Restoring Tallgrass Prairie and Grassland Bird Populations in Tall Fescue Pastures With Winter Grazing

Restoration of grasslands dominated by tall fescue (Schedonorus phoenix [Scop.] Holub) to native tallgrass prairie usually requires burning, herbicides, or reseeding. We tested seasonal grazing by livestock in winter, combined with cessation of fertilization, as a restoration tool for modifying the competitive dynamics among herbaceous plants to restore tallgrass prairie communities in southeastern Kansas. In 2004–2005, we compared responses of grassland plants and birds across a chronosequence of pastures that were winter-grazed from 1 yr to 5 yr. We compared winter-grazed pastures to pastures grazed year-round and to local native prairie remnants as starting and endpoints for restoration, respectively. Abundance of native warm-season grasses increased from 2% to 3% mean relative frequency in pastures grazed year-round to 18% to 30% in winter-grazed pastures, and increased with duration of winter-grazing. Native warm-season grasses accounted for 1–6% of total live aboveground biomass in pastures grazed year-round, 1–34% in winter-grazed pastures, and 31–34% in native prairie remnants. Tall fescue abundance and biomass were similar among grazing treatments, with a trend for tall fescue to be less dominant in winter-grazed pastures. Tall fescue made up 9–40% of total aboveground biomass in year-round grazed pastures and 10–25% in winter-grazed pastures. Grassland birds showed variable responses to winter-grazing. Dickcissels (Spiza americana) and Henslow’s sparrows (Ammodramus henslowii) were more abundant in winter-grazed pastures, whereas eastern meadowlarks (Sturnella magna) and grasshopper sparrows (A. savannarum) had similar abundance in pastures grazed year-round and during winter. Winter-grazing of pastures dominated by tall fescue combined with suspension of nitrogen fertilization could be an effective restoration technique that allows use of prairie rangeland while improving habitat for sensitive grassland birds.     

补播禾草对退化苜蓿草地生产性能及牧草品质的影响

针对宁夏半干旱区7龄以上苜蓿(Medicago sativa)草地退化导致的牧草产量低、品质差等问题,本试验采用单因素随机区组设计,研究了多年生黑麦草(Lolium perenne)、披碱草(Elymus nutans)、苇状羊茅(Festuca arundi-nacea)、无芒雀麦(Bromus inermis)、鸭...     

不同前茬对混播草地建植初期的影响

选择白三叶(Trifolium repens)、紫花苜蓿(Medicago sativa)、苇状羊茅(Festuca arund inacea)和鸭茅(Dactylisg lomerata),在三峡库区沿江地带3种前茬分别为水稻(Rice)、沟叶结缕草(Manila grass)和黄豆(Soybean)的退耕地进行混播试验,研究当年的生长动态、产草量组成及其越夏率。结果表明:禾本科牧草与白三叶混播,产草量显著高于与苜蓿的混播组合,且产草量比重相对稳定;苜蓿竞争性差,不适宜混播;土壤条件显著影响混播效益及其产草量;结缕草茬的土壤肥力低,禾本科牧草长势差,有利于豆科牧草生长,其产草量显著低于其它两种前茬;黄豆茬土壤肥力好,禾本科牧草早期生长旺盛,将抑制豆科牧草;水稻茬的混播组合相对稳定;土壤肥力直接影响混播牧草的越夏率;伏旱来临之前,良好的草层盖度有利于牧草越夏。     

Water-Soluble Carbohydrates of Cool-Season Grasses: Prediction of Concentrations by Near-Infrared Reflectance Spectroscopy and Evaluation of Effects of Genetics,Management, and Environment

