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1.
Cucumber mosaic virus (CMV) was isolated from a mosaic diseased plant of Eucharis grandiflora. The virus caused mosaic symptoms on leaves and slight distortion of flower petals in E. grandiflora by either mechanical or aphid inoculation. The virus was identified as a strain of CMV subgroup I from its biological and serological characteristics. 相似文献
2.
Ramesh R. Chavan Michael N. Pearson Dan Cohen 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(2):247-259
Actinidia chinensis and A. deliciosa plants from China, showing a range of symptoms, including vein clearing, interveinal mottling, mosaics and chlorotic ring
spots, were found to contain ~300 nm rod-shaped virus particles. The virus was mechanically transmitted to several herbaceous
indicators causing systemic infections in Nicotiana benthamiana, N. clevelandii, and N. occidentalis, and local lesions in Chenopodium quinoa. Systemically- infected leaves reacted with a Tobacco mosaic virus polyclonal antibody in indirect ELISA. PCR using generic and specific Tobamovirus primers produced a 1,526 bp sequence spanning the coat protein (CP), movement protein (MP), and partial RNA replicase genes
which showed a maximum nucleotide identity (88%) with Turnip vein clearing virus and Penstemon ringspot virus. However, when the CP sequence alone was considered the highest CP sequence identity (96% nt and 98% aa) was to Ribgrass mosaic virus strain Kons 1105. The morphological, transmission, serological and molecular properties indicate that the virus is a member
of subgroup 3 of the genus Tobamovirus. 相似文献
3.
Mona Rabie Claudio Ratti Matteo Calassanzio Engy Abdel Aleem Faiza Aref Fattouh 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(1):219-225
Four Cucumber mosaic virus (CMV) (CMV-HM 1–4) and nine Tomato mosaic virus (ToMV) (ToMV AH 1–9) isolates detected in tomato samples collected from different governorates in Egypt during 2014, were here characterized. According to the coat protein gene sequence and to the complete nucleotide sequence of total genomic RNA1, RNA2 and RNA3 of CMV-HM3 the new Egyptian isolates are related to members of the CMV subgroup IB. The nine ToMV Egyptian isolates were characterized by sequence analysis of the coat protein and the movement protein genes. All isolates were grouped within the same branch and showed high relatedness to all considered isolates (98–99%). Complete nucleotide sequence of total genomic RNA of ToMV AH4 isolate was obtained and its comparison showed a closer degree of relatedness to isolate 99–1 from the USA (99%). To our knowledge, this is the first report of CMV isolates from subgroup IB in Egypt and the first full length sequencing of an ToMV Egyptian isolate. 相似文献
4.
Barley yellow dwarf disease is one of the most important problems confronting cereal production in Iran. Barley yellow dwarf virus-PAV (BYDV-PAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV) are the predominant viruses associated with the disease. One isolate of BYDV-PAV from wheat (PAV-IR) and one
isolate of CYDV-RPV from barley (RPV-IR) were selected for molecular characterisations. A genome segment of each isolate was
amplified by PCR. The PAV-IR fragment (1264 nt) covered a region containing partial genes for coat protein (CP), read through
protein (RTP) and movement protein (MP). PAV-IR showed a high sequence identity to PAV isolates from USA, France and Japan
(96–97%). In a phylogenetic analysis it was placed into PAV group I together with PAV isolates from barley and oats. The fragment
of RPV-IR (719 nt) contained partial genes for CP, RTP and MP. The sequence information confirmed its identity as CYDV. However,
RPV-IR showed 90–91% identity with both RPV and Cereal yellow dwarf virus-RPS (CYDV-RPS). Phylogenetic analyses suggested that it was more closely related to RPS. These data comprise the first attempt
to characterise BYD-causing viruses in Iran and southwest Asia.
The nucleotide sequence data reported appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession
numbers AY450425 and AY450454 相似文献
5.
Pymetrozine, a novel compound belonging to the class pyridine-azomethines, is a feeding inhibitor labeled for use against
plant pests in the order Hemiptera. Pymetrozine was evaluated for its ability to interfere with whitefly transmission of the
begomovirusTomato yellow leaf curl virus (TYLCV). Pymetrozine was applied as Fulfill
TM
50 WG at two rates (0.291 and 0.582 g formulationl
−1) to tomato seedlings with four to six true leaves. Viruliferous whiteflies (three to five per plant) were added 1, 4, 7 and
11 d after a single application of pymetrozine, and transmission rates were determined 4 wk after the addition of whiteflies.
