Farmers' knowledge,perceptions, and management practices of the new invasive pest,fall armyworm (Spodoptera frugiperda) in Ethiopia and Kenya

This paper reports for the first time on farmers’ knowledge, perceptions and management practices of the fall armyworm (Spodoptera frugiperda) in Ethiopia and Kenya. A survey of 343 smallholder maize farmers was conducted. Most farmers in Ethiopia and Kenya had knowledge about fall armyworm; they could identify it mainly during its larval stage. Furthermore, most farmers in Ethiopia (93%) and Kenya (97%) encountered damage by fall armyworm in their farms. They estimated an average of 32% crop damage in Ethiopia and 47.3% of crop damage in Kenya, with an estimated yield reduction between 0.8 to 1 tonnes/ha. Nearly half of the farmers relied on chemical insecticides to control this pest. The majority (60%) of the farmers in Kenya perceived that insecticides were not effective in controlling fall armyworm as compared to most farmers (46%) in Ethiopia who perceived that chemical spray is effective for the control of fall armyworm. In Ethiopia, 26% of the farmers combined handpicking larvae with insecticide sprays, whilst 15% of the farmers practiced only handpicking. The present study highlights the need to develop management strategies for fall armyworm based on farmers’ needs and priorities.     

Impact of ant control technologies on insecticide runoff and efficacy

BACKGROUND: Insecticides are commonly used for ant control around residential homes, but post‐treatment runoff may contribute to contamination of surface water in urban watersheds. This study represents the first instance where runoff of insecticides was directly measured after applications around single family residences. During 2007, houses were treated with bifenthrin or fipronil sprays following standard practices. During 2008, pin stream applicators, spray‐free zones and restricting sprays to the house foundation were considered as management options. RESULTS: During 2007, the resulting runoff from the bifenthrin spray in the irrigation water had a mean concentration of 14.9 µg L^?1 at 1 week post‐treatment and 2.5 µg L^?1 at 8 weeks, both high enough to be toxic to sensitive aquatic organisms. In comparison, treatments with bifenthrin granules resulted in no detectable concentrations in the runoff water after 8 weeks. The mean concentration for fipronil used as a perimeter spray was 4.2 µg L^?1 at 1 week post‐treatment and 0.01 µg L^?1 at 8 weeks, with the first value also suggesting a potential for causing acute aquatic toxicity to sensitive organisms. During 2008, insecticide runoff was reduced by using spray‐free zones and pin stream perimeter applications. CONCLUSIONS: It is shown that insecticide runoff from individual home treatments for ants can be measured and used to improve techniques that minimize runoff. The pin stream application and applications limited to the house foundation should be further evaluated for their potential to reduce pesticide runoff from residential homes. Copyright © 2010 Society of Chemical Industry     

Field evaluation of reduced insecticide spray programs for managing plum curculio, Conotrachelus nenuphar (Coleoptera: Curculionidae), in Alabama peaches

BACKGROUND: Plum curculio (PC), Conotrachelus nenuphar (Herbst.), is an important pest of peaches in the southeastern United States. Commercially acceptable control of this insect is typically achieved by weekly or biweekly application of broad‐spectrum conventional insecticides, resulting in 6–12 sprays per season. Experiments were conducted in a peach orchard in Alabama during 2007–2009 to compare the conventional calendar‐based insecticide spray program involving weekly applications of phosmet with three different reduced spray programs using three targeted (well‐timed) insecticide sprays (TIS) of phosmet, permethrin or thiamethoxam applied in an alternated fashion. RESULTS: All three TIS programs significantly reduced PC damage at harvest compared with the untreated control in two of the three years (2008 and 2009). Fruit damage due to stink bugs, which are emerging pests of peaches in the region, was also significantly reduced in the TIS programs in both years. In a separate trial in which one of the TIS programs (three targeted sprays of phosmet) was evaluated in a larger peach block in 2009, percentage fruit damage due to PC increased from < 1% in June to ～4% in late July. CONCLUSION: All the TIS programs evaluated provided effective control of PC and represent potential alternatives to the conventional weekly spray program in peaches with concomitant reduction in insecticide usage and associated costs. However, an additional spray may be necessary for effective control of PC and stink bugs in late‐season peach varieties. Copyright © 2011 Society of Chemical Industry     

