The microbial biomass in paleosols buried under kurgans and in recent soils in the steppe zone of the Lower Volga region

The total microbial biomass (TMB) was assessed in the chestnut and light chestnut soils and in the paleosols under burial mounds (steppe kurgans) in the Lower Volga region on the basis of data on the organic carbon content in the extracted microbial fraction supplemented with the data on the extraction completeness as a conversion coefficient. The completeness of the microbial fraction extraction was determined by direct counting of the microbial cells and colony-forming units (on plates with soil agar). The total microbial biomass varied from 400 to 6600 μg of C/soil. Its values in the buried soils were 3–5 times lower than those in the surface soils. The TMB distribution in the buried chestnut soil profile was close to that in its modern analogue (with the minimum in the B1 horizon). In the buried light chestnut paleosols, the TMB values usually increased down the profile; in the recent light chestnut soils, the maximum TMB values were found in the uppermost horizon.     

Comparative characterization of microbial communities in kurgans,paleosols buried under them,and background surface soils in the steppe zone of the Lower Volga region

A comparative analysis of the state of microbial communities in kurgans, paleosols buried under them, and background surface soils in the dry steppe zone of the Lower Volga region has been performed. It is shown that the population density of microorganisms of various trophic groups in the kurgans is an order of magnitude lower than that in the A1 horizon of the corresponding buried paleosols and background surface soils within the areas of chestnut, light chestnut, and solonetzic soils. The respiration activity of the microbial communities in the upper layer of the kurgans is comparable with that in the A1 horizons of the background surface soils; it decreases in the deeper layers of the kurgans. In the A1 horizon of the buried paleosols, the respiration activity is approximately the same as in the deep layers of the kurgans. In the buried paleosols, the spatial variability in the numbers of soil microorganisms is approximately the same or somewhat higher than that in the background surface soils. The spatial variability in the respiration activity of the buried paleosols is two to four times higher than that in the background surface soils.     

The structural state of buried and surface soils of solonetzic complexes in the dry steppe zone of the Lower Volga basin

The structural state of modern (surface) soils and the soils buried under Anna Ioannovna??s rampart (1718?C1720) was studied. These soils are the components of solonetzic soil complexes in the southern Privolzhskaya Upland. The dehumification and the high content of calcium in the exchange complex determine the state of the macrostructure of the chestnut soil buried about 300 years ago. The dehumification drastically lowers the water stability of the soil aggregates, and the predominance of calcium ions in the soil exchange complex prevents the destruction of the chestnut paleosol aggregates and preserves their aggregate state upon moistening. For the last 300 years, no significant changes in the macrostructure of the solonetzes have been observed.     

The morphology of cells and the biomass of microorganisms in the buried paleosols and modern steppe soils of the Lower Volga region

The morphology of microbial cells was studied, and the biomass of microorganisms was estimated in the modern steppe soils and paleosols buried under kurgans in the Lower Volga region with the methods of electron microscopy. The shape and ultrastructure of the cells in the modern soils and paleosols were similar, though their average volumes differed (0.37 and 0.28 μm³, respectively). The portion of cells with a volume above 1 μm³ in the surface soils and paleosols reached 10.9 and 9.2%, respectively, and the portion of cells with a volume less than 0.01 μm³ in the surface soils was 10% lower than that in the buried paleosols. It was found that the cells of the microorganisms have an external organomineral layer, which increases the cell volume by 4.9 times, and this fact was taken into account in the calculation of the microbial biomass. In the chestnut and light chestnut paleosols, the latter comprised 1500 and 230 μg of C/g soil, respectively.     

Modern and buried soils of kurgans in the forest-steppe zone of the Middle Volga region (by the example of Komintern I kurgan)

