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1.
Patrícia Aparecida Cardoso da LUZ Paulo Ramos da Silva SANTOS Cristiana ANDRIGHETTO André Mendes JORGE Ant?nio Chaves de ASSIS NETO 《The Journal of reproduction and development》2013,59(1):14-17
Buffalo are an economically important source for meat and milk production, especially in
Brazil. However, important aspects of their biology remain unknown thus far. Herein, we
describe the reproductive characteristics of male Murrah buffalo (Bubalus
bubalis) raised under extensive management conditions by applying biometrics
associated with testicular weight. We analyzed seven males, divided into two groups: G1,
which consisted of four 18-month-old animals, and G2, which consisted of three
24-month-old animals. Testicular development occurs slowly in Murrah buffalo, suggesting a
delay of sexual maturity. The biometric testicular parameters analyzed were scrotal
circumference, testicular weight, testicular length, testicular width, testicular
thickness and testicular circumference. Our data indicate strong correlations between SC,
age and body weight, and additional significant relationships were identified between body
weight, age and other testicular parameters. Thus, these parameters are suitable
indicators when selecting bulls for breeding purposes. 相似文献
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Miyuki KITAOKA Shuichi HIRAI Hayato TERAYAMA Munekazu NAITO Ning QU Naoyuki HATAYAMA Hidenobu MIYASO Yoshiharu MATSUNO Masatoshi KOMIYAMA Masahiro ITOH Chisato MORI 《The Journal of reproduction and development》2013,59(5):485-490
Exposure to di-(2-ethylhexyl) phthalate (DEHP) has been reported to induce
spermatogenic disturbance through oxidant stress and affect the immune system as an
adjuvant. However, the effect of DEHP on the testicular immune microenvironment has
not yet been investigated. In the present study, we examined the testicular immune
microenvironment after exposure to doses of DEHP, previously identified as
no-observed-adverse-effect levels. Adult male mice were administered food containing
0%, 0.01% or 0.1% DEHP and then testes were analyzed. The results showed that a
slight but significant spermatogenic disturbance appeared in the 0.1% DEHP group but
not in the 0.01% DEHP group at 8 weeks. It was also demonstrated that lymphocytes and
F4/80- and MHC class II- positive cells were significantly increased with the
elevation of IL-10 and IFN-γ mRNA expressions in the testes of not only the 0.1% DEHP
group but also the 0.01% DEHP group at 8 weeks. Histochemical analyses involving
horseradish peroxidase (HRP) as a tracer showed that a little blood-borne HRP had
infiltrated into the lumen of a few seminiferous tubules beyond the
blood-testis-barrier in both the 0.1% and 0.01% DEHP groups at 8 weeks. This
indicates that a dose of DEHP that has little effects on spermatogenesis can change
the testicular immune microenvironment with functional damage of the blood-testis
barrier. 相似文献
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Samy M ELEAWA Mahmoud A ALKHATEEB Fahaid H ALHASHEM Ismaeel BIN-JALIAH Hussein F SAKR Hesham M ELREFAEY Abbas O ELKARIB Riyad M ALESSA Mohammad A HAIDARA Abdullah S. SHATOOR Mohammad A KHALIL 《The Journal of reproduction and development》2014,60(2):115-127
This study was performed to investigate the protective and therapeutic effects of
resveratrol (RES) against CdCl2-induced toxicity in rat testes. Seven
experimental groups of adult male rats were formulated as follows: A) controls+NS, B)
control+vehicle (saline solution of hydroxypropyl cyclodextrin), C) RES treated, D)
CdCl2+NS, E) CdCl2+vehicle, F) RES followed by CdCl2
and M) CdCl2 followed by RES. At the end of the protocol, serum levels of FSH,
LH and testosterone were measured in all groups, and testicular levels of TBARS and
superoxide dismutase (SOD) activity were measured. Epididymal semen analysis was
performed, and testicular expression of Bcl-2, p53 and Bax was assessed by RT-PCR. Also,
histopathological changes of the testes were examined microscopically. Administration of
RES before or after cadmium chloride in rats improved semen parameters including count,
motility, daily sperm production and morphology, increased serum concentrations of
gonadotropins and testosterone, decreased testicular lipid peroxidation and increased SOD
activity. RES not only attenuated cadmium chloride-induced testicular histopathology but
was also able to protect against the onset of cadmium chloride testicular toxicity.
