Dissolved and Soil Organic Carbon after Long‐Term Conventional and No‐Tillage Sorghum Cropping

Abstract

Distribution of dissolved (DOC) and soil organic carbon (SOC) with depth may indicate soil and crop‐management effects on subsurface soil C sequestration. The objectives of this study were to investigate impacts of conventional tillage (CT), no tillage (NT), and cropping sequence on the depth distribution of DOC, SOC, and total nitrogen (N) for a silty clay loam soil after 20 years of continuous sorghum cropping. Conventional tillage consisted of disking, chiseling, ridging, and residue incorporation into soil, while residues remained on the soil surface for NT. Soil was sampled from six depth intervals ranging from 0 to 105 cm. Tillage effects on DOC and total N were primarily observed at 0–5 cm, whereas cropping sequence effects were observed to 55 cm. Soil organic carbon (C) was higher under NT than CT at 0–5 cm but higher under CT for subsurface soils. Dissolved organic C, SOC, and total N were 37, 36, and 66%, respectively, greater under NT than CT at 0–5 cm, and 171, 659, and 837% greater at 0–5 than 80–105 cm. The DOC decreased with each depth increment and averaged 18% higher under a sorghum–wheat–soybean rotation than a continuous sorghum monoculture. Both SOC and total N were higher for sorghum–wheat–soybean than continuous sorghum from 0–55 cm. Conventional tillage increased SOC and DOC in subsurface soils for intensive crop rotations, indicating that assessment of C in subsurface soils may be important for determining effects of tillage practices and crop rotations on soil C sequestration.     

华北山前平原典型厚包气带硝态氮分布累积规律

包气带是连接大气层和含水层水分和养分转换的纽带,也是农田NO_3~–-N分布和累积的重要场所和向含水层淋失的通道,因此研究包气带土壤中NO_3~–-N的分布累积规律对防止地下水NO_3~–-N污染至关重要。本文以中国科学院栾城试验站典型的厚包气带为对象,在无施肥处理(N0)和施氮肥600 kg/(hm~2·a)(N600)两种处理的多年试验田中,利用Geoprobe获取0~10.5 m深度土壤样品,研究厚包气带NO_3~–-N垂向分布、累积规律,并分析其影响因素。结果表明:N0中NO_3~–-N基本保持不变,长年施氮肥600 kg/(hm~2·a)使得NO_3~–-N淋溶至10.5 m,并在深层包气带中形成累积,累积的峰值由土壤的质地和含水量决定;NO_3~–-N的分布和累积主要受水分运移、土壤质地和反硝化作用影响。     

Carbon and nitrogen mineralization after maize harvest between and within maize rows: a microcosm study using 13C natural abundance

The sequestration of carbon in soil is not completely understood, and quantitative information about the rates of soil organic carbon (SOC) turnover could improve understanding. We analyzed the effects of the uneven distribution of crop residues after harvest of silage maize on C and N losses (CO₂‐C, dissolved organic carbon (DOC) and nitrogen (DON), and NO₃^–) from a Haplic Phaeozem and on the occurrence of priming effects induced by the decomposition of accumulated maize residues. Soil columns were taken from a continuous maize (since 1961) field after harvest i) between maize stalk rows (M_bare), ii) within the maize rows including a standing maize stalk (M_stalk), and iii) from a continuous rye (since 1878) field after tillage (rye stalk and roots were mixed into the Ap horizon). The soil columns were incubated for 230 days at 8 °C with an irrigation rate of 2 mm 10^–2 M CaCl₂ per day. Natural ¹³C abundance was used to distinguish between maize‐derived C (in SOC and maize residues) and older C originating from former C₃ vegetation. The uneven distribution of maize residues resulted in a considerably increased heterotrophic activity within the maize rows as compared with soil between seed rows. Cumulative CO₂ production was 53.1 g CO₂‐C m^–2 for M_stalk and 23.3 g CO₂‐C m^–2 for M_bare. The contribution of maize‐derived C to the total CO₂ emission was 83 % (M_stalk) and 67 % (M_bare). Calculated as difference between CO₂‐C release from M_stalk and M_bare, 19 % of the maize residues (roots and stalk) in M_stalk were mineralized during the incubation period. There was no or only a marginal effect of the accumulation of maize residues in M_stalk on leaching of DOC, DON, and NO₃^–. Total DOC and DON leaching amounted to 2.5 g C m^–2 and 0.16 g N m^–2 for M_stalk and to 2.1 g C m^–2 and 0.12 g N m^–2 for M_bare. The contribution of maize‐derived C to DOC leaching was about 25 % for M_stalk and M_bare. Nitrate leaching amounted to 3.9 g NO₃^–‐N m^–2 for M_stalk and to 3.5 g NO₃^–‐N m^–2 for M_bare. There was no priming effect induced by the decomposition of fresh maize residues with respect to CO₂ or DOC production from indigenous soil organic carbon derived from C₃ vegetation.     