Biochemical and physiological changes on Bacillus thuringiensis cotton after imidacloprid foliar spray

Bacillus thuringiensis cotton is a variety of cotton genetically modified to contain a gene derived from B. thuringiensis bacteria; which results in expression of toxin protein that confers resistance to bollworm complex (the most destructive pest of cotton). Introduction of Bt cotton lowered the need of insecticides, but still a number of insecticides are used for other insects like jassids, whitefly, aphids and tobacco caterpillar to which Bt gene does not provide effective control. Imidacloprid (tradename Imidacel 17.8 SL) is an insecticide designed for control of these major sucking/piercing insects that affect cotton. So in the present work we studied the post effect of imidacloprid insecticide on plant health of three Bt cotton hybrids (RCH-134, JKCH-1947, NCEH-6R) as there are reports of this insecticide causing growth and yield enhancements in absence of insect pests. Imidacloprid was first sprayed at recommended concentration (40 ml/acre) on 3 months old plants sown in randomly designed plots with three replications of each hybrid. The spray was repeated three times at 10 days interval. The level of B. thuringiensis gene expression, peroxidase activity and total phenols was measured on third day after every spray in leaves along with growth and yield of plants. The insecticide has shown to increase the level of B. thuringiensis protein, peroxidase enzyme activity, total phenols, height, number of bolls retained on plants and yield. These observations suggested that the imidacloprid treated plants showed better growth and development, thereby imidacloprid has growth enhancing effect on Bt cotton plants in addition to its insecticidal properties.     

The use of insecticides to control potato leafroll virus in seed-potato crops on the Golan Heights

Experiments were carried out in the laboratory and in the field for 3 years, in an attempt to protect seed-potato crops on the Golan Heights from potato leafroll virus (PLRV) by means of insecticides. The best results with spray compounds were obtained with pirimicarb (Pirimor), methamidophos (Tamaron) and ethiofencarb (Croneton); the commonly used and recommended aphicides oxydemetonmethyl (Metasystox R) and endosulfan (Thionex) were less efficient. The best results with granular insecticides were obtained with aldicarb (Temik) and ethiofencarb, while phorate (Thimet), carbofuran (CURATERR) and oxamyl (Vydate) were much less efficient. Dimethoate (Rogor) gave good results in the laboratory test, but was less efficient in the field trials. The flight of aphids in the Golan Heights is characterized by two close peaks: in May-June, and from late July to September. Potatoes are planted in early May, and hence crops need to be protected throughout almost the entire season. Weekly sprays using, in turn, pirimicarb, methamidophos and ethiofencarb, reduced PLRV incidence in seed-potato plots in 1977 from 33.4% (in the untreated controls) to 2.8%, thus ensuring their certification. A single pre-emergence treatment with aldicarb reduced PLRV incidence from 12.6% (in the untreated controls) to 1.8%; however, the best results (no infection) were obtained by the addition of weekly sprays, starting at the end of July, to a pre-emergence treatment with a granular insecticide.     

防治水稻害虫新型杀虫剂硫环杀的试验研究

通过三年室内和田间试验,发现硫环杀对防治水稻害虫具有突出的优点,它与杀螟丹、杀虫双都属于同类新型结构的杀虫剂。硫环杀田间亩用量75克防治水稻二化螟、三化螟其效果与杀虫脒、杀螟丹、杀虫双在一个水平上。根区深层施药可兼治水稻多种害虫,并可提高防治效果。残效期可维持7～14天,田间施药1～2次可控制螟害,它不仅具有良好的内吸和触杀作用,还兼有胃毒和熏蒸作用。硫环杀属神经毒剂,对螟虫具有特殊的中毒症状及毒杀机制,对高等动物属中等毒性,无致癌、致突变作用,也不会引起畸胎的形成。根据硫环杀的理化特性、代谢、转化等残留分析,它积累在环境中或食物链上可能性不大,可以取代有机氯农药,其特殊的毒杀机制在克服害虫抗药性方面,可能作为轮换的一个新品种。它是一个很有希望的杀虫剂。     