An excess of water-soluble carbohydrates (WSCs) can present a risk for horses prone to pasture-associated laminitis or some other metabolic conditions. Determining WSC concentrations in commonly grazed cool-season grasses, at different times of day and under different fertilization treatments, can help optimize grazing times and management strategies. The goals of this study were to develop a near-infrared reflectance spectroscopy (NIRS) calibration curve for WSC and to apply it to four cool-season grass species. Tall fescue, Kentucky bluegrass, orchardgrass, and perennial ryegrass (10 cultivars) were sampled every two to four weeks from plots with or without added nitrogen, in the morning and afternoon. WSCs were quantified colorimetrically for a sample subset, and these values were used to develop an NIRS calibration predicting WSC concentrations with 90% accuracy. An interaction of species, nitrogen treatment, time of day, and harvest date influenced WSC concentrations in 10 harvests (P = .040). A modest positive relationship was observed between photosynthetically active radiation and WSC concentration when morning and afternoon samples were included (r = 0.503; P = .024). On nine harvest dates, perennial ryegrass or tall fescue were highest in WSC. High-WSC cultivars included “Aberzest” and “Calibra” perennial ryegrass, “Ginger” Kentucky bluegrass, and “Bronson” and “Cajun II” tall fescue. Water-soluble carbohydrates did not exceed 150 g/kg freeze-dried weight, possibly due to assay method, sampling times, or defoliation. The results suggest that minimizing WSC intake for horses may be possible by cultivar choice, grazing time, or mowing frequency.     

Endophyte-Free Tall Fescue Potential for Pastures on Fertile Clay Loam Soils in Central Louisiana

Although endophyte-free tall fescue (Festuca arundinacea Schreb.) cultivars have been recognized as lacking persistence on droughty, infertile, sandy Coastal Plain soils, they have continued to be recommended for the more fertile and heavier bottomland soils in Louisiana. Two pasture experiments were conducted to assess animal performance on, and persistence of, endophyte-free genotypes on the fertile soil of central Louisiana. In the first experiment, performance of young, growing, crossbred beef cattle on AU-Triumph and Forager tall fescue pastures was compared with that on Marshall annual ryegrass (Lolium multiflorum Lam.) using put-and-take stocking over three growing seasons. In the second experiment, year-round stocking and stocking only in the cool season, with and without fall hay harvest, were compared with set-stocked AU-Triumph tall fescue pastures for 2 yr. Individual animal performance on annual ryegrass at 0.74 kg/d was greater than that on the two tall fescue cultivars, which averaged 0.57 kg/d and did not differ from each other. Although animal performance was affected by management of AU-Triumph tall fescue in the first year of grazing, responses in the second year did not differ. Only 2 or 3 yr of productive grazing were obtained from endophyte-free tall fescue in these experiments on central Louisiana clay loam soil. Disease damage from Rhizoctonia solani Kuehn. contributed to stand loss and appeared to hasten deterioration from summer stress and invasion by aggressive warm-season grasses. Results of these experiments and reports from other locations in Louisiana indicate that endophyte-free tall fescue is not sufficiently adapted for use as a pasture plant, even on the more productive Louisiana soils.     

Horse Preference,Forage Yield,and Species Persistence of 12 Perennial Cool-Season Grass Mixtures Under Horse Grazing

Cool-season grass mixtures are rarely evaluated for preference, yield, and persistence under horse grazing. The objectives of this research were to evaluate horse preference, forage yield, and persistence of cool-season grass mixtures under horse grazing. Eight commercially marketed and four experimental perennial cool-season grass mixtures were planted in 2009 in a randomized complete block with five replicates and grazed by four adult horses during 2010, 2011, and 2012. All mixtures contained four to six cool-season perennial grass species. Specie density measurements were taken in each spring and fall, and yield was mechanically measured before each grazing period. After grazing, preference was determined by visually assessing percentage of forage removal on a scale of 0 (no grazing) to 100 (100% of vegetation removed). Data were analyzed using a mixed-model analysis of variance and liner regression. Horses preferred mixtures containing tall fescue, perennial ryegrass, Kentucky bluegrass, and timothy (P < .001). Horses had less preference for mixtures containing ≥30% orchardgrass (P < .001). Mixtures had similar (P = .11) forage yields that ranged from 6,100 to 7,082 kg ha⁻¹. After 2 years of grazing, orchardgrass and tall fescue increased; Kentucky bluegrass remained stable; and festulolium, meadow fescue, and perennial ryegrass had the greatest rate of decline in mixtures. Orchardgrass became the dominate species, regardless of initial percentage in the mixture. Mixtures containing tall fescue, perennial ryegrass, Kentucky bluegrass, and timothy should be planted in midwestern US horse pastures; however, mixtures will likely transition to tall fescue and Kentucky bluegrass–dominated pastures.     