好氧条件下两种水稻土的氮素总转化速率比较

Although to date individual gross N transformations could be quantified by ~(15)N tracing method and models,studies are still limited in paddy soil.An incubation experiment was conducted using topsoil(0-20 cm) and subsoil(20-60 cm) of two paddy soils,alkaline and clay(AC) soil and neutral and silt loam(NSL) soil,to investigate gross N transformation rates.Soil samples were labeled with either ~(15)NH4_NO_3 or NH_4~(15)NO_3,and then incubated at 25 °C for 168 h at 60%water-holding capacity.The gross N mineralization(recalcitrant and labile organic N mineralization) rates in AC soil were 1.6 to 3.3 times higher than that in NSL soil,and the gross N nitrification(autotrophic and heterotrophic nitrification) rates in AC soil were 2.4 to 4.4 times higher than those in NSL soil.Although gross NO_3~- consumption(i.e.,NO_3~- immobilization and dissimilatory NO_3~- reduction to NH_4~+ rates increased with increasing gross nitrification rates,the measured net nitrification rate in AC soil was approximately 2.0 to 5.1 times higher than that in NSL soil.These showed that high NO_3~- production capacity of alkaline paddy soil should be a cause for concern because an accumulation of NO_3~- can increase the risk of NO_3~- loss through leaching and denitrification.     

Denitrification enzyme activity and potential of subsoils under grazed grasslands assayed by membrane inlet mass spectrometer

The importance of subsoil denitrification on the fate of agriculturally derived nitrate (NO₃) leached to groundwater is crucial for budgeting N in an ecosystem and for identifying areas where the risk of excess NO₃ is reduced. However, the high atmospheric background of di-nitrogen (N₂) causes difficulties in assessing denitrification enzyme activity (DEA) and denitrification potential (DP) in soils directly. Here, we apply Membrane Inlet Mass Spectrometry (MIMS) technique to investigate indirectly DEA and DP in soils by measuring N₂/Ar ratio changes in headspace water over soil. Soils were collected from 0-10, 15-25 and 60-70 cm depths of a grazed ryegrass and grass-clover. The samples were amended with helium-flushed deionized water containing ranges of NO₃ and carbon (glucose-C) and were incubated for six hours in the dark at 21 °C. The peaks for N₂/Ar ratio, declined with increasing soil depth, indicating a reduced substrate requirements to initiate DEA en-masse (15-30 mg NO₃-N alone or with 60-120 mg glucose-C, kg⁻¹ soil). The dissolved N₂O concentrations were very small (0.004-0.269 μg N kg⁻¹ soil) but responded well to the added N and C, showing a reduction in DEA with soil depth. In three separate studies, only subsoils were incubated for 3 days at 12 °C with 20-30 mg NO₃-N ± 40-60 mg glucose-C, kg⁻¹ soil. Denitrification capacity (DC, NO₃ only treatment) was not statistically different to the control (no amendment) within a land use (0.03-0.05 vs. 0.07-0.22 mg N kg⁻¹ soil d⁻¹), the highest being in ryegrass subsoils receiving groundwater. The DP was significantly (P < 0.0001) higher in subsoils under ryegrass than under grass-clover (0.50-0.71 vs. 1.15 mg N kg⁻¹ soil d⁻¹). The rates of DP (NO₃ + glucose-C) increased significantly (P < 0.0001) in unsaturated and saturated subsoils (0.92 and 2.19 mg N kg⁻¹ soil d⁻¹, respectively) of grass-clover, due to the higher reductive state resulting from the 10 day pre-incubation. Available C accelerated denitrification in soils and superseded the temporary elevation in oxidative state due to NO₃ addition. The substrates load differences between the land uses regulated the degree of denitrification rates. Results suggest that both dissolved N₂O measured by gas chromatography and N₂/Ar ratio measured by MIMS to indirectly determine DEA, and the latter to quantify total DC/DP in soils can be used. However, interference of oxygen in the MIMS system should be considered if available C is added or is naturally elevated in soil or groundwater.     