The construction of the Volga–Kama cascade of water reservoirs and hydroelectric power stations in the middle of the 20th century resulted in the inundation of vast areas and the development of abrasion along the shores that threatens many monuments of the archaeological and cultural heritage. The soils buried under northernmost kurgans of the Lugovskaya culture dating back to the 15th–14th centuries BC (kurgan Komintern I) were studied on the surface of the second terrace of the Kama River near its confluence with the Volga River. Burial sites of kurgan Komintern III were subjected to destruction in 1981, and archaeological excavations of kurgan Komintern II were performed in 2008. The danger of complete disappearance of these archaeological sites necessitated their thorough study with the use of multiple methods, including special paleosol studies. The soils buried under the kurgans ceased to be active components of the soil cover about 3500 years ago. They preserve information on the paleoenvironmental conditions before their burying. The analysis of morphological features and physical, physicochemical, and chemical properties of the buried soils attests to their chernozemic nature. Background surface soils that have passed through the entire cycle of the Holocene pedogenesis have evolved since that time into gray forest soils (Luvic Greyzemic Phaeozems) under forest vegetation. These soils are characterized by the increased acidity of the surface horizons (pH_КСl 4.3) and the development of lessivage. Data on the coefficients of mineral weathering in the buried paleosols and background surface soils attest to the identical precipitation both for the surface and buried soils in the Middle and Late Holocene.     

Transformation of carbonate pedofeatures in paleosols buried under kurgans in the North Caucasus region

Carbonate concentrations in a chronosequence of paleosols buried under kurgans in the North Caucasus region at the end of the 4th century and the first half of the 5th century AD have been studied with the use of a set of morphological and isotopic methods. It is demonstrated that morphologically different phases of calcite—the collomorphic phase and the crystalline phase—in carbonate pedofeatures (calcareous pseudomycelium) and in the calcareous horizon have different elemental compositions and different isotopic compositions of carbon. Hence, these forms of calcite should have different origins. An addition of colloidal carbonates migrating in colloidal solutions from the lower soil horizons to the surface horizons during the periods of climatic aridization to the acicular calcite may be responsible for a sharp and irregular increase in the radiocarbon age of the newly formed carbonate pseudomycelium.     

Spatial variability of microbial communities in modern and buried soils of the Sakarka River Basin,the Privolzhskaya Upland

A comparative study of the microbial communities of the buried soils under the Anna Ioannovna mound (1718–1720) and the modern soils developed on different geomorphic positions (watershed, slope, and high floodplain) in the Sakarka River basin (the Privolzhskaya Upland) in the dry steppe zone was performed. The varying soil and ecological conditions of the different relief features were established to dictate the differences in the characteristics of the microbial community of the buried and modern soils, in particular, concerning the number of microorganisms belonging to different trophic groups (those restricted to sparse nutrients, humus-consuming ones, and those utilizing easily available organic matter), the ecologo-trophic structure, and the mycelia biomass and structure (the ratio between the light- and dark-colored hyphae). On the basis of the dispersion analysis, a qualitative estimation of the relief features’ impact on the spatial variations of the microbiological parameters of the soils under study is presented.     

Paleosols buried under kurgans of the Pit-grave culture in the steppe zone of the Cis-Ural region

The analysis of soil chronosequences developed from loamy and loamy sandy substrates and buried under kurgans dating back to the Pit-grave archaeological culture in the steppe zone of the Cis-Ural region demonstrated that it is possible to trace the dynamics of the soil properties and to perform paleoclimatic reconstructions for different intervals within the studied period. The properties of sandy soils changed more rapidly than the properties of loamy soils. For sandy soils, notable changes in their properties were revealed in the soil chronosequences dating back to the particular stages of the Pit-grave culture; for loamy soils, such changes could only be traced for the soils buried under the kurgans dating back to different stages of the Pit-grave culture.     

Changes in the properties of solonetzic soil complexes in the dry steppe zone under anthropogenic impacts

Long-term studies of changes in the properties of solonetzic soil complexes of the dry steppe zone under anthropogenic impacts (deep plowing, surface leveling, irrigation, and post-irrigation use) have been performed on the Privolzhskaya sand ridge and the Khvalyn and Ergeni plains. The natural morphology of solonetzic soils was strongly disturbed during their deep ameliorative plowing. At present, the soil cover consists of solonetzic agrozems (Sodic Protosalic Cambisols (Loamic, Aric, Protocalcic)), textural (clay-illuvial) calcareous agrozems (Eutric Cambisols (Loamic, Aric, Protocalcic)), agrosolonetzes (Endocalcaric Luvisols (Loamic, Aric, Cutanic, Protosodic), agrochestnut soils (Eutric Cambisols (Siltic, Aric)), and meadowchestnut soils (Haplic Кastanozems). No features attesting to the restoration of the initial profile of solonetzes have been found. The dynamics of soluble salts and exchangeable sodium differ in the agrosolonetzes and solonetzic agrozems. A rise in pH values takes place in the middle part of the soil profiles on the Khvalyn and Ergeni plains.     

