Immunoglobulin A multiple myeloma with cutaneous involvement in a dog

An 8-year-old rottweiler, diagnosed with multiple myeloma and multiple sites of cutaneous involvement, was treated with chemotherapy and radiation therapy. The diagnostic criteria for canine multiple myeloma, limitations of diagnostic testing for light chain proteinuria in dogs, and the role of radiation therapy in multiple myeloma patients is discussed.     

MALIGNANT TRANSFORMATION OF SOLITARY SPINAL OSTEOCHONDROMA IN TWO MATURE DOGS

Canine osteochondroma is an uncommon bony tumor that arises in skeletally immature animals. Consequently, clinical signs typically occur in young dogs as a result of impingement of normal structures by the tumor. Radiographically, osteochondromas are benign in appearance. They are well circumscribed and cause no bony lysis nor periosteal proliferation. Osteochondromas may occur in two forms; solitary or multiple. Although histology and biologic behavior are identical, when in the multiple form the condition has been termed multiple cartilaginous exostoses. Malignant transformation of multiple cartilaginous exostoses has been reported in three mature dogs. We report two dogs with malignant transformation of solitary spinal osteochondromas. Both underwent transformation to osteosarcoma. Despite the benign radiographic appearance of osteochondromas and multiple cartilaginous exostoses, clinical signs should alert the clinician to the possibility of malignant transformation.     

海河平原区棉花饲用黑麦复种方式及水肥利用效率分析

2011年10月-2014年10月连续3年在河北省任县安排了定位试验,通过饲用黑麦不同播期与棉花构建几种棉草复种茬口搭配方式,从产量、经济效益、水分利用效率和养分利用效率以及对土壤肥力影响等方面综合分析棉花饲用黑麦复种的可行性及复种方式。结果表明,棉花可与饲用黑麦复种,为降低饲用黑麦对棉花的影响,饲用黑麦可适当推迟到10月20日播种,次年4月底收获,效益可提高5470.1元·hm^-2。棉花饲用黑麦复种与棉花单作相比,平均水分利用率提高16.49%;水分经济利用效率提高56.54%;并且土壤有机质平均增长幅度提高5.71%,土壤含盐量平均降低幅度达到23.72%,土壤改良效果明显;是适用于该区域的高效复种模式。     

IMAGING DIAGNOSIS—DISSEMINATED PERITONEAL LEIOMYOMATOSIS IN A DOG

A 17‐month‐old male Labrador retriever presented for evaluation of an abdominal mass felt during abdominal palpation. Multiple variably sized cystic masses were identified on sonographic and radiographic images. Exploratory laparotomy revealed multiple peritoneal masses that exhibited atypical contractions and lacked an identifiable organ of origin. Histology and immunohistochemistry of multiple surgically excised masses was consistent with benign tumors of smooth muscle origin (leiomyomas). The presence of multiple peritoneal leiomyomas in this dog is consistent with disseminated peritoneal leiomyomatosis. Two years after diagnosis and multiple surgical interventions, continual insidious enlargement of leiomyomas was identified on ultrasound and CT.     

发展湖南省农区草业的商榷

本文根据湖南省农业生产现状,论述了发展农区草业的重要性和有利条件。作者认为:湖南省具有丰富的绿肥、饲草资源。发展农区草业必须走草种多用途、多种类、多途径、多效益的道路。     

油葵—饲用谷子复种栽培技术规程的编制

根据多年的试验总结,研究油葵—饲用谷子复种栽培的要求,以及油葵栽培、饲用谷子栽培等技术要求,明确了该技术规程的具体措施与技术指标,旨在提供油葵—饲用谷子复种栽培规范化、标准化、产业化、市场化的技术支撑。     

病毒灭毛剂等四种杀虫剂的室内药效比较试验

用病毒灭毛剂等四种杀虫剂进行室内灭杀草原毛虫药效试验。结果表明 ,病毒灭毛剂稀释 1 0 0倍的灭效为 1 0 0 %、5 0 0倍的灭效为 95 % ;杀灭灵稀释 1 0 0倍时灭效为 91 .5 % ,来福灵稀释 1 0 0倍的灭效为87.5 %。病毒灭毛剂灭效最好 ,敌杀死次之 ,杀灭灵和来福灵均较差。病毒灭毛剂是一种安全、经济、有效的生物药剂 ,充分利用和推广病毒灭毛剂对草地植被和环境保护具有重要意义     

