Impact of fertilization on drought response in the medicinal herb Bupleurum chinense DC.: Growth and saikosaponin production

Bupleurum chinense DC. is a plant with high medicinal value. Its roots have been used in Chinese medicine for at least 2000 years. Environmental stress has been used as a strategy to optimize yield of important compounds in other crops. The objective of this pot study was to investigate the combined effects of fertilizer and water-stress on total saikosaponin a (SSa) and saikosaponin d (SSd) yield from B. chinense. The 2 × 3 factoral design included two levels of water-stress and three fertilizer amounts. The results showed that mild water-stress significantly increased the SSa and SSd content in B. chinense roots, but decreased root biomass. Total SSa and SSd yield were lower in the water-stressed treatment compared to the well-watered treatment. There was significant interaction between the water and fertilizer treatments and the negative effect of water-stress on total SSa and SSd yield could be partly mitigated through the application of N and P fertilizer. In conclusion, results from this study show that the application of proper amounts of fertilizer are important for medicinal plant production in semi-arid and arid regions, and that it is possible to increase total SSa and SSd yield through the combined use of fertilizer and properly timed exposure to water-stress.     

Fertilizer and planting strategies to increase biomass and improve root morphology in the natural rubber producer Taraxacum brevicorniculatum

Taraxacum brevicorniculatum produces high-quality natural rubber in its roots and could be developed as an alternative commercial source of this valuable raw material. However, current wild type accessions have a low biomass and branched roots that make them difficult to harvest. We set out to determine the optimum fertilizer and spacing requirements for T. brevicorniculatum plants in greenhouse and field trials, aiming to increase root biomass and reduce branching thus maximizing rubber yield and reducing losses during harvesting. Our preliminary data from greenhouse trials show that fertilizers containing calcium and magnesium in addition to NPK (nitrogen, phosphorus and potassium) increase root biomass by 25% compared to standard NPK fertilizer plus boron, and by 15% compared to NPK plus calcium. Fertilizers with a low N:P ratio increased the root biomass in greenhouse and field trials. Dense sowing (5-10 cm between plants) inhibits root branching and leads to the formation of single taproots that can be harvested easily. We therefore provisionally recommend the application of low N:P ratio NPK fertilizer supplemented with calcium and magnesium to closely-spaced plants in order to achieve the greatest increase in plant biomass per hectare.     

Evaluation of tuber-bearingSolanum species for nitrogen use efficiency and biomass partitioning

Modern potato cultivars (Solanum tuberosum L.) require high rates of fertilizer nitrogen (N). This practice is costly and can pose a serious threat to surface and groundwater. Previous evaluation of wild potato germplasm demonstrated the existence of species capable of producing high total biomass under low N conditions, with the ability to make maximum use of added N. Therefore, a two-year field experiment was conducted in 1994 and 1995 to investigate the response of selected wild potato accessions and their hybrids with the haploid USW551 (USW) to low and high N environments. The haploid USW and cultivars Russet Burbank, Red Norland, and Russet Norkotah were also included in the study. Uniform propagules and seedlings from the variousSolanum species were transplanted to a Hubbard loamy sand (Udic Haploboroll) at Becker, Minn. and were subjected to two N treatments: 0 and 225 kg N ha^-1. At harvest, total dry biomass of wild and hybrid potato germplasm was equal to or higher than that of the cultivars. However, cultivar biomass partitioning was 1% to roots, 15% to shoots, 0% to fruits, and 84% to tubers, whereas wild potato species partitioned 18% to roots plus nontuberized stolons, 52% to shoots, 23% to fruits, and only 7% to tubers. Hybrids were intermediate, allocating 9% of their biomass to roots plus nontuberized stolons, 39% to shoots, 14% to fruits, and 38% to tubers. Nitrogen use efficiencies for many of the species and crosses were comparable to that for Russet Burbank and greater than those for Red Norland and Russet Norkotah. Of the wild species tested,S. chacoense accessions had the highest biomass accumulation and N uptake efficiencies and may be the best source of germplasm for improving NUE in a potato breeding program.     