Effect of level of endophyte infection, nitrogen fertilization rate, grazing period, and paddock exchange on some chemical properties of four bovine tissues.

Three grazing experiments were conducted to determine the effect of level of endophyte infection, rate of N fertilization of tall fescue grass, grazing period, and paddock exchange on selected chemical properties of four bovine carcass tissues. Samples of semitendinosus and longissimus muscle and of subcutaneous and perinephric adipose tissue were excised from the left side of each carcass. In Exp. 1, percentage of stearic acid was higher (P less than .05) and percentages of palmitoleic and oleic acid were lower (P less than .05) in all tissues from steers grazed on LELN Au-Triumph fescue than in tissues from steers grazed on LELN KY-31 fescue. Grazing periods of 175 or 245 d or paddock exchange (Exp. 2) had no significant effect on proximate composition of semitendinosus and longissimus muscles; however, moisture content was higher (P less than .05) in the semitendinosus muscle from steers grazed on 100% (100E) endophyte-infected KY-31 fescue. Forage treatment, grazing period, and paddock exchange (Exp. 2 and 3) had no significant effect on percentage of saturated fatty acids in the subcutaneous, semitendinosus, and longissimus tissue samples. In Exp. 2 and 3, percentages of saturated fatty acids were higher (P less than .05) in subcutaneous and perinephric adipose tissue samples from steers grazed on 100E than in samples from steers grazed on zero endophyte (OE) fescue.(ABSTRACT TRUNCATED AT 250 WORDS)     

Long-Term Effects of Aeration and Fire on Invasion of Exotic Grasses in Mixed-Brush Plant Communities

Invasion of exotic grasses into grasslands dominated by native plants changes fire cycles and reduces biodiversity. Brush management practices that create soil disturbance, such as aeration, may potentially result in invasion of exotic grasses and replacement of native vegetation. We tested the hypothesis that a long-term effect of aeration and prescribed burning is an increase in exotic grasses. The study was conducted at the Chaparral Wildlife Management Area in the western south Texas plains where four treatments were evaluated: aeration, warm-season burn, aeration followed by a warm-season burn, and no treatment (control). The experimental design was a randomized complete block with four replicates. We estimated percentage canopy cover of exotic grasses, native grasses, forbs, litter, bare ground, and woody and succulent plants in 2007. There was a multivariate main effect among treatments for the dependent variables absolute canopy cover of exotic grasses, native grasses, forbs, litter, and bare ground (Wilks's Lambda F_15,179.84 = 2.78, P = 0.001). Variables that contributed to the significant overall effect included litter (F_3,69 = 4.32, P = 0.008) and native grasses (F_3,69 = 6.11, P = 0.001). The multivariate main effect of treatment was significant (Wilks's Lambda F_9,180.25 = 2.04, P = 0.038) for the relative canopy cover of herbaceous species. Relative cover of exotic grasses was 31% higher (P = 0.024) in control than in the prescribed burn treatment. Native grasses relative cover was 30% higher (P = 0.003) in prescribed burn than in the control treatment. We did not detect differences among treatments in the percentage of total woody and succulent plants canopy cover (P = 0.083). Under the environmental conditions at the time of the study, aeration and/or prescribed burning do not increase exotic grasses.     

Forage systems for beef production from conception to slaughter: II. Stocker systems.