Microbial communities of alluvial soils in the Volga River delta

The number and biomass of the microbial community in the upper humus horizon (0–20 cm) were determined in the main types of alluvial soils (mucky gley, desertified soddy calcareous, hydrometamorphic dark-humus soils) in the Volga River delta. Fungal mycelium and alga cells predominate in the biomass of the microorganisms (35–50% and 30–47%, respectively). The proportion of prokaryotes in the microbial biomass of the alluvial soils amounts to 2–6%. No significant seasonal dynamics in the number and biomass of microorganisms were revealed in the alluvial soils. The share of carbon of the microbial biomass in the total carbon content of the soil organic matter is 1.4–2.3% in the spring. High coefficients of microbial mineralization and oligotrophy characterize the processes of organic matter decomposition in the alluvial soils of the mucky gley, desertified soddy calcareous, and hydrometamorphic dark humus soil types.     

Mineralogical and chemical compositions of the paleosols of different ages buried under kurgans in the southern Ergeni region and their paleoclimatic interpretation

The chemical and mineralogical compositions and the contents and properties of the organic matter were studied in the paleosols of different ages buried under the kurgan group “Kalmykia” in the southern part of the Ergeni Upland. The investigated sequence of soils included profiles developed on the given territory about 5100, 4410, 4260, 4120, 3960, and 600 yrs ago. The background light chestnut soil was also examined. The results of our study showed that the earlier established climate changes in this area during the second half of the Holocene are reflected in the chemical and mineralogical compositions of the soils. These characteristics can be used as indicators of the paleoclimatic conditions together with some petrophysical characteristics, such as the magnetic susceptibility of the soil samples. The study of the mineralogical composition of the clay fraction of the paleosols attests to the transformation of the smectitic phase, accumulation of illites, and destruction of chlorites manifested at different degrees. It is argued that the geochemical indices—CIA, Al₂O₃/(CaO + MgO + K₂O + Na₂O), Rb/Sr, and Ba/Sr—are sensitive to climate changes and reflect the transformation of the mineral soil mass and the soil genesis.     

The solonetzic process in surface soils and buried paleosols and its reflection in the mineralogical soil memory

The development of the solonetzic process in paleosols buried under kurgans and in the modern surface soils has been studied on the basis of the analysis of the clay (<1 μm) fraction. The revealed changes in the textural differentiation of the soils and the mineralogical composition of the clay fraction during 4500 years are assessed from the viewpoint of the “memory“ of the solid-phase soil components. The mineralogical characteristics show that the solonetzic process in the modern background soil is more developed. The mineralogical approach allows us to reveal the long-term changes in the soil status; it is less useful for studying the effect of short-term bioclimatic fluctuations. In the latter case, more labile soil characteristics should be used. The mineralogical method, combined with other methods, becomes more informative upon the study of soil chronosequences. Our studies have shown that the data on the clay minerals in the buried paleosols may contain specific information useful for paleoreconstructions that is not provided by other methods.     

Diversity of clay minerals in soils of solonetzic complexes in the southeast of Western Siberia

