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Abstract A programme for the biological control of the citrus leafminer Phyllocnistis citrella Stainton (Lepidoptera, Gracillariidae) was carried out in Tunisia between 1996 and 1998. Two exotic parasitoids, Ageniaspis citricola (Logvinovskaya) (Hymenoptera, Encyrtidae) and Semielacher petiolatus Girault (Hymenoptera, Eulophidae), were introduced from Australia, mass-reared in a greenhouse on Citrus aurantium L. seedlings infested with P. citrella, and released in different orchards located in the major citrus-growing area of Tunisia. In 1996, A. citricola was recovered in 39.13% of release sites, reaching a maximum percentage of parasitism of 28%. In 1997, the parasitoid was recovered in a third of all release points, with an average percentage of parasitism of 5%. However, the wasp was unable to survive the winter 1998, and has become extinct. The non-establishment of A. citricola may have been due to the more arid climatic conditions present during the rainless summer and most of the autumn in Tunisia. By contrast, the establishment, dispersal, and reduction in citrus leafminer population observed with S. petiolatus between 1997 and 1998 shows this species to be well-adapted to the Tunisian climate. The average percentage parasitism of P. citrella by S. petiolatus increased from 6.6% in 1997 to 15% in 1998. Moreover, S. petiolatus was established in 76% of release points in 1997 and in 82% in 1998. It spread as much as 30 km from some release points, with no apparent decline in parasitism levels with distance. Semielacher petiolatus is considered to be an effective biological control of the leafminer in Tunisia. Its biological control action would supplement the action of the native parasitoids Pnigalio sp. and Cirrospilus pictus Nees (Hymenoptera, Eulophidae). 相似文献
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Citrus farmers in the Mekong Delta have a long tradition of managing the weaver ant Oecophylla smaragdina (Fabricius). From 1994 to 1998, insecticide use increased significantly (P< 0.01) from 66% to 84% in orchards where O. smaragdina occurred. In 1998, ca 75% of the sweet orange (Citrus sinensis) and 25% of the Tieu mandarin (C. reticulata) orchards had large O. smaragdina populations, due to lower pesticide pressure in the first crop. In orchards with O. smaragdina, farmers sprayed less frequently and used fewer highly hazardous insecticides. Major insecticides used in sweet orange were monocrotophos and alpha-cypermethrin, and those used in Tieu mandarin were methidathion, imidacloprid and fenpropathrin. Expenditure on pesticides was reduced by half when O. smaragdina was abundant, without affecting either the yield or the farmers' income. Therefore, O. smaragdina husbandry is a good example of a traditional practice which should be further promoted as an important component of sustainable citrus production. The experience of those farmers who use no or few pesticides should be drawn upon in developing farmer training programmes or mass media tools to promote IPM in citrus. Farmers practicing ant husbandry were significantly older than those not doing so. 相似文献
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Compared with an unselected susceptible population, a cotton bollworm, Helicoverpa armigera (Hübner), population selected for 22 generations with transgenic cotton leaves (modified Cry1A) in the laboratory developed 11.0-fold resistance to Cry1Ac (one single-protein product MVPII). Resistance to Bacillus thuringiensis Berliner subsp kurstaki (Btk) was selected for 22 generations with a 5.2-fold increase in LC50. The estimated realized heritabilities (h2) of resistance for transgenic-cotton- and Btk-selected populations were 0.1008 and 0.2341, respectively. This reflects the higher phenotypic variation in response to Cry1Ac in the transgenic-cotton-selected population. This variation may have been caused by differences in protein toxin levels expressed in different growth stages of the transgenic cotton. Because of the different slopes of the probit regression lines between Cry1Ac and Btk, the estimated realized h2 cannot be used visually to compare resistance development to Cry1Ac and Btk in H armigera. Thus, the response quotient (Q) of resistance was also estimated. The Q values of resistance for transgenic-cotton- and Btk-selected populations were 0.0763 and 0.0836, respectively. This showed that the rate of resistance development would be similar in both selection populations. This result indicates that the selection of resistance using transgenic cotton is different from that selected using the single toxin. Resistance risk to transgenic cotton and Btk in field populations was assessed assuming different pressures of selection by using the estimated h2. Assuming the h2 of resistance in a field population was half of the estimated h2, and the population received