畜禽饲粮配合新思路:多配方平行设计

多配方平行设计是另一类畜禽饲粮配合方法,本研究旨在将这种方法应用于动物饲料配方。采用不同组合的多配方平行设计同时配合了生长猪7个不同阶段猪群的饲粮,比较与分析饲粮单位价格、原料用量和养分含量。通过这种设计方法产生的49个生长猪饲粮配方的能量、钙、磷、粗蛋白质、氨基酸、总氮和矿物质的养分含量,都达到或超过美国国家研究委员会(2012)提出的猪营养需要的推荐量,也满足最低成本的要求。实例表明,多配方平行设计能同时配合多个畜禽饲粮,并能在一个大的范围内选到更好的配方,应用于动物饲料配方设计具有实际可行性。     

鸭新城疫病毒和流感病毒多重RT-PCR鉴别诊断技术的建立和应用

对同样以引起鸭产蛋下降为主要特征的鸭流感病毒（AIV）分离株和鸭新城疫病毒（NDV）YH99V分离株，通过设计特异性引物和优化体系反应条件，建立了一种早期快速鉴别诊断鸭AIV和鸭NDV的单项RT—PCR方法和多重RT—PCR方法，AIV的扩增片段大小为483bp，NDV的扩增片段为310bp，电泳快速，易于区分。敏感性试验表明，该方法比HA敏感100倍以上；对AIV、YH99V、IBV、IBDV及正常鸡胚尿囊液的混合液检测显示，多重RT—PCR法具有很强的特异性。对鸭病毒性产蛋下降征侯群送检病料的检测证明，该方法能有效鉴别AIv和／或NDV单独感染或混合感染，阳性检出率明显高于HA方法。     

Effects of stage and number of tumours on prognosis of dogs with cutaneous mast cell tumours

Between 1997 and 1999, 280 dogs with mast cell tumours were identified, of which 59 (21 per cent) had multiple tumours. Follow-up data for survival analysis were available for 145 dogs with single tumours and 50 dogs with multiple tumours. There was no significant difference between the survival times of the two groups; the survival rates after 12 and 24 months were 88 per cent and 83 per cent, respectively, for the dogs with single tumours, and 86 per cent at both intervals for the dogs with multiple tumours. Eight of the dogs with single tumours had lymph node metastases (stage II disease) and these dogs had a median survival time of 431 days, whereas the 50 dogs with multiple tumours (classified as stage III disease) and the dogs with single tumours (classified as stage I disease) had not reached their median survival times. Golden retrievers appeared to be predisposed to developing multiple tumours in the population studied, with an odds ratio of 3.8. This study found no evidence that dogs with multiple tumours had different survival times than those with single tumours, although there was evidence that the presence of lymph node metastasis generally carried a poorer prognosis.     

Multiple Corpora Lutea in Dromedary Camels: Are They Associated with Multiple Pregnancies?

The aim of this study was to investigate whether multiple corpora lutea (CL) in dromedary camels were associated with multiple pregnancies. Three experiments were carried out. In the first experiment, reproductive tracts of 124 slaughtered female camels were examined for the incidence of multiple CL and the pregnancy status. In the second experiment, uteri and ovaries of 50 females were examined by ultrasound between the fourth and fifteenth weeks of pregnancy to estimate the number and location of the embryos/fetuses and the number of associated CL. In the third experiment, 20 females were mated and the pregnant animals were followed weekly to estimate the pattern of embryonic and fetal growth. At the slaughterhouse, multiple CL were detected in 44.4% of the pregnant animals. By ultrasonography, the incidence of twinning and triplets was 52%. The incidence significantly (P < 0.05) decreased from the fourth to the thirteenth week of pregnancy. Intrauterine growth retardation (IUGR) was observed in all animals of twins and triplets. Only one viable fetus could be detected after the ninth week of pregnancy in each pregnant animal. Both conceptuses died in one animal. In conclusion, multiple CL in dromedary camels are usually associated with multiple pregnancies. IUGR occurred in all animals of twins/triplets, with only one fetus surviving after the ninth week of pregnancy.     