Fall weaned Angus calves grazed stockpiled 1) tall fescue (Festuca arundinacea Schreb.), 2) tall fescue-red clover (Trifolium pratense L.), or 3) tall fescue-alfalfa (Medicago sativa L.) or were barn-fed, 4) tall fescue hay, 5) orchardgrass (Dactylis glomerata L.)-alfalfa hay, or 6) tall fescue silage from late October to early April during each of 5 yr. Infection of the fescue with Acremonium coenophialum ranged from 0 to 55%. There were two replications each of steers and heifers for each forage system in a completely random design. Each replicate was grazed by three Angus stockers, except for System 1, which was grazed by six stockers, for a total of 420 stockers. Each pasture replicate contained .8 ha (except System 1, which was 1.6 ha), and the stocking rate was one stocker per .27 ha. Fescue hay and silage were harvested each spring for barn-fed systems from the area stockpiled for grazing by cattle in System 1. Nitrogen fertilizer (90 kg/ha) was applied in early spring and again in early August, before stockpiling; no N was applied to stockpiled fescue grown with legumes. Daily gains by calves grazing stockpiled fescue-alfalfa were greater (P less than .01) than by calves grazing stockpiled fescue-red clover or N-fertilized stockpiled fescue (.50, .33, and .34 kg/d, respectively), but fescue-alfalfa calves required more days (P less than .01) of supplemental hay feeding (105, 60, and 36, respectively). Calves fed fescue hay in the barn gained more (P less than .01) than those fed fescue silage.(ABSTRACT TRUNCATED AT 250 WORDS)     

Animal performance and economic comparison of novel and toxic endophyte tall fescues to cool-season annuals

Increased costs of annual establishment of small grain pasture associated with fuel, machinery, and labor are eroding the profitability of stocker cattle enterprises. Interest has therefore increased in development of cool-season perennial grasses that are persistent and high quality. This study occurred on 24 ha (divided into thirty 0.81-ha paddocks) located at the University of Arkansas Division of Agriculture Livestock and Forestry Branch Station, near Batesville. Two tall fescue (Festuca arundinacea Schreb.) cultivars infected with novel endophytes (NE), Jesup infected with AR542 endophyte (Jesup AR542), and HiMag infected with Number 11 endophyte (HM11) were established in September 2002. Jesup AR542 and HM11 were compared with endemic endophyte Kentucky 31 (KY-31) tall fescue; wheat (Triticum aestivum L.) and cereal rye (WR, Secale cereale L.) planted in September 2003, 2004, and 2005; and annual ryegrass [RG, Lolium perenne L. ssp. multiflorum (Lam.) Husnot] planted in September 2004 and 2005. Each year, 3 steers (3.7 steers/ha) were placed on each pasture for fall and winter grazing, and 5 steers (6.2 steers/ha) were placed on each pasture for spring grazing. Animal performance is presented by year in the presence of a year x treatment interaction (P < 0.01). Body weight gain per hectare of steers grazing NE tall fescue was greater (P < 0.01) than those of KY-31 and WR during 2003 to 2004, whereas in 2004 to 2005, BW gain per hectare of steers grazing NE and RG did not differ (P < or = 0.29) and was greater (P < 0.01) than that of WR, which was greater (P < 0.01) than that of KY-31. During 2005 to 2006, BW gain per hectare was greater (P < 0.01) for steers grazing RG than those of NE and WR, which did not differ (P > or = 0.14). Body weight gain per hectare was least (P < 0.01) for steers grazing KY-31. Average net return of NE tall fescue was greater (P < 0.01) than KY-31, but profitability of NE did not consistently differ from cool-season annuals. Across the 3-yr study, NE tall fescue produced net returns per hectare of $219; this level of profitability would require 4 yr for a new planting of NE tall fescue to break even. Novel endophyte tall fescues offer potential benefits related to decreased risk of stand establishment of annual forage crops, longer growing season, and acceptable animal performance.     