Pymetrozine provided protection against transmission of TYLCV by viruliferous whiteflies for up to 1 wk after a single apliation.
No phytotoxicity was observed on tomato transplants. These results indicate that pymetrozine could be an effective tool for
tomato transplant producers to protect susceptible transplants from infection by begomoviruses, such as TYLCV. Pymetrozine
might also work well as part of an integrated approach to begomovirus management in greenhouse tomato fruit production.
http://www.phytoparasitica.org positing Oct. 20, 2003. 相似文献
6.
Tri Asmira Damayanti Desmiarti Susilo Siti Nurlaelah Dewi Sartiami Tetsuro Okuno Kazuyuki Mise 《Journal of General Plant Pathology》2008,74(6):438-442
Severe mosaic with leaf malformation and green vein banding was observed on yam bean in West and Central Java, Indonesia.
Virions of the causal virus were flexuous filaments, about 700 nm in length, with a coat protein of 30 kDa. The virus was
transmitted by mechanical inoculation and by aphids in a nonpersistent manner. The nucleotide sequence of the coat protein
gene had the highest identity with that of Bean common mosaic virus (BCMV, genus Potyvirus) isolate VN/BB2-5. Based on demarcation criteria, including the genome sequence and host range, we tentatively designate
this isolate as BCMV-IYbn (Indonesian yam bean).
The nucleotide sequence reported is available in the DDBJ/EMBL/GenBank databases under accession number AB289438. 相似文献
7.
Pepper mottle virus, genus Potyvirus, was first identified in Japan based on particle morphology, host range, aphid transmission, and molecular classification using the nucleotide sequence of the coat protein gene and 3-untranslated region. 相似文献
8.
E. G. Borroto-Fernandez T. Sommerbauer E. Popowich A. Schartl M. Laimer 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):171-174
Attempts to conserve and utilise autochthonous grapevine germplasm in modern breeding programmes, are sometimes faced with
the challenge that virus-free plants of old grapevine varieties and clones are hard to find. From 50 year-old vineyards in
Frankonia the Vitis vinifera cv. Domina was selected showing particularly interesting loose-bunch architecture with fewer berries. However this valuable
germplasm was carrying an Arabis mosaic virus (ArMV) infection requiring a reliable and effective method to produce healthy mother plants for clonal selection. Somatic
embryogenesis was established from anthers as the most promising technical approach. The absence of ArMV in 46 regenerated
plant lines was confirmed by ELISA and IC-RT PCR, repeated after different time intervals in vitro and in vivo after acclimatisation, and after one dormancy period under glasshouse conditions. Morphologically, all grapevines appeared
true-to-type, and a screening of 20 plants by flow cytometry to determine the ploidy level and to exclude the risk of undesired
genetic variability confirmed that all tested plants were diploid. Field evaluations of the initially selected bunch traits
are currently underway. 相似文献
9.
Hiroshi Kajihara Kazuyuki Muramoto Shin-ichi Fuji Shuhei Tanaka Shin-ichi Ito 《Journal of General Plant Pathology》2009,75(1):72-75
To detect Japanese yam mosaic virus (JYMV) and Yam mild mosaic virus (YMMV) in yam plants in Japan, we developed a duplex RT-PCR assay consisting of a tube-capture procedure followed by one-step
RT-PCR with two primer pairs. A 241-bp fragment of the coat protein region of JYMV and a 174-bp fragment of the nuclear inclusion
protein b region of YMMV were amplified, thus identifying the two viruses from yam plants cultivated in Yamaguchi Prefecture
in 2007. All water yam plants examined were infected with YMMV alone. All the Japanese yam and Chinese yam plants were infected
with either JYMV alone or both JYMV and YMMV, suggesting that YMMV and JYMV are prevalent among field-grown yam plants. 相似文献
10.
The genomic fragments of two open reading frames (ORFs) 1 and 2 of German and Canadian PAV isolates of Barley yellow dwarf virus (BYDV-PAV) were sequenced. Sequences only slightly differed from previously published sequences of this virus. Two polyclonal antisera against proteins encoded by ORFs 1 and 2 of a German ASL-1 isolate were developed using recombinant antigens expressed in E. coli as a fusion either to His6− or thioredoxin-tags. In Western blot analysis with total protein extracts from BYDV infected plants, antisera efficiently recognized the 99 kDa fusion protein expressed from ORF1 and ORF2 (P1–P2 protein). Later in infection the P1–P2 protein disappeared and two smaller proteins, revealing sizes of 39 and 60 kDa, could be detected. 相似文献
11.
