庐山山地垂直自然带土壤腐殖质与土壤动物分布特征

沿庐山垂直自然带谱对常绿阔叶林(低海拔,L)、落叶常绿混交林(中海拔,M)和落叶阔叶林(高海拔,H)地面腐殖质及大中型土壤动物分布情况进行调查分析,使用调查数据探究了该垂直自然带土壤腐殖质与土壤动物分布特征。调查分析的腐殖质指标有剖面各发生层厚度、O-A过渡层厚度以及A层土壤团粒大小和有机质、总碳量、全磷、全氮、全碳含量和pH值等,土壤动物指标有类群数、个体密度和多样性指数。结果表明,L、M、H自然带地面腐殖质分别以细腐殖质(Mull)、半腐殖质(Moder)和粗腐殖质(Mor)为主;调查共获得隶属于3门8纲24目的大中型土壤动物2636头,其平均密度为2245.23 ind m^-2,优势类群前气门亚目、甲螨亚目、弹尾目动物个体数分别占总个体数的38.87%、25.36%和13.37%。皮尔逊相关性分析和典范对应分析(CCA)结果表明,土壤动物个体密度与A层土壤养分含量、类群数与O层厚度关系密切,而多样性指数既与A层土壤pH值、也与A层土壤养分含量及O-A过渡层厚度密切相关。土壤动物分布总体特点是优势类群在不同腐殖质组型土层广泛分布,而常见类群和稀有类群则分别与腐...     

我国中部井工煤炭开采沉陷对农田大型土壤动物群落结构的影响

为了解不同沉陷程度农田土壤生态系统中大型土壤动物群落结构,于2007年夏季对我国中部潞安集团井工开采区沉陷农田生态系统中不同等高线处大型土壤动物群落组成进行了调查,采用野外手捡法共采集大型土壤动物477只,隶属于3门7纲15目22科。其中,蜘蛛目、鞘翅目、直翅目和膜翅目为优势类群,占总捕量的83.64%;常见类群4类,占总捕量的13.22%。研究结果表明:不同沉陷程度对农田大型土壤动物个体数、类群数均有不同程度影响,农田大型土壤动物个体密度随着沉陷深度的增加呈现增加趋势,沉陷区土壤生态环境的变化引起大型土壤动物的局部迁移。Jaccard相似性系数表明沉陷区中部(2 m、4 m和6 m等高线处)三者间相似程度较高,正常农田(CK)与沉陷区底部(7 m等高线处)之间相似程度最低,达到极不相似程度。研究认为不同沉陷程度对大型土壤动物的群落结构有明显影响,将大型土壤动物作为沉陷区农田土壤质量评价中生物性状指标具有重要意义。     

Distribution pattern of total lipids and lipid fractions in forest humus

Mull, moder, and mor humus profiles were investigated for their total lipid contents and the behaviour of lipid-phosphorus and of five neutral lipid classes identified by HPTLC. The quantitative distribution of total and neutral lipids among the humus types is, besides by natural differences between the various parent litter materials, mainly controlled by soil physicochemical properties. In particular strongly acidic conditions, i.e. conditions corresponding to a reduced biological activity, inhibit significantly the decomposition of lipid compounds in (moder and) mor humus. A feature of the lipid depth functions is the input of microbial and root litter-derived lipid material in the fermentation layers and a considerable lipid accumulation in the A horizons, probably at least partly due to mechanical transport processes. Phospholipids show no distinct variation dependent on the humus type, as far as the organic layers are concerned. But obviously, lipid-P in the mineral, soil is closely correlated with, and therefore is a valuable measure for, the overall biological activity increasing from mor to moder and mull humus.     

