Internally generated reactivation of single neurons in human hippocampus during free recall

The emergence of memory, a trace of things past, into human consciousness is one of the greatest mysteries of the human mind. Whereas the neuronal basis of recognition memory can be probed experimentally in human and nonhuman primates, the study of free recall requires that the mind declare the occurrence of a recalled memory (an event intrinsic to the organism and invisible to an observer). Here, we report the activity of single neurons in the human hippocampus and surrounding areas when subjects first view cinematic episodes consisting of audiovisual sequences and again later when they freely recall these episodes. A subset of these neurons exhibited selective firing, which often persisted throughout and following specific episodes for as long as 12 seconds. Verbal reports of memories of these specific episodes at the time of free recall were preceded by selective reactivation of the same hippocampal and entorhinal cortex neurons. We suggest that this reactivation is an internally generated neuronal correlate for the subjective experience of spontaneous emergence of human recollection.     

Tones and numbers: specificity of interference in immediate memory

Recognition of the pitch of a tone was severely disrupted by the incorporation of six other tones during a 5-second retention interval, even though the intervening tones could be ignored. However, the requirement to recall six numbers spoken at equal loudness during the identical retention interval produced only a minimum decrement in the same pitch-recognition task. Further, the requirement to remember the tone produced no decrement in recall of the numbers. It is concluded that immediate memory for pitch is subject to a large interference effect which is highly specific in nature and which is not due to some limitation in general short-term memory capacity or to a distraction of attention.     

New routes to early memories

Stimulation of one side of the olfactory system during training with odor-milk pairings in neonatal rats results in their ability to recall an odor memory by using the trained but not the untrained side of the brain. In 12-day-old rats, olfactory learning can be recalled by stimulation of either the trained or untrained side. The development of bilateral recall reflects the maturation of olfactory commissural pathways that provide access to the olfactory memory stored on the contralateral side. Furthermore, the commissural pathways need not be present at the time of memory formation but can establish new and specific access to already existing olfactory memories.     

Generation of a synthetic memory trace

We investigated the effect of activating a competing, artificially generated, neural representation on encoding of contextual fear memory in mice. We used a c-fos-based transgenic approach to introduce the hM(3)D(q) DREADD receptor (designer receptor exclusively activated by designer drug) into neurons naturally activated by sensory experience. Neural activity could then be specifically and inducibly increased in the hM(3)D(q)-expressing neurons by an exogenous ligand. When an ensemble of neurons for one context (ctxA) was artificially activated during conditioning in a distinct second context (ctxB), mice formed a hybrid memory representation. Reactivation of the artificially stimulated network within the conditioning context was required for retrieval of the memory, and the memory was specific for the spatial pattern of neurons artificially activated during learning. Similar stimulation impaired recall when not part of the initial conditioning.     

Requirement for hippocampal CA3 NMDA receptors in associative memory recall

Pattern completion, the ability to retrieve complete memories on the basis of incomplete sets of cues, is a crucial function of biological memory systems. The extensive recurrent connectivity of the CA3 area of hippocampus has led to suggestions that it might provide this function. We have tested this hypothesis by generating and analyzing a genetically engineered mouse strain in which the N-methyl-D-asparate (NMDA) receptor gene is ablated specifically in the CA3 pyramidal cells of adult mice. The mutant mice normally acquired and retrieved spatial reference memory in the Morris water maze, but they were impaired in retrieving this memory when presented with a fraction of the original cues. Similarly, hippocampal CA1 pyramidal cells in mutant mice displayed normal place-related activity in a full-cue environment but showed a reduction in activity upon partial cue removal. These results provide direct evidence for CA3 NMDA receptor involvement in associative memory recall.     

Effects of thiopental sedation on learning and memory

Subjects who were administered thiopental showed a loss of memory for events discussed while they were under sedation. We tested the subjects for recognition memory of pictures and recall of associated pairs of letters and words, and found that the subsequent memory loss was correlated with the concentration of thiopental in the venous blood at the time the material was learned. Retention did not appear to be state-dependent because the subject, while under sedation, could recall material learned prior to sedation, and because recall was not facilitated by reinstatement of the sedation.     

Internally generated cell assembly sequences in the rat hippocampus

A long-standing conjecture in neuroscience is that aspects of cognition depend on the brain's ability to self-generate sequential neuronal activity. We found that reliably and continually changing cell assemblies in the rat hippocampus appeared not only during spatial navigation but also in the absence of changing environmental or body-derived inputs. During the delay period of a memory task, each moment in time was characterized by the activity of a particular assembly of neurons. Identical initial conditions triggered a similar assembly sequence, whereas different conditions gave rise to different sequences, thereby predicting behavioral choices, including errors. Such sequences were not formed in control (nonmemory) tasks. We hypothesize that neuronal representations, evolved for encoding distance in spatial navigation, also support episodic recall and the planning of action sequences.     