Crude ethanolic seed extracts ofAnnona muricata, A. squamosa (Annonaceae),Lansium domesticum andSandoricum koetjape (Meliaceae) collected from different locations and years in Maluku, Indonesia, were screened for inhibition of larval growth
against the polyphagous lepidopteranSpodoptera litura (Noctuidae). Extracts ofA. squamosa were significantly more active (20-fold) than those ofA. muricata. A. squamosa collected from Namlea yielded the extracts with the greatest inhibitory activity. There were significant differences among
locations for bothA. squamosa andA. muricata but not forL. domesticum andS. koetjape. Extracts ofA. squamosa, collected from Namlea, inhibited larval growth in a dose-dependent manner, with a dietary EC50 (effective concentration to inhibit growth by 50% relative to controls) of 191.7 ppm fresh weight. Extracts ofA. squamosa collected from individual trees in Namlea also varied in growth inhibitory effect againstS. litura andTrichoplusia ni larvae. This species is a candidate for development of a botanical insecticide for local use in Indonesia.
http://www.phytoparasitica.org posting Dec. 1, 2003. 相似文献
12.
Mikiko Harada Satoshi Ishikawa Tadaaki Hibi Kyoko Watanabe 《Journal of General Plant Pathology》2008,74(4):341-343
In 2003–2004, anthracnoses of Enkianthus campanulatus and Rhynchosia acuminatifolia were found for the first time in Kanagawa Prefecture and Tokyo in Japan. These pathogens were identified as Colletotrichum gloeosporioides based on their pathogenicity, morphology and ribosomal DNA spacer sequences.
Results were presented at the annual meeting of The Phytopathological Society of Japan in 2004. 相似文献
13.
Keisuke Tomioka Yuuri Hirooka Takayuki Aoki Toyozo Sato 《Journal of General Plant Pathology》2008,74(3):264-266
Severe rot of leaves, peduncles and flowers caused by Gibberella zeae (anamorph: Fusarium
graminearum) was found on potted plants of hyacinth (Hyacinthus orientalis), a liliaceous ornamental, in greenhouses in Kagawa Prefecture, Japan, in January 2001. This disease was named “Fusarium
rot of hyacinth” as a new disease because only the anamorph, F.
graminearum, was identified on the diseased host plant.
The authors contributed equally to this work.
The fungal isolate and its nucleotide sequence data obtained in this study were deposited in the Genebank, National Institute
of Agrobiological Sciences and the DDBJ/EMBL/GenBank databases under the accession numbers MAFF239499 and AB366161, respectively. 相似文献
14.
Yongxiang Zhang Ewen M. Callaway Jeffrey B. Jones Mark Wilson 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(3):379-390
The plasmid pUFZ75 conferred constitutive GFP expression on the bacterial pathogen Xanthomonas euvesicatoria (syn. X. campestris pv. vesicatoria). Colonisation of the tomato phyllosphere and invasion of tomato leaves by X. euvesicatoria was examined using both fluorescence and confocal laser scanning microscopy. Xanthomonas euvesicatoria established a limited population on the tomato leaf surface, primarily occupying the depressions between epidermal cells
and around the stomata, prior to invasion of the leaf via the stomata and subsequent growth within the substomatal chamber
and the leaf apoplast. Additionally, hrp-gfp fusions were used to report on the temporal and spatial expression of hrp genes during epiphytic colonisation and invasion. Xanthomonas euvesicatoria cells carrying hrpG- and hrpX-gfp reporter constructs were not fluorescent in vitro on non-hrp-inducing LB agar but did exhibit a low level of fluorescence on the leaf surface within 24 h of inoculation, particularly
in the vicinity of stomata. Cells carrying hrpG- and hrpX-gfp fusions exhibited high levels of fluorescence 72 h after inoculation in the substomatal chamber and the leaf apoplast. Cells
carrying the hrpF-gfp fusion were slightly fluorescent on LB agar and showed no further increase in fluorescence on the leaf surface by 24 h after
inoculation, but did show a significant increase in fluorescence 72 h after inoculation in the substomatal chamber and apoplast.
The apparent low-level induction of the regulators hrpG and hrpX on the tomato leaf surface may suggest that some of the genes of the X. euvesicatoria HrpG/HrpX regulon are up- or down-regulated prior to invasion of the stomata while still on the leaf surface. 相似文献
15.