Cadmium chloride downregulated the anti-apoptotic gene Bcl2 and upregulated the expression
of pro-apoptotic genes p53 and Bax. Resveratrol protected against and partially reversed
cadmium chloride testicular toxicity via upregulation of Bcl2 and downregulation of p53
and Bax gene expression. The antioxidant activity of RES protects against cadmium chloride
testicular toxicity and partially reverses its effect via upregulation of BCl2 and
downregulation of p53 and Bax expression. 相似文献
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The objective of this study was to investigate the arterial patterns of the stallion testis in relation to testicular shape. Two hundred and fifty-one stallion testes were evaluated for the presence of the lateral branches of the testicular artery. Seven specimens had their testicular arteries filled with latex milk, fixed in 70% alcohol, and dissected. Two hundred six specimens (82%) had a single testicular artery and no lateral branches; 39 testes (16%) had one lateral branch of the testicular artery; and six testes (2%) had two lateral branches of the testicular artery each. The lateral branches of the testicular artery obtained from the adult stallions, more than 5 years old, were associated with distinct lateral bulging, giving them a pear-like shape, whereas similar vascular pattern in young colts, less than 1 year old, did not cause similar shape change. Five distinct patterns of the branching of the testicular artery were determined. We concluded that the lateral branches of the testicular artery are present in approximately 20% of stallion testes. This anatomic pattern is associated with a lateral bulge that develops slowly over several years and is associated with a change in testicular shape from an ellipsoid in colts to a pear-like shape in adult stallions. 相似文献
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分别应用复合黏膜免疫佐剂Ⅰ和Ⅱ与新城疫Ⅳ系弱毒苗混合(佐剂Ⅰ组和佐剂Ⅱ组),经口免疫鸡后,研究鸡小肠黏膜局部CD3^+T细胞、上皮内淋巴细胞和干扰素-γ(Interferon-γ,IFN-γ)mRNA表达水平的变化。结果表明,在整个免疫期内CD3^+T细胞和上皮内淋巴细胞数量都呈上升趋势,在首免后第3和第5周时十二指肠CD3^+T细胞佐剂Ⅱ组比佐剂Ⅰ组显著增加(P〈0.05),空肠CD3^+T细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加(P〈0.01),第7周时Peyer’s斑上皮内淋巴细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加(P〈0.01);整个免疫期内佐剂Ⅱ组和佐剂Ⅰ组IFN-γmRNA表达水平呈下降趋势,表达量较低。本研究表明2种复合黏膜免疫佐剂都能明显增加黏膜局部免疫细胞的数量,提高小肠局部黏膜免疫力。 相似文献
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为了探讨不同佐剂的奶牛乳房炎多联苗对小鼠免疫机能的影响,笔者将小白鼠分为3个组,每组30只,分别为蜂胶苗组、铝胶苗组和空白对照组。用制备的氢氧化铝胶和蜂胶2种乳房炎佐剂多联苗分别免疫小鼠,分别于免疫后的7、14、21、30和45 d采血,进行2种多联苗免疫后E-玫瑰花环率和淋巴细胞转化率测定。结果表明,蜂胶和铝胶2种佐剂苗与空白对照组相比均能显著提高小鼠的细胞免疫水平,2种苗的细胞免疫水平均在免疫后30 d左右达到最高峰(P<0.01),但在免疫后第30和45天时E-玫瑰花环率和淋巴细胞转化率,蜂胶苗组均极显著高于铝胶苗组(P<0.01),说明蜂胶佐剂比铝胶佐剂能更强地增强多联苗抗原对小鼠T细胞的致敏程度。该研究为进一步明确乳房炎多联苗免疫机制及筛选更好的免疫佐剂提供了科学数据。 相似文献