Establishing Wyoming Big Sagebrush in Annual Brome-Invaded Landscapes with Seeding and Herbicides

Seeding native plants into degraded grasslands presents major challenges. Often, seeded species fail to establish and areas become/remain dominated by unwanted plants. We combined herbicides and seeding in former coal mining fields dominated by exotic winter annual grasses (downy brome [Bromus tectorum L.] and Japanese brome [Bromus arvensis L.], hereafter “annual bromes”). The main interest was restoring Wyoming big sagebrush (Artemisia tridentata spp. wyomingensis [Beetle & A. Young] S.L. Welsh, hereafter “big sage”), a very difficult species to restore to North American grasslands. We tested the nonselective herbicide glyphosate and the grass-specific herbicide quizalofop. The summer following herbicide applications and seeding, annual brome cover in controls 22% (CI_95% 13%, 36%) was significantly greater (P < 0.03) than in glyphosate 11% (CI_95% 5%, 25%) and quizalofop 16% (CI_95% 7%, 35%) treatments. At Decker mine, glyphosate increased seeded big sage density (P < 0.04) from 0.76 (CI_95% 0.27, 2.11) to 3.05 (CI_95% 1.42, 6.56) plants ? m^-2 the second summer after seeding. Corresponding increases for Spring Creek mine were from 0.11 (CI_95% 0.03, 0.43) to 0.43 (CI_95% 0.13, 1.40) plants ? m^-2 (P < 0.04). These results were consistent across two experiments initiated in different years. In addition to big sage, our study’s seed mixes contained native grasses and forbs, and herbicide treatments tended to promote establishment of these plant groups. In annual brome-dominated areas of the northern Great Plains, conditions amenable to big sage seedling establishment do not appear entirely uncommon, and herbicides can increase establishment.     

Stocking Rate Effects on Steer Performance for Two Methods of Alleviating Fescue Toxicosis

Generally, grazing endophyte-infested tall fescue (Festuca arundinace Schreb.) in the late spring and summer is not recommended because of the effects of fescue toxicosis on cattle weight gains, which can be extreme. For steers conditioned to graze tall fescue in the early spring, stocking rate (3, 4, 5, and 6 steers/ha) effects were evaluated for two methods designed to avoid poor cattle performance during the late spring and summer (compensation period). The evaluation was conducted in 1997 and 1998. During the compensation phase, one replicate of each stocking rate was randomly assigned to 1-ha pastures of eastern gamagrass (Tripsacum dactyloides L.), and the other replicate of each stocking rate remained on tall fescue; the steers were fed a 1:1 mixture of broiler litter and corn at 2.27 kg as fed/steer per d. Average daily gain for steers fed eastern gamagrass during the compensation phase declined linearly (P<0.05) as stocking rate increased, but ADG for steers fed tall fescue plus the broiler litter-corn mixture tended to increase (P<0.10) as stocking rate increased. As a result, ADG and live weight gain (kilograms per hectare) with heavier stocking rates were higher for steers fed tall fescue plus the broiler litter-corn mixture, whereas the responses at a lighter stocking rate were higher for steers fed eastern gamagrass. At the conclusion of the compensation phase in 1998, steers fed tall fescue plus the broiler litter-corn mixture had lower (P<0.05) serum prolactin levels, and a higher (P<0.05) proportion of steers fed tall fescue had rough hair coats compared with those fed eastern gamagrass. Results of this study show that, for steers grazing tall fescue pastures, either eastern gamagrass or supplementation with a broiler litter-corn mixture can provide acceptable performance, but responses are affected by grazing pressure. Symptoms of fescue toxicosis can still occur, however.     

Behavior of steers grazing monocultures and binary mixtures of alfalfa and tall fescue.

