共查询到20条相似文献,搜索用时 31 毫秒
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Takeshi Toyoda Jun-ichi Akagi Young-Man Cho Yasuko Mizuta Saeko Onami Isamu Suzuki Kumiko Ogawa 《Journal of toxicologic pathology》2013,26(2):215-221
To evaluate the potential role of DNA repair in bladder carcinogenesis, we performed an immunohistochemical analysis of expression of various DNA repair enzymes and γ-H2AX, a high-sensitivity marker of DNA double-strand breaks, in the urothelium of male F344 rats treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN), a bladder-specific carcinogen. Our results clearly demonstrated that γ-H2AX aggregation was specifically generated in nuclei of bladder epithelial cells of BBN-treated rats, which was not found in untreated controls or mesenchymal cells. γ-H2AX-positive cells were detected not only in hyperplastic and neoplastic areas but also in the normal-like urothelium after BBN treatment. These data indicate that γ-H2AX has potential as a useful biomarker for early detection of genotoxicity in the rat urinary bladder. To the best of our knowledge, this is the first report demonstrating expression of γ-H2AX during bladder carcinogenesis. 相似文献
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Gábor árpád CZIRJáK Lóránd Béla K?B?LKUTI Miklós TENK Attila SZAKáCS Attila KELEMEN Marina SP?NU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):701-703
A natural hybrid of Vipera ammodytes × Vipera berus was
presented having low body weight, seizures and generalized swelling of the cephalic
region. Based on the history of the case and clinical examination, hemorrhagic stomatitis
of traumatic origin was diagnosed. The snake was kept in a terrarium with wood chips as a
substrate, and the material had induced trauma in the oral mucosa which was further
complicated with Salmonella Arizonae and Morganella
morganii co-infection, abscessation and osteomyelitis. To the best of the
authors’ knowledge, this is the first reported case of bacterial infection in European
snake hybrids and one of a few case reports in European snakes. Although wood chips are an
inexpensive substrate, based on our findings, they should be avoided when keeping and
breeding European vipers. 相似文献
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《Journal of Equine Veterinary Science》2004,24(1):29-36
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Md. Masudur RAHMAN Erdenebelig UYANGAA Young Woo HAN Jin HUR Sang-Youel PARK John Hwa LEE Koanhoi KIM Seong Kug EO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):395-403
Newcastle disease (ND) is a highly contagious disease of chickens causing significant
economic losses worldwide. Due to limitations in the efficacy against currently
circulating ND viruses, existing vaccination strategies require improvements, and
incorporating immunomodulatory cytokines with existing vaccines might be a novel approach.
Here, we investigated the systemic and mucosal immunomodulatory properties of oral
co-administration of chicken interleukin-18 (chIL-18) and chicken interferon-α (chIFN-α)
using attenuated Salmonella enterica serovar Typhimurium on an
inactivated ND vaccine. Our results demonstrate that oral administration of S.
enterica serovar Typhimurium expressing chIL-18 or chIFN-α provided enhanced
systemic and mucosal immune responses, as determined by serum hemagglutination inhibition
antibody and NDV Ag-specific IgG as well as NDV Ag-specific IgA in lung and duodenal
lavages of chickens immunized with inactivated ND vaccine via the intramuscular or
intranasal route. Notably, combined oral administration of S. enterica
serovar Typhimurium expressing chIL-18 and chIFN-α significantly enhanced systemic and
mucosal immunity in ND-vaccinated chickens, compared to single administration of
S. enterica serovar Typhimurium expressing chIL-18 or chIFN-α. In
addition, oral co-administration of S. enterica serovar Typhimurium
expressing chIL-18 and chIFN-α provided enhanced NDV Ag-specific proliferation of
peripheral blood mononuclear cells and Th1-biased cell-mediated immunity, compared to
single administration of either construct. Therefore, our results provide valuable insight
into the modulation of systemic and mucosal immunity by incorporation of immunomodulatory
chIL-18 and chIFN-α using Salmonella vaccines into existing ND
vaccines. 相似文献
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Hellemans A Chiers K Decostere A De Bock M Haesebrouck F Ducatelle R 《Veterinary research communications》2007,31(4):385-395
‘Candidatus Helicobacter suis’ is a spiral-shaped bacterium that colonizes the stomach of more than 60% of slaughter pigs. The role of
‘Candidatus Helicobacter suis’ in gastric disease of pigs is still unclear. Experimental studies in pigs are lacking because this bacterium
is unculturable until now. An inoculation protocol using ‘Candidatus Helicobacter suis’ infected mouse stomach homogenate was used to reproduce the infection in pigs. Control animals were inoculated
using negative mouse stomach homogenate. Pigs were inoculated three times with one-week intervals and euthanized 6 weeks post
inoculation. Tissue samples were taken from different mucosal stomach regions to detect ‘Candidatus Helicobacter suis’ by PCR and urease test. Mucosal inflammation was evaluated on formalin-fixed tissue samples. Lesions in
the pars oesophagea were scored macroscopically. Infection was succesful in all challenged animals, with the antrum and the
fundus being predominantly positive. Infection was associated with infiltration of lymphocytes and plasma cells in the antral
mucosa, evolving to follicular gastritis. No apparent inflammation of the fundic stomach region was detected in the infected
animals. A clear link between ‘Candidatus Helicobacter suis’ and pars oesophageal lesions could not be found. 相似文献
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Bartosz WOJCIECHOWICZ Genowefa KOTWICA Justyna KOLAKOWSKA Anita FRANCZAK 《The Journal of reproduction and development》2013,59(1):49-58
Steroid hormones are produced by the porcine uterus. We hypothesized that the uterus in
pigs possesses active 3β-hydroxysteroid dehydrogenase/Δ5-Δ4
isomerase (3β-HSD) responsible for progesterone and androstenedione production, that
uterine steroids may supplement the amount of steroid hormones produced by embryos and
corpus luteum and that these steroids are necessary for maintenance of pregnancy. In this
study, we examined 1) endometrial and myometrial expression of 3β-HSD
mRNA, 2) uterine 3β-HSD protein activity and 3) in vitro production of
A4 and P4 by uterine slices harvested from pigs on days 10 to 11,
12 to 13 and 15 to 16 of pregnancy and the estrous cycle. The expression of
3β-HSD and the presence and activity of 3β-HSD protein were different
in the endometrium and the myometrium during the examined periods of pregnancy and the
estrous cycle. Production of A4 by the endometrium and myometrium was highest
on days 12 to 13 of pregnancy and the estrous cycle. Endometrial secretion of
P4 did not differ in the course of early pregnancy and on the respective days
of the estrous cycle. The gravid myometrium was the highest source of P4 in
pregnant pigs on days 12 to 13. The release of P4 by the cyclic myometrium rose
during the examined days of the estrous cycle. The steroidogenic activity of the uterus,
as described in this study, may support early pregnancy or the luteal phase of the estrous
cycle in pigs. 相似文献
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Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
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