Multiple dermoid sinuses of type Vb and IIIb on the head of a Saint Bernard dog

Dermoid sinus, a congenital malformation of neural tube development, has been reported in humans and several animal species including dogs. It is typically found in the dorsal midline and commonly occurs in the Rhodesian Ridgeback breed. A case of multiple dermoid sinuses in the fronto-occipital region is described. An 11-month-old, intact female Saint Bernard dog was presented with a 2 day history of discharge from a large irregular subcutaneous mass in the fronto-occipital region. The dog was otherwise healthy. The dog had two circular skin lesions (approximately 4 × 4 and 4 × 2 cm diameter) surrounded by multiple irregular elevated masses. The masses had multiple small openings on the skin surface with tufts of hair protruding from the apertures. The masses were surgically removed, and the diagnosis of multiple dermoid sinuses was confirmed by histological examination. Histopathological examination showed multiple, variably sized, spherical to tubular cysts expanding the dermis and subcutis. Cysts were filled with hair shafts and lamellar keratin and were lined by a stratified squamous epithelium. Sebaceous and apocrine gland adnexal structures were also observed. To the best of our knowledge, this is the first reported case of multiple dermoid sinuses of two different types in the head of a Saint Bernard dog.     

Inheritance of multiple births in cattleHéridité des naissances gemellaires chez les bovinsVererbung von mehrlingsgeburten beim rindvieh

Records on approximately one million calvings were examined for presence of a gene with major effect on the frequency of multiple births. The records were summarized by parity of the cow and registration number of the cow's sire, and an overall age-corrected frequency of multiple births computed for daughters of each sire.The distribution of 876 progeny groups with more than 100 calvings showed no sign of discontinuity, and the same was true for 120 progeny groups with more than 1000 calvings. The daughters of one particular bull had 9.95% multiple births in about 6000 calvings. This bull left more than 80 sons which were used for artificial insemination. The sons ranged from less than one to more than 10% multiple births among their daughters, but no segregation was observed.Heritability estimates (on the binomial scale) ranged from 0.006 in parity 1 to about 0.04 in parities 3–5.Genetic correlations between incidence of multiple births in first and subsequent parities were estimated at 0.52–0.64, while those among parities 2–5 were from 0.70 to 0.84.     

家蚕微粒子病母蛾检查五次抽样方案研究

为了保证蚕种质量 ,提出了家蚕微粒子病母蛾抽样检查的五次抽样方案。理论计算、计算机模拟和实际检验的结果证明 ,通过适当的设计 ,可以找到与现行二次抽样方案抽样特征曲线相近的五次抽样方案 ,即具有相近的生产方风险和使用方风险 ,而使平均镜检次数明显减少。五次抽样方案的操作步骤相对繁琐 ,但可以采用计算机抽样检查软件弥补     

"Blended" online technology: maximizing instructor reach

This article describes the development and implementation of a small animal nutrition course for veterinary technicians using 'blended' online technology and delivery at multiple sites. Student contact was accomplished through both synchronous and asynchronous Internet platforms. A summary of student evaluations is provided. Application of the process described here may be appropriate where specific content expertise is available in one location and desired in multiple locations. Unique features of the course include (a) course and instructor provided by industry partner, (b) synchronous and asynchronous blended online elements, and (c) multiple schools engaged in merged, simultaneous sessions.     

CHARACTERIZATION OF MULTIPLE ACQUIRED PORTOSYSTEMIC SHUNTS USING TRANSPLENIC PORTAL SCINTIGRAPHY

We describe the scintigraphic patterns observed in 14 patients with confirmed multiple portosystemic shunts imaged via transplenic portal scintigraphy. Parameters evaluated included presence of multiple anomalous vessels, presence of hepatofugal flow caudal to spleen, and/or to cranial margin of the kidneys, slow absorption resulting in longer spleen to heart transit time, and presence of biphasic or fragmented bolus. Twenty‐eight additional patients, 14 with a confirmed single portocaval and 14 with a portoazygos shunt, were used for comparison. Nine of 14 (64.3%) patients with multiple shunts had multiple vessels, five (35.7%) had a biphasic bolus, 13 (92.9%) had hepatofugal flow caudal to the cranial margin of the kidneys. In all single portocaval shunts, a single anomalous vessel was identified. None had hepatofugal flow caudal to the border of the kidneys. Among portoazygos shunts, 4/14 (28.6%) had flow caudal to the injection site. Six portoazygos and one portocaval shunts had biphasic bolus. Median transit time from spleen to heart was significantly longer (1.9 s) in patients with multiple shunts than in patients with a portocaval shunt (1.0 s), but not in patients with a portoazygos shunt (1.3 s). Although a distinct plexus of anomalous vessels is not detected in all patients with multiple shunts imaged using transplenic portal scintigraphy, findings of hepatofugal flow caudal to the margin of the kidneys, and longer transit time compared with single portocaval shunts were characteristic. Flow caudal to the splenic injection site but cranial to the kidneys and biphasic bolus can also be seen with a single congenital shunt.     

