Intercropping for the Improvement of Sorghum Yield, Soil Fertility and Striga Control in the Subsistence Agriculture Region of Tigray (Northern Ethiopia)

Striga hermonthica is a major biotic constraint in the dry and less fertile areas of northern Ethiopia. Emphasis is being placed on improved cropping systems to address the interrelated problems of Striga and soil fertility decline. The potential benefits of intercropping were investigated at two sites representing different environments for crop yield improvement, soil fertility maintenance and Striga control. Ten food legume and oilseed crop species were compared in inter‐row arrangement with sorghum under non‐fertilized conditions. In most cases, there was no significant negative impact of intercropping on sorghum growth and development. Among the intercrops, two cowpea varieties – cv. TVU 1977 OD and cv. Blackeye bean – produced the highest supplemental yield of up to 329 and 623 kg ha^?1 grain and 608 and 1173 kg ha^?1 biomass at Adibakel and Sheraro respectively. Treatment differences on Striga infestation and measured soil fertility indicators were not significant. Nevertheless, valuable grain and biomass obtained from the legume intercrops, without seriously compromising sorghum yield, could offer multiple benefits as a source of protein, additional income, feeds for animals and manure in the subsistence agriculture regions of northern Ethiopia.     

Efficiency of secondary traits in selecting for improved grain yield in extra‐early maize under Striga‐infested and Striga‐free environments

A base index involving Striga damage, number of emerged Striga plants and ears per plant is used for selecting for maize (Zea mays L.) grain yield under Striga infestation. There are contradictory reports on the reliability of number of emerged Striga plants for selecting for Striga resistance. The objective of this study was to confirm reliability of the secondary traits for selecting for improved grain yield under Striga infestation. Ten Striga‐resistant extra‐early cultivars were evaluated for 3 years under artificial Striga‐infested and Striga‐free environments in Nigeria. Analysis of variance combined across years and locations showed significant mean squares for genotype, year, location and their interactions for most traits. Sequential path analysis identified ear aspect as the only trait with significant direct effect on yield under artificial Striga infestation, while GGE biplot confirmed ear aspect, ears per plant and Striga damage as the most reliable traits. Ear aspect should be included in the base index for selecting for improved grain yield of extra‐early maize under Striga infestation, while the number of emerged Striga plants should be excluded.     

Characterization of host tolerance to Striga hermonthica

Summary One of the most promising control options against the parasitic weed Striga hermonthica is the use of crop varieties that combine resistance with high levels of tolerance. The aim of this study was to clarify the relation between Striga infestation level, Striga infection level and relative yield loss of sorghum and to use this insight for exploring the options for a proper screening procedure for tolerance. In three pot experiments, conducted in Mali (2003) and The Netherlands (2003, 2004), four sorghum genotypes were exposed to a range of Striga infestation levels, ranging from 0.0625 to 16 seeds cm⁻³. Observations included regular Striga emergence counts and sorghum grain yield at maturity. There were significant genotype, infestation and genotype × infestation effects on sorghum yield. The relation between infestation level and infection level was density dependent. Furthermore, the relation between Striga infection level and relative yield loss was non-linear, though for the most resistant genotype Framida only the linear part of the relation was obtained, as even at high infestation levels only moderate infection levels were achieved. The results suggest that for resistant genotypes, tolerance can best be quantified as a reduced relative yield loss per aboveground Striga plant, whereas for less resistant genotypes the maximum relative yield loss can best be used. Whether both expressions of tolerance are interrelated could not be resolved. Complications of screening for tolerance under field conditions are discussed.     

Screening of Ethiopian sorghum (Sorghum bicolor) landraces for their performance under Striga hermonthica ‐infested conditions