Spectral analysis was used to relate dietary quality and herbage species to the behavior of grazing steers. Four .3-ha paddocks were established with either 'AU-Triumph' tall fescue (F; Festuca arundinacea Schreb.), 'Apollo' alfalfa (A; Medicago sativa L.), 1/3 fescue and 2/3 alfalfa (2/3A), or 2/3 fescue and 1/3 alfalfa (1/3A). Each paddock was stocked with 10 to 16 steers and defoliated in 5 d. Three steers on each paddock carried vibracorders to monitor grazing time. Daily forage samples were taken in 10-cm layers and weighed. Esophageal extrusa were collected from fistulated steers to measure diet quality. Daily grazing time did not differ (P = .37) among treatments; however, steers grazing mixtures grazed numerically longer (1.4 h/d) than steers on monocultures. Spectral analysis revealed that steers grazing A and 2/3A had many daily meals of short duration, but steers grazing 1/3A and F consumed three meals daily at 8-h intervals. Throughout the 4.67-d grazing period, quality of the diet linearly declined in crude protein and herbage digestibility, linearly increased in neutral detergent fiber and cellulose, and exhibited quadratic changes in lignin and ash. For most quality values, the tall fescue monoculture differed from the others (P < .05). Steers selected diets with similar quality for the A, 2/3A, and 1/3A treatments. This study illustrates how differences in forage diets alter grazing behavior of steers.     

Forage and breed effects on behavior and temperament of pregnant beef heifers

Background

Integration of behavioral observations with traditional selection schemes may lead to enhanced animal well-being and more profitable forage-based cattle production systems. Brahman-influenced (BR; n = 64) and Gelbvieh × Angus (GA; n = 64) heifers consumed either toxic endophyte-infected tall fescue (E+) or one of two nontoxic endophyte-infected tall fescue (NT) cultivars during two yr. Heifers were weighed at midpoint and termination of grazing. Grazing behavior (grazing, resting in the shade, lying, or standing without grazing) was recorded (n = 13 visual observations per yr in June and July) for each pasture. During yr 2, exit velocity (EV) and serum prolactin (PRL) were determined.

Results

Grazing behavior was influenced (P < 0.05) by an interaction between fescue cultivar and breed type. Gelbvieh × Angus heifers assigned to E+ pastures had the lowest percentage of animals grazing and the largest percentage of animals resting in the shade. Brahman-influenced heifers had faster EV (P < 0.001) than GA heifers (0.52 vs. 0.74 ± 0.04 s/m, respectively). Body weight (BW) was affected (P < 0.01) by an interaction of tall fescue cultivar and d, and an interaction of tall fescue cultivar and breed type. Heifers grazing NT pastures were heavier (P < 0.01) than heifers grazing E+ pastures at midpoint and termination. Gelbvieh × Angus heifers grazing NT pastures were heavier (P < 0.01) than GA and BR heifers grazing E+ and BR heifers grazing NT pastures. An interaction of forage cultivar and breed type occurred on serum PRL (P < 0.01).

Conclusion

Collectively fescue cultivar, EV, and concentrations of serum PRL were associated with grazing behavior. Heifers grazing NT pastures were observed to be grazing more than heifers assigned to E+ pastures, regardless of breed type, which may have contributed to changes in BW and average daily gain (ADG) in heifers. Integration of behavioral observations along with traditional selection schemes may lead to enhanced animal well-being and more profitable forage-based cattle production systems.     

Use of nonergot alkaloid-producing endophytes for alleviating tall fescue toxicosis in stocker cattle