<Emphasis Type="Italic">Turnip yellow mosaic virus</Emphasis> isolated from Chinese cabbage in Japan
Namiko Kirino Koji Inoue Koji Tanina Yuya Yamazaki Satoshi T. Ohki 《Journal of General Plant Pathology》2008,74(4):331-334
A virus that caused a distinct yellow mosaic was isolated in Okayama, Japan from Chinese cabbage (Brassica rapa L., Pekinensis group). The virus, with spherical particles ca. 28 nm in diameter, was mechanically transmissible only to
cruciferous species. From the host range, characteristic morphology of virus particles, serology and sequence analysis of
coat protein gene, the causal virus was identified as Turnip yellow mosaic virus (TYMV). Seed transmission of TYMV at 0–2.2% in Chinese cabbage was confirmed. This report is the first of TYMV from Chinese
cabbage and in Japan.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB358971 and AB358972. 相似文献
16.
Masashi Fujinaga Hideki Ogiso Hidetada Wakabayashi Toshiyuki Morikawa Tomohide Natsuaki 《Journal of General Plant Pathology》2009,75(2):157-159
A viral disease was found in Nagano Prefecture, Japan, on statice (Limonium sinuatum) with chlorotic leaf spot, necrotic stunt, and dwarfing. Spherical virus particles 30 nm in diameter were isolated from infected
plants and statice seedlings and caused identical symptoms 4 weeks after mechanical inoculation. Nucleotide and deduced amino
acid sequences of the coat protein showed 98% and 98.7% identities with those of Grapevine Algerian latent virus (GALV) nipplefruit strain. This is the first report in Japan of a viral disease on statice caused by GALV.
The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank databases under accession AB461854. 相似文献
17.
Shirin Farzadfar Yasuhiro Tomitaka Mutsumi Ikematsu Ali Reza Golnaraghi Reza Pourrahim Kazusato Ohshima 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):45-55
Eight provinces of Iran were surveyed during 2003–2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the
presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were
found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important
weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes.
Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5
isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the
mid-Eurasia of Iran. 相似文献
18.
Irena Mavrič Pleško Mojca Viršček Marn Saša Širca Gregor Urek 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(3):261-268
In 2003, Raspberry bushy dwarf virus was found for the first time in grapevine. Since this was the first report of a non-Rubus natural host, information about it is sparse. During this study the grapevine isolates were characterised biologically, serologically
and genetically and compared with known information about Rubus isolates. Infected plant material was used for mechanical inoculation of test plants, and for serological characterisation
with monoclonal antibodies. Most of RNA 2 was sequenced and compared with other sequences from the database. Grapevine isolates
infected Chenopodium murale systemically, which is not known for raspberry isolates. They were differentiated from Slovenian raspberry isolates with
three monoclonal antibodies using TAS-ELISA. Phylogenetic analyses clustered grapevine isolates separately from raspberry
isolates. Additionally, the virus was detected using nested RT-PCR in Longidorus juvenilis nematodes collected in an infected vineyard. Grapevine isolates of RBDV are distinct from raspberry isolates. 相似文献
19.
The clustered hrp genes encoding the type III secretion system in the Japanese strains MAFF301237 and MAFF311018 of Xanthomonas oryzae pv. oryzae were sequenced and compared. The strains differ in their pathogenicity, location, and year of isolation. A 30-kbp sequence comprising 29 open reading frames (ORFs) was identical in its structural arrangement in both strains but differed from X. campestris pv. campestris, X. axonopodis pv. citri, and X. axonopodis pv. glycines in certain genes located between the hpaB-hrpF interspace region. The DNA sequence and the putative amino acid sequence in each ORF was also identical in both X. oryzae pv. oryzae strains as were the PIP boxes and the relative sequences. These facts clearly showed that the structure of the hrp gene cluster in X. oryzae pv. oryzae is unique. 相似文献
20.
Among the factors affecting the quality and yield of garlic production, blue mold caused by -- Penicillium spp. -- is responsible for economical losses in many countries. Allicin, present in garlic bulbs, has been suggested as having antifungal activity against some Penicillium species. This study was conducted to evaluate the response of garlic accessions against Penicillium hirsutum infection and to compare this response with bulb allicin content. Twelve garlic accessions were inoculated with P. hirsutum, and assayed in greenhouse and growth chamber experiments. Plant growth parameters and the fungal production of conidia were evaluated. Significant differences were found among the accessions. Accessions Castaño and Morado were most resistant whereas AR-I-125 and Fuego were always severely affected by the disease. A low correlation was found (r = 0.17) between allicin content and tolerance, indicating that allicin is not the main factor involved in the resistance against P. hirsutum. 相似文献