长白山地丘陵区不同土地利用方式土壤动物群落生态分布特征

在长白山地丘陵区选择次生落叶阔叶林、灌木林、采伐迹地以及耕地四种土地利用方式,对其土壤动物群落的组成、多样性和分布特征进行研究。结果表明:不同土地利用方式土壤动物群落的水平分布呈现明显的差异。采伐迹地中型土壤动物个体密度和耕地中型土壤动物类群数季节变化明显。次生落叶阔叶林和灌木林土壤动物分布随土层加深急剧减少,采伐迹地和耕地垂直分布递减和缓。同一季节土壤动物的丰富度指数和多样性指数在不同土地利用方式差异显著。土壤动物的多样性表现为灌木林最高,耕地最低。不同土地利用方式影响土壤动物群落的生态分布,其中pH、土壤有机质、有效磷和速效氮是影响长白山地丘陵区不同土地利用方式土壤动物群落分布的主导因子。     

Leaching of n and c from birch leaf litter and raw humus with special emphasis on the influence of soil fauna

To examine the role of a community of soil animals in N-mineralization and C fluxes in dead organic matter, we established a microcosm system with substrates composed of: (a) birch leaf litter; (b) raw coniferous humus; and (c) litter on humus. Every 3–4 wks the substrates were irrigated with distilled water, and the amounts of NO₅-N, NH₄-N, total-N and total-C (as well as humic substances at one recording) in the leachates were analyzed. At the end of the experiment, water-soluble and exchangeable forms of N were measured in the test materials.

The differences in the release of N were clear both between the replicates with and without soil animals and between the different forms of N. From wk 10 onwards the fauna enhanced significantly the leaching of total-N and NH₄-N from the humus alone. The release of total-N from the litter alone was also increased by the fauna. The presence of animals did not significantly affect any form of K.CIextractable N.

The amounts of total C in the leachates increased significantly in almost all samples when the fauna was involved. In addition, the fauna affected the humification process positively in the litter + humus and in humus alone, i.e. relatively more humic substances than total C were liberated from the materials in the presence of animals.     

Microbial dynamics in Mediterranean Moder humus

There is a growing interest in the links between humus forms and soil biota, and little is known about these links in Mediterranean ecosystems. Culture-independent techniques, such as DNA extraction followed by DGGE and enzyme activities, allowed us to compare microbial communities in two horizons of a forest soil in different seasonal conditions. Direct in situ lysis was applied for extraction of DNA from soil; intracellular DNA was separated from extracellular and used to represent the composition of microflora. The aims were to describe how biochemical and microbiological parameters correlate with topsoil properties in typical Mediterranean Moder humus. Changes in bacterial and fungal community composition were evident from DGGE profiles. Degrees of similarity and clustering correlation coefficients showed that the seasonal conditions may affect the composition and activity of bacterial and fungal communities in the OH horizon, while in the E horizon the two communities were hardly modified. In the same season, OH and E horizons showed a different composition of bacterial and fungal communities and different enzyme activities, suggesting similar behaviour of eubacteria and fungi relatively to all the variables analysed. Evidently, different organic carbon content in soil horizons influenced microflora composition and microbial activities involved in the P and N cycles.     

科尔沁沙地土地利用变化对大型土壤节肢动物群落影响

土地利用类型是地球表层系统最突出的景观标志,不仅可以客观地展示地球表面特征空间格局的活动,还反映着地球表面景观的时空动态过程[1-2].由于土地利用变化与人类活动和全球气候变化密切相关,对生物多样性消长、生态环境演变、生态安全水平以及人类可持续性发展有着重要影响,因而其研究已成为当今全球变化研究的前沿和热点课题[1,3].     

Morphological characterisation of humus forms in recent coastal dune ecosystems in Belgium and northern France