Evaluation of fungicide application rates,spray schedules and alternative management options for rust and angular leaf spot of snap beans in Uganda

Yield losses due to rust and angular leaf spot (ALS) of snap beans may reach 100% in Eastern Africa. Where susceptible varieties are grown, farmers control these diseases with routine fungicide applications. To determine an optimum application rate and spray schedule for Orius® (tebuconazole 250 g/L), we sprayed 10 mL and 20 mL Orius® per 15 L spray water twice at two trifoliate leaf stage and 50% flowering, and three times at the same stages, with an additional application at pod initiation. In farmers’ fields, we tested the effect of fungicide sprays, use of resistant variety, intercropping, increased plant spacing and farmyard manure on rust and ALS diseases. Application three times of 20 mL Orius® per 15 L spray water reduced rust severity scores by 5.7 and 2.4 in 2010 and 2011, respectively. Lowest rust and ALS severities were observed when a resistant variety, fungicide or farmyard manure was used. Pod yield increments due to disease management ranged between 13% and 242%. Prophylactic fungicide application, use of resistant varieties and farmyard manure can be used to reduce disease severity and improve snap bean quality on smallholder farms.     

Motivating rice farmers in the Mekong Delta to modify pest management and related practices through mass media

A participatory planning process was applied to develop a media campaign to motivate rice farmers in the Mekong Delta to modify pest management practices together with seed and fertilizer inputs. Locally named ‘Ba Giam Ba Tang’ or ‘Three Reductions, Three Gains’, campaigns were launched in two provinces, Can Tho and Tien Giang. In both provinces, farmers' practices changed significantly. Their insecticide sprays reduced by 13–33% while their seed rates dropped ～10% and nitrogen rates, ～7% and proportion of farmers using insecticides declined by ～11%. These practices were supported by modifications in belief attitudes that favored high inputs. Farmers who reported significant reductions in the three inputs also changed their perception of yield loss. The campaigns in Can Tho and Tien Giang had significant multiplier effects. They stimulated several provincial governments as well as the Ministry of Agriculture and Rural Development to provide additional resources to reproduce the materials and campaign process for local use which eventually reached more than 3 million farmers in south and central Vietnam.     

Integrated management of insect pests,diseases and weeds of cotton in Africa

Integrated pest management systems were developed originally in response to the appearance of insect populations with resistance to common insecticides. Cotton with its heavy dependence on insecticidal sprays was one of the first crops in which the effectiveness of control declined due to resistance in the target pests. Although insecticide resistance is more of a problem in large scale production systems, the IPM approach can also benefit the smallholder by reducing the number of sprays required with consequent cost savings. Where crop growth is adversely affected by diseases, competition from weeds or poor management, the full benefit of insecticide spray programmes cannot be realised. To be fully effective, insect control should therefore be integrated with other crop protection activities. This paper reviews the insect pests, diseases and weed problems of cotton in Africa and discusses the possibilities for extending the principles of IPM to cover all the crop protection activities with practical examples drawn from both small scale and larger scale production systems.     

Influence of pesticide information sources on citrus farmers' knowledge,perception and practices in pest management,Mekong Delta,Vietnam

In 1998-99, about 150 citrus farmers and 120 pesticide sellers were interviewed in Can Tho and Dong Thap province, Mekong Delta, Vietnam. Media, pesticide sellers and extension staff had different influences on farmers' pest perception and management practices depending on the region and intensity of the cropping system. Pesticide sellers were notified by about 95% of the farmers about their major pest problems, and the type of pesticides sold in their shop was primarily based on farmers' demand (87%) and then on company promotion (56%). Those farmers relying on pesticide sellers used more of the banned insecticide methyl parathion. Probably for fear of being accused of illegal practices, none of the pesticide sellers mentioned that they recommended this product or that farmers asked for it. In the intensive Tieu mandarin cropping system, media and extension activities increased farmers' knowledge of difficult-to-observe pests such as the citrus red mite Panonychus citri and thrips, Thrips sp. and Scirtothrips sp. Since extension was weak in sweet orange, those farmers exposed to media only reported the damage symptom of mites, not knowing the causal agent. Media alone seemingly did not suffice to acquaint farmers with these small organisms. Farmers getting advice from the media advertisements applied more different pesticide products and sprayed insecticides more frequently, whereas the extension has stimulated the use of acaricides and increased the number of both insecticide and fungicide sprays. The traditional practice of biological control with the ant Oecophylla smaragdina might be endangered with growing media influence and when extension activities remain confined to chemical pest control. Constraints and potentials of different information sources are discussed in relation to developing IPM programmes for citrus.     