Water and Fertilizer Nitrogen Management to Minimize Nitrate Pollution from a Cropped Soil in Southwestern Quebec,Canada

Nitrate-N (NO₃ ^--N) pollution of water resources is a widely recognized problem. Water and nitrogen fertilizer are the two most important factors affecting NO₃ ^--N movement to surface and groundwater. Field trials were conducted from 1998 to 2000 growing seasons to investigate the combined impacts of water table management (WTM) and N fertilization rate on NO₃ ^--N concentration in the soil profile and in drain discharge. There were two water table treatments: free drainage (FD) with open drains at a 1.0 m depth from the soil surface and subirrigation (SI) with a target water table depth of 0.6 m below the soil surface, and two N fertilizer rates: 120 kg N ha^-1 (N₁₂₀) and 200 kg N ha^-1 (N₂₀₀) in a split-plot design. Compared to FD, SI reducedNO₃ ^--N concentration in the soil by up to 50% averaged over the two N rates. Concentrations of NO₃ ^--N in drainage water fromSI plots were lower than those from FD by 55 to 73%. These findings suggest that SI can be used as a means of reducing soil NO₃ ^--N pollution and drainage water NO₃ ^--N concentrations.     

Carbon dynamics determined by natural C abundance in microcosm experiments with soils from long-term maize and rye monocultures

Understanding carbon dynamics in soil is the key to managing soil organic matter. Our objective was to quantify the carbon dynamics in microcosm experiments with soils from long-term rye and maize monocultures using natural ¹³C abundance. Microcosms with undisturbed soil columns from the surface soil (0-25 cm) and subsoil (25-50 cm) of plots cultivated with rye (C₃-plant) since 1878 and maize (C₄-plant) since 1961 with and without NPK fertilization from the long-term experiment ‘Ewiger Roggen’ in Halle, Germany, were incubated for 230 days at 8 °C and irrigated with 2 mm 10⁻² M CaCl₂ per day. Younger, C₄-derived and older, C₃-derived percentages of soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass (C_mic) and CO₂ from heterothropic respiration were determined by natural ¹³C abundance. The percentage of maize-derived carbon was highest in CO₂ (42-79%), followed by C_mic (23-46%), DOC (5-30%) and SOC (5-14%) in the surface soils and subsoils of the maize plots. The percentage of maize-derived C was higher for the NPK plot than for the unfertilized plot and higher for the surface soils than for the subsoils. Specific production rates of DOC, CO₂-C and C_mic from the maize-derived SOC were 0.06-0.08% for DOC, 1.6-2.6% for CO₂-C and 1.9-2.7% for C_mic, respectively, and specific production rates from rye-derived SOC of the continuous maize plot were 0.03-0.05% for DOC, 0.1-0.2% for CO₂-C and 0.3-0.5% for C_mic. NPK fertilization did not affect the specific production rates. Strong correlations were found between C₄-derived C_mic and C₄-derived SOC, DOC and CO₂-C (r≥0.90), whereas the relationship between C₃-derived C_mic and C₃-derived SOC, DOC and CO₂-C was not as pronounced (r≤0.67). The results stress the different importance of former (older than 40 years) and recent (younger than 40 years) litter C inputs for the formation of different C pools in the soil.     