Data on the mineralogical composition of clay in soils of solonetzic complexes of the Priobskoe Plateau and the Kulunda and Baraba lowlands have been generalized. The parent materials predominating in these regions have loamy and clayey textures and are characterized by the association of clay minerals represented by dioctahedral and trioctahedral mica–hydromica, chlorite, kaolinite, and a number of irregular interstratifications. They differ in the proportions between the major mineral phases and in the qualitative composition of the minerals. Mica–hydromica and chlorites with a small amount of smectitic phase predominate on the Priobskoe Plateau and in the Kulunda Lowland; in the Baraba Lowland, the portion of mica–smectite interstratifications is higher. An eluvial–illuvial distribution of clay fraction in solonetzes is accompanied by the acid–alkaline destruction and lessivage of clay minerals, including the smectitic phase in the superdispersed state. This results in the strong transformation of the mineralogical composition of the upper (suprasolonetzic) horizons and in the enrichment of the solonetzic horizons with the products of mineral destruction; superdispersed smectite; and undestroyed particles of hydromica, kaolinite, and chlorite from the suprasolonetzic horizons. A significant decrease in the content of smectitic phase in the surface solodic horizons of solonetzic complexes has different consequences in the studied regions. In the soils of the Priobskoe Plateau and Kulunda Lowland with a relatively low content (10–30%) of smectitic phase represented by chlorite–smectite interstratifications, this phase virtually disappears from the soils (there are only rare cases of its preservation). In the soils of the Baraba Lowland developed from the parent materials with the high content (30–50%) of smectitic phase represented by mica–smectite interstratifications, the similar decrease (by 10–20%) in the content of smectitic phase does not result in its complete disappearance. However, the smectitic phase acquires the superdispersed state and the capacity for migration.     

Modern tendencies of changes in the properties of virgin and post-irrigational soils of solonetzic complexes on the Privolzhskaya ridge

The study of post-irrigational and virgin soils of solonetzic complexes on the Privolzhskaya sandy ridge has shown that the morphological features of paleohydromorphism and the features acquired during the 30-year-long irrigation period are well preserved in the soil profiles. After the cessation of irrigation, the upper boundary of effervescence has shifted closer to the soil’s surface; fine-crystalline gypsum has appeared in channel pores. When the groundwater level is deeper than 4 m, the boundary of effervescence is higher than the boundary of the gypsum concentrations. When the groundwater level is about 3 m, these two boundaries coincide. If the groundwater level is higher than 2.5 m, the boundary of the effervescence is found deeper than the boundary of the gypsum concentrations. Salinization and solonetzization of the post-irrigational soils have not been identified. In the virgin soils with the groundwater level of 2.3–3.0 m, the content of exchangeable sodium in the upper 30 cm is similar to that in the post-irrigational soils. In the deeper layers, the exchangeable sodium content in the solonetzes and chestnut soils rises up to 25% and more; in the meadow-chestnut soils, it constitutes up to 4% of the sum of exchangeable bases.     

Age and activation of microbial communities in soils under burial mounds and in recent surface soils of steppe zone

Chestnut paleosols buried under steppe kurgans about 4800, 4000, and 2000 years ago and their background analogues were studied in the dry steppe zone on the Volga-Don interfluve. Morphological, chemical, microbiological, biochemical, and radiocarbon studies were performed. Paleoclimatic conditions in the region were reconstructed on the basis of paleosol data. The ages of microbial fractions isolated from the buried and surface soils were determined using the method of ¹⁴C atomic mass-spectrometry. It reached 2100 years in the A1 horizon of the buried paleosol, which corresponded to the archaeological age of the kurgan (1st century AD). The ages of microbial biomass isolated from the B2 horizons of the buried paleosol and the background surface soil comprised 3680 ± 35 and 3300 ± 30 years, respectively. The obtained data confirmed our assumption about preservation of microorganisms of the past epochs in the paleosols buried under archaeological monuments. It is ensured by various mechanisms of adaptation of soil microbial communities to unfavorable environmental conditions (anabiosis, transformation of bacteria into nanoforms, etc.). The possibility to stimulate germination of the ancient dormant microbial pool isolated from the buried paleosols by 2–3 orders of magnitude with the use of β-indolyl-3-acetic acid as a signal substance was demonstrated.     