Requirement for CD4 T cell help in generating functional CD8 T cell memory

Although primary CD8 responses to acute infections are independent of CD4 help, it is unknown whether a similar situation applies to secondary responses. We show that depletion of CD4 cells during the recall response has minimal effect, whereas depletion during the priming phase leads to reduced responses by memory CD8 cells to reinfection. Memory CD8 cells generated in CD4+/+ mice responded normally when transferred into CD4-/- hosts, whereas memory CD8 cells generated in CD4-/- mice mounted defective recall responses in CD4+/+ adoptive hosts. These results demonstrate a previously undescribed role for CD4 help in the development of functional CD8 memory.     

Sites of neocortical reorganization critical for remote spatial memory

The hippocampus is crucial for spatial memory formation, yet it does not store long-lasting memories. By combining functional brain imaging and region-specific neuronal inactivation in mice, we identified prefrontal and anterior cingulate cortices as critical for storage and retrieval of remote spatial memories [correction]. Imaging of activity-dependent genes also revealed an involvement of parietal and retrosplenial cortices during consolidation of remote memory. Long-term memory storage within some of these neocortical regions was accompanied by structural changes including synaptogenesis and laminar reorganization, concomitant with a functional disengagement of the hippocampus and posterior cingulate cortex [correction]. Thus, consolidation of spatial memory requires a time-dependent hippocampal-cortical dialogue, ultimately enabling widespread cortical networks to mediate effortful recall and use of cortically stored remote memories independently.     

Forgetting: trace erosion or retrieval failure?

A series of lists of random words was presented. Following each list, the subject attempted to recall the words of the list prior to the list just presented. Recall probability for a given word depended on the length of the list in which it was embedded, not on the length of the list intervening between presentation and test. These results indicate that forgetting is a failure in the memory search during retrieval rather than a degradation of the memory trace occurring between presentation and test.     

记忆原理在词汇教学中的应用

英语词汇记忆机制包括对语言材料的识记、保持、认知和重现四个重要环节, 为保证每个环节的贯通,在词汇教学中, 教师首先要指导学生优化输入, 从不同语境中习得词汇并掌握一定的记忆策略。其次,引入语段教学的概念, 扩大记忆的广度。最后, 根据记忆编码中的贴近原则, 要引导学生找出词汇内部的联系, 提高记忆的效果。     

Place cells and place recognition maintained by direct entorhinal-hippocampal circuitry

Place cells in hippocampal area CA1 may receive positional information from the intrahippocampal associative network in area CA3 or directly from the entorhinal cortex. To determine whether direct entorhinal connections support spatial firing and spatial memory, we removed all input from areas CA3 to CA1, thus isolating the CA1 area. Pyramidal cells in the isolated CA1 area developed sharp and stable place fields. Rats with an isolated CA1 area showed normal acquisition of an associative hippocampal-dependent spatial recognition task. Spatial recall was impaired. These results suggest that the hippocampus contains two functionally separable memory circuits: The direct entorhinal-CA1 system is sufficient for recollection-based recognition memory, but recall depends on intact CA3-CA1 connectivity.     

Brown rice planthopper (Nilaparvata lugens Stal) detection based on deep learning

The brown rice planthopper (Nilaparvata lugens Stal) is one of the main pests of rice. The rapid and accurate detection of brown rice planthoppers (BRPH) can help treat rice in time. Due to the small size, large number and complex background of BRPHs, image detection of them is challenging. In this paper, a two-layer detection algorithm based on deep learning technology is proposed to detect them. The algorithm for both layers is the Faster RCNN (regions with CNN features). To effectively utilize the computing resources, different feature extraction networks have been selected for each layer. In addition, the second layer detection network was optimized to improve the final detection performance. The detection results of the two-layer detection algorithm were compared with the detection results of the single-layer detection algorithm. The detection results of the two-layer detection algorithm for detecting different populations and numbers of BRPHs were tested, and the test results were compared with YOLO v3, a deep learning target detection network. The test results show that the detection results of the two-layer detection algorithm were significantly better than those of the single-layer detection algorithm. In the tests for different numbers of BRPHs, the average recall rate of this algorithm was 81.92%, and the average accuracy was 94.64%; meanwhile, the average recall rate of YOLO v3 was 57.12%, and the average accuracy rate was 97.36%. In the experiment with different ages of BRPHs, the average recall rate of the algorithm was 87.67%, and the average accuracy rate was 92.92%. In comparison, for the YOLO v3, the average recall rate was 49.60%, and the average accuracy rate was 96.48%.