Participation of soluble and oxidizable soil organic compounds in denitrification

Summary The role of soluble organic carbon (SOC) in denitrification in four mineral soils and one organic soil was evaluated in laboratory studies. Denitrification capacities and SOC concentrations were determined by nitrate loss from air-dried flooded soil treated with a solution containing 100 g/ml N0₃ ^–-N, while the rate of consumption was measured by Warburg manometry on 20 g air-dried soils to which 10 ml water had been added. High correlation coefficients (r > 0.93) were obtained between denitrification capacities, SOC, and oxygen consumption in the five soils. A mineral soil was amended with extracts of an organic soil. After incubating for 1 week, denitrification capacity was enhanced and SOC concentrations decreased in that soil. Extracted mineral soil had a lower denitrification capacity than an unextracted one. Decreases in concentrations of SOC were related to color change. Infrared spectra of precipitates from soil extracts indicated that absorption at wave number 1420–1440 cm -1 was also related to the color changes. It was implied that low molecular weight fulvic acid like compounds represented the SOC mineralized in denitrification, and that their supply to soil solution by solubilization of organic matter influenced the denitrification rate in the soil.     

Natural 13C abundance and soil carbon dynamics under long‐term residue retention in a no‐till maize system

Residue retention and reduced tillage are both conservation agricultural practices that may enhance soil organic carbon (SOC) stabilization in soil. We evaluated the long‐term effects of no‐till (NT) and stover retention from maize on SOC dynamics in a Rayne silt loam Typic Hapludults in Ohio. The six treatments consisted of retaining 0, 25, 50, 75, 100 and 200% of maize residues on each 3 × 3 m plot from the crop of previous year. Soil samples were obtained after 9 yrs of establishing the experiment. The whole soil (0–10 and 10–20 cm of soil depths) samples under different treatments were analysed for total C, total N, recalcitrant C (NaOCl treated sample) and ¹³C isotopic abundance (0–10 cm soil depth). Complete removal of stover for a period of 9 yrs significantly (P < 0.01) decreased soil C content (15.5 g/kg), whereas 200% of stover retention had the maximum soil C concentration (23.1 g/kg). Relative distribution of C for all the treatments in different fractions comprised of 55–58% as labile and 42–45% as recalcitrant. Retention of residue did not significantly affect total C and N concentration in 10–20 cm depth. ¹³C isotopic signature data indicated that C₄‐C (maize‐derived C) was the dominant fraction of C in the top 0–10 cm of soil layer under NT with maize‐derived C accounting for as high as 80% of the total SOC concentration. Contribution of C₄‐C or maize‐derived C was 71–84% in recalcitrant fraction in different residue retained plots. Residue management is imperative to increase SOC concentrations and long‐term agro‐ecosystem necessitates residue retention for stabilizing C in light‐textured soils.     

Dynamics of Soil Moisture,pH, Organic Carbon,and Nitrogen Under Switchgrass Cropping in a Semiarid Sandy Wasteland

Switchgrass (Panicum virgatum L.) is a perennial biofuel crop with a high production potential and suitable for growth on marginal land. This study investigates the long-term planting effect of switchgrass on the dynamics of soil moisture, pH, organic carbon (SOC), total nitrogen (TN), nitrate nitrogen (NO₃^–-N) and ammonium nitrogen (NH₄⁺-N) for soils to a depth of 90-cm in a sandy wasteland, Inner Mongolia, China. After crop harvesting in 2015, soil samples were collected from under switchgrass stands established in 2006, 2008, and 2009, native mixture, and a control that was virgin sand. Averaged across six layers, soil moisture and pH was significantly higher under the native mixture than switchgrass or virgin sand. However, SOC and TN were significantly higher under the 2006 switchgrass stand when compared with all other vegetation treatments and the control. The SOC and TN increased from 2.37 and 0.26 g kg^?1, respectively, for 2009 switchgrass stand, and to 3.21 and 0.42 g kg^?1, respectively, for 2006 switchgrass stand. Meanwhile, SOC and TN contents were 2.51 and 0.27 g kg^?1, respectively, under the native mixture. The soil beneath switchgrass and native mixture showed the highest NO₃^–-N and NH₄⁺-N, respectively. The soil moisture increased with depth while SOC, TN, and NO₃^–-N decreased. An obvious trend of increasing moisture, SOC, TN, and mineral N was observed with increasing switchgrass stand age. Thus, growing switchgrass on sandy soils can enhance SOC and TN, improve the availability of mineral N, and generate more appropriate pH conditions for this energy cropping system.     

