共查询到20条相似文献,搜索用时 31 毫秒
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Laura Elizabeth Joan Huson Edith Authié Alain Fran?ois Boulangé James Phillip Dean Goldring Theresa Helen Taillefer Coetzer 《Veterinary research》2009,40(6)
The protozoan parasite Trypanosoma congolense is the main causative agent of livestock trypanosomosis. Congopain, the major lysosomal cysteine proteinase of T. congolense, contributes to disease pathogenesis, and antibody-mediated inhibition of this enzyme may contribute to mechanisms of trypanotolerance. The potential of different adjuvants to facilitate the production of antibodies that would inhibit congopain activity was evaluated in the present study. Rabbits were immunised with the recombinant catalytic domain of congopain (C2), either without adjuvant, with Freund’s adjuvant or complexed with bovine or rabbit α2-macroglobulin (α2M). The antibodies were assessed for inhibition of congopain activity. Rabbits immunised with C2 alone produced barely detectable anti-C2 antibody levels and these antibodies had no effect on recombinant C2 or native congopain activity. Rabbits immunised with C2 and Freund’s adjuvant produced the highest levels of anti-C2 antibodies. These antibodies either inhibited C2 and native congopain activity to a small degree, or enhanced their activity, depending on time of production after initial immunisation. Rabbits receiving C2-α2M complexes produced moderate levels of anti-C2 antibodies and these antibodies consistently showed the best inhibition of C2 and native congopain activity of all the antibodies, with maximum inhibition of 65%. Results of this study suggest that antibodies inhibiting congopain activity could be raised in livestock with a congopain catalytic domain-α2M complex. This approach improves the effectiveness of the antigen as an anti-disease vaccine candidate for African trypanosomosis. 相似文献
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《Veterinary parasitology》1986,21(2):141
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R. G. Board 《British poultry science》1975,16(1):89-91
1. Open pores were a notable feature of the shell of hens' eggs which were naturally devoid of cuticle.
2. In contrast with normal eggs, the surface of the cuticle‐less shell was irregularly contoured and, in many instances, deeply fissured. 相似文献
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Hudgens E Tompkins D Boyd P Lunney JK Horohov D Baldwin CL 《Veterinary immunology and immunopathology》2011,141(3-4):317-321
This report describes the cloning and characterization of expressed gene sequences of bovine, equine, and swine CXCL9 from RNA obtained from peripheral blood mononuclear cells (PBMC) and other tissues. The bovine coding region was 378 nucleotides in length, while the equine and swine coding regions were 381 nucleotides. Mapping showed that all three sequences were coded for in four exons in the genome, as are the human and mouse genes. The bovine, equine, and swine coding regions shared 83%, 86%, and 84% homology with human CXCL9, respectively, and all three were 74% homologous with mouse CXCL9. Cladogram comparison of the nucleotide sequences of CXCL9 showed that the bovine, equine and swine sequences were more closely related to one another than to either the human or the mouse sequences. However, the human sequence was more closely related to them than it was to the mouse sequence. These relationships were preserved when the deduced amino acid sequences were evaluated and all sequences showed conservation of the characteristic four cysteines. This work sets the stage for further work with these molecules; an integral goal of the U.S. Veterinary Immune Reagent Network is to develop reagents for investigating diseases in livestock species, poultry, and fish. 相似文献
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Jump performances of Ctenocephalides canis and Ctenocephalides felis felis have been measured and compared on unfed young imagos. The mean length of the C. felis felis jump was 19.9+/-9.1cm; minimum jump was 2cm, and the maximum was one 48cm. The C. canis jump was significantly longer (30.4+/-9.1cm; from 3 to 50cm). For height jump evaluation, grey plastic cylindric tubes measuring 9cm in diameter were used. Their height was increasing from 1 to 30cm by 1cm. Groups of 10 fleas of the same species were deposited on the base of the tube. The number of fleas which succeeded in jumping above the tube was recorded. The mean height jump carried out by 50% of fleas was calculated after linearisation of the curves: it was 15.5 and 13.2cm for C. canis and C. felis, respectively. The highest jump was 25 for C. canis and 17cm for C. felis. 相似文献
