共查询到3条相似文献,搜索用时 0 毫秒
1.
Michiko NAKAI Manabu OZAWA Naoki MAEDOMARI Junko NOGUCHI Hiroyuki KANEKO Junya ITO Akira ONISHI Naomi KASHIWAZAKI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2014,60(3):256-259
In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from
in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on
embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At
10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until
168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI
embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups
were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic
development. 相似文献
2.
Kanokwan SRIRATTANA Mariena KETUDAT-CAIRNS Takashi NAGAI Masahiro KANEDA Rangsun PARNPAI 《The Journal of reproduction and development》2014,60(5):336-341
Trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several
species. This brings our attention to investigation of the effects of TSA on developmental potential of swamp buffalo cloned
embryos. Swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp
buffalo enucleated oocytes. After fusion, reconstructed oocytes were treated with 0, 25 or 50 nM TSA for 10 h. The results
showed that there was no significant difference in the rates of fusion (82–85%), cleavage (79–84%) and development to the
8-cell stage (59–65%) among treatment groups. The highest developmental rates to the morula and blastocyst stages of embryos
were found in the 25 nM TSA-treated group (42.7 and 30.1%, respectively). We also analyzed the DNA methylation level in the
satellite I region of donor cells and in in vitro fertilized (IVF) and cloned embryos using the bisulfite
DNA sequencing method. The results indicated that the DNA methylation levels in cloned embryos were significantly higher than
those of IVF embryos but approximately similar to those of donor cells. Moreover, there was no significant difference in the
methylation level among TSA-treated and untreated cloned embryos. Thus, TSA treatments at 25 nM for 10 h could enhance the
in vitro developmental potential of swamp buffalo cloned embryos, but no beneficial effect on the DNA
methylation level was observed. 相似文献
3.
Toshinori OIKAWA Tomoko ITAHASHI Takashi NUMABE 《The Journal of reproduction and development》2016,62(1):11-16
The purpose of this study was to determine whether dithiothreitol (DTT) treatment of sperm and ethanol
activation improve embryo production by intracytoplasmic sperm injection (ICSI). Further, we compared ICSI
with standard in vitro fertilization (IVF) in oocytes obtained from cattle. We demonstrated
that DTT reduced the disulfide bond in the bovine sperm head. Using oocytes obtained from a slaughterhouse,
ICSI-DTT treatment without ethanol showed the highest rate of blastocyst formation. We applied these results
to fertilization using ovum pick-up (OPU). Eleven Japanese black cattle served as donors for OPU plus standard
IVF (OPU-IVF). Of them, four donors with low embryo development rates were selected to determine whether
embryo development was enhanced by OPU plus ICSI (OPU-ICSI). We assessed effects on embryo development
following IVF and ICSI in oocytes obtained using OPU. Blastocyst rates were significantly higher for OPU-ICSI
than for OPU-IVF. Our results suggest that OPU-ICSI improves the blastocyst development rate in donors with
low embryo production compared with the standard OPU-IVF. 相似文献