Changes in external and internal color during postharvest ripening of ‘Manila’ and ‘Ataulfo’ mango fruit and relationship with carotenoid content determined by liquid chromatography–APcI-time-of-flight mass spectrometry

High-performance liquid chromatography–atmospheric pressure chemical ionization (APcI⁺)-time-of-flight mass spectrometry studies revealed that all-trans-β-carotene and the dibutyrates of all-trans-violaxanthin and 9-cis-violaxanthin were the main carotenoids in ‘Ataulfo’ and ‘Manila’ mango fruit mesocarp. The concentration of these carotenoids in the mesocarp was measured during fruit ripening and correlated with colorimetric changes of mesocarp and epidermis. The lowest and highest concentrations of all-trans-β-carotene, all-trans-violaxanthin and 9-cis-violaxanthin (as dibutyrates) during the ripening of ‘Manila’ mango were 0.25 × 10⁻³ to 35.57 × 10⁻³, 0.40 × 10⁻⁵ to 31.97 × 10⁻³ and 0 to 16.81 × 10⁻³ g kg⁻¹ of fresh mesocarp, respectively. For ‘Ataulfo’ they were 2.55 × 10⁻³ to 39.72 × 10⁻³, 0.16 × 10⁻³ to 15.00 × 10⁻³ and 0.21 × 10⁻³ to 7.48 × 10⁻³ g kg⁻¹ of fresh mesocarp, respectively. The concentration of these carotenoids increased in an exponential manner during fruit ripening in ‘Ataulfo’ and in an exponential or second-order polynomial manner in ‘Manila’. The highest correlation coefficients were obtained for the relationships between the mesocarp and epidermis a* and h° color values and the concentration of the evaluated carotenoids in both mango cultivars (R = 0.81–0.94). Equations to predict the concentration of the most important carotenoids in ‘Manila’ and ‘Ataulfo’ mango fruit on the basis of their mesocarp and epidermis color values were obtained.     

Expression of expansin genes during postharvest lignification and softening of ‘Luoyangqing’ and ‘Baisha’ loquat fruit under different storage conditions

Four expansin cDNA fragments, EjEXPA1, EjEXPA2, EjEXPA3 and EjEXPA4, were isolated and characterized from loquat (Eriobotrya japonica Lindl.) fruit. EjEXPA1 mRNA accumulated consistently with the increase in fruit firmness in 0 °C storage of ‘Luoyangqing’ (LYQ) fruit, where chilling injury with increased fruit firmness due to lignification was observed. EjEXPA1 mRNA levels were lower in fruit that underwent low temperature conditioning (LTC, 6 d at 5 °C then 4 d at 0 °C), and in 1-methylcyclopropene (1-MCP) treated fruit, in both cases where chilling injury was alleviated. Fruit of the ‘Baisha’ (BS) cultivar soften after harvest rather than increase in firmness, and high expression levels of EjEXPA1 and EjEXPA4 accompanied the softening of BS fruit stored at 20 °C; such mRNA accumulation was much lower when fruit were stored at 0 °C, where softening was significantly inhibited by the low temperature. Very low expression of EjEXPA2 and EjEXPA3 was observed during storage of both LYQ and BS fruit under the different storage conditions. Our results showed that of the four genes characterized, EjEXPA1 might be associated with chilling-induced lignification while both EjEXPA1 and EjEXPA4 were closely related to softening of loquat fruit during the postharvest period.     

Assessing the ‘true impact’ of development assistance in the Gaza Strip and Tokelau: ‘Most Significant Change’ as an evaluation technique

The democratic evaluative tradition has sought to change evaluation practice towards approaches and techniques that generate diverse forms of knowledge and foster public deliberation over a programme's merit and worth. This paper locates one evaluation method, ‘Most Significant Change’ (MSC), within this tradition. Drawing on two different evaluations – one, of a comprehensive economic sector assistance package to the Government of Tokelau, and the other of a psychosocial and academic support intervention for pre‐adolescent children in conflict‐affected regions of the Gaza Strip – the paper provides evidence of how MSC can capture unexpected outcomes, act as a tool for real‐time formative learning, and expose the competing theories, logics and values behind programme activity. The examples within the paper also provide evidence of how MSC begins to redistribute traditional power relationships in assessing the merit and worth of observed impacts by increasing the legitimacy of local programme knowledge, and engaging all parties in evaluative decisions. By doing so, MSC, the paper argues, better serves the purposes of learning, improvement and mutual accountability which should sit at the core of good development practice.     

