Effects of varying energy intake and sire breed on duration of postpartum anestrus, insulin like growth factor-1, and growth hormone in mature crossbred cows

Objectives of this study were to evaluate effects of seven sire breed groups and three levels of daily ME intake (DMEI = 132 or 189 kcal ME/kg BW(0.75) or ad libitum), beginning 5 mo prepartum, on BCS, length of postpartum anestrus, and circulating concentrations of IGF-1 and GH in F1 cows (six to eight cows per sire breed in each DMEI group) out of Angus or Hereford dams. At the initiation of the study, BW were 522, 530, 548, 572, 575, 577, and 595 kg for cows sired by Longhorn, Galloway, 1960s Hereford or Angus, 1980s Hereford or Angus, or Nellore, Salers, and Shorthorn bulls, respectively (SE = 13; P < 0.001 for sire breed). After 4 mo on DMEI treatment during the pre-partum period, cows fed 132 kcal of ME/kg BW(0.75)gained little to no BW; cows fed 189 kcal ME/kg BW(0.75) gained 50 kg; and cows fed ad libitum gained 70 kg (all groups differ P < 0.05). Concentrations of progesterone in weekly blood samples collected 2 to 14 wk after calving were used to establish when normal luteal function resumed to predict length of postpartum anestrus. Length of anestrus was affected by level of DMEI in cows sired by Galloway, Longhorn, and Nellore bulls, but not other breeds (P < 0.02 for interaction of sire breed and DMEI). Level of DMEI, but not sire breed, affected (P < 0.01) BCS at wk 2 postpartum. Concentrations of IGF-1 at wk 2 postpartum differed (P < 0.001) due to sire breed, and changes in concentrations of IGF-1 from wk 2 to 14 were influenced (P < 0.03) by the interaction of sire breed and level of DMEI; which was primarily the result of differences in rate of decrease over time among different sire breed x level of DMEI groupings. Concentrations of GH did not differ due to sire breed but varied (P < 0.001) due to the interaction of DMEI and week postpartum, for which concentrations of GH did not differ at wk 2 but increased over time at rates that were inversely proportional to level of DMEI. Length of anestrus was negatively associated (P < 0.05) with day of calving, BCS, and BW. When effects of sire breed and level of DMEI were accounted for (residual correlation), length of anestrus was inversely associated (P < 0.01) with IGF-1 concentrations. Breed of sire influenced length of postpartum anestrus and energy balance, as predicted by IGF-1, in crossbred cows fed varying levels of DMEI.     

Effects of prepartum energy intake on steroids during late gestation and on cow and calf performance

At 50 d prior to predicted calving, 37 multiparous Angus cows were grouped by sire of mating, age and weight of cow and placed on either a high energy (HE, n = 19) diet or a moderate energy (ME, n = 18) diet. Objectives were to determine the effect of prepartum nutrition on: prepartum serum concentrations of estrone (E1), estrone sulfate (E1SO4) and progesterone (P4); pre- and postpartum cow body weight changes; calf birth weight and cow and calf postpartum performance. The ME cows were group-fed Coastal bermudagrass hay ad libitum and dormant pasture; HE cows were group-fed 2.7 kg ground corn X head-1 X d-1 in addition to the ME treatment. Both groups were combined and fed identically after calving. Cows fed HE were heavier (P less than .01) than cows fed ME at d 10 prepartum and their calves were heavier (P less than .05) at birth and weaning than calves from cows fed ME. Serum E1 concentrations were not significantly different between groups, but serum E1SO4 was higher (P less than .01) at d 10 prepartum in ME cows compared with HE cows. Serum P4 concentrations of ME cows were higher (P less than .05) than those of HE cows. Cow body weights were greater (P less than .01) for the HE group than for the ME group during the first 6 mo postpartum. Cow rebreeding performance was identical for both groups.     

Genetic x environment interactions on blood constituents of Angus, Brahman, and reciprocal-cross cows and calves grazing common bermudagrass or endophyte-infected tall fescue