Long-term effects of mulch,fertilization and tillage on cassava grown in sandy soils in northern Colombia

Cassava (Manihot esculenta Crantz) is often cultivated in sandy soils that are very low in nutrients and organic matter. Under such conditions, yields often decline when the crop is grown successively without fertilizer application. An 8-year on-farm trial was conducted on sandy soils in northern Colombia to study effects of (1) surface mulching with residues of the grass Panicum maximum, (2) moderate applications of N, P and K fertilizer and (3) tillage on crop productivity, root quality and soil properties. Mulch applications significantly increased root and top biomass, increased root dry matter content while reducing its yearly variation, and decreased root HCN, particularly in the absence of fertilizer. Mulch applications also significantly reduced soil temperatures within the top 20 cm and increased soil organic carbon, K, P, Ca and Mg. Without mulch, soil pH decreased over the years. Annual applications of 21 kg ha⁻¹ P resulted in a build-up of soil P, whereas no build-up of soil K was observed with applications of 41 kg K ha⁻¹. The application of N, P and K fertilizer significantly increased root and top biomass and reduced root HCN, especially if no mulch was applied. Where both mulch and fertilizer applications were absent, root yield and top biomass declined over the years. Tillage, except when combined with fertilizer application, had no effect on root yield, top biomass, root dry matter or HCN contents. Neither were effects of tillage observed in any of the studied soil parameters. The trial indicated that, to sustain cassava productivity in poor sandy soils, applications of plant mulch and/or chemical fertilizer are highly desirable.     

不同施肥量对降香黄檀苗木生长和叶片养分状况的影响

以半年生降香黄檀苗木为研究对象,利用普罗丹水溶性复合肥(N-P2O5-K2O:20-20-20),按照施肥量(按N用量计算)0、400、800、1200、1600 mg/株设置5个施肥量处理开展指数施肥盆栽试验,探讨施肥量对降香黄檀苗木生长以及叶片养分含量的影响,运用临界浓度法确定降香黄檀苗木的适宜施肥量,为规模化培育...     

局部施磷对橡胶树根系及植株生长的影响

采用盆栽分根试验，设计了5个局部施磷区比例，研究了局部等量施磷对橡胶树幼苗根系及植株生长的影响。结果表明：局部施磷区占盆栽土壤1/4以上时，可促进根系的发生，根系总长度及根系总表面积增加，但植株叶片磷含量及株高无显著影响；局部施磷区占盆栽土壤1/4以下时，根系数量、总长度、总表面积、生物量、叶片磷含量及株高均显著下降。     

Effects of Organic and Inorganic Nitrogen on the Growth and Production of Domoic Acid by Pseudo-nitzschia multiseries and P. australis (Bacillariophyceae) in Culture

Over the last century, human activities have altered the global nitrogen cycle, and anthropogenic inputs of both inorganic and organic nitrogen species have increased around the world, causing significant changes to the functioning of aquatic ecosystems. The increasing frequency of Pseudo-nitzschia spp. in estuarine and coastal waters reinforces the need to understand better the environmental control of its growth and domoic acid (DA) production. Here, we document Pseudo-nitzschia spp. growth and toxicity on a large set of inorganic and organic nitrogen (nitrate, ammonium, urea, glutamate, glutamine, arginine and taurine). Our study focused on two species isolated from European coastal waters: P. multiseries CCL70 and P. australis PNC1. The nitrogen sources induced broad differences between the two species with respect to growth rate, biomass and cellular DA, but no specific variation could be attributed to any of the inorganic or organic nitrogen substrates. Enrichment with ammonium resulted in an enhanced growth rate and cell yield, whereas glutamate did not support the growth of P. multiseries. Arginine, glutamine and taurine enabled good growth of P. australis, but without toxin production. The highest DA content was produced when P. multiseries grew with urea and P. australis grew with glutamate. For both species, growth rate was not correlated with DA content but more toxin was produced when the nitrogen source could not sustain a high biomass. A significant negative correlation was found between cell biomass and DA content in P. australis. This study shows that Pseudo-nitzschia can readily utilize organic nitrogen in the form of amino acids, and confirms that both inorganic and organic nitrogen affect growth and DA production. Our results contribute to our understanding of the ecophysiology of Pseudo-nitzschia spp. and may help to predict toxic events in the natural environment.     