二甲基二硫熏蒸对保护地连作土壤微生物群落的影响

随着保护地高附加值经济作物的连年栽培, 土传病害问题愈发突出, 熏蒸剂也因此得以更广泛的应用。但鉴于熏蒸剂的广谱性, 在杀死有害生物的同时, 不可避免地对非靶标生物产生一定的影响。为明确溴甲烷替代药剂二甲基二硫(dimethyl disulfide, 简称DMDS)熏蒸对土壤微生物群落的影响, 本研究在室内条件下采用BIOLOG 方法, 测定不同浓度DMDS 熏蒸对保护地连作土壤微生物群落的影响。研究结果表明: 不同浓度DMDS(170.00 mg·kg^-1、85.20 mg·kg^-1、42.50 mg·kg^-1、21.30 mg·kg^-1 和10.62 mg·kg^-1)熏蒸处理对镰孢菌属(Fusarium spp.)和疫霉菌属(Phytophthora spp.)的LC₅₀(抑制中浓度)分别为42.08 mg·kg^-1 和115.15 mg·kg^-1。DMDS 熏蒸后恢复培养0 d 取样, 温育120 h 时, 170.00 mg·kg^-1、42.50 mg·kg^-1 和10.62 mg·kg^-1 的DMDS 处理土壤的AWCD 值(平均每孔颜色变化率, average well-color development, AWCD)分别比空白对照升高8.46%、6.02%、19.31%, 表明DMDS 促进了土壤微生物的生长。恢复培养14 d 后, 各处理土壤微生物的AWCD 值恢复至对照水平。多样性指数分析显示, DMDS 熏蒸后恢复培养0 d 时, 土壤微生物群落的Shannon 指数、Simpson指数均高于空白对照, McIntosh 指数与对照无显著性差异; 恢复培养7 d 后, Shannon 指数与Simpson 指数恢复至对照水平。主成分分析结果显示, DMDS 熏蒸后恢复培养0 d 时, 各处理间微生物对碳源的利用方式差异显著, 恢复培养14 d 后, DMDS 对微生物碳源利用方式的影响逐渐减弱, 恢复至对照水平。结果表明, DMDS 熏蒸处理对土壤微生物的生长具有促进作用, 影响了微生物对碳源的利用方式, 但在恢复培养14 d 后, 被干扰的土壤微生物逐渐恢复至对照水平。DMDS 熏蒸处理在有效防控土传病原真菌的同时, 不会对土壤微生物群落产生明显的扰动影响, 对环境较安全。     

On the manifestation of solonetzic properties in soils of the steppe and dry steppe zones in the south of Ukraine

Solonetzic soils are widespread in the south of Ukraine; some of them are represented by low-sodium solonetzes whose diagnostics is complicated by the absence of correspondence between the low content of exchangeable sodium and the clearly pronounced features of the illuvial solonetzic horizon. The statistical treatment of available literature, personal materials, and the materials of soil surveys in the south of Ukraine demonstrated that the diagnostics of low-sodium solonetzes should be based on the assessment of the degree of illuviation in the soil profile. It was shown that the degree of correlation between the content of exchangeable sodium and the degree of illuviation of the soil profile varies in dependence on the size of the statistical sample and differs for the low-sodium and medium-sodium solonetzes. Two scales characterizing the degree of soil illuviation were developed for these soil groups. It is suggested that these scales should be applied during soil surveys in the areas with solonetzic soils.     

Post-fire transformation of the microbial complexes in soils of larch forests in the lower Angara River region

The postfire transformation of the functional activity of the microbial cenoses and the main soil properties under mixed larch forests were studied in the lower reaches of the Angara River. It was shown that the intensity of the postfire changes in the population density, biomass, and activity of the microorganisms in the dark podzolized brown forest soil depended on the degree of burning of the ground cover and the surface litter during the fire. The maximum effects of the fire on the microbial cenoses were observed in the litter and the upper 5-cm-thick layer of the dark-humus horizon in the areas of intense burning. The postfire restoration of the structural-functional activity of the microbial cenoses was determined by the degree of transformation of soil properties and by the postpyrogenic succession in the ground cover. The microbial complexes of the dark podzolized brown forest soils under mixed larch forests in the studied region restored their functional activity after the fires of different intensities quicker than the microbial cenoses of the sandy podzols in the pyrogenic lichen-green-moss pine forests of the same zone.     

Comparison of two approaches for assessing solonetzic process in the virgin and human-transformed soils of the dry steppe zone

Two approaches to diagnose the development of secondary solonetzic process in the reclaimed solonetzes and solonetzic soils of the dry steppe zone were compared. For this purpose, the kinetics of swelling of crushed soil and the physicochemical characteristics of the soils were studied in the same samples. Both approaches gave similar results for the soils with the absence of the solonetzic process and for the soils in which this process was clearly manifested. To assess the risk of the development of the solonetzic process in the soils where its morphological manifestations are absent, the determination of the swelling kinetics and the calculation of the B index of the physicochemical conditions may be used.