Extreme air pollution as a focusing event: A case study of the ‘Airpocalypse’ in Beijing,January 2013

Sudden events of crisis (‘focusing events’) trigger intense media reporting, which in turn can prompt political decision‐makers to respond with an adjustment of existing policies. As such, focusing events can potentially lead to long‐term policy change on a certain issue. This article explores the sudden and intense period of air pollution in Beijing in January 2013 as a focusing event. The episode, which international media referred to as ‘Airpocalypse’, triggered widespread discussion and policy adjustments that can be assumed to have accelerated the control of air pollution in China. The ‘Airpocalypse’ was not the first event of intense air pollution in Beijing, but the only one which seemingly caused lasting sociopolitical changes. The aim of this article is to explore the underlying situational factors that enabled the ‘Airpocalypse’ to be more relevant than other events of severe air pollution. This paper argues that a combination of historically high pollution, improved access to information, increasing government willingness to address the issue, and the ability of media to more openly discuss air pollution created a public pressure situation to which the government was prompted to respond in a stronger way than during previous events of heavy air pollution.     

‘Friendly islands’ in an unfriendly system: Examining the process of Tonga's WTO accession

Tonga became the 151st member of the World Trade Organization (WTO) in July 2007. This article considers the issues that Tonga faced during its WTO accession process, and the potential impact of the reforms it has made in compliance with the terms of its accession. It notes that WTO membership may offer some benefits to Tonga, including improved market access for its exports and the provision of technical assistance. The taxation and regulatory reforms necessitated by accession might also have some positive development effects. However, this article argues that the costs of WTO accession outweigh these benefits. This is because Tonga faced numerous challenges during its accession process, which led it to agree to substantial trade concessions. In addition, the ‘special and differential’ (S&D) treatment provisions intended to assist developing countries like Tonga once they become WTO members are inadequate. While this article focuses on Tonga, the issues raised are relevant to other Pacific SIDS engaged in WTO accession negotiations, including Vanuatu and Samoa.     

Epicuticular wax content and morphology as related to ethylene and storage performance of ‘Navelate’ orange fruit

The effect of ethylene (2 μL L⁻¹) on total and soft epicuticular wax content and wax morphology has been investigated in mature ‘Navelate’ (Citrus sinensis, L. Osbeck) oranges held under non-stressful environmental conditions (22 °C and constant high relative humidity (90–95% RH)). In addition, the objective of the study was to understand whether the ethylene-induced changes in epicuticular wax might participate in the beneficial effect of ethylene reducing non-chilling peel pitting, by modifying peel water, osmotic or turgor potential, or disease incidence caused by Penicillium digitatum (Pers.:Fr.) Sacc. Ethylene increased total and soft epicuticular wax content in ‘Navelate’ fruit and induced structural changes in surface wax that might be related to the formation of new waxes. Changes in epicuticular wax morphology, but not in its content, might be involved in the protective role of ethylene reducing non-chilling peel pitting, although the beneficial effect of the hormone is not related to water stress. Cell water and turgor potentials in freshly harvested fruit and fruit stored in air under non-stressful conditions suggest that water stress is not a limiting factor leading to the development of this physiological disorder. In addition, the results indicated that formation of new waxes in fruit treated with ethylene may partially cover stomata, cracks or areas lacking wax occurring in stored fruit and is likely to improve physical barriers to P. digitatum penetration.     

‘Changing My Thinking With a Western Woman’: Javanese Youths’ Constructions of Masculinity

Yogyakarta’s ‘wild’ guides are young men who pursue fantasies of true love and easy wealth by striking up ‘love affairs’ with tourist women. A hegemonic masculinity emerges as male peergroup gossip evaluates dramatic incidents to pinpoint ideal male with­male image and behaviour. In romances with Western women, guides renegotiate the sexual aspect of their masculinity, as tourist women’s holiday­romance expectations of attentiveness and deference collide with this ideal masculine self­image. The tension between disgust and desire within guides’ lifestyle leads to fragmented performances of identity for their different audiences.     

‘The Taste of Paradise’: Selling Fiji and FIJI Water

Abstract: Effective global competitiveness is rare in the Pacific islands, yet FIJI Water has been a major success story since 1997, exporting bottled water to the USA and elsewhere. A bland commodity has been linked to an ‘exotic’ place, and sold to elite consumers, as a form of cultural capital. The company website and newspaper extol the virtues of a ‘pristine’ product, produced in a natural context, in an environmentally sensitive manner. Marketing these themes and product placement have enabled success in a highly competitive market. Place has been used as a means of marketing perceived taste, distinctiveness and quality.     

