中药口服液防治鸡新城疫的试验研究

将180只肉鸡随机分为6组,每组30只,即中药预防组与治疗组、西药预防组与治疗组、感染对照组、空白对照组。用贯叶连翘、香薷制成口服液,每只鸡每次口服1 mL,每天2次,连用5 d。中药口服液预防组存活率93.3%,治疗组存活率86.7%;金刚烷胺预防组存活率73.3%,治疗组存活率63.3%。中药口服液防治鸡新城疫,与金刚烷胺比较有较好的疗效。     

中药口服液防治鸡新城疫的试验

目的:研究分析中药口服液防治鸡新城疫的试验效果。方法:采取随机的方式把试验雏鸡划分四组,即A组(中药预防组)、B组(中药治疗组)、C组(西医预防组)以及D组(西医治疗组),各组均为30只,A组和B组应用中药口服液,C组和D组应用奥司他韦胶囊,观察四组雏鸡存活情况。结果:A组存活率为90.0%,B组存活率为83.3%,C组存活率为93.3%,D组存活率为86.7%,各组数据比较,差异不明显,不具有统计学意义(P0.05)。结论:应用中药口服液防治鸡新城疫,效果好。     

中药斑蝥口服液防治鸡新城疫的试验研究

用斑蝥制成口服液,每只鸡每次口服1mL,每天2次,连用5天。斑蝥口服液预防组存活率95.8%,治疗组存活率87.5%;金刚烷胺预防组存活率70.8%,治疗组存活率62.5%,斑蝥口服液防治鸡新城疫,与金刚烷胺比较有较好的疗效。     

中药蟾罗口服液防治鸡新城疫疗效观察

鸡新城疫（New castle disease,ND）又称亚洲鸡瘟、伪鸡瘟,是一种急性、高度接触性传染病。由于疫苗的广泛应用,使该病多以慢性或散发为特点。鸡新城疫发病后,抗生素、磺胺类药物无治疗作用,市售治疗鸡新城疫的中药大都是散剂,而鸡发病后食欲逐渐下降或废绝,但对饮欲影响较小。笔者用蟾罗口服液对人工感染鸡新城疫病进行了预防与治疗试验,蟾罗口服液预防组存活率96．1％,治疗组存活率88．4％,与金刚烷胺组比较有较好的疗效。     

复方黄芪、绞股蓝对鸡新城疫疫苗免疫效果的影响

以中药黄芪和绞股蓝为主制成口服液给接受鸡新城疫疫苗的鸡饮用，结果表明，中药组的鸡新城疫血凝抑制抗体效价（ＨＩ）、脾脏系数、法氏囊系数显著高于未饮用中药的对照组。中药口服液还拮抗地塞米松对鸡免疫机能的抑制作用，     

中药口服液防治鹅副黏病毒病的试验研究

用珍珠草、华荠苧2味中药制成口服液,研究其防治鹅副黏病毒病的效果。将试验雏鹅随机分成6组,其中中药预防与治疗组每羽每次口服1 m L,2次/d,连用4 d。西药奥司他韦(达菲)胶囊预防与治疗组每羽每次口服18.75mg,2次/d,连用4 d。结果中药口服液预防组存活率90%,治疗组存活率80%;西药预防组存活率95%,治疗组存活率80%。表明中药口服液防治鹅副黏病毒病的疗效与奥司他韦基本相同。     

中药蟾甘口服液鸡胚接种抗鸡新城疫病毒研究

用鸡胚培养法和红血球凝集(HA)试验,测定中药蟾甘口服液对鸡新城疫病毒的抑制作用。试验共分4组,第Ⅰ组接种病毒尿囊液与中药蟾甘口服液混合液,HA滴度0。第Ⅱ组接种病毒尿囊液与西药金刚烷胺混合液,HA滴度5log2。第Ⅲ组接种病毒尿囊液与生理盐水混合液,HA滴度9log2。第Ⅳ组不接种病毒尿囊液,不给药,HA滴度0。结果表明,中药蟾甘口服液对鸡胚无毒性作用。中药蟾甘口服液与病毒同时接种鸡胚,能完全抑制鸡新城病毒在鸡胚中增殖。     

参莲口服液治疗猪、鸡腹泻临床疗效观察

为考察参莲口服液(由苦参、穿心莲等中药按一定比例提取而成)对动物腹泻的防治效果,开展了参莲口服液对架子猪、肉鸡腹泻临床防治试验。结果高、中、低剂量组与空白对照组治愈率差异显著(P0.05),中、高剂量组与空白照组差异极显著(P0.01),中、高剂量组之间无显著性差异(P0.05)。试验证明单独使用参连口服液治疗畜禽腹泻疗效确切。     

新囊素口服液的检测方法

新囊素口服液的检测方法祁彦战石徐志华王丽１王权２吉林省兽药饲料监察所长春１３００６２；１长春市朝阳区农业局；２吉林省植保站新囊素口服液〔１〕是一种清热解毒燥湿利水的复方中药合剂，用于防治鸡新城疫、鸡传染性法氏囊炎及肠道疾病。在临床应用中取得了效果。为...     