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为研究比较5种塞内卡病毒疫苗佐剂,分别用4种自主研发佐剂和进口ISA206佐剂配制塞内卡病毒灭活疫苗,检测疫苗的理化性质、安全性及免疫效力,并对检测结果进行对比分析。结果显示,进口ISA206佐剂疫苗黏度为43.02 cP,自主研发佐剂疫苗黏度均在27.88 cP以下,其他理化性质无差异;5批疫苗安全性试验均未见明显异常;5批疫苗免疫动物后均可诱导机体产生中和抗体,二免后14 d抗体滴度在211以上,4批自主研发佐剂疫苗免疫组抗体滴度水平高于进口ISA206佐剂疫苗约1个滴度,攻毒保护结果均在4/5以上,2批自主研发佐剂疫苗免疫组保护率可达5/5。结果表明,用4种自主研发佐剂制备的塞内卡病毒灭活疫苗质量不低于用进口ISA206佐剂制备的塞内卡病毒灭活疫苗。 相似文献
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Masataka CHIHARA Saori OTSUKA Osamu ICHII Yasuhiro KON 《The Journal of reproduction and development》2013,59(6):525-535
The blood testis-barrier (BTB) is essential for maintaining homeostasis in the
seminiferous epithelium. Although many studies have reported that vitamin A (VA) is
required for the maintenance of spermatogenesis, the relationships between the BTB,
spermatogenesis and VA have not been elucidated. In this study, we analyzed BTB
assembly and spermatogenesis in the testes of mice fed the VA-deficient (VAD) diet
from the prepubertal period to adulthood. During the prepubertal period, no changes
were observed in the initiation and progression of the first spermatogenic wave in
mice fed the VAD diet. However, the numbers of preleptotene/leptotene spermatocytes
derived from the second spermatogenic wave onwards were decreased, and initial BTB
formation was also delayed, as evidenced by the decreased expression of mRNAs
encoding BTB components and VA signaling molecules. From 60 days postpartum, mice fed
the VAD diet exhibited apoptosis of germ cells, arrest of meiosis, disruption of the
BTB, and dramatically decreased testis size. Furthermore, vacuolization and
calcification were observed in the seminiferous epithelium of adult mice fed the VAD
diet. Re-initiation of spermatogenesis by VA replenishment in adult mice fed the VAD
diet rescued BTB assembly after when the second spermatogenic wave initiated from the
arrested spermatogonia reached the preleptotene/leptotene spermatocytes. These
results suggested that BTB integrity was regulated by VA metabolism with meiotic
progression and that the impermeable BTB was required for persistent spermatogenesis
rather than meiotic initiation. In conclusion, consumption of the VAD diet led to
critical defects in spermatogenesis progression and altered the dynamics of BTB
assembly. 相似文献