The persistence of insecticide spray deposits on woven polypropylene and jute sacking

In a trial, simulating the spray treatment of bags, the persistence and biological activity of insecticide spray deposits on jute and woven polypropylene sheets have been compared; in addition, the build-up of residues in thin layers of maize under the sprayed sheets has been measured. The insecticides were found to have a much shorter persistence on polypropylene than on jute and this was coupled with higher residues on the grain. However, the deposits on polypropylene retained equal or greater activity against Sitophilus zeamais Motsch. and Tribolium castaneum (Herbst) than the deposits on jute, despite the loss of surface deposit. Wettable powder formulations had a slightly greater surface persistence than emulsifiable concentrates and resulted in smaller residues in the grain. There would seem to be more usefulness in using insecticide sprays on woven polypropylene than on jute bags, and a wettable powder formulation of a non-volatile insecticide of low mammalian toxicity would be the best choice. Of the insecticides tested, the pyrethroid permethrin (cis: trans ratio= 25:75) was the most effective and gave the least residue in the grain.     

Evaluation of food sprays and relay strip crops for enhancing biological control of bollworms and cotton aphids in cotton

Studies were conducted at the Texas Agricultural Experiment Station research farms located at Munday (1996 test) and Chillicothe (1997 test) to evaluate relay strip crops in combination with a food spray to enhance biological control of bollworms, Helicoverpa zea (Boddie), and cotton aphids, Aphis gossypii Glover, in cotton. The relay crops included fall plantings of hairy vetch, Vicia villosa Roth, and canola, Brassica napus L., and a spring planting of grain sorghum, Sorghum bicolor L. Cotton, Gossypium hirsutum L., was planted between the relay crops or was isolated from the relay crops. Treatments within the two cotton systems included an untreated check plot, a plot sprayed with sugar+yeast (food spray) during summer to attract and hold predator insects, a plot sprayed with biological ('soft') insecticides for bollworm and cotton aphid control (Bacillus thuringiensis and pymetrozine, respectively), and a plot sprayed with harsh insecticides for bollworm and cotton aphid control (zeta cypermethrin and dicrotophos or profonofos, respectively). A split-plot experimental design, with three replications, was used where whole plots included relay and isolated cotton systems and subplots were the four food/chemical treatments. Predator numbers were monitored with a vacuum sampler once a week in relay crops and cotton. Bollworms and cotton aphids were monitored visually once a week in cotton during July and August. Total predator numbers were higher in cotton adjacent to relay crops in 1996, but not in 1997. The food spray did not enhance attraction and retention of predators either year. Bollworm larval numbers were significantly higher in relay cotton, food spray plots in 1996. Bollworm larval numbers were similar in relay and isolated cotton, and larval numbers were significantly reduced only in the plot where zeta cypermethrin (harsh insecticide plot) was used. In 1996, cotton aphid numbers in the relay cotton system were significantly higher in the untreated check plots in relation to numbers in the food spray, soft insecticide, and harsh insecticide plots, which were statistically similar. In the isolated cotton system, aphid numbers were highest in untreated plots, intermediate in food spray and soft insecticide plots, and lowest in the harsh insecticide plots. Aphid numbers increased more rapidly in the harsh insecticide plots that had been treated previously for bollworm control. The food spray and pymetrozine treatments reduced cotton aphids more effectively in the relay cropping system than in the isolated cotton system. Bollworms and cotton aphids did not reach pest status in 1997. The combination of a relay cropping system with a food spray did not enhance predator numbers and did not aid in retention of predators in cotton during August. Sucrose in the food spray attracted high numbers of bollworms in 1996.     