Reducing rice field algae and cyanobacteria abundance by altering phosphorus fertilizer applications

In California’s water-seeded rice systems, algal/cyanobacterial biomass can be a problem during rice establishment and can lead to yield reductions. Laboratory, enclosure, and field-scale experiments were established to evaluate the effects of fertilizer P management on algal/cyanobacterial growth. Two field-scale experiments evaluated the response of algal/cyanobacterial growth to three P management strategies: conventional surface applied, incorporated into the soil, and delaying P applications by 30 days. Results from these experiments indicated rice fields that received conventional surface-applied P fertilizer had 4–8 times more algal/cyanobacterial biomass and 3–11 times higher concentrations of soluble reactive phosphate (SRP) than those in which P fertilizer was incorporated or delayed. Laboratory experiments evaluated the ability of field water to support growth of Nostoc spongiaeforme. Results indicate that water from the incorporated or delayed P application fields was P limited for N. spongiaeforme growth. Water from the surface-applied fields was not P limited. Enclosure experiments evaluated the effects of delayed P applications on algal/cyanobacterial biomass and rice yields. Algal/cyanobacterial cover and biomass increased in enclosures which received added P. Soluble reactive phosphate concentrations were also significantly greater in these enclosures. Delaying the application by up to 28 days did not reduce rice yields in the enclosures. One management implication is that reducing SRP concentrations early in the season in rice field water will result in reduced algal/cyanobacterial biomass. Strategies to reduce water SRP include incorporating fertilizer P or delaying the P application by up to 30 days.     

CARBON, NITROGEN, PHOSPHORUS AND SULPHUR IN HERBAGE PLANT ROOTS

C. N, P and S were determined in the roots of white- and red-clover, lucerne, perennial ryegrass, cocksfoot and timothy, taken at intervals over a period of several months, and in roots of ryegrass receiving four levels of fertilizer N. The results indicated that decomposition of The root material would result in The mineralization of N in substantial amounts from white clover, in smaller amounts from red clover and in negligible amounts from lucerne. The roots of grasses would tend to immobilize soil N, even when They bad received moderate applications of fertilizer N. The C:P ratios suggested that the legume roots would neither mineralize nor immobilize inorganic pbospbate but that the grass roots would induce immobilization. S was likely to be mineralized in appreciable amounts from roots of white– and red–clover, but not firom roots of lucerne or the grasses.     

Comparison of the requirements and utilization of nitrogen by genotypes of sorghum (Sorghum bicolor (L.) Moench), and nodulating and non-nodulating groundnut (Arachis hypogaea L.)

Nitrogen requirements and utilization of mineral nitrogen (N) by sorghum and groundnut were compared. At the maximum N use level, sorghum genotypes showed greater N use efficiency (120 kg biomass/kg N harvested) than groundnut genotypes (36 kg biomass/kg N harvested). Using a non-nodulating groundnut genotype (Non-nod) or sorghum as controls for soil N uptake, the amounts of N₂ fixed by the nodulated groundnut genotypes were estimated to be 183–190 kg N/ha. Nitrogen fertilization increased harvest index and percentage N translocated to seeds in sorghum genotypes, but decreased harvest index and had variable effects on percentage N translocated to seed in groundnut genotypes. Leaf nitrate reductase activity (NRA) and nitrate content in the leaves of two sorghum genotypes, one nodulating, and ‘Non-nod’ groundnut genotypes were also compared. The concentration of nitrate was lower in sorghum than in groundnut leaves, but NRA was higher in sorghum. It is suggested that either NRA in the groundnut leaves has relatively lower affinity for the substrate (higher Km, the Michaelis-Menton constant) or higher nitrate is required for the induction of nitrate reductase in groundnut than in sorghum. This implies that groundnut is a poor utilizer of fertilizer nitrogen.     

The effect of various dynamics of N availability on winter pea–wheat intercrops: Crop growth,N partitioning and symbiotic N2 fixation

