach to alleviate the deterioration of soil erosion on soil organic carbon (SOC). However, to what extent manure application can restore carbon contents in SOC fractions in the eroded Phaeozems remains unknown. A 5-year field experiment was conducted in an artificially eroded Phaeozem with up to 30 cm of topsoil being removed. Chemical fertiliser, or chemical fertiliser plus cattle manure was applied. The contents of SOC were 23.6, 21.6 and 15.1 g C kg^?1 soil for non-soil removal control, 10 and 30 cm of topsoil removal, respectively. Compared with the chemical fertiliser-only treatment, the chemical fertiliser plus manure application markedly increased SOC contents by 30–45% and C sequestration rates by 7.1–9.0-fold, especially in the fraction of 53–250 μm particulate organic carbon. However, with manure applied, SOC content in the fraction of mineral associated organic carbon in the 30 cm topsoil-removed soil was 2.9 g kg^?1, 14.7% less than control (3.4 g kg^?1). The combination of chemical fertliser and manure application effectively restored SOC in the eroded Phaeozems mainly through increasing the size of 53–250 μm particulate organic C fraction, but did not improve the SOC stability in severely eroded Phaeozems.     

塔克拉玛干沙漠地表浅层土壤热扩散率、温度和热通量计算方法的比较研究

利用塔克拉玛干沙漠大气环境观测站2008年7月30日至8月4日的土壤温度、湿度和土壤热通量观测资料,定量分析了沙漠土壤热扩散率、温度、热通量的变化规律。采用谐波法、振幅法、相位法和热传导对流法分别计算了5~20 cm沙漠土壤的热扩散率,在此基础上,以5 cm深度的土壤层为上边界条件,计算了10和20 cm深度的土壤温度和8 cm深度的土壤热通量。结果表明:谐波法计算沙漠土壤温度的精度最高,10和20 cm深度的土壤温度计算值与观测值的标准误差分别为0.167℃和0.127℃;热传导对流法对土壤温度的计算结果好于振幅法和相位法。同样,谐波法计算的沙漠土壤热通量值与观测值的误差最小,计算值与观测值的相关系数R2达到0.976,热传导对流法次之,振幅法和相位法的误差最大。     

Effect of fertilizer application based on soil test crop response approach on active and passive pools of soil organic matter in maize–wheat cropping system

ABSTRACT

A long-term fertilizer experiment was initiated in kharif 2007 in a maize–wheat sequence at the Experimental Farm of Department of Soil Science, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur under the network of All India Coordinated Research Project on Soil Test Crop Response (STCR) Correlation Studies. The experiment consisted of eight treatments (control, general recommended dose, soil test based, farmers’ practice, target yield of 25 (rabi)/30 (kharif) and 35 (rabi)/40 (kharif) q ha^?1 either with or without 5 t ha^?1 farm yard manure) replicated thrice in a randomized block design. All the passive and active pools of soil organic matter (SOM) were highest under STCR treatments for target yield 35 q ha^?1 + farmyard manure (FYM). The highest value of both humic and fulvic acids under STCR treatment for target yield 35 q ha^?1 with FYM was statistically at par with treatment for target yield 25 q ha^?1 with FYM. The R² value indicated that about 96% to 98% of the total variation in grain yield was attributable to SOM fractions. The contribution of all fractions of SOM towards the total variation in straw yield was 96% to 97%.     

色季拉山4种林型土壤呼吸及其影响因子

土壤碳是森林生态系统最大的碳库,是其森林生态系统碳循环的极其重要组分。森林土壤呼吸时陆地生态系统土壤呼吸的重要组成部分,其动态变化对全球碳平衡有着重要的影响,然而目前对藏东南地区森林土壤呼吸的研究还比较薄弱。为探讨不同林型土壤呼吸差异及其影响因子,采用Li-8100便携式土壤呼吸测定仪,研究了藏东南色季拉山4种原始森林生态系统(高山灌丛AS、方枝柏SS、杜鹃RF、急尖长苞冷杉AGSF)的土壤碳动态。结果表明:(1)藏东南色季拉山寒温带森林土壤呼吸具有明显的日变化和季节变化。在日变化方面,CO2的排放通量存在明显的日变化规律,排放通量在白天16:00左右最高,最低值出现在凌晨6:00左右,一天内土壤呼吸作用均呈单峰型曲线变化。季节变化方面,CO2排放的通量的季节变化趋势表现为6月份随着天气转暖和植被生长土壤呼吸作用逐渐增大,7月份气温最高时土壤呼吸作用也达到最大值随后,9月份气温逐渐下降,土壤呼吸作用也逐渐降低。(2)4种森林类型的土壤呼吸速率在植物生长季内与土壤表层(10 cm)土壤温度均呈不同程度的正相关,而与土壤含水量的相关性较弱。土壤温度是决定藏东南色季拉山土壤呼季节变化的主要因子。该研究为明确森林生态系统土壤呼吸变化规律及其影响因素的控制提供参考,同时对估算地区碳平衡、评估区域碳源汇具有重要意义。     