Development of an integrated pest management programme for cotton. Part 2: Integration of a lucerne/cotton interplant system,food supplement sprays with biological and synthetic insecticides

Part 1 of the paper showed that the integration of a lucerne/ cotton interplant system with supplementary food sprays retained and increased populations of predatory insects in the cotton crop. The strategic use of biopesticides (Bacillus thuringiensis (Bt), nuclear polyhedrosis virus (NPV)) reduced the use of synthetic insecticides by 50% without sacrificing cotton yield and profitability. In economic terms, the average gross margin for the IPM plot was A$3255 compared with A$3020 and A$3218 for the plots treated with (1) conventional insecticides on transgenic (Ingard®) cotton and (2) conventional insecticides on non-transgenic cotton. The use of transgenic cotton affected a 25% saving on synthetic insecticide usage. The results clearly indicate that a refined IPM approach could have a distinctive advantages in terms of economic and environmental impacts on cotton production. The success of such an approach lies in a strict adherence to its methodology.     

Development of a bioassay system for monitoring susceptibility in Thrips tabaci

A system is described for collecting adult and larva of Thrips tabaci from onion foliage into insecticide-treated vials to evaluate susceptibility to insecticides. The thrips insecticide bioassay system (TIBS) allows one to treat vials and store them for 3 weeks before thrips are collected. Depending on the population density in the field, collection of the insects for the test required from 3-6 h for one person. Assays are read after 24h. This system was used in 1997 and 1998 in commercial onion fields in Honduras and Nicaragua, and TIBS was sensitive enough to detect differences to the insecticides tested, to thrips life stages and to different generations within an onion-growing season. Data collected suggest that there were not serious problems with thrips insecticide resistance, with the possible exception of cypermethrin in Nicaragua which had a resistance ratio (RR) value of 26.9 for adult thrips. The largest RR values were observed at the end of the growing season, and this may be caused by the season-long selection by insecticide sprays. The mortality of adults and larvae followed the same general pattern, but the ratio between larvae and adults differed for each chemical group.     

Topical and residual toxicity of six pesticides to <Emphasis Type="BoldItalic">Orius majusculus</Emphasis>

The toxicity of six pesticides (four insecticides and two fungicides) to Orius majusculus (Reuter) (Hemiptera: Anthocoridae) adults and nymphs was determined using different exposure methods. Mortality upon topical exposure to abamectin, endosulfan and spinosad at recommended field doses ranged from 56% to 100% after 24 h. However, in leaf residue tests, toxicity to both life stages decreased significantly, ranging from 0% to 33% mortality. Benomyl and copper salts + mancozeb (fungicides) were much less toxic to O. majusculus, with less than 15% mortality of either adults or nymphs in topical and residue bioassays. In persistent toxicity tests, insects were exposed to the same three insecticides for 4 days; mortality varied from 38% to 100%. Egg hatching was not significantly affected when abamectin, endosulfan and spinosad were topically applied. Number of eggs laid per female in choice and no-choice tests did not differ significantly from the control. The insecticides did not show considerable repellent effect in the choice tests. Topical, residue and systemic uptake methods were also compared to determine the differences in the toxicity levels of imidacloprid, a systemic insecticide. However, 100% mortality occurred with all methods.     

Time-controlled cholinesterase reactions as a means of measuring water-soluble residues of organophosphorus insecticide spray deposits on plant surfaces

Ellman's method of cholinesterase assay which is often used for residue analysis of organophosphorus insecticides has been modified by adding the protein denaturant, sodium dodecyl sulphate, to control the duration of the reaction. In doing so, the reaction time can be standardised to facilitate the measurement in a field laboratory of residues of water-soluble organophosphates, particularly on plant surfaces that have been subjected to sprays. Thirty samples can be analysed in 1 h by timing each step of the procedure for a series of 30 reaction tubes at 1-min intervals from the incubation of the enzyme with the insecticide to the final addition of the sodium dodecyl sulphate. Results from a series of aerial spray trials are presented and discussed briefly as an example of a field application of the method.     