Cereal–legume intercrops are a promising way to combine high productivity and several ecological benefits in temperate agro-ecosystems. However, the proportion of each species in the mixture at harvest is highly variable. The aim of this study was to test whether the timing of small application of N fertilizer is an effective way of influencing the dynamic interactions between species during crop growth and affecting the percentage of each species in the biomass of the mixture without greatly disturbing N₂ fixation. The influence of timing of nitrogen fertilization in pea–wheat intercrops was studied as regards (i) the dynamics of crop growth, (ii) nitrogen acquisition of each species, (iii) the inhibition and recovery of symbiotic N₂ fixation (SNF) after N application and (iv) final performance (yield, % of wheat, grain protein content). This was assessed in winter pea–wheat (Pisum sativum L.–Triticum aestivum L.) intercrops in 2007 and 2008 at two locations in France. Whatever the stage of application, N fertilizer tended to increase wheat growth and to decrease pea growth. N fertilization (applied once at different dates from tillering to the end of stem elongation) delayed the decrease in the contribution of wheat to total biomass and maintained the competitive ability of wheat over pea for longer than in unfertilized intercrops. N acquisition dynamics and N sharing between the two species were modified by N fertilization and its timing. Crop conditions at the time of N application (growth and phenology of each species, and their proportions in the intercrop biomass) greatly influenced intercrop response to N fertilization. Partitioning between species of soil and fertilizer N was correlated with the proportion of wheat in the total intercrop biomass observed at the date of N application. Short-term inhibition of nitrates on SNF was shown during the few days after N application, whatever its date. SNF recovery after N applications was observed only until pea flowering, but was prematurely stopped by N fertilization after this stage. The effect of N fertilization on the amount of fixed N₂ at harvest was correlated with pea biomass. N fertilization affects N₂ fixation mainly by affecting crop growth rather than %Ndfa in pea–wheat intercrops. In conclusion, N fertilization could be used as a tool to enhance the contribution of wheat in the intercrop biomass but may reduce the amount of fixed N₂ in the intercrop by decreasing pea biomass.     

氮磷钾肥对红壤区水稻增产效应的影响

通过红壤区水稻试验,分析了长期使用氮磷钾肥条件下双季水稻的生物量（包括稻谷产量和秸秆量）、养分增产效应和土壤肥力的变化。氮磷钾肥的配合施用对双季水稻生物量的影响顺序为NPK>NP>PK>NK>CK。N、P、K对水稻的增产效应系数分别为41.2%~54.8%、66.3%~88.2%和10.3%~10.9%。N、P和K增产效应的变异系数规律与其增产效应相反,说明养分对双季水稻增产效应越高,其稳定性越好。双季水稻的增产效应及其稳定性均表现为早稻优于晚稻。氮磷钾肥的配施可显著提高土壤有机质和全氮含量。磷肥的使用明显增加了土壤全磷和有效磷含量。钾肥使用提高了土壤全钾和交换性钾含量,然而水稻生物量的输出却与之相反,两者的平衡决定着土壤全钾和交换性钾含量的变化。氮磷钾肥对土壤pH值影响则不一致,氮肥（尿素）的长期使用降低土壤pH值,而磷肥（钙镁磷肥）的使用减缓了土壤pH值的降低。     

Screening of exotic potato germplasm for nitrogen uptake and biomass production

Exotic potato germplasm may contain useful traits for improving nitrogen (N) use efficiency in cultivated potatoes (Solanum tuberosum L.). The objective of this study was to evaluate a “mini” core collection of wild germplasm for biomass production, N uptake, and N use efficiency. A field study was conducted during the 1993 growing season on a loamy sand soil at Becker, Minnesota. Uniform transplants for 39 wild accessions and 3 varieties were grown under greenhouse conditions, and after 40 days, they were transplanted to the field. Plants were subjected to two N treatments, 0 and 225 kg N ha¹, replicated three times in a split-plot design. Plant parts were collected separately 111 days after transplanting, dried, weighed, and analyzed for N concentration. Nitrogen rate and potato species had significant effects on total dry weight, N content, and tissue N concentration. The regression coefficient of N concentration on total dry weight was very low (r=0.22, NS), whereas the regression coefficient of total N content on total dry weight was high (r=0.94, P>0.001). At both low and high N environments, Russet Burbank had greater dry weight than all the accessions. Several wild accessions, especiallyS. chacoense, S. commersonii, S. kurtzianum, S. microdontum, andS.phureja, had equal to or greater dry weights than Russet Norkotah or Red Norland. For N uptake efficiency, 2,7, and 20 accessions were ranked better than Russet Burbank, Russet Norkotah, and Red Norland, respectively. Recovery of soil applied N by the varieties ranged from 16 to 36%; the top seven wild accessions recovered between 27 and 49%. Based on plant growth without added N fertilizer and relative response to N fertilizer, the species were categorized into four relative N use efficiency groups: good foragers with good response to N, poor foragers with good response to N, good foragers with poor response to N, and poor foragers with poor response to N.     