Over a 2-yr period, effects of genotype and forage on blood metabolites, enzymes, and minerals were determined in Angus, Brahman, Angus x Brahman (sire x dam), and Brahman x Angus cows, and 129 calves from these cows sired by Hereford bulls. Cows and calves continuously grazed either common bermudagrass or endophyte-infected 'Kentucky-31' tall fescue pastures throughout the year. Blood samples were collected via jugular venipuncture in April, August, October (weaning), and November (after 30 d in a feedlot) of each year. Plasma urea N concentrations of cows and calves were affected by forage (P < 0.01) and breed (P < 0.05). Plasma cholesterol and FFA concentrations of cows were affected by forage (P < 0.01) and breed (P < 0.05). In calves, antibody titers to infectious bovine rhinotracheitis virus were not affected by forage but were affected by breed. Serum inorganic P concentrations of calves and cows were affected by forage (P < 0.05). Serum P concentrations and alkaline phosphatase activity of calves were affected by breed (P < 0.05). Calves grazing bermudagrass had higher (P < 0.05) serum concentrations of Fe and total iron binding capacity (TIBC). There was evidence of maternal heterosis for concentrations of free fatty acids, cholesterol, aspartate aminotransferase, Ca, Mg, alkaline phosphatase, ceruloplasmin, Fe, and TIBC. There was evidence of grandmaternal effects for plasma concentrations of urea N, cholesterol, Ca, P, Mg, and alkaline phosphatase. These results suggest that calves and cows grazing tall fescue are generally on a lower plane of nutrition than those grazing bermudagrass and that Brahman x Angus and Angus x Brahman crossbred cows and their calves seen to be more tolerant of the negative effects of tall fescue than the average of their purebred contemporaries.     

Comparison of F1 Bos indicus x Hereford cows in central Texas: I. Reproductive, maternal, and size traits.

Cows (n = 116) sired by Angus, Gray Brahman, Gir, Indu-Brazil, Nellore, and Red Brahman bulls and from Hereford dams were evaluated (when bred to the same breed of bull) for reproductive performance and cow weight and height and their calves (n = 1,161) were evaluated for birth and weaning weight in central Texas (temperate winters and subtropical summers). Cows were born from 1982 to 1985 and their calves were born from 1985 to 1999. Crossbred cows sired by Angus had a higher (P < 0.10) occurrence of calving difficulty than Gir, Indu-Brazil, Nellore, and Red Brahman crossbreds. Calves from Gir crossbreds had lighter (P < 0.10) birth weight (34.8 kg) than calves from Angus and Red Brahman crossbreds (39.4 and 37.2 kg). Calves from Angus crossbreds cows had lighter (P < 0.001) weaning weight (227.0 kg) than calves from Bos indicus crossbreds. Cows sired by Angus were lighter (P < 0.10) as mature cows (520.69 kg) than Gray Brahman, Indu-Brazil, and Red Brahman crossbreds (585.6, 571.9, and 577.6 kg, respectively). They also had smaller (P < 0.05) hip height (124.7 cm) than Bos indicus crossbreds. Mature cows sired by Indu-Brazil had greater (P < 0.05) hip height (138.3 cm) than Gir crossbreds (133.8 cm). Differences among sire breeds were noted within cow age categories (2 through 14 yr of age) for pregnancy rate, calf crop born, calf survival rate (as a trait of cow), and calf crop weaned. Angus and Indu-Brazil crossbred cows were lower (P < or = 0.10) than Gir and Nellore crossbreds for pregnancy rate, calf crop born, and(or) calf crop weaned at some, but not all, ages. Indu-Brazil crossbreds had a lower (P < or = 0.10) calf survival rate than most other breed groups at ages 11 to 14 yr. The results of this study indicate that Nellore and Gir crossbreds, but not Indu-Brazil, could perform as well or better than breeds traditionally used in cross-breeding programs of U.S. cow-calf production.     

Oxytocin-induced secretion of prostaglandin F2alpha in postpartum beef cows: effects of progesterone and estradiol-17beta treatment

