The conservation of Chinese rice biodiversity: genetic erosion, ethnobotany and prospects

As one of important original centers of Asian cultivated rice Oryza sativa L., China is abundant in genetic resources of rice and its wild relatives. They are not only useful for mountainous farmers to cope with heterogeneous microclimates, but also of extremely significance for rice genetic improvement programs in the future. Unfortunately, the combination of new conditions, including rapid population growth, new agricultural technology and swift economic & cultural changes produces a new environment. As a result, most of wild populations have disappeared, and others are at the edge of the extinction. Moreover, widespread adoption of high-yielding rice varieties (HYVs) have led to biological impoverty of rice germplasms, as local rice varieties are abandoned for modern varieties. These processes, known as genetic erosion, are generally summarized as two types in Chinese rice genetic resources. Based on our recent studies on the conservation of Chinese rice biodiversity, this paper reviews the biodiversity, ethnobotany and genetic erosion of rice and its wild relatives in China. Finally, some suggestions for the ways to enhance efforts to conserve rice genetic resources, including investigation of genetic erosion, studies on population genetics and conservation genetics using molecular approaches, settings of in situ & ex situ conservation priorities, significance of ethnobotanical knowledge for taking in situ conservation, and necessities of extensive participation in the conservation activities, are given.     

Genetics of a butterfly relocation: large, small and introduced populations of the mountain endemic Erebia epiphron silesiana

Species specific levels of genetic diversity are necessary for the viability of populations. Erebia epiphron (Lepidoptera: Satyrinae) is a relic species in the Sudetan Mountains, Czech Republic. These populations represent a suitable study object to test the population genetic effects of large, small and introduced populations by means of allozyme electrophoresis. A large continuous and viable population (>100,000 individuals) shows a significantly higher genetic diversity than a small isolated and declining population (ca. 5000 individuals), which most probably was founded during the Little Ice Age in the 17th and 18th centuries and became isolated afterwards. In 1932 and 1933, 50 females were transferred from the large population to the Krkonose Mountains, where they established a viable and quickly expanding population. The number of transferred individuals was sufficient to transfer most of the allozyme diversity of the large natural population. Due to the higher elevation of the Krkonose in comparison to the native range, this allocation might safeguard the survival of the Czech race of E. epiphron even under conditions of global warming.     

Determining Sources of Diversity in Cultivated and Wild <Emphasis Type="Italic">Lablab purpureus</Emphasis> Related to Provenance of Germplasm by using Amplified Fragment Length Polymorphism

To improve understanding of diversity of Lablab purpureus and establish relationships among 103 germplasm accessions collected from diverse geographic origins, amplified fragment length polymorphism markers were used. Four primer sets selected out of 16 produced 289 clear, repeatable polymorphisms. UPGMA analysis of similarity data clustered the accessions according to their subspecific taxonomic organization, i.e., subsp. purpureus and subsp. uncinatus, as well as to cultivated and wild forms. The well-represented landraces from Africa and Asia, belonging predominantly to subsp. purpureus, displayed moderate genetic diversity. Wild forms from Africa showed far greater levels of diversity that would justify taxonomic re-assessment of the wild subsp. uncinatus. The molecular analysis identified forms that were collected in the wild in India but were genetically placed intermediate between wild and cultivated forms. As these plant types did not exist among the African accessions, it is suggested that they might represent escapes from early attempts of domestication. These results support the suggested pathway of domestication and distribution of L. purpureus from Africa to Asia. Additional members to a previously published core collection of the species are proposed.     

Farmers' knowledge and perceptions of soil erosion and conservation measures in the Central Highlands,Kenya

A lack of appreciation of Kenyan farmers' knowledge and their perceptions of soil erosion and soil conservation measures was the reason for low adoption of recommended technologies. This research was carried out to identify the criteria that farmers used to distinguish farm‐types and to use these types to evaluate different knowledge levels and perceptions of soil erosion and existing soil and water conservation (SWC) measures in the Central Highlands of Kenya. Community meetings and semi‐structured household surveys were carried out in a small catchment, with 120 households. Results partly support the idea of using farmer‐developed criteria to distinguish among land managers with reference to a farm‐type classification. Criteria distinguishing three classes of land managers (good, moderate and poor) were significant with regard to the following land husbandry practices: use of hybrid or recycled seed and use of organic and/or inorganic fertilizers. Farmers were aware of on‐going soil erosion and of several erosion control measures. Whereas a majority of farmers preferred grass‐strips as a SWC measures, they did not recognize agroforestry as a form of SWC measure. Farmers perceived that SWC measures could successfully increase crop yields and soil‐water retention, and increase land value. In general, farmers did not perceive that SWC measures successfully prevented erosion phenomena, given the evidence of on‐site erosion indicators. They attributed the continued erosion to high rainfall, steep slopes, lack of maintenance and poorly designed SWC measures. They did not consider poor soil‐cover, up‐down tillage and tall trees to be the causes of erosion. Farmers faced several constraints in adopting SWC measures: lack of labour, tools, capital and know‐how to construct the measures. Copyright © 2005 John Wiley & Sons, Ltd.     