Elevated atmospheric CO2 effects on N fertilization in grain sorghum and soybean

Increasing atmospheric CO₂ concentration has led to concerns about global changes to the environment. One area of global change that has not been fully addressed is the effect of elevated atmospheric CO₂ on agriculture production inputs. Elevated CO₂ concentration alterations of plant growth and C:N ratios may modify C and N cycling in soil and N fertility. This study was conducted to examine the effects of legume, soybean (Glycine max (L.) Merr.), and non-legume, grain sorghum (Sorghum bicolor (L.) Moench.) carbon dioxide-enriched agro-ecosystems on N soil fertility in a Blanton loamy sand (loamy siliceous, thermic, Grossarenic Paleudults). The study was a split-plot design replicated three times with crop species (soybean and grain sorghum) as the main plots and CO₂ concentration (ambient and twice ambient) as subplots using open top field chambers. Fertilizer application was made with ${}^{15}\text{N}$-depleted NH₄NO₃ to act as a fertilizer tracer. Elevated CO₂ increased total biomass production in all 3 years of both grain sorghum (average 30%) and soybean (average 40%). With soybean, while no impact on the plant C:N ratio was observed, the total N content was greatly increased (average 29%) due to increased atmospheric N₂ fixation with elevated CO₂ concentration. With grain sorghum, the total N uptake was not affected, but the C:N ratio was markedly increased (average 31%) by elevated CO₂. No impact of elevated CO₂ level was observed for fertilizer N in grain sorghum. The results from this study indicated that while elevated CO₂ may enhance crop production and change N status in plant tissue, changes to soil N fertilizer application practices may not be needed.     

The competitive ability of pea-barley intercrops against weeds and the interactions with crop productivity and soil N availability

Grain legumes, such as peas (Pisum sativum L.), are known to be weak competitors against weeds when grown as the sole crop. In this study, the weed-suppression effect of pea-barley (Hordeum vulgare L.) intercropping compared to the respective sole crops was examined in organic field experiments across Western Europe (i.e., Denmark, the United Kingdom, France, Germany and Italy). Spring pea (P) and barley (B) were sown either as the sole crop, at the recommended plant density (P100 and B100, respectively), or in replacement (P50B50) or additive (P100B50) intercropping designs for three seasons (2003-2005). The weed biomass was three times higher under the pea sole crops than under both the intercrops and barley sole crops at maturity. The inclusion of joint experiments in several countries and various growing conditions showed that intercrops maintain a highly asymmetric competition over weeds, regardless of the particular weed infestation (species and productivity), the crop biomass or the soil nitrogen availability. The intercropping weed suppression was highly resilient, whereas the weed suppression in pea sole crops was lower and more variable. The pea-barley intercrops exhibited high levels of weed suppression, even with a low percentage of barley in the total biomass. Despite a reduced leaf area in the case of a low soil N availability, the barley sole crops and intercrops displayed high weed suppression, probably because of their strong competitive capability to absorb soil N. Higher soil N availabilities entailed increased leaf areas and competitive ability for light, which contributed to the overall competitive ability against weeds for all of the treatments. The contribution of the weeds in the total dry matter and soil N acquisition was higher in the pea sole crop than in the other treatments, in spite of the higher leaf areas in the pea crops.     

Effects of long-term tillage,crop rotation and nitrogen fertilization on bread-making quality of hard red spring wheat

The effect of tillage system, crop rotation and nitrogen (N) fertilization rates on the quality of hard red spring wheat (Triticum aestivum L.) was studied over a 6-year period under rainfed Mediterranean conditions. Grain yield, test weight, protein content and alveogram parameters (W: alveogram index; P: dough tenacity; L: dough extensibility; P/L: tenacity–extensibility ratio; G: swelling index) were analyzed. Tillage treatments included no tillage (NT) and conventional tillage (CT). Crop rotations were wheat–sunflower (Helianthus annuus L.) (WS), wheat–chickpea (Cicer arietinum L.) (WCP), wheat–faba bean (Vicia faba L.) (WFB), wheat–fallow (WF) and continuous wheat (CW). Nitrogen fertilizer rates were 50, 100 and 150 kg N ha⁻¹ on a Vertisol (Typic Haploxerert). A split–split plot design with four replications was used. Weather conditions over the study years strongly influenced wheat yield and quality. Test weights rose considerably with yield and increased rainfall during the filling period, and fell slightly as N rates increased. Grain protein content increased with rainfall in the month of May (when grain protein accumulation occurs) up to a maximum of 80 mm. Grain protein content peaked at average mean temperatures of around 26–27°C. Protein content and alveogram parameter also improved under CT, following a prior legume crop and with rising N fertilizer rates. Alveogram parameters rose with protein content, although the P/L ratio showed greater imbalance. N fertilizer proved to be a key factor in determining bread-making quality, and the best strategy available to the farmer for optimizing wheat quality. However, the influence of weather conditions and soil residual N should be borne in mind when deciding on the additional fertilizer N to be used as a top dressing with a view to increasing yield and, particularly, enhancing wheat protein content and bread-making quality.     

