部分引进猪种α1-岩藻糖转移酶基因多态性研究

本研究采用PCR RFLP技术对4个引进猪种杜洛克、约克夏、长白猪和皮特兰共计122头猪的FUT1基因进行了多态性分析。结果表明：4个猪种在该FUT1基因座位存在多态性，但抗性基因型AA仅在杜洛克和皮特兰猪中分布。卡方检验结果表明，长白猪与皮特兰间FUT1基因基因型分布差异极显著，长白猪与杜洛克猪之间差异显著，约克夏与皮特兰间差异也显著。     

不同猪种ESR基因多态性的PCR-RFLP分析

运用PCR-RFLP方法,检测了杜洛克、约克夏、长白猪、皮特兰、梅山猪、二花脸猪、枫泾猪、荣昌猪、苏太猪等9个国内外猪种的ESR基因PvuⅡ位点多态性.结果表明,在约克夏猪、二花脸猪、枫泾猪和苏太猪中检测到了AA、AB、BB 3种基因型,在杜洛克猪、皮特兰猪中检测到AB、BB 2种基因型,在长白猪、梅山猪、荣昌猪中只检测到了AB基因型.在本研究中不同品种的A、B基因频率差异显著,总体上在国外猪群体中A等位基因为优势等位基因,而地方猪种中B等位基因为优势等位基因.     

初夏季节二花脸与长白及约克夏公猪的行为性体温调节反应

四、五月份,观察二花脸猪、长白猪、约克夏猪的行为性体温调节反应,以探求其耐热性和体温调节特性.直肠温度与环境温度及体感温度均呈正相关随之升高而上升,且二花脸猪的直肠温度偏低,二花脸猪静立时间为19.4±12.4 min/h显著地长于长白和约克夏猪(P《0.05).     

德国的猪种改良及其繁育体系

根据德国养猪生产者工作协会统计,目前德国所养的猪当中,德国长白猪占68.4%,比利时长白猪为8.2%,皮特兰占19.1%,德国约克夏为3.5%。由此可见,德国长白猪已超过所有养猪量的三分之二,但它在最近十年来已经有所下降,同样地比利时长白猪的数量也在不断地减少。近年增长最快的是皮特兰,当然德国约克夏也有较大的发展。其主要原因是德国约克夏公猪与长白母猪的杂交不断增加,以进一步为生产仔猪而提供杂种母猪。随着杂交猪的迅速增加,在     

用RAPD标记研究7个猪种间的亲缘关系

试验利用随机扩增多态ＤＮＡ技术对互助猪、荣昌猪、甘肃白猪、长白猪、约克夏猪、杜洛克猪、汉普猪共７个品种的ＤＮＡ池进行了多态型研究,分析了各品种间的亲缘关系。结果显示：甘肃白猪虽然是由国外品种和地方品种培育而成,但其亲缘关系更接近于中国猪品种;约克夏猪与其它欧洲猪种亲缘关系较远,这可能与约克夏猪在培养过程中亲本遗传背景较为复杂有关。     

利用杂交手段提高猪肉质量

为了追求更好的食品质量目标,丹麦肉类研究所和Aarhus大学与丹麦猪研究中心一起成立了一个项目.项目研究小组打算生产5个不同的杂交猪种:杜洛克母猪和长白猪×约克夏母猪与卷毛公猪(匈牙利羊毛猪)和黑色伊比利公猪进行杂交(图1、2、3、4).除这4个杂交猪种之外,还设有一个对照猪种,即用长白猪×约克夏母猪和杜洛克公猪杂交生产出传统的屠宰猪.所有的猪在83 kg热胴体重时进行屠宰,表1列出了各杂交猪种的名称缩写和相应的屠宰日龄.     

五日龄窝活仔数对窝断奶仔猪数及仔猪成活率的影响

1992年以来,通过对丹麦长白猪及约克夏猪的总产仔数进行选育,使这一性状得到了显著的增长,但同时仔猪死亡率也大大增加。本研究的目标是估算出窝产仔数及成活率遗传和表型参数来寻找一种新的选育标准以改善窝断奶仔猪数。研究共收集了9300窝长白猪及6861窝约克夏猪的数据并利用了基于线性模型的REML进行分析,包括了母猪和与配公猪的遗传效应。结果表明,5日龄窝活仔数（N5D）的选育可以作为改善丹麦长白猪和丹麦约克夏猪的窝断奶仔猪数及仔猪成活率替代选育标准。     

初夏季节二花脸与长白及约克夏公猪的行为性体温调节反应

四、五月份,观察二花脸猪、长白猪、约克夏猪的行为性体温调节反应,以探求其耐热性和体温调节特性。直肠温度与环境温度及体感温度均呈正相关随之升高而上升,且二花脸猪的直肠温度偏低,二花脸猪静立时间为19.4±12.4min/h显著地长于长白和约克夏猪(P<0.05)。     

黑龙江省选育成功纯繁高产猪种

黑龙江省畜牧研究所的科研人员最近成功选育出4个世代的纯繁高产长白猪、约克夏猪，专家鉴定后认为，这项成果达到了国内同类研究的先进水平。 据了解，这项研究花费了5年时间，科研人员应用近代遗传育种理论，对长白猪、约克夏猪实行早期选种，加快世代遗传改进量速度，提高仔猪成活率。经检测，四世代长白猪、约克夏猪育肥期日增重分别为760g、818g，胴体瘦肉率分别达到63．7％和64．4％，仔猪成活率达到95％，提高了10％。这家研究所科研办的张兆良说，因选育成功的猪种体重增加，仔猪成活率高，增加了养猪户的收益，平均每头仔猪每1kg可…     

猪PLSCR4基因外显子区PCR-RFLP遗传多态性分析

本研究利用PCR-RFLP技术对我国6个地方猪种、中国野猪以及杜洛克、约克夏和长白3个国外猪品种,共307头猪的磷脂爬行酶基因(PLSCRs)外显子区的遗传变异进行了研究。结果表明:PLSCR4第7外显子处存在T68C的同义突变,第8外显子处存在G4A的错义突变;在T68C突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪6个猪群优势等位基因为T,荣昌和野猪群体中优势等位基因为C;在G4A突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪群体中优势等位基因为G,藏猪、荣昌、金华及野猪群体中优势等位基因A。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.