Striga hermonthica is a major constraint to sorghum production and productivity in sub‐Saharan Africa, Ethiopia, in particular. The objectives of this study were to evaluate the performance of 49 sorghum genotypes based on their reaction to S. hermonthica and investigate the relationships among yield and Striga resistance traits, including the Striga emergence count, area under Striga number progress curve, area under Striga severity progress curve, grain yield under infested, grain yield under non‐infested conditions and relative yield loss. The genotypes differed significantly in all measured parameters under Striga‐infested and non‐infested conditions. The genotypes ‘Birhan’, ‘Gubiye’, Wolegie, Zegerie, Nechmashila I, Woftel, Tetron and Eyssa were identified as promising ones based on grain yield and Striga‐related traits. On the other hand, the genotypes Jamyo, Bobie, Gedido, Mankebar and Zengada had moderate Striga numbers with low relative yield loss as compared with susceptible checks. The most promising variety, Zegerie, Mankebar and Zengada, out yielded the standard resistant checks ‘Birhan’ and ‘Gubiye’ under Striga infestation. Ward cluster analysis grouped the 49 sorghum genotypes in to four distinct clusters under Striga‐infested conditions. All members of clusters II and III showed the highest yielding group with the lowest to moderate Striga number, while cluster IV supported the lowest yield with the highest Striga number. Significant negative correlations were observed between yield‐ and Striga‐related traits. Highly significant and strong correlations were observed among Striga resistance indices, indicating that any of the Striga resistance parameters can be used as an indicator of resistance. The wide variations in grain yield among genotypes under Striga‐infested conditions would be invaluable genetic resources for production in Striga endemic areas of Ethiopia.     

Effect of Striga hermonthica on the Growth Characteristics of Sorghum Intercropped with Groundnut Varieties

Field experiments were conducted in 1996 and 1997 on a Striga‐infested field to determine the effect of Striga on the growth characteristics of sorghum intercropped with groundnut varieties (RMP‐12, Yarkasa and Ex‐Dakar). The performance of the intercropped sorghum was significantly better than that of the monoculture in terms of plant height, dry matter, leaf number and leaf area index. Intercropping of sorghum with RMP‐12 resulted in a significant suppression of Striga compared to other groundnut varieties. The dry matter yield of sorghum intercropped with groundnut was significantly higher than that of the sorghum monocrop. The dry matter yield resulting from intercropping of sorghum with the RMP‐12 groundnut variety was significantly higher than that of sorghum intercropped with all other varieties of groundnut.     

Inheritance of Striga hermonthica adaptive traits in an early‐maturing white maize inbred line containing resistance genes from Zea diploperennis

Striga hermonthica can cause as high as 100% yield loss in maize depending on soil fertility level, type of genotype, severity of infestation and climatic conditions. Understanding the mode of inheritance of Striga resistance in maize is crucial for introgression of resistance genes into tropical germplasm and deployment of resistant varieties. This study examined the mode of inheritance of resistance to Striga in early‐maturing inbred line, TZdEI 352 containing resistance genes from Zea diploperennis. Six generations, P₁, P₂, F₁, F₂, BC₁P₁ and BC₁P₂ derived from a cross between resistant line, TZdEI 352 and susceptible line, TZdEI 425 were screened under artificial Striga infestation at Mokwa and Abuja, Nigeria, 2015. Additive‐dominance model was adequate in describing observed variations in the number of emerged Striga plants among the population; hence, digenic epistatic model was adopted for Striga damage. Dominance effects were higher than the additive effects for the number of emerged Striga plants at both locations signifying that non‐additive gene action conditioned inheritance of Striga resistance. Inbred TZdEI 352 could serve as invaluable parent for hybrid development in Striga endemic agro‐ecologies of sub‐Saharan Africa.     

Screening Maize for Tolerance to Striga hermonthica

Striga hermonthica is a widespread parasitic weed in Africa, causing severe damages to the major cereal crops: millet, sorghum and maize. Considering the subsistence nature of farming in Africa, the development of resistant or tolerant varieties is the most promising cost-effective approach to overcoming the weed problem. A simple, inexpensive and sufficiently uniform infestation technique has been developed by the research department of AgSeeds Ltd., Zaria, Nigeria, which is based on mulching a field with mature Striga plants collected in neighbouring farmers' sorghum fields. With this method, rapid progress was achieved in the development of experimental maize hybrids with high levels of tolerance to S. Hermonthica and good yield potential. Among 34 lines with improved tolerance, two (nos. 74 and 785) showed particularly high levels of tolerance. The hybrid between these two lines had a significantly higher yield (47.6 %) under Striga infestation than the tolerant check 8322-13 developed by IITA.     

Crop Rotation to Improve Agricultural Production in Sub-Saharan Africa

A three years' trial was conducted in a farmers' field in northern Ghana to evaluate the effect of sole crops (cotton, cowpea, groundnut, soybean, and sunflower) planted once or twice on yield of the staple foods of the region, maize and sorghum. Sole cropping for only one year already resulted in significant yield increases for maize and partly for sorghum compared to the conventional cropping of mixed stands of maize–sorghum or maize–groundnut and natural fallow. Lowest yield of maize and sorghum was obtained where these cereals followed maize–sorghum (monoculture). Intercropping of maize with groundnut led to subsequent maize and sorghum yields which were similar to those obtained after maize–sorghum. After growing legumes and sunflower for one year the grain and straw yield of maize and sorghum was significantly higher in the two consecutive years than after cereal (maize–sorghum) monoculture. In this trial maize and sorghum were found to be not as tolerant to the disadvantages of monoculture or preceding cereals–legumes mixture. The results suggest that continuous intercropping with cereals under the given conditions has negative effects on soil fertility and can lead to an increase in soil-borne pests and troublesome weeds like Striga comparable to monocropped cereals.     