Conservation strategies and management guidelines for wild Prunus genetic resources in Andalusia, Spain

Six species of the genus Prunus occur inAndalusia. Matters regarding their ecology, ethnobotanical aspectsand conservation are discussed, as well as knowledge about thegermplasm of those species with greater economic importance and theirdegree of conservation, namely: P.avium, P.mahaleb and P.insititia. Various conservation measures areproposed for wild germplasm and for the germplasm of local varieties:a) conservation efforts should target the best populations,except in the case of P.avium and P.insititia, where every individual should beaddressed, including both wild and local cultivars; b) theDehesa del Camarate (Sierra Nevada) is proposed as an areafor in situ germplasm conservation; c) theawareness of the importance of Prunus germplasmshould be increased among forest workers, environmental managers andthe public; d) some silvicultural techniques are proposed, suchas mixed Prunus patches, hedge treatment,Z-tree selection at the early stages and the protection ofroot suckers; e) improved, selected P.avium and P.mahaleb germplasm should be used at highlyproductive sites since these two species can be cultivated as highvalue timber trees; f) some Prunus speciescan also provide profitable NTFPs in the region. Details regardingdifferent collection areas are covered. Finally, some topics arerecommended for scientific research. Three tables and a distributionmap of Prunus species in Andalusia areincluded.     

Land Degradation Controlled and Mitigated by Rubber‐based Agroforestry Systems through Optimizing Soil Physical Conditions and Water Supply Mechanisms: A Case Study in Xishuangbanna,China

Different rubber‐based agroforestry systems are adopted to control and mitigate land degradation. However, the soil physical conditions and soil hydrological processes of different agroforestry systems are still unclear. Thus, in this study, rubber (Hevea brasiliensis ) monoculture, rubber and Clerodendranthus spicatus agroforestry system (RCS) and rubber and Amomum villosum agroforestry system (RAV) were developed from a degraded land that had similar backgrounds of terrain and management measures for 50 years. Conventional methods were applied, and dye tracer experiments were conducted to measure the soil physical conditions and determine the water movement in soil. After 5 years' growth, both RCS and RAV could effectively promote the soil physical conditions and optimize soil structure by improving the proportion of the three soil phases. Favourable soil properties, multiple‐layered canopies and ground cover in agroforestry systems could promote the formation of three‐dimensional hydraulic redistribution in soil profile. The infiltration of rainfall into the soil was enhanced; meanwhile, surface runoff and soil erosion were mitigated, and then more water was transported, redistributed and stored into the different soil layers by the more dominant preferential flow, water exchange and lateral flow in soil profiles. These water supply mechanisms could allow planting intercrops with rubber trees to uptake water from different water sources and coexist in an agroforestry system. Our results highlighted that rubber‐based agroforestry systems are a useful management practice to maximize the utilization of land and water resources. Copyright © 2017 John Wiley & Sons, Ltd.     

Changes in seed Morphology, Dormancy and Germination from wild to Cultivated Hyacinth bean Germplasm (Lablab purpureus: Papilionoideae)

Morphological and physiological seed characteristics of the hyacinth bean (Lablab purpureus) were investigated in a set of 18 different germplasm accessions, from wild over semi-domesticated forms to landraces and current cultivars. The objective of this study was to improve the understanding of the domestication of this tropical legume crop. Wild accessions were easily distinguished by morphological characters, such as small, greyish-brown, mottled seeds. Cultivated or semi-domesticated forms showed a much wider variation in size, colour and shape. Seed mass of cultivated accessions reached up to almost 10 times that of wild accessions. Most wild accessions showed a greater spread of germination and larger proportions of hard seeds, up to about 70%, than most cultivated and so-called semi-domesticated accessions. Cluster analysis applied separately to morphological and germination data and a subsequent discriminant analysis did not help integrating the morphological variability observed. The presumed occurrence of semi-domesticated accessions within the germplasm tested as well as the crop's origin from Africa alone or both Africa and Asia are debated on the basis of seed germination and hard seed coat dormancy.     

AFLP fingerprinting of Anchusa (Boraginaceae) in the Corso-Sardinian system: Genetic diversity, population differentiation and conservation priorities in an insular endemic group threatened with extinction

Although several plants endemic to Corsica and Sardinia are included in various redlists, no attempts have been made to analyse their genetic diversity with molecular techniques. Genus Anchusa occurs with seven taxa in either mountain or coastal habitats of the two islands, but the very restricted range and low population size pose these endemisms in a very precarious conservation status. Highly variable markers (AFLP) were therefore used to analyse the patterns and levels of genetic diversity in a sample of 11 populations from the entire range of the group.Results indicate the separation between a mountain genic pool including Anchusa formosa, Anchusa capellii and Anchusa montelinasana, and four groups of coastal accessions. In spite of small size, mountain taxa show low interpopulation differentiation (F_st = 0.02) and relatively high intrapopulation genetic variation (0.365), while coastal accessions showed on average a stronger differentiation (mean F_st = 0.20) and a lower diversity (0.281), possibly due to higher rates of inbreeding. The particularly low levels of variation found in A. sardoa, A. littorea and A. crispa ssp. maritima from the Coghinas bay are likely due to a historical decrease of populations and to bottleneck events caused by loss of habitat and natural stochastic factors on sand dune ecosystems. While habitat maintenance and regulation of grazing by domestic herbivores should be sufficient to ensure the persistence of the mountain endemics, additional actions of in situ and ex situ conservation are needed for the critically endangered coastal species A. sardoa and A. littorea. A. crispa showed a relatively high variation, especially on Corsica. No correlation between population size and genetic variation was found in the latter species, highlighting the importance of the small patches for its conservation. Also, the genetic separation between subspecies crispa and maritima stresses the need of keeping them distinct in redlists and conservation actions on Sardinia.