Multiple trichoepitheliomas in an alpaca (Lama pacos).

A 13-yr-old male Alpaca (Lama pacos) presented with multiple ovoid, well-circumscribed, nonulcerated intradermal masses. Individual masses measured 1-4 cm in diameter, and the overlying skin was alopecic. Several of the masses were surgically removed and evaluated microscopically. Histopathologic evaluation demonstrated multiple dermal cysts lined by neoplastic follicular epithelium. The cysts were filled with multiple layers of lamellar keratin and lined by abortive inner and outer root sheaths exhibiting cellular atypia, supporting the diagnosis of trichoepitheliomas. No additional treatment was initiated, and the alpaca continues to do well.     

多年生黑麦草高频丛生芽增殖培养体系的研究

选取多年生黑麦草(Lolium Perenne L.)的3个优良品种:里加(Regal)、贝尔勒(Belle)、马拉松(Marathon)作为植物材料,以无菌苗的茎尖为外植体,对多年生黑麦草丛生芽的诱导、增殖进行研究,以其建立多年生黑麦草丛生芽高频增殖体系,并在此基础上确定丛生芽离体培养的最适筛选剂及其浓度。结果表明:当2,4-D和6-BA浓度分别为0.5 mg·L^-1和2.0 mg·L^-1时有利于茎尖诱导产生丛生芽;丛生芽增殖的最适6-BA浓度为2.0 mg·L^-1;继代培养时间过长对丛生芽块的生长不利;基因型对丛生芽的诱导和增殖有很大影响;适宜的生根培养基为1/2 MS+NAA 0.1 mg·L^-1。此外,还确定了丛生芽的最适筛选剂为G418和Hyg,其浓度分别为150 mg·L^-1和75 mg·L^-1。从而,建立了一种诱导率和增殖率较高并且实验周期短的丛生芽高频增殖培养体系,并进一步探讨了建立多年生黑麦草遗传转化受体系统的必要条件。     

不同剂量环磷酰胺对小鼠免疫功能抑制作用的研究

旨在观察不同剂量及不同给药方式下环磷酰胺对健康小鼠的免疫抑制作用,探索建立小鼠环磷酰胺免疫抑制模型的方法。采用腹腔注射法给予小鼠不同剂量的环磷酰胺,通过测定免疫器官指数,血清谷丙转氨酶、谷草转氨酶、尿素氮、肌酐含量,血液白细胞数量,脾淋巴细胞转化率等指标,评价环磷酰胺对小鼠机体免疫力以及肝肾功能的影响。结果表明,连续7 d分别给予小鼠腹腔注射10、20、30、40 mg/（kg·BW）的环磷酰胺和单次注射150 mg/（kg·BW）的环磷酰胺,均可对小鼠产生不同程度的免疫抑制作用,并引起肝肾功能相关指标的变化;低剂量多次注射环磷酰胺产生的免疫抑制效果优于高剂量单次注射,且在低剂量多次注射时,免疫抑制效果随注射剂量的升高而增强。连续7d给予健康小鼠腹腔注射30~40 mg/（kg·BW）的环磷酰胺,可建立小鼠免疫抑制模型。     

Progression of a solitary, malignant cutaneous plasma-cell tumour to multiple myeloma in a cat

An 11‐year‐old male domestic shorthair cat was examined because of a soft‐tissue mass on the left tarsus previously diagnosed as a malignant extramedullary plasmacytoma. Findings of further diagnostic tests carried out to evaluate the patient for multiple myeloma were negative. Five months later, the cat developed clinical evidence of multiple myeloma based on positive Bence Jones proteinuria, monoclonal gammopathy and circulating atypical plasma cells. This case represents an unusual presentation for this disease and documents progression of an extramedullary plasmacytoma to multiple myeloma in the cat.