Soil Organic Carbon Build-up and Dynamics in Rice–Rice Cropping Systems

Intensive irrigated rice system is the most important food production system in the world. Continued population growth necessitates increased rice production to meet the increased need of food production through increased rice productivity to ensure food security. In contrast, the recent slowdown in yield growth in intensive irrigated rice‐based cropping systems as a result of deterioration of soil health and decline in productivity level is a serious cause for concern. The objective of this research was to investigate the soil organic carbon (SOC) build‐up and its dynamics in rice–rice cropping system by including a green manure (GM) crop Sesbania rostrata Berm. either during fallow and/or intercrop at 4 : 1 ratio as additive series without changing rice geometry. The results revealed that there is a gradual build‐up of SOC when S. rostrata included and in situ incorporated at flowering stage as a basic means of improving soil quality in rice–rice cropping system. Further, the soil organic matter fractions viz. humic acid and fulvic acid were also improved in all GM‐incorporated systems. In general, the proportion of fulvic acid was higher than humic acid. Cropping system involving three GMs viz. S. rostrata‐rice/S. rostrata‐rice/S. rostrata registered significantly higher SOC and was 10.63 percent higher than traditional rice–rice cropping system (A₁). This was followed by S. rostrata‐rice/S. rostrata‐rice system. Although the traditional rice–rice cropping system (A₁) also conserved SOC (0.29 and 1.69 % higher than initial during first and second annual cropping cycle, respectively), the magnitude was very low. Repeated application of S. rostrata as GM improved SOC, which formed the basis for sustainable management of soil resources. It can be concluded that S. rostrata would affect the rate of loss of SOC in cropping systems and its ultimate level in soils.     

Effects of Nitrogen on Dry Matter Accumulation and Productivity of Three Cropping Systems and Residual Effects on Wheat in Deep Vertisols of Central India

A field experiment was conducted on deep vertisols of Bhopal, India to study the effects of three levels of nitrogen (N), namely 0, 75 and 100 % of the recommended dose of nitrogen (RDN), on the dry matter accumulation (DMA) and productivity of three cropping systems (sole soybean, sole sorghum and soybean + sorghum intercropping) during the rainy season and their residual effect on the subsequent wheat crop during the post-rainy season. During the rainy season, sole sorghum was found to have significantly higher DMA and productivity in terms of soybean equivalent yield (SEY) than sole soybean or soybean + sorghum intercropping. Increasing the N dose from 0 to 100 % RDN significantly improved the DMA and SEY. At a low fertility level (N₀), soybean + sorghum intercropping was found to be more productive, while at a high fertility level (100 % RDN), sole sorghum was more productive than the other two cropping systems. However, during the post-rainy season, sole soybean as the preceding crop gave the highest DMA and seed yield of wheat, which were similar to those found with soybean + sorghum intercropping. Sorghum followed by wheat gave the lowest DMA and seed yield of wheat. Application of 100 % RDN irrespective of cropping system during the preceding crop improved the DMA of wheat but not its seed yield. However, N applied to the wheat crop significantly increased its DMA and seed yield.     

Assessing the suitability of stress tolerant early-maturing maize (Zea mays) inbred lines for hybrid development using combining ability effects and DArTseq markers

Identification of hybrids for commercialization is crucial for sustainable maize production in sub-Saharan Africa (SSA). One hundred and ninety test crosses, 10 tester × tester crosses + 10 hybrid checks were evaluated across 11 environments, 2017 to 2019. Inheritance of grain yield under Striga infestation, optimal and across environments was influenced by additive genetic action, but there was greater influence of nonadditive gene action under drought stress conditions. Nine, seven and two inbreds had significant and positive general combining ability (GCA) effects for grain yield under Striga-infested, optimal and drought stress environments, respectively, and would contribute high grain yield to their progenies. Heterotic grouping methods based on specific and GCA, GCA effects of multiple traits and DArTseq markers classified the inbreds into five, three and two heterotic groups, respectively, across research conditions. The DArTseq markers method that classified the inbred lines into two major heterotic groups and was one of the most efficient methods should be adopted for practical purposes in maize breeding programmes in SSA. Hybrids TZEI 7 × TZdEI 352, TZEI 1238 × TZEI 7 and TZEI 1252 × TZEI 7 had outstanding grain yield under contrasting environments and should be tested on-farm for commercialization in SSA.     