新中兽药"加味双黄汤口服液"的体外抑菌试验

中药复方的组成必须具有科学性、合理性 ,忌凭主观臆断组方、盲目配伍。加味双黄汤口服液是针对畜禽中细菌性和病毒性疾病的特点 ,严格按照传统中兽医理论选药配方。同时结合现代药理学及毒理学的先进研究手段 ,研制开发的一组由银花、连翘等数十味中药组成的中兽药新制剂。在临床治疗鸡慢性呼吸道病、鸡白痢、鸡新城疫及畜禽下痢等急性传染病取得满意的临床效果。为进一步证实该产品的抑菌效力 ,为研制开发应用新中兽药加味双黄汤口服液提供确切的科学理论依据 ,现将加味双黄汤体外抑菌试验结果报道如下。1　材料与方法1.1　试验材料1.1.1…     

中药制剂对禽流感病毒H9N2、新城疫病毒、传染性支气管炎病毒和鸡毒支原体的攻毒保护试验

采用以野菊花、穿心莲、柴胡等10味中药提取的中药制剂,连续给22日龄SPF鸡口服5 d后,以禽流感病毒H9N2、新城疫病毒F4、传染性支气管炎病毒强毒株M41和支原体S6强毒分别攻击,观察SPF鸡临床症状、发病率、病理学变化和分离病毒。结果表明,此中药制剂75 mg/只口服组,新城疫病毒、禽流感病毒和传染性支气管炎病毒攻击后发病率分别为12.5%,0%,10%;37.5 mg/只剂量口服组发病率分别为50%,10%,10%,且低剂量组病毒分离阳性。攻击支原体后,以上两个剂量组SPF鸡病变指数分别3.5和5.5,研究证实本中药制剂可以控制以上4种病原所致的家禽呼吸道病的发生。     

黄芪、白术水煎剂对鸡新城疫疫苗免疫效果的影响

探讨鸡口服黄芪、白术水煎剂对鸡新城疫疫苗免疫效果的影响。给7日龄健康梅岭土鸡连续7 d饮用口服黄芪或白术水煎剂,12.5 g/kg.d,后分2次间隔14 d滴鼻接种鸡新城疫Ⅳ系弱毒苗(LaSota株)。分别在首免前、及免疫后1、2、3、4周采血,分离血清,用血凝抑制试验检测鸡新城疫抗体效价(HI)。结果显示,口服中药组的鸡血清HI效价高于未服中药对照组,黄芪水煎剂组于二免后1周,白术水煎剂组于二免后1、2周,HI效价显著高于未服中药对照组。结果表明,在疫苗免疫之前给鸡口服白术水煎剂或黄芪水煎剂可以显著增强免疫效果。     

复方中药体外抗新城疫病毒活性研究

为了筛选出高效的抗新城疫病毒中药,采用鸡胚接种试验,对8种经典清热解毒复方中药的抗新城疫病毒效果进行了测定。结果表明,荆防败毒散、银翘散、白头翁汤、双黄连口服液在体外对新城疫病毒具有较好的抑制作用。     

复方金银花对鸡体内NDV分布的影响

选用1日龄雏鸡,无污染环境饲养21 d后,随机分成感染组、中药治疗组、空白组。中药治疗组饮水给予复方金银花制剂。以单克隆抗体介导的免疫组化（IHC）法检测攻毒鸡体内NDV抗原的分布及定位,以确定复方金银花对新城疫病毒感染鸡的组织嗜性的影响。结果显示,感染组和中药治疗组鸡的16个组织器官中均能检测到NDV抗原,病毒以气管、肺脏、十二指肠、空肠等器官中分布为最高。复方金银花对新城疫的组织损伤有一定的保护作用,可缩短组织修复的时间。     

复方中草药饲料添加剂对黄羽肉鸡免疫性能的影响

以1日龄商品代苏禽黄鸡为试验素材,随机分成3个处理组,每组50只。组1为添加抗生素组,组2为添加自配复方中草药组,组3为对照组。试验结果表明：添加复方中草药有提高胸腺、脾脏、法氏囊重量及其指数的趋势,其中第3周末,中草药组的脾脏重量及其指数显著高于抗生素组和对照组;试验组在免疫新城疫疫苗7 d后,血清中的抗体效价均显著高于对照组,这种差异中草药组一直持续到本次试验免疫后的第21 d。     

Effects of the Oral Liquid of Astragalus and Lucidum Polysaccharides on Immunological Function in Chickens Immuned with ND Vaccine