华北典型区域土壤耕作方式对土壤特性和作物产量的影响

华北平原是我国重要的小麦玉米种植区,长期土壤旋耕免耕和秸秆全量还田带来耕层变浅、犁底层变厚和上移、土壤养分表聚等现象,通过耕作方式改变,解决上述问题对维持区域粮食生产有重要意义。试验以冬小麦-夏玉米轮作系统为研究对象,分别在代表华北平原高产区的栾城试验区和代表中低产区的南皮试验区进行,设置冬小麦播种前进行土壤深耕、深松、窄深松3种处理,以生产上常用的旋耕为对照。所有处理夏玉米季均采用土壤免耕播种,测定项目包括土壤容重、作物根系、作物产量和水分利用效率。结果表明,不同耕作方式对土壤特性和作物产量的影响具有区域差异。南皮试验区土壤深耕(松)显著地(P0.05)提高了作物产量,深耕、深松和窄深松处理的冬小麦产量比旋耕分别增加16.5%、19.3%和13.1%,夏玉米产量分别增加17.3%、16.2%和21.9%,周年产量分别增加16.9%、17.6%和17.8%;深耕、深松和窄深松处理间作物产量差异不显著。栾城试验区冬小麦、夏玉米产量和周年产量各处理之间差异不显著。土壤深耕、深松、窄深松和旋耕均能降低0～20 cm土层土壤紧实度和土壤容重。冬小麦播种后,与土壤耕作前比较,土壤深耕、深松和旋耕处理土壤紧实度南皮试验区分别平均降低71.6%和68.2%,栾城试验区分别降低88.8%和?7.7%,常用的旋耕模式在栾城试区没有降低土壤紧实度。小麦收获时不同耕作方式0～40cm土层的土壤容重均低于土壤耕作前的土壤容重,至夏玉米收获时不同耕作处理的土壤容重与耕作前基本一致,不同耕作处理对土壤容重的影响差异不显著。在南皮试验区, 3种耕作方式与旋耕相比,均显著提高了冬小麦和夏玉米水分利用效率;在栾城试验区,各处理冬小麦和夏玉米水分利用效率差异不显著。本研究结果显示在华北平原高产区连续实施土壤旋耕模式没有影响作物产量,而在中低产区实施土壤深耕或者深松模式更利于作物产量提高。     

Specific response of fungal and bacterial residues to one-season tillage and repeated slurry application in a permanent grassland soil

The dynamics of fungal and bacterial residues to a one-season tillage event in combination with manure application in a grassland soil are unknown. The objectives of this study were (1) to assess the effects of one-season tillage event in two field trials on the stocks of microbial biomass, fungal biomass, microbial residues, soil organic C (SOC) and total N in comparison with permanent grassland; (2) to determine the effects of repeated manure application to restore negative tillage effects on soil microbial biomass and residues. One trial was started 2 years before sampling and the other 5 years before sampling. Mouldboard ploughing decreased the stocks of SOC, total N, microbial biomass C, and microbial residues (muramic acid and glucosamine), but increased those of the fungal biomarker ergosterol in both trials. Slurry application increased stocks of SOC and total N only in the short-term, whereas the stocks of microbial biomass C, ergosterol and microbial residues were generally increased in both trials, especially in combination with tillage. The ergosterol to microbial biomass C ratio was increased by tillage, and decreased by slurry application in both trials. The fungal C to bacterial C ratio was generally decreased by these two treatments. The metabolic quotient qCO₂ showed a significant negative linear relationship with the microbial biomass C to SOC ratio and a significant positive relationship with the soil C/N ratio. The ergosterol to microbial biomass C ratio revealed a significant positive linear relationship with the fungal C to bacterial C ratio, but a negative one with the SOC content. Our results suggest that slurry application in grassland soil may promote SOC storage without increasing the role of saprotrophic fungi in soil organic matter dynamics relative to that of bacteria.     