The purpose of the present study was to determine the effect of progesterone or progesterone + estradiol-17beta on oxytocin-induced prostaglandin F2alpha (PGF2alpha) secretion in postpartum beef cows. Thirty-four anestrous postpartum beef cows were ovariectomized (d 32 [Groups 1 to 3] or d 23 [Groups 4 to 6] postpartum [d 0 = parturition]) and allotted to six treatments (Group 1; negative control) to simulate short (Groups 2 through 5) or normal (Group 6) length estrous cycles. Steroid treatments for the respective groups were as follows: Group 1) no estradiol-17beta or progesterone treatment (n = 8; negative control); Group 2) progesterone (d 34 to 40; n = 6); Group 3) estradiol-17beta (d 32 to 33) and progesterone (d 34 to 40; n = 6); Group 4) progesterone (d 23 to 29), no estradiol-17beta (d 32 to 33), and progesterone (d 34 to 40; n = 5); Group 5) progesterone (d 23 to 29), estradiol-17beta (d 32 to 33), and progesterone (d 34 to 40; n = 5); and Group 6) progesterone (d 23 to 29), estradiol-17beta (d 32 to 33), and progesterone (d 34 to 50; n = 4; positive control). Oxytocin (100 IU) was injected (i.v.) at the end of each treatment to test the ability of the postpartum uterus to secrete PGF2alpha as measured by a stable metabolite of PGF2alpha, 15keto-13,14 dihydro-PGF2alpha (PGFM). Peak concentrations ofPGFM (P < 0.08) and total PGFM secreted (area under the curve; P < 0.05) were increased on d 6 following first (Group 2) or second (Group 4) exposure to progesterone and were similar to peak concentrations and total PGFM secreted 16 d following a simulated normal estrous cycle (Group 6). Administration of estradiol-17beta before first progesterone exposure (Group 3) did not reduce peak concentrations of PGFM or total PGFM secreted relative to the preceding groups. Peak concentrations of PGFM (P < 0.08) and total PGFM secreted (P < 0.05) were reduced following a second progesterone exposure, provided that cows were pretreated with estradiol-17beta (Group 5). In summary, oxytocin-induced release of PGFM was inhibited on d 6 following second exposure to progesterone only when cows were pretreated with estradiol-17beta. Therefore, estradiol-17beta and progesterone were both associated with the timing of PGF2, secretion in postpartum cows.     

Effect of tropically adapted sire breeds on preweaning growth of F1 Angus calves and reproductive performance of their Angus dams

The objective of this study was to determine the effect of tropically adapted sire breeds on preweaning growth performance of F1 calves and on reproductive performance of their Angus dams. Angus (A) cows were bred in two consecutive years (1992 and 1993) by AI using semen from Brahman (B; Bos indicus; n = 10), Senepol (S; Bos taurus; n = 10), and Tuli (T; Sanga; n = 9) bulls. A total of 82 B x A, 85 S x A, and 91 T x A calves were born. The statistical model included the fixed effects of year, sire breed, calf sex, sire breed x calf sex, and cow parity and the random effect of sire within sire breed. Birth weight, weaning weight, 205-d adjusted weaning weight, ADG from birth to weaning, and hip height at weaning were greater (P < .001) for B x A calves than for S x A or T x A calves. Greater differences were detected between sexes for B x A than for S x A and T x A (for all traits sire breed x calf sex, P < .05). Sire breed affected (P < .01) the percentage of unassisted calvings (B x A, 87%; S x A, 98%; and T x A, 100%) and tended (P < .10) to affect the percentage of calves that survived until weaning (B x A, 90%; S x A, 94%; and T x A, 98%). Sire breed of calf did not affect (P > .10) length of gestation, and sire breed did not affect the interval from calving to first observed estrus or pregnancy in Angus dams. These results demonstrate that preweaning growth performance of B x A calves was greater than that of either S x A or T x A calves. However, use of Brahman sires on Angus dams led to calving problems and tended to reduce the percentage of calves that survived until weaning. Thus, heavier weaning weights of B x A calves would be an advantage for cow-calf producers marketing calves, but heavier birth weights and calving difficulty attributed to Brahman sires would be a disadvantage.     

Comparison of F1 Bos indicus x Hereford cows in central Texas: II. Udder, mouth, longevity, and lifetime productivity.

Postpartum udder characteristics, aged mouth condition scores, and cow survival, longevity, and productivity were evaluated for 15 yr on F1 cows in central Texas (temperate winters and subtropical summers). The cows (n = 116) were progeny of Angus, Gray Brahman, Gir, Indu-Brazil, Nellore, and Red Brahman sires and Hereford dams. Crossbred cows sired by Nellore had smaller (P < 0.05) postpartum teat length than cows of all other crossbred groups and smaller (P < 0.10) postpartum teat diameter than Indu-Brazil and Red Brahman crossbreds. The Nellore crossbred cows had larger (P < 0.10) postpartum udder support scores (more well-supported udders) than Gir, Indu-Brazil, and Red Brahman crossbreds. As 14-yr-old cows, Angus crossbreds had lower (P < 0.05) mouth scores (indicative of one or more missing incisors) than Bos indicus crossbreds. Under actual and artificial (records were removed) culling procedures, Nellore crossbreds had the highest cow survival to age 14 and the highest longevity. Indu-Brazil crossbreds tended to be the lowest for both survival and longevity. Curves were fitted by nonlinear regression procedures to the adjusted frequencies of cows remaining at each age; scale and shape parameters were estimated and compared for the different breed groups. Breed group cow survival rates, calf crop weaned, and calf weaning weights were used 1) to construct population projection and production matrices for each breed group and 2) to generate breed group vectors containing projected age distributions and production information. Cows sired by Nellore and Gir were projected to require the fewest purebred cows to generate replacement heifers and to have the most calves and the largest total calf weight sold per year.     

