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1.
Ahmed A Anjum FM Ur Rehman S Randhawa MA Farooq U 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):7-13
The bioavailability and interactive effects of calcium, iron and zinc fortified whole wheat flour chapattis on female Sprague–Dawley
albino rats were determined. The whole wheat flour was fortified with CaCO3, FeSO4 and ZnSO4 at 1000, 40 and 20 mg/kg, respectively and fed to 64 female Sprague–Dawley rats for 4 weeks. Retention of Ca, Fe and Zn was
determined in plasma, femur and liver tissues. Ca levels in plasma and liver did not alter significantly while the Ca retention
in femur changed significantly with the change in fortificants. The calcium retention was higher in the femur of rats fed
on the diet supplemented with Ca + Zn (221 ± 13.61 mg/g) than the rats fed on the diet supplemented with Ca + Fe (219 ± 18.92 mg/g).
The iron in plasma, liver and femur ranged from 2.19 ± 0.11 to 2.77 ± 0.10 μg/ml, 203.5 ± 13.24 to 251.5 ± 17.19 μg/g and
43.5 ± 1.05 to 52 ± 2.07 μg/g while zinc content ranged from 1.31 ± 0.089 to 1.55 ± 0.114 μg/ml, 89.5 ± 3.41 to 132 ± 6.10 μg/g
and 175 ± 8.29 to 191 ± 10.85 μg/g, respectively. The minerals interacted with each other resulting in decrease of bioavailability
among each other. The absorption was higher in the rats fed on fortified diet than those fed on unfortified chapatti diet.
Hence, it is concluded that fortification of whole wheat flour is feasible to overcome the Ca, Fe and Zn malnutrition in the
vulnerable groups. 相似文献
2.
Dejia Zhang Deloy G. Hendricks Arthur W. Mahoney 《Plant foods for human nutrition (Dordrecht, Netherlands)》1992,42(2):97-108
Effects of coprophagy and coprophagy preventing device on iron bioavailability were evaluated in two experiments. In Experiment 1, rats were fed diets with FeSO4, spinach, bran cereal or cornmeal as the iron source. The rats in each diet group were fitted with collars, sham-collars or not fitted with collars (control). In Experiment 2, rats were fed diets with FeSo4 or green peas as the iron source and were fitted with collars, tail cups, sham-collars or not fitted with any device (control). Preventing coprophagy reduced hemoglobin regeneration efficiency (HRE) of rats fed bran cereal, green peas, spinach or cornmeal diets by 26 (P<.05), 24(P<.05), 22(P<.05) and 11% (not significant), respectively. Preventing coprophagy in rats fed FeSO4 diet did not significantly reduce HRE. Sham-collaring reduced (P<.05) HRE of rats fed the FeSO4 diet by 12 and 13% but did not significantly affect HRE in rats fed food iron sources. It was more convenient and effective to prevent coprophagy with collars than with tail cups. Differences in bioavailability between food and FeSO4 iron due to coprophagy may be explained based on two gastrointestinal nonheme iron pools, complexed and highly soluble. 相似文献
3.
Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Bunkerd Kongyingyoes Upa kukongviriyapan 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):116-121
In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration
of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced
diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the
effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33
and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group
(6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC50 of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These
findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic
diabetic rats, which may be beneficial as food supplement for diabetics. 相似文献
4.
Korekar G Stobdan T Arora R Yadav A Singh SB 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):376-383
Fourteen apricot genotypes grown under similar cultural practices in Trans-Himalayan Ladakh region were studied to find out
the influence of genotype on antioxidant capacity and total phenolic content (TPC) of apricot kernel. The kernels were found
to be rich in TPC ranging from 92.2 to 162.1 mg gallic acid equivalent/100 g. The free radical-scavenging activity in terms
of inhibitory concentration (IC50) ranged from 43.8 to 123.4 mg/ml and ferric reducing antioxidant potential (FRAP) from 154.1 to 243.6 FeSO4.7H2O μg/ml. A variation of 1–1.7 fold in total phenolic content, 1–2.8 fold in IC50 by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and 1–1.6 fold in ferric reducing antioxidant potential among the examined
kernels underlines the important role played by genetic background for determining the phenolic content and antioxidant potential
of apricot kernel. A positive significant correlation between TPC and FRAP (r = 0.671) was found. No significant correlation was found between TPC and IC50; FRAP and IC50; TPC and physical properties of kernel. Principal component analysis demonstrated that genotypic effect is more pronounced
towards TPC and total antioxidant capacity (TAC) content in apricot kernel while the contribution of seed and kernel physical
properties are not highly significant. 相似文献
5.
Jimenez M Guzman AP Azuara E Garcia O Mendoza MR Beristain CI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):57-63
Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional
medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude,
aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high
antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing
power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of
vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential
(561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal,
trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants. 相似文献
6.
Alison Gegios Rachel Amthor Busie Maziya-Dixon Chedozie Egesi Sally Mallowa Rhoda Nungo Simon Gichuki Ada Mbanaso Mark J. Manary 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):64-70
Cassava contains little zinc, iron, and β-carotene, yet it is the primary staple crop of over 250 million Africans. This study
used a 24-hour dietary recall to test the hypothesis that among healthy children aged 2–5 years in Nigeria and Kenya, cassava’s
contribution to the childrens’ daily diets is inversely related to intakes of zinc, iron, and vitamin A. Dietary and demographic
data and anthropometric measurements were collected from 449 Kenyan and 793 Nigerian children. Among Kenyan children 89% derived
at least 25% of their dietary energy from cassava, while among the Nigerian children 31% derived at least 25% of energy from
cassava. Spearman’s correlation coefficient between the fraction of dietary energy obtained from cassava and vitamin A intake
was r = −0.15, P < 0.0001, zinc intake was r = −0.11, P < 0.0001 and iron intake was r = −0.36, P < 0.0001. In Kenya, 59% of children consumed adequate vitamin A, 22% iron, and 31% zinc. In Nigeria, 17% of children had
adequate intake of vitamin A, 57% iron, and 41% zinc. Consumption of cassava is a risk factor for inadequate vitamin A, zinc
and/or iron intake. 相似文献
7.
Mario Sanz-Penella J Laparra JM Sanz Y Haros M 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):50-56
The objective of the present study was to evaluate the effect of the bread supplemented with whole amaranth flour (0, 20 and
40%) on iron bioavailability using Caco-2 cells model. The phytate and lower myo-inositol phosphates content in in vitro bread digests were measured by high pressure liquid chromatography. The breads made with amaranth showed significant increase
of soluble phytates levels (up to 1.20 μmol/g in dry matter for the 40% of substitution) in comparison with controls, which have not detectable values. A negative
correlation among phytate and Fe availability was found when increased levels of amaranth. Ferritin concentration was found
2.7- and 2.0-fold higher (P < 0.05) in cultures exposed to 20% and 40% of amaranth formulated bread samples, respectively, compared to control bread.
The soluble phytate/Fe molar ratio explained the whole amaranth flour-mediated inhibitory effect associated to the limitation
of available Fe; however, the use up to 20% of amaranth in bread formulation appears as a promising strategy to improve the
nutritional value of bread, as indicated by the ferritin concentrations quantified in cell cultures. Higher proportion of
amaranth flour increased Fe concentration although there was not detected any increase in Fe uptake. 相似文献
8.
Ajibola CF Eleyinmi AF Aluko RE 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):320-327
The aim of this study was to determine the in vitro modulation of the renin-angiotensin system by polyphenolic extracts and fractions of two green leafy vegetables, Vernonia amygdalina (VA) and Gongronema latifolium (GL), that are used for food and medicinal purposes. An 80% acetone extract of each leaf was fractionated on silicic acid-packed
column to give two main fractions: acetone eluate (flow-through) and ethanol eluate (column-bound), that consist mostly of
chlorophyllic and non-chlorophyllic fractions, respectively. Column fractionation resulted in polyphenolic fractions that
displayed higher potency against angiotensin converting enzyme (ACE) and renin than the crude acetone extracts; generally,
the chlorophyllic fraction was more active than the non-chlorophyllic fraction. ACE-inhibitory activity was significantly
higher (p < 0.05) for the chlorophyllic fraction of VA than GL, with IC50 values of 0.207 and 0.413 mg/ml, respectively. Similarly, the chlorophyllic fraction of VA had significantly higher (p < 0.05) renin inhibition than GL, with IC50 values of 0.172 and 0.513 mg/ml, respectively. Kinetics studies showed that the chlorophyllic fractions of VA and GL exhibited
mostly mixed-type ACE and renin inhibitions. We concluded that the hydrophobic nature of the chlorophyllic fraction may have
contributed to the increased interaction with enzyme protein and inhibition of activities of ACE and renin. 相似文献
9.
A natural colorant was extracted fromCassia tora L. using buffer solutions (pH: 2–11) as extractants. The dyeing solution (Cassia tora L. extract) extracted using pH 9 buffer solution was found to give the highest K/S values of dyed fabrics. Cotton and silk
fabrics were dyed withCassia tora L. extract at 60°C for 60 min with pre-treatment of various metal salts as mordants. It was found thatCassia tora L. extract was polygenetic dyestuffs and its major components were anthraquinones. Studies have been made on the effects of
the kind of mordant on dyeing properties and colour fastnesses of cotton and silk fabrics. The K/S of cotton fabrics increased
in the order of the dyeing using FeSO4>CuSO4>ZnSO4>MnSO4≅Al2(SO4)3>NiSO4>none, however, the K/S of silk fabrics increased in the order of the dyeing using FeSO4>CuSO4>ZnSO4≅Al2(SO4)3>MnSO4≅NiSO4>none. It was found that the K/S values of dyed fabrics were largely affected by the colour difference (ΔE) between mordanted
fabric and control fabric. However, they were not depended on the content of mordanted metal ion of the fabrics. Mordants
FeSO4 and CuSO4 for cotton fabric, FeSO4, CuSO4, and Al2(SO4)3 for silk fabric were found to give good light fastness (rating 4). 相似文献
10.
Julio Beltrame Daleprane Tatiana Silveira Feijó Gilson Teles Boaventura 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(1):1-5
The aim of this study was to evaluate the protein quality of organic and genetically modified soy by feeding specific diets
to rats. Three groups of Wistar rats (n = 10) were used, and each group was named according to the food that they ate. There was an organic soy group (OG), a genetically
modified soy group (GG), and a control group (CG). All animals received water and diet ad libitum for 455 days. At the end of this period, the weight of the GG group was the same as that of the OG, and both were higher
than CG. Protein intake was similar for the OG and GG, which were significantly lower (p < 0.0005) than the CG. The growth rate (GR) of the rats, albumin levels, and total levels of serum protein were comparable
for all groups. Hematocrit (p < 0.04) and hemoglobin (p < 0.03) for the OG and GG were less than the CG. Although the OG and GG demonstrated reduced hematocrit and hemoglobin, both
types of soy were utilized in a way similar to casein. This result suggests that the protein quality of soy is parallel to
the standard protein casein in terms of growth promotion but not hematological indicators. 相似文献
11.
Wheaten pocket-type flat breads were baked from refined flours fortified to contain iron from ferrous sulphate (FeSO4), hydrogen-reduced elemental iron (Fe) or sodium iron ethylenediaminetetraacetic acid (NaFeEDTA). Individual and group visual detection thresholds were determined by the 3-alternative forced choice (3-AFC) test according to the American Society of Testing and Materials (ASTM) method E-1432. The group visual detection thresholds of FeSO4, NaFeEDTA and Fe in bread were established in samples baked from the corresponding wheat flours fortified with 69.46, 236.82 and 304.97 mg iron/kg flour, respectively. Sensory testing showed that iron-fortified pocket-type flat breads were similar (P < 0.01) to regular bread when baked from flours formulated to contain iron at levels lower by 25% than the group visual detection thresholds of Fe and FeSO4 and two 25% increments lower than the threshold of NaFeEDTA. These findings indicate that iron-fortified pocket-type flat breads, which are sensorially similar to regular bread, baked from flours that contained 52.1, 133.22 and 228.73 mg iron/kg flour as FeSO4, NaFeEDTA and Fe, would provide the segment of the population at the highest risk of iron-deficiency anaemia, specifically women of childbearing age, with 91%, 207% and 346% of their recommended daily intakes for iron, respectively. 相似文献
12.
Paśko P Zagrodzki P Bartoń H Chłopicka J Gorinstein S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):333-338
The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined
in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p < 0.01] and activity of alkaline phosphatase [20%, p < 0.05], and increased triglycerides level [86%, p < 0.01]. The analysis of blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol
[26%, p < 0.05], LDL [57%, p < 0.008] and triglycerides [11%, p < 0.05] when compared to the control group. Quinoa seeds also significantly reduced the level of glucose [10%, p < 0.01] and plasma total protein level [16%, p < 0.001]. Fructose significantly decreased HDL [15%, p < 0.05] level in control group but when the quinoa seeds were added into the diet the decrease of HDL level was inhibited.
Quinoa seeds did not prevent any adverse effect of increasing triglyceride level caused by fructose. It was shown in this
study that quinoa seeds can reduce most of the adverse effects exerted by fructose on lipid profile and glucose level. 相似文献
13.
Effect of L-arginine Oral Supplementation on Response to Myocardial Infarction in Hypercholesterolemic and Hypertensive Rats 总被引:1,自引:1,他引:0
Verónica Piñeiro Alicia Ortiz-Moreno Rosalva Mora-Escobedo María Dolores Hernández-Navarro Guillermo Ceballos-Reyes Germán Chamorro-Cevallos 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):31-37
The well known metabolic functions of L-arginine have been recently increased with the discovery of its role as the substrate
for the synthesis of nitric oxide (NO), which has emerged as an endogenous signaling molecule with potential therapeutic implications
for cardiovascular disease. Steady-state levels of NO are derived in part from dietary sources. It has been reported that
supplementation of L-arginine reduces atherosclerosis in rabbits and reduces the arterial pressure in hypertensive rats. Therefore,
we investigated the effect of L-arginine supplementation using a group of induced hypercholesterolemic rats and a group of
spontaneously hypertensive rats; the infarcted area in cardiac tissue of both groups was measured during the response to myocardial
infarction in the ischemia-reperfusion model. Hypercholesterolemic rats supplemented with 170 mg kg−1 of L-arginine showed a significant (P ≤ 0.05) reduction in total cholesterol (25.2%) and LDL (27.8%). Spontaneously hypertensive rats supplemented with L-arginine
presented a significant reduction (20.3%) in mean blood pressure (P ≤ 0.05). The index infarcted area / total heart area, in both: hypercholesterolemic and hypertensive rats supplemented with
L-arginine, showed a significant 36% and 29% of cardio protection (P ≤ 0.05) effect, respectively. Dietary supplementation with L-arginine may represent a potentially novel nutritional strategy
for the treatment of cardiovascular disease. 相似文献
14.
Doolaege EH Raes K De Vos F Verhé R De Smet S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):196-202
The absorption, distribution and elimination of carnosic acid, the main antioxidant found in rosemary was studied, in vivo, in rats. Therefore, carnosic acid was administrated in a single dose, intravenously (20.5 ± 4.2 mg/kg) and orally (64.3 ± 5.8 mg/kg),
to four and nine rats, respectively. Blood samples were collected at different time points, and plasma concentrations of carnosic
acid were determined using LC-MS. Furthermore, total collection of urine and feces was done during 4 h and 24 h for the intravenous
and oral administrations, respectively. After euthanizing the rats, intestinal content, liver and muscle tissue were sampled
to determine carnosic acid concentrations. The bioavailability of carnosic acid, after 360 min, was 40.1%. Traces of carnosic
acid were found in the rats intestinal content, liver and muscle tissue of abdomen and legs. The recovery of carnosic acid
in the feces, 24 h after oral administration, was 15.6 ± 8.2%. Carnosic acid is absorbed into the bloodstream after oral administration
in rats and is therefore bioavailable. It was found that carnosic acid in vivo is present in its free form and that its main elimination route is the fecal route. 相似文献
15.
16.
Shiro Nakano Hideo Takekoshi Masuo Nakano 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):25-30
Pregnancy anemia and pregnancy-induced hypertension (PIH) are common and potentially dangerous disorder in human pregnancy,
and nutritional status of pregnant women is one of the leading causes. Chlorella contains large quantities of folate, vitamin
B-12 and iron, and can help improve anemia and hypertensive disorder. Our objective was to investigate the preventive effects
of Chlorella supplement on pregnancy anemia and PIH in Japanese pregnant women. A total of 70 pregnant women were placed into
the control group (n = 38) or the Chlorella group (n = 32). The subjects in the Chlorella group were supplemented daily from 12th–18th wk of gestation until delivery with 6 g
of Chlorella supplement. The proportion of anemic (hemoglobin level < 11 g/dL) subjects in the Chlorella group were significantly
lower compared with the control group at the second and third trimesters. Additionally, in the Chlorella group, the incidences
of proteinuria and edema, signs of PIH, were significantly lower during the third trimester. These results suggest that Chlorella
supplementation significantly reduces the risk of pregnancy associated anemia, proteinuria and edema. Chlorella supplement
may be useful as a resource of natural folate, vitamin B-12 and iron for pregnant women. 相似文献
17.
Bioavailability to rats of iron from fortified grain amaranth 总被引:1,自引:0,他引:1
M. O. Ologunde J. B. Morris R. L. Shepard A. O. Afolabi O. L. Oke 《Plant foods for human nutrition (Dordrecht, Netherlands)》1994,45(3):191-201
In this study, fortified and unfortified grain amaranth seed flour diets and a FeSO4-fortified casein diet (used as a control) were evaluated for their iron (Fe) bioavailability. NaFeEDTA, ferrous fumarate, and FeSO4-fortified grain amaranth were fed to growing Sprague-Dawley weaning male rats. Iron intake, hemoglobin iron (HbFe) gain, Fe availability, total iron binding capacity (TIBC), serum iron, non-haem liver iron and red bloodcell volume (RBV) were determined, and the values were compared with those of the FeSO4-fortified casein diet control. Ferrous fumarate fortified diets gave consistently high values for all these parameters, compared with consistently low values for the amaranth diet without iron fortification.Relative biological values (RBVs) were 0.40, 1.55, 1.75, 1.67 and 1.00 for animals fed on an unfortified amaranth diet, and diets fortified with NaFeEDTA, ferrous fumarate, FeSO4 and casein fortified with FeSO4, respectively. Using FeSO4-fortified casein as control, ferrous fumarate gave a superior RBVs (1.75 vs. 1.00). The RBVs, of the unfortified cereal diets were 40% that of the control, perhaps suggesting low iron absorption from the amaranth cereal. Based on the results of this study, amaranth cereal can be considered an ideal food vehicle for iron fortification. The iron fortification of choice is ferrous fumarate. 相似文献
18.
