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张薇 《中国农业信息快讯》2014,(4S):273-274
解析"场中校"生产性实训基地的内涵,以湖北生物科技职业学院省级战略新兴(支柱)产业人才培养专业种子生产与经营专业省级实训基地建设为例,探索涉农专业"场中校"生产性实训基地的建设思路和建设方法。 相似文献
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园林专业校内生产性实训基地建设探索与实践——以徐州生物工程职业技术学院为例 总被引:1,自引:0,他引:1
分析了徐州生物工程职业技术学院校内园林实训基地建设中存在的问题,提出了园林专业校内生产性实训基地建设、运行的策略和措施。 相似文献
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在高职院校市场营销专业校内实训基地建设中,生产性实训基地是重要的建设内容,如何结合市场营销专业的特征,分析目前高职院校市场营销专业校内实训基地建设现状,运用"教学工厂"理念加强"教、学、做"一体化,值得探讨。 相似文献
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根据人才能力培养需求及实训基地顺畅管理与运行的需求,结合畜牧兽医专业特点,打破常规思路,突破性地将生产性实训基地分类细化为校企合作经营型、企业独立经营型、学校自主经营型、科技服务研发型和学生自主经营型5个类型,创新了基地构建和管理路径,在解决传统教学实训基地问题的基础上,为农业高职畜牧兽医类生产性实训基地的构建和运行提供了解决思路和实践示范。 相似文献
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在对高职环境监测与治理技术职业技能大赛和当前职业院校师生知识技能情况进行分析的基础上,通过创新技能大赛学生选拔与培养机制,革除大赛精英化的弊端,让全体学生受益。基于职业技能大赛,将大赛有机地融入到专业教育教学改革中,不断探索环境监测与治理技术专业人才培养新模式,改革人才培养方案,提高教师技能水平。以环境监测与治理技术专业为例,提出如何真正让技能大赛发挥引领作用,推进高职院校专业综合改革。 相似文献
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Oberstrass FC Auweter SD Erat M Hargous Y Henning A Wenter P Reymond L Amir-Ahmady B Pitsch S Black DL Allain FH 《Science (New York, N.Y.)》2005,309(5743):2054-2057
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor. 相似文献
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The Permian-Triassic boundary (PTB) event, which occurred about 251.4 million years ago, is marked by the most severe mass extinction in the geologic record. Recent studies of some PTB sites indicate that the extinctions occurred very abruptly, consistent with a catastrophic, possibly extraterrestrial, cause. Fullerenes (C60 to C200) from sediments at the PTB contain trapped helium and argon with isotope ratios similar to the planetary component of carbonaceous chondrites. These data imply that an impact event (asteroidal or cometary) accompanied the extinction, as was the case for the Cretaceous-Tertiary extinction event about 65 million years ago. 相似文献
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Hardy WR Li L Wang Z Sedy J Fawcett J Frank E Kucera J Pawson T 《Science (New York, N.Y.)》2007,317(5835):251-256
Changes in protein-protein interactions may allow polypeptides to perform unexpected regulatory functions. Mammalian ShcA docking proteins have amino-terminal phosphotyrosine (pTyr) binding (PTB) and carboxyl-terminal Src homology 2 (SH2) domains, which recognize specific pTyr sites on activated receptors, and a central region with two phosphorylated tyrosine-X-asparagine (pYXN) motifs (where X represents any amino acid) that each bind the growth factor receptor-bound protein 2 (Grb2) adaptor. Phylogenetic analysis indicates that ShcA may signal through both pYXN-dependent and -independent pathways. We show that, in mice, cardiomyocyte-expressed ShcA directs mid-gestational heart development by a PTB-dependent mechanism that does not require the pYXN motifs. In contrast, the pYXN motifs are required with PTB and SH2 domains in the same ShcA molecule for the formation of muscle spindles, skeletal muscle sensory organs that regulate motor behavior. Thus, combinatorial differences in ShcA docking interactions may yield multiple signaling mechanisms to support diversity in tissue morphogenesis. 相似文献
