A new sesquiterpene from the fruits of Allophylus laevigatus

From the fruits of Allophylus laevigatus a new sesquiterpene, 11-acetoxy-4 alpha-methoxyeudesmane, was isolated alongwith the known compounds carissone and apigenin-8-C-beta-rhamnopyranoside. The flavone showed no antioxidant activity in the autoxidation of beta-carotene assay.     

O/W型山苍子精油微乳的制备及其性能研究

通过加水法制备山苍子精油微乳,单因素试验考察了不同的表面活性剂、助表面活性剂、表面活性剂与助表面活性剂的质量比(K_m)以及制备温度对山苍子精油微乳形成的影响,再通过正交试验,得到水包油(O/W)型山苍子精油微乳的最佳制备条件为:曲拉通X-100(Triton X-100)为表面活性剂,无水乙醇为助表面活性剂,K_m值为1,制备温度为25℃。以最佳制备条件中混合表面活性剂与山苍子精油的质量比为9∶1,含水率为70%制得标样1,微乳的平均粒径、多分散系数(PDI)、电导率和pH值分别为13.43 nm、 0.125、 83 mS/m和5.82。对标样1进行性能测试分析,发现其在1 000～4 000 r/min的速率下进行离心时,微乳外观无变化,可证明其离心稳定性较好;在标样1和精油中加入等量的亚甲基蓝水溶液,亚甲基蓝水溶液在标样1中的扩散速度显著快于在精油中的,可证明微乳的水溶性显著优于精油;在缓释12 h后,微乳中缓释的柠檬醛含量显著少于精油中缓释的,说明微乳的缓释性优于精油;在10～30 g/L的质量浓度范围内,山苍子精油微乳液标样1比精油表...     

Screening antiacne potency of Indonesian medicinal plants: antibacterial,lipase inhibition,and antioxidant activities

Indonesian medicinal plants were screened as potential sources of antiacne agents. The screening methods were performed using antibacterial assay against Propionibacterium acnes, lipase inhibitor assay, and antioxidant assay. The results showed that from 40 plant materials extracted with methanol and 50% ethanol in water, Caesalpinia sappan was the best extract based on the combined activities: antibacterial (minimum inhibitory concentration 0.13 mg/ml; minimum bactericidal concentration 0.25 mg/ml), lipase inhibitory [50% inhibitory concentration (IC₅₀) 120.0 μg/ml], and antioxidative (IC₅₀ 6.47 μg/ml). Another prospective extract is Intsia palembanica based on its lipase inhibitory activity (IC₅₀ 4.1 μg/ml) and antioxidant activity (IC₅₀ 3.87 μg/ml). Part of this report was presented at the 58th Annual Meeting of the Japan Wood Research Society, Tsukuba, March 2008     

Anti-inflammatory, antioxidant and antifungal furanosesquiterpenoids isolated from Commiphora erythraea (Ehrenb.) Engl. resin

The topical anti-inflammatory, free radical scavenging and antifungal activities of essential oils and extracts of Commiphora erythraea (Ehrenb.) Engl. resin were investigated. The hexane extract significantly inhibited oedema when applied topically in Croton oil-induced ear oedema assay in mice. The same extract showed antioxidant activity in DPPH radical scavenging assay. A bioguided separation of the hexane extract led to the isolation of furanosesquiterpenoids 1 and 2 that showed a weak antifungal activity, while compounds 3-5 resulted to be antioxidant (EC₅₀ 4.28, 2.56 and 1.08 mg/mL, respectively) and anti-inflammatory (30, 26 and 32% oedema reduction, respectively).     

Preliminary antinociceptive, antioxidant and cytotoxic activities of Leucas aspera root

The ethanolic extract of Leucas aspera root was subjected to acetic acid induced writhing inhibition, 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay and brine shrimp lethality bioassay for screening of antinociceptive, antioxidant and cytotoxic activity, respectively. The extract produced significant writhing inhibition in acetic acid induced writhing in mice at the doses of 250 and 500 mg/kg. The extract showed a significant free radical scavenging activity with an IC(50) of 8 microg/ml. The extract showed significant lethality to brine shrimp with an LC(50) value.     

Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells

Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.     

山苍子果实亚临界低温制油的研究

山苍子是我国特有的香料植物,资源量主要分布于我国湖南、广西和贵州等省份,资源量丰富,但是目前利用率仅为5%左右,其余组分主要以丢弃为主,造成资源的极大浪费。本研究创新引入亚临界工艺进行山苍子核仁油的制取,得出最佳操作因素组合为含水率6%,温度50℃,萃取时间45 min,最佳工艺条件下萃取率大于95%。通过对山苍子核仁油的脂肪酸分析,可知山苍子核仁油主要成分为月桂酸,占53.3%。可见月桂酸是用途广泛的一种工业原料,经济价值较高,因此提取山苍子核仁油具有很好的经济效益和市场前景,本研究可为亚临界工艺在山苍子制油领域的推广应用提供坚实的理论和技术支撑。     

Antimutagenic, antioxidant and antimicrobial properties of Maytenus krukovii bark

The hydroalcoholic extract of Maytenus krukovii bark was investigated for its in vitro mutageno-protective activities by means of the Ames Salmonella/microsome assay. The extract showed an inhibitory effect in both T98 and T100 strains against the mutagenic activity of promutagen 2-aminoanthracene but was not protective against directly acting mutagens sodium azide and 2-nitrofluorene. When tested as a radical scavenger and antioxidant it produced a dose-dependent inhibition. The extract did not show significant antibacterial properties, and was weakly active against dermatophyte and phytopathogenic fungi, but inhibited the growth of phytopathogen Pithyum ultimum.     