Soil biota and soil properties in the surface rooting zone of mesquite (Prosopis glandulosa) in historical and recently desertified Chihuahuan Desert habitats

Summary The woody legume, mesquite (Prosopis glandulosa) has expanded from its historical habitats (playas and arroyos) to recently occupied grassland and dune habitats during the desertification of perennial grasslands in the Chihuahuan Desert. We studied historical and recently occupied sites, having hypothesized that the trophic structure and population density of soil microarthropods and nematodes associated with the surface root system of mesquite would differ in sites representing historical and recent habitats, and that the N mineralization potential would be lower in the recent habitats. Our results showed that net N mineralization potential did not differ significantly among the sites, even though soil nutrient concentrations and texture varied widely. Concentrations of organic C, N, and P were lowest in the recent dune habitat and highest at the playa. Very low concentrations of P in the dune and grassland soils implicated P as a limiting factor in these systems. The bacterial-feeding and omnivore-predator functional groups made up the largest fraction of the nematode community at most of the sites. The high density of plant-feeding nematodes at the playa indicated that herbivory is potentially most important at this site. Total microarthropod densities did not vary significantly among habitats, with Collembola densities highest in the mesquite dunes. Grazers were the dominant microarthropod functional group. While both C and N pool sizes were higher in the historical habitats, a higher substrate lability in the recent habitats appeared to support biota populations and N mineralization rates equivalent to those in the playa and arroyo. Differences in soil properties and biota among historical and recent mesquite habitats may be important for understanding the changes that have occurred in Chihuahuan Desert ecosystems during desertification.     

Barley yield response to soil organic matter and texture in the Pampas of Argentina

Soil organic matter (SOM) is known to play a major role in soil fertility due to its influence on physical, chemical and biological properties of soil; and it is closely related to particle size distribution. The ratio of SOM (g kg⁻¹) to clay + silt content (g kg⁻¹) was evaluated as an indicator of soil quality for barley (Hordeum vulgare) grain yield, reflecting N availability and soil physical conditions to which crop development is sensitive. Thirty-eight sites in the semiarid Pampa region of Argentina with a wide range of SOM and texture were evaluated for malting barley yield during three growing seasons. In control plots, 51% of grain yield could be explained by this indicator. The threshold value between high and low N-fertilization response was 4.4. Better yield prediction to almost 68% was achieved by combining the SOM to clay + silt indicator with initial nitrate content of the soil at seeding. This combined indicator was also able to explain a high proportion of water use efficiency, particularly in the early growth stages. The ratio of SOM to clay + silt content provided a better tool for estimating grain yield than nutrient availability or SOM alone.     

Soil organic carbon and nitrogen in aggregates in response to over seven decades of farmyard manure application

The study aimed to evaluate the effects of long-term fertilisation on soil aggregation and the associated changes in soil organic carbon (SOC) and nitrogen (N) pools in aggregates. The combined application of mineral fertiliser and manure improved soil aggregation, SOC and N content in aggregates, compared to manure or mineral fertiliser alone, and thus proved to be a suitable fertilisation strategy to increase C sequestration in agroecosystems.     

黄土高原黑垆土施肥的作物累积产量及土壤肥力贡献

施肥的作物产量和土壤肥力贡献是评价施肥是否科学的关键参数,为了给黄土高原黑垆土区施肥及农业可持续发展提供科学的决策依据,通过设置在黄土高原黑垆上的长期定位试验系统研究了不同施肥的作物产量和土壤肥力贡献。试验设不施肥(CK)、单施氮肥(N)、氮磷配施(NP)、秸秆与氮磷配施(SNP)、单施有机肥(M)和有机肥与氮磷配施(MNP)6个处理。结果表明,所有处理玉米和小麦累积产量与种植年限显著正相关。MNP、SNP、NP、M4处理玉米年均产量分别为8 856、7 089、7 226、7 043 kg·hm~(-2),小麦年均产量分别为4 916、3 747、4 132、3868kg·hm~(-2),均显著高于对照(CK)。SNP、M和MNP处理玉米和小麦产量与NP处理相近或高于NP处理。氮肥、磷肥的作物累积产量贡献量与种植年限分别呈二次凹函数和凸函数关系。氮磷配施(NP)、秸秆还田的作物累积产量贡献量则与种植年限显著正相关,其年均玉米产量贡献量分别为3408、2191kg·hm~(-2),小麦产量贡献量分别为429、206kg·hm~(-2)。长期施用有机肥及秸秆还田可显著增加土壤有机质和全氮含量。长期施磷肥土壤有效磷含量显著增加,而长期施用有机肥土壤有效磷和速效钾含量均显著增加。总体而言,黄土高原黑垆土区长期平衡施用化肥、施用有机肥、秸秆与化肥配合施用及有机肥与化肥配合施用均可增加作物产量,提升土壤肥力质量,适宜化肥施用量为N 90kg·hm~(-2)、P_2O_5 75 kg·hm~(-2),秸秆还田可隔年施磷。