Changes in pelvic conformation and peripheral estrone concentrations in pre- and post-partum beef cows

An experiment was conducted to investigate the temporal relationship of peripheral estrone (E1) concentration to changes in the size of the pelvic opening preceding and immediately following parturition. Twenty-six multiparous beef cows were observed from approximately 50 d prepartum to 7 d postpartum. Blood samples were collected at 7 d intervals preceding calving and at 1, 3 and 7 d following for E1 quantitation. Estimates of pelvic opening area were made at the time of blood sampling. Peripheral E1 concentrations were elevated beginning at approximately 25 d prepartum. Dams bearing male fetuses had greater (P less than 0.01) concentrations of E1 than did dams with female fetuses. Calf birth weight was correlated (r = 0.44, P less than 0.01) with E1 levels from 10 d prepartum through parturition. Postpartum pelvic area was greater for cows giving birth to male calves, with no significant differences for calf birth weights by sex. Correlations were observed between E1 concentration, and pelvic area measured from 50 d prepartum to 7 d postpartum (r = 0.26, P less than 0.01), 10 d prepartum to calving (r = 0.42, P less than 0.01), and from calving to 7 d postpartum (r = 0.33, P less than 0.01). Percentage increase in E1 concentration from 50 d prepartum to calving was significantly correlated (r = 0.75, P less than 0.01) to percentage pelvic area increase over the same period. A correlation also exists between maternal E1 concentrations and fetal sex and pelvic area. In summary, the increased estrogen concentrations in cows with male calves may facilitate pelvic spread, resulting in a larger pelvic opening.     

Effects of heat stress during pregnancy on postpartum reproductive changes in Holstein cows

Effects of heat stress during the last third of gestation on reproductive changes postpartum were studied in Holstein cows. Cows and heifers 160 to 190 d of gestation were assigned in June 1978 to shade (n = seven cows and two heifers) or no shade (n = eight cows and two heifers) management treatments. After parturition, all cows (n = 19) were moved to the milking herd and managed uniformly. On the day of calving and on each Monday, Wednesday and Friday thereafter until d 50 postpartum, jugular blood samples were collected. Beginning approximately 7 d postpartum, the reproductive tract of each cow was examined rectally after collection of blood samples. Estrus was monitored twice daily and cows were inseminated after d 45 postpartum. Prepartum heat stress increased 13,14-dihydro-15-keto-prostaglandin F2 alpha (PGFM) concentrations postpartum and increased the rate of uterine involution. Regardless of prepartum treatment, progesterone concentrations indicated that luteal phases had begun by d 12.4 +/- 1.3 postpartum, which was about 3 d before PGFM was basal. The first luteal phase lasted only 10.7 +/- .9 d. First estrus was not detected until d 32.3 +/- 4.8 postpartum. The previously gravid uterine horn had a negative effect on ovarian volume, diameter of the largest follicle and percentage of ovaries with a corpus luteum. However, prepartum heat stress attenuated this effect. This study indicates that heat stress prepartum had residual effects on postpartum reproductive changes, and that the previously gravid uterine horn exerted some control, which was attenuated by heat stress, over ovarian recrudescense. Even though heat stress prepartum affected sensitive measures of postpartum reproductive function, it did not alter days to first estrus, days open (102.3 +/- 13.1) or services/conception (2.5 +/- .3).     

Techniques for estimation of milk yield in beef cows and relationships of milk yield to calf weight gain and postpartum reproduction

Milk production is a primary factor controlling weaning weight of beef calves. The purpose of this experiment was to develop a practical and reliable on-farm method of estimating milk production and to relate estimates of milk production and milk composition to preweaning weight gain of calves. A second objective was to relate milk production to postpartum ovarian activity. Milk production of spring- and fall-calving grade Angus cows was estimated by machine milking (MM; average of 66, 123 and 189 d postpartum) and weigh-suckle-weigh (WSW; average of 50, 95, 136 and 179 d postpartum) techniques following overnight calf removal. Cows and calves were weighed monthly and the postpartum interval to first ovulation following calving was determined. The repeatability of the estimated milk production by MM (.97) was higher (P less than .01) than by WSW (.35). The correlation of average WSW and average MM estimates of milk production with preweaning calf gain were high and similar (greater than .75). Inclusion of milk composition did not improve the multiple correlation of MM-estimated milk production and calf gain. Neither milk production in early lactation (-.06) nor prebreeding weight change (-.07) was correlated with postpartum interval to ovulation. Machine milking was a repeatable method of estimating milk production of beef cows and can be used to evaluate effects of management variables on lactation of beef cows.     