The morphology of humus profiles, developed on recent sandy coastal dune ecosystems, is studied on macro- and microscale. Humus and soil profiles were described, sampled and analysed. Undisturbed, oriented samples were taken from the surface horizons for micromorphological study. The soils are situated in the National Biological Reserve of the Opal Coast, Merlimont, France and near De Haan, Belgium. On both sites, the parent material consists of aeolian calcareous sand. Surface horizons, however, are decarbonated due to leaching processes.In Merlimont, the sequence consists of two profiles, one under Pinus nigra ssp. laricio and one under Populus nigra ssp. nigra, characterised by a Resimor¹/Dysmoder^{, 2} and Rhizomull^{, 1}/Mesomull ^{, 2} humus type, respectively. At the De Haan site, three profiles under Quercus robur, Populus ssp. and P. nigra var. austriaca are investigated. The humus types are respectively Mullmoder^{, 1} to Mull/Dysmull ^{, 2}, Mull ^{, 1}/ Oligomull ^{, 2}, Mormoder ^{, 1}/Dysmoder ^{, 2}.Differences among the humus profiles are related to presence/absence among the L-, F-, H- and A-horizons, structure, rooting, presence of hyphae, sclerotia and excrements.Macromorphologically, the difference between humus profiles under coniferous and deciduous forest/grassland is evident by thicker L-, F and/or H-horizons under coniferous trees. The microstructure of the L- and F-horizons shows a loose packing of subhorizontally oriented needles. The microstructure of the H-horizons varies from intergrain microaggregate to locally weak crumb. The microstructure of the surface mineral horizon of all profiles is mainly intergrain microaggregate, sometimes single grain and exceptionally bridged grain. Intimate mixing and complexation of organic matter to the mineral fraction into aggregates is very limited in the A-horizons. All A-horizons, irrespective of the humus type, are to some degree characterised by a “pepper and salt” appearance. The horizon symbol “H+E” is introduced to label this morphology.The Mull humus forms under deciduous trees are indicative of rather intense mesofauna activities. The absence of a crumb structure in the A-horizon of these soils could be due to the fast disintegration of earthworm casts in these sandy soils with no clay or silt fraction.     

Humus forms,organic matter stocks and carbon fractions in forest soils of northwestern Italy

Humus forms may be the first tool to assess qualitatively organic matter turnover in soils; as such they should be related to the stocks of organic C a soil can store, to the characteristics of organic matter that affect its stability and, more generally, to the factors of soil formation. In this work, we tested these hypotheses in 27 forest soils of northwestern Italy. Site variables representing the pedogenic factors allowed classifying the plots into three clusters, which were significantly different for soil and humus types. The average stocks of organic C in the humic episolum (organic and top mineral horizons) ranged from 2.7 kg m⁻² in Eumulls to 9.5 kg m⁻² in Amphimulls. A clear trend in C stocks was visible and related both to the increasing presence of organic layers where the environmental conditions do not favour a rapid turnover of organic matter and to the good mixing of organics and minerals in “bio-macrostructured” A horizons. The characteristics of organic matter were also linked to humus forms: The proportion of humified complex substances was the highest in the most active forms, and conversely, non-humified extracted substances formed a considerable part of organic matter only where the environmental conditions limit organic matter degradation. Humus forms seem therefore to reflect several mechanisms of organic matter stabilisation and are clearly related to the capacity of the soil to store C.     

Humus Forms in Forest Soils: Concepts and Classifications

The concepts and classifications of humus forms developed since the time of scientific pedology formation are critically discussed. The concept of humus forms (types) relates to the classification of a set of topsoil organic and organomineral horizons, which reflects morphologically distinct phases of plant litter and soil organic matter decomposition, but not to the fractions of soil organic matter. Humus forms reflect various types of transformation and accumulation of organic matter in the soil. The stages of development and modern classifications of humus forms abroad are described. The taxonomy of humus forms in Russian literature and its application for the mapping and evaluation of forest soils are considered, as well as its use for the mathematical simulation of soil organic matter mineralization and humification. Prospects for the development of the classification of humus forms in combination with the basic soil classification of European Russia are discussed. A call for an understanding and a common language in soil science at the international level is underlined.     