Assessment of reactions of diverse maize inbred lines to Striga hermonthica (Del.) Benth

Striga hermonthica is the most widespread and destructive obligate root parasite infecting maize and other cereals in Africa. Maize inbred lines supporting reduced S. hermonthica emergence can form an important basis for developing Striga‐resistant maize cultivars. Twenty new inbred lines selected for field resistance to S. hermonthica, and five inbred checks with known resitance, tolerance and susceptibility reactions to S. hermonthica were evaluated in pots, greenhouse and field experiments under artificial Striga infestation for 3 years. The experiments were conducted to determine the extent of variation in parasite attachment to the roots of these lines and its relationship with emerged Striga plants and other traits. Significant differences (P < 0.0001) were detected among the inbred lines for the numbers of attached and emerged Striga plants and the results were consistent across test environments. Also, the lines exhibited significant differences for Striga damage symptom ratings and other traits recorded in the field. Parasite attachment to the roots was significantly correlated with emerged Striga count in the screenhouse (r = 0.67–0.68, P < 0.001) and in the field (r = 0.82–0.84, P < 0.0001) and with levels of grain yield reduction due to Striga (r = 0.71, P < 0.0001). Regression analysis of the numbers of attached parasites on the first principal component axis scores that integrated several traits recorded in the field was significant (P < 0.0001) and accounted for 62% of the total variation in numbers of attached parasites. The new inbred lines and the resistant inbred check were the least affected by S. hermonthica and exhibited yield losses of 0–37% compared with the yields of the tolerant and the susceptible inbred checks, which were reduced by 40–85%. Sixteen new inbred lines supported significantly fewer attached parasites compared with the susceptible inbred check. Some of these lines also supported significantly fewer emerged parasites and sustained lower damage symptoms and percentage yield losses due to Striga compared with the susceptible inbred check. These inbred lines would be useful in breeding programmes for developing resistant maize cultivars.     

CO<Subscript>2</Subscript>-assimilation and chlorophyll fluorescence as indirect selection criteria for host tolerance against <Emphasis Type="Italic">Striga</Emphasis>

Striga hermonthica (Del.) Benth. is a parasitic weed on tropical cereals causing serious yield losses in Africa. The use of host crop varieties with improved resistance and tolerance against this parasite is a key component of an integrated control strategy. Breeding for tolerance is however seriously hampered by the absence of reliable and yet practical selection measures. The observation that the photosynthetic rate of tolerant genotypes is less sensitive to Striga infection was used as a starting point to search for suitable selection measures. In a greenhouse pot experiment the effect of Striga infection on the photosynthesis of four sorghum (Sorghum bicolor [L.] Moench) genotypes, differing in Striga tolerance level, was measured at three moments in time (26, 48 and 75 days after sowing). Genotypes were CK60-B, E36-1, Framida and Tiémarifing. Measurements involved CO₂-assimilation (A) and three chlorophyll fluorescence characteristics (electron transport rate through photosystem II [ETR], photochemical [Pq] and non-photochemical quenching [NPq]). Striga infection negatively affected A, ETR and Pq. Based on A and Pq, genotypes with superior levels of tolerance (Tiémarifing) could be discriminated from genotypes with superior level of resistance (Framida). Both A and Pq showed high heritabilities and consequently clear and predictable differences between genotypes. Using discriminative ability, heritability and cost effectiveness as main criteria, photochemical quenching (Pq) was concluded to possess the highest potential to serve as indirect selection measure for host plant tolerance to Striga. Screening should preferably be conducted at relatively high Striga infestation levels, between Striga emergence and host plant flowering.     