Grazing studies were conducted to determine cattle growth performance, evaluate toxicosis, and compare grazing behavior in stocker cattle grazing nonergot alkaloid-producing endophyte-infected (AR542 or AR502), endophyte-free (E-), or wild-type toxic endophyte-infected (E+) Jesup, Georgia-5, and Kentucky-31 tall fescue. Replicated 0.81-ha tall fescue paddocks were established at the Central Georgia Branch Station at Eatonton and the Northwest Georgia Branch Station at Calhoun during October 1998 and were stocked with beef cattle for autumn and spring periods from fall 1999 through spring 2002. Mean ergot alkaloid concentrations were higher (P < 0.01) on E+ pastures than the other treatments at both locations. At Calhoun and Eatonton, post-treatment serum prolactin concentrations were decreased (P < 0.01) on E+ compared with AR542, AR502, and E- tall fescue. Cattle on AR542, AR502, and E- pastures had lower (P < 0.05) post-treatment rectal temperatures than cattle grazing E+ tall fescue during spring at Eatonton and Calhoun. Calf ADG was higher (P < 0.05) on AR542, AR502, and E- as compared with E+ tall fescue during autumn and spring grazing at Eatonton, and at Calhoun, cattle on E+ pastures had lower (P < 0.05) ADG in both autumn and spring. Gain/hectare was higher (P < 0.05) on AR542, AR502, and E- than on E+ during autumn at Eatonton and during spring at both locations. In autumn at Calhoun, gain/hectare was greater (P < 0.05) on AR502 and E- compared with E+ tall fescue. During April, May, and June, cattle grazing E+ pastures at Eatonton spent more (P < 0.01) time idling, more (P < 0.01) time standing, and used more (P < 0.01) water than cattle on AR542 and E- tall fescue. Daily prehensions and biting rate were each higher (P < 0.01) on AR542 and E- tall fescue than E+ tall fescue in both grazing seasons. There were no differences among pasture treatments for bite size in either spring (P = 0.50) or autumn (P = 0.34). Steers grazing E+ pastures had lower DMI than steers grazing AR542 and E- pastures during spring (P < 0.10) and lower DMI than steers grazing E- pastures during autumn (P < 0.05). Daily steer water usage was decreased (P < 0.10) in E+ pastures compared with AR542 and E- pastures during late fall. These results indicate that nonergot alkaloid-producing endophyte technology is a promising option for alleviating tall fescue toxicosis in stocker cattle.     

带内生真菌的野大麦草粉对3种草坪草种子萌发及生长的影响

以多年生黑麦草(Lolium perenne)、高羊茅(Festuca arundinacea)和草地早熟禾(Poa pratensis)为研究对象,用种子萌发法和盆栽法测定了野大麦(Hordeum brevisubulatum)内生真菌(Neotyphodium)对这3种草坪草种子萌发及生长的影响。结果表明,在种子萌发试验中,野大麦草粉对3种草坪草的生长均有一定的抑制作用,而且内生真菌显著增强了野大麦草粉对多年生黑麦草苗长、须根数和对草地早熟禾苗长的抑制作用（P<0.05）;显著降低了野大麦草粉对多年生黑麦草和高羊茅的发芽率及高羊茅苗长的抑制作用（P<0.05）。在盆栽试验中,野大麦草粉对3种草坪草的生长均有一定的促进作用,而且内生真菌显著增强了野大麦草粉对高羊茅和草地早熟禾的苗长和发芽率的促进作用（P<0.05）。     

丝茅侵入量对3种冷季型草坪草竞争力及生长潜力影响的差异

通过复合取代试验设计,设置5个侵入梯度,模拟丝茅入侵高羊茅、草地早熟禾、多年生黑麦草3种冷季型草坪幼坪,测定草坪草单株生物量、相对产量、相对产量总和、攻击力和竞争力及株高、分蘖数、茎基和根系性状等指标,研究侵入量对3种草坪草竞争力及生长潜力影响的差异。结果表明,1)丝茅与草坪草为拮抗关系,3种草坪草相对产量、相对产量总和极显著受丝茅入侵的影响(P<0.01),随侵入量增加草坪草的攻击力指数和种间竞争力不断下降,草种是影响草坪草攻击力和竞争力的主要因子,丝茅侵入量次之,受影响顺序为高羊茅>早熟禾>黑麦草;2)丝茅草侵入对3种草坪草分蘖数、茎基性状和根系性状均有显著影响,对分生再生能力的影响顺序为早熟禾>黑麦草>高羊茅,对根长和根生物量影响顺序为黑麦草>早熟禾>高羊茅;3)幼苗期黑麦草的现实攻击力和竞争力显著大于高羊茅和早熟禾(P<0.05),丝茅入侵对黑麦草根系胁迫度显著大于高羊茅和早熟禾(P<0.05),致使其生长潜力降低;高羊茅竞争力和攻击力虽低,而茎基和根系性状受丝茅入侵影响显著低于黑麦草和早熟禾,保持了极高的潜在生长力;4)丝茅入侵对3种草坪草竞争力和生长潜力均有影响,必将导致草坪种群组成与结构的破坏。所以,加强草坪养护、防治丝茅入侵,是提高草坪品质、延长草坪寿命的根本途径。     