氮沉降背景下生物炭施用对土壤有机碳组分的影响

通过18个月的盆栽试验,以杉木幼苗为研究对象,研究不同水平氮(N)沉降背景下(N0(0)、低N(40 kg/(hm2·a))和高 N(80 kg/(hm2·a))生物炭(BC)施用(B0(0)、B1(12 t/hm2)和 B2(36 t/hm2))对土壤有机碳(SOC)组分的影响.结果表明:与对照相比,单独施用BC以及...     

华北山前平原农田土壤硝态氮淋失与调控研究

本文依托中国科学院栾城农业生态系统试验站小麦-玉米一年两熟长期定位试验, 应用土钻取土和土壤溶液取样器取水的方法, 研究了不同农田管理措施下土壤硝态氮的累积变化, 计算了不同氮肥处理通过根系吸收层的硝态氮淋失通量。结果表明, 小麦-玉米生长季土壤硝态氮累积量和淋失量随着施氮量的增加显著增加, 相同氮肥水平下增施磷、钾肥增加了作物的收获氮量, 施磷肥增加的作物收获氮量最高可达123kg·hm^-2·a^-1, 施钾肥增加的作物收获氮量最高为31 kg·hm^-2·a^-1。不同灌溉水平下0~400 cm 土体累积硝态氮随着灌溉量的增加而降低, 控制灌溉(小麦季不灌水, 玉米季灌溉1 水)、非充分灌溉(小麦季灌溉2~3 水, 玉米季按需灌溉)、充分灌溉(小麦季灌溉4~5 水, 玉米季按需灌溉)各处理剖面累积硝态氮量分别为1 698 kg·hm^-2、1148 kg·hm^-2 和961 kg·hm^-2。与非充分灌溉和充分灌溉处理相比, 控制灌溉在100~200 cm 土层硝态氮累积量显著高于其他层次, 2003~2005 年间控制灌溉剖面增加的硝态氮量占施肥总量的23%; 非充分灌溉处理剖面增加的硝态氮量占施肥总量的22%; 充分灌溉处理剖面增加的硝态氮量占施肥总量的47%。免耕措施降低了作物产量, 影响土壤水的运移, 增加了硝态氮的淋失风险。根据作物所需降低氮素投入(N 200 kg·hm^-2·a^-1), 增施磷、钾肥, 控制灌溉量是减少华北山前平原地区硝态氮淋失, 保护地下水的有效措施。     

In Situ Batch Denitrification of Nitrate-Rich Groundwater Using Sawdust as a Carbon Source—Marydale, South Africa

Batch experiments were performed to denitrify groundwater using sawdust as a carbon source at Marydale, South Africa. Alkalinity, pH, electrical conductivity, nitrate, nitrite, ammonia, SO ₄ ^2? , heterotrophic plate count (HPC), dissolved organic carbon (DOC), potassium and chloride were monitored. Two soil depths, 75 to 100 and 165 to 200 cm, respectively, from the Marydale area were used as matrix material during denitrification based on contrasting chemical composition with respect to major ion composition and moisture to consider different denitrification rates for varying soil depths. Different N to C ratios were used to evaluate the denitrification efficiency and the least undesirable products, e.g., elevated SO ₄ ^2? , H₂S and other reduced compounds. DOC is directly proportional to the N to C ratio used. Nitrite was produced for most of the treatments as incomplete denitrification occurred. The incubation periods were 28 and 43 days, respectively. N to C ratios were 12.6:1, 24:1, 34:1 and 54:1. Longer incubation period and higher N to C ratio resulted in total removal of both nitrate and nitrite. The reaction was carbon-limited for lower N to C ratios. The denitrification rate was proportional to the carbon availability at any time during the experiment. There was no significant difference in denitrification using heterogeneous and homogeneous particle sizes for sawdust. Soil depth of 75–100 cm displayed a greater denitrification rate than 165–200-cm soil depth due to higher initial soil nitrate concentration. The method showed some specificity, as DOC, nitrite, nitrate, alkalinity and HPC were the only parameters that showed a change in concentration over the duration of the denitrification experiment under constant temperature and nitrogen gas atmosphere. DOC and HPC were unacceptable for domestic use, but methods such as boiling or chlorinating water can rid it of bacteria.     