Effect of high-pressure hot-water washing treatment on fruit quality, insects, and disease in apples and pears: Part I. System description and the effect on fruit quality of ‘d’Anjou’ pears

A manually operated high-pressure hot-water washing system consisting of a boiler, hot-water mixing tank, contact loop, heat exchanger, spray mixing tank, high-pressure hot-water washing manifold, low-pressure fresh water rinse manifold, and pressure pump was constructed and installed in a packingline. The system developed 20–50 °C washing water at pressures up to 980 kPa. ‘d’Anjou’ pears (Pyrus communis L.), shortly after harvest, and after storage for 3 and 4 months in regular air (RA) or for 4, 7 and 8 months in controlled atmosphere (CA) at −1 °C were washed through the packingline with different wetting agents (0.1% Silwet, 0.01 and 0.1% Defoamer, and water), water pressures (regular and high-pressure (210–980 kPa)), water temperatures (control (tap water, 4–22 °C), 40 °C, and 50 °C), and brushes (soft and firm), respectively. The effect of the washing conditions on fruit quality was investigated after 1 month of storage at −1 °C to simulate shipping condition, and then again after 1 week at 20 °C to simulate marketing condition. Hot-water caused severe heat scald. When nozzle temperature was 50 °C, the incidence of heat scald increased to over 50% for the fruit stored in RA for 3 months. Combined with hot-water, 540 kPa high-pressure washing increased the incidence of friction discoloration. There were lower incidences of friction discoloration and heat scald for fruit stored in CA for 7 months, in comparison to that in RA for 3 months. However, those fruit did not ripen properly as indicated by a high extractable juice content. Fruit washed at harvest had minor incidences of friction discoloration regardless of different brushes, water pressures, and wetting agents. Fruit washed after storages in either 4 months RA or 4 or 8 months CA suffered a high incidence of friction discoloration including scuffing symptoms and pressure marking. The firm brushes caused a higher incidence of friction discoloration mainly because of scuffing symptoms. However, no differences were found between different water pressures and wetting agents with respect to friction discoloration. Fruit stored for 4 months RA suffered 26–28% friction discoloration in comparison to 16–18% in CA stored fruit with firm brush washing. Extended CA storage increased friction discoloration even with soft brush washing. The results suggest that a washing system with high-pressure spray, <30 °C warm water, wetting agent Defoamer and rotating soft brushes were significantly effective in removing surface pests and decay control without causing internal or external damage of fruit.     

The effect of delays in establishment of a static or dynamic controlled atmosphere on the quality of ‘Hass’ avocado fruit

The effect of delays of 1, 5, 10 or 15 d after harvest in establishing a static controlled atmosphere (SCA) or dynamic controlled atmosphere (DCA) on the quality of ‘Hass’ avocados (Persea americana Mill.) was investigated. Fruit were stored at 5 °C in SCA (5% O₂/5% CO₂) or DCA (<3% O₂/0.5% CO₂) for 6 weeks and compared with fruit stored in air. In addition, to determine whether increasing the CO₂ in the DCA would affect the fruit quality, DCA-stored fruit were compared with fruit held in a DCA with 5% CO₂ (DCA + CO₂) established 1 d after harvest. The quality of fruit was assessed at the end of storage and after ripening at 20 °C. DCA-stored fruit ripened in 4.6 d compared with 7.2 d for SCA-stored fruit, or 4.8 d for air-stored fruit. In addition, the incidences of stem end rot (SER), body rot (BR) and vascular browning (VB) were lower in DCA-stored fruit (35%, 29% and 29%, respectively) than in SCA-stored fruit (57%, 52% and 49%, respectively), or air-stored fruit (76%, 88% and 95%, respectively). Delaying the establishment of both SCA and DCA for 15 d resulted in significantly more advanced skin colour at the end of storage (average rating score 11.9) compared with other delay periods (4.6–5.1). There was no significant effect of delay on the time to ripen, skin colour when ripe or any ripe fruit disorder incidence. The incidence of diffuse flesh discolouration (DFD) was not only <1% when averaged over all delays but only occurred at >0.5% incidence in the 15 d delay treatment in DCA (4.8%) and not in SCA. The incidence of diffuse flesh discolouration was 62% in air-stored fruit. Inclusion of 5% CO₂ in DCA retarded fruit ripening from 4.7 to 6.9 d and increased the incidence of rots at the end of storage from 5% to 14%, and increased the incidence in ripe fruit of SER from 30% to 56% and of BR from 27% to 55%. It is concluded that fruit quality was better after CA storage than after air storage, and that DCA storage was better than SCA. The effect of DCA is to independently reduce the time to ripen after storage and the incidence of rots when ripe. Delaying the application of SCA or DCA did not affect the expression of rots, but may increase the incidence of DFD. Inclusion of CO₂ at 5% in CA retarded fruit ripening but stimulated rot expression and should not be used for CA storage of New Zealand grown ‘Hass’ avocados.     

