共查询到20条相似文献,搜索用时 31 毫秒
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麦冬是我国传统中药之一,兼备保健和治疗作用.为了探究麦冬能否应用在宠物食品中,本试验以不同浓度的麦冬水提物(3.91~8000μg/mL)为受试药物,从细胞的形态、增殖、凋亡及周期4个方面对猫肾细胞(F81)进行安全性评价.结果表明:在试验浓度范围内,麦冬水提物没有抑制F81的增殖,且在3.91~62.5μg/mL的浓... 相似文献
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鸡视网膜移动性无长突细胞的分布 总被引:2,自引:0,他引:2
利用Nissl染色、视神经溃变试验和计算机图像处理的方法,研究了30日龄鸡(P30)视网膜移动性无长突细胞(displace
damacrine 相似文献
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为了提高野牦牛成纤维细胞分离培养效果,研究了组织保存方法、原代培养方法、培养液类型、添加细胞生长因子和冷冻保存液配方对野牦牛体细胞分离培养与传代的作用。结果表明,PBS、D/F12和TCM199适合野牦牛皮肤组织的保存,D/F12和TCM199培养液适合组织块培养,组织块成活率达到(86.29±4.62)%,组织块法适合野牦牛成纤维细胞的原代培养;D/F12培养液培养野牦牛成纤维细胞效果较好,群体倍增时间和平台期密度分别为38.47 h和2.075×106/mL,正常二倍体核型百分率为84.33%;表皮生长因子(EGF)和碱性成纤维细胞因子(bFGF)产生了较明显的促增殖作用,平台期细胞密度明显增加;2种冷冻保存液(D/F12添加胎牛血清和二甲亚砜及胎牛血清添加二甲亚砜)均能用于野牦牛细胞冷冻,细胞存活率分别是87.33%和89.00%。 相似文献
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Primary splenic stromal tumors have rarely been reported in rodents. We report the case of a 90-week-old male WBN/Kob rat with a nodular demarcated mass in the spleen, which was kept as a non-treated animal in a long-term animal study. Histopathology revealed round to short spindle-shaped tumor cells arranged in a solid growth pattern. Invasive growth, anisokaryosis, and high mitotic activity (46 per 10 high-power fields [2.37 mm2]) were observed to be multifocal, but most tumor cells showed mild nuclear pleomorphism. The pattern of silver impregnation corresponded to that of the marginal zone of the red pulp. Immunohistochemistry revealed that the tumor cells were double positive for fascin and desmin and focally positive for Iba-1 and OX-6 expression. These characteristics were similar to those observed in fibroblastic reticular cells and dendritic cells in the marginal zone of the red pulp. These findings suggest that the malignant stromal cell tumor of the spleen in this case had characteristics of both fibroblastic reticular cells and dendritic cells. 相似文献
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北京鸭视网膜节细胞层细胞的分布 总被引:3,自引:0,他引:3
利用 Nissl染色和计算机图像处理的方法 ,研究了 3 0日龄北京鸭 (P3 0 )视网膜节细胞层 (ganglion cell layer,GCL )细胞的形态大小、数量和密度分布。结果表明 ,北京鸭视网膜节细胞层细胞形态多样 ,有圆形、椭圆形和多角形等。不同区域细胞大小差异显著 ,由视网膜中央区向周边部逐渐增大 (CA为 (53 .3 2± 2 0 .53 )μm2 ;NP 为 89.73± 53 .0 1 μm2 ;TP 为1 4 8.71± 86.2 1 μm2 )。细胞数量分布很不均匀 ,在视网膜中央有一个高密度区即中央高密度区(CA约为为 1 0 660个 / mm2 ) ,且由视网膜中央部向视网膜周边部细胞密度逐渐降低 (TP约为53 40个 / mm2 ;NP约为 583 0个 / mm2 )。可见 ,节细胞层细胞由中央区至周边部由小至大递增而细胞密度梯度呈现递减的变化 相似文献
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体细胞克隆绵羊“多莉”(Dolly)的诞生,表明成年哺乳动物体细胞具有基因组的全能性。本文从“多莉”诞生的背景、体细胞克隆技术的研究现状、体细胞核移植过程中的核质互作、该技术目前存在的问题及其在制作转基因动物上的应用等方面,概述了体细胞克隆技术的研究进展。 相似文献
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利用免疫组织化学和形态计测学的方法 ,观察了 1 8匹成熟雌性蒙古马的脑垂体前叶生长激素细胞和催乳激素细胞的数量和面积 ,同时利用放射免疫分析方法检测了这两种激素的血浆水平。结果表明 ,每个马脑垂体前叶中 ,生长激素细胞的平均数量为 6 .42× 1 8,每个细胞的平均面积为 82 .40μm2 ;催乳激素细胞的平均数量为 6 .0 7× 1 0 8,每个细胞的平均面积为 47.31μm2 。生长激素的血浆含量平均为 2 .84ng/ m L,但个体差异较大 ,变异系数高达 78.5 % ,催乳激素的血浆含量平均为 7.2 6 ng/ m L。本研究结果揭示 :母马脑垂体生长激素细胞和催乳激素细胞的数量和面积并不是决定母马这两种激素血中浓度的唯一重要因素 ;生长激素血中浓度上的个体差异 ,可能与其搏动性分泌形式有关 相似文献
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Yosuke Amagai Akane Tanaka Kyungsook Jung Akira Matsuda Kumiko Oida Sho Nishikawa Hyosun Jang Saori Ishizaka Hiroshi Matsuda 《Research in veterinary science》2014
Mast cell tumor (MCT) is the most common cutaneous tumor in dogs. We recently revealed that production of stem cell factor (SCF) contributes to the proliferation of neoplastic mast cells in an autocrine/paracrine manner. The aim of the present study was to determine the contribution of the mechanism in clinical MCTs. In consequence, high SCF expression (>10 times compared to HRMC cells) was observed in 5 of 7 MCT samples used in the study regardless of KIT mutation, which was confirmed in immunohistochemical analysis. In addition, production of SCF was observed in Ki-67-positive cells in the MCT xenograft. These results indicate the broad contribution of SCF autocrine/paracrine mechanism on clinical MCTs, providing the rationale for the clinical use of KIT inhibitors regardless of KIT mutation. 相似文献
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悬浮培养工艺与转瓶培养工艺的比较分析 总被引:2,自引:1,他引:1
采用反应器全悬浮培养BHK21细胞生产口蹄疫病毒与微载体悬浮培养Vero细胞生产狂犬病毒分别与相应的转瓶培养工艺生产案例对比分析,比较悬浮培养工艺与转瓶培养工艺的生产效益。分析显示,与转瓶培养工艺相比,反应器悬浮培养工艺获得的细胞密度、病毒效价、产品的产量和质量明显提高,生产时的能耗和劳动力需求明显降低。结果表明悬浮培养工艺的生产效益明显高于转瓶培养工艺,适宜于国内生物制品工业化生产的升级换代。 相似文献
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Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the germ cell lineage from ESCs/iPSCs is the key biological subject, since it would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro. Toward this goal, we recently established a culture system that induces functional mouse primordial germ cells (PGCs), precursors of all germ cells, from mouse ESCs/iPSCs. The successful in vitro production of PGCs arose from the study of pluripotent cell state, the signals inducing PGCs and the technology of transplantation. However, there are many obstacles to be overcome for the robust generation of mature gametes or for application of the culture system to other species, including humans and livestock. In this review, we discuss the requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs. 相似文献