A radioimmunoassay for porcine intrauterine folate binding protein.

A RIA was developed for porcine intrauterine folate binding protein (FBP). Displacement of [125I]FBP caused by increasing dilutions of uterine flushings collected from either d-15 pregnant or nonpregnant gilts or media from culture of endometrial tissue from d-15 pregnant or nonpregnant gilts was parallel to the displacement caused by the standard curve. Addition of known amounts of purified allantoic fluid FBP to dilutions of either intrauterine flushings or endometrial culture medium were measured accurately with the RIA. To test specificity, 2-mL samples of uterine flushings collected from d-15 pregnant and nonpregnant gilts were preincubated with 10 microCi of [3H]folic acid and then chromatographed using Sephadex G-100 (Sigma Chemical Co., St. Louis, MO). The fractions were subsequently assayed for radioactivity by liquid scintillation spectrophotometry and for FBP by RIA. The [3H]folic acid and FBP peaks coincided, indicating that the RIA is specific for FBP. Uterine flushings were collected on d 10, 11, 12, 13, 14, and 15 of the cycle or pregnancy from 1) White crossbred, 2) progesterone-treated White crossbred (200 mg of progesterone at 48 and 72 h after estrus), and 3) Meishan gilts and assayed for FBP. Total FBP increased 140-fold from d 10 to 15, and the pattern of change across day did not differ between pregnant and nonpregnant gilts. Progesterone treatment increased intrauterine FBP content on d 10 and 11. No difference in FBP concentrations was detected between White crossbred and Meishan gilts. These results indicate that the RIA for FBP is valid, allowing measurement of this protein in uterine flushings and endometrial culture medium. The onset of FBP secretion by the uterus between d 10 and 15 of the cycle or pregnancy is influenced by the timing of onset of progesterone influence in a manner similar to the endometrial proteins uteroferrin and retinol binding protein. In contrast to these endometrial proteins, FBP concentrations are similar in Meishan and White crossbred gilts.     

对肉鸡业的认识和建议

一 我国肉禽生产的组织化进展情况 1 肉鸡品种结构不断调整，市场布局区域化日益明显 由于具有品种多、肉嫩、味美等优势，符合长江流域及其以南地区和港澳地区的消费需求，近年来我国黄羽肉鸡饲养量以每年10％的速度增长。已经形成区域优势明显、产业特点突出、市场份额不断扩大、市场竞争力不断增强的产业，     

A radioimmunoassay for the detection of antibodies to Mycoplasma gallisepticum

A radioimmunoassay technique was developed to determine the antibodies to Mycoplasma gallisepticum (MG) in sera, yolk fluids, and tracheal washings.     

秦皇岛地区十个鸡场鸡球虫种类的初步调查

通过对秦皇岛地区10个养鸡场鸡球虫种类的调查，发现8个养鸡场为球虫阳性，共检出6种球虫，分别是哈氏美耳球虫（34％）、柔嫩艾美球虫（26％）、早熟艾美耳球虫（12％）、和缓艾美耳球虫（12％）、堆形艾美耳球虫（11％）和毒害艾美耳球虫（5％）。     

鸡肉毒梭菌中毒的诊治

我镇某散养鸡场，07年6月28目，2月龄2000只鸡群中突然发生运动神经麻痹和延脑麻痹为主要症状的疾病，同时发生大批死亡。前后共发病928只，发病率46．4％，死亡886只，病死率89．7％。通过发病情况调查，临诊症状观察，病理剖检及动物播种试验，初步诊断为肉毒梭菌毒系中毒症。     

文山大种鸡品种简介

文山大种鸡是云南省优良的地方禽种,具有性情温顺、觅食能力强、耐粗饲、适应性强、抗逆性强、抗病力强,早期生长速度较快,肉质鲜美,阉鸡肥育期增重及屯肥能力较好,屠宰率较高等诸多优点.     

7个地方鸡种的分子评估

成熟的基因扩增及测序技术,结合比较发达的网络与信息技术,给我们提供了以DNA为基础的系统分类方法.一种新的DNA分类方法--DNA条形编码(DNA Barcoding)技术应运而生.本研究以线粒体细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidase Ⅰ,CD Ⅰ)基因的特定序列作为DNA条形码,对我国7个地方鸡种进行分子评估.研究结果表明:选择的这段CO Ⅰ基因序列有22个突变位点,为13个单倍型,其中11个单倍型为各品种所特有;6个鸡种有其特异位点,这些特异单倍型和特异位点作为DNA条形码可以对其进行分子评估,并可以作为辅助各品种鉴定的依据.7个品种间Kimura双参数遗传距离为0.017～0.389.7个品种的DNA分类和形态学分类基本一致,该基因可以探讨地方鸡种分类问题.     

肉鸡饲养管理技术

1　饮水雏鸡进入围拦略加休息后 ,即可给水。建议前 3～ 7ｄ用凉开水。前 1～ 2ｄ可在水中加入 5%葡萄糖和 0 1 %ＶＣ ,以提高成活率 ,加多维电解盐类药物可缓解脱水及长途应激。育雏前几天 ,饮水器盛水量宜少 ,每天更换 1～ 2次 ,并进行清洗 ,水温与舍温接近。随着日龄的增长 ,供应充足清洁的饮用水。每天需水量见表 1。注意事项 :①非长途运输的雏鸡 ,葡萄糖只加一次即可 ,第二次换水时停用 ,否则会造成消化不良、增加糊肛鸡的数量、卵黄吸收延缓 ,不利以后的生长。②发生呛水时 ,可增加饮水器的数量 ,多移动几次饮水器 ,并提高舍温 1～ …     