In order to study the effects of the oral liquid of Astragalus and Lucidum polysaccharides on immunological function in chickens,one hundred and eighty 14-day-old chickens were randomly divided into 9 groups and inoculated with the Newcastle disease vaccine.1 to 3 experimental groups were respectively the oral liquid of Astragalus and Lucidum polysaccharides high,middle and low dose groups;4 to 6 experimental groups were respectively the oral liquid of Astragalus and Lucidum polysaccharides with cyclophosphamide high,middle and low dose groups;7 to 9 experimental groups were respectively cyclophosphamide group,positive control group and saline control group.The proliferation index of spleen lymphocyte and Newcastle disease virus antibody level by HI method were determined on days 7,14,21 and 28 after vaccination.The results showed that the oral liquid of Astragalus and Lucidum polysaccharides could apparently promote lymphocyte proliferation and enhance antibody titer,and alleviate the immunosupperession of the chickens in some degree,the low dose of the oral liquid of Astragalus and Lucidum polysaccharides promoted lymphocyte proliferation best,implying that the oral liquid of Astragalus and Lucidum polysaccharides would be expected as a candidate of new-type immunopotentiator.     

黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响

为研究黄芪灵芝口服液对雏鸡免疫功能的影响,本试验将180只14日龄雏鸡随机分成9组并通过点眼滴鼻免疫新城疫(ND)疫苗。1~3组分别是黄芪灵芝口服液高、中和低剂量组;4~6组分别是环磷酰胺+黄芪灵芝口服液高、中和低剂量组;7~9组分别为环磷酰胺组、阳性对照组及生理盐水对照组。分别于免疫后的第7、14、21、28天测定脾脏淋巴细胞增殖和抗体滴度的动态变化。结果表明,黄芪灵芝口服液能提高雏鸡脾脏淋巴细胞的增殖能力和促进新城疫病毒(NDV)抗体的产生,能一定程度上缓解环磷酰胺的免疫抑制,其中黄芪灵芝口服液低剂量组对脾脏淋巴细胞增殖效果最好,黄芪灵芝口服液可作为一种新型的免疫增强剂。     

应用鸡胚接种试验研究中草药的抗新城疫病毒作用

为了筛选出高效的抗新城疫病毒(Newcastle disease virus,NDV)中草药,采用鸡胚接种试验测定了常用清热解毒中草药的体外抗NDV作用.结果表明矮地茶、侧柏叶、贯众、诃子、虎杖、白蔹、夏枯草、黄连对新城疫病毒具有较好的杀灭作用.     

Serological response of chickens to oral vaccination with Newcastle disease virus

Conventional Newcastle disease vaccines are not suitable for application to village chickens in tropical countries of Asia. Trials with food-based vaccines are being initiated and the following experiments were performed to evaluate oral vaccination with Newcastle disease virus. Experimental chickens were vaccinated orally with the avirulent V4 strain of Newcastle disease virus and haemagglutination-inhibition antibody responses were measured. V4 virus was introduced into the crop by tube and total faecal output was collected daily and assayed for Newcastle disease virus. Virus was recovered on Days 5 and 6 after vaccination from most chickens that had received 10(7.4) and 10(6.4) 50% egg-infectious doses (EID50) of virus. There was no recovery of virus from birds receiving a lower dose of vaccine. Groups of chickens kept in cages with wire floors were given various doses of vaccine into the crop. Higher antibody titres were achieved with higher doses of virus. This dose responsiveness was not observed when various doses of vaccine were presented on food pellets and the groups of chickens were kept on concrete floors. Similar antibody responses were then seen with nominal doses of 10(5.2) and 10(8.2) EID50 per bird, possibly as a result of excretion and re-ingestion of the vaccine virus. Spread of the vaccine virus was demonstrated when control chickens and chickens receiving 10(7.7) EID50 of V4 virus on food pellets were housed together on a concrete floor. Similar antibody titres were achieved in both vaccinated and in-contact chickens.     

日粮中添加不同剂量中草药对蛋鸡新城疫油乳剂灭活疫苗免疫效果的影响

本试验旨在研究日粮中添加不同剂量中草药对蛋鸡新城疫油乳剂灭活疫苗免疫效果的影响。选用240只100日龄海兰褐蛋鸡,随机分为4组,每组5个重复,每个重复12只。对照组饲喂基础日粮,不添加任何添加剂;试验Ⅰ、Ⅱ和Ⅲ组分别在基础日粮中添加0.5%、1.0%和1.5%中草药添加剂。结果表明,试验Ⅱ和Ⅲ组蛋鸡血清中新城疫抗体效价、雏鸡血清中新城疫母源抗体效价均显著高于对照组和试验Ⅰ组（P＜0.05）,且试验Ⅱ和Ⅲ组、对照组和试验Ⅰ组之间均无显著差异（P>0.05）。提示,在免疫注射疫苗的同时日粮中添加1.0%或1.5%中草药添加剂,可提高蛋鸡新城疫抗体效价,增强机体的免疫力。