The role of glutathione S-transferases in the detoxification of some organophosphorus insecticides in larvae and pupae of the yellow mealworm,Tenebrio molitor (Coleoptera: Tenebrionidae)

The correlation between the natural levels of glutathione S-transferase (GST) and the tolerance to the organophosphorus insecticides parathion-methyl and paraoxon-methyl, as well as the interaction of affinity-purified enzyme and the insecticides were investigated in order to collect further information on the role of the glutathione S-transferase system as a mechanism of defence against insecticides in insects. The studies were carried out on the larvae and pupae of the coleopteran Tenebrio molitor L, which exhibit varying natural levels of GST activity. Stage-dependent susceptibility of the insect against insecticides was observed during the first 24 h. However, 48 h after treatment, the KD₅₀ value increased significantly due to the recovery of some individuals. Simultaneous injection of insecticide with compounds which inhibit GST activity in vitro caused an alteration in susceptibility of insects 24 or 48 h post-treatment, depending on stage and insecticide used. Inhibition studies combined with competitive fluorescence spectroscopy revealed that the insecticides probably bind to the active site of the enzyme, thus inhibiting its activity towards 1-chloro-2,4-dinitrobenzene in a competitive manner. High-performance liquid chromatography and gas chromatography revealed that T molitor GST catalyses the conjugation of the insecticides studied to a reduced form of glutathione (GSH). From the above experimental results, it is considered that GST offers a protection against the organophosphorus insecticides studied by active site binding and subsequent conjugation with GSH. © 2001 Society of Chemical Industry     

Contact and fumigant toxicity of plant essential oils and efficacy of spray formulations containing the oils against B‐ and Q‐biotypes of Bemisia tabaci

BACKGROUND: The contact + fumigant toxicity of 92 plant essential oils and control efficacy of 18 experimental spray formulations containing nine selected essential oils (0.5 and 0.1% sprays) and six commercial insecticides to females from B‐ and Q‐biotypes of Bemisia tabaci were evaluated using vapour‐phase mortality and spray bioassays. RESULTS: Garlic and oregano (LC₅₀, 0.15 mL cm^?3) were the most toxic oils against B‐ and Q‐biotype females. Strong fumigant toxicity to both biotype females was also obtained from catnip, cinnamon bark, clove bud, clove leaf, davana, savory and vetiver Haiti oils (LC₅₀, 0.17–0.48 mL cm^?3). The 0.5% sprays of these oils (except for thyme red oil) resulted in 90–100% mortality against both biotype females. Only garlic applied as 0.1% spray provided 100% mortality. Spinosad 100 g L^?1 suspension concentrate (SC) treatment resulted in 92 and 95% mortality against both biotype females, whereas acetamiprid 80 g L^?1 wettable powder (WP), imidacloprid 80 g L^?1 SC, thiamethoxam 100 g L^?1 water‐dispersible granule (WDG) and pyridaben 200 g L^?1 WP treatments resulted in 89–100% mortality against B‐biotype females only. CONCLUSION: In the light of global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment, the essential oils described, particularly garlic, cinnamon bark and vetiver Haiti, merit further study as potential insecticides for the control of B. tabaci populations as fumigants with contact action. Copyright © 2011 Society of Chemical Industry     

棉花结铃动态与棉红铃虫发生的相互关系

农业生态系统的结构是以作物群体为中心形成的,害虫的综合防治问题应与作物生产紧密结合。了解棉花生长和发育是设计与执行棉虫综合防治不可忽视的部分。棉红铃虫的防治措施不仅要根据蛾量,而且更重要的应考虑棉株发育时期。如果红铃虫蛾量达     

根区施药试验研究的新进展

本文就继续研究应用呋喃丹、杀虫脒、巴丹类等内吸杀虫剂根区施用的十种不同方式防治水稻三化螟、稻瘿蚊、稻蓟马、稻纵卷叶螟、褐稻虱等主要害虫的药效及对害虫天敌影响的新进展作扼要的报道,进一步阐明根区施药的科学根据、盆栽及田间试验结果、以及指出应用楝科植物(印楝、川楝、苦楝)对稻瘿蚊等害虫防治的可能性和探索呋喃丹根区施用对褐稻虱的杀卵作用。此外,对湖北、江西等地约10万余亩稻田推广根区施药试验示范的情况亦作适当的介绍。