Influence of dietary energy on follicular development, serum gonadotropins, and first postpartum ovulation in suckled beef cows

Twenty-eight Hereford x Angus cows (4 yr of age) were used to determine the effects of pre- and postpartum dietary energy on performance and reproductive function in suckled beef cows. The experiment was designed as a 2 x 2 factorial with cows receiving either 70 (L) or 150% (H) of NRC recommended level of dietary energy before and(or) after parturition, resulting in four treatment combinations (L-L, L-H, H-L, H-H). Prepartum diets were fed for approximately 110 d, and postpartum diets were fed until either 10 d after the second postpartum ovulation or 150 d postpartum for those cows that failed to ovulate. Cows receiving low compared with high levels of energy before calving lost more (P less than .01) weight, body condition, subcutaneous fat, and longissimus muscle area before parturition and had calves with lighter (P less than .01) birth weights. Cows receiving low compared with high levels of energy postpartum lost more (P less than .01) weight, body condition, and longissimus muscle area after parturition. Low levels of energy before and after parturition decreased (P less than .01) milk production and calf weight at 70 d of age. Rates of cervical and uterine involution were unaffected by dietary energy treatments. Cows receiving high levels of energy prepartum had increased (P less than .01) mean concentrations and pulse frequency of LH in serum after parturition, and cows receiving high levels of energy postpartum had increased (P less than .05) pulse frequency of LH. Low levels of energy postpartum decreased (P less than .01) appearance rate of small (5.0 to 7.9 mm) and large (greater than or equal to 10 mm) follicles, and low levels of energy prepartum decreased (P less than .02) appearance rate of large follicles based on transrectal ultrasonography. Cows receiving high levels of energy prepartum had shorter (P less than .02) intervals from parturition to ovulation, and a higher (P less than .01) percentage of the cows that received high levels of energy postpartum ovulated by 150 d postpartum. In summary, prepartum level of dietary energy influenced birth weight and weight gain of calves, milk production, concentrations and pulse frequency of LH in serum, appearance rate of large follicles, and the interval to first ovulation. Postpartum level of dietary energy influenced milk production, weight gain of calves, pulse frequency of LH, appearance rate of small and large follicles, and the percentage of cows that ovulated after parturition.     

Differences among breed crosses of cattle in the conversion of food energy to calf weight during the preweaning interval

The objective of this study was to determine whether F1 cows that differ in genetic potential for weight at maturity and milk yield vary in the conversion of food energy to calf weight gain. Food intakes and weight change data were recorded by pen for cows and calves from approximately 45 d postpartum. Cows assigned to the study were 7- to 9-yr-old F1s produced by top-crossing Angus, Hereford, Brown Swiss, Chianina, Gelbvieh; Maine Anjou, and Red Poll sires to either Angus or Hereford dams. Calves were sired by Simmentals. Experimental units were pens (10 to 12 cow/calf pairs); pen was replicated within breed of sire in each of 2 yr (n = 24). Calf weight gain and energy consumed by the dams differed among the F1s, as did the ratio of calf weight gain to energy consumed by the calf and cow. Angus or Hereford (35.8), Red Poll (35.7), or Maine Anjou (35.6) F1s produced more calf weight per unit of energy consumed (g/Mcal) by the cow and calf than Chianina (33.1) or Gelbvieh (33.7) F1 females; Brown Swiss cows were intermediate (34.3). Differences in food conversion efficiency exist among breed crosses. These differences seem to be associated with breed cross differences in genetic potential for milk yield and mature weight; an exception to this trend was the Maine Anjou.     

Cow breeding and calf growth performance as affected by pregnancy status the previous year

A 2-yr study was conducted to compare the subsequent cow breeding and calf performance of cows that were nonpregnant with cows that were pregnant at the time calves were weaned. Cows were Angus (A), Polled Hereford (PH), Santa Gertrudis (SG) straightbreds and crossbreds of these breeds. Nonpregnant cows (G1) were 4- to 9-yr-olds that had a calf the previous year and appeared to be physically sound with no detection (by rectal palpation) of an abnormal reproductive tract due to disease, abnormal growth or calving difficulties. Pregnant cows (G2) were of similar age and breed composition to G1 cows. The 93 G1 and the 193 G2 cows were assigned within age and breed composition to sire breeding groups on pasture in an approximate 1:2 ratio, respectively, per sire. There were six A, three PH and one SG sires. The year prior to G1 cows being nonpregnant, G1 cows calved 11 d later (P less than .01) than G2 cows. Subsequent to their being nonpregnant, G1 cows gained 27 kg more (P less than .001) weight during the breeding period, had 5.4 percentage units more (P less than .29) calves born, had calves 17 d earlier (P less than .001) in the calving period, had calves that gained at a similar rate to weaning and had calves that were 14 kg heavier (P less than .01) at weaning (due to their being 17 d older) compared with G2 cows and calves.     

Influence of Urea Alone or Combined with Fish Meal and Solubles in Liquid Supplements on Performance of Grazing Cow-Calf Pairs

Experiments were conducted for 84 d, beginning on July 1, with cow-calf pairs grazing summer grass to determine the effects of feeding molasses containing urea or urea, fish solubles, and fish meal on BW gains, milk production, and blood metabolites. The four treatments based on a 16% CP urea-molasses mixture were no supplement, 97.8% base mixture plus 2.2% urea for cows and calves, 80% base mixture plus 10% fish solubles and 10% fish meal for cows and calves, and the supplement with fish solubles and fish meal as a creep feed for calves. The mixture contained 23% CP as fed. In Exp. 1, 60 crossbred cows with Angus-sired calves were allotted according to sex and birth date to 12, 2-ha pastures. Three pastures each with five cow-calf pairs were assigned to each treatment. In Exp. 2, 48 Hereford X Brahman F₁ cows with Angus- or Belgian Blue-sired calves were allotted as in Exp. 1 to 24, 1-ha Alicia bermudagrass pastures. Three pastures with two cow-calf pairs were assigned to each treatment for each sire breed of calf. Cows were machine-milked, and jugular blood samples were collected initially and on d 28 and 56. Cows and calves were weighed, and cows were condition-scored after a 16-h shrink at the beginning and end of both experiments. Both supplements were consumed at similar levels (1.75 kg/d) by cow-calf pairs in both experiments. Cows in Exp. 1 and calves in both experiments gained more (P<0.05) BW when cow-calf pairs had access to the supplement containing fish solubles and fish meal. Milk yield was numerically higher on d 28 (P<0.18) and d 56 (P<0.11) when cows received fish solubles and fish meal in the supplement. The decline in milk yield from initial milking tended to be less (P<0.10) on d 28 and was less (P<0.05) on d 56 when cows received this supplement. Plasma ammonia N concentrations were higher (P<0.05) when cows were fed supplement containing only urea, and both supplements increased (P<0.05) plasma urea N when compared with no supplement. Supplements containing molasses, urea, fish solubles, and fish meal appear beneficial for cow-calf pairs grazing summer forage.     

Immune responses during the peripartum period in dairy cows with postpartum endometritis

Determining the immune responses to the development of endometritis during the peripartum period may assist in the development of more efficient reproductive management regimens for dairy herds. In this study, we compared the peripartum immune responses of dairy cows that develop endometritis by 4 weeks postpartum (n = 11) to cows that did not develop this disease (n = 19). Blood samples were collected 1 week before calving, just after or during calving, and then at weeks 1, 2, 3, and 4 postpartum. Cows that developed endometriris had significantly higher total leukocyte, neutrophil, lymphocyte, and monocyte counts than the control cows (P < 0.05) at all time points. The leukocytes from cows that developed endometritis were significantly less phagocytic than those from control cows at all sampling time points (P < 0.01). The serum TNFalpha concentrations of the control cows decreased linearly from the prepartum time point (P = 0.0029), but the endometritis cows showed a different profile (P > 0.05). As a result, the serum TNFalpha concentrations were greater in the endometritis group (P < 0.01) than in the control group during the third and fourth weeks postpartum. The greater total leukocyte numbers and neutrophil, lymphocyte and monocyte counts, and the maintenance of elevated serum TNFalpha levels in the cows with endometritis may be due to infection in the postpartum period. Furthermore, the decreased phagocytic capacity of leukocytes during the peripartum period, including at the prepartum time point, makes cows more susceptible to postpartum endometritis.     