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Even though occidental food production and control systems guarantee a reasonable level of safety for consumers, western countries are facing a growing phenomenon of immigration from all over the world. With this enrichment and the shuffling of cultures and customs in the same places, even alimentary habits undergo an unavoidable evolution, also influencing the commercial trends of society. The growing demand for ethnic products, often of uncertain provenance, composition and manufacturing, has motivated the appearance of illegal trade and markets or improper local productions besides the legal ones, implying a rise in hygienic risks for the consumers. These underground realities, together with the unfilled gap that still exists between different cultures and customs cause significant difficulties in carrying out efficient controls to guarantee sanitary safety for consumers. For this reason, the risks associated with this increasing globalization must be faced with proper information and a decisive formative strategy must be divided, in order to allow the operator of the whole system and control employees to be acquainted about different raw materials, manufacturing systems, alimentary habits, languages for communication and commercial dynamics which stand beyond the new alimentary trends. 相似文献
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The cause of haematuria often can be surmised based on a horse's signalment, recent history, and clinical signs that may accompany haematuria. The site, if not the cause, of haemorrhage can sometimes be revealed by examining that portion of the urinary tract palpable per rectum, by endoscopically examining the urethra and bladder, or by ultrasonographically examining the kidneys. When cystolithiasis or urethrolithiasis is determined to be the cause of haematuria, further examination is indicated to identify disease of one or both kidneys that might have precipitated formation of the urolith. Contrast-enhanced, cross-sectional imaging of the abdomen of small equids might be of value when a vascular anomaly of a kidney is suspected. Neoplastic cells can sometimes be found in the urine of horses suffering from neoplasia of the bladder or in peritoneal fluid of horses suffering from renal neoplasia. When a kidney is determined to be the source of haemorrhage, urine can be obtained for bacterial culture and cytological examination by passing tubing through the biopsy port of an endoscope into the ureter draining the kidney in question. Histological examination of tissue obtained by biopsy of the kidney or the mucosa of the bladder may be indicated when neoplasia of one of these organs is suspected. Immunohistochemical examination of endoscopically obtained tissue from a cystic carcinoma can identify whether the neoplastic cells express COX-2 receptors, thereby determining if treating the horse with a COX-2 selective NSAID might be effective. 相似文献
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The present review aims to compile the currently available literature since 1936 according the sources of infection of the Q fever pathogen (Coxiella (C.) burnetii) as well as the transmission from animal to man and also from human to human. In terms of quality and validity, the existing publications were reviewed systematically. For this purpose, firstly a structured literature search was carried out using various databases and search engines supplemented by a manual literature search. For critical appraisal, 1444 relevant publications were identified for the moment and evaluated. A total of 73 publications describing a transmission of C. burnetii from animals to man or a human-to-human transmission were discovered. The identified publications are 29 case series, two case reports, 21 cohort studies and 21 case-control studies. With regard to the sources of infection, 25 publications describing the transmission of C. burnetii from sheep to humans could be identified. 相似文献
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Adoligbe M. C. Amaveda M. A. Adido M. S. Djimenou D. Farougou S. 《Tropical animal health and production》2020,52(6):3015-3025
Tropical Animal Health and Production - An exploratory survey was conducted to understand breeding practices and breeding objectives as well as the constraints as a first step towards developing... 相似文献
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Eighty dogs from a locality west of the Concei??o Lagoon, in the Santa Catarina Island, Florianópolis Municipality, State of Santa Catarina, Brazil, were examined for dirofilariasis by thick smear and by modified Knott's method. Twelve of them (15%) were positive, five only by Knott's, two only by thick smear and five by both methods. Positive dogs were observed in several parts of the locality, indicating that the parasite is widespread. Prevalence was significantly higher in older animals. Size, sex, hair length, place of sleeping and hour of blood collection did not significantly influence the results. Forty dogs of the Military Police kennel, at S?o José Municipality, Santa Catarina State, were all negative. Studies on the transmission and control of dirofilariasis in the region should be developed. 相似文献