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本试验旨在探讨日粮添加不同水平芦丁对缓解热应激小鼠睾丸组织损伤的影响。将30只5周龄体重相近(20~22 g)的ICR系雄性小鼠随机分为5组,各组小鼠分别饲喂基础日粮添加0(CON组)、0(HS组)、250(HS+R250)、500(HS+R500)和1 000(HS+R1000) mg·kg-1芦丁的日粮,饲养10 d后,除对照组(CON)外,所有小鼠在每天10:00至14:00之间置于42℃恒温箱中连续处理8 d后屠宰取样分析。结果显示:1)与CON组相比,HS组小鼠睾丸指数无显著变化(P>0.05),而日粮添加250 mg·kg-1芦丁显著提高热应激小鼠睾丸指数(P<0.05);小鼠睾丸组织切片结果显示,与CON组相比,HS组小鼠生精小管直径和横截面积显著降低(P<0.05),生精细胞脱落比率显著增加(P<0.05);与HS组相比,HS+R250组小鼠生精小管横截面积显著增加(P<0.05),生精细胞脱落比率显著降低(P<0.05),HS+R500组生精细胞脱落比率也显著降低(P<0.05),HS+R1000组小鼠生精小管直径显著增加(P<0.05);小鼠睾丸指数、生精小管直径及生精小管脱落比率在芦丁处理组与CON组之间无显著差异(P>0.05),但HS+R250组小鼠睾丸生精小管横截面积显著高于CON组(P<0.05)。2)与CON组相比,HS组小鼠睾丸组织丙二醛(MDA)含量显著升高(P<0.05),总抗氧化能力(T-AOC)与还原型谷胱甘肽(GSH)含量以及血红素酶-1(HO-1) mRNA的表达量均显著降低(P<0.05);日粮添加250 mg·kg-1芦丁显著降低热应激小鼠睾丸组织中MDA含量(P<0.05),提高T-AOC与GSH含量(P<0.05),并增强核因子E2相关因子2(Nrf2)、HO-1和谷胱甘肽过氧化物酶(GSH-Px) mRNA的表达量(P<0.05),而添加500和1 000 mg·kg-1芦丁也显著提高了GSH含量(P<0.05);与CON组相比,除HS+R250组Nrf2 mRNA表达量显著高于CON组(P<0.05)外,芦丁组热应激小鼠睾丸组织抗氧化相应基因与酶活指标均无显著性变化(P>0.05)。3)热应激小鼠睾丸中核因子-κB (NF-κB)、TOLL样受体4(TLR-4)和Bax的mRNA表达量显著增加(P<0.05),而Bcl-2表达量显著降低(P<0.05);日粮添加250 mg·kg-1芦丁显著降低NF-κB、TLR-4、髓样分化因子88(MyD88)、白细胞介素-Iβ(IL-1β)和Bax mRNA表达量(P<0.05),增强Bcl-2的表达水平(P<0.05);添加500 mg·kg-1芦丁显著降低NF-κB和TLR-4表达量(P<0.05),而1 000 mg·kg-1芦丁能够显著增加Bcl-2的表达(P<0.05);小鼠睾丸组织中免疫和增值凋亡相关基因的表达量在添加芦丁组及CON组之间无显著性差异(P>0.05)。结果提示,日粮添加适宜剂量芦丁具有改善热应激小鼠睾丸组织形态及功能的效果,其机制可能与芦丁通过Nrf2信号通路缓解氧化应激,通过TLR-4/NF-κB信号通路抑制炎症反应及调控Bax/Bcl-2 mRNA的表达密切相关。本试验条件下日粮添加250 mg·kg-1芦丁的效果较好。 相似文献
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本研究采用组织病理学和免疫组织化学技术观察了三聚氰胺(Melamine,MA)对雄性小鼠睾丸的毒性作用,以深入揭示三聚氰胺的毒性作用及其机理。将25只28日龄SPF雄性昆明小鼠随机均分为5组,1组为对照组,其他4组为试验组。4个试验组分别按下述不同剂量灌服三聚氰胺:0.6 mg/kg(bw)/d(每日每公斤体重0.6 mg)、3.0 mg/kg(bw)/d、15.0 mg/kg(bw)/d和17.5 mg/kg(bw)/d,用玉米油做溶剂;空白对照组每日灌服等量玉米油。连续灌胃30天后剖检小鼠并取其睾丸组织进行固定、切片和染色,观察睾丸的组织病理变化。同时,采用免疫组织化学方法检测睾丸组织中增殖细胞核抗原(PCNA)的表达量。观察结果表明,与对照组相比,试验组不同剂量浓度的三聚氰胺对小鼠睾丸组织造成不同程度的病理损伤;三聚氰胺也会在一定程度上影响睾丸组织中PCNA的表达。研究结果表明三聚氰胺对于小鼠生殖系统有明显毒性作用。 相似文献
16.
Futoshi YAZAMA Haruki SATO Tomoko SONODA 《The Journal of reproduction and development》2015,61(5):399-406
An experimental ischemia (EI)-induced mouse model was used to
analyze pathological and biochemical alterations in testes. Initial
morphological changes were observed in Sertoli cells of EI testes at
the light microscopic level. Examination of the ultrastructure using
transmission electron microscopy confirmed that Sertoli cells were
partially detached from the basement membrane of the seminiferous
epithelium and that the cell membranes of adjacent Sertoli cells were
not joined. The functional integrity of the blood-testis barrier (BTB)
was assessed using the lanthanum tracer technique. Lanthanum had
penetrated into the spaces between adjacent Sertoli cells in the
adluminal compartment up to the lumen of the seminiferous epithelium
in EI testes. Proteome analysis showed that the expression of heat
shock protein (HSP) 70 was significantly upregulated in EI testes.