秸秆还田对豆-稻-菜循环耕作模式下作物产量和土壤肥力的影响

豆-稻-菜循环耕作模式是中国南方地区最重要的轮作模式之一,但秸秆还田对该耕作制度下作物产量和土壤肥力的影响尚不明确。通过连续7年的田间定位试验（2009—2015年）,研究秸秆还田对豆-稻-菜循环耕作模式下作物产量和土壤养分状况的影响。结果表明：秸秆还田对浙江省豆-稻-菜轮作模式下作物产量的影响显著：与单施化肥相比,秸秆还田有效提高了水稻和小萝卜的产量,且水稻和小萝卜年均增产10%以上;此外,秸秆还田对豆-稻-菜循环种植区域土壤肥力也有较大影响：提高了土壤有机质、全氮、有效磷、速效钾含量和土壤pH值,此外,秸秆还田区域土壤全氮、有效磷和速效钾含量呈逐年增加趋势。由此可见,多熟制耕作制度下秸秆还田仍具有作物增产和土壤培肥作用,可作为耕地质量提升的有效手段长期实施。     

Genetic effect of <Emphasis Type="Italic">Striga</Emphasis> resistance in sorghum genotypes

Striga is an important parasitic weed causing substantial economic losses in cereal and legume crop production in sub-Saharan Africa. Integrated Striga management approaches such as a combined use of Striga resistant varieties and Fusarium oxysporum f.sp. strigae (FOS), a biocontrol agent of Striga, are an option to control the parasite and to boost sorghum productivity. Understanding host gene action influencing Striga resistance, with or without FOS treatment, is key to developing improved sorghum varieties with durable resistance and high yield. The objective of this study was to determine the gene action and inheritance of Striga resistance using genetically diverse populations of sorghum involving FOS treatment. Twelve sorghum parents selected for Striga resistance, FOS compatibility or superior agronomic performances were crossed using a bi-parental mating scheme. The selected male and female parents and their F₁ progenies, backcross derivatives and the F₂ segregants were field evaluated at three locations in Tanzania known for their severe Striga infestations using a lattice experimental design with two replications. The following data were collected and subjected to generation mean analysis (GMA): days-to-50% flowering (DFL), seed yield per plant (SYP) and number of Striga per plant (SN). GMA showed the preponderance of additive genetic action contributing to the total genetic variation in the evaluated sorghum populations. The additive genetic effect for DFL, SYP and SN, with and without FOS treatments, ranged from 72.02 to 86.65% and 41.49 to 95.44%, 75.62 to 91.42% and 71.83 to 91.89%, and 77.35 to 93.56% and 72.86 to 95.84%, in that order. The contribution of non-additive genetic effects was minimal and varied among generations. FOS application reduced DFL and SN and improved SYP in most of the tested sorghum populations. DFL of sorghum populations was reduced by a mean of 8 days under FOS treatment compared to the untreated control in families such as 675 × 654, AS435 × AS426 and 1563 × AS436. FOS treatment improved SYP with a mean of 6.44 g plant^?1 in 3424 × 3993 and 3984 × 672. The numbers of Striga plants were reduced with a mean of 16 plants due to FOS treatment in the crosses of 675 × 654, 1563 × AS436, 4567 × AS424, and 3984 × 672. The study demonstrated that additive genes were predominantly responsible for the inheritance of Striga resistance in sorghum. Pure line cultivar development targeting reduced DFL, SN and high SYP in the selected populations may provide enhanced response to selection for integrated Striga management (ISM) programme.     

Field evaluation of mixed-seedlings with rice to alleviate flood stress for semi-arid cereals

Flash floods, erratically striking semi-arid regions, often cause field flooding and soil anoxia, resulting in crop losses on food staples, typically pearl millet (Pennisetum glaucum L.) and sorghum (Sorghum bicolor (L.) Moench). Recent glasshouse studies have indicated that rice (Oryza spp.) can enhance flood stress tolerance of co-growing dryland cereals by modifying their rhizosphere microenvironments via the oxygen released from its roots into the aqueous rhizosphere. We tested whether this phenomenon would be expressed under field flood conditions. The effects of mix-planting of pearl millet and sorghum with rice on their survival, growth and grain yields were evaluated under controlled field flooding in semi-arid Namibia during 2014/2015–2015/2016. Single-stand and mixed plant treatments were subjected to 11–22 day flood stress at the vegetative growth stage. Mixed planting increased plant survival rates in both pearl millet and sorghum. Grain yields of pearl millet and sorghum were reduced by flooding, in both the single-stand and mixed plant treatments, relative to the non-flooded upland yields, but the reduction was lower in the mixed plant treatments. In contrast, flooding increased rice yields. Both pearl millet–rice and sorghum–rice mixtures demonstrated higher land equivalent ratios, indicating a mixed planting advantage under flood conditions. These results indicate that mix-planting pearl millet and sorghum with rice could alleviate flood stress on dryland cereals. The results also suggest that with this cropping technique, rice could compensate for the dryland cereal yield losses due to field flooding.     