     

Awake hippocampal sharp-wave ripples support spatial memory

The hippocampus is critical for spatial learning and memory. Hippocampal neurons in awake animals exhibit place field activity that encodes current location, as well as sharp-wave ripple (SWR) activity during which representations based on past experiences are often replayed. The relationship between these patterns of activity and the memory functions of the hippocampus is poorly understood. We interrupted awake SWRs in animals learning a spatial alternation task. We observed a specific learning and performance deficit that persisted throughout training. This deficit was associated with awake SWR activity, as SWR interruption left place field activity and post-experience SWR reactivation intact. These results provide a link between awake SWRs and hippocampal memory processes, which suggests that awake replay of memory-related information during SWRs supports learning and memory-guided decision-making.     

Marihuana and memory: acquisition or retrieval?

Two experiments were conducted to determine the means by which marihuana affects human memory. The results of these studies indicated that marihuana did not affect retrieval of information in memory when the method of free recall was used, but did affect recognition processes such that subjects were less able to discriminate between items that had been presented previously and items that had not appeared a short time before. With respect to initial learning, marihuana was shown to affect acquisition processes involved in the storage of information.     

一种大规模体数据压缩体绘制策略

针对基于GPU的大规模体数据直接体绘制过程中遇到的显存不足的问题，提出了一种大规模体数据的压缩绘制策略.该策略结合小波变换和分类矢量量化进行数据压缩，采用基于GPU的光线投射算法进行绘制，在绘制时，只解压变换当前绘制所需要的极少数数据，并结合多分辨率绘制，实现实时交互.基于CUDA的实验表明：该压缩绘制策略有效解决了显存不足问题，并且在保证交互绘制帧率的前提下获得了较好的成像质量.     

基于轻量化YOLOv4的生猪目标检测算法

针对目前生猪目标检测算法模型较大,实时性差导致其难以在移动终端中应用等问题,将一种改进的轻量化YOLOv4算法用于生猪目标检测.在群养猪环境下以不同视角和不同遮挡程度拍摄生猪图像,建立生猪目标检测数据集.基于轻量化思想,在YOLOv4基础上缩减模型大小.结果表明,本研究算法的准确率和召回率分别为96.85％和91.75...     

Maintenance of serological memory by polyclonal activation of human memory B cells

Production of antibodies can last for a lifetime, through mechanisms that remain poorly understood. Here, we show that human memory B lymphocytes proliferate and differentiate into plasma cells in response to polyclonal stimuli, such as bystander T cell help and CpG DNA. Furthermore, plasma cells secreting antibodies to recall antigens are produced in vivo at levels proportional to the frequency of specific memory B cells, even several years after antigenic stimulation. Although antigen boosting leads to a transient increase in specific antibody levels, ongoing polyclonal activation of memory B cells offers a means to maintain serological memory for a human lifetime.     

美国食品上市公司召回启动时间影响因素的实证分析

为探究食品召回启动时间的多重影响因素问题,以1994—2017年美国食品上市公司发生的召回事件为研究样本,构建多元线性回归模型,实证分析食品召回启动时间的影响因素。结果表明:召回启动时间的主要影响因素有召回量、分销范围和召回原因(细菌感染和外来物质污染);召回量对召回启动时间具有显著的正向影响;分销范围越小,召回启动时间越短;召回原因为细菌感染或外来物质污染时,召回启动时间相对更短;召回主体对召回启动时间没有表现出显著影响。本研究为完善我国食品召回体系,从公司、供应链和政府层面提出强化食品HACCP认证、完善食品安全溯源监管系统、建立专项储备基金制度等对策建议。     

Forms of memory failure

Memory may fail in a variety of ways. Patients with Korsakoff's syndrome demonstrate global memory deficits similar to those seen in patients with early progressive dementia. Korsakoff's patients, however, may recall rules and principles for organizing information and can gain access to their previously acquired knowledge (semantic memory), whereas recent memory may be grossly impaired. In contrast, dementia patients may have little access to previously acquired knowledge and therefore have great difficulty in organizing and encoding ongoing events. These contrasting forms of memory failure have implications for understanding the structure and mechanisms of memory and learning, particularly the relationship between episodic and semantic memory, as well as the development of therapeutic strategies for cognitive impairments.