prolonged and uniform exposure to transgenic cotton or Btk causing >70% mortality in each generation, we predicted that resistance would increase 10-fold after <23 generations for Cry1Ac in transgenic cotton-selected-populations and after <21 generations for Btk in Btk-selected populations. Cross-resistance would be expected after <48 generations for Btk in transgenic-cotton-selected populations and after <21 generations for Cry1Ac in Btk-selected population. The results show that the potential to evolve resistance is similar in both transgenic-cotton- and Btk-selected populations, but that cross-resistance development to Btk is slower in transgenic-cotton-selected populations than cross-resistance development to Cry1Ac in Btk-selected populations. 相似文献
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Abstract The cold-hardiness of Liriomyza chinensis pupae was measured in the laboratory by observing pupal mortality at low temperatures. Pupal mortality increased with decreasing temperature and with extended cold exposure time. No recently pupated pupae (6 h) were able to survive after 16 days of chilling at 0, 2.5 and 5°C, but 42.9% survival was observed at 10°C. Pupae at different developmental stages showed significant difference in mortality, with very low levels of mortality observed for older pupae (4 and 7 days) after exposure to 0°C for 16 days. The lethal time for 50% survival (LT50) increased with increasing age of pupae. LT50 for 4-day-old pupae exposed to 0°C was 52.1 days. Analysis of mean temperatures at several localities indicated that L. chinensis is able to overwinter outdoors in southern regions, but is unable to overwinter in open fields in northern regions of Japan, suggesting that overwintering in these regions would only occur in greenhouses. 相似文献
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Mohd Amir F Abdullah Saad Moussa Milton D Taylor Michael J Adang 《Pest management science》2009,65(10):1097-1103
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Huang F Ghimire MN Leonard BR Wang J Daves C Levy R Cook D Head GP Yang Y Temple J Ferguson R 《Pest management science》2011,67(10):1269-1276
BACKGROUND: In the mid‐southern region of the United States, sugarcane borer, Diatraea saccharalis (F.), is a major target pest of transgenic maize expressing Bacillus thuringiensis (Bt) proteins. Novel transgenic maize technologies containing two or more pyramided Bt genes for controlling lepidopteran pests have recently become commercially available. Insect resistance management (IRM) is an important issue in the sustainable use of Bt crop technologies. The objective of this study was to determine the frequency of resistance alleles in field populations of D. saccharalis to the new pyramided Bt maize technologies. RESULTS: A total of 382 F2 family lines derived from 735 feral larvae/pupae of D. saccharalis collected from six locations in Louisiana and Mississippi during 2008 and 2009 were screened for resistance to three new Bt maize technologies: MON 89034, Genuity? VT Triple Pro? and SmartStax?. None of the 382 F2 isoline families survived on the Bt maize leaf tissue for ≥ 12 days in the F2 screen. The joint frequency for two‐ or three‐gene resistance models with 95% probability in these populations was estimated to be < 0.0063 to MON 89034 and < 0.003 to VT Triple Pro? and SmartStax?. CONCLUSION: These results suggest that the resistance allele frequency in D. saccharalis to the three pyramided Bt maize technologies is low in the mid‐southern region of the United States, which should meet the rare resistance assumption of the currently used IRM strategy for Bt maize. Copyright © 2011 Society of Chemical Industry 相似文献
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J Cristian Vidal‐Quist Pedro Castañera Joel González‐Cabrera 《Pest management science》2010,66(9):949-955
BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) δ‐endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis serovar israelensis (Bti) solubilised protoxins showed biological activity against C. capitata neonate larvae, whereas Bti spore and crystal mixture was inactive. Insecticidal activity of Bti protoxins was significantly enhanced by incubation with Culex pipiens L. gut extracts. Overdigestion of Bti protoxins with Sesamia nonagrioides (Lefebvre) gut extracts suppressed biological activity against C. capitata, and this correlated with degradation of Cyt toxins. Cyt1Aa solubilised protoxin showed the highest toxicity, LC50 after 7 days of 4.93 µg cm?2, while proteolytical processing of Cyt1Aa protoxins by larval gut extracts did not enhance insecticidal activity. CONCLUSION: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single δ‐endotoxin with potential for controlling this pest. Copyright © 2010 Society of Chemical Industry 相似文献