减氮配施有机物质对麦田土壤性质和小麦产量的影响

探究氮肥减量配施有机物质的情况下对氮素利用状况及土壤肥力和小麦产量的影响,为我国华北平原区小麦生产中提高氮肥利用效率、实现节肥增效提供理论基础。通过田间试验,设置5个处理：不施氮肥(CK)、农民习惯施氮肥(FN)、减氮20%(80%FN)、减氮20%+生物有机肥(80%FN+OM)、减氮20%+生物炭(80%FN+BC),研究小麦生长关键期土壤容重、有机质、NO₃^-—N和土壤微生物多样性的变化,测定小麦产量并计算氮素利用效率。结果表明,土壤容重受施入有机物质影响显著,成熟期0—20,20—40 cm的80%FN+OM、80%FN+BC的土壤容重较80%FN分别下降3.83%～4.58%和2.96%～5.07%。成熟期0—40 cm的土壤有机质均以80%FN+OM最高,较其他施氮肥处理提高2.13%～18.81%。土壤NO₃^-—N受施氮肥影响显著,挑旗期80%FN+OM和80%FN+BC处理的0—40 cm土壤NO₃^-—N较高;灌浆期80%FN+BC处理的0...     

Modeling a long-term effect of rice straw incorporation on SOC content and grain yield in rice field

ABSTRACT

The long-term effects of rice straw incorporation on soil organic carbon (SOC) content and rice yield were evaluated from rice cultivation with different treatments: no rice straw (control), rice straw (RS), and rice straw compost (RSC) as a main-plots; five levels of nitrogen (N) fertilizer application: 0, 100, 150, 200, and 250 of N (kg ha^?1) as a sub-plots. The denitrification and decomposition (DNDC) model was employed to simulate changes in SOC content and rice grain yield over 35 years. Additionally, scenario analysis on continuous or discontinuous RS incorporation in rice fields was conducted using the DNDC model. The long-term results indicated that RS and RSC treatments played a crucial role in increasing grain yields by 9% and 11% due to the increased SOC contents compared to control treatment. The validated DNDC model on SOC contents and yields showed a good agreement between the observed and simulated values based on the normalized root mean square errors. The model predicted a rapid decline of SOC contents without RS incorporation. Results suggested that incorporating rice straw or amending manure to paddy soils is a preferred practice for maintaining SOC contents.     

黄土高原人工油松林土壤碳氮对短期氮添加的响应

为了更好地理解土壤碳氮元素对氮添加的响应,通过短期原位模拟氮沉降试验,揭示黄土高原子午岭人工油松(Pinus tabulaeformis Carrière)林土壤碳氮对外源氮添加的响应过程和机制。从2015—2016年设置4个氮添加水平,分别为对照(0kg/(hm~2·a),N0)、低氮(50kg/(hm~2·a),N50)、中氮(100kg/(hm~2·a),N100)和高氮(200kg/(hm~2·a),N200),研究人工油松林地不同深度土层土壤有机碳和全氮以及土壤碳氮储量对模拟氮添加的响应。结果表明:土层对土壤有机碳、全氮和碳氮储量有显著影响,上层土壤有机碳、全氮和碳氮储量显著高于下层土壤;氮添加水平对土壤有机碳、土壤碳储量影响不显著,但可显著影响土壤全氮和氮储量。此外,土壤有机碳、全氮和碳氮储量和土壤碳氮储量比受地下生物量碳氮比的影响显著。因此,短期氮添加对人工油松林地土壤碳的影响不显著,但可显著影响土壤氮,地下生物量碳氮比是影响土壤碳氮的重要因素。     

旱作地区长期小麦连作和苜蓿连作土壤剖面的矿质氮研究

对不同施肥条件下23年小麦连作地和苜蓿连作地土壤矿质氮分布和累积进行研究,探讨种植浅根系和深根系植物对硝态氮淋溶的影响。结果表明,不施肥(CK)和单施磷(P)肥,小麦和苜蓿连作地土壤硝态氮主要集中在0—60 cm土层,0—60 cm土层以下硝态氮含量变化稳定并小于2 mg/kg。氮肥、磷肥和有机肥配施(NPM)时,小麦连作地土壤硝态氮累积在20—100 cm和140—320 cm土层,年累积速率可达42.12 kg/(hm2.a);苜蓿连作土壤硝态氮主要集中在0—60 cm土层,仅在200—300 cm土层出现轻微累积,年累积速率仅为1.01 kg/(hm2.a)。在不施肥和单施磷肥下,种植小麦或苜蓿对土壤硝态氮残留量影响不显著,而氮、磷和有机肥配施时,小麦连作地土壤硝态氮残留量迅速增加,并与不施肥、单施磷肥处理有显著差异;苜蓿连作地土壤硝态氮残留量虽有少量增加,但与不施肥、单施磷肥处理无显著差异。不施肥、单施磷肥和氮、磷和有机肥配施,小麦连作、苜蓿连作地土壤剖面铵态氮含量主要在10—20 mg/kg之间波动,在土壤剖面无明显的累积现象,铵态氮残留量受施肥和作物种类的影响不显著。     

Effects of freeze–thaw cycles and the soil water content on carbon and nitrogen changes in different soil types of Heilongjiang Province,China

To reveal the influence of freeze–thaw cycles (FTCs) on soil carbon and nitrogen changes, six typical soils in Northeast China were selected as the research objects to conduct a FTC simulation test in an artificial climate chamber. Three soil volumetric water contents (10%, 20%, 30%) and eight FTCs (0, 2, 4, 6, 8, 10, 15, 20) were set. The results showed that the soil organic carbon (SOC) and microbial biomass carbon (MBC) contents of different soil types under the FTCs initially exhibited a downward and then an upward trend, while the dissolved organic carbon (DOC) content exhibited an upward and then a downward trend. Otherwise, the fourth and sixth FTCs were the key points of change. The SOC, MBC and DOC contents in paddy fields were higher than those in dry fields, showing upward and then downward trends spatially from northeast to southwest. The SOC and MBC contents in each soil type were the highest at the 20% water content, and the DOC content gradually increased with increasing water content. The ammonium nitrogen (NH₄⁺-N) content in different soil types at different water contents under the FTCs showed an upward trend first, then a downward trend and finally an upward trend. The NH₄⁺-N content in paddy fields was higher than that in dry fields. The nitrate nitrogen (NO₃^‒-N) content showed a downward trend first, then an upward trend and finally a downward trend. The NO₃^‒-N content in dry fields was higher than that in paddy fields. The NH₄⁺-N contents in the three soil types on the Sanjiang Plain were significantly higher than those on the Songnen Plain. The NH₄⁺-N and NO₃^‒-N contents showed upward trends with increasing water content, but the differences were not significant. The results have implications for the study of different types of soils and provide references for research on the mechanism of soil carbon and nitrogen transformation in typical farming areas in Northeast China.     