Orchardgrass response to different types,rates and application patterns of dairy manure

Manure management is a difficult task on many intensive dairy farms. Crops that can utilize large quantities of manure N, yield quality forage with larger rates of manure application, and allow manure spreading at different times in a year can simplify that task. A study was conducted in 1990 and 1991 on a Copake sandy loam soil (mixed mesic) in New Milford, Connecticut. The objectives were: (1) to measure and compare dry matter (DM) response of orchardgrass (Dactylis glomerata L.) to different amounts and application times of N fertilizer and liquid and solid cattle manure; and (2) to determine crop uptake of fertilizer and manure N. Fertilizer and liquid and solid manure were applied to the soil surface annually in amounts of 150, 300 or 450 kg N ha⁻¹ in one, two or four equal applications. Orchardgrass dry matter production increased over the entire range of N amounts from all sources. Yields varied from approximately 2500 kg DM ha⁻¹ for control plots (0 kg N) to 10600 kg for plots receiving 450 kg N ha⁻¹ either as fertilizer or liquid manure. Crop response to liquid manure application was greater in year one with abundant rainfall than in year two with dry conditions during most of the growing season, whereas crop response to solid manure application improved in the second year, due to the availability of residual organic N. Orchardgrass was more sensitive to the timing of fertilizer N application than to manure N application. Despite the large differences in weather patterns experienced during this study, analysis of application patterns indicated that manure could be applied throughout the growing season to crop stubble (post-harvest) with comparable rates of uptake overall. N uptake in control plots averaged 56 kg N ha⁻¹ for both years, compared to 340 kg N ha ⁻¹ for fertilizer plots, 250 kg N ha ⁻¹ for liquid manure plots and 190 kg N ha⁻¹ for solid manure plots receiving 450 kg total N ha ⁻¹.     

Possibility of Introducing Winter Legumes,Hairy Vetch and Faba Bean,as Green Manures to Turmeric Cropping in Temperate Region

Abstract

A field experiment was conducted to examine the possibility of introducing winter legumes, hairy vetch and faba bean, as green manures to turmeric cropping in a temperate region. Hairy vetch shoots were incorporated to determine the effect of N and P added as green manure. Higher values in plant height and number of leaves of turmeric were observed in the treatment with incorporation of hairy vetch than in that without incorporation (no-incorporation) throughout the growth periods. The differences in total amounts of N and P of turmeric between incorporation and no-incorporation treatments were the highest on 15 October, when the amount was increased by 8.0 g N and 1.1 g P m^–2 compared with the no-incorporation treatment. From September to October, curcumin content rapidly increased with rhizome thickening, and gradually increased. We also quantified the N and P contribution from faba bean residues to the succeeding turmeric. The total amounts of N and P in turmeric cultivated after incorporating shoot and root residues into previously cultivated faba bean field were 2.5 g N and 1.0 g P m^–2, respectively, larger than incorporating only roots. In previously fallow field, the incorporation of the shoot increased the total amount of N and P in tumeric by 4.5 g and 1.9 g m^–2, respectively, compared with that without incorporation. In the second year after incorporation, growth and nutrient uptake of the turmeric crop did not significantly differ from those without incorporation. In the temperate region, these winter legumes would be used as basal organic matters for turmeric production.     