Output/input differences among nonpregnant, lactating Bos indicus-Bos taurus and Bos taurus-Bos taurus F1 cross cows

Nonpregnant F1 crossbred cows, progeny of either Hereford (H) or Angus (A) dams and sired by Brahman (Bm), Sahiwal (Sw), Pinzgauer (Pz), H, or A sires, were fed to maintain initial weight while rearing Charolais (C)-sired progeny for a period of 126 d in drylot commencing at about 48 d postpartum. Cow-calf pairs were assigned to equalize cow age, calf sex, and breed of cow's dam among three replicate pens of approximately 12 pairs each. Cows and calves were weighed every 2 wk and feed intake was adjusted to minimize change in cow weight. Metabolizable energy (ME) consumption for zero cow weight change was estimated by regression. Milk production was estimated by weight-suckle-weigh at 58, 85, 125, and 170 d of lactation. Calf gain (GAIN, kg) relative to cow weight (CWT1, kg) was higher (P less than .01) for calves from Bm-X (139.5/585) and Sw-X (132.2/534) than for calves from Pz-X (127.2/552) and HA-X (116.9/547) cows. Estimated mean daily production of milk was 7.40, 7.15, 7.28, and 6.37 kg for the Bm-X, Sw-X, Pz-X, and HA-X, respectively. Total cow ME intake (TMEcow) for breed groups ranked (P less than .05) with cow size and milk production, and calf creep-feed intake (FMEcalf) was inversely related to estimated milk intake. Proportion of total feed ME (TMEcow+calf) consumed by calves was higher (P less than .05) for HA-X cows (18%) than for the others (14%). Total efficiency of calf gain in weight (GAIN/TMEcow+calf) was 11% greater (P less than .05) for crossbred cows of Bos indicus X Bos taurus (Bm-X, Sw-X) than for Bos taurus X Bos taurus (Pz-X, HA-X) cows (35 vs 32 g/Mcal) in the 126-d lactation period.     

Effects of oral vitamin E supplementation during late gestation in beef cattle that calved in late winter and late summer

OBJECTIVE: To determine effects of breed and oral vitamin E supplementation during late gestation on serum vitamin E and IgG concentrations in beef cows that calved in late winter and late summer and in neonatal calves. ANIMALS: 73 Angus and 43 Hereford primiparous and multiparous cows and their calves. PROCEDURE: Cows in groups that were homogeneous regarding breed and age distribution were randomly allotted to groups that were orally supplemented (n = 59) or not supplemented (57) with vitamin E beginning 30 days prior to onset of 65-day calving seasons. Supplemental vitamin E was provided in a vitamin-mineral mix offered free-choice until parturition. RESULTS: Cows that calved in late winter and were supplemented orally with vitamin E had higher serum vitamin E concentrations at calving and after calving than did unsupplemented cows; differences between groups before calving were not significant. Calves from supplemented multiparous cows had higher vitamin E concentrations than did calves from unsupplemented cows. Winter-born calves from supplemented Hereford cows had heavier 205-day adjusted weaning weights than did winter-born calves from unsupplemented Hereford cows. Supplementation did not affect vitamin E or IgG concentrations in the herd that calved in late summer and did not affect calf growth. CONCLUSIONS AND CLINICAL RELEVANCE: Oral vitamin E supplementation during late gestation may be economically beneficial in certain cow-calf operations in which late-gestation cows are consuming stored forages.     

Genetic effects on acute phase protein response to the stresses of weaning and transportation in beef calves