‘CPAN 1842’: a new source of adult plant leaf rust resistance in bread wheat

There is worldwide interest in adult plant resistance (APR) because of greater durability of APR to the cereal rusts. Peruvian bread wheat genotype ‘CPAN (Coordinated Project Accession Number) 1842’ (LM 50–53) has shown leaf rust resistance in disease screening nurseries since its introduction in 1977. However, it is susceptible at the seedling stage to several Puccinia triticina (Pt) pathotypes including the widely prevalent 77‐5 (121R63‐1) that infects bread wheat. Inheritance studies showed that CPAN 1842 carried a dominant gene for APR to pathotype 77‐5, which was different from Lr12, Lr13, Lr22a, Lr34, Lr35, Lr37, Lr46, Lr48, Lr49 and Lr68, based on the tests of allelism; and from Lr67, based on genotyping with the closely linked SSR marker cfd71. This gene should also be different from Lr22b as the latter is totally ineffective against pathotype 77‐5. CPAN 1842 therefore appears to be a new promising source of leaf rust resistance. Also having resistance to stem rust and stripe rust, this line can contribute to breeding for multiple rust resistances in wheat.     

The ‘street politics’ of migrant il/legality: Navigating Malaysia's urban borderscape

Immigration control constitutes a particular technique for regulating urban space and for controlling and disciplining migrant subjects within it. Unlike other manifestations of state power in exemplary urban settings, the architecture of urban immigration control is not recognisable through grand buildings or walls, but rather through its momentary presence and continuously shifting location: ad hoc identity controls in public spaces, roadblocks in neighbourhood streets or raids against workplaces. Building on fieldwork conducted in the Malaysian city of George Town, this article takes an interest in how migrants navigate this urban borderscape in order to avoid exploitation and encounters with the police. Read through Asef Bayat's notion of ‘street politics’, the article shows how migrants use the means (made) available to them in order to extend their room to manoeuvre. While such tactics are often driven by the force of necessity, they do nonetheless cumulatively encroach on the state's ability to produce migrants as (un)wanted or even (il)legal subjects in the city. Through this, migrants also challenge the very notion of what an exemplary urban space is as well as who is considered a legitimate part of it.     

The idea of ‘Country’: Reframing post‐disaster recovery in Indigenous Taiwan settings

In Australian Aboriginal thinking, the idea of ‘Country’ comprises complex ideas about relationships and connection. It simultaneously encompasses territorial affiliation, a social identification and cosmological orientation. It draws attention to what might be glossed as people‐to‐environment, people‐to‐people and people‐to‐cosmos relations. These relations influence disaster responses, but are rarely mobilised explicitly in shaping formal recovery and reconstruction efforts. Colonial disruption of connections to Country imposed new practices and presences into contemporary Indigenous geographies and is often reinforced in disaster settings. This paper considers more recent disruptions arising from post‐disaster recovery in Taiwan, arguing that the idea of Country offers a powerful way of framing cultural and social dimensions of post‐disaster relief and recovery for government agencies, non‐government organisations and research alike.     

Openings in the body of ‘capitalism’: Capital flows and diverse economic possibilities in Kiribati

Much of the post‐development agenda is concerned with decoupling Eurocentric imaginings of development from development practices in ‘remote’ regions and exploring new forms of economy that can enhance local well‐being. In the South Pacific (and elsewhere), small peripheral economies have confronted globalisation in varying ways. Some places, such as the Micronesian island state of Kiribati, have engaged directly with the global economy by investing capital generated locally in international financial markets rather than in domestic industries. Kiribati's trust fund, the Revenue Equalisation Reserve Fund, maintains a balanced portfolio of international equity and fixed income assets that produces a financial return, helping to augment Kiribati's other national income sources. In this paper we explore the results of capital flowing from Kiribati to global financial markets, noting that this alternative development practice can enhance local well‐being.     

More than a ‘blip’: The changed character of South‐East Asia’s engagement with the global economy in the post‐1997 period

Abstract: Through reviewing South‐East Asia’s recent economic engagement with the global economy, this paper points to profound recent transformations in the scope and character of the region’s development. Foreign direct investment was the key driver of regional growth in the pre‐1997 period, yet currently, the region faces a more difficult and multifaceted economic arena from which to attract such funds. Foreign direct investment is more selective, both geographically and by sector, than was the case in that earlier period. This paper explains the confluence of economics, business practices and politics that are giving rise to these outcomes, and concludes from this that development trajectories in South‐East Asia will become more diverse between the countries of the region, with implications for how we understand regional economic performance.     

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N. tabacum (N. tabacum L. cv. (gla.) S ‘K326’), was successfully crossed with N. alata. Despite a high DNA content variability, F₁ hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F₁ plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F₁ subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum.