Biological control of weeds in European crops: recent achievements and future work

Approaches to the biological control of weeds in arable crops and integration of biological weed control with other methods of weed management are broadly discussed. Various types of integrative approaches to biological control of weeds in crops have been studied within the framework of a concerted European Research Programme (COST‐816). During the period 1994–99, some 25 institutions from 16 countries have concentrated on five target weed complexes. Some major scientific achievements of COST‐816 are: (i) combination of the pathogen Ascochyta caulina with an isolated phytotoxin produced by this fungus to control Chenopodium album in maize and sugar beet; (ii) the elaboration and preliminary field application of a system management approach using the weed:pathogen system Senecio vulgaris:Puccinia lagenophorae to reduce the competitiveness of the weed by inducing and stimulating a disease epidemic; (iii) combination of underseeded green cover with the application of spores of Stagonospora convolvuli to control Convolvulus species in maize; (iv) assessment of the response of different provenances of Amaranthus spp. to infection by Alternaria alternata and Trematophoma lignicola, the development of formulation and delivery techniques and a field survey of native insect species to control Amaranthus spp. in sugar beet and maize; (v) isolation of strains of different Fusarium spp. that infect all the economically important Orobanche spp. and development of novel, storable formulations using mycelia from liquid culture. Although no practical control has yet been reached for any of the five target weeds, potential solutions have been clearly identified. Two major routes may be followed in future work. The first is a technological approach focusing on a single, highly destructive disease cycle of the control agent and optimizing the efficacy and specificity of the agent. The second is an ecological approach based on a better understanding of the interactions among the crop, the weed, the natural antagonist and the environment, which must be managed in order to maximize the spread and impact of an indigenous antagonist on the weed.     

我国农田杂草治理技术的发展

我国稻、麦、玉米、大豆及棉花等主要农作物农田杂草有580种,属于危害重而又难于防除的有17种,受杂草危害较重的农田有3 580万hm²,年平均受草害减产12.3％～16.5％(加权平均)。自1980年以来,全国化学除草面积以年增200万hm²的速度扩大,农田化学除草面积从20世纪70年代早期不到100万hm²扩大到近年来6 000万hm²。本文概述了我国农田主要作物杂草的发生危害、化学除草剂应用现状及其综合防治进展,并提出今后杂草防除的设想与建议。     

Competition of sorghum cultivars and densities with Japanese millet (Echinochloa esculenta)

Field studies were conducted at two locations in southern Queensland, Australia during the 2003–2004 and 2004–2005 growing seasons to determine the differential competitiveness of sorghum (Sorghum bicolor L. Moench) cultivars and crop densities against weeds and the sorghum yield loss due to weeds. Weed competition was investigated by growing sorghum in the presence or absence of a model grass weed, Japanese millet (Echinochloa esculenta). The correlation analyses showed that the early growth traits (height, shoot biomass, and daily growth rate of the shoot biomass) of sorghum adversely affected the height, biomass, and seed production of millet, as measured at maturity. “MR Goldrush” and “Bonus MR” were the most competitive cultivars, resulting in reduced weed biomass, weed density, and weed seed production. The density of sorghum also had a significant effect on the crop's ability to compete with millet. When compared to the density of 4.5 plants per m², sorghum that was planted at 7.5 plants per m² suppressed the density, biomass, and seed production of millet by 22%, 27% and 38%, respectively. Millet caused a significant yield loss in comparison with the weed‐free plots. The combined weed‐suppressive effects of the competitive cultivars, such as MR Goldrush, and high crop densities minimized the yield losses from the weeds. These results indicate that sorghum competition against grass weeds can be improved by choosing competitive cultivars and by using a high crop density of >7.5 plants per m². These non‐chemical options should be included in an integrated weed management program for better weed management, particularly where the control options are limited by the evolution of herbicide resistance.     

Effects of weed harrowing and undersown clover on weed growth and spring cereal yield

Weed competition and nutrient scarcity often restrict organic cereal production, especially where the availability of livestock manure is limited. While harrowing of annual weeds and legume cover crops can be used, these methods are both executed in early spring and may hinder each other. Two cycles of a 2‐year crop rotation were carried out in south‐east Norway (60°42′N, 10°51′E, altitude 250 m) with weed harrowing and undersown cover crops (WHCC) at two fertiliser rates (40 and 100 kg nitrogen ha^?1). The effect of the WHCC treatments was measured by weed density and species, weed biomass, changes in weed seedbank and grain yield. The weed density depended on the interaction between WHCC, fertiliser and year. On average, pre‐emergence weed harrowing reduced weed density by 32% and weed biomass by 49%, while pre‐ and post‐emergence weed harrowing reduced weed density by 59% and weed biomass by 67% compared with the untreated control. Spergula arvensis became more abundant at low rather than at high fertiliser rates. On average, white clover cover crop sown after pre‐emergence weed harrowing resulted in the highest yields for both oat (+12.1%) and wheat (+16.4%) compared with the untreated control. Despite differences in weed population density and biomass among WHCC treatments within years, the weed biomass, weed density and seedbank increased for all WHCC treatments over the 4‐year period. More research is required into improving the efficacy of mechanical and cultural weed suppression methods that organic systems rely on.     

Development of chemical weed control and integrated weed management in China

More than 200 species of weeds are infesting main crop fields in China, among which approximately 30 species are major weeds causing great crop yield losses. About 35.8 million hectares of crop fields are heavily infested by weeds and the annual reduction of crop yields is 12.3–16.5% (weighted average). Along with rural economic development, approximately 50% of the main crop fields undergo herbicide application. Chemical weed control has changed cultural practices to save weeding labor in rice, wheat, maize, soybeans and cotton. At the same time, continuous use of the same herbicides has caused weed shift problems and weed resistance to herbicides. Consequently, integrated weed management in main crops is being developed.     

Effect of anaerobic soil disinfestation amendment type and C:N ratio on Cyperus esculentus tuber sprouting,growth and reproduction

Anaerobic soil disinfestation (ASD) is a cultural technique primarily targeted for control of soilborne plant pathogens, but can also impact weed propagules. A repeated pot study was conducted to evaluate ASD treatment impact on sprouting and growth of introduced Cyperus esculentus (yellow nutsedge) tubers using dry molasses‐based and wheat bran‐based amendment mixtures at four carbon‐to‐nitrogen (C:N) ratios (from 10:1 to 40:1) and compared with a non‐amended control. The mean percentage of sprouted tubers recovered after ASD treatment was lower for wheat bran‐based (42%) than dry molasses‐based (65%) amendments, and tuber production was 1.6‐fold higher in dry molasses‐based than wheat bran‐based treatments. The highest percentage of sprouted tubers (79%) and the highest mean production of large tubers (threefold higher than wheat bran‐based and 1.7‐fold higher than molasses‐based amendments) were observed for the non‐amended control. Tuber sprouting was significantly lower from all ASD treatments (regardless of amendment C:N ratio) compared with the non‐amended control at a 15 cm burial depth. New tuber production was lowest at C:N ratios of 10:1 and 20:1 and more than twofold higher in the non‐amended control. Wheat bran‐based amendments reduced above‐ground C. esculentus biomass compared with the non‐amended control and ASD treatments with molasses‐based amendments, and reduced below‐ground biomass compared with molasses‐based amendments. Above‐ground biomass was highest at amendment C:N ratio of 10:1, and below‐ground biomass was highest at amendment C:N ratio of 40:1 and the non‐amended control. ASD treatment with wheat bran‐based amendments at lower C:N ratios reduced tuber sprouting and reproduction compared with the non‐amended control, but not at rates high enough to use as a primary weed management tactic.     

Crop competitive ability contributes to herbicide performance in sweet corn

Crop variety effects on herbicide performance is not well characterised, particularly for sweet corn, a crop that varies greatly among hybrids in competitive ability with weeds. Field studies were used to determine the effects of crop competitive ability on season‐long herbicide performance in sweet corn. Two sethoxydim‐tolerant sweet corn hybrids were grown in the presence of Panicum miliaceum and plots were treated post‐emergence with a range of sethoxydim doses. Significant differences in height, leaf area index and intercepted light were observed between hybrids near anthesis. Across a range of sub‐lethal herbicide doses, the denser canopy hybrid Rocker suppressed P. miliaceum shoot biomass and fecundity to a greater extent than the hybrid Cahill. Yield of sweet corn improved to the level of the weed‐free control with increasing sethoxydim dose. The indirect effect of herbicide dose on crop yield, mediated through P. miliaceum biomass reduction, was significant for all of the Cahill’s yield traits but not Rocker. These results indicate that a less competitive hybrid requires relatively more weed suppression by the herbicide to not only reduce weed growth and seed production, but also to maintain yield. Sweet corn competitive ability consistently influences season‐long herbicide performance.     