不同施肥方式下土壤氨氧化细菌的群落特征

为了研究长期定位施肥对棕壤中氨氧化细菌（ammonia-oxidizing bacteria,AOB）种群结构多样性和垂直分布特征的影响,本研究采用化学分析、荧光定量PCR（qPCR）和变性梯度凝胶电泳（PCR-DGGE）技术,针对沈阳农业大学试验区不同施肥方式（不施肥、低量无机氮肥、高量无机氮肥、无机氮肥与有机肥配施）下不同土壤深度（0~20 cm、20~40 cm、40~60 cm）的土壤理化性质、AOB丰度及种群多样性进行分析,比较不同施肥方式对土壤AOB种群的影响。结果显示,与不施肥相比,施肥会降低土壤pH,增加土壤铵态氮（70.5%~939.21%）和硝态氮（253.20%~625.48%）含量。随土壤深度增加,土壤pH升高,铵态氮和硝态氮含量除低量无机氮肥处理外,多呈降低趋势。土壤增施氮肥可提高AOB丰度,降低总细菌丰度。其中,0~20 cm土层中AOB丰度较高,且高量无机氮肥处理的AOB数量最高,为9.65×10⁵拷贝数·g^-1（干土）。DGGE图谱分析显示,不同处理下,AOB群落结构多样性指数存在明显差异（P<0.05）,各多样性指数均在表层（0~20 cm）最高,增施氮肥则显著降低AOB的多样性。聚类分析表明,4个施肥处理中,高量无机氮肥处理聚为一类,其他处理则因土壤深度不同而异;3个土壤深度中,除不施肥处理外,所有施肥处理均表现为0~20 cm、20~40 cm土层发生聚类,40~60 cm则明显与其他两层分开。冗余梯度分析（RDA）显示,硝态氮（P=0.027）是造成影响AOB群落结构差异的主要原因。上述研究结果表明,长期定位施肥土壤AOB的数量和群落结构多样性受施肥方式显著影响,并表现出明显的垂直分布特征。与无机氮肥相比,有机无机配施处理有助于改善土壤pH,维持不同土壤深度下AOB群落结构多样性。     

Contribution of sorption,DOC transport and microbial interactions to the 14C age of a soil organic carbon profile: Insights from a calibrated process model

Profiles of soil organic carbon (SOC) are often characterized by a steep increase of ¹⁴C age with depth, often leading to subsoil ¹⁴C ages of more than 1000 years. These observations have generally been reproduced in SOC models by introducing a SOC pool that decomposes on the time-scale of millennia. The overemphasis of chemical recalcitrance as the major factor for the persistence of SOC was able to provide a mechanistic justification for these very low decomposition rates. The emerging view on SOC persistence, however, stresses that apart from molecular structure a multitude of mechanisms can lead to the long-term persistence of organic carbon in soils. These mechanisms, however, have not been incorporated into most models. Consequently, we developed the SOC profile model COMISSION which simulates vertically resolved SOC concentrations based on representations of microbial interactions, sorption to minerals, and vertical transport. We calibrated COMISSION using published concentrations of SOC, microbial biomass and mineral-associated OC (MOC), and in addition, ¹⁴C contents of SOC and MOC of a Haplic Podzol profile in North-Eastern Bavaria, Germany. In order to elucidate the contribution of the implemented processes to the ¹⁴C age in different parts of the profile, we performed model-experiments in which we switched off the limitation of SOC decomposition by microbes, sorptive stabilization on soil minerals, and dissolved OC (DOC) transport. By splitting all model pools into directly litter-derived carbon and microbe-derived organic carbon, we investigated the contribution of repeated microbial recycling to ¹⁴C ages throughout the profile. The model-experiments for this site lead to the following implications: Without rejuvenation by DOC transport, SOC in the subsoil would be on average 1700 ¹⁴C years older. Across the profile, SOC from microbial recycling is on average 1400 ¹⁴C years older than litter-derived SOC. Without microbial limitation of depolymerization, SOC in the subsoil would be on average 610 ¹⁴C years younger. Sorptive stabilization is responsible for relatively high ¹⁴C ages in the topsoil. The model-experiments further indicate that the high SOC concentrations in the Bh horizon are caused by the interplay between sorptive stabilization and microbial dynamics. Overall, the model-experiments demonstrate that the high ¹⁴C ages are not solely caused by slow turnover of a single pool, but that the increase of ¹⁴C ages along a soil profile up to ages >1000 years is the result of different mechanisms contributing to the overall persistence of SOC. The dominant reasons for the persistence of SOC are stabilization processes, followed by repeated microbial processing of SOC.