利用禾草隔离技术防控鼢鼠危害豆科人工草地的研究

豆科牧草来源匮乏始终是制约高寒牧区畜牧业发展的短板之一。除了天然草地资源本身的限制以外,豆科牧草人工草地的建植也存在诸多限制因素,其中一个重要的限制因素就是鼢鼠(Myospalax)危害。研究表明,鼢鼠对豆科等轴根系植物具有强烈的采食偏向性,而对禾本科等须根系植物则表现为相对冷淡。据此,本研究采用在豆科草地外围种植禾本科草隔离带的方式,从草种选择、隔离带宽度、种植方式等技术环节对此种方法的有效性和技术要点进行了探索。结果表明:禾草隔离技术是防控豆科人工草地鼢鼠危害的有效方法,平均可减少危害量82.4%以上;一年生禾草的隔离效果明显优于多年生禾草(P0.01),短期多年生禾草明显优于长期多年生禾草(P0.01);隔离带宽度应控制在3~5m之间;豆科草地建植当年以一年生、短期多年生和长期多年生混播隔离带效果最佳。     

几种草坪草在海南地区的适应性研究

对暖季型草一般百慕大、撒哈拉百慕大、日本结缕草；冷季型草哥伦比亚早熟禾、白三叶、高羊茅进行了生物学特性观察、年生长速率变化的研究，结果表明，暖季型草都很适应海南地区的气候条件，３￣１０月是生长旺盛期，元月低温期间受轻微冻伤，叶片出现紫斑沉淀，但仍可全年保持绿色，冷季型草当年１１月至次年４月生长旺盛，５￣１０月生长逐渐减慢以至停止、死亡。     

Reproductive characteristics of endophyte-infected or novel tall fescue fed ewes

Reduced pregnancy rates often occur in ruminants grazing endophyte-infected (EI) tall fescue. The objectives were to characterize basal and oxytocin-induced PGF_2α concentrations in serum and reproductive function in ewes fed tall fescue seed and to determine whether addition of fish meal (FM) to a diet of EI fescue would alter PGF_2α production. Ewes were fed a diet with novel or non-toxic endophyte-infected (NE) or EI tall fescue seed containing either corn gluten meal (CG) or FM. Serum concentrations of prolactin, a measure of severity of fescue toxins, were reduced in ewes fed EI compared with NE forage seed (forage × day, P < 0.02) and were greater in NEFM than NECG-fed ewes (P < 0.03). Size and number of corpora lutea (CL), determined by trans-rectal ultrasonography, were similar between diets (P > 0.10). Serum concentrations of progesterone were reduced in ewes with two CL fed EI compared with NE seed (forage × CL number × day, P < 0.001). Oxytocin-induced PGFM concentrations during the luteal phase were determined as a measure of uterine function. On the day of oxytocin administration, peak plasma concentrations of PGFM were reduced in EI compared with NE-fed ewes (forage × time, P < 0.003), but FM did not influence PGFM concentrations. Estrous cycle length was more variable in EI than NE-fed ewes. There appears to be some asynchrony between NE and EI-fed ewes leading to changes in uterine responsiveness to oxytocin. Inclusion of FM did not alter uterine responsiveness to oxytocin.