Reduced weed growth with different paving constructions

The recent phaseout of herbicide use on public pavements in Flanders has triggered the development of alternative weed control strategies. Besides the search for effective non‐chemical curative methods, there is also a need for strategies that prevent or reduce weed growth on pavements. In this study a paving experiment was set up under a rain shelter to investigate the effects of four construction factors on weed growth: joint filling material, joint width, organic pollution of the joint filling material and type of bedding layer. Paving mini‐plots were oversown with a mixture of dominant, hard‐to‐control weed species found on pavements. The inhibitory effect on weeds was determined by examining initial weed density and weed coverage over a 2‐year period. More weed growth was found in pavings with wide joints and organically polluted joint filling materials. High permeability of the bedding layer resulted in higher weed cover. The coarse‐grained filling materials and the sodium silicate‐enriched sand Dansand^® were associated with less weed cover than the fine‐grained filling materials. Our results show there is potential for preventing weed growth using suitable paving materials and appropriate high‐standard construction and maintenance of pavements.     

Competition between sugarbeet and Sinapis arvensis and Chenopodium album, as affected by timing of nitrogen fertilization

The effects of timing of N fertilization (early, standard or late) on competition between two sugarbeet cultivars (Ritmo, semi-prostrate; and Rizor, erect) and two weeds ( Sinapis arvensis and Chenopodium album ) were studied over 2 years at Viterbo. In both years, time of N fertilization did not influence biomass, yield and yield quality of the weed-free crop, but early N fertilization gave higher crop biomass reduction in the presence of S. arvensis and lower crop biomass reduction in the presence of C. album . Root and sucrose yield responded to competition in the same way as biomass. However, percentage reductions were higher, as both weeds affected harvest index. The two cultivars showed the same response to competition. At the weed densities studied, crop competitive ability was favoured by late N fertilization in the presence of S. arvensis and by early N fertilization in the presence of C. album . Crop competition only reduced seed production by C. album but lowered seed germination of both weeds.     

Comparison of crop and weed height,for potential differentiation of weed patches at harvest

Weeds and weed control are major production costs in global agriculture, with increasing challenges associated with herbicide‐based management because of concerns with chemical residue and herbicide resistance. Non‐chemical weed management may address these challenges but requires the ability to differentiate weeds from crops. Harvest is an ideal opportunity for the differentiation of weeds that grow taller than the crop, however, the ability to differentiate late‐season weeds from the crop is unknown. Weed mapping enables farmers to locate weed patches, evaluate the success of previous weed management strategies, and assist with planning for future herbicide applications. The aim of this study was to determine whether weed patches could be differentiated from the crop plants, based on height differences. Field surveys were carried out before crop harvest in 2018 and 2019, where a total of 86 and 105 weedy patches were manually assessed respectively. The results of this study demonstrated that across the 191 assessed weedy patches, in 97% of patches with Avena fatua (wild oat) plants, 86% with Raphanus raphanistrum (wild radish) plants and 92% with Sonchus oleraceus L. (sow thistles) plants it was possible to distinguish the weeds taller than the 95% of the crop plants. Future work should be dedicated to the assessment of the ability of remote sensing methods such as Light Detection and Ranging to detect and map late‐season weed species based on the results from this study on crop and weed height differences.     