High-sensitivity radioimmunoassay screening method for fentanyl

A radioiodinated analog of fentanyl was synthesized for use with a commercially available radioimmunoassay for fentanyl. The sensitivity of the modified assay was at least 100 times greater than that of the original assay. Using this modified assay, concentrations of fentanyl as low as 1 pg/ml of fentanyl or fentanyl equivalents in equine urine were detected. Doses of fentanyl 100 times smaller than the minimum dose for a pharmacologic effect were detectable and a pharmacologically effective dose of fentanyl was detectable for up to 96 hours or more. The high sensitivity of the assay indicated that large numbers of urine samples (ie, 10 to 20) probably could be pooled and screened simultaneously, which would result in an economical analysis for fentanyl in the urine of horses after a race. Sufentanil and its metabolites also were detectable, using this assay, but at only about 1% of the efficiency at which fentanyl was detectable.     

Development of a radioimmunoassay for isometamidium

A radioimmunoassay for the trypanocidal drug isometamidium (ISMM) has been developed using antibodies produced in sheep immumzed against the drug cross-linked to human serum albumin. With ¹⁴C-ISMM as a radioligand, cross-reactivity of the antibodies was determined and found to be less than 3% with pyrithidium, diminazene and quinapyramine and about 13% with homidium. The limit of detection of ISMM in serum without prior extraction was 5.8 pmol/tube and within-assay and between-assay coefficients of variation were four and 14% respectively.Deceased.     

鸡白介素17A的克隆表达及活性测定

从经人工感染柔嫩艾美尔球虫孢子化卵囊(1×104个/只鸡)的盲肠上皮间淋巴细胞(IELs)提取总RNA,用RT-PCR方法成功扩增了鸡白介素17A(IL-17A)基因,测序结果显示,开放阅读框为453bp,编码150个氨基酸,与GenBank上鸡IL-17A基因(AM773756)的核苷酸和氨基酸序列完全一致(100%),与报道的哺乳动物IL-17A的氨基酸相似性达37%～46%。构建的pGEX-6p1-chIL-17A原核表达载体经1mmol/L IPTG诱导后表达出43kDa左右的融合蛋白,与预期大小一致,且Western-blot鉴定表达产物为目的蛋白。功能试验表明,表达的重组鸡IL-17A能够刺激鸡胚成纤维细胞产生IL-6。这些结果表明,鸡IL-17A在结构和功能方面与哺乳动物的IL-17A都有一定的相似性,可能在鸡的免疫应答过程中起到重要的作用。     

Characterization of two microsatellites in chicken monoamine oxidase A

We surveyed two polymorphic microsatellites in a candidate gene for fear response, monoamine oxidase A ( MAOA ), in chicken. Two hundred and eighty chickens from five breeds (Nagoya, Mikawa, White Leghorn, White Plymouth Rock, and Rhode Island Red) were investigated. A thymine (T) repeat and an adenine (A) repeat were found on MAOA intron 4 ( CMin4T ) and intron 9 ( CMin9A ), respectively. Nine alleles ( 127-bp to 166-bp ) in CMin4T and six alleles ( 184-bp to 198-bp ) in CMin9A were detected. A 128-bp allele in CMin4T was observed in the Nagoya breed only, implying a highly useful marker for discriminating the Nagoya breed from other breeds. In addition, the Mikawa breed had the fixed 127-bp and 198-bp alleles in CMin4T and CMin9A , respectively. The Nagoya breed chickens show densely cowardly behavior, but the Mikawa breed chickens do not show the same behavior. The current results may indicate that MAOA is an informative candidate gene for breed difference.     

改良凝集试验(MAT)检测鸡弓形虫血清抗体

本研究是在Dubey J P所建改良凝集试验基础上,采用小鼠肉瘤S180细胞培养弓形虫速殖子,甲醛固定抗原后悬浮于碱性缓冲液,用2-巯基乙醇处理待测血清,用该法对610份鸡血清进行弓形虫抗体检测,并用间接血凝试验(IHA)作对照。结果显示,MAT、IHA对鸡血清的弓形虫抗体检测阳性率分别为9.02%(55/610)、1.64%(10/610),二者差异极显著(P<0.01)。MAT对鸡的弓形虫抗体检测结果,鸡血清阳性率为9.02%(55/610),其中散养鸡阳性率为18.75%(30/160),笼养鸡阳性率为5.56%(25/450),二者差异极显著(P<0.01);蛋鸡和种鸡阳性率分别为7.89%(15/190)和8.00%(8/100)、肉鸡阳性率1.25%(2/160),与肉鸡相比差异显著(P<0.05)。     

广西鸡传染性贫血流行病学的研究

应用PCR检测技术 ,对采自广西五个主要养鸡地区 5 0多个鸡群 3 2 1羽病鸡的组织样品 ,进行了鸡传染性贫血病毒核酸的检测。结果检测的病例平均阳性率为 2 8 3 4%;三黄鸡的阳性检出率最高 ( 3 9 2 8%) ;各种日龄的鸡均可检出 ,其中最小的为 7日龄、最大的为 3 5 0日龄 ;对 5个免疫器官的检测结果显示 ,骨髓的检出率最高( 84 3 8%) ;CIAV阳性病例中与MDV、REV和ALV的混合感染率共计 48 40 %。     

腿部变化与鸡病鉴别论断

在饲养过程中有很多难以鉴别的疾病，鸡腿病就是其中的一种。鸡腿病主要是由遗传、营养、传染和环境等因素所致。轻者造成鸡生长受阻，影响增重，型者则终身残疾，造成较大的经济损失。