Brandsch C Kappis D Weisse K Stangl GI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):410-416
Lupin protein is capable of reducing plasma lipids in hypercholesterolemic man and animals. Whether lipid-lowering properties
of lupin protein will be influenced by thermal treatment or by other nutrients has not been elucidated. In a two-factorial
study, rats were fed hypercholesterolemic diets based on high amounts of carbohydrates (HC) or fat (HF), which contained either
(20.4% of energy) untreated or thermally treated lupin protein (steam: 120 °C, 30 min) or casein as control protein. Lupin
protein lowered plasma lipid concentrations in rats fed the HF diet but not in those fed the HC diet (P < 0.05). Among rats fed the HF diet, plasma and VLDL triglyceride concentrations were lower in rats fed thermally treated
(−46% and −44%, P < 0.05) and untreated lupin protein (−47% and −46%, P < 0.05) than in those fed casein; whereas liver triglycerides were reduced only in rats fed untreated lupin protein (P < 0.05). Compared to casein, untreated lupin protein had slightly stronger cholesterol-lowering effects in plasma, LDL and
HDL (−34%, −37%, −35%; P < 0.05) than thermally treated lupin protein (−23%, −29%, −31%, P < 0.10). In conclusion, the lipid-lowering effect of lupin protein strongly depends on composition of the basal diet, and
thermal treatment is accompanied by a slight reduction of its hypocholesterolemic properties. 相似文献
19.
João C. M. Barreira José Alberto Pereira M. Beatriz P. P. Oliveira Isabel C. F. R. Ferreira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):38-43
Sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (HPLC),
by means of a refractive index (RI) detector. A solid-liquid extraction procedure was used in defatted and dried samples.
The chromatographic separation was achieved using a Eurospher 100-5 NH2 column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. All the compounds were separated in 16 min. The method was optimized and proved to be reproducible
and accurate. Generally, more than 95% of sugars were identified for both matrixes. Sugars profiles were quite homogeneous
for almond cultivars; sucrose was the main sugar (11.46 ± 0.14 in Marcona to 22.23 ± 0.59 in Ferragnes g/100 g of dried weight), followed by raffinose (0.71 ± 0.05 in Ferraduel to 2.11 ± 0.29 in Duro Italiano), glucose (0.42 ± 0.12 in Pegarinhos two seeded to 1.47 ± 0.19 in Ferragnes) and fructose (0.11 ± 0.02 in Pegarinhos two seeded to 0.59 ± 0.05 in Gloriette). Commercial cultivars proved to have higher sucrose contents, except in the case of Marcona. Nevertheless, chestnut cultivars revealed a high heterogeneity. Sucrose was the main sugar in Aveleira (22.05 ± 1.48), Judia (23.30 ± 0.83) and Longal (9.56 ± 0.91), while glucose was slightly prevalent in Boa Ventura (6.63 ± 0.49). The observed variance could serve for inter-cultivar discrimination. 相似文献
20.
A simple and rapid method for the determination of naturally occurring, potentially hazardous glycoalkaloids (GAs) in potatoes
and their products has been developed. The procedure is based on the on-line solid-phase extraction of the acetic acid extracts
from potato products and combined with liquid chromatography (LC)-mass spectrometry (MS) in a fully automated system (Symbiosis™,
Spark Holland Instruments, Emmen, The Netherlands). As sorbent material HySphere™ 18HD was used for alkaloid enrichment. GAs
were eluted with the LC gradient and directly analysed by MS. Detection of the analytes was achieved in the sensitive multiple
reaction monitoring mode using two characteristic ions (m/z 98 as a qualifier for GAs and m/z 868.3 as a quantifier for α-solanine or m/z 852.4 for α-chaconine). Typical validation data for method precision (v
k α-solanine = 5.3–6.5, v
k α-chaconine = 3.4–15.4), accuracy (average recovery of α-solanine = 84%, average recovery of α-chaconine = 87%) and linearity
over the range from 1 to 1,000 ng ml−1 (R
2 = 0.9915 for α-solanine, R
2 = 0.9939 for α-chaconine) with detection limits of 0.3 ng ml−1 for α-chaconine and 0.5 ng ml−1 for α-solanine were obtained. GA contents of commercial potato products were determined by the new on-line method and afterwards
compared with those obtained with an established high-performance LC routine procedure. Better performance of the on-line
procedure was obvious from the standard deviations of both methods. Other advantages included a strong reduction of overall
analysis time, human intervention and solvent consumption as well as waste production. The time required for the on-line analysis
was 5 min, which would allow processing of almost 100 samples in 8 h. 相似文献