Quantitative-genetic parameters of sorghum growth under striga infestation in Mali and Kenya

To estimate quantitative‐genetic parameters of sorghum for resistance to the hemi‐parasitic weed striga [Striga hermonthica (Del.) Benth.] and for agronomic traits, 36 diallel F₂ populations and their nine parental lines were evaluated under severe striga infestation at two locations each in Mali and Kenya. Location means for grain yield ranged from 132 to 254 g/m². F₂ populations outyielded lines on average by 18%. For striga emergence traits, F₂ heterosis values ranged from ‐36% to 232% among populations. Genetic and genotype x environment interaction variances of lines and F₂s were highly significant for all traits. Broad‐sense heritabilities for areas under striga severity progress curves and grain yield were 0.83 and 0.90 in lines, and 0.81 and 0.89 in F₂s, respectively. General and specific combining ability, and their interaction effects with locations were significant for most traits. F₂ superiority for grain yield under striga infestation demonstrates the potential merit of heterozygous cultivars in the target areas. Significant genotype x environment interaction entails multilocational testing to identify stable resistance. A combination of resistance with striga tolerance is recommended to breeders.     

Screening techniques and improved biological nitrogen fixation in cool season food legumes

Dinitrogen fixation and legume productivity are greatly influenced through the interactions of legume host, Rhizobium, and the above- and below-ground environment. The benefits of improving legume N₂ fixation include reduced reliance on soil N, leading to more sustainable agricultural systems and reduced requirements for fertilizer N, enhanced residual benefits to subsequent crops, and increased legume crop yields. Most of the gains in N₂ fixation to date have been derived from management of legume cropping systems and through inoculation of legume seed with competitive and symbiotically effective rhizobia. Further gains are possible by developing plant cultivars with tolerance to soil abiotic factors, increased plant yield, and a broader and more effective matching of plant host and rhizobia. Techniques for screening material for superior N₂ fixation and examples of programs to increase fixed N, with attention to the major abiotic stresses, are discussed.     

Genotypic yield responses of lowland rice in high-altitude cropping systems

Rising global mean temperatures open opportunities in high-altitude production systems for temperature-sensitive crops such as lowland rice. Currently, the cropping window for rice in higher altitudes is still narrow, and thus, genotypes that tolerate a certain degree of chilling are needed to achieve their potential yield. Final yield depends on the interaction between genotype and environmental conditions. Exposing the genotype to a wide range of environments is a way to evaluate its adaptability into an expanding cropping calendar. Over a 2-year period, an experiment was conducted in lowland rice systems in Madagascar at two locations differing in altitude. Twenty genotypes with contrasting levels of tolerance to low temperature were sown monthly in a non-replicated rice garden trial. Plant development was monitored and yield and yield components were determined. Yield stability across the different growing environments was investigated. While crop duration was affected by sowing dates and altitude, yield was mainly determined by sowing date. Panicle number per m² and number of spikelets per panicle were the most limiting factors for yield potential in mid-altitude, while in high altitude, yield was mainly limited by spikelet fertility. Resulting cropping calendar and genotype recommendations are discussed.     

连作对甘肃中部沿黄灌区马铃薯干物质积累和分配的影响

甘肃省中部沿黄灌区是全国重要的加工型马铃薯生产基地, 因集约化种植带来连作障碍问题已严重影响到农业产业的健康发展, 所以探索连作对马铃薯块茎产量形成的影响与机理十分必要。根据田间定位试验, 以前茬为玉米的地块作对照,设连作1~5年处理, 采用常规方法,调查连作对马铃薯产量、干物质积累和分配以及花后干物质积累和转运的影响。结果表明, 与对照相比, 短期(1~2年)连作未表现连作障碍现象, 连作第3年, 块茎产量显著下降, 降幅达21.68%~75.67%, 单薯重量变化是产量下降的直接原因; 马铃薯整株和块茎的干物质积累均表现出明显的“S”型曲线特征, 连作缩短干物质快速增长期时间和减少干物质平均积累速度; 也影响马铃薯植株干物质在不同器官间的分配比例, 特别是明显增加根系干物质分配比例。在块茎产量的形成过程中, 长期连作导致营养器官花前贮藏干物质向块茎转运的贡献增加, 而花后同化产物向块茎直接输入的贡献降低。长期连作条件下马铃薯植株库源关系的失衡是导致块茎产量降低的主要原因。