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甜樱桃矮化砧木吉塞拉(Gisela)的离体叶片再生植株研究 总被引:16,自引:2,他引:16
用甜樱桃矮化砧木吉塞拉(Gisela)5号、7号(Prunus cerasus x P.canescens)无菌生根苗的叶片作为外植体,进行不定芽再生植株的研究。以WPM+BA 5~7 mg/L+IBA 0.1~1.0mg/L做培养基,不定芽再生率高达70%;吉塞拉5号的再生率明显高于7号;培养基中高浓度的细胞分裂素抑制吉塞拉7号的不定芽再生。不定芽的增殖、生根、温室锻炼、大田移栽均已获得成功,同时观察了植株在大田的生长状况。该成果可用于樱桃转基因育种和多倍体植株培育的研究。 相似文献
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吉塞拉6号甜樱桃矮化砧木由德国育成。其植物学性状特点等同于灰毛叶樱桃,为酸樱桃与灰毛叶樱桃进行种间杂交培育的三倍体杂种,在欧洲北美广泛应用。该砧木1998年从美国引入我国.经7a(年)的区域试验和生产试验观察,与大多数甜樱桃品种亲合性良好,具有明显的矮化、丰产、早实性,抗病、耐涝、土壤适应范围广,固地性能好,抗寒,产量、效益高。2004年12月通过国家林业局林木良种审定委员会审定。 相似文献
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砧木是限制甜樱桃生产的关键问题之一。‘吉塞拉6号’,甜樱桃矮化砧木由德国育成.其植物学性状特点等同于灰毛叶樱桃.为酸樱桃与灰毛叶樱桃进行种间杂交培育的三倍体杂种.在欧洲、北美广泛应用。该种砧木1998年经美国引入我国.经7年的区域试验和生产试验观察.与大多数甜樱桃品种亲合性良好,具有明显的矮化、丰产、旱实性强、抗病、耐涝、土壤适应范围广、固地性能好、抗寒、产量效率高等优点.2004年12月通过国家林业局林木良种审定委员会审定(国S—SV—PCP-015-2004)。 相似文献
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莫利甜樱桃试管快繁技术研究 总被引:4,自引:0,他引:4
利用莫利甜樱桃的茎段做外植体进行试管快繁 ,适宜的增殖培养基为MS BA 0 .8mg/L IAA0 .2mg/L ,生根培养基为 1 / 2MS NAA 0 .5mg/L ,增殖系数可达 1 0倍左右 ,生根率达 92 .5 % ,选 5片以上有效叶的健壮苗移入蛭石基质 ,移栽成活率可达 95 %以上。 相似文献
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为探索低温胁迫对甜樱桃子房和花柱AsA-GSH循环的影响,以及外源ALA对低温伤害的缓解机理,以甜樱桃‘红玛瑙’为试材,分析了不同浓度ALA对低温胁迫下甜樱桃子房和花柱AsA-GSH循环的调控作用。结果表明,喷施适量的ALA可有效降低低温胁迫下甜樱桃子房和花柱中超氧阴离子自由基(O2·-)和过氧化氢(H2O2)的积累,提高还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量,降低氧化型抗坏血酸(DHA)和氧化型谷胱甘肽(GSSG)含量,提高AsA-GSH循环中相关酶的活性。其中以浓度为50 mg/L的ALA作用效果最好,75 mg/L的ALA作用效果次之。为进一步明确ALA缓解甜樱桃花器官低温伤害的作用机制提供了理论依据。 相似文献
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温度对休眠期甜樱桃芽呼吸代谢影响的研究 总被引:3,自引:0,他引:3
以5年生甜樱桃“极佳”为试材,测定了自然休眠期花芽和叶芽的呼吸强度和呼吸途径的变化,并对不同温度处理(5℃、20℃和变温5/20℃)的花芽和叶芽的呼吸强度和呼吸途径进行了测定。结果表明:低温来临前樱桃芽呼吸强度高,低温来临后呼吸强度大幅度下降并维持较低的代谢水平,休眠末期,呼吸强度开始缓慢上升;体眠期3条主要呼吸途径发生显著变化:糖酵解途径(EMP)呈现下降趋势,三羧酸循环途径(TCA)变化不大,并占优势,磷酸戊糖途径(PPP)则呈明显上升趋势。高温对呼吸强度具有促进作用,而低温对樱桃芽呼吸强度有先抑制后促进的作用。与对照相比,高温提高了EMP的比率,降低了PPP的比率;而低温则使PPP不断活化,这可能是低温可以缩短自然休眠进程的原因之一。 相似文献
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Lan Shing Hwang Robert M. Skirvin Jean Casyao John Bouwkamp 《Scientia Horticulturae》1983,20(2):119-129
Sweet potato (Ipomoea batatas Lam.) root sections were obtained from the cultivars ‘Centennial’, ‘Redmar’ and ‘Jewel’ with a No. 6 cork borer. These sections were cut into 2–3-mm discs and explanted on to modified Murashige and Skoog (MS) medium consisting of MS high mineral salts, myo-inositol (100 mg l?1), Staba vitamins, 6-benzyl-aminopurine (2.0 mg l?1), naphthaleneacetic acid (0.1 mg l?1), sucrose (30 g l?1) and agar (10 g l?1). Root discs from internal regions of the tuberous roots gave rise to calli and meristematic bud-like centers (MBLC's). A small percentage of ‘Centennial’ MBLC's burst open to reveal plantlets which grew and rooted well on the medium. Some of the ‘Jewel’ MBLC's contained only roots, while those of ‘Redmar’ did not differentiate. MBLC-formation occurred most often on discs taken from fresh (unstored) roots of ‘Centennial’. Petiole sections taken from in vitro-cultured plants of all 3 cultivars developed plants quite readily on the medium. Shoots of all 3 cultivars grew rapidly, to yield whole rooted plants which could easily be moved to soil and grown in the greenhouse and field. 相似文献
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‘St. Julien A’ (Prunus instititia L.) rootstock was induced to proliferate shoots on a modified half-strength Murashige and Skoog (MS) medium. Cultures treated with 12.5 mg l?1 gibberellic acid (GA3) produced elongated shoots suitable for rooting. Elongated shoots were placed in media with indolebutyric acid (IBA) or indole-3-acetic acid (IAA) with or without a 16-day dark incubation. Light (16-h photoperiod) inhibited rooting. IAA (4 mg l?1) was ineffective in promoting rooting. Rooting was best when shoots were incubated in the dark with IBA (4 mg l?1). GA3 was deleterious to shoots, causing chlorosis and apical die-back. Light regime interacted with auxin treatments in affecting shoot condition. Shoot condition was better on shoots treated with IBA and dark-incubated; while those treated with IAA were better when light-incubated. 相似文献