山苍子光合速率、蒸腾速率的研究

在晴天适宜条件下,使用LI-6400 p便携式光合测定系统测定不同年龄阶段的山苍子(Litsea cubeba)连体叶片的光合速率和蒸腾速率。结果表明:山苍子的光合作用日变化均呈双峰型,存在光合午休现象,并且3 a生山苍子的光合速率高于2 a生的,1 a生的最低。且蒸腾速率与光合速率间存在相同的变化趋势。     

Free radical scavenging activity of three Equisetum species from Fruska gora mountain

The scavenger activities of Equisetum arvense, Equisetum ramosissimum and Equisetum telmateia aboveground parts phosphate buffer (pH 7) extracts were evaluated using three different methods: DPPH assay, ESR and NO radical inhibition assay. Total reducing power was determined by FRAP assay. The E. telmateia extract demonstrated the most relevant scavenger and antioxidant properties. ESR signal of DMPO-OH radical adducts in the presence of E. telmateia phosphate buffer (pH 7) extract was reduced to 98.9% which indicated that E. telmateia could be a useful source of antioxidants with huge scavenger ability.     

Antioxidant activity of Filipendula hexapetala flowers

The antioxidant activity of the methanol extract of Filipendula hexapetala flowers was assessed by the assay for ferric-reducing antioxidant power (FRAP), the assay for DPPH free radical scavenging ability (DPPH) and the assay for the influence of lipid peroxidation in liposomes, induced by Fe(2+)/ascorbate system and measured by the TBA test (LP). The activity of the investigated extract in all test-systems was found to be significant. The principal constituent responsible for the observed effects was isolated and identified as spiraeoside.     

山苍子油的深加工及产品利用

山苍子是中国特色芳香油植物资源之一。山苍子油的用途十分广泛,特别是其主要成份柠檬醛的深加工产品。本文介绍了山苍子油主要成份柠檬醛的深加工产品的合成方法及产品的用途,主要是在香料行业和药物中间体方面的应用。     

Antioxidant and antibacterial activities of aqueous extract of Seabuckthorn (Hippophae rhamnoides) seeds

Seabuckthorn (Hippophae rhamnoides) seeds aqueous extract was screened for antioxidant and antibacterial activities. The antioxidant activities (reducing power, DPPH and liposome model system) showed a good antioxidant activity. The extract was also found to possess antibacterial activity with a MIC values with respect to Listeria monocytogenes and Yersinia enterocolitica found to be 750 and 1000 ppm, respectively. The antioxidant and antimicrobial effects of the extract implicate its potential for natural preservation.     

Antioxidant activity of Arbutus unedo leaves

The ethanol and methanol extracts of Arbutus unedo leaves were screened for antioxidant activity. The antioxidant activity was determined by an improved assay based on the decolorization of the radical monocation of [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS). The ethanol and methanol extract of A. unedo leaves displayed potent antioxidant activity.     

Preliminary assay on the radical scavenging activity of olive wood extracts

The dichloromethane and ethanol extracts of Olea europaea wood (picual olive cultivar) were screened for antioxidant activity, determined by the DPPH free radical scavenging assay. The ethanol extract displayed potent antioxidant activity.     

Antioxidant activity of Cassia auriculata flowers

The ethanol and methanol extracts of Cassia auriculata flowers were screened for antioxidant activity. The antioxidant activity was determined by an improved assay based on the decolorization of the radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The ethanol and methanol extracts of C. auriculata flowers showed antioxidant activity in both assays.     

我国山苍子的研究现状及展望

综述了山苍子的生物学特征、用途,以及近年来山苍子的繁殖技术、造林技术、精油的提取纯化等方面的研究进展;总结了山苍子资源开发利用中存在的问题,提出山苍子研究的方向。     

福建省山苍籽成熟期的数理统计研究

山苍籽是野生香料植物，广泛分布于我国长江以南各省。山苍籽经水蒸气蒸馏可获得山苍籽精油。是我国一种重要的天然香料产品，其主要成分为柠檬醛。本文对山苍籽成熟过程中的精油得率，含醛量，水分含量变化规律进行了研究鼗所得的有关数据进行数理统计分析，用数学方程式表达出山苍籽成熟过程中的各项指标的变化规律。     

江西山苍子开发利用现状及发展趋势

介绍江西省山苍子的自然分布、人工栽植概况及其开发利用现状,讨论江西山苍子在开发利用及产业化发展中存在的问题,提出解决办法及努力方向。     

Bioactive polar compounds from stem bark of Magnolia officinalis

Two new phenylethanoid glycosides magnoloside D (1) and E (2), together with nine known compounds, were isolated from the polar part of methanol extract of the stem bark of Magnolia officinalis. The structures of the new compounds were established on the basis of spectral analysis. Anti-spasmodic activity of four major constituents (3, 4, 9 and 11) was tested in isolated colon of rat, compounds 3, 4, and 9 showed inhibition against acetylcholine, with the effect similar to that of magnolol and honokiol. At the same time, antioxidant activity of the isolated compounds was investigated using a DPPH and an ORAC assay. All of the compounds, except compound 8 showed potent antioxidant capacity in the ORAC assay, while compounds 1-5 and 11 exhibited potent antioxidant activity in the DPPH assay.