The objective herein was to estimate heterosis and breed effects in purebred and crossbred Romosinuano, Brahman, and Angus calves on acute phase protein response to weaning and transportation. Calves (n = 1,032) were weaned in September of 2002, 2003, and 2004 at approximately 7 mo of age. Approximately 28 d after weaning, steer calves (n = 482) were transported 1,800 km (20 h) to Oklahoma. Concentrations of 3 acute phase proteins (ceruloplasmin, fibrinogen, and haptoglobin) were measured in blood samples. Calves (steers and heifers) were sampled at weaning, and 24 and 72 h postweaning. For separate analyses, steers sent to Oklahoma were sampled before shipment, upon arrival, and 24 and 72 h after arrival. Combinations of the following fixed effects were investigated: sire breed, dam breed, sampling time, birth location, calf sex (weaning only), year, cow age, and interactions. Effects of special interest were sire breed x dam breed as an indication of breed group of calf, and the interaction of sire and dam breeds with sampling time. Weaning age and BW were investigated as linear and quadratic covariates. Sire of calf within sire breed was a random term. The correlation structure of repeated measures was determined by comparison of information criterion values for different structures within each analysis. In general, plasma acute phase protein concentrations in weaned calves increased with sampling time. Concentrations in the transported steers increased through sampling at 24 h after arrival, and were lower at 72 h. Significant estimates of heterosis were detected for Brahman-Angus haptoglobin concentrations at weaning (0.38 +/- 0.14 mg/dL x 100; 44%), and for Romosinuano-Angus fibrinogen concentrations at weaning (11.4 +/- 5.5 mg/dL; 10%) and in transported steers (22.5 +/- 8.4 mg/dL; 20%). The direct effect of Romosinuano was to increase (P <0.004) ceruloplasmin concentrations of weaned calves (4.1 +/- 0.9 mg/dL) and of transported steers (3.9 +/- 1.3 mg/dL). The direct effect of Angus was to lower ceruloplasmin concentrations in weaned calves (-3.9 +/- 1.2; P = 0.001). Significant maternal effects were detected at weaning for ceruloplasmin concentrations in Romosinuano (-1.4 +/- 0.5 mg/ dL) and Angus (1.6 +/- 0.7 mg/dL) and fibrinogen concentrations in Brahman calves (-17.7 +/- 8.8 mg/dL). These data imply that acute phase protein concentrations in response to weaning and transportation are impacted by cattle breed.     

Breed effects on crossbred cow-calf performance

Effects of seven breeds of cow's sire and 12 breeds of cow's maternal grandsire on preweaning performance of crossbred cows and their calves were examined in data from two experiments conducted at the Roman L. Hruska U.S. Mean Animal Research Center. Data included 1,836 records over three to five parities for 516 cows by 143 sires and by 307 maternal grandsires. The statistical model fitted effects of calf sex, parity, cow birth-breeding year or cow-calf birth year, the breed effects and their interactions. Deviations of breed of sire or equivalent grandsire effects on each trait from the mean for Hereford x Angus cows ranged from -1.6 to 5.5 kg (P less than .001) for calf birth weights, -15 to 1% (P less than .001) for calving difficulty, nonsignificant for preweaning calf mortality and -2 to 27 kg (P less than .001) for calf weaning weight. Deviations were nonsignificant for conception rate and calves weaned per cow exposed to breeding, but -2 to 40 kg (P less than .001) for calf weight weaned per cow exposed for breeding, -7 to 78 kg (P less than .001) for cow weight and -20 to 2% (P less than .001) for body condition score. The advantages of Holstein and Brahman cross over Hereford x Angus cows of 23 and 13% in weight of calf weaned/cow-breeding exposure must be compared with the expected greater feed requirements from 7 or 8% heavier cows and at least 50% higher milk production, which emphasizes the need to include input measures and costs in breed evaluation schemes.     

Influence of Prepartum Fat Supplementation on Subsequent Beef Cow Reproduction and Calf Performance

High fat range supplement (HFRS) and HFRS with lipid from soybean soapstock (HFRS-SPH; Consolidated Nutrition, Omaha, NE) were compared with a corn-soybean meal supplement (control). In Exp. 1, primiparous cows were individually fed the control supplement (n = 12), HFRS (n = 12), or HFRS-SPH (n = 10) for 62 ± 2 d prepartum. Heifer body condition score pre- and postpartum did not differ (P=0.78) among groups. Milk production was not influenced (P=0.15) by source of supplement. Somatic cell counts, however, tended to be less (P=0.07) in HFRS-supplemented heifers than in heifers fed the control supplement. At birth, calf body temperature (P=0.8), vigor (P=0.7), and BW (P=0.6), as well as BW gain through 90 d postpartum (P=0.6), did not differ among prepartum supplementation treatments. Plasma concentrations of linoleic acid were greater (P=0.02) in fat-supplemented heifers at 30 d prepartum and at calving compared with heifers on the control treatment; however, concentrations of plasma linoleic acid returned to levels comparable with those in control heifers by 30 d postpartum. Neither number of cows cycling by 90 d postpartum (P=0.15) nor length of the postpartum interval (P=0.25) differed among treatment groups. In Exp. 2, multiparous cows were pen-fed the control supplement (n = 49), HFRS (n = 47), or HFRS-SPH (n = 49) for 59 ± 2 d prepartum. Prior to parturition, cows fed the control supplement had better body condition scores (5.8 ± 0.1; P=0.004) than cows fed either commercial supplement (5.4 ± 0.1). Calf performance (P=0.7) and conception rates (P= 0.5) did not differ among treatments. Productivity of cows and calves was not improved with provision of supplemental fat prepartum.