土壤动物在土壤有机质形成中的作用

作为土壤生态系统重要组成部分的土壤动物,在土壤元素循环转化和迁移过程中发挥着重要的作用。土壤动物是凋落物分解的"微型粉碎机",通过体内"特殊转换器",影响土壤有机质的转化、腐殖质的形成。本文从土壤动物对地表枯落物分解入手,分析了影响土壤动物对凋落物分解的因素,土壤动物通过刺激土壤酶活性,与土壤微生物群落一起,加快土壤有机物的分解,促进土壤腐殖质的转化。旨在拓宽土壤动物生态功能,丰富土壤腐殖质形成机理学说,对保护土壤生物多样性、提高土壤地力、保障粮食安全具有重要的科学意义。     

Soil animal communities in holm oak forests: influence of horizon, altitude and year

Soil animals (macro and microarthropods, annelids, nematodes) were sampled along an altitudinal gradient and over 2 years in holm oak (Quercus rotundifolia) forests of the Moroccan Atlas. We studied the influence of elevation and year on the vertical distribution of soil fauna. Whatever the elevation (1500, 1700 and 1900 m), the humus form was a Dysmull, with a thick litter horizon and a fine crumb A horizon. Thirty-six categories of fauna were found and classified at the group level. The influence of horizon, altitude and year was analysed by analyses of variance (ANOVA, on seven broad zoological groups and on total fauna) and correspondence analysis (on 36 zoological groups). There was a decrease in the population size of most zoological groups from organic (OL, OF) to mineral horizons (A, S), but OL and OF horizons varied as the most populated horizon according to years and animal groups. More animals and more animal groups were present at higher elevation, following an increase in food and habitat availability.     

大兴安岭不同冻土带土壤动物生态地理研究

2003年6月、8月、10月对大兴安岭不同冻土带选择沼泽、柳丛、耕地、次生林群落进行土壤动物调查，共获土壤动物39225只，隶属于4门8纲24目。由对大型、中小型及湿生三类土壤动物的数量分析可知，三类土壤动物数量的变化在群落间并不总是成正相关的关系。水平分布调查表明，中小型土壤动物的个体数量和类群数量从岛状冻土带到连续多年冻土带逐渐减少，该分布与土壤动物分布的一般规律（土壤动物分布通常符合纬度地带性分布规律，即由低纬向高纬逐渐减少）一致，但大型土壤动物的个体数量、类群数和生物量基本上从岛状冻土带到连续多年冻土带逐渐增加（即由低纬向高纬逐渐增加），湿生动物的数量也如此分布，这与土壤动物分布的一般规律不一致甚至相悖，这一特点表现出生态系统局部-区域相互关系的复杂性；土壤动物的类群数、个体数量与土壤有机质、全氮的含量呈明显的正相关；垂直分布调查表明，中小型土壤动物的个体数量和类群数量分布表现出明显的表聚性，但大型土壤动物的个体数量与生物量最大值出现在第二层；土壤动物多样性分析表明，中小型土壤动物的多样性指数从岛状冻土带到连续多年冻土带逐渐减小，而大型土壤动物的多样性指数在不同冻土带间的变化规律不明显。     

Effects of air drying on the respiration of forest soil microbial populations

Microbial populations from dried, remoistened and undried forest organic horizons were allowed to thoroughly recolonize sterilized forest organic horizons; oxygen uptake was followed during recolonization. Microbial damage due to air drying was in the order litter (L) > fermentation (F) > humus (H). Species diversity, as indicated by diminished O₂ uptake, was reduced in all horizons which had been air dried, with greatest damage in the litter horizon. Samples of these forest organic soil materials, designated for the study of microbially driven processes, should not be air dried.     

Effect of Contrasting Trophic Conditions on the Priming Effect in Gray Forest Soils

Priming effects initiated by the addition of ¹⁴С glucose have been compared for humus horizons of soils existing under continuous input of fresh organic substrates and for buried soil horizons, in which entering of organic matter has been essentially limited. The effect of microrelief on the manifestation of priming effect in the humus horizons of gray forest soil on microhigh and in microlow has been estimated. Humus horizon in soils on microhigh, not activated by glucose, produced two times more СО₂ in comparison with soils of microlow. However, the introduction of glucose canceled the effect of microrelief on СО₂ emission. The intensity of absolute priming effect correlated with the С_org pool, initial microbial biomass, and enzyme activity, decreasing from humus horizons to the buried ones, and did not depend on microrelief. The effect of microrelief was observed, when assessing the priming effect relative to control (soil not activated by glucose): the value of relative priming effect was 1.5 times greater in А horizon of gray forest soil in microlow in comparison with that on microhigh being the result of increasing activity of enzymes.     