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An outbreak of larvae and adults of a devastating moth was noted to be causing severe damage in tomato grown in greenhouses at Khartoum State, Sudan during June 2010. Symptoms included foliage mining and leaves drying. Photographs of symptoms and larvae and adults of the pest (and subsequently specimens) were sent to Russell IPM, UK and the Finnish Museum of Natural History for identification. The pest was identified as Tuta absoluta (Meyrick), (Lepidoptera: Gelechiidae). This is the first official record of T. absoluta in Sudan, based on surveys in tomato production areas in Khartoum, Gezira, Red Sea and North Kordofan States and eggplant and potato in Khartoum State. Severe yield loss due to the serious feeding damage on foliage and fruits infestation was reported on tomato and eggplant fields at Khartoum State. 相似文献
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Information on the mating and dispersal behaviour of the rice stem borers Scirpophaga incertulas and Chilo suppressalis is necessary for the design of resistance management strategies for rice varieties genetically engineered with toxin genes from Bacillus thuringiensis (Bt). We investigated whether S. incertulas and C. suppressalis move from the site of adult eclosion before mating to determine the likelihood that random mating will occur between adults eclosing in fields of Bt rice and refuge fields of non-Bt rice. Observations were made under laboratory, screenhouse and field conditions. Almost all S. incertulas males and females flew out of sight after cuticle expansion and hardening, as did most C. suppressalis. Approximately 15% of C. suppressalis females called and mated within 3?m of the site of eclosion, and approximately 5% of C. suppressalis males mated within 5?m of the site of eclosion. These results suggest that random mating is likely to occur between S. incertulas eclosing in Bt rice fields and nearby refuge fields. Although a small proportion of C. suppressalis did not move far before mating, random mating of moths from Bt and nearby refuge fields is also likely to occur in this species if Bt-resistant moths are rare. 相似文献
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Lack of fitness costs and inheritance of resistance to Bacillus thuringiensis Cry1Ac toxin in a near‐isogenic strain of Plutella xylostella (Lepidoptera: Plutellidae)
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Xun Zhu Yanjv Yang Qingjun Wu Shaoli Wang Wen Xie Zhaojiang Guo Shi Kang Jixing Xia Youjun Zhang 《Pest management science》2016,72(2):289-297
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BACKGROUND: Bacillus thuringiensis (Bt) and its protein crystals are used worldwide, either as a spray or when expressed in transgenic crops, for the control of pest insects. However, owing to their intensive use, there exists a debate regarding the involvement of this microbial insecticide in bee colony losses. In this study, in a tiered approach using laboratory microcolonies, an evaluation was made of the potential lethal and sublethal hazards on colony reproduction and foraging behaviour of workers of the bumblebee Bombus terrestris (L.) of two commercial Bt strains: kurstaki (Dipel®) and aizawai (Xentari®). Bumblebees, like honey bees, are intensively used in modern agriculture for pollination and fulfil a crucial role in the natural ecosystem. RESULTS: Exposure of bumblebees dermally or via treated pollen to either of the two Bt formulations at their field recommended rates (0.1%) caused no reduction in survival. However, when applied in the feeding sugar water, aizawai killed all workers at a concentration of 0.1%, but this lethal effect was lost at 0.01%. With respect to reproductive effects, kurstaki was harmless, while aizawai at 0.1% delivered in the feeding sugar water and pollen reduced reproduction by 100 and 31% respectively. Lower doses of 0.01% aizawai in the sugar water showed no more effect. In addition, kurstaki at 0.1% and aizawai at 0.01% in the feeding sugar water did not impair the foraging behaviour, resulting in normal nest colony performance. CONCLUSION: The results with kurstaki and aizawai demonstrated that, in general, the Bt strains are safe to B. terrestris bumblebees, although in some cases there were detrimental effects that depended on strain and route of exposure. In addition, the authors believe that to draw firm conclusions regarding the hazards of Bt to bumblebees would require more information on relevant concentrations of Bt products in the environment. Hence, routine testing for lethal and sublethal effects is recommended to ascertain combined use of Bt products and bumblebees in modern agriculture. Copyright © 2009 Society of Chemical Industry 相似文献
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Journal of Plant Diseases and Protection - A commercial formulation of Bacillus thuringiensis (Mattch) was evaluated under field conditions at Clemson, South Carolina, USA for control of the squash... 相似文献