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研究采用RT-PCR技术结合克隆测序方法,克隆出全长2 004 bp NUMB基因序列,其中包括1 782 bp开放读码框,该基因编码592个氨基酸。利用生物信息学分析软件对该基因氨基酸序列预测与分析发现:NUMB蛋白二级结构中,转角占很大比例,且蛋白亲水性较强,结构功能域分析发现该蛋白多肽链存在一个N端磷酸酪氨酸结构域(PTB),位于第37~162氨基酸之间。基于不同物种NUMB蛋白序列所构建分子进化树表明,猪与牛、骆驼亲缘关系最近,而与斑马鱼最远。在m RNA水平上,NUMB基因肺中表达量最高,大肠中表达量最低。 相似文献
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Garcia CK Wilund K Arca M Zuliani G Fellin R Maioli M Calandra S Bertolini S Cossu F Grishin N Barnes R Cohen JC Hobbs HH 《Science (New York, N.Y.)》2001,292(5520):1394-1398
Atherogenic low density lipoproteins are cleared from the circulation by hepatic low density lipoprotein receptors (LDLR). Two inherited forms of hypercholesterolemia result from loss of LDLR activity: autosomal dominant familial hypercholesterolemia (FH), caused by mutations in the LDLR gene, and autosomal recessive hypercholesterolemia (ARH), of unknown etiology. Here we map the ARH locus to an approximately 1-centimorgan interval on chromosome 1p35 and identify six mutations in a gene encoding a putative adaptor protein (ARH). ARH contains a phosphotyrosine binding (PTB) domain, which in other proteins binds NPXY motifs in the cytoplasmic tails of cell-surface receptors, including the LDLR. ARH appears to have a tissue-specific role in LDLR function, as it is required in liver but not in fibroblasts. 相似文献
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茶尺蠖核型多角体病毒(Ectropis obliqua nucleopolyhedrosis virus,EoNPV)是一种重要的病原微生物,为进一步明确该病毒对茶尺蠖两近缘种的毒力差异,采用叶盘法对3龄茶尺蠖和灰茶尺蠖幼虫进行了EoNPV的毒力测定。研究显示,EoNPV对灰茶尺蠖的半致死剂量LD50(median lethal dose)为茶尺蠖的28.9倍,EoNPV对灰茶尺蠖的致死中时间LT50(median lethal time)大于茶尺蠖。结果表明,EoNPV对茶尺蠖两近缘种间的毒力存在差异,EoNPV对茶尺蠖具有更高的致病力,研究结果对EoNPV田间防治茶尺蠖两近缘种具有现实指导意义。 相似文献
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薰衣草茎段再生体系的建立 总被引:2,自引:1,他引:1
以薰衣草带芽茎段为外植体,探讨不同激素种类与水平等因素对其愈伤诱导、不定芽分化、增殖与不定根形成的影响,并筛选了薰衣草试管苗过渡移栽的适宜基质等。试验结果表明:不定芽启动培养以MS+BA1.0~2.0mg/L+蔗糖30g/L+琼脂6.5g/L为宜;继代增殖以MS+BA1.0~2.0mg/L+IBA0.25mg/L+GA1.0mg/L+蔗糖30g/L+琼脂6.5g/L为宜;不定根分化以White+IBA0.4mg/L+蔗糖20g/L+琼脂6.5g/L或White+NAA0.8mg/L+蔗糖20g/L+琼脂6.5 g/L为宜。 相似文献
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【目的】建立库尔勒香梨叶片诱导再生的稳定高效再生体系。为梨的遗传转化研究奠定基础。【方法】以库尔勒香梨离体培养的继代30 d左右的无菌苗叶片为材料,1/2 MS为基本培养基,用 TDZ与 IBA或 NAA分别组合设计配方,研究不同生长调节剂质量浓度组合对不定芽诱导过程中愈伤组织形成率和不定芽形成率的影响,选取最优配方附加不同质量浓度的AgNO3,获得不定芽再生的最佳培养基。用IBA和NAA分别组合设计生根生长调节剂的配比,根据生根率、平均生根数以及平均生根长度筛选最适宜生根的生长调节剂的配比,建立生根的培养体系。【结果】库尔勒香梨叶片诱导不定芽再生的最佳培养基为1/2 MS+TDZ 1.0 mg/L+IBA 0.5 mg/L+AgNO3 0.5 mg/L,愈伤组织诱导率100%,不定芽形成率84.92%。最适宜香梨再生苗生根的培养配方为1/2 MS + 0.5 mg/L IBA + 0.9 mg/L NAA,结合前期暗培养7 d,生根率可达100%,平均生根条数为5.66,平均根长度为 2.8 cm。【结论】建立了库尔勒香梨的再生体系。 相似文献