Mycological complexes in Holocene and Late Pleistocene paleohorizons and in fragments of paleosols

Fragments of buried Late Pleistocene (30000-year-old) and Early Holocene (10000-year-old) paleosols contained viable complexes of microscopic fungi. The mycobiota of these paleosols represents a pool of fungal spores that is lower in number and species diversity as compared to that in the recent humus horizons and higher than that in the inclosing layers. The central part of the paleosol profiles is greatly enriched in microscopic fungi. In the intact humus horizons of the Late Holocene (1000–1200 years) paleosols, actively functioning fungal complexes are present. These horizons are characterized by their higher level of CO₂ emission. The buried horizons, as compared to the recent mineral ones, contain a greater fungal biomass (by several times) and have a higher species diversity of microscopic fungi (including fungi that are not isolated from the recent horizons). Nonsporulating forms are also present there as sterile mycelium. The seasonal dynamics of the species composition and biomass of the fungal complexes were more prominent and differed from those inherent to the surface soil horizons. In the buried humus horizons, the dynamics of the fungal biomass were mainly due to the changes in the content of spores. The data on the composition of the fungal complexes in the buried soils confirm (due to the presence of stenotopic species) the results of paleobotanic analyses of the past phytocenoses or do not contradict them.     

Origin of the nitrogen assimilated by soil fauna living in decomposing beech litter

We investigated the nitrogen source for main taxa of soil fauna in two beech forests of contrasted humus type using ¹⁵N-labelled beech litter and ¹⁵N analysis of soil fauna. ¹⁵N-labelled beech litter was deposited on the topsoil in December 2000 in four stands of different ages at Leinefelde (Germany) with mull humus and in one mature stand at Sorø (Denmark) with moder humus. The fate of the tracer isotope was measured in litter and soil, as well as in the soil fauna, and for each taxa, we calculated the proportion of N in the animal derived from the labelled substrate. Of the original N contained in the litter, 20-41% was lost after 9 months at Leinefelde, and only 10% at Sorø. This loss was counterbalanced by the incorporation of 24-31% external N at Leinefelde, and 31% at Sorø, partly originating from fungal colonisation of the added litter. The proportion of N assimilated from the labelled litter by the different soil animals varied in relation to their mobility and feeding preferences. Large and mobile soil animals, especially predators, derived on average less ¹⁵N because they were also able to feed outside the labelled litter boxes. Detritivores assimilated at most 15% of their nitrogen content at Leinefelde and 11% at Sorø from the decomposing labelled litter. The most labelled taxa at Leinefelde were small fungivorous and coprophagous species, mainly isotomid Collembola such as Isotomiella and Folsomia. At Sorø, best labelled taxa were saprophagous species such as Enchytraeidae, Glomeridae and Phthiracaroidea. These low rates of ¹⁵N assimilation indicate that fresh litter is not directly the main N source for soil animals. The results obtained suggest that soil fauna fed preferentially upon microorganisms colonising the litter at Leinefelde (mull) and from litter itself at Sorø (moder).     

Effects of soil fauna on leaching of nitrogen and phosphorus from experimental systems simulating coniferous forest floor