Western blot analysis confirmed that the expression of HSP70 increased
in a time-dependent manner after the EI procedure. HSP70
immunostaining was observed in spermatocytes and in round and
elongated spermatids in EI testes. Our results suggest that a change
in the junctions between adjacent Sertoli cells on the basal
compartment is involved in the BTB disruption in EI testes. Therefore,
male infertility caused by the BTB disruption could be associated with
heat stress induced by ischemia. 相似文献
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The saponin adjuvant Quil A has been investigated in the vaccination of cattle against foot-and-mouth disease. Using a Frenkel type vaccine a dose-response relationship has been established between Quil A and neutralizing antibody titres. Ten ml of vaccine was combined with 0, 50, 200, 800, and 3200 µg of Quil A. The combinations were each injected into 4 animals. The local reaction on the site of injection produced by injection of the vaccine alone and in combination with different doses of Quil A has been estimated. On this basis a therapeutical dose at 1 mg of Quil A has been estimated to combine maximum adjuvant effect with a minimum of adverse reactions. This dose has been tested in the vaccination of cattle with FMD vaccines derived from BHK suspension cell virus of type O and A respectively. The vaccines were tested in 10 ml and 5 ml doses with or without Quil A, and each in 4 animals. It is concluded that Quil A is a valuable adjuvant for use in the induction of neutralizing antibodies against foot-and-mouth disease in cattle. 相似文献
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Casey L. Donnelly MS Christophe Staub PhD Dickson Varner PhD DVM Terry Blanchard PhD DVM Larry Johnson PhD David W. Forrest PhD 《Journal of Equine Veterinary Science》2007,27(5):212-216
Apoptosis is necessary for both initiation and control of spermatogenesis; however, an increase in apoptosis can lead to subfertility/infertility in stallions, causing substantial financial loss in the equine industry. The ability of stem cell factor (SCF), leukemia-inhibiting factor (LIF), granulocyte–macrophage colony-stimulating factor (GM-CSF), and estradiol (E2), alone or in combination, to prevent apoptosis of germ cells in short-term equine testicular cultures was examined. Testicular tissue was sectioned into approximately 2-mm cubes and placed in media-filled culture chambers. Concentrations of SCF (100 ng/mL), LIF (10 ng/mL), GM-CSF (5 ng/mL), and E2 (10−9 mol/l) were added alone or in combination to each well. After 6 hours in culture, the tissue was fixed and immunohistochemically (terminal deoxynucleotidyl transferase-mediated nick-end labeling; TUNEL) stained for apoptosis detection. Apoptotic cells per 100 Sertoli cell nuclei within seminiferous tubules were counted until the 500th Sertoli cell nuclei was reached. This counting procedure was used for each slide. An analysis of variance (ANOVA) with a Tukey's test was used to compare apoptotic rates. In comparison with the control, GM-CSF alone lowered apoptosis by 34.77%. GM-CSF–treated tissue combined with SCF and LIF as well as GM-CSF combined with SCF, LIF, and E2 reduced apoptosis when compared with the control (37.45% and 44.40%, respectively) or other treatment combinations. GM-CSF alone reduced apoptosis; results suggest possible synergy for the combinations of SCF and LIF with GM-CSF and for E2 with SCF, LIF, and GM-CSF. 相似文献
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The study was aimed to prepare vaccines with different adjuvants,and research its effects on immunogenicity.The PCV2 Cap gene with its signal peptide removed was connected to pET-28a vector,and then was induced to express,using sodium deoxycholate(DOC)and low concentration of urea to dissolve the inclusion body.Different adjuvants,such as alum-based adjuvants,liposome adjuvants,propolis adjuvants,white oil adjuvants and Freund adjuvant were prepared,together with protein purified to make up subunit vaccines,and commercial inactivated vaccine as positive control,immuning mice,and ELISA method were used to detect changes in concentrations of animal serum antibody and cytokines,evaluated the immune protective effect.Results showed that the expression product in the form of inclusion body,using the DOC could omit dissolving inclusion body protein renaturation steps,and the purification method was simple,the capsid protein obtained had high purity.ELISA assays showed that PCV2-Cap had good immunogenicity.And we found that the water system adjuvants had high immune activity,this could provide important previous experimental data for the commercialization of the subunit vaccine. 相似文献