Improving nitrogen and phosphorus use efficiencies through inclusion of forage cowpea in the rice–wheat systems in the Indo-Gangetic Plains of India

In high productivity zones of Indo-Gangetic Plains in south Asia, the rice–wheat system is stressed due to production fatigue as evidenced by declining soil organic matter content, low efficiency of fertilizer use and diminishing rates of factor productivity. We, therefore, conducted field experiments at Modipuram, India, to conserve soil organic carbon, improve N and P use efficiency, and increase yields of rice–wheat system through inclusion of forage cowpea during the summer before cultivating the rice–wheat system. Cowpea forage harvested at 50 days removed greater amounts of N and P through aboveground biomass than those recycled through belowground roots and nodules. The NO₃-N in soil profile below 45 cm depth after wheat harvest was greater under fallow during summer than under cowpea, suggesting that cowpea minimized NO₃-N leaching beyond 45 cm depth. Similarly, in the treatments receiving both 120 kg N and 26 kg P ha⁻¹, NO₃-N in soil below 45 cm depth was lower compared to those receiving N or P alone. After three crop cycles, soil OC content in 0–15 and 15–30 cm depths was greater compared to initial OC in plots having cowpea. P applied at 26 kg ha⁻¹ increased available P content over initial P content, and also over P content of soil under no P treatments. The available P content was, however, invariably low under summer cowpea plots as compared to that under no cowpea ones. With continuous rice–wheat cropping, the bulk density (BD) of soil increased over the initial BD at different profile-depths, more so at 30–45 cm depth in no cowpea plots, but inclusion of summer cowpea helped decreasing the BD in the surface (0–15 cm) and sub-surface (15–30 and 30–45 cm) soil layers. Summer cowpea grown on residual fertility after wheat harvest did not influence rice yield, but increased wheat grain yield (P<0.05 during the terminal year), when both the crops received fertilizer N and P at recommended rates. Skipping of N or P or both, however, resulted in consistently low yield of these crops under summer cowpea treatments than those under no cowpea treatments, although the differences were not necessarily significant every year. The use efficiency of applied N and P fertilizers in rice and wheat, measured as agronomic efficiency and apparent recovery, was increased with the use of fertilizer N and P at recommended rates, and also with inclusion of summer cowpea.     

A comprehensive study of plant density consequences on nitrogen uptake dynamics of maize plants from vegetative to reproductive stages

Nitrogen (N) use efficiency (NUE), defined as grain produced per unit of fertilizer N applied, is difficult to predict for specific maize (Zea mays L.) genotypes and environments because of possible significant interactions between different management practices (e.g., plant density and N fertilization rate or timing). The main research objective of this study was to utilize a quantitative framework to better understand the physiological mechanisms that govern N dynamics in maize plants at varying plant densities and N rates. Paired near-isogenic hybrids [i.e., with/without transgenic corn rootworm (Diabrotica sp.) resistance] were grown at two locations to investigate the individual and interacting effects of plant density (low—54,000; medium—79,000; and high—104,000 pl ha⁻¹) and sidedress N fertilization rate (low—0; medium—165; and high—330 kg N ha⁻¹) on maize NUE and associated physiological responses. Total aboveground biomass (per unit area basis) was fractionated and both dry matter and N uptake were measured at four developmental stages (V14, R1, R3 and R6). Both plant density and N rate affected growth parameters and grain yield in this study, but hybrid effects were negligible. As expected, total aboveground biomass and N content were highly correlated at the V14 stage. However, biomass gain was not the only factor driving vegetative N uptake, for although N-fertilized maize exhibited higher shoot N concentrations than N-unfertilized maize, the former and latter had similar total aboveground biomass at V14. At the R1 stage, both plant density and N rate strongly impacted the ratio of total aboveground N content to green leaf area index (LAI), with the ratio declining with increases in plant density and decreases in N rate. Higher plant densities substantially increased pre-silking N uptake, but had relatively minor impact on post-silking N uptake for hybrids at both locations. Treatment differences for grain yield were more strongly associated with differences in R6 total biomass than in harvest index (HI) (for which values never exceeded 0.54). Total aboveground biomass accumulated between R1 and R6 rose with increasing plant density and N rate, a phenomenon that was positively associated with greater crop growth rate (CGR) and nitrogen uptake rate (NUR) during the critical period bracketing silking. Average NUE was similar at both locations. Higher plant densities increased NUE for both medium and high N rates, but only when plant density positively influenced both the N recovery efficiency (NRE) and N internal efficiency (NIE) of maize plants. Thus plant density-driven increases in N uptake by shoot and/or ear components were not enough, by themselves, to increase NUE.