Summary Long-term experiments (97–98 weeks) were carried out in macrocosm systems simulating the complexity of coniferous forest soil. The macrocosms were partially sterilized by freezing, thawing and drying, then re-inoculated with microbes alone or microbes + soil fauna. Removable microcosms containing birch litter, spruce litter, or humus were inserted into the substrate humus in the macrocosms. Two experiments used organic matter only, and in the third there was mineral soil below the humus. The macrocosms were incubated in climate chambers that simulated both summer and winter conditions. At 4- to 6-week intervals the substrates were irrigated for analyses of pH, total N, NH ₄ ⁺ –N, NO ₃ ^– –N, and PO ₄ ^3– –P in the leachates. At the end of each growing season a destructive sampling was performed, including analyses of KCl-extractable N and P.Leaching of NH ₄ ⁺ and PO ₄ ^3– from both the litter and the total systems was significantly enhanced by the soil fauna. There were also differences in mineralization of N and P between the refaunated systems, apparently due to divergent development of the faunal communities. In general, fauna affected KCl-extractable nutrients from the litter positively, although this effect was less evident than in the leaching water. In the humus and mineral soil the fauna significantly increased the release of N and P, especially in the later stages of the experiments. Soil pH was higher in the presence of fauna, but there was no difference in the pH of the leachates. Not only invertebrate-microbial interactions, but also mutual relationships among fauna were important in the nutrient dynamics.     

Distribution of Zinc,Manganese, Copper,Cobalt, and Nickel in Andosols Profiles

The distribution of zinc, manganese, copper, cobalt, and nickel in Andosols was investigated. Sixty nine soil samples were collected from different horizons of an Andosols profile in Miyakonojo Basin in south Kyushu, Japan, The total contents of heavy metals were determined by digestion and four extraction solutions, 1 M NH₄Ac (ammonium acetate) pH 4.5, 0.1 M HCl, 0.01 M EDTA (ethylenediaminetetraacetic acid) pH 6.5, and 0.005 M DTPA (diethylenetri-aminepentaacetic acid) pH 7.3 were used to determine the contents of available Zn, Mn, Cu, Co, and Ni in Andosols in relation to the organic carbon content. The results of the extraction analysis showed that by the use of 0.1 M H Cl high value of extracted heavy metals in the upper layers of the humus horizons were obtained while EDTA extraction yielded a large amount of the above mentioned metals in the high humus horizons. The extractable heavy metals contents were high and these metals closely related to the organic carbon content mostly in the humus horizons in the profile. Where, biocycling process may play an important role in the concentration of heavy metals. Based on the study, it was found that the total content of Zn increased towards the C horizons or pumice layers in the soil profile. Such a trend was also found in the case of the Mn content. While the Cu content in the humus horizons was much higher in the upper part of each humus horizon. According to this study the distribution of heavy metals, Cu (organic matter complexes) in the Andosols profile was more stable than that of Zn (organic matter complexes) in soils. It was shown that Zn in the surface humus horizon was enriched but that some amount was leached under buried conditions. The same phenomenon was also observed in the distribution of Mn in the profile. The movement of Co and Ni in the soil profile was limited, as evidenced by the sharp reduction in the concentrations of these two metals in buried soils.

Hence, it is concluded that the distribution of Zn, Mn, Cu, Co, and Ni was considerably higher in the humus horizons of the Andosols profiles.     

A microcosm study on the respiration and weight loss in birch litter and raw humus as influenced by soil fauna

Summary The effect of diverse soil fauna (Collembola, Acari, Enchytraeidae, Nematoda) on decomposition of dead organic matter was studied in microcosms containing (1) birch leaf litter, (2) raw humus of coniferous forest and (3) litter on humus. Total respiration (CO₂ evolution) was monitored weekly, and mass loss, length of fungal hyphae (total and metabolically active) and survival of animal populations were checked at the end of weeks 12 and 21–22 from the start of experiment. Animal populations established themselves well during the incubation. At the end of the experiment some replicates containing litter had microarthropod densities of up to 500 specimens per microcosm, corresponding to a field population of 200 000 m^–2. The soil animals had a positive influence on total respiration in all substrates. By the end of experiment 32.0%, 22.6% and 14.6% more CO₂ had evolved in the presence of animals in litter, litter + humus and humus alone, respectively. There was clear trend towards a higher mass loss in the presence of animals, though it was significant in litter only. Our results showed that a diverse soil animal community enhances the activity of soil microbes, and may thereby accelerate decomposition in raw coniferous forest soil.