Potential uses of small unmanned aircraft systems (UAS) in weed research

Small unmanned aerial systems (UAS) with cameras have not been adopted in weed research, but offer low‐cost sensing with high flexibility in terms of spatial resolution. A small rotary‐wing UAS was tested as part of a search for an inexpensive, user‐friendly and reliable aircraft for practical applications in UAS imagery weed research. In two experiments with post‐emergence weed harrowing in barley, the crop resistance parameter, which reflects the crop response to harrowing, was unaffected by image capture altitude in the range from 1 to 50 m. This corresponded to image spatial resolution in the range from 0.3 to 17.1 mm per pixel. This finding is important because spatial resolution is inversely related to sensing capacity. We captured 20 plots comprising a total of about 0.2 ha in one image at 50 m altitude without losing information about the cultivation impacts on vegetation compared with ground truth data. UAS imagery also gave excellent results in logarithmic sprayer experiments in oilseed rape, where we captured 37 m long plots in each image from an altitude of 35 m. Furthermore, perennial weeds could be mapped from UAS images. These first experiences with a small rotary‐wing UAS show that it is relatively easy to integrate as a tool in weed research and offers great potential for site‐specific weed management.     

Effectiveness of water permeable joint filling materials for weed prevention in paved areas

The recent phase‐out of herbicide use on public pavements in Flanders has triggered the development of alternative strategies for weed prevention and control. In this study, growth chamber experiments investigated the ability of various water permeable joint filling materials for pavements to prevent weed growth. Joint fillers included in the tests comprised five innovative (iron slag sand, polymeric bound sand and three sodium silicate enriched fillers) and eight standard joint fillers (four fine materials, for example, sea sand, white sand, sandstone and fine limestone, and four coarse materials based on porphyry and limestone). Their ability to suppress weeds was investigated by examining seedling emergence and biomass production of seven test species in pure or organically polluted (5%, 10%, 20%, 40% and 80% compost by volume) filler substrate. Selected test species were dominant, hard‐to‐control weeds found on pavements. Seedling emergence and weed biomass were lowest in iron slag sand, polymeric bound sand and most sodium silicate enriched fillers, irrespective of pollution level or test species. Within standard joint fillers, pure white sand, sandstone and the coarse materials also reduced biomass, but their inhibitory effect dropped quickly once organically polluted, in contrast to fine limestone and sea sand for which weed suppression lasted longer (up to 40% compost by volume). Weed suppression of joint fillers was species specific. Our results show that there is potential for preventing weed growth using fillers that prevent the growth of a wide spectrum of plant species over a long period.     

Influence of soil moisture on the competitive ability and seed dormancy of Sinapis arvensis in spring wheat

Two experiments were carried out, one in 1995 and one in 1997, to investigate the competitive abilities of two spring wheat cultivars with Sinapis arvensis L. The spring wheat cultivars (Baldus and Canon) of contrasting growth habit were grown with and without S. arvensis under two different moisture regimes (10% and 70% of field capacity). In 1995, S. arvensis was found to be less competitive when subjected to moisture stress, resulting in smaller wheat yield losses in dry soil than in moist soil. In both years, seed production of S. arvensis was reduced by competition and moisture stress, and the seeds produced by plants that had been grown in drier soil were small and had negligible dormancy. Hence, in dry conditions, the competitiveness of S. arvensis and its potential to produce persistent seed may be reduced. Some differences between the two wheat cultivars were evident: cv. Baldus was more competitive against S. arvensis than cv. Canon. This could be attributed to differences in canopy structure.     

Crop residue retention suppresses seedling emergence and biomass of winter and summer Australian weed species

Crop residue retention could affect the emergence and biomass of weeds in different ways. A summer and winter pot study was conducted to evaluate the effect of different amounts of sorghum and wheat residue on the emergence and biomass of 12 summer and winter Australian weeds. The equivalent amount of sorghum residue to 0, 1, 2, 4 and 6 t/ha was used in the summer study and winter weed seeds were covered with wheat residue equivalent to the amount of 0, 1, 2, 4 and 8 t/ha in the winter study. The emergence and biomass of Amaranthus retroflexus and Echinochloa colona was not affected by sorghum residue treatment. For other summer weeds, the use of the 6 t/ha sorghum residue treatment resulted in 59–94% reductions in biomass compared to no‐sorghum residue retention. Similarly, the application of 8 t/ha wheat residue in the winter study resulted in a reduced biomass of 15–100% compared to no‐crop residue treatment. The results demonstrated the high potential of using crop residues in eco‐friendly weed management strategies, such as harvest weed seed control tactics.